IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ liccardi-2011-1 EGFR nuclear translocation modulates DNA repair following cisplatin and ionizing radiation treatment. mouse-nih_3t3 Mus musculus Swiss NIH 3t3 embryo fibroblast cells nih_3t3 Mouse Swiss NIH embryo contact inhibited fibroblast cells anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Liccardi G., Hartley J.A., Hochhauser D. Affinomics - Interactions curated for the Affinomics consortium. Mutants of the NLS (nuclear localization signal) have been entered as Human in origin, as the sequence numbering -amino acids 645 to 657 (RRRHIVRKLLRR) can only refer to the numbering of the mature human protein, and Reference 22 also only refers to the Human NLS. The "wt" form of EGFR has also been entered as Human. Amino acid numbering for the NLS has been altered to aas 669-681 for the full length protein. The numbering for the L858R mutant appears to already correspond to that of the full length protein. d.hochhauser@ucl.ac.uk Cancer Res. (0008-5472) 2011 Only protein-protein interactions imex curation liccardi-2011-2 EGFR nuclear translocation modulates DNA repair following cisplatin and ionizing radiation treatment. mouse-nih_3t3 Mus musculus Swiss NIH 3t3 embryo fibroblast cells nih_3t3 Mouse Swiss NIH embryo contact inhibited fibroblast cells confocal microscopy confocal microscopy immunocytochemistry immunocytochemistry Liccardi G., Hartley J.A., Hochhauser D. Affinomics - Interactions curated for the Affinomics consortium. Mutants of the NLS (nuclear localization signal) have been entered as Human in origin, as the sequence numbering -amino acids 645 to 657 (RRRHIVRKLLRR) can only refer to the numbering of the mature human protein, and Reference 22 also only refers to the Human NLS. The "wt" form of EGFR has also been entered as Human. Amino acid numbering for the NLS has been altered to aas 669-681 for the full length protein. The numbering for the L858R mutant appears to already correspond to that of the full length protein. d.hochhauser@ucl.ac.uk Cancer Res. (0008-5472) 2011 Only protein-protein interactions imex curation liccardi-2011-3 EGFR nuclear translocation modulates DNA repair following cisplatin and ionizing radiation treatment. mouse-nih_3t3 Mus musculus Swiss NIH 3t3 embryo fibroblast cells nih_3t3 Mouse Swiss NIH embryo contact inhibited fibroblast cells pla proximity ligation assay pELISA pELISA PLA antibody detection identification by antibody antibody detection Liccardi G., Hartley J.A., Hochhauser D. Affinomics - Interactions curated for the Affinomics consortium. Mutants of the NLS (nuclear localization signal) have been entered as Human in origin, as the sequence numbering -amino acids 645 to 657 (RRRHIVRKLLRR) can only refer to the numbering of the mature human protein, and Reference 22 also only refers to the Human NLS. The "wt" form of EGFR has also been entered as Human. Amino acid numbering for the NLS has been altered to aas 669-681 for the full length protein. The numbering for the L858R mutant appears to already correspond to that of the full length protein. d.hochhauser@ucl.ac.uk Cancer Res. (0008-5472) 2011 Only protein-protein interactions imex curation prkdc_mouse DNA-dependent protein kinase catalytic subunit Prkdc Xrcc7 p460 protein protein mouse Mus musculus Mouse MAEEGTGVRCWLLQLQEFLSAADRCSAAGASYQLIRSLGQECVLSTSSAVQALQISLVFSRDFGLLVFIRKSLSIEDFRDCREEALKFLCVFLEKIDQKVMHYSLDIKNTCTSVYTKDRTAKCKIPALDLLIKLLQILRSTRLMDEFKIGELFNKFYGELASKSKLPDTVLEKVYELLGVLGEVHPSEMINHSENLFRAFLGELKTQMTSTVREPKFPVLAGCLKGLSSLLCNFTKSMEEDPQTSKEIFGFTFKAIRPQIEMKRYAVPLAGLRLLTLHASQFTACLLDNYITLFEVLSKWCSHTNVELKKAAHSALESFLRQISFTVAEDAELHKSRLKYFMEQFYGIIRNTDSNNKELAIAIRGYGLFAGPCKVINAKDVDFMYVELIQRCKQMFLTHADASEDHVYQMPSFLQSIASVLLYLDTVPEVYTPVLEHLMVVQIDSFPQYSPKMQLVCCKAIIKLFLALSEKGPVHWNCISAVVHQGLIRICSKPVVLQKDVESRSDNRSASEEVRTGRWKVPTYKDYVDLFQHLLGCDQMEDFILGDETFLFVNSSLKSLNHLLYDEFIRSVLKIVEKLDLTLEKQTVGEQEDGSTADVWVIPTSDPAANLHPAKPSDFSALINLVEFCREILPRKHVGFFEPWVYSFAYELILQSTRLPLISGFYKLLSIAVKNARKIKYFEGISPKSLKHSPEDTEKYSCFALFAKFGKEVSVKMKQYKDELLASCLTFVLSLPHDIIELDVRAYVPALQMAFKLGLSHMPLAEIGLHALKEWSVHIDKSILQPYYKDILPCLDGYLNTSTLSDETKSHWGLSALSRAAQKGFNRHVVKHLKRTRNSSPDEALSLEEIRIKVVQILGSLGGQINKSLVTATSGERMKKYVAWDAERRLSFAVPFREMKPVIYLDVFLPRVTELALSASDRQTKVAACELLHSMVMFMLGRATQMPEGQGLPPMYQLYKHTFPVLLQLACDVDQVTRQLYEPLVMQLIHWLTNNKKFESQDTVALLEAILDGIVDPVDSTLRDFCGRCVQEFLKWSIKQTTPQQQEKSPVNSKSLFKRLYSLALHPNAFKRLGAALAFNHIYKEFREEGSLVEQFVFEALVTYMESLALAHEDEKSLGTVQQCCDAIDHLRRIIEKKHVSLNKAKKRRLPQGFPPLTSLCLLDLVEWLLAHCGRPQTECRHKSMELFYKFVPLLPGNKSPSLWLKDLIKKKGISFLINTFEGGASSSDQPAGILAQPTLVYLQGPISLRGVLQWLDLLLAALECYNTFIEKETVQGQEVLGAEVQSSLLKSVAFFLESIATHSARAVEQRFGSGAPGPPSLHEEEKYNYSKCTVLVRIMEFTTTLLIASPEDCKLLEKDLCNTNLMQVLVKMICEPMSLGFNIGDVQVMNHLPSICVNLLKALRKSPYRDMLETHLKEKVTVQSVEELCSINLCSSGARQERSKLLSILSACKQLHKAGFSHVISPSQSTALNHSVGMRLLSLVYKGIVPAEERQCLQSLDPSCKSLANGLLELAFGFGGLCDHLVSLLLNSAMLSTQYLGSSQRNISFSHGEYFYSLFSEVINSELLKNLDIAVSRLMESSSDNPKMVSTVLNGMLDTSFRDRAVQKHQGLKLATAILQNWRKCDSWWAPDSAPESKTTVLSLLAKMLQIDSALSFDTNHSSFSEIFTTYASLLADTKLGLHLKGQAIILLPFFTSLREGSLENLKHILEKLIVCNFPMKSDEFPPDSLKYNNYVDCMKKFLDALELSQSPMLFQLMTDILCREQRHIMEELFQTTFKRIARQSPCVTQLNLLESVYTMFRKADLPSNVTRQAFVDRSLLTLLWHCDLDTLKEFFSRIVVDAIDVLKSRFTKLNEFTFDTQITKKMCYYKMLAVMYSRLLKDDVHSKEAKINQAFHGSRVAEGNELTKTLLKLCHDAFTENMVGESQLLEKRRLYHCAAYNCAISLISCVFNELKFYQGFLFNEKPEKNLFIFENLIDLKRCYTFPIEVEVPMERKKKYIEIRKEARDAANGASGSPHYMSSLSYLTDSSLSEEMSQFDFSTGVQSYSYSSQDRKPTTGHFQRREHQDSMTQDDIMELEMDELNQHECMAPMIALIKHMQRNVIAPKGEEGSIPKDLPPWMKFLHDKLGNASVSLNIRLFLAKLVINTEEVFRPYAKHWLSPLLQLAVCENNREGIHYMMVEIVATILSWTGLATPTGVPKDEVLANRLLRFLMKHVFHPKRAVFRHNLEIIKTLVECWKECLSIPYRLIFEKFSHKDPNSKDNSVGIQLLGIVIANNLPPYDPNCDITSAMYFEALVNNMSFVKYKEVYAAAAEVLGLILQYITERKHVIAELVCELVIKQLKQHQNTMEDKFIVCLNKIAKGFPPLADRFLNALFFLLPKFHGVMKTLCLEVVLCRAEEITGLYLQLKSKDFLQVMRHRDDERQKVCLDIVYKMVAKLKPIELRELLNPVVEFVSHPSPTCREQMYNILMWIHDNYRDQESQNDEDSQEIFKLAKDVLIQGLIDENVGLQLIIRNFWSHETRLPSNTLDRLLALNSLYSPKIEVHFLSLATNFLLEMTRMSPDYLNPIFEHPLSECEFQEYTIDPDWRFRSTVLTPMFIETQASPSILHTQTQEGPLSDQRQKPGQVRATQQQYDFTPTQASVERSSFDWLTGSSIDLLADHTVFSSETLSSSLLFSHKRTEKSQRMSCKSVGPDFGTKKLGLPDDEVDNQVKSGTPSQADILRLRRRFLKDREKLSLLYAKRGLMEQKLEKDIKSEFKMKQDAQVVLYRSYRHGDLPDIQIQHSGLITPLQAVAQKDPIIAKQLFSSLFSGILKEMNKFKTTSEKNIITQNLLQDFNRFLNTTFLFFPPFVSCIQEISCQHPDFLTLDPASVRVGCLASLQQPGGIRLLEEALLRLMPKEPPTKRVRGKTCLPPDVLRWMELAKLYRSIGEYDVLRGIFSSELGTTQDTQNALLAEARSDYCQAAKLYDEALNKLEWVDGEPTEAEKEFWELASLDCYNNLSKWKELEYCSTVNIVSENSLDLSKMWSEPFYQETYLPYVIRSKLKLLLQGEGNQSLLTFVDEAMNKELQKTVLELQYSQELSLLYILQDDIDRATYYIKNGIQIFMQNYSSIDVLLYRSRLAKLQSVQTLAEIEEFLSFICKHGDLSSLGPLRRLLKTWTSRYPDVVTDPMHIWDDIITNRCFFLSKIEERLTAPSGDHSMSVDEDEESIDREVYEPKEDVRCMLQSCRFTMKMKMIESAWKQSNFSLSMKLLKEMHKESKTREIWRVQWLHSYSQLNHCRSHTQSPREQVLNMLKTITLLDESDISNYLNKNIQASCDQSILLGTTCRIMADALSREPACLSDLEENKVNSILTLSGSNAENTETVITGLYQRAFHHLSKAVQSAEEETQLSCWGHEAAAERAHAYMTLVGFCDQQLRKVEESASQKTSAEMEAYPALVVEKMLRALKLNSSEARLKFPRLLQIIEQYSEETLNIMTKEISSIPCWQFIGWISHMMALLDKEEAIAVQHTVEEIADNYPQAIIYPFIISSESYSFKNTSSGHNNKAFVERIKSKLDHGEVIHSFINALDQLSNPDLLFKDWVSDTKDELGKNPVNKKNIEKLYERMYAALGDLRAPGLGPFRRRFIQAFGKEFVKSFGNGGSKLLTMKVDDFCKITGSLLVRMKKDSKLPGNLKEYSPWMSEFKAQFLKNELEIPGQYDGKSKPLPEYHVRISGFDERVKVMLSLRKPKRIVIRGHDEKEYPFLVKGGEDLRQDQRIEQIFEVMNAILSQDAACSQRNMQLRTYRVVPMTSRLGLIEWIENTMTLKDLLLSNMSQEEKVANNSDPKAPIRDYKDWLMKVSGKSDAGAYVLMYSRANRTETVVAFRRRESQVPPDLLKRAFVKMSTSPEAFLALRSHFASSHALLCISHWLLGIGDRHLNNFMVAMETGSVIGIDFGHAFGSATQFLPVPELMPFRLTRQFVSLMLPMKETGLMCTVMVHALRAFRSCAGLLTDTMEIFVKEPSFDWKSFEQTMLRKGGSWIQEINVTEKNWYPQHKIRYAKRKLAGANPAVITCDELYLGHEASSAFRSYTAVARGNRDYNIRAQEPESGLSEETQVKCLVDQATDPNILGRTWEGWEPWM egfr_human Epidermal growth factor receptor ERBB1 EGFR Receptor tyrosine-protein kinase erbB-1 Proto-oncogene c-ErbB-1 ERBB HER1 protein protein human Homo sapiens Human MRPSGTAGAALLALLAALCPASRALEEKKVCQGTSNKLTQLGTFEDHFLSLQRMFNNCEVVLGNLEITYVQRNYDLSFLKTIQEVAGYVLIALNTVERIPLENLQIIRGNMYYENSYALAVLSNYDANKTGLKELPMRNLQEILHGAVRFSNNPALCNVESIQWRDIVSSDFLSNMSMDFQNHLGSCQKCDPSCPNGSCWGAGEENCQKLTKIICAQQCSGRCRGKSPSDCCHNQCAAGCTGPRESDCLVCRKFRDEATCKDTCPPLMLYNPTTYQMDVNPEGKYSFGATCVKKCPRNYVVTDHGSCVRACGADSYEMEEDGVRKCKKCEGPCRKVCNGIGIGEFKDSLSINATNIKHFKNCTSISGDLHILPVAFRGDSFTHTPPLDPQELDILKTVKEITGFLLIQAWPENRTDLHAFENLEIIRGRTKQHGQFSLAVVSLNITSLGLRSLKEISDGDVIISGNKNLCYANTINWKKLFGTSGQKTKIISNRGENSCKATGQVCHALCSPEGCWGPEPRDCVSCRNVSRGRECVDKCNLLEGEPREFVENSECIQCHPECLPQAMNITCTGRGPDNCIQCAHYIDGPHCVKTCPAGVMGENNTLVWKYADAGHVCHLCHPNCTYGCTGPGLEGCPTNGPKIPSIATGMVGALLLLLVVALGIGLFMRRRHIVRKRTLRRLLQERELVEPLTPSGEAPNQALLRILKETEFKKIKVLGSGAFGTVYKGLWIPEGEKVKIPVAIKELREATSPKANKEILDEAYVMASVDNPHVCRLLGICLTSTVQLITQLMPFGCLLDYVREHKDNIGSQYLLNWCVQIAKGMNYLEDRRLVHRDLAARNVLVKTPQHVKITDFGLAKLLGAEEKEYHAEGGKVPIKWMALESILHRIYTHQSDVWSYGVTVWELMTFGSKPYDGIPASEISSILEKGERLPQPPICTIDVYMIMVKCWMIDADSRPKFRELIIEFSKMARDPQRYLVIQGDERMHLPSPTDSNFYRALMDEEDMDDVVDADEYLIPQQGFFSSPSTSRTPLLSSLSATSNNSTVACIDRNGLQSCPIKEDSFLQRYSSDPTGALTEDSIDDTFLPVPEYINQSVPKRPAGSVQNPVYHNQPLNPAPSRDPHYQDPHSTAVGNPEYLNTVQPTCVNSTFDSPAHWAQKGSHQISLDNPDYQQDFFPKEAKPNGIFKGSTAENAEYLRVAPQSSEFIGA prkdc-egfr 1540530 n/a 1540533 unspecified role unspecified role bait bait n/a 1540534 unspecified role unspecified role prey prey physical association physical association false false false 2A egfr-prkdc 1540530 n/a 1540533 unspecified role unspecified role prey prey n/a 1540534 unspecified role unspecified role bait bait P00533:p.[Arg669_Arg671delinsAlaAlaAla;Arg675Ala;Arg677Ala;Arg680_Arg681delinsAlaAla] mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false P00533:p.Leu858Arg mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false P00533:p.[Arg669_Arg671delinsAlaAlaAla;Arg675Ala;Arg677Ala;Arg680_Arg681delinsAlaAla;Leu858Arg] mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false 2B X rays, Cisplatin - a platinum containing anti-cancer drug which causes DNA cross linking. egfr-prkdc-1 1540531 n/a 1540534 unspecified role unspecified role neutral component neutral component n/a 1540533 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false 3-6 Cisplatin - a platinum containing anti-cancer drug which causes DNA cross linking. Only cells transiently transfected with wtEGFR or the EGFRvIII mutant showed clear EGFR and DNA-PKcs nuclear expression/colocalisation after Cisplatin/IR treatment. Cells transfected with other EGFR mutants showed either reduced or absent nuclear accumulation of both EGFR and DNA-PKcs. egfr-prkdc-3 1540532 n/a 1540534 unspecified role unspecified role neutral component neutral component P00533:p.[Arg669_Arg671delinsAlaAlaAla;Arg675Ala;Arg677Ala;Arg680_Arg681delinsAlaAla] mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false n/a 1540533 unspecified role unspecified role neutral component neutral component proximity proximity close-contact colocalization neighbourhood interaction false false false 7 IR (4Gy, 20 mins) and Cisplatin - a platinum containing anti-cancer drug which causes DNA cross linking.