IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ rochat_stei-2000-3 FIST/HIPK3: a Fas/FADD-interacting serine/threonine kinase that induces FADD phosphorylation and inhibits fas-mediated Jun NH(2)-terminal kinase activation. human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. coip coimmunoprecipitation co-immunoprecipitation CoIp immunoprecipitation Co-IP co-immunoprecipitation CoIp immunoprecipitation Co-IP coip western blot western blot Immuno blot Immuno blot Rochat-Steiner V., Becker K., Micheau O., Schneider P., Burns K., Tschopp J. Apoptosis - Interactions involving proteins with a function related to apoptosis J. Exp. Med. (0022-1007) 2000 rochat_stei-2000-1 FIST/HIPK3: a Fas/FADD-interacting serine/threonine kinase that induces FADD phosphorylation and inhibits fas-mediated Jun NH(2)-terminal kinase activation. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Rochat-Steiner V., Becker K., Micheau O., Schneider P., Burns K., Tschopp J. Apoptosis - Interactions involving proteins with a function related to apoptosis J. Exp. Med. (0022-1007) 2000 rochat_stei-2000-2 FIST/HIPK3: a Fas/FADD-interacting serine/threonine kinase that induces FADD phosphorylation and inhibits fas-mediated Jun NH(2)-terminal kinase activation. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Rochat-Steiner V., Becker K., Micheau O., Schneider P., Burns K., Tschopp J. Apoptosis - Interactions involving proteins with a function related to apoptosis Directed yeast-2-hybrid J. Exp. Med. (0022-1007) 2000 tnr6_mouse Tumor necrosis factor receptor superfamily member 6 Fas Apt1 Tnfrsf6 FASLG receptor Apoptosis-mediating surface antigen FAS Apo-1 antigen protein protein mouse Mus musculus Mouse MLWIWAVLPLVLAGSQLRVHTQGTNSISESLKLRRRVRETDKNCSEGLYQGGPFCCQPCQPGKKKVEDCKMNGGTPTCAPCTEGKEYMDKNHYADKCRRCTLCDEEHGLEVETNCTLTQNTKCKCKPDFYCDSPGCEHCVRCASCEHGTLEPCTATSNTNCRKQSPRNRLWLLTILVLLIPLVFIYRKYRKRKCWKRRQDDPESRTSSRETIPMNASNLSLSKYIPRIAEDMTIQEAKKFARENNIKEGKIDEIMHDSIQDTAEQKVQLLLCWYQSHGKSDAYQDLIKGLKKAECRRTLDKFQDMVQKDLGKSTPDTGNENEGQCLE hipk3_mouse Homeodomain-interacting protein kinase 3 Fas-interacting serine/threonine-protein kinase Nuclear body-associated kinase 3 Androgen receptor-interacting nuclear protein kinase Hipk3 Fist3 protein protein mouse Mus musculus Mouse MASQVLVYPPYVYQTQSSAFCSVKKLKVEPSGCVFQERTYPQIHVNGRNFGNSHPSTKGSAFQTKIPFTKPRGHSFSLQAGAIVVKDTAGATKVLAAQAQQAGVEAPRAVVWRNRLHFLEGPQRCGLKRKSEELENHSGAMQIVDELSILPAMLQTNMGNPVTVVTATTGSKQNCTSGEGDYQLVQHEVLCSMKNTYEVLDFLGRGTFGQVVKCWKRGTNEIVAIKILKNHPSYARQGQIEVSILARLSTENADEYNFVRAYECFQHRNHTCLVFEMLEQNLYDFLKQNKFSPLPLKVIRPVLQQVATALKKLKSLGLIHADLKPENIMLVDPVRQPYRVKVIDFGSASHVSKTVCSTYLQSRYYRAPEIILGLPFCEAIDMWSLGCVIAELFLGWPLYPGALEYDQIRYISQTQGLPGEQLLNVGTKSTRFFCRETDMSHSGWRLKTLEEHEAETGMKSKEARKYIFNSLDDIVHVNTVMDLEGGDLLAEKADRREFVNLLKKMLLIDADLRITPIETLNHPFVNMKHLLDFPHSNHVKSCFHIMDICKSPSSCETNNHSKMSLLRPVASNGTAALAANFTKVGTLRSQALTTSAHSVVHHGIPLQAGTAQFGCGDAFHQTLIICPPAIQGIPAAHGKPTSYSIRVDNTVPLVTQAPAVQPLQIRPGVLSQQTWSGRTQQMLIPAWQQVTPMAPAAATLTSEGMAGSQRLGDWGKMIPHSNHYNSVMPPPLLTNQITLSAPQPISVGIAHVVWPQPATTKKNKLCQNRSNSLQNTNIPHSAFISPKIISGKEVEEVSCVDTQDNHTSEGEAGTCREASVRQDSSVSDKQRQTIIIADSPSPAVSVITISSDSDDEETSPRPSLRECKGSLDCEACQSTLNIDRMCSLSSPDSTLSTSSSGQSSPSPCKRPNSMSDDEQESGCETVDGSPTSDSSGHDSPFAENSFVEDAHQNTELGTCAGPEAKPAVGTAVEPPVGRESGLSVDEHMANTDSTCQPLRKGQPAPGKLHQPPALGARQQKPAAAFPQQHLNLSQVQHFGTGHQEWNGNFGHRRQQAYIPTSVTSNPFTLSHGSPNHTAVHAHLAGSTHLGGQPTLLPYPSSASLSSAAPVAHLLASPCTSRPMLQHPTYNISHPSGIVHQVPVGINPRLLPSPTIHQTQYKPIFPPHSYIAASPAYTGFPLSPTKLSQYPYM fadd_mouse FAS-associated death domain protein FAS-associating death domain-containing protein Mediator of receptor induced toxicity Fadd Mort1 protein protein mouse Mus musculus Mouse MDPFLVLLHSLSGSLSGNDLMELKFLCRERVSKRKLERVQSGLDLFTVLLEQNDLERGHTGLLRELLASLRRHDLLQRLDDFEAGTATAAPPGEADLQVAFDIVCDNVGRDWKRLARELKVSEAKMDGIEEKYPRSLSERVRESLKVWKNAEKKNASVAGLVKALRTCRLNLVADLVEEAQESVSKSENMSPVLRDSTVSSSETP daxx_mouse Death domain-associated protein 6 Daxx Daxx protein protein mouse Mus musculus Mouse MATDDSIIVLDDDDEDEAAAQPGPSNLPPNPASTGPGPGLSQQATGLSEPRVDGGSSNSGSRKCYKLDNEKLFEEFLELCKTETSDHPEVVPFLHKLQQRAQSVFLASAEFCNILSRVLARSRKRPAKIYVYINELCTVLKAHSIKKKLNLAPAASTTSEASGPNPPTEPPSDLTNTENTASEASRTRGSRRQIQRLEQLLALYVAEIRRLQEKELDLSELDDPDSSYLQEARLKRKLIRLFGRLCELKDCSSLTGRVIEQRIPYRGTRYPEVNRRIERLINKPGLDTFPDYGDVLRAVEKAATRHSLGLPRQQLQLLAQDAFRDVGVRLQERRHLDLIYNFGCHLTDDYRPGVDPALSDPTLARRLRENRTLAMNRLDEVISKYAMMQDKTEEGERQKRRARLLGTAPQPSDPPQASSESGEGPSGMASQECPTTSKAETDDDDDDDDDDDEDNEESEEEEEEEEEEKEATEDEDEDLEQLQEDQGGDEEEEGGDNEGNESPTSPSDFFHRRNSEPAEGLRTPEGQQKRGLTETPASPPGASLDPPSTDAESSGEQLLEPLLGDESPVSQLAELEMEALPEERDISSPRKKSEDSLPTILENGAAVVTSTSVNGRVSSHTWRDASPPSKRFRKEKKQLGSGLLGNSYIKEPMAQQDSGQNTSVQPMPSPPLASVASVADSSTRVDSPSHELVTSSLCSPSPSLLLQTPQAQSLRQCIYKTSVATQCDPEEIIVLSDSD ubc9_mouse SUMO-conjugating enzyme UBC9 Ube2i Ubc9 Ubce9 Ubce2i SUMO-protein ligase Ubiquitin-conjugating enzyme E2 I Ubiquitin-protein ligase I Ubiquitin carrier protein I Ubiquitin carrier protein 9 RING-type E3 SUMO transferase UBC9 protein protein mouse Mus musculus Mouse MSGIALSRLAQERKAWRKDHPFGFVAVPTKNPDGTMNLMNWECAIPGKKGTPWEGGLFKLRMLFKDDYPSSPPKCKFEPPLFHPNVYPSGTVCLSILEEDKDWRPAITIKQILLGIQELLNEPNIQDPAQAEAYTIYCQNRVEYEKRVRAQAKKFAPS fas-fist Coimmunoprecipitation reveals interaction between the tumor necrosis factor receptor superfamily member 6 precursor (Fas) protein and the Homeodomain-interacting protein kinase 3 (FIST) protein 175530 N/A 175533 unspecified role unspecified role prey prey N/A 175534 unspecified role unspecified role bait bait c terminal binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false n terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false physical association physical association false false false fist-fadd Coimmunoprecipitation reveals interaction between the homeodomain-interacting protein kinase 3 (FIST) protein and the FADD protein 175530 N/A 175534 unspecified role unspecified role bait bait n terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false N/A 175535 unspecified role unspecified role prey prey region preferential binding phosres phosphorylated residue PhosRes Phospho Phosphorylation Phosphorylation (O of Serine, Threonine, Tyrosine and Aspartate, N epsilon of Lysine) undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false The interaction between FIST and FADD was reduced if Fas (tumor necrosis factor receptor superfamily member 6 precursor protein) was not present. fist-daxx Coimmunoprecipitation reveals interaction between the homeodomain-interacting protein kinase 3 (FIST) protein and the Death domain-associated protein 6 protein (Daxx) 175530 N/A 175536 unspecified role unspecified role prey prey N/A 175534 unspecified role unspecified role bait bait n terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false n terminal binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false FIST protein which had a truncated kinase domain (142- 433) displayed reduced binding affinity for Daxx in this study. ubc9-fas Yeast-2-hybrid reveals interaction between SUMO-E2 ligase UBC9 protein and the tumor necrosis factor receptor superfamily member 6 precursor (Fas) protein 175531 N/A 175537 unspecified role unspecified role prey prey N/A 175533 unspecified role unspecified role bait bait cytoplasmic domain cytoplasmic domain binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false fist-fas Yeast-2-hybrid reveals interaction between the homeodomain-interacting protein kinase 3 (FIST) protein and the tumor necrosis factor receptor superfamily member 6 precursor (Fas) protein 175531 N/A 175533 unspecified role unspecified role bait bait cytoplasmic domain cytoplasmic domain binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false N/A 175534 unspecified role unspecified role prey prey region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false fist-fas-1 Directed yeast-2-hybrid reveals interaction between the homeodomain-interacting protein kinase 3 (FIST) protein and the tumor necrosis factor receptor superfamily member 6 precursor (Fas) protein 175532 N/A 175534 unspecified role unspecified role prey prey N/A 175533 unspecified role unspecified role bait bait region binding region binding-associated region binding region deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false