IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ monteiro-2013-3 Coordinated function of WASH and exocyst complex underlies the mechanism of invadopodial matrix degradation by tumor cells human-mda_mb_231 Homo sapiens Caucasian breast adenocarcinoma mda_mb_231 Human Caucasian breast adenocarcinoma fluorescence imaging fluorescence microscopy fluorescence imaging immunocytochemistry immunocytochemistry Monteiro P, Chavrier P, Formstecher E Affinomics - Interactions curated for the Affinomics consortium. philippe.chavrier@curie.fr, pedro.monteiro@curie.fr, eformstecher@hybrigenics.com 2013 Only protein-protein interactions imex curation monteiro-2013-1 Coordinated function of WASH and exocyst complex underlies the mechanism of invadopodial matrix degradation by tumor cells yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2h fragment pooling two hybrid fragment pooling approach 2h fragment pooling nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Monteiro P, Chavrier P, Formstecher E Affinomics - Interactions curated for the Affinomics consortium. Confidence values - A,B and C correspond to interactions found in two reciprocal and independent screens or in a single screen with many overlapping prey fragments. D corresponds to data defined by single bait-singleton fragment interaction-this catogory would include false positives and low representation of the fragments in library. C corresponds to nonspecifically found prey fragments.E corresponds to interactions that may be classified as sticky. philippe.chavrier@curie.fr, pedro.monteiro@curie.fr, eformstecher@hybrigenics.com Human placenta library 2013 Only protein-protein interactions imex curation monteiro-2013-6 Coordinated function of WASH and exocyst complex underlies the mechanism of invadopodial matrix degradation by tumor cells human-mda_mb_231 Homo sapiens Caucasian breast adenocarcinoma mda_mb_231 Human Caucasian breast adenocarcinoma pla proximity ligation assay pELISA pELISA PLA antibody detection identification by antibody antibody detection Monteiro P, Chavrier P, Formstecher E Affinomics - Interactions curated for the Affinomics consortium. philippe.chavrier@curie.fr, pedro.monteiro@curie.fr, eformstecher@hybrigenics.com 2013 Only protein-protein interactions imex curation monteiro-2013-7 Coordinated function of WASH and exocyst complex underlies the mechanism of invadopodial matrix degradation by tumor cells human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Monteiro P, Chavrier P, Formstecher E Affinomics - Interactions curated for the Affinomics consortium. philippe.chavrier@curie.fr, pedro.monteiro@curie.fr, eformstecher@hybrigenics.com 2013 Only protein-protein interactions imex curation monteiro-2013-2 Coordinated function of WASH and exocyst complex underlies the mechanism of invadopodial matrix degradation by tumor cells human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Monteiro P, Chavrier P, Formstecher E Affinomics - Interactions curated for the Affinomics consortium. philippe.chavrier@curie.fr, pedro.monteiro@curie.fr, eformstecher@hybrigenics.com 2013 Only protein-protein interactions imex curation monteiro-2013-4 Coordinated function of WASH and exocyst complex underlies the mechanism of invadopodial matrix degradation by tumor cells human-mda_mb_231 Homo sapiens Caucasian breast adenocarcinoma mda_mb_231 Human Caucasian breast adenocarcinoma confocal microscopy confocal microscopy tag fluorescence tag visualisation by fluorescence tag fluorescence Monteiro P, Chavrier P, Formstecher E Affinomics - Interactions curated for the Affinomics consortium. philippe.chavrier@curie.fr, pedro.monteiro@curie.fr, eformstecher@hybrigenics.com 2013 Only protein-protein interactions imex curation monteiro-2013-5 Coordinated function of WASH and exocyst complex underlies the mechanism of invadopodial matrix degradation by tumor cells human-bt_549 Homo sapiens BT_549 Breast ductal carcinoma cell line bt_549 BT 549 Human Breast ductal carcinoma cell line fluorescence imaging fluorescence microscopy fluorescence imaging immunocytochemistry immunocytochemistry Monteiro P, Chavrier P, Formstecher E Affinomics - Interactions curated for the Affinomics consortium. philippe.chavrier@curie.fr, pedro.monteiro@curie.fr, eformstecher@hybrigenics.com 2013 Only protein-protein interactions imex curation src8_human Src substrate cortactin CTTN EMS1 Amplaxin Oncogene EMS1 protein protein human Homo sapiens Human MWKASAGHAVSIAQDDAGADDWETDPDFVNDVSEKEQRWGAKTVQGSGHQEHINIHKLRENVFQEHQTLKEKELETGPKASHGYGGKFGVEQDRMDKSAVGHEYQSKLSKHCSQVDSVRGFGGKFGVQMDRVDQSAVGFEYQGKTEKHASQKDYSSGFGGKYGVQADRVDKSAVGFDYQGKTEKHESQRDYSKGFGGKYGIDKDKVDKSAVGFEYQGKTEKHESQKDYVKGFGGKFGVQTDRQDKCALGWDHQEKLQLHESQKDYKTGFGGKFGVQSERQDSAAVGFDYKEKLAKHESQQDYSKGFGGKYGVQKDRMDKNASTFEDVTQVSSAYQKTVPVEAVTSKTSNIRANFENLAKEKEQEDRRKAEAERAQRMAKERQEQEEARRKLEEQARAKTQTPPVSPAPQPTEERLPSSPVYEDAASFKAELSYRGPVSGTEPEPVYSMEAADYREASSQQGLAYATEAVYESAEAPGHYPAEDSTYDEYENDLGITAVALYDYQAAGDDEISFDPDDIITNIEMIDDGWWRGVCKGRYGLFPANYVELRQ wash1_human WASH complex subunit 1 WASHC1 CXYorf1-like protein on chromosome 9 FAM39E Protein FAM39E WAS protein family homolog 1 WASH1 protein protein human Homo sapiens Human MTPVRMQHSLAGQTYAVPFIQPDLRREEAVQQMADALQYLQKVSGDIFSRISQQVEQSRSQVQAIGEKVSLAQAKIEKIKGSKKAIKVFSSAKYPAPGRLQEYGSIFTGAQDPGLQRRPRHRIQSKHRPLDERALQEKLKDFPVCVSTKPEPEDDAEEGLGGLPSNISSVSSLLLFNTTENLYKKYVFLDPLAGAVTKTHVMLGAETEEKLFDAPLSISKREQLEQQVPENYFYVPDLGQVPEIHVPSYLPDLPGIANDLMYSADLGPGIAPSAPGTIPELPTFHTEVAEPLKVDLQDGVLTPPPPPPPPPPAPEVLASAPPLPPSTAAPVGQGARQDDSSSSASPSVQGAPREVVDPSGGWATLLESIRQAGGIGKAKLRSMKERKLEKQQQKEQEQVRATSQGGHLMSDLFNKLVMRRKGISGKGPGAGEGPGGAFVRVSDSIPPLPPPQQPQAEEDEDDWES exoc8_human Exocyst complex component 8 Exocyst complex 84 kDa subunit EXOC8 protein protein human Homo sapiens Human MAMAMSDSGASRLRRQLESGGFEARLYVKQLSQQSDGDRDLQEHRQRIQALAEETAQNLKRNVYQNYRQFIETAREISYLESEMYQLSHLLTEQKSSLESIPLTLLPAAAAAGAAAASGGEEGVGGAGGRDHLRGQAGFFSTPGGASRDGSGPGEEGKQRTLTTLLEKVEGCRHLLETPGQYLVYNGDLVEYDADHMAQLQRVHGFLMNDCLLVATWLPQRRGMYRYNALYSLDGLAVVNVKDNPPMKDMFKLLMFPESRIFQAENAKIKREWLEVLEDTKRALSEKRRREQEEAAAPRGPPQVTSKATNPFEDDEEEEPAVPEVEEEKVDLSMEWIQELPEDLDVCIAQRDFEGAVDLLDKLNHYLEDKPSPPPVKELRAKVEERVRQLTEVLVFELSPDRSLRGGPKATRRAVSQLIRLGQCTKACELFLRNRAAAVHTAIRQLRIEGATLLYIHKLCHVFFTSLLETAREFEIDFAGTDSGCYSAFVVWARSAMGMFVDAFSKQVFDSKESLSTAAECVKVAKEHCQQLGDIGLDLTFIIHALLVKDIQGALHSYKEIIIEATKHRNSEEMWRRMNLMTPEALGKLKEEMKSCGVSNFEQYTGDDCWVNLSYTVVAFTKQTMGFLEEALKLYFPELHMVLLESLVEIILVAVQHVDYSLRCEQDPEKKAFIRQNASFLYETVLPVVEKRFEEGVGKPAKQLQDLRNASRLIRVNPESTTSVV wash1_mouse WASH complex subunit 1 Washc1 Orf19 WAS protein family homolog 1 Wash Wash1 protein protein mouse Mus musculus Mouse MTPVKTQCSLAGQLYAVPLIQPDLRREEAIQQVADALQYLQNISGDIFSRISRRVELSRRQLQAISERVSLAQAKIEKIKGSKKAIKVFSSAKYPAPEHLQEYGSIFTGALDPGLQRRPRYRIQSKHRPLDERALQEKLKYFPVCVNTKSEPEDEAEEGLGGLPSNISSISSLLLFNTTENLYKKYVFLDPLAGAVTKTHTMLGTEEEKLFDAPLSISKREQLERQAPENYFYVPDLGQVPEIDVPSYLPDLPGVADDLMYSADLGPGIAPSAPGAIPELPAFHTEVAEPLQPELENEVLLAAPPPPPPPPPPPPPAPTALVSTPQPPMFPDMATAAGQVAREEDSSSSMAHTASVQGAPKEVVDPSSGRATLLESIRQAGGIGKAKLRSVKERKLEKKKQKEQEQVRATSQGGDLMSDLFNKLVMRRKGISGKGPSTGTSEGPGGAFSRMSDSIPPLPPPQQPAGDEDEEDWES exoc1_human Exocyst complex component 1 Exocyst complex component Sec3 EXOC1 SEC3 SEC3L1 BM-012 protein protein human Homo sapiens Human MTAIKHALQRDIFTPNDERLLSIVNVCKAGKKKKNCFLCATVTTERPVQVKVVKVKKSDKGDFYKRQIAWALRDLAVVDAKDAIKENPEFDLHFEKIYKWVASSTAEKNAFISCIWKLNQRYLRKKIDFVNVSSQLLEESVPSGENQSVTGGDEEVVDEYQELNAREEQDIEIMMEGCEYAISNAEAFAEKLSRELQVLDGANIQSIMASEKQVNILMKLLDEALKEVDQIELKLSSYEEMLQSVKEQMDQISESNHLIHLSNTNNVKLLSEIEFLVNHMDLAKGHIKALQEGDLASSRGIEACTNAADALLQCMNVALRPGHDLLLAVKQQQQRFSDLRELFARRLASHLNNVFVQQGHDQSSTLAQHSVELTLPNHHPFHRDLLRYAKLMEWLKSTDYGKYEGLTKNYMDYLSRLYEREIKDFFEVAKIKMTGTTKESKKFATLPRKESAVKQETESLHGSSGKLTGSTSSLNKLSVQSSGNRRSQSSSLLDMGNMSASDLDVADRTKFDKIFEQVLSELEPLCLAEQDFISKFFKLQQHQSMPGTMAEAEDLDGGTLSRQHNCGTPLPVSSEKDMIRQMMIKIFRCIEPELNNLIALGDKIDSFNSLYMLVKMSHHVWTAQNVDPASFLSTTLGNVLVTVKRNFDKCISNQIRQMEEVKISKKSKVGILPFVAEFEEFAGLAESIFKNAERRGDLDKAYTKLIRGVFVNVEKVANESQKTPRDVVMMENFHHIFATLSRLKISCLEAEKKEAKQKYTDHLQSYVIYSLGQPLEKLNHFFEGVEARVAQGIREEEVSYQLAFNKQELRKVIKEYPGKEVKKGLDNLYKKVDKHLCEEENLLQVVWHSMQDEFIRQYKHFEGLIARCYPGSGVTMEFTIQDILDYCSSIAQSH exoc4_human Exocyst complex component 4 EXOC4 KIAA1699 SEC8 SEC8L1 Exocyst complex component Sec8 protein protein human Homo sapiens Human MAAEAAGGKYRSTVSKSKDPSGLLISVIRTLSTSDDVEDRENEKGRLEEAYEKCDRDLDELIVQHYTELTTAIRTYQSITERITNSRNKIKQVKENLLSCKMLLHCKRDELRKLWIEGIEHKHVLNLLDEIENIKQVPQKLEQCMASKHYLSATDMLVSAVESLEGPLLQVEGLSDLRLELHSKKMNLHLVLIDELHRHLYIKSTSRVVQRNKEKGKISSLVKDASVPLIDVTNLPTPRKFLDTSHYSTAGSSSVREINLQDIKEDLELDPEENSTLFMGILIKGLAKLKKIPETVKAIIERLEQELKQIVKRSTTQVADSGYQRGENVTVENQPRLLLELLELLFDKFNAVAAAHSVVLGYLQDTVVTPLTQQEDIKLYDMADVWVKIQDVLQMLLTEYLDMKNTRTASEPSAQLSYASTGREFAAFFAKKKPQRPKNSLFKFESSSHAISMSAYLREQRRELYSRSGELQGGPDDNLIEGGGTKFVCKPGARNITVIFHPLLRFIQEIEHALGLGPAKQCPLREFLTVYIKNIFLNQVLAEINKEIEGVTKTSDPLKILANADTMKVLGVQRPLLQSTIIVEKTVQDLLNLMHDLSAYSDQFLNMVCVKLQEYKDTCTAAYRGIVQSEEKLVISASWAKDDDISRLLKSLPNWMNMAQPKQLRPKREEEEDFIRAAFGKESEVLIGNLGDKLIPPQDILRDVSDLKALANMHESLEWLASRTKSAFSNLSTSQMLSPAQDSHTNTDLPPVSEQIMQTLSELAKSFQDMADRCLLVLHLEVRVHCFHYLIPLAKEGNYAIVANVESMDYDPLVVKLNKDISAIEEAMSASLQQHKFQYIFEGLGHLISCILINGAQYFRRISESGIKKMCRNIFVLQQNLTNITMSREADLDFARQYYEMLYNTADELLNLVVDQGVKYTELEYIHALTLLHRSQTGVGELTTQNTRLQRLKEIICEQAAIKQATKDKKITTV src8_rat Src substrate cortactin Cttn protein protein rat Rattus norvegicus (Rat) Rat MWKASAGHAVSITQDDGGADDWETDPDFVNDVSEKEQRWGAKTVQGSGHQEHINIHKLRENVFQEHQTLKEKELETGPKASHGYGGKFGVEQDRMDKSAVGHEYQSKLSKHCSQVDSVRGFGGKFGVQMDRVDQSAVGFEYQGKTEKHASQKDYSSGFGGKYGVQADRVDKSAVGFDYQGKTEKHESQKDYSKGFGGKYGIDKDKVDKSAVGFEYQGKTEKHESQKDYVKGFGGKFGVQTDRQDKCALGWDHQEKLQLHESQKDYAKGFGGKYGVQKDRMDKNASTFEEVVQVPSAYQKTVPIEAVTSKTSNIRANFENLAKEREQEDRRKAEAERAQRMAQERQEQEEARRKLEEQARAKKQTPPASPSPQPAEDRPPSSPIYEDAAPLKAEPSYGSSEPEPEYSTEAAGLPEASNQQGLAYTSEPVYETTEVPGHYQAEDDTYDGYESDLGITAIALYDYQAAGDDEISFDPDDVITNIEMIDDGWWRGVCKGRYGLFPANYVELRQ cttn-wash1 1299125 n/a 1299132 unspecified role unspecified role neutral component neutral component n/a 1299133 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false 1A/B, 2 D/F Silencing of WASH led to a large reduction of cortactin accumulation on endosomal compartments, whereas silencing of Exo84 was accompanied by a limited but significant increase of the size of WASH patches on MT1-MMP–positive endosomes and a marked lengthening of comet-shaped cortactin domains associated with WASH patches cttn-wash1-1 1299125 n/a 1299132 unspecified role unspecified role neutral component neutral component n/a 1299134 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false Supp 1B, Supp 3B exoc8-wash1-1 1299125 n/a 1299134 unspecified role unspecified role neutral component neutral component n/a 1299135 tag fluorescence tag visualisation by fluorescence tag fluorescence unspecified role unspecified role neutral component neutral component n_terminus egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false colocalization colocalization false false false 1C/D exoc1-wash1 1299126 n/a 1299136 unspecified role unspecified role bait bait n/a 1299133 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false author score author-based confidence author score A score generated by an author, usually only relevant for that particular dataset. A no figure A exoc8-wash1 1299126 n/a 1299134 unspecified role unspecified role bait bait n/a 1299133 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false author score author-based confidence author score A score generated by an author, usually only relevant for that particular dataset. B no figure B exoc8-wash1-3 1299127 n/a 1299133 unspecified role unspecified role neutral component neutral component n/a 1299134 unspecified role unspecified role neutral component neutral component proximity proximity close-contact colocalization neighbourhood interaction false false false 1F/G exoc8-wash1-4 1299127 n/a 1299132 unspecified role unspecified role neutral component neutral component n/a 1299133 unspecified role unspecified role neutral component neutral component proximity proximity close-contact colocalization neighbourhood interaction false false false 1F/Gi wash1-exoc1-1 1299128 n/a 1299133 unspecified role unspecified role bait bait n/a 1299136 unspecified role unspecified role prey prey c_terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false physical association physical association false false false wash1-exoc8-1 1299128 n/a 1299133 unspecified role unspecified role bait bait n/a 1299134 unspecified role unspecified role prey prey c_terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false physical association physical association false false false wash1-exoc4 1299129 n/a 1299137 unspecified role unspecified role prey prey n/a 1299133 unspecified role unspecified role bait bait physical association physical association false false false exoc8-wash1-2 1299130 n/a 1299135 unspecified role unspecified role neutral component neutral component n_terminus egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false n/a 1299138 unspecified role unspecified role neutral component neutral component c_terminus rfp tag red fluorescent protein tag red fluorescent protein RFP red fluorescent protein RFP rfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false colocalization colocalization false false false Video 1, (video 7), figure 7D. cttn-wash1-2 1299131 n/a 1299133 unspecified role unspecified role neutral component neutral component n/a 1299132 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false Supp 1E