#ID(s) interactor A ID(s) interactor B Alt. ID(s) interactor A Alt. ID(s) interactor B Alias(es) interactor A Alias(es) interactor B Interaction detection method(s) Publication 1st author(s) Publication Identifier(s) Taxid interactor A Taxid interactor B Interaction type(s) Source database(s) Interaction identifier(s) Confidence value(s) Expansion method(s) Biological role(s) interactor A Biological role(s) interactor B Experimental role(s) interactor A Experimental role(s) interactor B Type(s) interactor A Type(s) interactor B Xref(s) interactor A Xref(s) interactor B Interaction Xref(s) Annotation(s) interactor A Annotation(s) interactor B Interaction annotation(s) Host organism(s) Interaction parameter(s) Creation date Update date Checksum(s) interactor A Checksum(s) interactor B Interaction Checksum(s) Negative Feature(s) interactor A Feature(s) interactor B Stoichiometry(s) interactor A Stoichiometry(s) interactor B Identification method participant A Identification method participant B uniprotkb:P0C6X7-PRO_0000037315 uniprotkb:P0C6X7-PRO_0000037314 intact:EBI-25487941 intact:EBI-25487672 psi-mi:p0c6x7-pro_0000037315(display_long)|uniprotkb:ORF1ab polyprotein(gene name synonym)|uniprotkb:1a-1b(orf name)|uniprotkb:rep(gene name)|psi-mi:rep(display_short) psi-mi:p0c6x7-pro_0000037314(display_long)|uniprotkb:rep(gene name)|psi-mi:rep(display_short)|uniprotkb:ORF1ab polyprotein(gene name synonym)|uniprotkb:1a-1b(orf name) psi-mi:"MI:0069"(mass spectrometry studies of complexes) Krichel et al. (2020) doi:10.1101/2020.09.30.320762|imex:IM-28416|pubmed:33024972 taxid:694009(sars-cov)|taxid:694009(Human SARS coronavirus) taxid:694009(sars-cov)|taxid:694009(Human SARS coronavirus) psi-mi:"MI:0407"(direct interaction) psi-mi:"MI:0469"(IntAct) intact:EBI-26365523|imex:IM-28416-1 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) intact:EBI-7843867(chain-parent)|uniprotkb:P0C6X7(chain-parent) intact:EBI-7843867(chain-parent)|uniprotkb:P0C6X7(chain-parent) - chain-seq-start:3920|chain-seq-end:4117 chain-seq-start:3837|chain-seq-end:3919 figure legend:Supp. figure 2A, S4A, S5 A/B, Table S1, S5|comment:"The 2:2 tetramer is the major species detected; a 1:1 heterodimer also formed (2%) -nsp7-8 dimer affinity is low. Dimerization of the precursor molecule (Nsp 7-8-His) was detected."|comment:SARS Nsp7+8|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2020/10/05 2020/10/07 rogid:3oIsD98B0aiO0Sqlv5jCglM4pgE694009 rogid:95nEGuIgfxXAK4CVWXC+sH1eT+c694009 rigid:UBnYvNAtbcYEdfk5a9xcjeK2wxY false his tag:c-c - 2 2 psi-mi:"MI:0102"(sequence tag identification) psi-mi:"MI:0102"(sequence tag identification) uniprotkb:P0DTD1-PRO_0000449626 uniprotkb:P0DTD1-PRO_0000449625 intact:EBI-25475874 intact:EBI-25475871 psi-mi:p0dtd1-pro_0000449626(display_long)|uniprotkb:rep(gene name)|psi-mi:rep(display_short)|uniprotkb:ORF1ab polyprotein(gene name synonym)|uniprotkb:1a-1b(orf name) psi-mi:p0dtd1-pro_0000449625(display_long)|uniprotkb:rep(gene name)|psi-mi:rep(display_short)|uniprotkb:ORF1ab polyprotein(gene name synonym)|uniprotkb:1a-1b(orf name) psi-mi:"MI:0069"(mass spectrometry studies of complexes) Krichel et al. (2020) doi:10.1101/2020.09.30.320762|imex:IM-28416|pubmed:33024972 taxid:2697049(SARS-CoV-2)|taxid:2697049(SARS-CoV-2) taxid:2697049(SARS-CoV-2)|taxid:2697049(SARS-CoV-2) psi-mi:"MI:0407"(direct interaction) psi-mi:"MI:0469"(IntAct) intact:EBI-26365727|imex:IM-28416-21 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) intact:EBI-25492095(chain-parent)|uniprotkb:P0DTD1(chain-parent) intact:EBI-25492095(chain-parent)|uniprotkb:P0DTD1(chain-parent) - chain-seq-start:3943|chain-seq-end:4140 chain-seq-start:3860|chain-seq-end:3942 figure legend:Figure 1A, Supp. figure 2B, S3B, S4B, S5 C/D. Table S1, S5|comment:"The 2:2 tetramer is the major species detected; a 1:1 heterodimer also formed (4%) -nsp7-8 dimer affinity is low. Dimerization of the precursor molecule (Nsp 7-8-His) was detected."|comment:SARS2 Nsp7+8|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2020/10/05 2020/10/07 rogid:Bxev1BKtZTT1Fyf+lpsPapVosnc2697049 rogid:3AF/ThDr7adqPPYFWsiWQE2FVyY2697049 rigid:9bglx2taGFlx9F8JoF9EfOktY+Y false his tag:c-c - 2 2 psi-mi:"MI:0102"(sequence tag identification) psi-mi:"MI:0102"(sequence tag identification) uniprotkb:P0DTD1-PRO_0000449626 uniprotkb:P0DTD1-PRO_0000449626 intact:EBI-25475874 intact:EBI-25475874 psi-mi:p0dtd1-pro_0000449626(display_long)|uniprotkb:rep(gene name)|psi-mi:rep(display_short)|uniprotkb:ORF1ab polyprotein(gene name synonym)|uniprotkb:1a-1b(orf name) psi-mi:p0dtd1-pro_0000449626(display_long)|uniprotkb:rep(gene name)|psi-mi:rep(display_short)|uniprotkb:ORF1ab polyprotein(gene name synonym)|uniprotkb:1a-1b(orf name) psi-mi:"MI:0069"(mass spectrometry studies of complexes) Krichel et al. (2020) doi:10.1101/2020.09.30.320762|imex:IM-28416|pubmed:33024972 taxid:2697049(SARS-CoV-2)|taxid:2697049(SARS-CoV-2) taxid:2697049(SARS-CoV-2)|taxid:2697049(SARS-CoV-2) psi-mi:"MI:0407"(direct interaction) psi-mi:"MI:0469"(IntAct) intact:EBI-26365750|imex:IM-28416-41 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) intact:EBI-25492095(chain-parent)|uniprotkb:P0DTD1(chain-parent) intact:EBI-25492095(chain-parent)|uniprotkb:P0DTD1(chain-parent) - chain-seq-start:3943|chain-seq-end:4140 chain-seq-start:3943|chain-seq-end:4140 figure legend:Supp. figure S3B|comment:SARS2 Nsp8 homodimer|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2020/10/05 2020/10/07 rogid:Bxev1BKtZTT1Fyf+lpsPapVosnc2697049 rogid:Bxev1BKtZTT1Fyf+lpsPapVosnc2697049 rigid:s5ejA5fuX20Ni7IAubjYH38EFcI false his tag:c-c - 1 1 psi-mi:"MI:0102"(sequence tag identification) psi-mi:"MI:0102"(sequence tag identification) uniprotkb:Q98VG9-PRO_5000140215 uniprotkb:Q98VG9-PRO_5000140214 intact:EBI-26365785 intact:EBI-26365769 psi-mi:q98vg9-pro_5000140215(display_long)|uniprotkb:rep(gene name)|psi-mi:rep(display_short)|uniprotkb:ORF1ab polyprotein(gene name synonym)|uniprotkb:1a-1b(orf name) psi-mi:q98vg9-pro_5000140214(display_long)|uniprotkb:rep(gene name)|psi-mi:rep(display_short)|uniprotkb:ORF1ab polyprotein(gene name synonym)|uniprotkb:1a-1b(orf name) psi-mi:"MI:0069"(mass spectrometry studies of complexes) Krichel et al. (2020) doi:10.1101/2020.09.30.320762|imex:IM-28416|pubmed:33024972 taxid:33734(FCoV)|taxid:33734("Feline coronavirus (strain FIPV WSU-79/1146)") taxid:33734(FCoV)|taxid:33734("Feline coronavirus (strain FIPV WSU-79/1146)") psi-mi:"MI:0407"(direct interaction) psi-mi:"MI:0469"(IntAct) intact:EBI-26365761|imex:IM-28416-23 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) intact:EBI-25757479(chain-parent) intact:EBI-25757479(chain-parent) - chain-seq-start:3583|chain-seq-end:3777 chain-seq-start:3500|chain-seq-end:3582 figure legend:Figure 1B, Supp. figure 2C, S3C, S4C. Table S1, S5|comment:"The 2:1 heterotrimer is the major species detected (83%); a 1:1 heterodimer also formed (17%)."|comment:"FIPV (feline intestine peritonitis virus) Nsp7+8 heterotrimer."|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2020/10/05 2020/10/07 rogid:sbTkA3Km/7LgbeDURkgcDhLdIoQ33734 rogid:v5VgY9zTRbKwnOMtQdpluMA1PaA33734 rigid:6Ica4Ur6pxdAIdocLhTa5lpGUzo false his tag:c-c - 1 2 psi-mi:"MI:0102"(sequence tag identification) psi-mi:"MI:0102"(sequence tag identification) uniprotkb:Q98VG9-PRO_5000140215 uniprotkb:Q98VG9-PRO_5000140214 intact:EBI-26365785 intact:EBI-26365769 psi-mi:q98vg9-pro_5000140215(display_long)|uniprotkb:rep(gene name)|psi-mi:rep(display_short)|uniprotkb:ORF1ab polyprotein(gene name synonym)|uniprotkb:1a-1b(orf name) psi-mi:q98vg9-pro_5000140214(display_long)|uniprotkb:rep(gene name)|psi-mi:rep(display_short)|uniprotkb:ORF1ab polyprotein(gene name synonym)|uniprotkb:1a-1b(orf name) psi-mi:"MI:0069"(mass spectrometry studies of complexes) Krichel et al. (2020) doi:10.1101/2020.09.30.320762|imex:IM-28416|pubmed:33024972 taxid:33734(FCoV)|taxid:33734("Feline coronavirus (strain FIPV WSU-79/1146)") taxid:33734(FCoV)|taxid:33734("Feline coronavirus (strain FIPV WSU-79/1146)") psi-mi:"MI:0407"(direct interaction) psi-mi:"MI:0469"(IntAct) intact:EBI-26365806|imex:IM-28416-3 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) intact:EBI-25757479(chain-parent) intact:EBI-25757479(chain-parent) - chain-seq-start:3583|chain-seq-end:3777 chain-seq-start:3500|chain-seq-end:3582 figure legend:Figure 1B, Supp. figure 2C, Table S1, S5|comment:"FIPV (feline intestine peritonitis virus) Nsp7+8 heterodimer."|comment:"Both figure 1B and S2C have \"7+8-2\" -in green lettering, visible on the figures;it has been assumed this is a misprint as \"7+8\" is entered for all other viruses."|comment:"The 2:1 heterotrimer is the major species detected (83%); a 1:1 heterodimer also formed (17%)."|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2020/10/05 2020/10/07 rogid:sbTkA3Km/7LgbeDURkgcDhLdIoQ33734 rogid:v5VgY9zTRbKwnOMtQdpluMA1PaA33734 rigid:6Ica4Ur6pxdAIdocLhTa5lpGUzo false his tag:c-c - 1 1 psi-mi:"MI:0102"(sequence tag identification) psi-mi:"MI:0102"(sequence tag identification) uniprotkb:P0C6Y5-PRO_0000037393 uniprotkb:P0C6Y5-PRO_0000037392 intact:EBI-25684354 intact:EBI-26366034 psi-mi:p0c6y5-pro_0000037393(display_long)|uniprotkb:rep(gene name)|psi-mi:rep(display_short)|uniprotkb:ORF1ab polyprotein(gene name synonym)|uniprotkb:1a-1b(orf name) psi-mi:p0c6y5-pro_0000037392(display_long)|uniprotkb:ORF1ab polyprotein(gene name synonym)|uniprotkb:1a-1b(orf name)|uniprotkb:rep(gene name)|psi-mi:rep(display_short) psi-mi:"MI:0069"(mass spectrometry studies of complexes) Krichel et al. (2020) doi:10.1101/2020.09.30.320762|imex:IM-28416|pubmed:33024972 taxid:11151(TGEV)|taxid:11151("Porcine transmissible gastroenteritis coronavirus (strain Purdue)") taxid:11151(TGEV)|taxid:11151("Porcine transmissible gastroenteritis coronavirus (strain Purdue)") psi-mi:"MI:0407"(direct interaction) psi-mi:"MI:0469"(IntAct) intact:EBI-26366026|imex:IM-28416-5 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) intact:EBI-25684285(chain-parent) intact:EBI-25684285(chain-parent) - chain-seq-start:3558|chain-seq-end:3752 chain-seq-start:3475|chain-seq-end:3557 figure legend:Supp. figure 2D, S3D, S4D, Table S1, S5|comment:"The 2:1 heterotrimer is the major species detected (83%); a 1:1 heterodimer also formed (17%)."|comment:"TGEV (Porcine transmissible gastroenteritis coronavirus) Nsp7+8 heterotrimer."|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2020/10/05 2020/10/08 rogid:MpAryD0fTHVCWGgFLXhR/RyAoBc11151 rogid:roO+nFwv9GK9eh3iCNwY4DGGqd011151 rigid:F1Vpzs1i4JhS6gV8Tw07Cf4vdEc false his tag:c-c - 1 2 psi-mi:"MI:0102"(sequence tag identification) psi-mi:"MI:0102"(sequence tag identification) uniprotkb:P0C6Y5-PRO_0000037393 uniprotkb:P0C6Y5-PRO_0000037392 intact:EBI-25684354 intact:EBI-26366034 psi-mi:p0c6y5-pro_0000037393(display_long)|uniprotkb:rep(gene name)|psi-mi:rep(display_short)|uniprotkb:ORF1ab polyprotein(gene name synonym)|uniprotkb:1a-1b(orf name) psi-mi:p0c6y5-pro_0000037392(display_long)|uniprotkb:ORF1ab polyprotein(gene name synonym)|uniprotkb:1a-1b(orf name)|uniprotkb:rep(gene name)|psi-mi:rep(display_short) psi-mi:"MI:0069"(mass spectrometry studies of complexes) Krichel et al. (2020) doi:10.1101/2020.09.30.320762|imex:IM-28416|pubmed:33024972 taxid:11151(TGEV)|taxid:11151("Porcine transmissible gastroenteritis coronavirus (strain Purdue)") taxid:11151(TGEV)|taxid:11151("Porcine transmissible gastroenteritis coronavirus (strain Purdue)") psi-mi:"MI:0407"(direct interaction) psi-mi:"MI:0469"(IntAct) intact:EBI-26366052|imex:IM-28416-25 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) intact:EBI-25684285(chain-parent) intact:EBI-25684285(chain-parent) - chain-seq-start:3558|chain-seq-end:3752 chain-seq-start:3475|chain-seq-end:3557 figure legend:Supp. figure 2D, S3D, S4D, Table S1, S5|comment:"The 2:1 heterotrimer is the major species detected (83%); a 1:1 heterodimer also formed (17%)."|comment:"TGEV (Porcine transmissible gastroenteritis coronavirus) Nsp7+8 heterodimer."|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2020/10/05 2020/10/08 rogid:MpAryD0fTHVCWGgFLXhR/RyAoBc11151 rogid:roO+nFwv9GK9eh3iCNwY4DGGqd011151 rigid:F1Vpzs1i4JhS6gV8Tw07Cf4vdEc false his tag:c-c - 1 1 psi-mi:"MI:0102"(sequence tag identification) psi-mi:"MI:0102"(sequence tag identification) uniprotkb:K9N638-PRO_0000422461 uniprotkb:K9N638-PRO_0000422460 intact:EBI-26366276 intact:EBI-26366263 psi-mi:k9n638-pro_0000422461(display_long)|uniprotkb:ORF1a polyprotein(gene name synonym)|uniprotkb:1a(orf name) psi-mi:k9n638-pro_0000422460(display_long)|uniprotkb:ORF1a polyprotein(gene name synonym)|uniprotkb:1a(orf name) psi-mi:"MI:0069"(mass spectrometry studies of complexes) Krichel et al. (2020) doi:10.1101/2020.09.30.320762|imex:IM-28416|pubmed:33024972 taxid:1263720(cvemc)|taxid:1263720("Middle East respiratory syndrome-related coronavirus (isolate United Kingdom/H123990006/2012) (Betacoronavirus England 1)") taxid:1263720(cvemc)|taxid:1263720("Middle East respiratory syndrome-related coronavirus (isolate United Kingdom/H123990006/2012) (Betacoronavirus England 1)") psi-mi:"MI:0407"(direct interaction) psi-mi:"MI:0469"(IntAct) intact:EBI-26366109|imex:IM-28416-27 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) intact:EBI-25618448(chain-parent) intact:EBI-25618448(chain-parent) - chain-seq-start:3929|chain-seq-end:4127 chain-seq-start:3846|chain-seq-end:3928 figure legend:Figure 1D, Supp. figure 2G, S3G, S4G. Table S1, S5|comment:"MERS-CoV nsp7+8 forms heterodimers (1:1), -trimers (2:1) and tetramers (2:2) (73 %, 8 % and 19 %, respectively). However, heterotrimer (2:1) formation by CID-MS/MS cannot be confirmed. The dominant intermediate is stated to be nsp10-11-His 6."|comment:MERS Nsp7+8 heterotetramer|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2020/10/05 2020/10/08 rogid:aNT0rd2MwJasTBwTfZfd4JvJXqA1263720 rogid:IrKwW/DkKKm8c15jc8rQZbCGROc1263720 rigid:Cs2Z4SDMhHuC1T59pz1zXM5Npp4 false - - 2 2 psi-mi:"MI:0102"(sequence tag identification) psi-mi:"MI:0102"(sequence tag identification) uniprotkb:K9N638-PRO_0000422461 uniprotkb:K9N638-PRO_0000422460 intact:EBI-26366276 intact:EBI-26366263 psi-mi:k9n638-pro_0000422461(display_long)|uniprotkb:ORF1a polyprotein(gene name synonym)|uniprotkb:1a(orf name) psi-mi:k9n638-pro_0000422460(display_long)|uniprotkb:ORF1a polyprotein(gene name synonym)|uniprotkb:1a(orf name) psi-mi:"MI:0069"(mass spectrometry studies of complexes) Krichel et al. (2020) doi:10.1101/2020.09.30.320762|imex:IM-28416|pubmed:33024972 taxid:1263720(cvemc)|taxid:1263720("Middle East respiratory syndrome-related coronavirus (isolate United Kingdom/H123990006/2012) (Betacoronavirus England 1)") taxid:1263720(cvemc)|taxid:1263720("Middle East respiratory syndrome-related coronavirus (isolate United Kingdom/H123990006/2012) (Betacoronavirus England 1)") psi-mi:"MI:0407"(direct interaction) psi-mi:"MI:0469"(IntAct) intact:EBI-26366460|imex:IM-28416-9 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) intact:EBI-25618448(chain-parent) intact:EBI-25618448(chain-parent) - chain-seq-start:3929|chain-seq-end:4127 chain-seq-start:3846|chain-seq-end:3928 figure legend:Figure 1D, Supp. figure 2G, S3G, S4G. Table S1, S5|comment:"MERS-CoV nsp7+8 forms heterodimers (1:1), -trimers (2:1) and tetramers (2:2) (73 %, 8 % and 19 %, respectively). However, heterotrimer (2:1) formation by CID-MS/MS cannot be confirmed. The dominant intermediate is stated to be nsp10-11-His 6."|comment:MERS Nsp7+8 heterodimer|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2020/10/05 2020/10/08 rogid:aNT0rd2MwJasTBwTfZfd4JvJXqA1263720 rogid:IrKwW/DkKKm8c15jc8rQZbCGROc1263720 rigid:Cs2Z4SDMhHuC1T59pz1zXM5Npp4 false - - 1 1 psi-mi:"MI:0102"(sequence tag identification) psi-mi:"MI:0102"(sequence tag identification) uniprotkb:P0C6X1-PRO_0000037300 uniprotkb:P0C6X1-PRO_0000037299 intact:EBI-26366585 intact:EBI-26366570 psi-mi:p0c6x1-pro_0000037300(display_long)|uniprotkb:rep(gene name)|psi-mi:rep(display_short)|uniprotkb:ORF1ab polyprotein(gene name synonym)|uniprotkb:1a-1b(orf name) psi-mi:p0c6x1-pro_0000037299(display_long)|uniprotkb:rep(gene name)|psi-mi:rep(display_short)|uniprotkb:1a-1b(orf name)|uniprotkb:ORF1ab polyprotein(gene name synonym) psi-mi:"MI:0069"(mass spectrometry studies of complexes) Krichel et al. (2020) doi:10.1101/2020.09.30.320762|imex:IM-28416|pubmed:33024972 taxid:11137(hcov-229e)|taxid:11137(Human coronavirus 229E) taxid:11137(hcov-229e)|taxid:11137(Human coronavirus 229E) psi-mi:"MI:0407"(direct interaction) psi-mi:"MI:0469"(IntAct) intact:EBI-26366564|imex:IM-28416-29 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) intact:EBI-25637276(chain-parent) intact:EBI-25637276(chain-parent) - chain-seq-start:3630|chain-seq-end:3824 chain-seq-start:3547|chain-seq-end:3629 figure legend:Figure 1C, Supp. figure 2E, S3E, S4E. Table S1,S5.|comment:"HCoV-229E nsp7+8 forms three major types of oligomers with slightly different efficiencies: heterodimers (1:1), heterotrimers (2:1) and heterotetramers (2:2): 20 %, 12 % and 69 %; respectively. Dimerization of the precursor molecule (Nsp 7-8-His) was detected."|comment:229E Nsp7+8 heterotetramer|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2020/10/05 2020/10/08 rogid:gxlb75DWNpongHRQRNBPiXJXcsY11137 rogid:sz9PBIP0Z69WOYwozS+EX/AiC3s11137 rigid:8R29z20QhXlF7rF4XlK13rk6+kk false his tag:c-c - 2 2 psi-mi:"MI:0102"(sequence tag identification) psi-mi:"MI:0102"(sequence tag identification) uniprotkb:P0C6X1-PRO_0000037300 uniprotkb:P0C6X1-PRO_0000037299 intact:EBI-26366585 intact:EBI-26366570 psi-mi:p0c6x1-pro_0000037300(display_long)|uniprotkb:rep(gene name)|psi-mi:rep(display_short)|uniprotkb:ORF1ab polyprotein(gene name synonym)|uniprotkb:1a-1b(orf name) psi-mi:p0c6x1-pro_0000037299(display_long)|uniprotkb:rep(gene name)|psi-mi:rep(display_short)|uniprotkb:1a-1b(orf name)|uniprotkb:ORF1ab polyprotein(gene name synonym) psi-mi:"MI:0069"(mass spectrometry studies of complexes) Krichel et al. (2020) doi:10.1101/2020.09.30.320762|imex:IM-28416|pubmed:33024972 taxid:11137(hcov-229e)|taxid:11137(Human coronavirus 229E) taxid:11137(hcov-229e)|taxid:11137(Human coronavirus 229E) psi-mi:"MI:0407"(direct interaction) psi-mi:"MI:0469"(IntAct) intact:EBI-26366593|imex:IM-28416-43 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) intact:EBI-25637276(chain-parent) intact:EBI-25637276(chain-parent) - chain-seq-start:3630|chain-seq-end:3824 chain-seq-start:3547|chain-seq-end:3629 figure legend:Figure 1C, Supp. figure 2E, S3E, S4E. Table S1,S5.|comment:"HCoV-229E nsp7+8 forms three major types of oligomers with slightly different efficiencies: heterodimers (1:1), heterotrimers (2:1) and heterotetramers (2:2): 20 %, 12 % and 69 %; respectively. Dimerization of the precursor molecule (Nsp 7-8-His) was detected."|comment:229E Nsp7+8 heterodimer.|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2020/10/05 2020/10/08 rogid:gxlb75DWNpongHRQRNBPiXJXcsY11137 rogid:sz9PBIP0Z69WOYwozS+EX/AiC3s11137 rigid:8R29z20QhXlF7rF4XlK13rk6+kk false his tag:c-c - 1 1 psi-mi:"MI:0102"(sequence tag identification) psi-mi:"MI:0102"(sequence tag identification) uniprotkb:P0C6X1-PRO_0000037300 uniprotkb:P0C6X1-PRO_0000037299 intact:EBI-26366585 intact:EBI-26366570 psi-mi:p0c6x1-pro_0000037300(display_long)|uniprotkb:rep(gene name)|psi-mi:rep(display_short)|uniprotkb:ORF1ab polyprotein(gene name synonym)|uniprotkb:1a-1b(orf name) psi-mi:p0c6x1-pro_0000037299(display_long)|uniprotkb:rep(gene name)|psi-mi:rep(display_short)|uniprotkb:1a-1b(orf name)|uniprotkb:ORF1ab polyprotein(gene name synonym) psi-mi:"MI:0069"(mass spectrometry studies of complexes) Krichel et al. (2020) doi:10.1101/2020.09.30.320762|imex:IM-28416|pubmed:33024972 taxid:11137(hcov-229e)|taxid:11137(Human coronavirus 229E) taxid:11137(hcov-229e)|taxid:11137(Human coronavirus 229E) psi-mi:"MI:0407"(direct interaction) psi-mi:"MI:0469"(IntAct) intact:EBI-26366601|imex:IM-28416-31 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) intact:EBI-25637276(chain-parent) intact:EBI-25637276(chain-parent) - chain-seq-start:3630|chain-seq-end:3824 chain-seq-start:3547|chain-seq-end:3629 figure legend:Figure 1C, Supp. figure 2E, S3E, S4E. Table S1,S5.|comment:"HCoV-229E nsp7+8 forms three major types of oligomers with slightly different efficiencies: heterodimers (1:1), heterotrimers (2:1) and heterotetramers (2:2): 20 %, 12 % and 69 %; respectively. Dimerization of the precursor molecule (Nsp 7-8-His) was detected."|comment:229E Nsp7+8 heterotrimer.|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2020/10/05 2020/10/08 rogid:gxlb75DWNpongHRQRNBPiXJXcsY11137 rogid:sz9PBIP0Z69WOYwozS+EX/AiC3s11137 rigid:8R29z20QhXlF7rF4XlK13rk6+kk false his tag:c-c - 1 2 psi-mi:"MI:0102"(sequence tag identification) psi-mi:"MI:0102"(sequence tag identification) uniprotkb:P0C6Y4-PRO_0000037376 uniprotkb:P0C6Y4-PRO_0000037375 intact:EBI-26366633 intact:EBI-26366617 psi-mi:p0c6y4-pro_0000037376(display_long)|uniprotkb:rep(gene name)|psi-mi:rep(display_short)|uniprotkb:ORF1ab polyprotein(gene name synonym)|uniprotkb:1a-1b(orf name) psi-mi:p0c6y4-pro_0000037375(display_long)|uniprotkb:rep(gene name)|psi-mi:rep(display_short)|uniprotkb:ORF1ab polyprotein(gene name synonym)|uniprotkb:1a-1b(orf name) psi-mi:"MI:0069"(mass spectrometry studies of complexes) Krichel et al. (2020) doi:10.1101/2020.09.30.320762|imex:IM-28416|pubmed:33024972 taxid:229032(pedv)|taxid:229032("Porcine epidemic diarrhea virus (strain CV777)") taxid:229032(pedv)|taxid:229032("Porcine epidemic diarrhea virus (strain CV777)") psi-mi:"MI:0407"(direct interaction) psi-mi:"MI:0469"(IntAct) intact:EBI-26366609|imex:IM-28416-45 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) intact:EBI-25590344(chain-parent) intact:EBI-25590344(chain-parent) - chain-seq-start:3663|chain-seq-end:3857 chain-seq-start:3580|chain-seq-end:3662 figure legend:Supp. figure 2F, S3F, S4F. Table S1, S5|comment:"PEDV nsp7+8 forms three major types of oligomers with slightly different efficiencies: heterodimers (1:1), heterotrimers (2:1) and heterotetramers (2:2): 52 %, 6 % and 42 %, respectively. Dimerization of the precursor molecule (Nsp 7-8-His) was detected."|comment:PEDV Nsp7+8 heterotetramer|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2020/10/05 2020/10/08 rogid:lNewVfLi7ukX8y+uaQNSOT6bUVM229032 rogid:lpQG23x4VT7TydE+U6ADt/+eq0k229032 rigid:BwdJpha62Ej/Jm+13TYpUpuRACc false his tag:c-c - 2 2 psi-mi:"MI:0102"(sequence tag identification) psi-mi:"MI:0102"(sequence tag identification) uniprotkb:P0C6Y4-PRO_0000037376 uniprotkb:P0C6Y4-PRO_0000037375 intact:EBI-26366633 intact:EBI-26366617 psi-mi:p0c6y4-pro_0000037376(display_long)|uniprotkb:rep(gene name)|psi-mi:rep(display_short)|uniprotkb:ORF1ab polyprotein(gene name synonym)|uniprotkb:1a-1b(orf name) psi-mi:p0c6y4-pro_0000037375(display_long)|uniprotkb:rep(gene name)|psi-mi:rep(display_short)|uniprotkb:ORF1ab polyprotein(gene name synonym)|uniprotkb:1a-1b(orf name) psi-mi:"MI:0069"(mass spectrometry studies of complexes) Krichel et al. (2020) doi:10.1101/2020.09.30.320762|imex:IM-28416|pubmed:33024972 taxid:229032(pedv)|taxid:229032("Porcine epidemic diarrhea virus (strain CV777)") taxid:229032(pedv)|taxid:229032("Porcine epidemic diarrhea virus (strain CV777)") psi-mi:"MI:0407"(direct interaction) psi-mi:"MI:0469"(IntAct) intact:EBI-26366639|imex:IM-28416-33 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) intact:EBI-25590344(chain-parent) intact:EBI-25590344(chain-parent) - chain-seq-start:3663|chain-seq-end:3857 chain-seq-start:3580|chain-seq-end:3662 figure legend:Supp. figure 2F, S3F, S4F. Table S1, S5|comment:"PEDV nsp7+8 forms three major types of oligomers with slightly different efficiencies: heterodimers (1:1), heterotrimers (2:1) and heterotetramers (2:2): 52 %, 6 % and 42 %, respectively. Dimerization of the precursor molecule (Nsp 7-8-His) was detected."|comment:PEDV Nsp7+8 heterodimer|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2020/10/05 2020/10/08 rogid:lNewVfLi7ukX8y+uaQNSOT6bUVM229032 rogid:lpQG23x4VT7TydE+U6ADt/+eq0k229032 rigid:BwdJpha62Ej/Jm+13TYpUpuRACc false his tag:c-c - 1 1 psi-mi:"MI:0102"(sequence tag identification) psi-mi:"MI:0102"(sequence tag identification) uniprotkb:P0C6X7-PRO_0000037315 uniprotkb:P0C6X7-PRO_0000037315 intact:EBI-25487941 intact:EBI-25487941 psi-mi:p0c6x7-pro_0000037315(display_long)|uniprotkb:ORF1ab polyprotein(gene name synonym)|uniprotkb:1a-1b(orf name)|uniprotkb:rep(gene name)|psi-mi:rep(display_short) psi-mi:p0c6x7-pro_0000037315(display_long)|uniprotkb:ORF1ab polyprotein(gene name synonym)|uniprotkb:1a-1b(orf name)|uniprotkb:rep(gene name)|psi-mi:rep(display_short) psi-mi:"MI:0069"(mass spectrometry studies of complexes) Krichel et al. (2020) doi:10.1101/2020.09.30.320762|imex:IM-28416|pubmed:33024972 taxid:694009(sars-cov)|taxid:694009(Human SARS coronavirus) taxid:694009(sars-cov)|taxid:694009(Human SARS coronavirus) psi-mi:"MI:0407"(direct interaction) psi-mi:"MI:0469"(IntAct) intact:EBI-26367999|imex:IM-28416-39 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) intact:EBI-7843867(chain-parent)|uniprotkb:P0C6X7(chain-parent) intact:EBI-7843867(chain-parent)|uniprotkb:P0C6X7(chain-parent) - chain-seq-start:3920|chain-seq-end:4117 chain-seq-start:3920|chain-seq-end:4117 figure legend:Supp. figure S3A|comment:SARS Nsp8 homodimer|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2020/10/05 2020/10/13 rogid:3oIsD98B0aiO0Sqlv5jCglM4pgE694009 rogid:3oIsD98B0aiO0Sqlv5jCglM4pgE694009 rigid:LyGWLWmCmpRzKVeBv9D43fON4Aw false his tag:c-c - 1 1 psi-mi:"MI:0102"(sequence tag identification) psi-mi:"MI:0102"(sequence tag identification) uniprotkb:P0C6Y4-PRO_0000037376 uniprotkb:P0C6Y4-PRO_0000037375 intact:EBI-26366633 intact:EBI-26366617 psi-mi:p0c6y4-pro_0000037376(display_long)|uniprotkb:rep(gene name)|psi-mi:rep(display_short)|uniprotkb:ORF1ab polyprotein(gene name synonym)|uniprotkb:1a-1b(orf name) psi-mi:p0c6y4-pro_0000037375(display_long)|uniprotkb:rep(gene name)|psi-mi:rep(display_short)|uniprotkb:ORF1ab polyprotein(gene name synonym)|uniprotkb:1a-1b(orf name) psi-mi:"MI:0069"(mass spectrometry studies of complexes) Krichel et al. (2020) doi:10.1101/2020.09.30.320762|imex:IM-28416|pubmed:33024972 taxid:229032(pedv)|taxid:229032("Porcine epidemic diarrhea virus (strain CV777)") taxid:229032(pedv)|taxid:229032("Porcine epidemic diarrhea virus (strain CV777)") psi-mi:"MI:0407"(direct interaction) psi-mi:"MI:0469"(IntAct) intact:EBI-26368006|imex:IM-28416-19 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) intact:EBI-25590344(chain-parent) intact:EBI-25590344(chain-parent) - chain-seq-start:3663|chain-seq-end:3857 chain-seq-start:3580|chain-seq-end:3662 figure legend:Supp. figure 2F, S3F, S4F. Table S1, S5|comment:"PEDV nsp7+8 forms three major types of oligomers with slightly different efficiencies: heterodimers (1:1), heterotrimers (2:1) and heterotetramers (2:2): 52 %, 6 % and 42 %, respectively. Dimerization of the precursor molecule (Nsp 7-8-His) was detected."|comment:PEDV Nsp7+8 heterotrimer|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2020/10/05 2020/10/13 rogid:lNewVfLi7ukX8y+uaQNSOT6bUVM229032 rogid:lpQG23x4VT7TydE+U6ADt/+eq0k229032 rigid:BwdJpha62Ej/Jm+13TYpUpuRACc false his tag:c-c - 1 2 psi-mi:"MI:0102"(sequence tag identification) psi-mi:"MI:0102"(sequence tag identification) uniprotkb:P0DTD1-PRO_0000449626 uniprotkb:P0DTD1-PRO_0000449625 intact:EBI-25475874 intact:EBI-25475871 psi-mi:p0dtd1-pro_0000449626(display_long)|uniprotkb:rep(gene name)|psi-mi:rep(display_short)|uniprotkb:ORF1ab polyprotein(gene name synonym)|uniprotkb:1a-1b(orf name) psi-mi:p0dtd1-pro_0000449625(display_long)|uniprotkb:rep(gene name)|psi-mi:rep(display_short)|uniprotkb:ORF1ab polyprotein(gene name synonym)|uniprotkb:1a-1b(orf name) psi-mi:"MI:0038"(dynamic light scattering) Krichel et al. (2020) doi:10.1101/2020.09.30.320762|imex:IM-28416|pubmed:33024972 taxid:2697049(SARS-CoV-2)|taxid:2697049(SARS-CoV-2) taxid:2697049(SARS-CoV-2)|taxid:2697049(SARS-CoV-2) psi-mi:"MI:0407"(direct interaction) psi-mi:"MI:0469"(IntAct) intact:EBI-26366671|imex:IM-28416-11 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) intact:EBI-25492095(chain-parent)|uniprotkb:P0DTD1(chain-parent) intact:EBI-25492095(chain-parent)|uniprotkb:P0DTD1(chain-parent) - chain-seq-start:3943|chain-seq-end:4140 chain-seq-start:3860|chain-seq-end:3942 figure legend:"Figure 3 A/B (Figure 4 A/B in the text)"|comment:|comment:SARS2 Nsp7+8|comment:"A heterotetramer is likely the prevailing species in solution (Figure 4 B)."|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2020/10/05 2020/10/08 rogid:Bxev1BKtZTT1Fyf+lpsPapVosnc2697049 rogid:3AF/ThDr7adqPPYFWsiWQE2FVyY2697049 rigid:9bglx2taGFlx9F8JoF9EfOktY+Y false his tag:c-c - 2 2 psi-mi:"MI:0396"(predetermined participant) psi-mi:"MI:0396"(predetermined participant) uniprotkb:P0DTD1-PRO_0000449626 uniprotkb:P0DTD1-PRO_0000449625 intact:EBI-25475874 intact:EBI-25475871 psi-mi:p0dtd1-pro_0000449626(display_long)|uniprotkb:rep(gene name)|psi-mi:rep(display_short)|uniprotkb:ORF1ab polyprotein(gene name synonym)|uniprotkb:1a-1b(orf name) psi-mi:p0dtd1-pro_0000449625(display_long)|uniprotkb:rep(gene name)|psi-mi:rep(display_short)|uniprotkb:ORF1ab polyprotein(gene name synonym)|uniprotkb:1a-1b(orf name) psi-mi:"MI:0826"(x ray scattering) Krichel et al. (2020) doi:10.1101/2020.09.30.320762|imex:IM-28416|pubmed:33024972 taxid:2697049(SARS-CoV-2)|taxid:2697049(SARS-CoV-2) taxid:2697049(SARS-CoV-2)|taxid:2697049(SARS-CoV-2) psi-mi:"MI:0407"(direct interaction) psi-mi:"MI:0469"(IntAct) intact:EBI-26366693|imex:IM-28416-13 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) intact:EBI-25492095(chain-parent)|uniprotkb:P0DTD1(chain-parent) intact:EBI-25492095(chain-parent)|uniprotkb:P0DTD1(chain-parent) - chain-seq-start:3943|chain-seq-end:4140 chain-seq-start:3860|chain-seq-end:3942 figure legend:"Figure 3 C-F (Figure 4 B/E/F in the text), Supp. figure 14. Table S3 and S4."|comment:|comment:SARS2 Nsp7+8|comment:"The nsp7+8 (2:2) heterotetramer is the prevailing stoichiometry in solution at physiological concentrations;larger assemblies are observed at very high solute concentrations."|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2020/10/05 2020/10/08 rogid:Bxev1BKtZTT1Fyf+lpsPapVosnc2697049 rogid:3AF/ThDr7adqPPYFWsiWQE2FVyY2697049 rigid:9bglx2taGFlx9F8JoF9EfOktY+Y false his tag:c-c - 2 2 psi-mi:"MI:0396"(predetermined participant) psi-mi:"MI:0396"(predetermined participant) uniprotkb:P0C6X1-PRO_0000037297 uniprotkb:P0C6X1 intact:EBI-26366728 intact:EBI-25637276|uniprotkb:Q05002|uniprotkb:Q9DLN0|uniprotkb:Q9DLN1 psi-mi:p0c6x1-pro_0000037297(display_long)|uniprotkb:rep(gene name)|psi-mi:rep(display_short)|uniprotkb:ORF1ab polyprotein(gene name synonym)|uniprotkb:1a-1b(orf name) psi-mi:r1ab_cvh22(display_long)|uniprotkb:rep(gene name)|psi-mi:rep(display_short)|uniprotkb:ORF1ab polyprotein(gene name synonym)|uniprotkb:1a-1b(orf name) psi-mi:"MI:0435"(protease assay) Krichel et al. (2020) doi:10.1101/2020.09.30.320762|imex:IM-28416|pubmed:33024972 taxid:11137(hcov-229e)|taxid:11137(Human coronavirus 229E) taxid:11137(hcov-229e)|taxid:11137(Human coronavirus 229E) psi-mi:"MI:0570"(protein cleavage) psi-mi:"MI:0469"(IntAct) intact:EBI-26366721|imex:IM-28416-47 - - psi-mi:"MI:0501"(enzyme) psi-mi:"MI:0502"(enzyme target) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) intact:EBI-25637276(chain-parent) go:"GO:0003678"(DNA helicase activity)|go:"GO:0003723"(RNA binding)|go:"GO:0039648"(modulation by virus of host protein ubiquitination)|go:"GO:0039694"(viral RNA genome replication)|go:"GO:0042802"(identical protein binding)|go:"GO:0044172"(host cell endoplasmic reticulum-Golgi intermediate compartment)|go:"GO:0044220"(host cell perinuclear region of cytoplasm)|go:"GO:0140603"(obsolete ATP hydrolysis activity)|go:"GO:0003724"(RNA helicase activity)|go:"GO:0003968"(RNA-directed 5'-3' RNA polymerase activity)|go:"GO:0004197"(cysteine-type endopeptidase activity)|go:"GO:0004519"(endonuclease activity)|go:"GO:0004532"(exoribonuclease activity)|go:"GO:0004843"(thiol-dependent deubiquitinase)|go:"GO:0005524"(ATP binding)|go:"GO:0006351"(transcription, DNA-templated)|go:"GO:0008168"(methyltransferase activity)|go:"GO:0008270"(zinc ion binding)|go:"GO:0016021"(integral component of membrane)|go:"GO:0019082"(viral protein processing)|go:"GO:0032259"(methylation)|go:"GO:0033644"(host cell membrane)|go:"GO:0039520"(induction by virus of host autophagy)|go:"GO:0039548"(suppression by virus of host viral-induced cytoplasmic pattern recognition receptor signaling pathway via inhibition of IRF3 activity)|interpro:IPR044309|interpro:IPR044313|interpro:IPR044330|interpro:IPR044343|interpro:IPR044353|interpro:IPR044356|interpro:IPR044369|interpro:IPR044371|interpro:IPR044385|interpro:IPR044401|interpro:IPR043609|interpro:IPR043608|interpro:IPR043606|interpro:IPR043613|interpro:IPR043615|interpro:IPR043611|interpro:IPR043612|interpro:IPR043610|interpro:IPR001205(RNA-directed RNA polymerase, P3D protein)|interpro:IPR043174|refseq:NP_073549.1|rcsb pdb:1P9S|rcsb pdb:2J97|rcsb pdb:2J98|rcsb pdb:3EJG|rcsb pdb:4RS4|rcsb pdb:4S1T|interpro:IPR027351|interpro:IPR032505|interpro:IPR009461(Coronavirus NSP13)|interpro:IPR027352|interpro:IPR041679|interpro:IPR037227|interpro:IPR002589(Appr-1-p processing)|interpro:IPR036333|interpro:IPR009466(NSP11)|interpro:IPR042515|interpro:IPR038123|interpro:IPR014828(Nsp7 replicase)|interpro:IPR037204|interpro:IPR014829(Nsp8 replicase)|interpro:IPR037230|interpro:IPR014822(Nsp9 replicase)|interpro:IPR036499|interpro:IPR027417|interpro:IPR008740(Peptidase C30, Coronavirus endopeptidase)|interpro:IPR013016(Peptidase C30/C16)|interpro:IPR009003(Peptidase, trypsin-like serine and cysteine)|interpro:IPR007094(RNA-directed RNA polymerase, positive-strand RNA eukaryotic virus)|interpro:IPR009469(RNA polymerase, N-terminal, coronaviral)|interpro:IPR018995|interpro:IPR029063|interpro:IPR043472|interpro:IPR043477|interpro:IPR043502|interpro:IPR043504|interpro:IPR044357|interpro:IPR043178 go:"GO:0004197"(cysteine-type endopeptidase activity) chain-seq-start:2966|chain-seq-end:3267 - figure legend:Supp. figure 1A, number 1|comment:HCoV-229E Nsp 7+8 and HCoV-229E Nsp5.|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2020/10/05 2020/10/08 rogid:P7IJ5gWxHpiPJos9YTv1FcuBUH011137 rogid:0gZQs8uWrbhUOqxjkf0D+VKHsoA11137 rigid:AQASI265tc7HfTiOh5U6RNLVEWk false maltose binding protein tag:n-n|his tag:c-c his tag:c-c|sufficient binding region:3547-3824 - - psi-mi:"MI:0816"(molecular weight estimation by staining) psi-mi:"MI:0816"(molecular weight estimation by staining) uniprotkb:P0C6X1-PRO_0000037297 uniprotkb:P0C6Y4 intact:EBI-26366728 intact:EBI-25590344|uniprotkb:Q91AV2 psi-mi:p0c6x1-pro_0000037297(display_long)|uniprotkb:rep(gene name)|psi-mi:rep(display_short)|uniprotkb:ORF1ab polyprotein(gene name synonym)|uniprotkb:1a-1b(orf name) psi-mi:r1ab_pedv7(display_long)|uniprotkb:rep(gene name)|psi-mi:rep(display_short)|uniprotkb:ORF1ab polyprotein(gene name synonym)|uniprotkb:1a-1b(orf name) psi-mi:"MI:0435"(protease assay) Krichel et al. (2020) doi:10.1101/2020.09.30.320762|imex:IM-28416|pubmed:33024972 taxid:11137(hcov-229e)|taxid:11137(Human coronavirus 229E) taxid:229032(pedv)|taxid:229032("Porcine epidemic diarrhea virus (strain CV777)") psi-mi:"MI:0570"(protein cleavage) psi-mi:"MI:0469"(IntAct) intact:EBI-26366740|imex:IM-28416-49 - - psi-mi:"MI:0501"(enzyme) psi-mi:"MI:0502"(enzyme target) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) intact:EBI-25637276(chain-parent) go:"GO:0003678"(DNA helicase activity)|go:"GO:0003723"(RNA binding)|go:"GO:0003968"(RNA-directed 5'-3' RNA polymerase activity)|go:"GO:0004197"(cysteine-type endopeptidase activity)|go:"GO:0004519"(endonuclease activity)|go:"GO:0004532"(exoribonuclease activity)|go:"GO:0004843"(thiol-dependent deubiquitinase)|go:"GO:0005524"(ATP binding)|go:"GO:0006351"(transcription, DNA-templated)|go:"GO:0008168"(methyltransferase activity)|go:"GO:0008270"(zinc ion binding)|go:"GO:0016021"(integral component of membrane)|go:"GO:0019079"(viral genome replication)|go:"GO:0019082"(viral protein processing)|go:"GO:0030430"(host cell cytoplasm)|go:"GO:0032259"(methylation)|go:"GO:0033644"(host cell membrane)|go:"GO:0039520"(induction by virus of host autophagy)|go:"GO:0039548"(suppression by virus of host viral-induced cytoplasmic pattern recognition receptor signaling pathway via inhibition of IRF3 activity)|go:"GO:0039644"(suppression by virus of host NF-kappaB cascade)|go:"GO:0003724"(RNA helicase activity)|go:"GO:0042025"(host cell nucleus)|go:"GO:0042802"(identical protein binding)|go:"GO:0044172"(host cell endoplasmic reticulum-Golgi intermediate compartment)|go:"GO:0044220"(host cell perinuclear region of cytoplasm)|go:"GO:0140603"(obsolete ATP hydrolysis activity)|go:"GO:0039648"(modulation by virus of host protein ubiquitination)|interpro:IPR043178|interpro:IPR044357|interpro:IPR027351|interpro:IPR032505|interpro:IPR009461(Coronavirus NSP13)|refseq:NP_598309.2|rcsb pdb:5HIY|rcsb pdb:5HIZ|interpro:IPR043613|interpro:IPR043615|interpro:IPR043611|interpro:IPR043612|interpro:IPR043610|interpro:IPR001205(RNA-directed RNA polymerase, P3D protein)|interpro:IPR043609|interpro:IPR043608|interpro:IPR043606|interpro:IPR041679|interpro:IPR037227|interpro:IPR002589(Appr-1-p processing)|interpro:IPR036333|interpro:IPR009466(NSP11)|interpro:IPR042515|interpro:IPR038123|interpro:IPR014828(Nsp7 replicase)|interpro:IPR037204|interpro:IPR014829(Nsp8 replicase)|interpro:IPR037230|interpro:IPR014822(Nsp9 replicase)|interpro:IPR036499|interpro:IPR027417|interpro:IPR011050(Pectin lyase fold/virulence factor)|interpro:IPR008740(Peptidase C30, Coronavirus endopeptidase)|interpro:IPR013016(Peptidase C30/C16)|interpro:IPR009003(Peptidase, trypsin-like serine and cysteine)|interpro:IPR009469(RNA polymerase, N-terminal, coronaviral)|interpro:IPR018995|interpro:IPR029063|interpro:IPR027352|interpro:IPR043174|interpro:IPR043472|interpro:IPR043477|interpro:IPR043502|interpro:IPR043504|interpro:IPR044309|interpro:IPR044313|interpro:IPR044322|interpro:IPR044330|interpro:IPR044343|interpro:IPR044353|interpro:IPR044356|interpro:IPR044369|interpro:IPR044371|interpro:IPR044385|interpro:IPR044401 go:"GO:0004197"(cysteine-type endopeptidase activity) chain-seq-start:2966|chain-seq-end:3267 - figure legend:Supp. figure 1A, number 2|comment:PEDV Nsp 7+8 and HCoV-229E Nsp5.|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2020/10/05 2020/10/08 rogid:P7IJ5gWxHpiPJos9YTv1FcuBUH011137 rogid:6ZuJeRKspxH4Qh5SnbbcjiDXzOE229032 rigid:DOKJG/QguRBaV1Y7lnAR32iiG3k false maltose binding protein tag:n-n|his tag:c-c his tag:c-c|sufficient binding region:3580-3857 - - psi-mi:"MI:0816"(molecular weight estimation by staining) psi-mi:"MI:0816"(molecular weight estimation by staining) uniprotkb:Q98VG9-PRO_5000140212 uniprotkb:P0C6Y5 intact:EBI-26366766 intact:EBI-25684285|uniprotkb:Q88508|uniprotkb:Q9IW05|uniprotkb:Q9IW06 psi-mi:q98vg9-pro_5000140212(display_long)|uniprotkb:rep(gene name)|psi-mi:rep(display_short)|uniprotkb:1a-1b(orf name)|uniprotkb:ORF1ab polyprotein(gene name synonym) psi-mi:r1ab_cvppu(display_long)|uniprotkb:rep(gene name)|psi-mi:rep(display_short)|uniprotkb:ORF1ab polyprotein(gene name synonym)|uniprotkb:1a-1b(orf name) psi-mi:"MI:0435"(protease assay) Krichel et al. (2020) doi:10.1101/2020.09.30.320762|imex:IM-28416|pubmed:33024972 taxid:33734(FCoV)|taxid:33734("Feline coronavirus (strain FIPV WSU-79/1146)") taxid:11151(TGEV)|taxid:11151("Porcine transmissible gastroenteritis coronavirus (strain Purdue)") psi-mi:"MI:0570"(protein cleavage) psi-mi:"MI:0469"(IntAct) intact:EBI-26366753|imex:IM-28416-15 - - psi-mi:"MI:0501"(enzyme) psi-mi:"MI:0502"(enzyme target) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) intact:EBI-25757479(chain-parent) go:"GO:0003678"(DNA helicase activity)|go:"GO:0003723"(RNA binding)|go:"GO:0003724"(RNA helicase activity)|go:"GO:0003968"(RNA-directed 5'-3' RNA polymerase activity)|go:"GO:0004197"(cysteine-type endopeptidase activity)|go:"GO:0004519"(endonuclease activity)|go:"GO:0004532"(exoribonuclease activity)|go:"GO:0004843"(thiol-dependent deubiquitinase)|go:"GO:0005524"(ATP binding)|go:"GO:0006351"(transcription, DNA-templated)|go:"GO:0008168"(methyltransferase activity)|go:"GO:0008270"(zinc ion binding)|go:"GO:0016021"(integral component of membrane)|go:"GO:0019079"(viral genome replication)|go:"GO:0019082"(viral protein processing)|go:"GO:0032259"(methylation)|go:"GO:0033644"(host cell membrane)|go:"GO:0039520"(induction by virus of host autophagy)|go:"GO:0039548"(suppression by virus of host viral-induced cytoplasmic pattern recognition receptor signaling pathway via inhibition of IRF3 activity)|go:"GO:0039648"(modulation by virus of host protein ubiquitination)|go:"GO:0044172"(host cell endoplasmic reticulum-Golgi intermediate compartment)|go:"GO:0044220"(host cell perinuclear region of cytoplasm)|go:"GO:0140603"(obsolete ATP hydrolysis activity)|interpro:IPR043177|interpro:IPR043178|interpro:IPR044357|interpro:IPR044309|interpro:IPR044313|interpro:IPR044322|interpro:IPR044330|interpro:IPR044343|interpro:IPR044353|interpro:IPR044356|interpro:IPR044369|interpro:IPR044371|interpro:IPR044385|interpro:IPR044401|interpro:IPR043472|interpro:IPR043477|interpro:IPR043502|interpro:IPR043504|rcsb pdb:1LVO|rcsb pdb:1P9U|rcsb pdb:2AMP|rcsb pdb:6IVC|interpro:IPR027351|interpro:IPR032039|interpro:IPR038634|interpro:IPR032505|interpro:IPR009461(Coronavirus NSP13)|interpro:IPR027352|interpro:IPR041679|interpro:IPR037227|interpro:IPR002589(Appr-1-p processing)|interpro:IPR036333|interpro:IPR009466(NSP11)|interpro:IPR043174|interpro:IPR042515|interpro:IPR038123|interpro:IPR014828(Nsp7 replicase)|interpro:IPR037204|interpro:IPR014829(Nsp8 replicase)|interpro:IPR037230|interpro:IPR014822(Nsp9 replicase)|interpro:IPR036499|interpro:IPR027417|interpro:IPR008740(Peptidase C30, Coronavirus endopeptidase)|interpro:IPR013016(Peptidase C30/C16)|interpro:IPR009003(Peptidase, trypsin-like serine and cysteine)|interpro:IPR009469(RNA polymerase, N-terminal, coronaviral)|interpro:IPR018995|interpro:IPR029063|interpro:IPR043609|interpro:IPR043608|interpro:IPR043606|interpro:IPR043613|interpro:IPR043615|interpro:IPR043611|interpro:IPR043612|interpro:IPR043610|interpro:IPR001205(RNA-directed RNA polymerase, P3D protein) go:"GO:0004197"(cysteine-type endopeptidase activity) chain-seq-start:2904|chain-seq-end:3205 - figure legend:Supp. figure 1A, number 3|comment:TGEV Nsp 7+8 and FIPV Nsp5.|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2020/10/05 2020/10/08 rogid:7b2MW2xoooIYUxEv4K1ktmVbzrc33734 rogid:DyoiFaccCE00JSwk8MJDVWOw/EU11151 rigid:+R9PnXg6wvyoEbstwRcs3gN7UQI false maltose binding protein tag:n-n|his tag:c-c his tag:c-c|sufficient binding region:3475-3752 - - psi-mi:"MI:0816"(molecular weight estimation by staining) psi-mi:"MI:0816"(molecular weight estimation by staining) uniprotkb:Q98VG9-PRO_5000140212 uniprotkb:Q98VG9 intact:EBI-26366766 intact:EBI-25757479|uniprotkb:Q4U5G1|uniprotkb:Q4U5G2|uniprotkb:Q52PA4 psi-mi:q98vg9-pro_5000140212(display_long)|uniprotkb:rep(gene name)|psi-mi:rep(display_short)|uniprotkb:1a-1b(orf name)|uniprotkb:ORF1ab polyprotein(gene name synonym) psi-mi:r1ab_fipv(display_long)|uniprotkb:rep(gene name)|psi-mi:rep(display_short)|uniprotkb:ORF1ab polyprotein(gene name synonym)|uniprotkb:1a-1b(orf name) psi-mi:"MI:0435"(protease assay) Krichel et al. (2020) doi:10.1101/2020.09.30.320762|imex:IM-28416|pubmed:33024972 taxid:33734(FCoV)|taxid:33734("Feline coronavirus (strain FIPV WSU-79/1146)") taxid:33734(FCoV)|taxid:33734("Feline coronavirus (strain FIPV WSU-79/1146)") psi-mi:"MI:0570"(protein cleavage) psi-mi:"MI:0469"(IntAct) intact:EBI-26366772|imex:IM-28416-51 - - psi-mi:"MI:0501"(enzyme) psi-mi:"MI:0502"(enzyme target) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) intact:EBI-25757479(chain-parent) go:"GO:0003678"(DNA helicase activity)|go:"GO:0003723"(RNA binding)|go:"GO:0003724"(RNA helicase activity)|go:"GO:0003968"(RNA-directed 5'-3' RNA polymerase activity)|go:"GO:0004197"(cysteine-type endopeptidase activity)|go:"GO:0004519"(endonuclease activity)|go:"GO:0004532"(exoribonuclease activity)|go:"GO:0004843"(thiol-dependent deubiquitinase)|go:"GO:0005524"(ATP binding)|go:"GO:0006351"(transcription, DNA-templated)|go:"GO:0008168"(methyltransferase activity)|go:"GO:0008270"(zinc ion binding)|go:"GO:0016021"(integral component of membrane)|go:"GO:0019079"(viral genome replication)|go:"GO:0019082"(viral protein processing)|go:"GO:0032259"(methylation)|go:"GO:0033644"(host cell membrane)|go:"GO:0039520"(induction by virus of host autophagy)|go:"GO:0039548"(suppression by virus of host viral-induced cytoplasmic pattern recognition receptor signaling pathway via inhibition of IRF3 activity)|go:"GO:0039648"(modulation by virus of host protein ubiquitination)|go:"GO:0044172"(host cell endoplasmic reticulum-Golgi intermediate compartment)|go:"GO:0044220"(host cell perinuclear region of cytoplasm)|go:"GO:0140603"(obsolete ATP hydrolysis activity)|rcsb pdb:6LP9|interpro:IPR043177|interpro:IPR043178|interpro:IPR044357|rcsb pdb:3ETI|rcsb pdb:3EW5|rcsb pdb:3GZF|rcsb pdb:3JZT|rcsb pdb:3UB0|rcsb pdb:4ZRO|rcsb pdb:5EU8|interpro:IPR027351|interpro:IPR032039|interpro:IPR038634|interpro:IPR032505|interpro:IPR009461(Coronavirus NSP13)|interpro:IPR027352|interpro:IPR041679|interpro:IPR037227|interpro:IPR002589(Appr-1-p processing)|interpro:IPR036333|interpro:IPR009466(NSP11)|interpro:IPR043174|interpro:IPR042515|interpro:IPR038123|interpro:IPR014828(Nsp7 replicase)|interpro:IPR037204|interpro:IPR014829(Nsp8 replicase)|interpro:IPR037230|interpro:IPR014822(Nsp9 replicase)|interpro:IPR036499|interpro:IPR027417|interpro:IPR008740(Peptidase C30, Coronavirus endopeptidase)|interpro:IPR013016(Peptidase C30/C16)|interpro:IPR009003(Peptidase, trypsin-like serine and cysteine)|interpro:IPR009469(RNA polymerase, N-terminal, coronaviral)|interpro:IPR018995|interpro:IPR029063|interpro:IPR043472|interpro:IPR043477|interpro:IPR043502|interpro:IPR043504|interpro:IPR043609|interpro:IPR043608|interpro:IPR043606|interpro:IPR043613|interpro:IPR043615|interpro:IPR043611|interpro:IPR043612|interpro:IPR043610|interpro:IPR001205(RNA-directed RNA polymerase, P3D protein)|interpro:IPR044309|interpro:IPR044313|interpro:IPR044322|interpro:IPR044330|interpro:IPR044343|interpro:IPR044353|interpro:IPR044356|interpro:IPR044369|interpro:IPR044371|interpro:IPR044385|interpro:IPR044401 go:"GO:0004197"(cysteine-type endopeptidase activity) chain-seq-start:2904|chain-seq-end:3205 - figure legend:Supp. figure 1A, number 4|comment:FIPV Nsp 7+8 and FIPV Nsp5.|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2020/10/05 2020/10/08 rogid:7b2MW2xoooIYUxEv4K1ktmVbzrc33734 rogid:SGKcYseDVzOC1ZqdsJZDXG4Hy0s33734 rigid:jnk50f2+gsEGBFxq5HmqkT/q9hs false maltose binding protein tag:n-n|his tag:c-c his tag:c-c|sufficient binding region:3500-3777 - - psi-mi:"MI:0816"(molecular weight estimation by staining) psi-mi:"MI:0816"(molecular weight estimation by staining) uniprotkb:P0C6X7 uniprotkb:P0C6X7-PRO_0000037312 intact:EBI-7843867|uniprotkb:Q6WGN0|uniprotkb:Q808C0|uniprotkb:P59641|uniprotkb:Q80BV8|uniprotkb:Q80BV7|intact:MINT-1487760|uniprotkb:Q80E51|uniprotkb:Q7T697 intact:EBI-25487250 psi-mi:r1ab_sars(display_long)|uniprotkb:rep(gene name)|psi-mi:rep(display_short)|uniprotkb:1a-1b(orf name)|uniprotkb:ORF1ab polyprotein(gene name synonym) psi-mi:p0c6x7-pro_0000037312(display_long)|uniprotkb:rep(gene name)|psi-mi:rep(display_short)|uniprotkb:ORF1ab polyprotein(gene name synonym)|uniprotkb:1a-1b(orf name) psi-mi:"MI:0435"(protease assay) Krichel et al. (2020) doi:10.1101/2020.09.30.320762|imex:IM-28416|pubmed:33024972 taxid:694009(sars-cov)|taxid:694009(Human SARS coronavirus) taxid:694009(sars-cov)|taxid:694009(Human SARS coronavirus) psi-mi:"MI:0570"(protein cleavage) psi-mi:"MI:0469"(IntAct) intact:EBI-26366785|imex:IM-28416-17 - - psi-mi:"MI:0502"(enzyme target) psi-mi:"MI:0501"(enzyme) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) rcsb pdb:5N19|rcsb pdb:5N5O|interpro:IPR002589(Appr-1-p processing)|interpro:IPR007094(RNA-directed RNA polymerase, positive-strand RNA eukaryotic virus)|interpro:IPR008740(Peptidase C30, Coronavirus endopeptidase)|interpro:IPR009461(Coronavirus NSP13)|interpro:IPR009466(NSP11)|interpro:IPR009469(RNA polymerase, N-terminal, coronaviral)|interpro:IPR013016(Peptidase C30/C16)|rcsb pdb:7LCP|rcsb pdb:7LCQ|rcsb pdb:5F22|rcsb pdb:5C8S|rcsb pdb:5C8T|rcsb pdb:5C8U|rcsb pdb:5E6J|go:"GO:0000175"(3'-5'-exoribonuclease activity)|go:"GO:0001172"(transcription, RNA-templated)|go:"GO:0002151"(G-quadruplex RNA binding)|go:"GO:0003678"(DNA helicase activity)|go:"GO:0003725"(double-stranded RNA binding)|go:"GO:0003727"(single-stranded RNA binding)|go:"GO:0003968"(RNA-directed 5'-3' RNA polymerase activity)|go:"GO:0004197"(cysteine-type endopeptidase activity)|go:"GO:0004386"(helicase activity)|go:"GO:0004482"("mRNA (guanine-N7-)-methyltransferase activity")|go:"GO:0004483"("mRNA (nucleoside-2'-O-)-methyltransferase activity")|go:"GO:0004519"(endonuclease activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005789"(endoplasmic reticulum membrane)|go:"GO:0005829"(cytosol)|go:"GO:0006351"(transcription, DNA-templated)|go:"GO:0006370"(7-methylguanosine mRNA capping)|go:"GO:0008270"(zinc ion binding)|go:"GO:0016021"(integral component of membrane)|go:"GO:0016540"(protein autoprocessing)|go:"GO:0019082"(viral protein processing)|go:"GO:0019083"(viral transcription)|go:"GO:0004843"(thiol-dependent deubiquitinase)|go:"GO:0019785"(ISG15-specific protease activity)|go:"GO:0030430"(host cell cytoplasm)|go:"GO:0032574"(5'-3' RNA helicase activity)|go:"GO:0039501"(suppression by virus of host type I interferon production)|go:"GO:0039502"(suppression by virus of host type I interferon-mediated signaling pathway)|go:"GO:0039520"(induction by virus of host autophagy)|go:"GO:0039547"(suppression by virus of host TRAF activity)|go:"GO:0039548"(suppression by virus of host viral-induced cytoplasmic pattern recognition receptor signaling pathway via inhibition of IRF3 activity)|go:"GO:0039579"(suppression by virus of host ISG15-protein conjugation)|go:"GO:0039595"(induction by virus of catabolism of host mRNA)|go:"GO:0039604"(suppression by virus of host translation)|go:"GO:0039644"(suppression by virus of host NF-kappaB cascade)|go:"GO:0039648"(modulation by virus of host protein ubiquitination)|go:"GO:0039690"(positive stranded viral RNA replication)|go:"GO:0039694"(viral RNA genome replication)|go:"GO:0039714"(cytoplasmic viral factory)|go:"GO:0039722"(suppression by virus of host toll-like receptor signaling pathway)|go:"GO:0042802"(identical protein binding)|go:"GO:0044172"(host cell endoplasmic reticulum-Golgi intermediate compartment)|go:"GO:0044220"(host cell perinuclear region of cytoplasm)|go:"GO:0046983"(protein dimerization activity)|go:"GO:0062243"(double membrane vesicle viral factory outer membrane)|go:"GO:0070536"(protein K63-linked deubiquitination)|go:"GO:0071108"(protein K48-linked deubiquitination)|go:"GO:0080009"(mRNA methylation)|go:"GO:0090503"(RNA phosphodiester bond hydrolysis, exonucleolytic)|go:"GO:0140603"(obsolete ATP hydrolysis activity)|go:"GO:1990380"(Lys48-specific deubiquitinase activity)|go:"GO:2000158"(positive regulation of ubiquitin-specific protease activity)|interpro:IPR032505|interpro:IPR032592|rcsb pdb:4TWW|rcsb pdb:4TWY|rcsb pdb:4WY3|interpro:IPR001205(RNA-directed RNA polymerase, P3D protein)|interpro:IPR043606|interpro:IPR043608|interpro:IPR043609|interpro:IPR043610|interpro:IPR043611|interpro:IPR043612|interpro:IPR043613|interpro:IPR043615|rcsb pdb:6LNQ|interpro:IPR043502|rcsb pdb:5NFY|rcsb pdb:4ZUH|rcsb pdb:5B6O|rcsb pdb:5C5N|rcsb pdb:5C5O|interpro:IPR043472|interpro:IPR043477|interpro:IPR043478|interpro:IPR044315|interpro:IPR044322|interpro:IPR044330|interpro:IPR044343|interpro:IPR044351|interpro:IPR044353|interpro:IPR044357|rcsb pdb:2GA6|rcsb pdb:1WOF|rcsb pdb:2QC2|rcsb pdb:1Z1J|rcsb pdb:2OZK|rcsb pdb:2Z3C|rcsb pdb:2RHB|rcsb pdb:2FAV|interpro:IPR018995|rcsb pdb:2GTB|interpro:IPR014828(Nsp7 replicase)|rcsb pdb:2GDT|interpro:IPR027352|rcsb pdb:2ALV|rcsb pdb:2JZF|rcsb pdb:2QIQ|rcsb pdb:2K87|rcsb pdb:2A5I|rcsb pdb:1UK4|interpro:IPR024375|rcsb pdb:1P9T|rcsb pdb:2D2D|rcsb pdb:3R24|rcsb pdb:2GT7|rcsb pdb:2AJ5|interpro:IPR027351|rcsb pdb:2G1F|rcsb pdb:2FE8|rcsb pdb:2GT8|rcsb pdb:1UK3|rcsb pdb:2H85|rcsb pdb:2BX3|rcsb pdb:1Q1X|rcsb pdb:2BX4|interpro:IPR021590|rcsb pdb:2Z9G|rcsb pdb:2ACF|rcsb pdb:1UK2|rcsb pdb:2GRI|rcsb pdb:2A5K|rcsb pdb:1SXF|rcsb pdb:2PWX|interpro:IPR014829(Nsp8 replicase)|interpro:IPR027417|interpro:IPR024358|rcsb pdb:3D62|rcsb pdb:2Z9J|rcsb pdb:2Z3E|rcsb pdb:2GZ8|rcsb pdb:1O5S|interpro:IPR009003(Peptidase, trypsin-like serine and cysteine)|rcsb pdb:2AMD|rcsb pdb:2Z9K|rcsb pdb:1Q2W|rcsb pdb:2VJ1|rcsb pdb:2H2Z|rcsb pdb:2GX4|rcsb pdb:2AHM|rcsb pdb:1UJ1|rcsb pdb:2V6N|rcsb pdb:2HOB|rcsb pdb:1QZ8|rcsb pdb:2Z3D|rcsb pdb:1PA5|rcsb pdb:3EBN|rcsb pdb:2OP9|rcsb pdb:2DUC|rcsb pdb:2QCY|rcsb pdb:2JZD|rcsb pdb:2Q6G|rcsb pdb:1PUK|rcsb pdb:1UW7|rcsb pdb:2GZ9|rcsb pdb:1YSY|rcsb pdb:1Z1I|rcsb pdb:2JZE|rcsb pdb:2C3S|rcsb pdb:2XYQ|rcsb pdb:2XYV|rcsb pdb:2AMQ|rcsb pdb:3E9S|rcsb pdb:2IDY|rcsb pdb:2Z9L|rcsb pdb:2RNK|rcsb pdb:2A5A|interpro:IPR022733|rcsb pdb:2GZ7|rcsb pdb:2Z94|rcsb pdb:1P76|interpro:IPR014822(Nsp9 replicase)|rcsb pdb:2HSX|rcsb pdb:2FYG|rcsb pdb:2XYR|rcsb pdb:2G9T|interpro:IPR042515|rcsb pdb:6NUR|rcsb pdb:6NUS|interpro:IPR044367|interpro:IPR044371|interpro:IPR044374|interpro:IPR044389|interpro:IPR044401|interpro:IPR029063|interpro:IPR038030|interpro:IPR038083|interpro:IPR038123|interpro:IPR038166|interpro:IPR038400|interpro:IPR036333|interpro:IPR036499|interpro:IPR037204|interpro:IPR037227|interpro:IPR037230|interpro:IPR042570|rcsb pdb:6JYT|interpro:IPR043503|interpro:IPR043504|rcsb pdb:6W79|interpro:IPR043174|interpro:IPR043177|interpro:IPR043178|rcsb pdb:2K7X|reactome:R-HSA-9679504|reactome:R-HSA-9682706|reactome:R-HSA-9682708|reactome:R-HSA-9683439|reactome:R-HSA-9684325|refseq:NP_828849.2|rcsb pdb:6WCO intact:EBI-7843867(chain-parent) go:"GO:0004197"(cysteine-type endopeptidase activity) crc64:E6504CAFDC36BC09|comment:mint|function:"The replicase polyprotein of coronaviruses is a multifunctional protein: it contains the activities necessary for the transcription of negative stranded RNA, leader RNA, subgenomic mRNAs and progeny virion RNA as well as proteinases responsible for the cleavage of the polyprotein into functional products"|function:"The papain-like proteinase (PL-PRO) is responsible for the cleavages located at the N-terminus of replicase polyprotein. Activity of PL-PRO is dependent on zinc"|function:"The main proteinase 3CL-PRO is responsible for the majority of cleavages as it cleaves the C-terminus of replicase polyprotein at 11 sites. Recognizes substrates containing the core sequence [ILMVF]-Q-|-[SGACN]. Inhibited by the substrate-analog Cbz-Val-Asn-Ser-Thr-Leu-Gln-CMK (By similarity). Also contains an ADP-ribose-1''-phosphate (ADRP)-binding function"|function:"The helicase which contains a zinc finger structure displays RNA and DNA duplex-unwinding activities with 5' to 3' polarity. Its ATPase activity is strongly stimulated by poly(U), poly(dT), poly(C), poly(dA), but not by poly(G). Activity of helicase is dependent on magnesium"|function:The exoribonuclease acts on both ssRNA and dsRNA in a 3' to 5' direction|function:Nsp7-nsp8 hexadecamer may possibly confer processivity to the polymerase, maybe by binding to dsRNA or by producing primers utilized by the latter|function:Nsp9 is a ssRNA-binding protein|function:"NendoU is a Mn(2+)-dependent, uridylate-specific enzyme, which leaves 2'-3'-cyclic phosphates 5' to the cleaved bond" chain-seq-start:3241|chain-seq-end:3546 figure legend:Supp. figure 1A, number 5|comment:SARS Nsp 7+8 and SARS Nsp5.|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2020/10/05 2020/10/08 rogid:kCQUYVz2xMpwIvb+ZIpnIrNl9GI694009 rogid:6LbRC/jITHtqNPZLpKst/+pNdOQ694009 rigid:U0FqvHo5cLoHK0LGJI585Kh422Q false his tag:c-c|sufficient binding region:3837-4117 his tag:c-c - - psi-mi:"MI:0816"(molecular weight estimation by staining) psi-mi:"MI:0816"(molecular weight estimation by staining) uniprotkb:P0C6X7-PRO_0000037312 uniprotkb:K9N638 intact:EBI-25487250 intact:EBI-25618448 psi-mi:p0c6x7-pro_0000037312(display_long)|uniprotkb:rep(gene name)|psi-mi:rep(display_short)|uniprotkb:ORF1ab polyprotein(gene name synonym)|uniprotkb:1a-1b(orf name) psi-mi:r1a_mers1(display_long)|uniprotkb:ORF1a polyprotein(gene name synonym)|uniprotkb:1a(orf name) psi-mi:"MI:0435"(protease assay) Krichel et al. (2020) doi:10.1101/2020.09.30.320762|imex:IM-28416|pubmed:33024972 taxid:694009(sars-cov)|taxid:694009(Human SARS coronavirus) taxid:1263720(cvemc)|taxid:1263720("Middle East respiratory syndrome-related coronavirus (isolate United Kingdom/H123990006/2012) (Betacoronavirus England 1)") psi-mi:"MI:0570"(protein cleavage) psi-mi:"MI:0469"(IntAct) intact:EBI-26366798|imex:IM-28416-53 - - psi-mi:"MI:0501"(enzyme) psi-mi:"MI:0502"(enzyme target) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) intact:EBI-7843867(chain-parent) go:"GO:0016740"(transferase activity)|go:"GO:0019079"(viral genome replication)|go:"GO:0019082"(viral protein processing)|go:"GO:0033644"(host cell membrane)|go:"GO:0039502"(suppression by virus of host type I interferon-mediated signaling pathway)|go:"GO:0039520"(induction by virus of host autophagy)|go:"GO:0002151"(G-quadruplex RNA binding)|go:"GO:0039548"(suppression by virus of host viral-induced cytoplasmic pattern recognition receptor signaling pathway via inhibition of IRF3 activity)|go:"GO:0003727"(single-stranded RNA binding)|go:"GO:0004197"(cysteine-type endopeptidase activity)|go:"GO:0004843"(thiol-dependent deubiquitinase)|go:"GO:0008270"(zinc ion binding)|go:"GO:0016021"(integral component of membrane)|go:"GO:0039579"(suppression by virus of host ISG15-protein conjugation)|go:"GO:0039595"(induction by virus of catabolism of host mRNA)|go:"GO:0039648"(modulation by virus of host protein ubiquitination)|go:"GO:0044220"(host cell perinuclear region of cytoplasm)|go:"GO:0140603"(obsolete ATP hydrolysis activity)|interpro:IPR022733|interpro:IPR043613|interpro:IPR043615|interpro:IPR043611|interpro:IPR043612|interpro:IPR043610|interpro:IPR021590|interpro:IPR043177|interpro:IPR043178|interpro:IPR043472|interpro:IPR043477|interpro:IPR043503|interpro:IPR043504|rcsb pdb:4R3D|rcsb pdb:5DUS|rcsb pdb:5HIH|rcsb pdb:5WKJ|rcsb pdb:5WKK|rcsb pdb:5WKL|rcsb pdb:5WKM|interpro:IPR032505|interpro:IPR002589(Appr-1-p processing)|interpro:IPR032592|interpro:IPR042570|interpro:IPR036333|interpro:IPR024375|interpro:IPR038400|interpro:IPR038123|interpro:IPR014828(Nsp7 replicase)|interpro:IPR037204|interpro:IPR014829(Nsp8 replicase)|interpro:IPR037230|interpro:IPR014822(Nsp9 replicase)|interpro:IPR036499|interpro:IPR008740(Peptidase C30, Coronavirus endopeptidase)|interpro:IPR013016(Peptidase C30/C16)|interpro:IPR009003(Peptidase, trypsin-like serine and cysteine)|interpro:IPR038083|interpro:IPR018995|interpro:IPR044353|interpro:IPR044357|interpro:IPR044367|interpro:IPR044371|interpro:IPR044382|interpro:IPR044388 go:"GO:0004197"(cysteine-type endopeptidase activity) chain-seq-start:3241|chain-seq-end:3546 - figure legend:Supp. figure 1B|comment:MERS Nsp 7+8+9 and SARS Nsp5.|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2020/10/05 2020/10/08 rogid:6LbRC/jITHtqNPZLpKst/+pNdOQ694009 rogid:L2obGMPmkeM8YgjxyE2006usAhI1263720 rigid:gejwk4xxIqXjyDUDPMYVG/SDpRg false his tag:c-c his tag:c-c|sufficient binding region:3846-4237 - - psi-mi:"MI:0816"(molecular weight estimation by staining) psi-mi:"MI:0816"(molecular weight estimation by staining) uniprotkb:P0C6X7-PRO_0000037312 uniprotkb:K9N638 intact:EBI-25487250 intact:EBI-25618448 psi-mi:p0c6x7-pro_0000037312(display_long)|uniprotkb:rep(gene name)|psi-mi:rep(display_short)|uniprotkb:ORF1ab polyprotein(gene name synonym)|uniprotkb:1a-1b(orf name) psi-mi:r1a_mers1(display_long)|uniprotkb:ORF1a polyprotein(gene name synonym)|uniprotkb:1a(orf name) psi-mi:"MI:0435"(protease assay) Krichel et al. (2020) doi:10.1101/2020.09.30.320762|imex:IM-28416|pubmed:33024972 taxid:694009(sars-cov)|taxid:694009(Human SARS coronavirus) taxid:1263720(cvemc)|taxid:1263720("Middle East respiratory syndrome-related coronavirus (isolate United Kingdom/H123990006/2012) (Betacoronavirus England 1)") psi-mi:"MI:0570"(protein cleavage) psi-mi:"MI:0469"(IntAct) intact:EBI-26366809|imex:IM-28416-55 - - psi-mi:"MI:0501"(enzyme) psi-mi:"MI:0502"(enzyme target) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) intact:EBI-7843867(chain-parent) go:"GO:0016740"(transferase activity)|go:"GO:0019079"(viral genome replication)|go:"GO:0019082"(viral protein processing)|go:"GO:0033644"(host cell membrane)|go:"GO:0039502"(suppression by virus of host type I interferon-mediated signaling pathway)|go:"GO:0039520"(induction by virus of host autophagy)|go:"GO:0002151"(G-quadruplex RNA binding)|go:"GO:0039548"(suppression by virus of host viral-induced cytoplasmic pattern recognition receptor signaling pathway via inhibition of IRF3 activity)|go:"GO:0003727"(single-stranded RNA binding)|go:"GO:0004197"(cysteine-type endopeptidase activity)|go:"GO:0004843"(thiol-dependent deubiquitinase)|go:"GO:0008270"(zinc ion binding)|go:"GO:0016021"(integral component of membrane)|go:"GO:0039579"(suppression by virus of host ISG15-protein conjugation)|go:"GO:0039595"(induction by virus of catabolism of host mRNA)|go:"GO:0039648"(modulation by virus of host protein ubiquitination)|go:"GO:0044220"(host cell perinuclear region of cytoplasm)|go:"GO:0140603"(obsolete ATP hydrolysis activity)|interpro:IPR022733|interpro:IPR043613|interpro:IPR043615|interpro:IPR043611|interpro:IPR043612|interpro:IPR043610|interpro:IPR021590|interpro:IPR043177|interpro:IPR043178|interpro:IPR043472|interpro:IPR043477|interpro:IPR043503|interpro:IPR043504|rcsb pdb:4R3D|rcsb pdb:5DUS|rcsb pdb:5HIH|rcsb pdb:5WKJ|rcsb pdb:5WKK|rcsb pdb:5WKL|rcsb pdb:5WKM|interpro:IPR032505|interpro:IPR002589(Appr-1-p processing)|interpro:IPR032592|interpro:IPR042570|interpro:IPR036333|interpro:IPR024375|interpro:IPR038400|interpro:IPR038123|interpro:IPR014828(Nsp7 replicase)|interpro:IPR037204|interpro:IPR014829(Nsp8 replicase)|interpro:IPR037230|interpro:IPR014822(Nsp9 replicase)|interpro:IPR036499|interpro:IPR008740(Peptidase C30, Coronavirus endopeptidase)|interpro:IPR013016(Peptidase C30/C16)|interpro:IPR009003(Peptidase, trypsin-like serine and cysteine)|interpro:IPR038083|interpro:IPR018995|interpro:IPR044353|interpro:IPR044357|interpro:IPR044367|interpro:IPR044371|interpro:IPR044382|interpro:IPR044388 go:"GO:0004197"(cysteine-type endopeptidase activity) chain-seq-start:3241|chain-seq-end:3546 - figure legend:Supp. figure 1C|comment:MERS Nsps 7-11 and SARS Nsp5.|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2020/10/05 2020/10/08 rogid:6LbRC/jITHtqNPZLpKst/+pNdOQ694009 rogid:L2obGMPmkeM8YgjxyE2006usAhI1263720 rigid:gejwk4xxIqXjyDUDPMYVG/SDpRg false his tag:c-c his tag:c-c|sufficient binding region:3846-4385 - - psi-mi:"MI:0816"(molecular weight estimation by staining) psi-mi:"MI:0816"(molecular weight estimation by staining) uniprotkb:P0C6X1-PRO_0000037300 uniprotkb:P0C6X1-PRO_0000037299 intact:EBI-26366585 intact:EBI-26366570 psi-mi:p0c6x1-pro_0000037300(display_long)|uniprotkb:rep(gene name)|psi-mi:rep(display_short)|uniprotkb:ORF1ab polyprotein(gene name synonym)|uniprotkb:1a-1b(orf name) psi-mi:p0c6x1-pro_0000037299(display_long)|uniprotkb:rep(gene name)|psi-mi:rep(display_short)|uniprotkb:1a-1b(orf name)|uniprotkb:ORF1ab polyprotein(gene name synonym) psi-mi:"MI:0030"(cross-linking study) Krichel et al. (2020) doi:10.1101/2020.09.30.320762|imex:IM-28416|pubmed:33024972 taxid:11137(hcov-229e)|taxid:11137(Human coronavirus 229E) taxid:11137(hcov-229e)|taxid:11137(Human coronavirus 229E) psi-mi:"MI:0407"(direct interaction) psi-mi:"MI:0469"(IntAct) intact:EBI-26366825|imex:IM-28416-35 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) intact:EBI-25637276(chain-parent) intact:EBI-25637276(chain-parent) - chain-seq-start:3630|chain-seq-end:3824 chain-seq-start:3547|chain-seq-end:3629 figure legend:Supp. figure 11B,12.|comment:The main complexes seen for HCoV-22E were nsp7+8 heterodimers, heterotrimers and tetramers. Complexes with multiple stoichiometries were also identified.|comment:229E Nsp7+8|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2020/10/05 2020/10/08 rogid:gxlb75DWNpongHRQRNBPiXJXcsY11137 rogid:sz9PBIP0Z69WOYwozS+EX/AiC3s11137 rigid:8R29z20QhXlF7rF4XlK13rk6+kk false his tag:c-c - - - psi-mi:"MI:0816"(molecular weight estimation by staining)|psi-mi:"MI:0427"(Identification by mass spectrometry) psi-mi:"MI:0816"(molecular weight estimation by staining)|psi-mi:"MI:0427"(Identification by mass spectrometry) uniprotkb:Q98VG9-PRO_5000140215 uniprotkb:Q98VG9-PRO_5000140214 intact:EBI-26365785 intact:EBI-26365769 psi-mi:q98vg9-pro_5000140215(display_long)|uniprotkb:rep(gene name)|psi-mi:rep(display_short)|uniprotkb:ORF1ab polyprotein(gene name synonym)|uniprotkb:1a-1b(orf name) psi-mi:q98vg9-pro_5000140214(display_long)|uniprotkb:rep(gene name)|psi-mi:rep(display_short)|uniprotkb:ORF1ab polyprotein(gene name synonym)|uniprotkb:1a-1b(orf name) psi-mi:"MI:0030"(cross-linking study) Krichel et al. (2020) doi:10.1101/2020.09.30.320762|imex:IM-28416|pubmed:33024972 taxid:33734(FCoV)|taxid:33734("Feline coronavirus (strain FIPV WSU-79/1146)") taxid:33734(FCoV)|taxid:33734("Feline coronavirus (strain FIPV WSU-79/1146)") psi-mi:"MI:0407"(direct interaction) psi-mi:"MI:0469"(IntAct) intact:EBI-26366839|imex:IM-28416-57 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) intact:EBI-25757479(chain-parent) intact:EBI-25757479(chain-parent) - chain-seq-start:3583|chain-seq-end:3777 chain-seq-start:3500|chain-seq-end:3582 figure legend:Supp. figure 11A,12|comment:"FIPV (feline intestine peritonitis virus) Nsp7+8."|comment:The main complexes seen for FIPV were nsp7+8 heterodimers and trimers. Complexes with multiple stoichiometries were also identified.|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2020/10/05 2020/10/08 rogid:sbTkA3Km/7LgbeDURkgcDhLdIoQ33734 rogid:v5VgY9zTRbKwnOMtQdpluMA1PaA33734 rigid:6Ica4Ur6pxdAIdocLhTa5lpGUzo false his tag:c-c - - - psi-mi:"MI:0816"(molecular weight estimation by staining) psi-mi:"MI:0816"(molecular weight estimation by staining) uniprotkb:P0C6X7-PRO_0000037315 uniprotkb:P0C6X7-PRO_0000037314 intact:EBI-25487941 intact:EBI-25487672 psi-mi:p0c6x7-pro_0000037315(display_long)|uniprotkb:ORF1ab polyprotein(gene name synonym)|uniprotkb:1a-1b(orf name)|uniprotkb:rep(gene name)|psi-mi:rep(display_short) psi-mi:p0c6x7-pro_0000037314(display_long)|uniprotkb:rep(gene name)|psi-mi:rep(display_short)|uniprotkb:ORF1ab polyprotein(gene name synonym)|uniprotkb:1a-1b(orf name) psi-mi:"MI:0030"(cross-linking study) Krichel et al. (2020) doi:10.1101/2020.09.30.320762|imex:IM-28416|pubmed:33024972 taxid:694009(sars-cov)|taxid:694009(Human SARS coronavirus) taxid:694009(sars-cov)|taxid:694009(Human SARS coronavirus) psi-mi:"MI:0407"(direct interaction) psi-mi:"MI:0469"(IntAct) intact:EBI-26366848|imex:IM-28416-37 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) intact:EBI-7843867(chain-parent)|uniprotkb:P0C6X7(chain-parent) intact:EBI-7843867(chain-parent)|uniprotkb:P0C6X7(chain-parent) - chain-seq-start:3920|chain-seq-end:4117 chain-seq-start:3837|chain-seq-end:3919 figure legend:Supp. figure 12|comment:Bands are stated to be assigned to SARS-CoV nsp7+8 heterodimers and tetramers.|comment:SARS Nsp7+8|dataset:Virus - Publications including interactions involving viral proteins|dataset:Coronavirus - Interactions investigated in the context of Coronavirus|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2020/10/05 2020/10/08 rogid:3oIsD98B0aiO0Sqlv5jCglM4pgE694009 rogid:95nEGuIgfxXAK4CVWXC+sH1eT+c694009 rigid:UBnYvNAtbcYEdfk5a9xcjeK2wxY false his tag:c-c - 2 2 psi-mi:"MI:0816"(molecular weight estimation by staining) psi-mi:"MI:0816"(molecular weight estimation by staining)