#ID(s) interactor A	ID(s) interactor B	Alt. ID(s) interactor A	Alt. ID(s) interactor B	Alias(es) interactor A	Alias(es) interactor B	Interaction detection method(s)	Publication 1st author(s)	Publication Identifier(s)	Taxid interactor A	Taxid interactor B	Interaction type(s)	Source database(s)	Interaction identifier(s)	Confidence value(s)	Expansion method(s)	Biological role(s) interactor A	Biological role(s) interactor B	Experimental role(s) interactor A	Experimental role(s) interactor B	Type(s) interactor A	Type(s) interactor B	Xref(s) interactor A	Xref(s) interactor B	Interaction Xref(s)	Annotation(s) interactor A	Annotation(s) interactor B	Interaction annotation(s)	Host organism(s)	Interaction parameter(s)	Creation date	Update date	Checksum(s) interactor A	Checksum(s) interactor B	Interaction Checksum(s)	Negative	Feature(s) interactor A	Feature(s) interactor B	Stoichiometry(s) interactor A	Stoichiometry(s) interactor B	Identification method participant A	Identification method participant B
uniprotkb:P60664	uniprotkb:P60595	intact:EBI-9151168|uniprotkb:P10373|ensemblbacteria:AAC75086|ensemblbacteria:BAA15857	intact:EBI-1126953|uniprotkb:P10375|ensemblbacteria:AAC75084|ensemblbacteria:BAA15854	psi-mi:his6_ecoli(display_long)|uniprotkb:hisF(gene name)|psi-mi:hisF(display_short)|uniprotkb:IGP synthase cyclase subunit(gene name synonym)|uniprotkb:IGP synthase subunit HisF(gene name synonym)|uniprotkb:ImGP synthase subunit HisF(gene name synonym)|uniprotkb:JW2007(locus name)|uniprotkb:b2025(locus name)	psi-mi:his5_ecoli(display_long)|uniprotkb:IGP synthase subunit HisH(gene name synonym)|uniprotkb:hisH(gene name)|psi-mi:hisH(display_short)|uniprotkb:b2023(locus name)|uniprotkb:JW2005(locus name)|uniprotkb:ImGP synthase subunit HisH(gene name synonym)|uniprotkb:IGP synthase glutaminase subunit(gene name synonym)	psi-mi:"MI:0071"(molecular sieving)	Klem et al. (1993)	imex:IM-27949|pubmed:8494895	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0486"(UniProt)	intact:EBI-25339230|imex:IM-27949-1	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR013785(Aldolase-type TIM barrel)|refseq:NP_416529.1|interpro:IPR004651(Histidine biosynthesis, HisF)|interpro:IPR006062(Histidine biosynthesis)|interpro:IPR011060(Ribulose-phosphate binding barrel)|refseq:WP_000880182.1|ensemblbacteria:AAC75086(transcript)|ensemblbacteria:b2025(gene)|ensemblbacteria:BAA15857(gene)|ensemblbacteria:BAA15857(transcript)|go:"GO:0000105"(histidine biosynthetic process)|go:"GO:0000107"(imidazoleglycerol-phosphate synthase activity)|go:"GO:0005829"(cytosol)|go:"GO:0009382"(imidazoleglycerol-phosphate synthase complex)|go:"GO:0016829"(lyase activity)	refseq:NP_416527.1|interpro:IPR017926|interpro:IPR010139(Imidazole glycerol phosphate synthase, subunit H)|refseq:WP_001103560.1|ensemblbacteria:AAC75084(transcript)|ensemblbacteria:b2023(gene)|ensemblbacteria:BAA15854(gene)|ensemblbacteria:BAA15854(transcript)|go:"GO:0000105"(histidine biosynthetic process)|go:"GO:0000107"(imidazoleglycerol-phosphate synthase activity)|go:"GO:0004359"(glutaminase activity)|go:"GO:0006541"(glutamine metabolic process)|go:"GO:0009382"(imidazoleglycerol-phosphate synthase complex)|go:"GO:0016829"(lyase activity)|interpro:IPR029062	-	-	-	figure legend:Fig. 2|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2020/01/08	2020/01/08	rogid:CQnuQ6n9EuqzhFVLuPWJG2tr/7E83333	rogid:B+vHBU0vqoCzEXEeLQwhQLerx3883333	rigid:j64QvKaF1BqNRtQEGV3Oo/lEcEY	false	-	-	1	1	psi-mi:"MI:0818"(molecular weight estimation by coomasie staining)|psi-mi:"MI:0039"(edman degradation)	psi-mi:"MI:0818"(molecular weight estimation by coomasie staining)|psi-mi:"MI:0039"(edman degradation)
uniprotkb:P60664	uniprotkb:P60595	intact:EBI-9151168|uniprotkb:P10373|ensemblbacteria:AAC75086|ensemblbacteria:BAA15857	intact:EBI-1126953|uniprotkb:P10375|ensemblbacteria:AAC75084|ensemblbacteria:BAA15854	psi-mi:his6_ecoli(display_long)|uniprotkb:hisF(gene name)|psi-mi:hisF(display_short)|uniprotkb:IGP synthase cyclase subunit(gene name synonym)|uniprotkb:IGP synthase subunit HisF(gene name synonym)|uniprotkb:ImGP synthase subunit HisF(gene name synonym)|uniprotkb:JW2007(locus name)|uniprotkb:b2025(locus name)	psi-mi:his5_ecoli(display_long)|uniprotkb:IGP synthase subunit HisH(gene name synonym)|uniprotkb:hisH(gene name)|psi-mi:hisH(display_short)|uniprotkb:b2023(locus name)|uniprotkb:JW2005(locus name)|uniprotkb:ImGP synthase subunit HisH(gene name synonym)|uniprotkb:IGP synthase glutaminase subunit(gene name synonym)	psi-mi:"MI:0030"(cross-linking study)	Klem et al. (1993)	imex:IM-27949|pubmed:8494895	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0486"(UniProt)	intact:EBI-25339241|imex:IM-27949-3	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR013785(Aldolase-type TIM barrel)|refseq:NP_416529.1|interpro:IPR004651(Histidine biosynthesis, HisF)|interpro:IPR006062(Histidine biosynthesis)|interpro:IPR011060(Ribulose-phosphate binding barrel)|refseq:WP_000880182.1|ensemblbacteria:AAC75086(transcript)|ensemblbacteria:b2025(gene)|ensemblbacteria:BAA15857(gene)|ensemblbacteria:BAA15857(transcript)|go:"GO:0000105"(histidine biosynthetic process)|go:"GO:0000107"(imidazoleglycerol-phosphate synthase activity)|go:"GO:0005829"(cytosol)|go:"GO:0009382"(imidazoleglycerol-phosphate synthase complex)|go:"GO:0016829"(lyase activity)	refseq:NP_416527.1|interpro:IPR017926|interpro:IPR010139(Imidazole glycerol phosphate synthase, subunit H)|refseq:WP_001103560.1|ensemblbacteria:AAC75084(transcript)|ensemblbacteria:b2023(gene)|ensemblbacteria:BAA15854(gene)|ensemblbacteria:BAA15854(transcript)|go:"GO:0000105"(histidine biosynthetic process)|go:"GO:0000107"(imidazoleglycerol-phosphate synthase activity)|go:"GO:0004359"(glutaminase activity)|go:"GO:0006541"(glutamine metabolic process)|go:"GO:0009382"(imidazoleglycerol-phosphate synthase complex)|go:"GO:0016829"(lyase activity)|interpro:IPR029062	-	-	-	figure legend:Fig. 4|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2020/01/08	2020/01/08	rogid:CQnuQ6n9EuqzhFVLuPWJG2tr/7E83333	rogid:B+vHBU0vqoCzEXEeLQwhQLerx3883333	rigid:j64QvKaF1BqNRtQEGV3Oo/lEcEY	false	-	-	1	1	psi-mi:"MI:0818"(molecular weight estimation by coomasie staining)	psi-mi:"MI:0818"(molecular weight estimation by coomasie staining)
chebi:"CHEBI:27735"	rhea:"RHEA:24793"	intact:EBI-25339355|chebi:"CHEBI:7090"|chebi:"CHEBI:12100"|chebi:"CHEBI:39698"|chebi:"CHEBI:21471"	intact:EBI-25339254|intenz:4.3.2.10|intenz:3.5.1.2|rhea:"RHEA:15889"	psi-mi:"5-[(5-phospho-1-deoxy-d-ribulos-1-ylimino)methylamino]-1-(5-phospho-beta-d-ribosyl)imidazole-4-carboxamide"(display_short)|psi-mi:"CHEBI:27735"(display_long)|intact:"5-[(5-Phospho-1-deoxyribulos-1-ylamino)methylideneamino]-1-(5-phosphoribosyl)imidazole-4-carboxamide"(synonym)|intact:"N-(5'-Phospho-D-1'-ribulosylformimino)-5-amino-1-(5''-phospho-D-ribosyl)-4-imidazolecarboxamide"(synonym)|intact:"N(1)-(5'-phosphoribulosyl)-formimino-5-aminoimidazole-4-carboxamide ribonucleotide"(synonym)|intact:Phosphoribulosyl-formimino-AICAR-phosphate(synonym)|intact:PRFAR(synonym)|intact:"5-[(5-O-phosphono-1-deoxy-D-ribulos-1-ylimino)methylamino]-1-(5-O-phosphono-beta-D-ribosyl)imidazole-4-carboxamide"(iupac name)	psi-mi:igps_ecoli(display_short)|psi-mi:"RHEA:24793"(display_long)|intact:"hisF:hisH"(complex systematic name)|intact:"Glutamine amidotransferase:cyclase complex"(complex synonym)|intact:Imidazole glycerol phosphate synthase complex(complex recommended name)|intact:IGP synthase complex(complex synonym)	psi-mi:"MI:0415"(enzymatic study)	Klem et al. (1993)	imex:IM-27949|pubmed:8494895	taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)")	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	psi-mi:"MI:0414"(enzymatic reaction)	psi-mi:"MI:0486"(UniProt)	intact:EBI-25339370|imex:IM-27949-5	-	-	psi-mi:"MI:0502"(enzyme target)	psi-mi:"MI:0501"(enzyme)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0328"(small molecule)	psi-mi:"MI:1302"(stable complex)	-	go:"GO:0000107"(imidazoleglycerol-phosphate synthase activity)|evidence ontology:"ECO:0000353"|complex portal:CPX-5158(complex-primary)|intact:EBI-25339241(exp-evidence)|go:"GO:0000105"(histidine biosynthetic process)|go:"GO:0004359"(glutaminase activity)|go:"GO:0009382"(imidazoleglycerol-phosphate synthase complex)	go:"GO:0000107"(imidazoleglycerol-phosphate synthase activity)	-	curated-complex:"Essential enzyme on the pathway synthesizing L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate, catalyzing the closure of the imidazole ring thus also providing the substrate for de novo purine biosynthesis. Converts the biosynthetic intermediate 5-[(5-phospho-1-deoxy-D-ribulos-1-ylimino)methylamino]-1-(5-phospho-β-D-ribosyl)imidazole-4-carboxamide (PRFAR, CHEBI:27735) to D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate(2-) (IGP, CHEBI:58278) and 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide(2-) (AICAR, CHEBI:58475). The hisH glutaminase hydrolysis of glutamine yields ammonia which acts as a nitrogen source for the synthesis of histidine and purines."|complex-properties:"A (beta-alpha)8 barrel fold within HisF creates a putative tunnel for the transfer of ammonia from HisH to HisF, connecting the glutaminase and synthase active sites."|complex-assembly:Heterodimer	figure legend:Fig. 1, 5, 6|kinetics:Km measured in the presence of glutamine|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	km:2.4x10^-4(molar)	2020/01/08	2020/05/07	-	-	-	false	-	-	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)