IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ cleyrat-2014-1 Mpl traffics to the cell surface through conventional and unconventional routes. human-hel Homo sapiens HEL erythroleukemia cell line hel human erythroleukemia established from the peripheral blood of a 30-year-old man with erythroleukemia (AML M6) in relapse (after treatment for Hodgkin's leukemia) anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Cleyrat C., Darehshouri A., Steinkamp M.P., Vilaine M., Boassa D., Ellisman M.H., Hermouet S., Wilson B.S. Affinomics - Interactions curated for the Affinomics consortium. The authors confirm that all transfectants are of Human origin. bwilson@salud.unm.edu Traffic (1398-9219) 2014 Only protein-protein interactions imex curation cleyrat-2014-2 Mpl traffics to the cell surface through conventional and unconventional routes. human-k_562 Homo sapiens myeloid leukemia cells. k562 Myelogenous leukemia K562 cells anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Cleyrat C., Darehshouri A., Steinkamp M.P., Vilaine M., Boassa D., Ellisman M.H., Hermouet S., Wilson B.S. Affinomics - Interactions curated for the Affinomics consortium. The authors confirm that all transfectants are of Human origin. bwilson@salud.unm.edu Traffic (1398-9219) 2014 Only protein-protein interactions imex curation cleyrat-2014-3 Mpl traffics to the cell surface through conventional and unconventional routes. human-k_562 Homo sapiens myeloid leukemia cells. k562 Myelogenous leukemia K562 cells confocal microscopy confocal microscopy immunocytochemistry immunocytochemistry Cleyrat C., Darehshouri A., Steinkamp M.P., Vilaine M., Boassa D., Ellisman M.H., Hermouet S., Wilson B.S. Affinomics - Interactions curated for the Affinomics consortium. The authors confirm that all transfectants are of Human origin. bwilson@salud.unm.edu Traffic (1398-9219) 2014 Only protein-protein interactions imex curation cleyrat-2014-4 Mpl traffics to the cell surface through conventional and unconventional routes. human-k_562 Homo sapiens myeloid leukemia cells. k562 Myelogenous leukemia K562 cells confocal microscopy confocal microscopy tag fluorescence tag visualisation by fluorescence tag fluorescence Cleyrat C., Darehshouri A., Steinkamp M.P., Vilaine M., Boassa D., Ellisman M.H., Hermouet S., Wilson B.S. Affinomics - Interactions curated for the Affinomics consortium. The authors confirm that all transfectants are of Human origin. bwilson@salud.unm.edu Traffic (1398-9219) 2014 Only protein-protein interactions imex curation cleyrat-2014-5 Mpl traffics to the cell surface through conventional and unconventional routes. human-hel Homo sapiens HEL erythroleukemia cell line hel human erythroleukemia established from the peripheral blood of a 30-year-old man with erythroleukemia (AML M6) in relapse (after treatment for Hodgkin's leukemia) pla proximity ligation assay pELISA pELISA PLA antibody detection identification by antibody antibody detection Cleyrat C., Darehshouri A., Steinkamp M.P., Vilaine M., Boassa D., Ellisman M.H., Hermouet S., Wilson B.S. Affinomics - Interactions curated for the Affinomics consortium. The authors confirm that all transfectants are of Human origin. bwilson@salud.unm.edu Traffic (1398-9219) 2014 Only protein-protein interactions imex curation cleyrat-2014-6 Mpl traffics to the cell surface through conventional and unconventional routes. human-k_562 Homo sapiens myeloid leukemia cells. k562 Myelogenous leukemia K562 cells pla proximity ligation assay pELISA pELISA PLA antibody detection identification by antibody antibody detection Cleyrat C., Darehshouri A., Steinkamp M.P., Vilaine M., Boassa D., Ellisman M.H., Hermouet S., Wilson B.S. Affinomics - Interactions curated for the Affinomics consortium. The authors confirm that all transfectants are of Human origin. bwilson@salud.unm.edu Traffic (1398-9219) 2014 Only protein-protein interactions imex curation jak2_human Tyrosine-protein kinase JAK2 Janus kinase 2 JAK2 protein protein human Homo sapiens Human MGMACLTMTEMEGTSTSSIYQNGDISGNANSMKQIDPVLQVYLYHSLGKSEADYLTFPSGEYVAEEICIAASKACGITPVYHNMFALMSETERIWYPPNHVFHIDESTRHNVLYRIRFYFPRWYCSGSNRAYRHGISRGAEAPLLDDFVMSYLFAQWRHDFVHGWIKVPVTHETQEECLGMAVLDMMRIAKENDQTPLAIYNSISYKTFLPKCIRAKIQDYHILTRKRIRYRFRRFIQQFSQCKATARNLKLKYLINLETLQSAFYTEKFEVKEPGSGPSGEEIFATIIITGNGGIQWSRGKHKESETLTEQDLQLYCDFPNIIDVSIKQANQEGSNESRVVTIHKQDGKNLEIELSSLREALSFVSLIDGYYRLTADAHHYLCKEVAPPAVLENIQSNCHGPISMDFAISKLKKAGNQTGLYVLRCSPKDFNKYFLTFAVERENVIEYKHCLITKNENEEYNLSGTKKNFSSLKDLLNCYQMETVRSDNIIFQFTKCCPPKPKDKSNLLVFRTNGVSDVPTSPTLQRPTHMNQMVFHKIRNEDLIFNESLGQGTFTKIFKGVRREVGDYGQLHETEVLLKVLDKAHRNYSESFFEAASMMSKLSHKHLVLNYGVCVCGDENILVQEFVKFGSLDTYLKKNKNCINILWKLEVAKQLAWAMHFLEENTLIHGNVCAKNILLIREEDRKTGNPPFIKLSDPGISITVLPKDILQERIPWVPPECIENPKNLNLATDKWSFGTTLWEICSGGDKPLSALDSQRKLQFYEDRHQLPAPKWAELANLINNCMDYEPDFRPSFRAIIRDLNSLFTPDYELLTENDMLPNMRIGALGFSGAFEDRDPTQFEERHLKFLQQLGKGNFGSVEMCRYDPLQDNTGEVVAVKKLQHSTEEHLRDFEREIEILKSLQHDNIVKYKGVCYSAGRRNLKLIMEYLPYGSLRDYLQKHKERIDHIKLLQYTSQICKGMEYLGTKRYIHRDLATRNILVENENRVKIGDFGLTKVLPQDKEYYKVKEPGESPIFWYAPESLTESKFSVASDVWSFGVVLYELFTYIEKSKSPPAEFMRMIGNDKQGQMIVFHLIELLKNNGRLPRPDGCPDEIYMIMTECWNNNVNQRPSFRDLALRVDQIRDNMAG tpor_human Thrombopoietin receptor MPL TPOR Myeloproliferative leukemia protein Proto-oncogene c-Mpl protein protein human Homo sapiens Human MPSWALFMVTSCLLLAPQNLAQVSSQDVSLLASDSEPLKCFSRTFEDLTCFWDEEEAAPSGTYQLLYAYPREKPRACPLSSQSMPHFGTRYVCQFPDQEEVRLFFPLHLWVKNVFLNQTRTQRVLFVDSVGLPAPPSIIKAMGGSQPGELQISWEEPAPEISDFLRYELRYGPRDPKNSTGPTVIQLIATETCCPALQRPHSASALDQSPCAQPTMPWQDGPKQTSPSREASALTAEGGSCLISGLQPGNSYWLQLRSEPDGISLGGSWGSWSLPVTVDLPGDAVALGLQCFTLDLKNVTCQWQQQDHASSQGFFYHSRARCCPRDRYPIWENCEEEEKTNPGLQTPQFSRCHFKSRNDSIIHILVEVTTAPGTVHSYLGSPFWIHQAVRLPTPNLHWREISSGHLELEWQHPSSWAAQETCYQLRYTGEGHQDWKVLEPPLGARGGTLELRPRSRYRLQLRARLNGPTYQGPWSSWSDPTRVETATETAWISLVTALHLVLGLSAVLGLLLLRWQFPAHYRRLRHALWPSLPDLHRVLGQYLRDTAALSPPKATVSDTCEEVEPSLLEILPKSSERTPLPLCSSQAQMDYRRLQPSCLGTMPLSVCPPMAESGSCCTTHIANHSYLPLSYWQQP mpl-jak2 1779017 n/a 1779023 unspecified role unspecified role prey prey O60674:p.Val617Phe mutation mutation certain certain sequence position certain certain certain sequence position certain false n/a 1779024 unspecified role unspecified role bait bait physical association physical association false false false 1F mpl-jak2-1 1779018 n/a 1779023 unspecified role unspecified role prey prey mk02_kusabira_orange fluorescent prot tag fluorescent protein tag fluorescent prot tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1779024 unspecified role unspecified role bait bait physical association physical association false false false 1F jak2-mpl-1 1779019 n/a 1779023 unspecified role unspecified role neutral component neutral component n/a 1779024 tag fluorescence tag visualisation by fluorescence tag fluorescence unspecified role unspecified role neutral component neutral component m_orange fluorescent prot tag fluorescent protein tag fluorescent prot tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false colocalization colocalization false false false 2D jak2-mpl-2 1779020 n/a 1779023 unspecified role unspecified role neutral component neutral component m_citrine fluorescent prot tag fluorescent protein tag fluorescent prot tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false O60674:p.Val617Phe mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false n/a 1779024 unspecified role unspecified role neutral component neutral component m_orange fluorescent prot tag fluorescent protein tag fluorescent prot tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false colocalization colocalization false false false 2 F/G jak2-mpl-3 1779021 n/a 1779023 unspecified role unspecified role neutral component neutral component O60674:p.Val617Phe mutation mutation certain certain sequence position certain certain certain sequence position certain false n/a 1779024 unspecified role unspecified role neutral component neutral component proximity proximity close-contact colocalization neighbourhood interaction false false false 3 C/D association was low jak2-mpl-4 1779022 n/a 1779024 unspecified role unspecified role neutral component neutral component mk02_kusabira_orange fluorescent prot tag fluorescent protein tag fluorescent prot tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1779023 unspecified role unspecified role neutral component neutral component proximity proximity close-contact colocalization neighbourhood interaction false false false 3 E/F association was low