IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ bellucci-2011-1 Redistribution of DAT/alpha-synuclein complexes visualized by "in situ" proximity ligation assay in transgenic mice modelling early Parkinson's disease. human-sh_sy5y Homo sapiens SH-SY5Y cell line sh_sy5y Human neuroblastoma cells pla proximity ligation assay pELISA pELISA PLA antibody detection identification by antibody antibody detection Bellucci A., Navarria L., Falarti E., Zaltieri M., Bono F., Collo G., Spillantini M.G., Grazia M., Missale C., Spano P. Parkinsons - Interactions investigated in the context of Parkinsons disease Co-localizations depicted in figure S3 were not curated because no additional evidence on the interaction between APP and DAT was shown in the publication. bellucci@med.unibs.it PLoS ONE 2011 Only protein-protein interactions imex curation Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Affinomics - Interactions curated for the Affinomics consortium. bellucci-2011-2 Redistribution of DAT/alpha-synuclein complexes visualized by "in situ" proximity ligation assay in transgenic mice modelling early Parkinson's disease. human-sh_sy5y Homo sapiens SH-SY5Y cell line sh_sy5y Human neuroblastoma cells anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Bellucci A., Navarria L., Falarti E., Zaltieri M., Bono F., Collo G., Spillantini M.G., Grazia M., Missale C., Spano P. Parkinsons - Interactions investigated in the context of Parkinsons disease Co-localizations depicted in figure S3 were not curated because no additional evidence on the interaction between APP and DAT was shown in the publication. bellucci@med.unibs.it PLoS ONE 2011 Only protein-protein interactions imex curation Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Affinomics - Interactions curated for the Affinomics consortium. bellucci-2011-3 Redistribution of DAT/alpha-synuclein complexes visualized by "in situ" proximity ligation assay in transgenic mice modelling early Parkinson's disease. mouse-brain-striatum Mouse striatum brain_striatum Brain striatum, Neostratum. fluorescence imaging fluorescence microscopy fluorescence imaging immunostaining immunostaining Bellucci A., Navarria L., Falarti E., Zaltieri M., Bono F., Collo G., Spillantini M.G., Grazia M., Missale C., Spano P. Parkinsons - Interactions investigated in the context of Parkinsons disease Co-localizations depicted in figure S3 were not curated because no additional evidence on the interaction between APP and DAT was shown in the publication. bellucci@med.unibs.it PLoS ONE 2011 Only protein-protein interactions imex curation Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Affinomics - Interactions curated for the Affinomics consortium. bellucci-2011-4 Redistribution of DAT/alpha-synuclein complexes visualized by "in situ" proximity ligation assay in transgenic mice modelling early Parkinson's disease. mouse-brain_substantia nigra Mus musculus, brain, substantia nigra brain_substantia nigra BRAIN_SUBSTANTIA NIGRA fluorescence imaging fluorescence microscopy fluorescence imaging immunostaining immunostaining Bellucci A., Navarria L., Falarti E., Zaltieri M., Bono F., Collo G., Spillantini M.G., Grazia M., Missale C., Spano P. Parkinsons - Interactions investigated in the context of Parkinsons disease Co-localizations depicted in figure S3 were not curated because no additional evidence on the interaction between APP and DAT was shown in the publication. bellucci@med.unibs.it PLoS ONE 2011 Only protein-protein interactions imex curation Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Affinomics - Interactions curated for the Affinomics consortium. bellucci-2011-5 Redistribution of DAT/alpha-synuclein complexes visualized by "in situ" proximity ligation assay in transgenic mice modelling early Parkinson's disease. mouse-brain-striatum Mouse striatum brain_striatum Brain striatum, Neostratum. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Bellucci A., Navarria L., Falarti E., Zaltieri M., Bono F., Collo G., Spillantini M.G., Grazia M., Missale C., Spano P. Parkinsons - Interactions investigated in the context of Parkinsons disease Co-localizations depicted in figure S3 were not curated because no additional evidence on the interaction between APP and DAT was shown in the publication. bellucci@med.unibs.it PLoS ONE 2011 Only protein-protein interactions imex curation Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Affinomics - Interactions curated for the Affinomics consortium. bellucci-2011-6 Redistribution of DAT/alpha-synuclein complexes visualized by "in situ" proximity ligation assay in transgenic mice modelling early Parkinson's disease. mouse-brain-striatum Mouse striatum brain_striatum Brain striatum, Neostratum. pla proximity ligation assay pELISA pELISA PLA antibody detection identification by antibody antibody detection Bellucci A., Navarria L., Falarti E., Zaltieri M., Bono F., Collo G., Spillantini M.G., Grazia M., Missale C., Spano P. Parkinsons - Interactions investigated in the context of Parkinsons disease Co-localizations depicted in figure S3 were not curated because no additional evidence on the interaction between APP and DAT was shown in the publication. bellucci@med.unibs.it PLoS ONE 2011 Only protein-protein interactions imex curation Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Affinomics - Interactions curated for the Affinomics consortium. bellucci-2011-7 Redistribution of DAT/alpha-synuclein complexes visualized by "in situ" proximity ligation assay in transgenic mice modelling early Parkinson's disease. mouse-brain_substantia nigra Mus musculus, brain, substantia nigra brain_substantia nigra BRAIN_SUBSTANTIA NIGRA pla proximity ligation assay pELISA pELISA PLA antibody detection identification by antibody antibody detection Bellucci A., Navarria L., Falarti E., Zaltieri M., Bono F., Collo G., Spillantini M.G., Grazia M., Missale C., Spano P. Parkinsons - Interactions investigated in the context of Parkinsons disease Co-localizations depicted in figure S3 were not curated because no additional evidence on the interaction between APP and DAT was shown in the publication. bellucci@med.unibs.it PLoS ONE 2011 Only protein-protein interactions imex curation Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Affinomics - Interactions curated for the Affinomics consortium. syua_human Alpha-synuclein Non-A beta component of AD amyloid Non-A4 component of amyloid precursor SNCA NACP PARK1 protein protein human Homo sapiens Human MDVFMKGLSKAKEGVVAAAEKTKQGVAEAAGKTKEGVLYVGSKTKEGVVHGVATVAEKTKEQVTNVGGAVVTGVTAVAQKTVEGAGSIAAATGFVKKDQLGKNEEGAPQEGILEDMPVDPDNEAYEMPSEEGYQDYEPEA sc6a3_human Sodium-dependent dopamine transporter SLC6A3 DAT1 Solute carrier family 6 member 3 protein protein human Homo sapiens Human MSKSKCSVGLMSSVVAPAKEPNAVGPKEVELILVKEQNGVQLTSSTLTNPRQSPVEAQDRETWGKKIDFLLSVIGFAVDLANVWRFPYLCYKNGGGAFLVPYLLFMVIAGMPLFYMELALGQFNREGAAGVWKICPILKGVGFTVILISLYVGFFYNVIIAWALHYLFSSFTTELPWIHCNNSWNSPNCSDAHPGDSSGDSSGLNDTFGTTPAAEYFERGVLHLHQSHGIDDLGPPRWQLTACLVLVIVLLYFSLWKGVKTSGKVVWITATMPYVVLTALLLRGVTLPGAIDGIRAYLSVDFYRLCEASVWIDAATQVCFSLGVGFGVLIAFSSYNKFTNNCYRDAIVTTSINSLTSFSSGFVVFSFLGYMAQKHSVPIGDVAKDGPGLIFIIYPEAIATLPLSSAWAVVFFIMLLTLGIDSAMGGMESVITGLIDEFQLLHRHRELFTLFIVLATFLLSLFCVTNGGIYVFTLLDHFAAGTSILFGVLIEAIGVAWFYGVGQFSDDIQQMTGQRPSLYWRLCWKLVSPCFLLFVVVVSIVTFRPPHYGAYIFPDWANALGWVIATSSMAMVPIYAAYKFCSLPGSFREKLAYAIAPEKDRELVDRGEVRQFTLRHWLKV syn1_human Synapsin-1 Synapsin I Brain protein 4.1 SYN1 protein protein human Homo sapiens Human MNYLRRRLSDSNFMANLPNGYMTDLQRPQPPPPPPGAHSPGATPGPGTATAERSSGVAPAASPAAPSPGSSGGGGFFSSLSNAVKQTTAAAAATFSEQVGGGSGGAGRGGAASRVLLVIDEPHTDWAKYFKGKKIHGEIDIKVEQAEFSDLNLVAHANGGFSVDMEVLRNGVKVVRSLKPDFVLIRQHAFSMARNGDYRSLVIGLQYAGIPSVNSLHSVYNFCDKPWVFAQMVRLHKKLGTEEFPLIDQTFYPNHKEMLSSTTYPVVVKMGHAHSGMGKVKVDNQHDFQDIASVVALTKTYATAEPFIDAKYDVRVQKIGQNYKAYMRTSVSGNWKTNTGSAMLEQIAMSDRYKLWVDTCSEIFGGLDICAVEALHGKDGRDHIIEVVGSSMPLIGDHQDEDKQLIVELVVNKMAQALPRQRQRDASPGRGSHGQTPSPGALPLGRQTSQQPAGPPAQQRPPPQGGPPQPGPGPQRQGPPLQQRPPPQGQQHLSGLGPPAGSPLPQRLPSPTSAPQQPASQAAPPTQGQGRQSRPVAGGPGAPPAARPPASPSPQRQAGPPQATRQTSVSGPAPPKASGAPPGGQQRQGPPQKPPGPAGPTRQASQAGPVPRTGPPTTQQPRPSGPGPAGRPKPQLAQKPSQDVPPPATAAAGGPPHPQLNKSQSLTNAFNLPEPAPPRPSLSQDEVKAETIRSLRKSFASLFSD sc6a3_mouse Sodium-dependent dopamine transporter Slc6a3 Dat Dat1 Solute carrier family 6 member 3 protein protein mouse Mus musculus Mouse MSKSKCSVGPMSSVVAPAKEPNAVGPREVELILVKEQNGVQLTNSTLINPPQTPVEVQERETWSKKIDFLLSVIGFAVDLANVWRFPYLCYKNGGGAFLVPYLLFMVIAGMPLFYMELALGQFNREGAAGVWKICPVLKGVGFTVILISFYVGFFYNVIIAWALHYFFSSFTMDLPWIHCNNTWNSPNCSDAHSSNSSDGLGLNDTFGTTPAAEYFERGVLHLHQSRGIDDLGPPRWQLTACLVLVIVLLYFSLWKGVKTSGKVVWITATMPYVVLTALLLRGVTLPGAMDGIRAYLSVDFYRLCEASVWIDAATQVCFSLGVGFGVLIAFSSYNKFTNNCYRDAIITTSINSLTSFSSGFVVFSFLGYMAQKHNVPIRDVATDGPGLIFIIYPEAIATLPLSSAWAAVFFLMLLTLGIDSAMGGMESVITGLVDEFQLLHRHRELFTLGIVLATFLLSLFCVTNGGIYVFTLLDHFAAGTSILFGVLIEAIGVAWFYGVQQFSDDIKQMTGQRPNLYWRLCWKLVSPCFLLYVVVVSIVTFRPPHYGAYIFPDWANALGWIIATSSMAMVPIYATYKFCSLPGSFREKLAYAITPEKDRQLVDRGEVRQFTLRHWLLV Amine transporter. Terminates the action of dopamine by its high affinity sodium-dependent reuptake into presynaptic terminals mint syua_mouse Alpha-synuclein Snca Syn Non-A beta component of AD amyloid Non-A4 component of amyloid precursor protein protein mouse Mus musculus Mouse MDVFMKGLSKAKEGVVAAAEKTKQGVAEAAGKTKEGVLYVGSKTKEGVVHGVTTVAEKTKEQVTNVGGAVVTGVTAVAQKTVEGAGNIAAATGFVKKDQMGKGEEGYPQEGILEDMPVDPGSEAYEMPSEEGYQDYEPEA slc6a3-snca 1435589 n/a 1435596 unspecified role unspecified role neutral component neutral component n/a 1435597 unspecified role unspecified role neutral component neutral component proximity proximity close-contact colocalization neighbourhood interaction false false false 1a-1d Inclusions where the interaction can be detected are formed upon glucose deprivation and with the over-expression of a truncated version of human alpha-synuclein (residues 1-120). syn1-snca-1 1435589 n/a 1435598 unspecified role unspecified role neutral component neutral component n/a 1435596 unspecified role unspecified role neutral component neutral component proximity proximity close-contact colocalization neighbourhood interaction false false false 1e, 1f Inclusions where the interaction can be detected are formed upon over-expression of a truncated version of human alpha-synuclein (residues 1-120). snca-slc6a3 1435590 n/a 1435598 unspecified role unspecified role prey prey n/a 1435597 unspecified role unspecified role prey prey n/a 1435596 unspecified role unspecified role bait bait association association false false false S2b snca-slc6a3-1 1435590 n/a 1435597 unspecified role unspecified role prey prey n/a 1435596 unspecified role unspecified role bait bait physical association physical association false false false S2c slc6a3-snca-1 1435591 n/a 1435599 unspecified role unspecified role neutral component neutral component n/a 1435600 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false 2c This interaction was detected in C57BL/6J mouse, which come from a background in which alpha-syn has been knocked-down and then rescued. slc6a3-snca-2 1435591 n/a 1435599 unspecified role unspecified role neutral component neutral component n/a 1435596 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false colocalization colocalization false false false 2f This interaction was detected in C57BL/6S mouse, a background in which endogenous alpha-syn has been knocked-down. slc6a3-snca-3 1435592 n/a 1435599 unspecified role unspecified role neutral component neutral component n/a 1435596 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false colocalization colocalization false false false 3f This interaction was detected in C57BL/6S mouse, a background in which endogenous alpha-syn has been knocked-down. slc6a3-snca-3 1435592 n/a 1435599 unspecified role unspecified role neutral component neutral component n/a 1435600 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false 3c This interaction was detected in C57BL/6J mouse, which come from a background in which alpha-syn has been knocked-down and then rescued. slc6a3-snca-5 1435593 n/a 1435599 unspecified role unspecified role bait bait n/a 1435600 unspecified role unspecified role prey prey physical association physical association false false false S4 This interaction was detected in C57BL/6J mouse, which come from a background in which alpha-syn has been knocked-down and then rescued. slc6a3-snca-6 1435593 n/a 1435599 unspecified role unspecified role bait bait n/a 1435596 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false S4 This interaction was detected in C57BL/6S mouse, a background in which endogenous alpha-syn has been knocked-down. slc6a3-snca-7 1435594 n/a 1435599 unspecified role unspecified role neutral component neutral component n/a 1435600 unspecified role unspecified role neutral component neutral component proximity proximity close-contact colocalization neighbourhood interaction false false false 4a, 4d This interaction was detected in C57BL/6J mouse, which come from a background in which alpha-syn has been knocked-down and then rescued. slc6a3-snca-7 1435594 n/a 1435599 unspecified role unspecified role neutral component neutral component n/a 1435596 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false proximity proximity close-contact colocalization neighbourhood interaction false false false 4b, 4e This interaction was detected in C57BL/6S mouse, a background in which endogenous alpha-syn has been knocked-down. slc6a3-snca-8 1435595 n/a 1435599 unspecified role unspecified role neutral component neutral component n/a 1435600 unspecified role unspecified role neutral component neutral component proximity proximity close-contact colocalization neighbourhood interaction false false false 5a, 5d This interaction was detected in C57BL/6J mouse, which come from a background in which alpha-syn has been knocked-down and then rescued. slc6a3-snca-8 1435595 n/a 1435599 unspecified role unspecified role neutral component neutral component n/a 1435596 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false proximity proximity close-contact colocalization neighbourhood interaction false false false 5b, 5e This interaction was detected in C57BL/6S mouse, a background in which endogenous alpha-syn has been knocked-down.