IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ feng-2000-1 LIS1 regulates CNS lamination by interacting with mNudE, a central component of the centrosome. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Feng Y., Olson EC., Stukenberg PT., Flanagan LA., Kirschner MW., Walsh CA. Apoptosis - Interactions involving proteins with a function related to apoptosis cwalsh@caregroup.harvard.edu A mouse E10.5 total embryonic cDNA library was used. Neuron (0896-6273) 2000 Only protein-protein interactions imex curation feng-2000-3 LIS1 regulates CNS lamination by interacting with mNudE, a central component of the centrosome. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Feng Y., Olson EC., Stukenberg PT., Flanagan LA., Kirschner MW., Walsh CA. Apoptosis - Interactions involving proteins with a function related to apoptosis cwalsh@caregroup.harvard.edu A mouse E10.5 total embryonic cDNA library was used. Neuron (0896-6273) 2000 Only protein-protein interactions imex curation feng-2000-5 LIS1 regulates CNS lamination by interacting with mNudE, a central component of the centrosome. human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Feng Y., Olson EC., Stukenberg PT., Flanagan LA., Kirschner MW., Walsh CA. Apoptosis - Interactions involving proteins with a function related to apoptosis cwalsh@caregroup.harvard.edu A mouse E10.5 total embryonic cDNA library was used. Neuron (0896-6273) 2000 Only protein-protein interactions imex curation feng-2000-2 LIS1 regulates CNS lamination by interacting with mNudE, a central component of the centrosome. human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Feng Y., Olson EC., Stukenberg PT., Flanagan LA., Kirschner MW., Walsh CA. Apoptosis - Interactions involving proteins with a function related to apoptosis cwalsh@caregroup.harvard.edu A mouse E10.5 total embryonic cDNA library was used. Neuron (0896-6273) 2000 Only protein-protein interactions imex curation feng-2000-4 LIS1 regulates CNS lamination by interacting with mNudE, a central component of the centrosome. human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Feng Y., Olson EC., Stukenberg PT., Flanagan LA., Kirschner MW., Walsh CA. Apoptosis - Interactions involving proteins with a function related to apoptosis cwalsh@caregroup.harvard.edu A mouse E10.5 total embryonic cDNA library was used. Neuron (0896-6273) 2000 Only protein-protein interactions imex curation feng-2000-6 LIS1 regulates CNS lamination by interacting with mNudE, a central component of the centrosome. in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Feng Y., Olson EC., Stukenberg PT., Flanagan LA., Kirschner MW., Walsh CA. Apoptosis - Interactions involving proteins with a function related to apoptosis cwalsh@caregroup.harvard.edu A mouse E10.5 total embryonic cDNA library was used. Neuron (0896-6273) 2000 Only protein-protein interactions imex curation feng-2000-7 LIS1 regulates CNS lamination by interacting with mNudE, a central component of the centrosome. chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 fluorescence imaging fluorescence microscopy fluorescence imaging immunostaining immunostaining Feng Y., Olson EC., Stukenberg PT., Flanagan LA., Kirschner MW., Walsh CA. Apoptosis - Interactions involving proteins with a function related to apoptosis cwalsh@caregroup.harvard.edu A mouse E10.5 total embryonic cDNA library was used. Neuron (0896-6273) 2000 Only protein-protein interactions imex curation nudc_mouse Nuclear migration protein nudC Nuclear distribution protein C homolog Silica-induced gene 92 protein Nudc protein protein mouse Mus musculus Mouse MGGEQEEERFDGMLLAMAQQHEGGVQELVNTFFSFLRRKTDFFIGGEEGMAEKLITQTFNHHNQLAQKARREKRARQETERREKAERAARLAKEAKAETPGPQIKELTDEEAERLQLEIDQKKDAEDQEAQLKNGSLDSPGKQDAEDEEDEEDEKDKGKLKPNLGNGADLPNYRWTQTLAELDLAVPFRVSFRLKGKDVVVDIQRRHLRVGLKGQPPVVDGELYNEVKVEESSWLIEDGKVVTVHLEKINKMEWWNRLVTSDPEINTKKINPENSKLSDLDSETRSMVEKMMYDQRQKSMGLPTSDEQKKQEILKKFMDQHPEMDFSKAKFN lis1_human Platelet-activating factor acetylhydrolase IB subunit beta PAF acetylhydrolase 45 kDa subunit PAF-AH alpha Lissencephaly-1 protein PAFAH1B1 LIS1 MDCR PAFAHA MDS protein protein human Homo sapiens Human MVLSQRQRDELNRAIADYLRSNGYEEAYSVFKKEAELDVNEELDKKYAGLLEKKWTSVIRLQKKVMELESKLNEAKEEFTSGGPLGQKRDPKEWIPRPPEKYALSGHRSPVTRVIFHPVFSVMVSASEDATIKVWDYETGDFERTLKGHTDSVQDISFDHSGKLLASCSADMTIKLWDFQGFECIRTMHGHDHNVSSVAIMPNGDHIVSASRDKTIKMWEVQTGYCVKTFTGHREWVRMVRPNQDGTLIASCSNDQTVRVWVVATKECKAELREHEHVVECISWAPESSYSSISEATGSETKKSGKPGPFLLSGSRDKTIKMWDVSTGMCLMTLVGHDNWVRGVLFHSGGKFILSCADDKTLRVWDYKNKRCMKTLNAHEHFVTSLDFHKTAPYVVTGSVDQTVKVWECR nde1_mouse Nuclear distribution protein nudE homolog 1 Nde1 Nude protein protein mouse Mus musculus Mouse MEDSGKTFESEEEETNYWRDLAMTYKQRAENTQEELREFQEGSREYEAELEAQLQQIETRNRDLLSENNRLRMELESVKEKFEMQHSEGYRQISALEDDLAQTKAIKDQLQKYIRELEQANDDLERAKRATIMSLEDFEQRLNQAIERNAFLESELDEKENLLESVQRLKDEARDLRQELAVQQKQDKPRTPMPGSGQAKRTDMAVQATGSVPSTPVAHRGPSSGLNTPGMFRRGLDSSTSGTPLTPAARISALNIVGDLLRKVGALESKLASCRNFMYDQSPSRTSGPASGRGTKNRDGVDRRPGSTSVGDKGSGKRLEFGKPASEPASPALPSAQGVVKLLL pcnt_mouse Pericentrin Pcnt Pcnt2 protein protein mouse Mus musculus Mouse MEDEQEQRRRKVEAGRAKLANFRQRKTKGDCPNSKKKTAKRKGSAVHASVQEEGSVATPNSELPQGGAVFESPSCSNTLEGTRGASAAQEQEDCELDVTDLQGQQQTQPPPPQTAHSLELEALRLSLNNMHTAQLELTQANLQKEKETALTELREMLNGRRAQELALLQSRQQCELELLREQHAREKEEMALRSGQEAAELKEKLRSEMEKNAQTIETLKQDWESERELCLENLRQELSLKHQSEMEGLQSQFQKELSEQKVELEKIFQAKHEAEVSLKNLEAQHQAAIKKLQEDLQSEHCQYLQDLEQKFREKEKAKELELETLQASYEDLKAQSQEEIRLLWSQLESMKTNREELNGSWDPVLAQASHLEELEHLRSGFAQQQQQERAQHESELEHLRVYFEKKLKDAEKTYQEDLTVFQQRLQEAREDSLESTEISSSCVLPEETSGREGKEPPDPLDLQLGQPKVQESLVEDCQVKLSKAEEKIQQMKEEFQKKEAEWELSREELKREAEERLASMFLELREKAESEKLSIISRFEHRESSMRHLQDQQAAQILDLERSLMEQQGHLRQLEQELTRDDLLPCSQCGQEPAMAQEEKNGALLREKEDCALQLLMAQNRFLEERKEIMEKFAKEQDAFLRDAQEKHNHELQLLQQGHQQQLLALRMELETKHRSELTEQLASSESRRQALLETHVAELQVKHNAEISALEKRHLSNLDELESCYVADVQTIRDEHQQALELLRAELEEQLQKKESCHREMLTQELENLKRQHAEELQSVRDSLRMEMSAQHIENGKGPAADLQGAHQQDPAMALHNEGHLLVEDGDAVLRSVDAEGLLHQAGPQELGDAHTVEMQKSQAELAKPQELQASQDQVAQVRDKVFLLNRELEECRAELEQLQQRRERENQEGTTLICMLRADLELAQGEGKALRDALRRLLDLFGDTLKAAVTLKSRISERAGLLLDHEDAADTSDARLAAAALGDMWSDEGLLEIDRTLPEGAETSSVCEISSHVCESFFISPENTLDCEQPIRRVYQSLSTAVEGLLEMALDSSKQLEEARQLHRCVEREFRHRNEEMAQAMQKQQELLERLREESAAKDRLALELHTAKGLLEGFKVEKVDLQEALGKKEESEQQLILELEDLRKQLQQAARELLTLKEEKSVLWNQKETLTNEAKEREAALQEEVESLTRVQWESRKQSEKDRATLLSQMRVLESELEDQLVQHRGCAQLAEEVATLKQQLAALDKHLRSQRQFMDDQAAEREHEREEFQQEIQRLEGQLRQAARPRPPGPRDSQCVQLDEEVELLQEKLREKLDGFNELVIKKDFADQQLLIQEEEIKRLEETNASIQRQMVQLQEELEKQKKSMEELKEKEILKQENMGDLLLTTVSRSGLDEAGCPMLPQGSSSRGPEAQPDVTERALLQHENEVVHRRNSEIDELKSLIENLQENQRQLQKDKAEEIEQLHEVIEKLQSELSLMGPKVHEVSDPQAGSLHSELACLRGEGLGGQALRSELQAAQAAKEVFGQLLANQAHGHSQALEALQQRLQDAEEVAARHLAELEHCVALREAEVEAMASQIQEFAATLKAKEAIIEQRDLEIDAVNKWKVSHSLELEAILLALAHFRHALEQQTCATPDEPPELRQLRVQCARLSHQLQVLYRPFLKCRMQLDQHQPHVASIGCANPCADDELEQEGVSNRLALAPHSLAAQAKEELEDCPLGKANLMAQVRQLQEELDHRVHSVASRDTNSETCKLQQPNLSENGPRNHCCNGEESKPSPPDDVLNIAKTTWDVIDIIKNQDLLVQVEMPDFPTQEKLTSQGGPFSSQASGHSGSLLPEEAAEPQQDPVRALDLSSWSSPEVVRKDPSLEPQHSLPLTPGVGTVSLHSVDISPDWTDPLLQADVSGLLCYPGKSASGQAPLWAVAPSAGKHHAERTATEKDVEDFIVTSFDSQELLTSPSHELARRSDGSRKSDGPDIAMMLTLGSEGSETPTTDLVAAAAAAVPFSRRFVQSPGAMKEKEIHAKQMKALLQMVFDESHQILALSESQDPSSALNKGEPRDPLDGFPRDSQALSEVTTDKGEKESLETHLTWSEELLRAIQEVFAREQEKAELQPRPYGSNLGDYNSLVQRLEKVIQEQGDPQKVQDHLCLSDRSSLLAEIQALRAQLRMTHLQNQEKLQQLCAALTSTEARGSQREHQLRRQVELLAYKVEQEKCIANELQKTLSKEQETASDVRKRLVVEQNAVQDLKSELHACKQENTSLLESLDKVQQEVLRLRAVLDGKEKELKVVLEELESERGKGQALQAQQEEQQLRYLQREGQSSRALEELKLSLEKQLAQNNQLCVALKHERAAKDNLQKELQIEASRCEALLAQEKGQLSELQKSLEAERSRSLELSEALQHERLLTEQLSRNSQEACARQETQVQHALLRKLKAEKTRALELEAMLEKVQKQAAHTQQQLEAQAQERCVELRREKERELEIQRQRDEHKIEQLQRLVRELRWKEEVSGGNGPCRGSPGRGSLERDQFQEQQQELEKIRQQLLCAAGLLTSFTNHTVDRTIKDWTSSNEKAVSSLMRTLEELKSELSMPTSFQKKMTAELQVQLMNELLSDNDALTKAVGMATREKAELCRTVSRLEKTLKHHTQKGCVLNRQSKSSLKQDGTDLQSSLRHSDPEWHSQTTSGDTNTCNIKMEKLYLHYLRAESFRKALIYQKKYLLLLIGGFQDSEQETLSMIAHLGVFPSKADKKITMSRPFTKFRTAVRVVIAVLRLRFLVKKWQEVDRKGALVHPKSTRHGHRTSQRQRSPSGPRASLPTRDTSSGPTKASRHSPRSAAAGSPGKERSTSTPSSRLERSLTASQDPEHSLTEYIHHLEMIQQRLGGLPPDSTQKSCHQKIKQ q155p7_mouse Cenpf Lek1 protein protein mouse Mus musculus Mouse MSWALEEWKEGLPSRALQKIQELEGQLEKLKKEKQQRQFQLDSLEAALQKQKQKVEDGKTEGADLKRENQRLMEICEHLEKSRQKLSHELQVKESQVNLQESQLSSCKKQIEKLEQELKRCKSEFERSQQVAQSADVSLNPCSTPQKLFATPLTPSSTYEDLKEKYNKEVEERKRLEEEVKALHAKKVSLPVSQATMNHRDIARHQASSSVFPWQQENTPSRLSSDALKTPLRRDGSAAHFLGEEVSPNKSSMKTGRGDCSSLPGEPHSAQLLHQAKAQNQDLKSKMTELELRLQGQEKEMRSQVNKCQDLQLQLEKTKVELIEKERILNKTRDEVVRSTAQYDQAAAKCTTLEQKLKTLTEELSCHRQNAESAKRSLEQRIKEKEKELQEELSRQHQSFQALDSEYTQMKTRLTQELQQVKHLHSTLQLELEKVTSVKQQLERNLEEIRLKLSRAEQALQASQVAENELRRSSEEMKKENSLIRSQSEQRTREVCHLEEELGKVKVSLSKSQNFAEEMKAKNTSQEIMLRDLQEKLNQQENSLTLEKLKLALADLERQRNCSQDLLKKREHHIDQLNNKLNKIEKEFETLLSALELKKKECEELKEEKNQISFWKIDSEKLINQIESEKEILLGKINHLETSLKTQQVSPDSNERIRTLEMERENFTVEIKNLQSMLDSKMVEIKTQKQAYLELQQKSESSDQKHQKEIENMCLKANKLTGQVESLECKLQLLSSEVVTKDQQYQDLRMEYETLRDLLKSRGSSLVTNEDNQRSSEDNQRSSEDNQRGSLAFEQQPAVSDSFANVMGRKGSINSERSDCSVDGGRSPEHIAILQNRVTSLESSLESQNQMNSDLQMRCEELLQIKGEVEENLSKAEQIHQNFVAETNQCISKLQEDAAVHQNIVAETLATLESKEKELQLLKEKLEAQQTEVQKLNKNNCLLEGTLKELQLLSDTLSSEKKEMNSIISLSKKNIEELTQANEALKEVNEALEQEKMNLLQKHEKITSCIAEQERSIAELSDQYKQERLQLLQRCEETEAVLEDLRGNYKTAQENNAKLECMLSECTALCENRKNELEQLKETFAKEQQEFLTKLAFAEEQNRKLMLELEIEQQTVRSEITNTNKHSMSATDGLRQECLTLNEEQNEQQNEVSNLTHENEQLMELTQTKHDSYLAVEPVENSVKATEDEIGKSSSQYQMDIDTKDISLDSYKAQLVHLEALVRILEVQLDQSEEENKKLHLELQTIREELETKSSQDPQSQARTGLKDCDTAEEKYVSMLQELSASQNENAHLQCSLQTAVNKLNELGKMCDVLRVEKLQLESELNDSRTECITATSQMTAEVEKLVSEMKMLNHESALSQNELMKDTSGGEFHDKANHSSVFLTPLDSSNFCEQMTLSSKEVRVHFAELQEKFSCLQSEHKILHDQHCEVSSKMSALRSYVDTLKAENSALSMSLRTLQGDLVKEGEPAAEGGHGLPLSFCGADSPSLTNFGETSFYKDVLEQTGDTCHLSLEGNASANSCDLDEEFSSSLEEETLTEKESPPAPGRTVEGLEVLCQVYLQSLKNLEEKTESQRIMKNKEIEELEQLLSSERKELSCLRKQYLSEKEQWQQKLTSVTLEMESKLAEEKQQTKTLSLELEVARLQLQELDLSSRSLLGTDLESVVRCQNDNYDIKESEVYISETTEKTPKQDTDQTCDKDIQQDLGLETSVTESETTRLTGEGCEEQPPKTNCEAPAEDKTQDCSECISELCSSSNVLVPMDVLEDQGSIQNLQLQKDTLNENLRLLPEVEDWDKKVESLLNEIMEADSKLSLQEVQLKMKIATCIQLEKIVKDLRKEKADLSEKLESLPCNQEVCLRVERSEEDLGFNLDMGANELLSKSTKDNATNTEDNYKEKFLDMERELTRIKSEKANIEHHILSVETNLEVVQAEKLCLERDTESKQKVIIDLKEELFTVISERNRLREELDNVSKESKALDQMSKKMKEKIEELESHQRESLRHIGAVESEVKDKADLIQTLSFNVGELTKDKAHLQEQLQNLQNDSQELSLAIGELEIQIGQLNKEKESLVKESQNFQIKLTESECEKQTISKALEVALKEKGEFAVQLSSAQEEVHQLRRGIEKLSVRIEADEKKHLSAVAKLKESQRESDSLKDTVETLERELERSEENQELAILDSENLKAEVETLKAQKDEMTKSLRIFELDLVTVRTERENLAKQLQEKQSRVSELDERCSSLRRLLEEKEQARVQMEEDSKSAMLMLQMQLKELREEVAALCNDQETLKAQEQSLDQPGEEVHHLKSSIRKLKVHIDADEKKHQNILEQLKESKHHADLLKDRVENLEQELILSEKNMIFQAEKSKAEIQTLKSEIQRMAQNLQDLQLELISTRSENENLIKELKKEQERVSDLETINPSIENLLKDKEQEKVQMKEEAKITVEMLQTQLKELNETVVSLCNDQEVSKTKEQNLGSQVQTLELEKAQLLQDLGEAKNKYIIFQSSVNALTQEVEAGKQKLEKGEKEIRTLKEQLKSQEQLVCKLAQVEGEQELWQKQKLELRNVTMALEQKVQVLQSENNTLQSTYEALQNSHKSLESELGLIKLEKVALVERVSTISGKEAELQRELRDMLQKTTQLSEDYNKEKNRLTEEVEVLREELQNTKAAHLKSVNQLEKELQRAQGKIKLMLKSCRQLEGEKEMLQKELSQLEAAQQQRAGSLVDSNVDEVMTENKALKETLEEKVKEADKYLDKYCSLLISHEELEKAKEILEIEVARLKSRQSRQDLQSSPLLNSSIPGPSPNTSVSEMKSASGQNKASGKRQRSSGVWEHGKRAAPSTAETFSKKSRKSDSKSTRPAEHEQETEFEPEGLPEVVKKGFADIPTGKTSPYILRRTTMATRTSPRFATQKLVGSSPSLGKENVVESSKPTAGGSRSQKVKVVQESSADSHTAFQELPAKSLTASNIPGRNSTESPREGLRAKRAYPASSPAAGPDPTNNENCRVQ cp110_mouse Centriolar coiled-coil protein of 110 kDa Cep110 Ccp110 Kiaa0419 Cp110 Centrosomal protein of 110 kDa protein protein mouse Mus musculus Mouse MEEYEEFCEKALGRAQEASLSTGSFLPAQAESVSLIRFHGVAVLSPLLTIEKRKKIQEEKQKALDVQSRKQANRKKALLTRVQEILENVQVRKAPNASDFDQWATETIYSNPEVTDLNVPVRVPNSLPSPTEHCTSVKLEKITGLLPVNNEDQQTPKRVGLPGDSEVSGSLRQCESPESRQAEDGAALRLSSASPQETIISDVLGKEEQDPSCLAEVTPDPYIMSLQNLMKRSKEYVERELSSRSLRNSLKRSVNETHSDRENDAAKASDCVKEKAPPMPIGRHCGSAIPDKPSLNKSNVLLQGASQASSMGTAGLASFSKIDLPAGAAPPAAPDAGSDFTVIPTFVTENKVKSLKGPYAKLPSPEPSMSPTMHRRHSRSASACQILINNPVNACELSPKGKEEAVDRTAPAAAETTNESETVPKSPTDLTGVCSSNVSATKITSESTREMVVGKPSQRQQALGAHLGNNVTVERSAMEGPFIADDRGAQKVDGTCMAVPKLHELQPSSQCVSSQTLEDVCELKSASLLAKNSCNLQMELNKSYDVKHPSPLLTQTQTSRQQMDTPPVFRGNEQFVDNSFEKVKRRLDLDVDSLQKENCPYIITAGVAEQERDRLLERRYPKGFVHINKNKMLETSPKEGQELLKSKMLAFEEMRKRLEEQHAQQLSLLIAEQEREQEQLQKEIEEQEKMLKEKAVTTDVSDLNSALEWRQRTDSALLETMLSQVDSLQTSNNSGFITSALQYSFGSAGEAPFYLWGSLTSGVTRVSGTRPCGRAQAKWSQVFNPEIHAKFNKITAVAKGFLTRKLMQTDKLKQLRQTVKDTMEFIRSFQSEAPLKRGVVSAQDASLQERVLAQLRAALYGIHDIFFVMDAAERMSILHHDREARKEKLLRQMDKMKSPRVALSVATQKSLDRKKFMKVAEMGMPNKKFLLKQNPSETRVLQPNQGQNAPVHRLLSRQGTPKTSVKGVVQNRQKPSQSRVPNRAPVSGAYAGKTQRKRPNVATI tcpa_mouse T-complex protein 1 subunit alpha CCT-alpha Tailless complex polypeptide 1B Tcp1 Ccta Cct1 Tailless complex polypeptide 1A protein protein mouse Mus musculus Mouse MEGPLSVFGDRSTGEAVRSQNVMAAASIANIVKSSFGPVGLDKMLVDDIGDVTITNDGATILKLLEVEHPAAKVLCELADLQDKEVGDGTTSVVIIAAELLKNADELVKQKIHPTSVISGYRLACKEAVRYINENLIINTDELGRDCLINAAKTSMSSKIIGINGDYFANMVVDAVLAVKYTDARGQPRYPVNSVNILKAHGRSQIESMLINGYALNCVVGSQGMPKRIVNAKIACLDFSLQKTKMKLGVQVVITDPEKLDQIRQRESDITKERIQKILATGANVILTTGGIDDMYLKYFVEAGAMAVRRVLKRDLKHVAKASGASILSTLANLEGEETFEVTMLGQAEEVVQERICDDELILIKNTKARTSASIILRGANDFMCDEMERSLHDALCVVKRVLELKSVVPGGGAVEAALSIYLENYATSMGSREQLAIAEFARSLLVIPNTLAVNAAQDSTDLVAKLRAFHNEAQVNPERKNLKWIGLDLVHGKPRDNKQAGVFEPTIVKVKSLKFATEAAITILRIDDLIKLHPESKDDKHGSYENAVHSGALDD slap1_mouse Src-like-adapter Sla Slap Slap1 Src-like-adapter protein 1 protein protein mouse Mus musculus Mouse MGNSMKSTSPPSERPLSSSEGLESDFLAVLTDYPSPDISPPIFRRGEKLRVISDEGGWWKAISLSTGRESYIPGICVARVYHGWLFEGLGRDKAEELLQLPDTKIGSFMIRESETKKGFYSLSVRHRQVKHYRIFRLPNNWYYISPRLTFQCLEDLVTHYSEVADGLCCVLTTPCLAQNIPAPTSHPSPCTSPGSPVTLRQKTFDWKRVSRLQEGSEGAENPLRVDESLFSYGLRESIASYLSLTGDDSSSFDRKKKSLSLMYTGSKRKSSFFSAPQYFED dyl1_mouse Dynein light chain 1, cytoplasmic Dynll1 Dlc1 Dnclc1 Dncl1 Dynein light chain LC8-type 1 8 kDa dynein light chain Protein inhibitor of neuronal nitric oxide synthase protein protein mouse Mus musculus Mouse MCDRKAVIKNADMSEEMQQDSVECATQALEKYNIEKDIAAHIKKEFDKKYNPTWHCIVGRNFGSYVTHETKHFIYFYLGQVAILLFKSG tbg1_human Tubulin gamma-1 chain TUBG1 TUBG Gamma-1-tubulin Gamma-tubulin complex component 1 protein protein human Homo sapiens Human MPREIITLQLGQCGNQIGFEFWKQLCAEHGISPEGIVEEFATEGTDRKDVFFYQADDEHYIPRAVLLDLEPRVIHSILNSPYAKLYNPENIYLSEHGGGAGNNWASGFSQGEKIHEDIFDIIDREADGSDSLEGFVLCHSIAGGTGSGLGSYLLERLNDRYPKKLVQTYSVFPNQDEMSDVVVQPYNSLLTLKRLTQNADCVVVLDNTALNRIATDRLHIQNPSFSQINQLVSTIMSASTTTLRYPGYMNNDLIGLIASLIPTPRLHFLMTGYTPLTTDQSVASVRKTTVLDVMRRLLQPKNVMVSTGRDRQTNHCYIAILNIIQGEVDPTQVHKSLQRIRERKLANFIPWGPASIQVALSRKSPYLPSAHRVSGLMMANHTSISSLFERTCRQYDKLRKREAFLEQFRKEDMFKDNFDEMDTSREIVQQLIDEYHAATRPDYISWGTQEQ lis1_mouse Platelet-activating factor acetylhydrolase IB subunit beta PAF acetylhydrolase 45 kDa subunit PAF-AH alpha Lissencephaly-1 protein Pafah1b1 Lis1 Lis-1 Pafaha protein protein mouse Mus musculus Mouse MVLSQRQRDELNRAIADYLRSNGYEEAYSVFKKEAELDMNEELDKKYAGLLEKKWTSVIRLQKKVMELESKLNEAKEEFTSGGPLGQKRDPKEWIPRPPEKYALSGHRSPVTRVIFHPVFSVMVSASEDATIKVWDYETGDFERTLKGHTDSVQDISFDHSGKLLASCSADMTIKLWDFQGFECIRTMHGHDHNVSSVAIMPNGDHIVSASRDKTIKMWEVQTGYCVKTFTGHREWVRMVRPNQDGTLIASCSNDQTVRVWVVATKECKAELREHEHVVECISWAPESSYSSISEATGSETKKSGKPGPFLLSGSRDKTIKMWDVSTGMCLMTLVGHDNWVRGVLFHSGGKFILSCADDKTLRVWDYKNKRCMKTLNAHEHFVTSLDFHKTAPYVVTGSVDQTVKVWECR lis1_cerae_protein protein protein chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey An amino acid sequence which is not represented in UniProt Similar to P63005 lis1-nudc Y2H showing interaction between llis1 and nudc 801243 n/a 801250 unspecified role unspecified role prey prey n/a 801251 unspecified role unspecified role bait bait physical association physical association false false false Fig 1. lis1-nude-1 Y2H showing interaction between llis1 and nude 801243 n/a 801252 unspecified role unspecified role prey prey n/a 801251 unspecified role unspecified role bait bait physical association physical association false false false Fig 1. Lis1 interacts with NudE (nde1). nude-pcnt Y2H showing interaction between nude/nde1 and pericentrin. 801243 n/a 801253 unspecified role unspecified role prey prey n/a 801252 unspecified role unspecified role bait bait physical association physical association false false false Fig. 5A nude-cenpf Y2H showing interaction between nude/nde1 and cenpf 801243 n/a 801254 unspecified role unspecified role prey prey n/a 801252 unspecified role unspecified role bait bait physical association physical association false false false Fig. 6A nude-cep110 Y2H showing interaction between nude/nde1 and cep110 801243 n/a 801255 unspecified role unspecified role prey prey n/a 801252 unspecified role unspecified role bait bait physical association physical association false false false nude-tcp1 Y2H showing interaction between nude/nde1 and tcp1 801243 n/a 801256 unspecified role unspecified role prey prey n/a 801252 unspecified role unspecified role bait bait physical association physical association false false false nude-slap Y2H showing interaction between nude/nde1 and slap 801243 n/a 801257 unspecified role unspecified role prey prey n/a 801252 unspecified role unspecified role bait bait physical association physical association false false false Fig 6A nude-lc8 Y2H showing interaction between nude/nde1 and the 8kDa light chain of cytoplasmic dynein (lc8) 801243 n/a 801252 unspecified role unspecified role bait bait n/a 801258 unspecified role unspecified role prey prey physical association physical association false false false Fig. 6A lis1-nude-4 Y2H showing interaction between llis1 and nude (nde1) 801244 n/a 801251 unspecified role unspecified role bait bait P43034:p.His149Arg mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false P43034:p.Ser169Pro mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false n/a 801252 unspecified role unspecified role prey prey physical association physical association false false false Fig 2C. The sites at amino acid positions 149 and 169 in the lis1 protein play an important role in binding to nude (nde1). nude-lc8-1 Y2H showing interaction between nude/nde1 and the 8kDa light chain of cytoplasmic dynein (lc8) 801244 n/a 801258 unspecified role unspecified role prey prey n/a 801252 unspecified role unspecified role bait bait physical association physical association false false false Fig 6B. nude-tcp1-1 Y2H showing interaction between nude/nde1 and tcp1 801244 n/a 801252 unspecified role unspecified role bait bait n/a 801256 unspecified role unspecified role prey prey physical association physical association false false false Fig 6B. nude-pcnt-1 Y2H showing interaction between nude/nde1 and pericentrin. 801244 n/a 801253 unspecified role unspecified role prey prey n/a 801252 unspecified role unspecified role bait bait physical association physical association false false false Fig 6B. nude-slap-1 Y2H showing interaction between nude/nde1 and slap 801244 n/a 801252 unspecified role unspecified role bait bait n/a 801257 unspecified role unspecified role prey prey physical association physical association false false false Fig 6B. nude-cep110-1 Y2H showing interaction between nude/nde1 and cep110 801244 n/a 801255 unspecified role unspecified role prey prey n/a 801252 unspecified role unspecified role bait bait physical association physical association false false false Fig 6B. nude-cenpf-1 Y2H showing interaction between nude/nde1 and cenpf 801244 n/a 801254 unspecified role unspecified role prey prey n/a 801252 unspecified role unspecified role bait bait physical association physical association false false false Fig 6B. lis1-nude-6 CoIP showing interaction between lis1 and nude 801245 n/a 801252 unspecified role unspecified role bait bait n-terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false n/a 801251 unspecified role unspecified role prey prey n-terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag anti tag western anti tag western blot anti tag western n-term range n-terminal range n-term range n-term range n-terminal range n-term range false P43034:p.His149Arg mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false P43034:p.Ser169Pro mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Fig 2D. Lis1 interacts with NudE (nde1) and the two important amino acid sites at positions 149 and 169 are required for this interaction. lis1-nude-7 CoIP showing interaction between lis1 and nude 801245 n/a 801251 unspecified role unspecified role prey prey region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag anti tag western anti tag western blot anti tag western undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 801252 unspecified role unspecified role bait bait region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Fig 2D. lis1-nude-2 CoIP showing interaction between lis1 and nude 801246 n/a 801252 unspecified role unspecified role bait bait n-terminal myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false n/a 801251 unspecified role unspecified role prey prey physical association physical association false false false Fig 2A. nude-gtubulin CoIP showing interaction between nude and gamma-tubulin 801247 n/a 801259 unspecified role unspecified role bait bait n/a 801252 unspecified role unspecified role prey prey c-terminal gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag anti tag western anti tag western blot anti tag western c-term range C-terminal range c-term range c-term range C-terminal range c-term range false physical association physical association false false false Fig 6A. NudE (nde1) interacts with gamma-tubulin but not with Lis1. lis1-nude-8 CoIP showing interaction between lis1 and nude 801248 n/a 801252 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) n/a 801260 unspecified role unspecified role prey prey direct interaction direct interaction false false false Fig 2B. lis1-nude-9 Colocalisation of Lis1 and Nude 801249 n/a 801261 unspecified role unspecified role neutral component neutral component n/a 801252 unspecified role unspecified role neutral component neutral component region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false colocalization colocalization false false false