HPIDb Host Pathogen Interaction database http://www.agbase.msstate.edu/hpi/main.html http://www.agbase.msstate.edu/hpi/main.html sakata-2013-3 HCV NS3 protease enhances liver fibrosis via binding to and activating TGF-β type I receptor. in vitro In vitro anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Sakata K., Hara M., Terada T., Watanabe N., Takaya D., Yaguchi S., Matsumoto T., Matsuura T., Shirouzu M., Yokoyama S., Yamaguchi T., Miyazawa K., Aizaki H., Suzuki T., Wakita T., Imoto M., Kojima S. Affinomics - Interactions curated for the Affinomics consortium. skojima@riken. jp skojima@riken.jp Sci Rep 2013 imex curation Virus - Publications including interactions involving viral proteins Only protein-protein interactions imex curation sakata-2013-5 HCV NS3 protease enhances liver fibrosis via binding to and activating TGF-β type I receptor. in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Sakata K., Hara M., Terada T., Watanabe N., Takaya D., Yaguchi S., Matsumoto T., Matsuura T., Shirouzu M., Yokoyama S., Yamaguchi T., Miyazawa K., Aizaki H., Suzuki T., Wakita T., Imoto M., Kojima S. Affinomics - Interactions curated for the Affinomics consortium. skojima@riken. jp skojima@riken.jp Sci Rep 2013 imex curation Virus - Publications including interactions involving viral proteins Only protein-protein interactions imex curation sakata-2013-1 HCV NS3 protease enhances liver fibrosis via binding to and activating TGF-β type I receptor. human-huh-7.5 Human Huh-7.5 cells huh75 Huh-7.5 cells confocal microscopy confocal microscopy immunostaining immunostaining Sakata K., Hara M., Terada T., Watanabe N., Takaya D., Yaguchi S., Matsumoto T., Matsuura T., Shirouzu M., Yokoyama S., Yamaguchi T., Miyazawa K., Aizaki H., Suzuki T., Wakita T., Imoto M., Kojima S. Affinomics - Interactions curated for the Affinomics consortium. skojima@riken. jp skojima@riken.jp Sci Rep 2013 imex curation Virus - Publications including interactions involving viral proteins Only protein-protein interactions imex curation sakata-2013-2 HCV NS3 protease enhances liver fibrosis via binding to and activating TGF-β type I receptor. human-huh-7.5 Human Huh-7.5 cells huh75 Huh-7.5 cells pla proximity ligation assay pELISA pELISA PLA immunostaining immunostaining Sakata K., Hara M., Terada T., Watanabe N., Takaya D., Yaguchi S., Matsumoto T., Matsuura T., Shirouzu M., Yokoyama S., Yamaguchi T., Miyazawa K., Aizaki H., Suzuki T., Wakita T., Imoto M., Kojima S. Affinomics - Interactions curated for the Affinomics consortium. skojima@riken. jp skojima@riken.jp Sci Rep 2013 imex curation Virus - Publications including interactions involving viral proteins Only protein-protein interactions imex curation q99ib8-pro_0000045599 Serine protease/helicase NS3 protein protein hcvjf Hepatitis C virus genotype 2a (isolate JFH-1) HCV APITAYAQQTRGLLGAIVVSMTGRDRTEQAGEVQILSTVSQSFLGTTISGVLWTVYHGAGNKTLAGLRGPVTQMYSSAEGDLVGWPSPPGTKSLEPCKCGAVDLYLVTRNADVIPARRRGDKRGALLSPRPISTLKGSSGGPVLCPRGHVVGLFRAAVCSRGVAKSIDFIPVETLDVVTRSPTFSDNSTPPAVPQTYQVGYLHAPTGSGKSTKVPVAYAAQGYKVLVLNPSVAATLGFGAYLSKAHGINPNIRTGVRTVMTGEAITYSTYGKFLADGGCASGAYDIIICDECHAVDATSILGIGTVLDQAETAGVRLTVLATATPPGSVTTPHPDIEEVGLGREGEIPFYGRAIPLSCIKGGRHLIFCHSKKKCDELAAALRGMGLNAVAYYRGLDVSIIPAQGDVVVVATDALMTGYTGDFDSVIDCNVAVTQAVDFSLDPTFTITTQTVPQDAVSRSQRRGRTGRGRQGTYRYVSTGERASGMFDSVVLCECYDAGAAWYDLTPAETTVRLRAYFNTPGLPVCQDHLEFWEAVFTGLTHIDAHFLSQTKQAGENFAYLVAYQATVCARAKAPPPSWDAMWKCLARLKPTLAGPTPLLYRLGPITNEVTLTHPGTKYIATCMQADLEVMT 1031 1661 tgfr1_human TGF-beta receptor type-1 TGFBR1 Transforming growth factor-beta receptor type I TGF-beta type I receptor Serine/threonine-protein kinase receptor R4 Activin receptor-like kinase 5 ALK5 SKR4 Activin A receptor type II-like protein kinase of 53kD protein protein human Homo sapiens Human MEAAVAAPRPRLLLLVLAAAAAAAAALLPGATALQCFCHLCTKDNFTCVTDGLCFVSVTETTDKVIHNSMCIAEIDLIPRDRPFVCAPSSKTGSVTTTYCCNQDHCNKIELPTTVKSSPGLGPVELAAVIAGPVCFVCISLMLMVYICHNRTVIHHRVPNEEDPSLDRPFISEGTTLKDLIYDMTTSGSGSGLPLLVQRTIARTIVLQESIGKGRFGEVWRGKWRGEEVAVKIFSSREERSWFREAEIYQTVMLRHENILGFIAADNKDNGTWTQLWLVSDYHEHGSLFDYLNRYTVTVEGMIKLALSTASGLAHLHMEIVGTQGKPAIAHRDLKSKNILVKKNGTCCIADLGLAVRHDSATDTIDIAPNHRVGTKRYMAPEVLDDSINMKHFESFKRADIYAMGLVFWEIARRCSIGGIHEDYQLPYYDLVPSDPSVEEMRKVVCEQKLRPNIPNRWQSCEALRVMAKIMRECWYANGAARLTALRIKKTLSQLSQQEGIKM tgfr2_human TGF-beta receptor type-2 TGFBR2 Transforming growth factor-beta receptor type II TGF-beta type II receptor protein protein human Homo sapiens Human MGRGLLRGLWPLHIVLWTRIASTIPPHVQKSVNNDMIVTDNNGAVKFPQLCKFCDVRFSTCDNQKSCMSNCSITSICEKPQEVCVAVWRKNDENITLETVCHDPKLPYHDFILEDAASPKCIMKEKKKPGETFFMCSCSSDECNDNIIFSEEYNTSNPDLLLVIFQVTGISLLPPLGVAISVIIIFYCYRVNRQQKLSSTWETGKTRKLMEFSEHCAIILEDDRSDISSTCANNINHNTELLPIELDTLVGKGRFAEVYKAKLKQNTSEQFETVAVKIFPYEEYASWKTEKDIFSDINLKHENILQFLTAEERKTELGKQYWLITAFHAKGNLQEYLTRHVISWEDLRKLGSSLARGIAHLHSDHTPCGRPKMPIVHRDLKSSNILVKNDLTCCLCDFGLSLRLDPTLSVDDLANSGQVGTARYMAPEVLESRMNLENVESFKQTDVYSMALVLWEMTSRCNAVGEVKDYEPPFGSKVREHPCVESMKDNVLRDRGRPEIPSFWLNHQGIQMVCETLTECWDHDPEARLTAQCVAERFSELEHLDRLSGRSCSEEKIPEDGSLNTTK ns3-tbr1-3 1469264 n/a 1469268 unspecified role unspecified role bait bait flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) n/a 1469269 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false Figure 3D and supp. data S5 ns3-tbr2-1 1469264 n/a 1469270 unspecified role unspecified role prey prey his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) n/a 1469268 unspecified role unspecified role bait bait flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false Figure 3D and supp. data S5 ns3-tbr1-4 1469265 n/a 1469268 unspecified role unspecified role prey prey flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) n/a 1469269 unspecified role unspecified role bait bait gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false direct interaction direct interaction false false false Supp. data S7A tgfbr1-ns3-competitor-1 1469265 n/a 1469268 competitor competitor competitor prey prey gly120-ser139 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false chemical synthesis Chemical synthesis (Chemical synthesis) n/a 1469268 unspecified role unspecified role prey prey flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) n/a 1469269 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Supp. data S7A ns3-tbr1-2 1469266 n/a 1469269 unspecified role unspecified role neutral component neutral component n/a 1469268 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false Figure 3B ns3-tbr1-1 1469267 n/a 1469268 unspecified role unspecified role neutral component neutral component n/a 1469269 unspecified role unspecified role neutral component neutral component proximity proximity close-contact colocalization neighbourhood interaction false false false Figure 3C