IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ lolicato-2011-4 Tetramerization dynamics of C-terminal domain underlies isoform-specific cAMP gating in hyperpolarization-activated cyclic nucleotide-gated channels. in vitro In vitro fps fluorescence polarization spectroscopy Fluorescence anisotropy FPS FPS Fluorescence anisotropy fps predetermined predetermined participant predetermined Lolicato M., Nardini M., Gazzarrini S., Möller S., Bertinetti D., Herberg F.W., Bolognesi M., Martin H., Fasolini M., Bertrand J.A., Arrigoni C., Thiel G., Moroni A. Affinomics - Interactions curated for the Affinomics consortium. anna.moroni@unimi.it J. Biol. Chem. (0021-9258) 2011 Only protein-protein interactions imex curation lolicato-2011-7 Tetramerization dynamics of C-terminal domain underlies isoform-specific cAMP gating in hyperpolarization-activated cyclic nucleotide-gated channels. in vitro In vitro dls dynamic light scattering dls predetermined predetermined participant predetermined Lolicato M., Nardini M., Gazzarrini S., Möller S., Bertinetti D., Herberg F.W., Bolognesi M., Martin H., Fasolini M., Bertrand J.A., Arrigoni C., Thiel G., Moroni A. Affinomics - Interactions curated for the Affinomics consortium. anna.moroni@unimi.it J. Biol. Chem. (0021-9258) 2011 Only protein-protein interactions imex curation lolicato-2011-3 Tetramerization dynamics of C-terminal domain underlies isoform-specific cAMP gating in hyperpolarization-activated cyclic nucleotide-gated channels. in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Lolicato M., Nardini M., Gazzarrini S., Möller S., Bertinetti D., Herberg F.W., Bolognesi M., Martin H., Fasolini M., Bertrand J.A., Arrigoni C., Thiel G., Moroni A. Affinomics - Interactions curated for the Affinomics consortium. anna.moroni@unimi.it J. Biol. Chem. (0021-9258) 2011 Only protein-protein interactions imex curation lolicato-2011-1 Tetramerization dynamics of C-terminal domain underlies isoform-specific cAMP gating in hyperpolarization-activated cyclic nucleotide-gated channels. in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Lolicato M., Nardini M., Gazzarrini S., Möller S., Bertinetti D., Herberg F.W., Bolognesi M., Martin H., Fasolini M., Bertrand J.A., Arrigoni C., Thiel G., Moroni A. Affinomics - Interactions curated for the Affinomics consortium. anna.moroni@unimi.it J. Biol. Chem. (0021-9258) 2011 Only protein-protein interactions imex curation lolicato-2011-2 Tetramerization dynamics of C-terminal domain underlies isoform-specific cAMP gating in hyperpolarization-activated cyclic nucleotide-gated channels. in vitro In vitro solution sedimentati cosedimentation in solution solution sedimentati predetermined predetermined participant predetermined Lolicato M., Nardini M., Gazzarrini S., Möller S., Bertinetti D., Herberg F.W., Bolognesi M., Martin H., Fasolini M., Bertrand J.A., Arrigoni C., Thiel G., Moroni A. Affinomics - Interactions curated for the Affinomics consortium. anna.moroni@unimi.it J. Biol. Chem. (0021-9258) 2011 Only protein-protein interactions imex curation lolicato-2011-5 Tetramerization dynamics of C-terminal domain underlies isoform-specific cAMP gating in hyperpolarization-activated cyclic nucleotide-gated channels. in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Lolicato M., Nardini M., Gazzarrini S., Möller S., Bertinetti D., Herberg F.W., Bolognesi M., Martin H., Fasolini M., Bertrand J.A., Arrigoni C., Thiel G., Moroni A. Affinomics - Interactions curated for the Affinomics consortium. anna.moroni@unimi.it J. Biol. Chem. (0021-9258) 2011 Only protein-protein interactions imex curation lolicato-2011-6 Tetramerization dynamics of C-terminal domain underlies isoform-specific cAMP gating in hyperpolarization-activated cyclic nucleotide-gated channels. in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Lolicato M., Nardini M., Gazzarrini S., Möller S., Bertinetti D., Herberg F.W., Bolognesi M., Martin H., Fasolini M., Bertrand J.A., Arrigoni C., Thiel G., Moroni A. Affinomics - Interactions curated for the Affinomics consortium. anna.moroni@unimi.it J. Biol. Chem. (0021-9258) 2011 Only protein-protein interactions imex curation hcn1_mouse Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1 Hcn1 Bcng1 Hac2 Brain cyclic nucleotide-gated channel 1 Hyperpolarization-activated cation channel 2 protein protein mouse Mus musculus Mouse MEGGGKPNSASNSRDDGNSVFPSKAPATGPVAADKRLGTPPGGGAAGKEHGNSVCFKVDGGGGEEPAGSFEDAEGPRRQYGFMQRQFTSMLQPGVNKFSLRMFGSQKAVEKEQERVKTAGFWIIHPYSDFRFYWDLIMLIMMVGNLVIIPVGITFFTEQTTTPWIIFNVASDTVFLLDLIMNFRTGTVNEDSSEIILDPKVIKMNYLKSWFVVDFISSIPVDYIFLIVEKGMDSEVYKTARALRIVRFTKILSLLRLLRLSRLIRYIHQWEEIFHMTYDLASAVVRIFNLIGMMLLLCHWDGCLQFLVPLLQDFPPDCWVSLNEMVNDSWGKQYSYALFKAMSHMLCIGYGAQAPVSMSDLWITMLSMIVGATCYAMFVGHATALIQSLDSSRRQYQEKYKQVEQYMSFHKLPADMRQKIHDYYEHRYQGKIFDEENILSELNDPLREEIVNFNCRKLVATMPLFANADPNFVTAMLSKLRFEVFQPGDYIIREGAVGKKMYFIQHGVAGVITKSSKEMKLTDGSYFGEICLLTKGRRTASVRADTYCRLYSLSVDNFNEVLEEYPMMRRAFETVAIDRLDRIGKKNSILLQKFQKDLNTGVFNNQENEILKQIVKHDREMVQAIPPINYPQMTALNCTSSTTTPTSRMRTQSPPVYTATSLSHSNLHSPSPSTQTPQPSAILSPCSYTTAVCSPPIQSPLATRTFHYASPTASQLSLMQQPQQQLPQSQVQQTQTQTQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQPQTPGSSTPKNEVHKSTQALHNTNLTKEVRPLSASQPSLPHEVSTLISRPHPTVGESLASIPQPVAAVHSTGLQAGSRSTVPQRVTLFRQMSSGAIPPNRGVPPAPPPPAAVQRESPSVLNTDPDAEKPRFASNL 3',5'-cyclic gmp 3',5'-cyclic GMP 3',5'-Cyclic GMP cGMP Cyclic GMP Guanosine 3',5'-cyclic monophosphate Guanosine 3',5'-cyclic phosphate Guanosine cyclic monophosphate guanosine 3',5'-(hydrogen phosphate) small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) ZOOGRGPOEVQQDX-UUOKFMHZSA-N hcn2_human Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2 HCN2 BCNG2 Brain cyclic nucleotide-gated channel 2 protein protein human Homo sapiens Human MDARGGGGRPGESPGATPAPGPPPPPPPAPPQQQPPPPPPPAPPPGPGPAPPQHPPRAEALPPEAADEGGPRGRLRSRDSSCGRPGTPGAASTAKGSPNGECGRGEPQCSPAGPEGPARGPKVSFSCRGAASGPAPGPGPAEEAGSEEAGPAGEPRGSQASFMQRQFGALLQPGVNKFSLRMFGSQKAVEREQERVKSAGAWIIHPYSDFRFYWDFTMLLFMVGNLIIIPVGITFFKDETTAPWIVFNVVSDTFFLMDLVLNFRTGIVIEDNTEIILDPEKIKKKYLRTWFVVDFVSSIPVDYIFLIVEKGIDSEVYKTARALRIVRFTKILSLLRLLRLSRLIRYIHQWEEIFHMTYDLASAVMRICNLISMMLLLCHWDGCLQFLVPMLQDFPRNCWVSINGMVNHSWSELYSFALFKAMSHMLCIGYGRQAPESMTDIWLTMLSMIVGATCYAMFIGHATALIQSLDSSRRQYQEKYKQVEQYMSFHKLPADFRQKIHDYYEHRYQGKMFDEDSILGELNGPLREEIVNFNCRKLVASMPLFANADPNFVTAMLTKLKFEVFQPGDYIIREGTIGKKMYFIQHGVVSVLTKGNKEMKLSDGSYFGEICLLTRGRRTASVRADTYCRLYSLSVDNFNEVLEEYPMMRRAFETVAIDRLDRIGKKNSILLHKVQHDLNSGVFNNQENAIIQEIVKYDREMVQQAELGQRVGLFPPPPPPPQVTSAIATLQQAAAMSFCPQVARPLVGPLALGSPRLVRRPPPGPAPAAASPGPPPPASPPGAPASPRAPRTSPYGGLPAAPLAGPALPARRLSRASRPLSASQPSLPHGAPGPAASTRPASSSTPRLGPTPAARAAAPSPDRRDSASPGAAGGLDPQDSARSRLSSNL 3',5'-cyclic amp 3',5'-cyclic AMP 3',5'-Cyclic AMP adenosine 3',5'-cyclic monophosphate Adenosine 3',5'-cyclic phosphate Adenosine 3',5'-phosphate ADENOSINE-3',5'-CYCLIC-MONOPHOSPHATE cAMP Cyclic adenylic acid Cyclic AMP adenosine 3',5'-(hydrogen phosphate) small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) hcn4_human Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 4 HCN4 protein protein human Homo sapiens Human MDKLPPSMRKRLYSLPQQVGAKAWIMDEEEDAEEEGAGGRQDPSRRSIRLRPLPSPSPSAAAGGTESRSSALGAADSEGPARGAGKSSTNGDCRRFRGSLASLGSRGGGSGGTGSGSSHGHLHDSAEERRLIAEGDASPGEDRTPPGLAAEPERPGASAQPAASPPPPQQPPQPASASCEQPSVDTAIKVEGGAAAGDQILPEAEVRLGQAGFMQRQFGAMLQPGVNKFSLRMFGSQKAVEREQERVKSAGFWIIHPYSDFRFYWDLTMLLLMVGNLIIIPVGITFFKDENTTPWIVFNVVSDTFFLIDLVLNFRTGIVVEDNTEIILDPQRIKMKYLKSWFMVDFISSIPVDYIFLIVETRIDSEVYKTARALRIVRFTKILSLLRLLRLSRLIRYIHQWEEIFHMTYDLASAVVRIVNLIGMMLLLCHWDGCLQFLVPMLQDFPDDCWVSINNMVNNSWGKQYSYALFKAMSHMLCIGYGRQAPVGMSDVWLTMLSMIVGATCYAMFIGHATALIQSLDSSRRQYQEKYKQVEQYMSFHKLPPDTRQRIHDYYEHRYQGKMFDEESILGELSEPLREEIINFNCRKLVASMPLFANADPNFVTSMLTKLRFEVFQPGDYIIREGTIGKKMYFIQHGVVSVLTKGNKETKLADGSYFGEICLLTRGRRTASVRADTYCRLYSLSVDNFNEVLEEYPMMRRAFETVALDRLDRIGKKNSILLHKVQHDLNSGVFNYQENEIIQQIVQHDREMAHCAHRVQAAASATPTPTPVIWTPLIQAPLQAAAATTSVAIALTHHPRLPAAIFRPPPGSGLGNLGAGQTPRHLKRLQSLIPSALGSASPASSPSQVDTPSSSSFHIQQLAGFSAPAGLSPLLPSSSSSPPPGACGSPSAPTPSAGVAATTIAGFGHFHKALGGSLSSSDSPLLTPLQPGARSPQAAQPSPAPPGARGGLGLPEHFLPPPPSSRSPSSSPGQLGQPPGELSLGLATGPLSTPETPPRQPEPPSLVAGASGGASPVGFTPRGGLSPPGHSPGPPRTFPSAPPRASGSHGSLLLPPASSPPPPQVPQRRGTPPLTPGRLTQDLKLISASQPALPQDGAQTLRRASPHSSGESMAAFPLFPRAGGGSGGSGSSGGLGPPGRPYGAIPGQHVTLPRKTSSGSLPPPLSLFGARATSSGGPPLTAGPQREPGARPEPVRSKLPSNL 3__5__cyclic_-hcn1-1 1440295 n/a 1440302 unspecified role unspecified role neutral component neutral component purified purified c-linker and cnbd domains sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false mbp-his tag tandem tag tandem tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) n/a 1440303 unspecified role unspecified role neutral component neutral component purified purified 8-fluoresceinyl group fluorescein fluorescein label fluorescein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction false false false 1440295 Table 1, 1a 3__5__cyclic_-hcn2-3 1440295 n/a 1440304 unspecified role unspecified role neutral component neutral component purified purified c-linker and cnbd domains sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false mbp-his tag tandem tag tandem tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) n/a 1440303 unspecified role unspecified role neutral component neutral component purified purified 8-fluoresceinyl group fluorescein fluorescein label fluorescein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction false false false 1440295 Table 1, 1b 3__5__cyclic_-hcn1-3 1440295 n/a 1440302 unspecified role unspecified role neutral component neutral component purified purified c-linker and cnbd domains sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) n/a 1440303 unspecified role unspecified role neutral component neutral component purified purified 8-fluoresceinyl group fluorescein fluorescein label fluorescein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction false false false 1440295 Table 1 3__5__cyclic_-hcn2-1 1440295 n/a 1440304 unspecified role unspecified role neutral component neutral component purified purified c-linker and cnbd domains sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false mbp-his tag tandem tag tandem tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) n/a 1440305 unspecified role unspecified role neutral component neutral component purified purified 8-fluoresceinyl group fluorescein fluorescein label fluorescein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction false false false 1440295 Table 1, 1b 3__5__cyclic_-hcn2-1 1440295 n/a 1440303 unspecified role unspecified role neutral component neutral component purified purified 8-fluoresceinyl group fluorescein fluorescein label fluorescein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false chemical synthesis Chemical synthesis (Chemical synthesis) n/a 1440304 unspecified role unspecified role neutral component neutral component purified purified c-linker and cnbd domains sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false 1440295 Table 1 3__5__cyclic_-hcn1-1 1440295 n/a 1440305 unspecified role unspecified role neutral component neutral component purified purified 8-fluoresceinyl group fluorescein fluorescein label fluorescein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false chemical synthesis Chemical synthesis (Chemical synthesis) n/a 1440302 unspecified role unspecified role neutral component neutral component purified purified c-linker and cnbd domains sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false 1440295 Table 1, 1a 3__5__cyclic_-hcn1-3 1440295 n/a 1440302 unspecified role unspecified role neutral component neutral component purified purified c-linker and cnbd domains sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false mbp-his tag tandem tag tandem tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) n/a 1440305 unspecified role unspecified role neutral component neutral component purified purified 8-fluoresceinyl group fluorescein fluorescein label fluorescein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction false false false 1440295 Table 1, 1a 3__5__cyclic_-hcn2-3 1440295 n/a 1440304 unspecified role unspecified role neutral component neutral component purified purified c-linker and cnbd domains sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) n/a 1440305 unspecified role unspecified role neutral component neutral component purified purified 8-fluoresceinyl group fluorescein fluorescein label fluorescein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction false false false 1440295 Table 1 3__5__cyclic_-hcn4-1 1440295 n/a 1440306 unspecified role unspecified role neutral component neutral component purified purified c-linker and cnbd domains sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) n/a 1440305 unspecified role unspecified role neutral component neutral component purified purified 8-fluoresceinyl group fluorescein fluorescein label fluorescein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction false false false 1440295 Table 1 3__5__cyclic_-hcn4-3 1440295 n/a 1440303 unspecified role unspecified role neutral component neutral component purified purified 8-fluoresceinyl group fluorescein fluorescein label fluorescein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false chemical synthesis Chemical synthesis (Chemical synthesis) n/a 1440306 unspecified role unspecified role neutral component neutral component purified purified c-linker and cnbd domains sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false 1440295 Table 1 3__5__cyclic_-hcn4-1 1440295 n/a 1440306 unspecified role unspecified role neutral component neutral component purified purified c-linker and cnbd domains sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false mbp-his tag tandem tag tandem tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) n/a 1440303 unspecified role unspecified role neutral component neutral component purified purified 8-fluoresceinyl group fluorescein fluorescein label fluorescein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction false false false 1440295 Table 1, 1c 3__5__cyclic_-hcn4-3 1440295 n/a 1440305 unspecified role unspecified role neutral component neutral component purified purified 8-fluoresceinyl group fluorescein fluorescein label fluorescein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false chemical synthesis Chemical synthesis (Chemical synthesis) n/a 1440306 unspecified role unspecified role neutral component neutral component purified purified mbp-his tag tandem tag tandem tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false c-linker and cnbd domains sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false 1440295 Table 1, 1c 3__5__cyclic_-hcn1-1 1440296 n/a 1440302 unspecified role unspecified role neutral component neutral component purified purified c-linker and cnbd domains sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 4.0 n/a 1440305 unspecified role unspecified role neutral component neutral component purified purified chemical synthesis Chemical synthesis (Chemical synthesis) Stoichiometry: 4.0 direct interaction direct interaction false false false 3h, suppl. table 4 3__5__cyclic_-hcn2-6 1440296 n/a 1440304 unspecified role unspecified role neutral component neutral component purified purified c-linker and cnbd domains sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 4.0 n/a 1440305 unspecified role unspecified role neutral component neutral component purified purified chemical synthesis Chemical synthesis (Chemical synthesis) Stoichiometry: 4.0 direct interaction direct interaction false false false 3h, suppl. table 4 hcn1-4 1440296 n/a 1440302 unspecified role unspecified role neutral component neutral component purified purified cnbd domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 4.0 direct interaction direct interaction false false false 3h, suppl. table 4 hcn1-1 1440296 n/a 1440302 unspecified role unspecified role neutral component neutral component purified purified c-linker and cnbd domains sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 direct interaction direct interaction false false false 3h, suppl. table 4 hcn2 1440296 n/a 1440304 unspecified role unspecified role neutral component neutral component purified purified c-linker and cnbd domains sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 direct interaction direct interaction false false false 3h, suppl. table 4 3__5__cyclic_-hcn4-6 1440296 n/a 1440305 unspecified role unspecified role neutral component neutral component purified purified chemical synthesis Chemical synthesis (Chemical synthesis) Stoichiometry: 4.0 n/a 1440306 unspecified role unspecified role neutral component neutral component purified purified c-linker and cnbd domains sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 4.0 direct interaction direct interaction false false false 3h, suppl. table 4 hcn4 1440296 n/a 1440306 unspecified role unspecified role neutral component neutral component purified purified c-linker and cnbd domains sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 direct interaction direct interaction false false false 3h, suppl. table 4 3__5__cyclic_-hcn1-1 1440297 n/a 1440302 unspecified role unspecified role prey prey purified purified cnbd domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) n/a 1440305 competitor competitor competitor unspecified role unspecified role chemical synthesis Chemical synthesis (Chemical synthesis) n/a 1440305 unspecified role unspecified role bait bait purified purified (6-aminohexyl)amino linker at c8 tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction false false false 1440297 Table 1 3__5__cyclic_-hcn2-1 1440297 n/a 1440304 unspecified role unspecified role prey prey purified purified c-linker and cnbd domains sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) n/a 1440305 unspecified role unspecified role bait bait purified purified (6-aminohexyl)amino linker at c8 tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false chemical synthesis Chemical synthesis (Chemical synthesis) n/a 1440305 competitor competitor competitor unspecified role unspecified role chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction false false false 1440297 Table 1 3__5__cyclic_-hcn1-4 1440297 n/a 1440302 unspecified role unspecified role prey prey purified purified cnbd domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) n/a 1440305 unspecified role unspecified role bait bait purified purified (6-aminohexyl)amino linker at c8 tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction false false false 1440297 Table 1 3__5__cyclic_am-hcn2 1440297 n/a 1440304 unspecified role unspecified role prey prey purified purified c-linker and cnbd domains sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) n/a 1440305 unspecified role unspecified role bait bait purified purified (6-aminohexyl)amino linker at c8 tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction false false false 1440297 Table 1 3__5__cyclic_-hcn4-1 1440297 n/a 1440305 unspecified role unspecified role bait bait purified purified (6-aminohexyl)amino linker at c8 tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false chemical synthesis Chemical synthesis (Chemical synthesis) n/a 1440306 unspecified role unspecified role prey prey purified purified c-linker and cnbd domains sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) n/a 1440305 competitor competitor competitor unspecified role unspecified role chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction false false false 1440297 Table 1 3__5__cyclic_am-hcn4 1440297 n/a 1440306 unspecified role unspecified role prey prey purified purified c-linker and cnbd domains sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) n/a 1440305 unspecified role unspecified role bait bait purified purified (6-aminohexyl)amino linker at c8 tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction false false false 1440297 Table 1 hcn1 1440298 n/a 1440302 unspecified role unspecified role neutral component neutral component purified purified c-linker and cnbd domains sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false O88704:p.Arg538Glu mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false c-linker domain required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain range range false ecoli Escherichia coli (strain K12) n/a 1440302 unspecified role unspecified role neutral component neutral component purified purified c-linker domain required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain range range false c-linker and cnbd domains sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false O88704:p.Arg538Glu mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false 3a, 3g, 5a, 5b, 5c cAMP Tetramerization is enhanced by treatment with cAMP, but a small amount of HCN1 tetramerizes even without cAMP. Increasing the salt concentration results in the disappearance of the tetrameric form of the protein. hcn2-5 1440298 n/a 1440304 unspecified role unspecified role neutral component neutral component purified purified c-linker domain required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain range range false c-linker and cnbd domains sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) n/a 1440304 unspecified role unspecified role neutral component neutral component purified purified c-linker domain required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain range range false c-linker and cnbd domains sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false 3b, 5b, 5c cAMP hcn4 1440298 n/a 1440306 unspecified role unspecified role neutral component neutral component purified purified c-linker domain required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain range range false c-linker and cnbd domains sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) n/a 1440306 unspecified role unspecified role neutral component neutral component purified purified c-linker and cnbd domains sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false c-linker domain required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain range range false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false 3c, 5b, 5c cAMP hcn1-1 1440299 n/a 1440302 unspecified role unspecified role neutral component neutral component purified purified c-linker and cnbd domains sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 4.0 direct interaction direct interaction false false false 3d, suppl. table 3b cAMP hcn2-5 1440299 n/a 1440304 unspecified role unspecified role neutral component neutral component purified purified c-linker and cnbd domains sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 4.0 direct interaction direct interaction false false false 3e, suppl. table 3b cAMP hcn4-1 1440299 n/a 1440306 unspecified role unspecified role neutral component neutral component purified purified c-linker and cnbd domains sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 4.0 direct interaction direct interaction false false false 3f, suppl. table 3b cAMP 3__5__cyclic_-hcn2-4 1440300 n/a 1440305 unspecified role unspecified role neutral component neutral component purified purified chemical synthesis Chemical synthesis (Chemical synthesis) n/a 1440304 unspecified role unspecified role neutral component neutral component purified purified c-linker and cnbd domains sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false 1440300 Table 1 3__5__cyclic_-hcn2-4 1440300 n/a 1440305 unspecified role unspecified role neutral component neutral component purified purified chemical synthesis Chemical synthesis (Chemical synthesis) Stoichiometry: 1.0 n/a 1440304 unspecified role unspecified role neutral component neutral component purified purified mbp-his tag tandem tag tandem tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false c-linker and cnbd domains sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 1.0 direct interaction direct interaction false false false 1440300 Table 1, S2a, S2b 3__5__cyclic_-hcn4-4 1440300 n/a 1440305 unspecified role unspecified role neutral component neutral component purified purified chemical synthesis Chemical synthesis (Chemical synthesis) Stoichiometry: 1.0 n/a 1440306 unspecified role unspecified role neutral component neutral component purified purified c-linker and cnbd domains sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false mbp-his tag tandem tag tandem tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) Stoichiometry: 1.0 direct interaction direct interaction false false false 1440300 Table 1, S2c, S2d 3__5__cyclic_am-hcn1 1440301 n/a 1440302 unspecified role unspecified role neutral component neutral component purified purified c-linker and cnbd domains sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 4.0 n/a 1440305 unspecified role unspecified role neutral component neutral component purified purified chemical synthesis Chemical synthesis (Chemical synthesis) Stoichiometry: 4.0 direct interaction direct interaction false false false 2, suppl. table 1 Rwork = 0.2 Rfree = 0.27 2.9A 3__5__cyclic_-hcn2-5 1440301 n/a 1440304 unspecified role unspecified role neutral component neutral component purified purified c-linker and cnbd domains sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 4.0 n/a 1440305 unspecified role unspecified role neutral component neutral component purified purified chemical synthesis Chemical synthesis (Chemical synthesis) Stoichiometry: 4.0 direct interaction direct interaction false false false 2d, suppl. table 1 Rwork = 0.2 Rfree = 0.27 2.9A 3__5__cyclic_-hcn4-5 1440301 n/a 1440305 unspecified role unspecified role neutral component neutral component purified purified chemical synthesis Chemical synthesis (Chemical synthesis) Stoichiometry: 4.0 n/a 1440306 unspecified role unspecified role neutral component neutral component purified purified c-linker and cnbd domains sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 4.0 direct interaction direct interaction false false false Supp. Table 1 Rwork = 0.19 Rfree = 0.28 2.5A