IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ guvenc-2013-2 Impairment of glioma stem cell survival and growth by a novel inhibitor for Survivin-Ran protein complex. in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Guvenc H., Pavlyukov M.S., Joshi K., Kurt H., Banasavadi-Siddegowda Y.K., Mao P., Hong C., Yamada R., Kwon C.H., Bhasin D., Chettiar S., Kitange G., Park I.H., Sarkaria J.N., Li C., Shakhparonov M.I., Nakano I. Cancer - Interactions investigated in the context of cancer Survivin has been entered as Human based on References 26, 27 (PDB entry), 31 (Blat of primers used), 32 and 33. SMAC/DIABLO has been entered as Human based on reference 31 (Blat of primers). [The stated reference (30) - Pistollato F et al., for "His-tagged Smac/DAIBLO, Survivin WT and Survivin F101A/L102A were expressed and purified as described previously." does not mention any of these molecules. ]. Ichiro.Nakano@osumc.edu Clin. Cancer Res. (1078-0432) 2013 Only protein-protein interactions imex curation Affinomics - Interactions curated for the Affinomics consortium. guvenc-2013-3 Impairment of glioma stem cell survival and growth by a novel inhibitor for Survivin-Ran protein complex. in vitro In vitro elisa enzyme linked immunosorbent assay ELISA ELISA elisa antibody detection identification by antibody antibody detection Guvenc H., Pavlyukov M.S., Joshi K., Kurt H., Banasavadi-Siddegowda Y.K., Mao P., Hong C., Yamada R., Kwon C.H., Bhasin D., Chettiar S., Kitange G., Park I.H., Sarkaria J.N., Li C., Shakhparonov M.I., Nakano I. Cancer - Interactions investigated in the context of cancer Survivin has been entered as Human based on References 26, 27 (PDB entry), 31 (Blat of primers used), 32 and 33. SMAC/DIABLO has been entered as Human based on reference 31 (Blat of primers). [The stated reference (30) - Pistollato F et al., for "His-tagged Smac/DAIBLO, Survivin WT and Survivin F101A/L102A were expressed and purified as described previously." does not mention any of these molecules. ]. Ichiro.Nakano@osumc.edu Clin. Cancer Res. (1078-0432) 2013 Only protein-protein interactions imex curation Affinomics - Interactions curated for the Affinomics consortium. guvenc-2013-4 Impairment of glioma stem cell survival and growth by a novel inhibitor for Survivin-Ran protein complex. human-u87 Human U-87 MG glioblastoma cell line u87 pla proximity ligation assay pELISA pELISA PLA antibody detection identification by antibody antibody detection Guvenc H., Pavlyukov M.S., Joshi K., Kurt H., Banasavadi-Siddegowda Y.K., Mao P., Hong C., Yamada R., Kwon C.H., Bhasin D., Chettiar S., Kitange G., Park I.H., Sarkaria J.N., Li C., Shakhparonov M.I., Nakano I. Cancer - Interactions investigated in the context of cancer Survivin has been entered as Human based on References 26, 27 (PDB entry), 31 (Blat of primers used), 32 and 33. SMAC/DIABLO has been entered as Human based on reference 31 (Blat of primers). [The stated reference (30) - Pistollato F et al., for "His-tagged Smac/DAIBLO, Survivin WT and Survivin F101A/L102A were expressed and purified as described previously." does not mention any of these molecules. ]. Ichiro.Nakano@osumc.edu Clin. Cancer Res. (1078-0432) 2013 Only protein-protein interactions imex curation Affinomics - Interactions curated for the Affinomics consortium. guvenc-2013-1 Impairment of glioma stem cell survival and growth by a novel inhibitor for Survivin-Ran protein complex. in vitro In vitro fluorescence spectr classical fluorescence spectroscopy fluorescence spectr predetermined predetermined participant predetermined Guvenc H., Pavlyukov M.S., Joshi K., Kurt H., Banasavadi-Siddegowda Y.K., Mao P., Hong C., Yamada R., Kwon C.H., Bhasin D., Chettiar S., Kitange G., Park I.H., Sarkaria J.N., Li C., Shakhparonov M.I., Nakano I. Cancer - Interactions investigated in the context of cancer Survivin has been entered as Human based on References 26, 27 (PDB entry), 31 (Blat of primers used), 32 and 33. SMAC/DIABLO has been entered as Human based on reference 31 (Blat of primers). [The stated reference (30) - Pistollato F et al., for "His-tagged Smac/DAIBLO, Survivin WT and Survivin F101A/L102A were expressed and purified as described previously." does not mention any of these molecules. ]. Ichiro.Nakano@osumc.edu Clin. Cancer Res. (1078-0432) 2013 Only protein-protein interactions imex curation Affinomics - Interactions curated for the Affinomics consortium. guvenc-2013-5 Impairment of glioma stem cell survival and growth by a novel inhibitor for Survivin-Ran protein complex. human-glioblastoma Homo sapiens glioblastoma glioblastoma Glioblastoma Glioblastoma is the same as 'Glioblastoma multiforme' (GBM) -is the same as Astrocytoma Grade 4. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Guvenc H., Pavlyukov M.S., Joshi K., Kurt H., Banasavadi-Siddegowda Y.K., Mao P., Hong C., Yamada R., Kwon C.H., Bhasin D., Chettiar S., Kitange G., Park I.H., Sarkaria J.N., Li C., Shakhparonov M.I., Nakano I. Cancer - Interactions investigated in the context of cancer Lysed GBM83 sphere cells:- patient-derived glioblastoma cells grown as spheres that are enriched with Glioma Stem Cells. Survivin has been entered as Human based on References 26, 27 (PDB entry), 31 (Blat of primers used), 32 and 33. SMAC/DIABLO has been entered as Human based on reference 31 (Blat of primers). [The stated reference (30) - Pistollato F et al., for "His-tagged Smac/DAIBLO, Survivin WT and Survivin F101A/L102A were expressed and purified as described previously." does not mention any of these molecules. ]. Ichiro.Nakano@osumc.edu Clin. Cancer Res. (1078-0432) 2013 Only protein-protein interactions imex curation Affinomics - Interactions curated for the Affinomics consortium. birc5_human Baculoviral IAP repeat-containing protein 5 Apoptosis inhibitor survivin Apoptosis inhibitor 4 BIRC5 API4 IAP4 protein protein human Homo sapiens Human MGAPTLPPAWQPFLKDHRISTFKNWPFLEGCACTPERMAEAGFIHCPTENEPDLAQCFFCFKELEGWEPDDDPIEEHKKHSSGCAFLSVKKQFEELTLGEFLKLDRERAKNKIAKETNNKKKEFEETAKKVRRAIEQLAAMD llp-3 LLP-3 small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) dbloh_human Diablo homolog, mitochondrial Second mitochondria-derived activator of caspase Direct IAP-binding protein with low pI DIABLO SMAC protein protein human Homo sapiens Human MAALKSWLSRSVTSFFRYRQCLCVPVVANFKKRCFSELIRPWHKTVTIGFGVTLCAVPIAQKSEPHSLSSEALMRRAVSLVTDSTSTFLSQTTYALIEAITEYTKAVYTLTSLYRQYTSLLGKMNSEEEDEVWQVIIGARAEMTSKHQEYLKLETTWMTAVGLSEMAAEAAYQTGADQASITARNHIQLVKLQVEEVHQLSRKAETKLAEAQIEELRQKTQEEGEERAESEQEAYLRED ran_human GTP-binding nuclear protein Ran GTPase Ran Ras-related nuclear protein Ras-like protein TC4 Androgen receptor-associated protein 24 RAN ARA24 OK/SW-cl.81 protein protein human Homo sapiens Human MAAQGEPQVQFKLVLVGDGGTGKTTFVKRHLTGEFEKKYVATLGVEVHPLVFHTNRGPIKFNVWDTAGQEKFGGLRDGYYIQAQCAIIMFDVTSRVTYKNVPNWHRDLVRVCENIPIVLCGNKVDIKDRKVKAKSIVFHRKKNLQYYDISAKSNYNFEKPFLWLARKLIGDPNLEFVAMPALAPPEVVMDPALAAQYEHDLEVAQTTALPDEDDDL xiap_human E3 ubiquitin-protein ligase XIAP Inhibitor of apoptosis protein 3 X-linked inhibitor of apoptosis protein IAP-like protein XIAP API3 IAP3 BIRC4 Baculoviral IAP repeat-containing protein 4 RING-type E3 ubiquitin transferase XIAP protein protein human Homo sapiens Human MTFNSFEGSKTCVPADINKEEEFVEEFNRLKTFANFPSGSPVSASTLARAGFLYTGEGDTVRCFSCHAAVDRWQYGDSAVGRHRKVSPNCRFINGFYLENSATQSTNSGIQNGQYKVENYLGSRDHFALDRPSETHADYLLRTGQVVDISDTIYPRNPAMYSEEARLKSFQNWPDYAHLTPRELASAGLYYTGIGDQVQCFCCGGKLKNWEPCDRAWSEHRRHFPNCFFVLGRNLNIRSESDAVSSDRNFPNSTNLPRNPSMADYEARIFTFGTWIYSVNKEQLARAGFYALGEGDKVKCFHCGGGLTDWKPSEDPWEQHAKWYPGCKYLLEQKGQEYINNIHLTHSLEECLVRTTEKTPSLTRRIDDTIFQNPMVQEAIRMGFSFKDIKKIMEEKIQISGSNYKSLEVLVADLVNAQKDSMQDESSQTSLQKEISTEEQLRRLQEEKLCKICMDRNIAIVFVPCGHLVTCKQCAEAVDKCPMCYTVITFKQKIFMS diablo-birc5-5 1545666 n/a 1545671 unspecified role unspecified role prey prey region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1545672 inhibitor inhibitor unspecified role unspecified role chemical synthesis Chemical synthesis (Chemical synthesis) n/a 1545673 unspecified role unspecified role bait bait region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false direct interaction direct interaction false false false 4A-1 birc5-diablo 1545666 n/a 1545672 inhibitor inhibitor unspecified role unspecified role chemical synthesis Chemical synthesis (Chemical synthesis) n/a 1545671 unspecified role unspecified role bait bait region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1545674 unspecified role unspecified role prey prey region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false direct interaction direct interaction false false false 4A-3 diablo-birc5-4 1545666 n/a 1545673 unspecified role unspecified role bait bait region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1545671 unspecified role unspecified role prey prey region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false direct interaction direct interaction false false false 4A-1 diablo-birc5-3 1545666 n/a 1545675 unspecified role unspecified role prey prey bir2_and3_domains sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1545673 unspecified role unspecified role bait bait region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false direct interaction direct interaction false false false 4A-2 birc5-ran 1545666 n/a 1545671 unspecified role unspecified role bait bait region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1545674 unspecified role unspecified role prey prey region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false direct interaction direct interaction false false false 4A-3i birc5-ran-1 1545666 n/a 1545671 unspecified role unspecified role bait bait region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1545671 unspecified role unspecified role prey prey region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false direct interaction direct interaction false false false 4A-5 birc5-ran-2 1545667 n/a 1545671 unspecified role unspecified role bait bait region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1545674 unspecified role unspecified role prey prey region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false direct interaction direct interaction false false false 4B birc5-ran-3 1545667 n/a 1545674 unspecified role unspecified role prey prey region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1545671 unspecified role unspecified role bait bait region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1545672 inhibitor inhibitor unspecified role unspecified role chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction false false false 4B birc5-llp_3-1 1545668 n/a 1545671 unspecified role unspecified role neutral component neutral component n/a 1545674 unspecified role unspecified role neutral component neutral component n/a 1545672 inhibitor inhibitor neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) proximity proximity close-contact colocalization neighbourhood interaction false false false 4C birc5-ran-6 1545668 n/a 1545674 unspecified role unspecified role neutral component neutral component n/a 1545671 unspecified role unspecified role neutral component neutral component proximity proximity close-contact colocalization neighbourhood interaction false false false 4C birc5-llp_3 1545669 n/a 1545671 unspecified role unspecified role neutral component neutral component O15392:p.[Phe101Ala;Leu102Ala] mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) n/a 1545672 unspecified role unspecified role neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction false false false 3C birc5-ran-5 1545670 n/a 1545674 unspecified role unspecified role prey prey n/a 1545671 unspecified role unspecified role bait bait physical association physical association false false false 5A