IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ fair-2001-1 Protein interactions of the MLL PHD fingers modulate MLL target gene regulation in human cells. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Fair K., Anderson M., Bulanova E., Mi H., Tropschug M., Diaz MO. Apoptosis - Interactions involving proteins with a function related to apoptosis Mol. Cell. Biol. (0270-7306) 2001 fair-2001-2 Protein interactions of the MLL PHD fingers modulate MLL target gene regulation in human cells. in vitro In vitro pull down pull down predetermined predetermined participant predetermined Fair K., Anderson M., Bulanova E., Mi H., Tropschug M., Diaz MO. Apoptosis - Interactions involving proteins with a function related to apoptosis Mol. Cell. Biol. (0270-7306) 2001 fair-2001-3 Protein interactions of the MLL PHD fingers modulate MLL target gene regulation in human cells. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Fair K., Anderson M., Bulanova E., Mi H., Tropschug M., Diaz MO. Apoptosis - Interactions involving proteins with a function related to apoptosis Mol. Cell. Biol. (0270-7306) 2001 fair-2001-4 Protein interactions of the MLL PHD fingers modulate MLL target gene regulation in human cells. in vitro In vitro pull down pull down polyclonal western polyclonal antibody western blot polyclonal western Fair K., Anderson M., Bulanova E., Mi H., Tropschug M., Diaz MO. Apoptosis - Interactions involving proteins with a function related to apoptosis Mol. Cell. Biol. (0270-7306) 2001 trx_drome Histone-lysine N-methyltransferase trithorax KMT2A Lysine N-methyltransferase 2A trx CG8651 Cysteine methyltransferase KMT2A protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MGRSKFPGKPSKSINRKRISVLQLEDDAANPAEPQQPAPESQQPSGSGSGSSAAREKGNNCDNDEDDNAPGGASISGNTASSSAGSGNSGNGSSSGSSTGSGSSGSGSTNGGSVNGGTHHKSAANLDKEAVTKDQNGDGDKTRGNVSSAPSGKLSAAASGKALSKSSRTFSASTSVTSSGRSSGSSPDGNSGASSDGASSGISCGKSTAKSTEASSGKLAKTTGAGTCSSAKSSKASSGTTSEATTSGLSGACLKALFVATPATSTGLACALVSPGGSSQGGTFPISAALLRARKNSNKKFKNLNLARGEVMLPSTSKLKQLNSPVVDNPSPSPPIASGSTPSVEGGIGVGGVVSPGEDAALKRVLTEMPNEVARDPSPSSCTAAANGAASGKGSASNGPPAMASSGDGSSPKSGADTGPSTSSTTAKQKKTVTFRNVLETSDDKSVVKRFYNPDIRIPIVSIMKKDSLNRPLNYSRGGECIVRPSILSKILNKNSNIDKLNSLKFRSAGASSSSSNQESGSSSNVFGLSRAFGAPMDEDDEGGVTFRRNDSPEDQNNAEDDEMDDDDDDEEAEEDDENEDDNDEAVSEKSAETEKSAGADERDPDEKQLVMDSHFVLPKRSTRSSRIIKPNKRLLEEGAISTKKPLSLGDSKGKNVFGTSSSSAGSTASTFSASTNLKLGKETFFNFGTLKPNSSAAGNFVLRQPRLQFQADNQQATFTAPKACPTSPSAIPKPANSLATSSFGSLASTNSSTVTPTPSACSICSAVVSSKEVTQARKYGVVACDVCRKFFSKMTKKSISANSSTANTSSGSQQYLQCKGNEGSPCSIHSAKSQLKNFKKFYKDRCTACWLKKCMISFQLPAAHRSRLSAILPPGMRGEAAAREEKSAELLSPTGSLRFTSTASSSSPSVVASTSVKWKSSGDSTSALTSIKPNPLAENNVTFGSTPLLRPAILENPLFLKISNAADQKLAAAEAISPSLTKKNSKQEKEKVKESEQSEKLLSPTQAGTKKSGAAEAQVEEVQPQKEEAPQTSTTTQPSASNGASHGVPQAELAGETNATGDTLKRQRIDLKGPRVKHVCRSASIVLGQPLATFGEDQQPEDAADMQQEIAAPVPSAIMEPSPEKPTHIVTDENDNCASCKTSPVGDESKPSKSSGSAQAEVKKATALGKEGTASAAGGSSAKVTTRNAAVASNLIVAASKKQRNGDIATSSSVTQSSNQTQGRKTKEHRQQRTLISIDFWENYDPAEVCQTGFGLIVTETVAQRALCFLCGSTGLDPLIFCACCCEPYHQYCVQDEYNLKHGSFEDTTLMGSLLETTVNASTGPSSSLNQLTQRLNWLCPRCTVCYTCNMSSGSKVKCQKCQKNYHSTCLGTSKRLLGADRPLICVNCLKCKSCSTTKVSKFVGNLPMCTGCFKLRKKGNFCPICQRCYDDNDFDLKMMECGDCGQWVHSKCEGLSDEQYNLLSTLPESIEFICKKCARRNESSKIKAEEWRQAVMEEFKASLYSVLKLLSKSRQACALLKLSPRKKLRCTCGASSNQGKLQPKALQFSSGSDNGLGSDGESQNSDDVYEFKDQQQQQQQRNANMNKPRVKSLPCSCQQHISHSQSFSLVDIKQKIAGNSYVSLAEFNYDMSQVIQQSNCDELDIAYKELLSEQFPWFQNETKACTDALEEDMFESCSGGNYEDLQDTGGVSASVYNEHSTSQAESRSGVLDIPLEEVDDFGSCGIKMRLDTRMCLFCRKSGEGLSGEEARLLYCGHDCWVHTNCAMWSAEVFEEIDGSLQNVHSAVARGRMIKCTVCGNRGATVGCNVRSCGEHYHYPCARSIDCAFLTDKSMYCPAHAKNGNALKANGSPSVTYESNFEVSRPVYVELDRKRKKLIEPARVQFHIGSLEVRQLGAIVPRFSDSYEAVVPINFLCSRLYWSSKEPWKIVEYTVRTTIQNSSSTLTALDVGRNYTVDHTNPNSKEVQLGMAQIARWHTSLARSEFLENGGTDWSGEFPNPNSCVPPDENTEEEPQQQADLLPPELKDAIFEDLPHELLDGISMLDIFLYDDKTDLFAISEQSKDGTQAMTSNQAQNQNQQAGGANSVSICDEDTRNSNTSLGNGWPASNPVEDAMLSAARNSSQVQMLKTLAWPKLDGNSAMATAIKRRKLSKNLAEGVFLTLSSQQRNKKEMATVAGVSRRQSISETSVEGVATTSGSVRSKSFTWSAAKRYFEKSEGREEAAKMRIMQMDGVDDSITEFRIISGDGNLSTAQFSGQVKCDRCQCTYRNYDAFQRHLPSCSPTMSSNETESDVSGQGMTNNATQISAESLNELQKQLLANAGGLNYLQSATSFPQVQSLGSLGQFGLQGLQQLQLQPQSLGSGFFLSQPNPATQANTDDLQIYANSLQSLAANLGGGFTLAQPTVTAPAQPQLIAVSTNPDGTQQFIQIPQTMQATTTPTATYQTLQATNTDKKIMLPLTAAGKPLKTVATKAAQQAAVKQRQLKSGHQVKPIQAKLQPHPQQHQQQQQTQVQQPITVMGQNLLQPQLLFQSSTQTQAPQIILPQAQPQNIISFVTGDGSQGQPLQYISIPTAGEYKPQPQPTATPTFLTTAPGAGATYLQTDASGNLVLTTTPSNSGLQMLTAQSLQAQPQVIGTLIQPQTIQLGGGADGNQPGSNQQPLILGGTGGGSSGLEFATTSPQVILATQPMYYGLETIVQNTVMSSQQFVSTAMPGMLSQNASFSATTTQVFQASKIEPIVDLPAGYVVLNNTGDASSAGTFLNAASVLQQQTQDDTTTQILQNANFQFQSVPTSSGASTSMDYTSPVMVTAKIPPVTQIKRTNAQAKAAGISGVGKVPPQPQVVNKVLPTSIVTQQSQVQVKNSNLKQSQVKGKAASGTGTTCGAPPSIASKPLQKKTNMIRPIHKLEVKPKVMKPTPKVQNQNHSLLQQQQQQQPQLQQQIPAVVVNQVPKVTISQQRIPAQTQQQQLQQAQMIHIPQQQQPLQQQQVQVQPSMPIITLAEAPVVQSQFVMEPQALEQQELANRVQHFSTSSSSSSSNCSLPTNVVNPMQQQAPSTTSSSTTRPTNRVLPMQQRQEPAPLSNECPVVSSPTPPKPVEQPIIHQMTSASVSKCYAQKSTLPSPVYEAELKVSSVLESIVPDVTMDAILEEQPVTESIYTEGLYEKNSPGESKTEQLLLQQQQREQLNQQLVNNGYLLDKHTFQVEPMDTDVYREEDLEEEEDEDDDFSLKMATSACNDHEMSDSEEPAVKDKISKILDNLTNDDCADSIATATTMEVDASAGYQQMVEDVLATTAAQSAPTEEFEGALETAAVEAAATYINEMADAHVLDLKQLQNGVELELRRRKEEQRTVSQEQEQSKAAIVPTAAAPEPPQPIQEPKKMTGPHLLYEIQSEDGFTYKSSSITEIWEKVFEAVQVARRAHGLTPLPEGPLADMGGIQMIGLKTNALKYLIEQLPGVEKCSKYTPKYHKRNGNVSTAANGAHGGNLGGSSASAALSVSGGDSHGLLDYGSDQDELEENAYDCARCEPYSNRSEYDMFSWLASRHRKQPIQVFVQPSDNELVPRRGTGSNLPMAMKYRTLKETYKDYVGVFRSHIHGRGLYCTKDIEAGEMVIEYAGELIRSTLTDKRERYYDSRGIGCYMFKIDDNLVVDATMRGNAARFINHCCEPNCYSKVVDILGHKHIIIFALRRIVQGEELTYDYKFPFEDEKIPCSCGSKRCRKYLN kmt2a_human Histone-lysine N-methyltransferase 2A KMT2A ALL-1 ALL1 CXXC-type zinc finger protein 7 CXXC7 HRX HTRX MLL MLL1 Myeloid/lymphoid or mixed-lineage leukemia Myeloid/lymphoid or mixed-lineage leukemia protein 1 TRX1 Trithorax-like protein Zinc finger protein HRX Cysteine methyltransferase KMT2A protein protein human Homo sapiens Human MAHSCRWRFPARPGTTGGGGGGGRRGLGGAPRQRVPALLLPPGPPVGGGGPGAPPSPPAVAAAAAAAGSSGAGVPGGAAAASAASSSSASSSSSSSSSASSGPALLRVGPGFDAALQVSAAIGTNLRRFRAVFGESGGGGGSGEDEQFLGFGSDEEVRVRSPTRSPSVKTSPRKPRGRPRSGSDRNSAILSDPSVFSPLNKSETKSGDKIKKKDSKSIEKKRGRPPTFPGVKIKITHGKDISELPKGNKEDSLKKIKRTPSATFQQATKIKKLRAGKLSPLKSKFKTGKLQIGRKGVQIVRRRGRPPSTERIKTPSGLLINSELEKPQKVRKDKEGTPPLTKEDKTVVRQSPRRIKPVRIIPSSKRTDATIAKQLLQRAKKGAQKKIEKEAAQLQGRKVKTQVKNIRQFIMPVVSAISSRIIKTPRRFIEDEDYDPPIKIARLESTPNSRFSAPSCGSSEKSSAASQHSSQMSSDSSRSSSPSVDTSTDSQASEEIQVLPEERSDTPEVHPPLPISQSPENESNDRRSRRYSVSERSFGSRTTKKLSTLQSAPQQQTSSSPPPPLLTPPPPLQPASSISDHTPWLMPPTIPLASPFLPASTAPMQGKRKSILREPTFRWTSLKHSRSEPQYFSSAKYAKEGLIRKPIFDNFRPPPLTPEDVGFASGFSASGTAASARLFSPLHSGTRFDMHKRSPLLRAPRFTPSEAHSRIFESVTLPSNRTSAGTSSSGVSNRKRKRKVFSPIRSEPRSPSHSMRTRSGRLSSSELSPLTPPSSVSSSLSISVSPLATSALNPTFTFPSHSLTQSGESAEKNQRPRKQTSAPAEPFSSSSPTPLFPWFTPGSQTERGRNKDKAPEELSKDRDADKSVEKDKSRERDREREKENKRESRKEKRKKGSEIQSSSALYPVGRVSKEKVVGEDVATSSSAKKATGRKKSSSHDSGTDITSVTLGDTTAVKTKILIKKGRGNLEKTNLDLGPTAPSLEKEKTLCLSTPSSSTVKHSTSSIGSMLAQADKLPMTDKRVASLLKKAKAQLCKIEKSKSLKQTDQPKAQGQESDSSETSVRGPRIKHVCRRAAVALGRKRAVFPDDMPTLSALPWEEREKILSSMGNDDKSSIAGSEDAEPLAPPIKPIKPVTRNKAPQEPPVKKGRRSRRCGQCPGCQVPEDCGVCTNCLDKPKFGGRNIKKQCCKMRKCQNLQWMPSKAYLQKQAKAVKKKEKKSKTSEKKDSKESSVVKNVVDSSQKPTPSAREDPAPKKSSSEPPPRKPVEEKSEEGNVSAPGPESKQATTPASRKSSKQVSQPALVIPPQPPTTGPPRKEVPKTTPSEPKKKQPPPPESGPEQSKQKKVAPRPSIPVKQKPKEKEKPPPVNKQENAGTLNILSTLSNGNSSKQKIPADGVHRIRVDFKEDCEAENVWEMGGLGILTSVPITPRVVCFLCASSGHVEFVYCQVCCEPFHKFCLEENERPLEDQLENWCCRRCKFCHVCGRQHQATKQLLECNKCRNSYHPECLGPNYPTKPTKKKKVWICTKCVRCKSCGSTTPGKGWDAQWSHDFSLCHDCAKLFAKGNFCPLCDKCYDDDDYESKMMQCGKCDRWVHSKCENLSDEMYEILSNLPESVAYTCVNCTERHPAEWRLALEKELQISLKQVLTALLNSRTTSHLLRYRQAAKPPDLNPETEESIPSRSSPEGPDPPVLTEVSKQDDQQPLDLEGVKRKMDQGNYTSVLEFSDDIVKIIQAAINSDGGQPEIKKANSMVKSFFIRQMERVFPWFSVKKSRFWEPNKVSSNSGMLPNAVLPPSLDHNYAQWQEREENSHTEQPPLMKKIIPAPKPKGPGEPDSPTPLHPPTPPILSTDRSREDSPELNPPPGIEDNRQCALCLTYGDDSANDAGRLLYIGQNEWTHVNCALWSAEVFEDDDGSLKNVHMAVIRGKQLRCEFCQKPGATVGCCLTSCTSNYHFMCSRAKNCVFLDDKKVYCQRHRDLIKGEVVPENGFEVFRRVFVDFEGISLRRKFLNGLEPENIHMMIGSMTIDCLGILNDLSDCEDKLFPIGYQCSRVYWSTTDARKRCVYTCKIVECRPPVVEPDINSTVEHDENRTIAHSPTSFTESSSKESQNTAEIISPPSPDRPPHSQTSGSCYYHVISKVPRIRTPSYSPTQRSPGCRPLPSAGSPTPTTHEIVTVGDPLLSSGLRSIGSRRHSTSSLSPQRSKLRIMSPMRTGNTYSRNNVSSVSTTGTATDLESSAKVVDHVLGPLNSSTSLGQNTSTSSNLQRTVVTVGNKNSHLDGSSSSEMKQSSASDLVSKSSSLKGEKTKVLSSKSSEGSAHNVAYPGIPKLAPQVHNTTSRELNVSKIGSFAEPSSVSFSSKEALSFPHLHLRGQRNDRDQHTDSTQSANSSPDEDTEVKTLKLSGMSNRSSIINEHMGSSSRDRRQKGKKSCKETFKEKHSSKSFLEPGQVTTGEEGNLKPEFMDEVLTPEYMGQRPCNNVSSDKIGDKGLSMPGVPKAPPMQVEGSAKELQAPRKRTVKVTLTPLKMENESQSKNALKESSPASPLQIESTSPTEPISASENPGDGPVAQPSPNNTSCQDSQSNNYQNLPVQDRNLMLPDGPKPQEDGSFKRRYPRRSARARSNMFFGLTPLYGVRSYGEEDIPFYSSSTGKKRGKRSAEGQVDGADDLSTSDEDDLYYYNFTRTVISSGGEERLASHNLFREEEQCDLPKISQLDGVDDGTESDTSVTATTRKSSQIPKRNGKENGTENLKIDRPEDAGEKEHVTKSSVGHKNEPKMDNCHSVSRVKTQGQDSLEAQLSSLESSRRVHTSTPSDKNLLDTYNTELLKSDSDNNNSDDCGNILPSDIMDFVLKNTPSMQALGESPESSSSELLNLGEGLGLDSNREKDMGLFEVFSQQLPTTEPVDSSVSSSISAEEQFELPLELPSDLSVLTTRSPTVPSQNPSRLAVISDSGEKRVTITEKSVASSESDPALLSPGVDPTPEGHMTPDHFIQGHMDADHISSPPCGSVEQGHGNNQDLTRNSSTPGLQVPVSPTVPIQNQKYVPNSTDSPGPSQISNAAVQTTPPHLKPATEKLIVVNQNMQPLYVLQTLPNGVTQKIQLTSSVSSTPSVMETNTSVLGPMGGGLTLTTGLNPSLPTSQSLFPSASKGLLPMSHHQHLHSFPAATQSSFPPNISNPPSGLLIGVQPPPDPQLLVSESSQRTDLSTTVATPSSGLKKRPISRLQTRKNKKLAPSSTPSNIAPSDVVSNMTLINFTPSQLPNHPSLLDLGSLNTSSHRTVPNIIKRSKSSIMYFEPAPLLPQSVGGTAATAAGTSTISQDTSHLTSGSVSGLASSSSVLNVVSMQTTTTPTSSASVPGHVTLTNPRLLGTPDIGSISNLLIKASQQSLGIQDQPVALPPSSGMFPQLGTSQTPSTAAITAASSICVLPSTQTTGITAASPSGEADEHYQLQHVNQLLASKTGIHSSQRDLDSASGPQVSNFTQTVDAPNSMGLEQNKALSSAVQASPTSPGGSPSSPSSGQRSASPSVPGPTKPKPKTKRFQLPLDKGNGKKHKVSHLRTSSSEAHIPDQETTSLTSGTGTPGAEAEQQDTASVEQSSQKECGQPAGQVAVLPEVQVTQNPANEQESAEPKTVEEEESNFSSPLMLWLQQEQKRKESITEKKPKKGLVFEISSDDGFQICAESIEDAWKSLTDKVQEARSNARLKQLSFAGVNGLRMLGILHDAVVFLIEQLSGAKHCRNYKFRFHKPEEANEPPLNPHGSARAEVHLRKSAFDMFNFLASKHRQPPEYNPNDEEEEEVQLKSARRATSMDLPMPMRFRHLKKTSKEAVGVYRSPIHGRGLFCKRNIDAGEMVIEYAGNVIRSIQTDKREKYYDSKGIGCYMFRIDDSEVVDATMHGNAARFINHSCEPNCYSRVINIDGQKHIVIFAMRKIYRGEELTYDYKFPIEDASNKLPCNCGAKKCRKFLN ppie_human Peptidyl-prolyl cis-trans isomerase E Cyclophilin E Cyclophilin-33 PPIE CYP33 Rotamase E protein protein human Homo sapiens Human MATTKRVLYVGGLAEEVDDKVLHAAFIPFGDITDIQIPLDYETEKHRGFAFVEFELAEDAAAAIDNMNESELFGRTIRVNLAKPMRIKEGSSRPVWSDDDWLKKFSGKTLEENKEEEGSEPPKAETQEGEPIAKKARSNPQVYMDIKIGNKPAGRIQMLLRSDVVPMTAENFRCLCTHEKGFGFKGSSFHRIIPQFMCQGGDFTNHNGTGGKSIYGKKFDDENFILKHTGPGLLSMANSGPNTNGSQFFLTCDKTDWLDGKHVVFGEVTEGLDVLRQIEAQGSKDGKPKQKVIIADCGEYV trx-trx-1 Homodimerization of Drosophila trx, as detected by Y2H. 182841 N/A 182845 unspecified role unspecified role prey prey phd1-3 domains binding region binding-associated region binding region range range range range false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 182845 unspecified role unspecified role bait bait phd1-3 domains binding region binding-associated region binding region range range range range false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association false false false mll-mll-1 Interaction of human MLL, as detected by Y2H. 182841 N/A 182846 unspecified role unspecified role prey prey human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 N/A 182846 unspecified role unspecified role bait bait phd1-3 domains binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association false false false mll-mll-2 Interaction of human MLL, as detected by affinity coimmunoprecipitation. 182842 N/A 182846 unspecified role unspecified role bait bait phd1-3 domain binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false c-terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) N/A 182846 unspecified role unspecified role prey prey phd1-4 domain binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false 35s tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro physical association physical association false false false ppie-cyp33-1 Interaction of Cyp33 and MLL, as detected by Y2H. 182843 N/A 182847 unspecified role unspecified role prey prey human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 N/A 182846 unspecified role unspecified role bait bait phd3 domain binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association false false false ppie-mll-2 Interaction of Cyp33 and MLL, as detected by GST pull down. 182844 N/A 182847 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro N/A 182846 unspecified role unspecified role prey prey phd1-4 domains binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false 35s tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro physical association physical association false false false