IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ lifland-2012-4 Human respiratory syncytial virus nucleoprotein and inclusion bodies antagonize the innate immune response mediated by MDA5 and MAVS. chlae-vero Chlorocebus (Cercopithecus) aethiops kidney cells Grivet vero Chlorocebus (Cercopithecus) aethiops kidney cells similar to cos-7 cells but these are not trasnformed pla proximity ligation assay pELISA pELISA PLA antibody detection identification by antibody antibody detection Lifland A.W., Jung J., Alonas E., Zurla C., Crowe J.E., Santangelo P.J. Virus - Publications including interactions involving viral proteins Colocalizations that were not confirmed by additional interaction evidence were not curated. Interactions involving MAD5 constructs were not put in because the origin of the constructs could nto be ascertained. The authors were contacted but there was no reply. philip.santangelo@bme.gatech.edu J. Virol. (0022-538X) 2012 Only protein-protein interactions imex curation Affinomics - Interactions curated for the Affinomics consortium. lifland-2012-3 Human respiratory syncytial virus nucleoprotein and inclusion bodies antagonize the innate immune response mediated by MDA5 and MAVS. chlae-vero Chlorocebus (Cercopithecus) aethiops kidney cells Grivet vero Chlorocebus (Cercopithecus) aethiops kidney cells similar to cos-7 cells but these are not trasnformed confocal microscopy confocal microscopy immunostaining immunostaining Lifland A.W., Jung J., Alonas E., Zurla C., Crowe J.E., Santangelo P.J. Virus - Publications including interactions involving viral proteins Colocalizations that were not confirmed by additional interaction evidence were not curated. Interactions involving MAD5 constructs were not put in because the origin of the constructs could nto be ascertained. The authors were contacted but there was no reply. philip.santangelo@bme.gatech.edu J. Virol. (0022-538X) 2012 Only protein-protein interactions imex curation Affinomics - Interactions curated for the Affinomics consortium. lifland-2012-1 Human respiratory syncytial virus nucleoprotein and inclusion bodies antagonize the innate immune response mediated by MDA5 and MAVS. human-a_549 Homo sapiens Caucasian lung carcinoma cell line a_549 Human Caucasian lung carcinoma confocal microscopy confocal microscopy immunostaining immunostaining Lifland A.W., Jung J., Alonas E., Zurla C., Crowe J.E., Santangelo P.J. Virus - Publications including interactions involving viral proteins Colocalizations that were not confirmed by additional interaction evidence were not curated. Interactions involving MAD5 constructs were not put in because the origin of the constructs could nto be ascertained. The authors were contacted but there was no reply. philip.santangelo@bme.gatech.edu J. Virol. (0022-538X) 2012 Only protein-protein interactions imex curation Affinomics - Interactions curated for the Affinomics consortium. lifland-2012-2 Human respiratory syncytial virus nucleoprotein and inclusion bodies antagonize the innate immune response mediated by MDA5 and MAVS. human-hep2 Homo sapiens hep2 hep2 confocal microscopy confocal microscopy immunostaining immunostaining Lifland A.W., Jung J., Alonas E., Zurla C., Crowe J.E., Santangelo P.J. Virus - Publications including interactions involving viral proteins Colocalizations that were not confirmed by additional interaction evidence were not curated. Interactions involving MAD5 constructs were not put in because the origin of the constructs could nto be ascertained. The authors were contacted but there was no reply. philip.santangelo@bme.gatech.edu J. Virol. (0022-538X) 2012 Only protein-protein interactions imex curation Affinomics - Interactions curated for the Affinomics consortium. ncap_hrsva Nucleoprotein N Nucleocapsid protein protein protein hrsva human respiratory sy MALSKVKLNDTLNKDQLLSSSKYTIQRSTGDSIDTPNYDVQKHINKLCGMLLITEDANHKFTGLIGMLYAMSRLGREDTIKILRDAGYHVKANGVDVTTHRQDINGKEMKFEVLTLASLTTEIQINIEIESRKSYKKMLKEMGEVAPEYRHDSPDCGMIILCIAALVITKLAAGDRSGLTAVIRRANNVLKNEMKRYKGLLPKDIANSFYEVFEKHPHFIDVFVHFGIAQSSTRGGSRVEGIFAGLFMNAYGAGQVMLRWGVLAKSVKNIMLGHASVQAEMEQVVEVYEYAQKLGGEAGFYHILNNPKASLLSLTQFPHFSSVVLGNAAGLGIMGEYRGTPRNQDLYDAAKAYAEQLKENGVINYSVLDLTAEELEAIKHQLNPKDNDVEL ifih1_chlae_protein Similar to Interferon-induced helicase C domain-containing protein 1 protein protein chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey Similar to human Q9BYX4. m1qcq2_chlae MAVS protein protein chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey MPFAEDKTYKYICRNFSNFCNVDVVEILPYLPCLTARDQDRLRATCTLSGNRDTLWHLFSTLQRRPGWVESFIAALRDCELADLADEVACVYQSYQPRTSDRPPDPLEPPSLPAEGPGPPTPAAAHSIPYNSYREKEPSYPMPVQETRAPDSPGESSEQAPQILNPRVIPRSPDGGPLDPSSDLAALSPLTSSGHQEQDTELGSTHTAGATSSLTPSRGPVSPSVSFQPLARSTPRASRLPGPAGSVVSTGTSSSSSSSPGLASAGAAEGEQGAESDQAEPIICSSGAEAPANSLPSKVPTTLMPVNTVAPKVPANPASASTVPSKLPTSSKPPGAVPSVFTNPAPSKLPINSTRAGMVPSKVPTSMVRTKVSASTVPTDRSSRTEETPAAPTPAGATGGRSAWLDSSSENGGFESELSKPGMLVSQADSQFSGCSEDLAISASTSLGMGSCHGPEENEYKSEGTFGIHVAENPSIQLMEGNPGPPAGPQGGPRPHVDQKFQKREVPCHRPSPGALWLQAAVAGVLVVTLLVAMYRRRLH phosp_hrsva Phosphoprotein P protein protein hrsva human respiratory sy MEKFAPEFHGEDANNRATKFLESIKGKFTSPKDPKKKDSIISVNSIDIEVTKESPITSNSTIINPTNETDDTAGNKPNYQRKPLVSFKEDPTPSDNPFSKLYKETIETFDNNEEESSYSYEEINDQTNDNITARLDRIDEKLSEILGMLHTLVVASAGPTSARDGIRDAMIGLREEMIEKIRTEALMTNDRLEAMARLRNEESEKMAKDTSDEVSLNPTSEKLNNLLEGNDSDNDLSLEDF mavs_human Mitochondrial antiviral-signaling protein Interferon beta promoter stimulator protein 1 Virus-induced-signaling adapter CARD adapter inducing interferon beta Putative NF-kappa-B-activating protein 031N MAVS IPS1 KIAA1271 VISA protein protein human Homo sapiens Human MPFAEDKTYKYICRNFSNFCNVDVVEILPYLPCLTARDQDRLRATCTLSGNRDTLWHLFNTLQRRPGWVEYFIAALRGCELVDLADEVASVYQSYQPRTSDRPPDPLEPPSLPAERPGPPTPAAAHSIPYNSCREKEPSYPMPVQETQAPESPGENSEQALQTLSPRAIPRNPDGGPLESSSDLAALSPLTSSGHQEQDTELGSTHTAGATSSLTPSRGPVSPSVSFQPLARSTPRASRLPGPTGSVVSTGTSFSSSSPGLASAGAAEGKQGAESDQAEPIICSSGAEAPANSLPSKVPTTLMPVNTVALKVPANPASVSTVPSKLPTSSKPPGAVPSNALTNPAPSKLPINSTRAGMVPSKVPTSMVLTKVSASTVPTDGSSRNEETPAAPTPAGATGGSSAWLDSSSENRGLGSELSKPGVLASQVDSPFSGCFEDLAISASTSLGMGPCHGPEENEYKSEGTFGIHVAENPSIQLLEGNPGPPADPDGGPRPQADRKFQEREVPCHRPSPGALWLQVAVTGVLVVTLLVVLYRRRLH ifih1_human Interferon-induced helicase C domain-containing protein 1 Helicase with 2 CARD domains Interferon-induced with helicase C domain protein 1 Melanoma differentiation-associated protein 5 Murabutide down-regulated protein RNA helicase-DEAD box protein 116 RIG-I-like receptor 2 IFIH1 MDA5 RH116 Clinically amyopathic dermatomyositis autoantigen 140 kDa protein protein human Homo sapiens Human MSNGYSTDENFRYLISCFRARVKMYIQVEPVLDYLTFLPAEVKEQIQRTVATSGNMQAVELLLSTLEKGVWHLGWTREFVEALRRTGSPLAARYMNPELTDLPSPSFENAHDEYLQLLNLLQPTLVDKLLVRDVLDKCMEEELLTIEDRNRIAAAENNGNESGVRELLKRIVQKENWFSAFLNVLRQTGNNELVQELTGSDCSESNAEIENLSQVDGPQVEEQLLSTTVQPNLEKEVWGMENNSSESSFADSSVVSESDTSLAEGSVSCLDESLGHNSNMGSDSGTMGSDSDEENVAARASPEPELQLRPYQMEVAQPALEGKNIIICLPTGSGKTRVAVYIAKDHLDKKKKASEPGKVIVLVNKVLLVEQLFRKEFQPFLKKWYRVIGLSGDTQLKISFPEVVKSCDIIISTAQILENSLLNLENGEDAGVQLSDFSLIIIDECHHTNKEAVYNNIMRHYLMQKLKNNRLKKENKPVIPLPQILGLTASPGVGGATKQAKAEEHILKLCANLDAFTIKTVKENLDQLKNQIQEPCKKFAIADATREDPFKEKLLEIMTRIQTYCQMSPMSDFGTQPYEQWAIQMEKKAAKEGNRKERVCAEHLRKYNEALQINDTIRMIDAYTHLETFYNEEKDKKFAVIEDDSDEGGDDEYCDGDEDEDDLKKPLKLDETDRFLMTLFFENNKMLKRLAENPEYENEKLTKLRNTIMEQYTRTEESARGIIFTKTRQSAYALSQWITENEKFAEVGVKAHHLIGAGHSSEFKPMTQNEQKEVISKFRTGKINLLIATTVAEEGLDIKECNIVIRYGLVTNEIAMVQARGRARADESTYVLVAHSGSGVIEHETVNDFREKMMYKAIHCVQNMKPEEYAHKILELQMQSIMEKKMKTKRNIAKHYKNNPSLITFLCKNCSVLACSGEDIHVIEKMHHVNMTPEFKELYIVRENKALQKKCADYQINGEIICKCGQAWGTMMVHKGLDLPCLKIRNFVVVFKNNSTKKQYKKWVELPITFPNLDYSECCLFSDED ddx58_human Antiviral innate immune response receptor RIG-I DEAD box protein 58 Retinoic acid-inducible gene 1 protein DDX58 Probable ATP-dependent RNA helicase DDX58 Retinoic acid-inducible gene I protein RIG-I-like receptor 1 protein protein human Homo sapiens Human MTTEQRRSLQAFQDYIRKTLDPTYILSYMAPWFREEEVQYIQAEKNNKGPMEAATLFLKFLLELQEEGWFRGFLDALDHAGYSGLYEAIESWDFKKIEKLEEYRLLLKRLQPEFKTRIIPTDIISDLSECLINQECEEILQICSTKGMMAGAEKLVECLLRSDKENWPKTLKLALEKERNKFSELWIVEKGIKDVETEDLEDKMETSDIQIFYQEDPECQNLSENSCPPSEVSDTNLYSPFKPRNYQLELALPAMKGKNTIICAPTGCGKTFVSLLICEHHLKKFPQGQKGKVVFFANQIPVYEQQKSVFSKYFERHGYRVTGISGATAENVPVEQIVENNDIIILTPQILVNNLKKGTIPSLSIFTLMIFDECHNTSKQHPYNMIMFNYLDQKLGGSSGPLPQVIGLTASVGVGDAKNTDEALDYICKLCASLDASVIATVKHNLEELEQVVYKPQKFFRKVESRISDKFKYIIAQLMRDTESLAKRICKDLENLSQIQNREFGTQKYEQWIVTVQKACMVFQMPDKDEESRICKALFLYTSHLRKYNDALIISEHARMKDALDYLKDFFSNVRAAGFDEIEQDLTQRFEEKLQELESVSRDPSNENPKLEDLCFILQEEYHLNPETITILFVKTRALVDALKNWIEGNPKLSFLKPGILTGRGKTNQNTGMTLPAQKCILDAFKASGDHNILIATSVADEGIDIAQCNLVILYEYVGNVIKMIQTRGRGRARGSKCFLLTSNAGVIEKEQINMYKEKMMNDSILRLQTWDEAVFREKILHIQTHEKFIRDSQEKPKPVPDKENKKLLCRKCKALACYTADVRVIEECHYTVLGDAFKECFVSRPHPKPKQFSSFEKRAKIFCARQNCSHDWGIHVKYKTFEIPVIKIESFVVEDIATGVQTLYSKWKDFHFEKIPFDPAEMSK ifih1_chlae_prot-n-1 1434566 n/a 1434570 unspecified role unspecified role neutral component neutral component n/a 1434571 unspecified role unspecified role neutral component neutral component proximity proximity close-contact colocalization neighbourhood interaction false false false 7 Interaction found in viral inclusion bodies induced upon RSV infection. mavs-n-3 1434566 n/a 1434572 unspecified role unspecified role neutral component neutral component n/a 1434570 unspecified role unspecified role neutral component neutral component proximity proximity close-contact colocalization neighbourhood interaction false false false 7 Interaction found in viral inclusion bodies induced upon RSV infection. ifih1_chlae_prot-n-2 1434566 n/a 1434570 unspecified role unspecified role neutral component neutral component region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1434571 unspecified role unspecified role neutral component neutral component proximity proximity close-contact colocalization neighbourhood interaction false false false 9 If the cells are co-transfected with RSV P, the interaction is found in inclusion bodies. mavs-n-3 1434566 n/a 1434570 unspecified role unspecified role neutral component neutral component region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1434572 unspecified role unspecified role neutral component neutral component proximity proximity close-contact colocalization neighbourhood interaction false false false 9 If the cells are co-transfected with RSV P, the interaction is found in inclusion bodies. mda5-n-3 1434566 n/a 1434573 unspecified role unspecified role neutral component neutral component region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1434570 unspecified role unspecified role ancillary ancillary n/a 1434571 unspecified role unspecified role neutral component neutral component proximity proximity close-contact colocalization neighbourhood interaction false false false 13 Interaction found in inclusion bodies. ifih1_chlae_protei-n 1434567 n/a 1434570 unspecified role unspecified role neutral component neutral component region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1434571 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false 5 The cell were co-transfected with RSV P. mavs-n-3 1434567 n/a 1434570 unspecified role unspecified role neutral component neutral component region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1434572 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false 5 The cell were co-transfected with RSV P. p-n 1434567 n/a 1434573 unspecified role unspecified role neutral component neutral component n/a 1434570 unspecified role unspecified role neutral component neutral component region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false colocalization colocalization false false false 16 The cell were co-transfected with RSV P. ifih1-mavs 1434568 n/a 1434574 unspecified role unspecified role neutral component neutral component n/a 1434575 unspecified role unspecified role neutral component neutral component n/a 1434570 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false 3 Interaction found in viral inclusion bodies upon RSV infection. ddx58-mavs-7 1434568 n/a 1434576 unspecified role unspecified role neutral component neutral component n/a 1434574 unspecified role unspecified role neutral component neutral component n/a 1434570 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false 2 Interaction found in viral inclusion bodies upon RSV infection. ifih1-n-1 1434569 n/a 1434575 unspecified role unspecified role neutral component neutral component n/a 1434570 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false 4 Interaction found in viral filaments upon RSV infection.