#ID(s) interactor A	ID(s) interactor B	Alt. ID(s) interactor A	Alt. ID(s) interactor B	Alias(es) interactor A	Alias(es) interactor B	Interaction detection method(s)	Publication 1st author(s)	Publication Identifier(s)	Taxid interactor A	Taxid interactor B	Interaction type(s)	Source database(s)	Interaction identifier(s)	Confidence value(s)	Expansion method(s)	Biological role(s) interactor A	Biological role(s) interactor B	Experimental role(s) interactor A	Experimental role(s) interactor B	Type(s) interactor A	Type(s) interactor B	Xref(s) interactor A	Xref(s) interactor B	Interaction Xref(s)	Annotation(s) interactor A	Annotation(s) interactor B	Interaction annotation(s)	Host organism(s)	Interaction parameter(s)	Creation date	Update date	Checksum(s) interactor A	Checksum(s) interactor B	Interaction Checksum(s)	Negative	Feature(s) interactor A	Feature(s) interactor B	Stoichiometry(s) interactor A	Stoichiometry(s) interactor B	Identification method participant A	Identification method participant B
uniprotkb:P83110	uniprotkb:P02666	intact:EBI-2867394|uniprotkb:Q7Z7A2|ensembl:ENSP00000303766	intact:EBI-5260183|uniprotkb:A1YQZ8|uniprotkb:A6N8V0|uniprotkb:Q2TA13|uniprotkb:Q5EEQ6|uniprotkb:Q5EEQ7|uniprotkb:Q6UN63|uniprotkb:Q9BDG5|uniprotkb:Q9TSD5	psi-mi:htra3_human(display_long)|uniprotkb:HTRA3(gene name)|psi-mi:HTRA3(display_short)|uniprotkb:PRSP(gene name synonym)|uniprotkb:High-temperature requirement factor A3(gene name synonym)|uniprotkb:Pregnancy-related serine protease(gene name synonym)	psi-mi:casb_bovin(display_long)|uniprotkb:CSN2(gene name)|psi-mi:CSN2(display_short)	psi-mi:"MI:0435"(protease assay)	Acharya et al. (2020)	pubmed:31899476|imex:IM-27785	taxid:9606(human)|taxid:9606(Homo sapiens)	taxid:9913(bovin)|taxid:9913("Bos taurus (Bovine)")	psi-mi:"MI:0570"(protein cleavage)	psi-mi:"MI:0469"(IntAct)	intact:EBI-25468952|imex:IM-27785-1	-	-	psi-mi:"MI:0501"(enzyme)	psi-mi:"MI:0502"(enzyme target)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_001284488.1|refseq:NP_444272.1|ensembl:ENSG00000170801(gene)|ensembl:ENST00000307358(transcript)|go:"GO:0004175"(endopeptidase activity)|go:"GO:0004252"(serine-type endopeptidase activity)|go:"GO:0005520"(insulin-like growth factor binding)|go:"GO:0005576"(extracellular region)|go:"GO:0006508"(proteolysis)|go:"GO:0008236"(serine-type peptidase activity)|go:"GO:0030512"(negative regulation of transforming growth factor beta receptor signaling pathway)|go:"GO:0030514"(negative regulation of BMP signaling pathway)|go:"GO:0042802"(identical protein binding)|interpro:IPR000867(Insulin-like growth factor-binding protein, IGFBP)|interpro:IPR001478(PDZ/DHR/GLGF)|interpro:IPR001940(Peptidase S1C, HrtA/DegP2/Q/S)|interpro:IPR002350(Proteinase inhibitor I1, Kazal)|interpro:IPR009003(Peptidase, trypsin-like serine and cysteine)|interpro:IPR009030(Growth factor, receptor)|interpro:IPR036034|interpro:IPR036058|interpro:IPR041489|rcsb pdb:2P3W|rcsb pdb:4RI0	dip:DIP-46257N|go:"GO:0004869"(cysteine-type endopeptidase inhibitor activity)|go:"GO:0005615"(extracellular space)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005796"(Golgi lumen)|go:"GO:0008191"(metalloendopeptidase inhibitor activity)|go:"GO:0008217"(regulation of blood pressure)|go:"GO:0009408"(response to heat)|go:"GO:0010804"(negative regulation of tumor necrosis factor-mediated signaling pathway)|go:"GO:0016209"(antioxidant activity)|go:"GO:0019870"(potassium channel inhibitor activity)|go:"GO:0032355"(response to estradiol)|go:"GO:0032570"(response to progesterone)|go:"GO:0042803"(protein homodimerization activity)|go:"GO:0043124"(negative regulation of I-kappaB kinase/NF-kappaB signaling)|go:"GO:0050728"(negative regulation of inflammatory response)|go:"GO:1903488"(negative regulation of lactation)|go:"GO:1903494"(response to dehydroepiandrosterone)|go:"GO:1903496"(response to 11-deoxycorticosterone)|go:"GO:1903720"(negative regulation of I-kappaB phosphorylation)|go:"GO:2000117"(negative regulation of cysteine-type endopeptidase activity)|interpro:IPR001588(Casein, alpha/beta)|interpro:IPR016345(Casein, beta)|interpro:IPR031305	-	-	-	figure legend:3A, Table 2.|comment:HTRA3 may have an additional C terminal His-Tag.|kinetics:"The Vmax, kcat and kcat/K0.5 values obtained for this variant were significantly less than for the wild-type mature HtrA3 (130-453)."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2020/03/11	2020/03/12	rogid:oJfuPj+xPZ+WkCu+VlJeqJAr/3k9606	rogid:wtuOGN4l9NtBjB0YyHhilo7usl49913	rigid:9N/ict3REurhr/o2Qyinh9oGd88	false	his tag:n-n|sufficient binding region:130-340	fluorescein label:?-?	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
uniprotkb:P83110	uniprotkb:P83110	intact:EBI-2867394|uniprotkb:Q7Z7A2|ensembl:ENSP00000303766	intact:EBI-2867394|uniprotkb:Q7Z7A2|ensembl:ENSP00000303766	psi-mi:htra3_human(display_long)|uniprotkb:HTRA3(gene name)|psi-mi:HTRA3(display_short)|uniprotkb:PRSP(gene name synonym)|uniprotkb:High-temperature requirement factor A3(gene name synonym)|uniprotkb:Pregnancy-related serine protease(gene name synonym)	psi-mi:htra3_human(display_long)|uniprotkb:HTRA3(gene name)|psi-mi:HTRA3(display_short)|uniprotkb:PRSP(gene name synonym)|uniprotkb:High-temperature requirement factor A3(gene name synonym)|uniprotkb:Pregnancy-related serine protease(gene name synonym)	psi-mi:"MI:0071"(molecular sieving)	Acharya et al. (2020)	pubmed:31899476|imex:IM-27785	taxid:9606(human)|taxid:9606(Homo sapiens)	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-25468971|imex:IM-27785-3	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_001284488.1|refseq:NP_444272.1|ensembl:ENSG00000170801(gene)|ensembl:ENST00000307358(transcript)|go:"GO:0004175"(endopeptidase activity)|go:"GO:0004252"(serine-type endopeptidase activity)|go:"GO:0005520"(insulin-like growth factor binding)|go:"GO:0005576"(extracellular region)|go:"GO:0006508"(proteolysis)|go:"GO:0008236"(serine-type peptidase activity)|go:"GO:0030512"(negative regulation of transforming growth factor beta receptor signaling pathway)|go:"GO:0030514"(negative regulation of BMP signaling pathway)|go:"GO:0042802"(identical protein binding)|interpro:IPR000867(Insulin-like growth factor-binding protein, IGFBP)|interpro:IPR001478(PDZ/DHR/GLGF)|interpro:IPR001940(Peptidase S1C, HrtA/DegP2/Q/S)|interpro:IPR002350(Proteinase inhibitor I1, Kazal)|interpro:IPR009003(Peptidase, trypsin-like serine and cysteine)|interpro:IPR009030(Growth factor, receptor)|interpro:IPR036034|interpro:IPR036058|interpro:IPR041489|rcsb pdb:2P3W|rcsb pdb:4RI0	refseq:NP_001284488.1|refseq:NP_444272.1|ensembl:ENSG00000170801(gene)|ensembl:ENST00000307358(transcript)|go:"GO:0004175"(endopeptidase activity)|go:"GO:0004252"(serine-type endopeptidase activity)|go:"GO:0005520"(insulin-like growth factor binding)|go:"GO:0005576"(extracellular region)|go:"GO:0006508"(proteolysis)|go:"GO:0008236"(serine-type peptidase activity)|go:"GO:0030512"(negative regulation of transforming growth factor beta receptor signaling pathway)|go:"GO:0030514"(negative regulation of BMP signaling pathway)|go:"GO:0042802"(identical protein binding)|interpro:IPR000867(Insulin-like growth factor-binding protein, IGFBP)|interpro:IPR001478(PDZ/DHR/GLGF)|interpro:IPR001940(Peptidase S1C, HrtA/DegP2/Q/S)|interpro:IPR002350(Proteinase inhibitor I1, Kazal)|interpro:IPR009003(Peptidase, trypsin-like serine and cysteine)|interpro:IPR009030(Growth factor, receptor)|interpro:IPR036034|interpro:IPR036058|interpro:IPR041489|rcsb pdb:2P3W|rcsb pdb:4RI0	-	-	-	figure legend:Supp. Figure 1, Table 3.|comment:HTRA3 may have an additional C-terminal His tag.|comment:"HtrA3 F142A exists as a mixed population of trimers (~30%) and monomers (~70%)."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	-	2020/03/11	2020/03/12	rogid:oJfuPj+xPZ+WkCu+VlJeqJAr/3k9606	rogid:oJfuPj+xPZ+WkCu+VlJeqJAr/3k9606	rigid:3y27DzEz3/YME5lQ6ErWIKXm1r8	false	mutation with no effect:305-305|necessary binding region:175-444|mutation disrupting interaction strength:255-255|mutation disrupting interaction strength:142-142|sufficient binding region:130-453|his tag:n-n|sufficient binding region:359-444	mutation with no effect:305-305|necessary binding region:175-444|mutation disrupting interaction strength:255-255|mutation disrupting interaction strength:142-142|sufficient binding region:130-453|his tag:n-n|sufficient binding region:359-444	3	0	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
uniprotkb:P83110	uniprotkb:P02666	intact:EBI-2867394|uniprotkb:Q7Z7A2|ensembl:ENSP00000303766	intact:EBI-5260183|uniprotkb:A1YQZ8|uniprotkb:A6N8V0|uniprotkb:Q2TA13|uniprotkb:Q5EEQ6|uniprotkb:Q5EEQ7|uniprotkb:Q6UN63|uniprotkb:Q9BDG5|uniprotkb:Q9TSD5	psi-mi:htra3_human(display_long)|uniprotkb:HTRA3(gene name)|psi-mi:HTRA3(display_short)|uniprotkb:PRSP(gene name synonym)|uniprotkb:High-temperature requirement factor A3(gene name synonym)|uniprotkb:Pregnancy-related serine protease(gene name synonym)	psi-mi:casb_bovin(display_long)|uniprotkb:CSN2(gene name)|psi-mi:CSN2(display_short)	psi-mi:"MI:0435"(protease assay)	Acharya et al. (2020)	pubmed:31899476|imex:IM-27785	taxid:9606(human)|taxid:9606(Homo sapiens)	taxid:9913(bovin)|taxid:9913("Bos taurus (Bovine)")	psi-mi:"MI:0570"(protein cleavage)	psi-mi:"MI:0469"(IntAct)	intact:EBI-25469000|imex:IM-27785-5	-	-	psi-mi:"MI:0501"(enzyme)	psi-mi:"MI:0502"(enzyme target)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_001284488.1|refseq:NP_444272.1|ensembl:ENSG00000170801(gene)|ensembl:ENST00000307358(transcript)|go:"GO:0004175"(endopeptidase activity)|go:"GO:0004252"(serine-type endopeptidase activity)|go:"GO:0005520"(insulin-like growth factor binding)|go:"GO:0005576"(extracellular region)|go:"GO:0006508"(proteolysis)|go:"GO:0008236"(serine-type peptidase activity)|go:"GO:0030512"(negative regulation of transforming growth factor beta receptor signaling pathway)|go:"GO:0030514"(negative regulation of BMP signaling pathway)|go:"GO:0042802"(identical protein binding)|interpro:IPR000867(Insulin-like growth factor-binding protein, IGFBP)|interpro:IPR001478(PDZ/DHR/GLGF)|interpro:IPR001940(Peptidase S1C, HrtA/DegP2/Q/S)|interpro:IPR002350(Proteinase inhibitor I1, Kazal)|interpro:IPR009003(Peptidase, trypsin-like serine and cysteine)|interpro:IPR009030(Growth factor, receptor)|interpro:IPR036034|interpro:IPR036058|interpro:IPR041489|rcsb pdb:2P3W|rcsb pdb:4RI0	dip:DIP-46257N|go:"GO:0004869"(cysteine-type endopeptidase inhibitor activity)|go:"GO:0005615"(extracellular space)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005796"(Golgi lumen)|go:"GO:0008191"(metalloendopeptidase inhibitor activity)|go:"GO:0008217"(regulation of blood pressure)|go:"GO:0009408"(response to heat)|go:"GO:0010804"(negative regulation of tumor necrosis factor-mediated signaling pathway)|go:"GO:0016209"(antioxidant activity)|go:"GO:0019870"(potassium channel inhibitor activity)|go:"GO:0032355"(response to estradiol)|go:"GO:0032570"(response to progesterone)|go:"GO:0042803"(protein homodimerization activity)|go:"GO:0043124"(negative regulation of I-kappaB kinase/NF-kappaB signaling)|go:"GO:0050728"(negative regulation of inflammatory response)|go:"GO:1903488"(negative regulation of lactation)|go:"GO:1903494"(response to dehydroepiandrosterone)|go:"GO:1903496"(response to 11-deoxycorticosterone)|go:"GO:1903720"(negative regulation of I-kappaB phosphorylation)|go:"GO:2000117"(negative regulation of cysteine-type endopeptidase activity)|interpro:IPR001588(Casein, alpha/beta)|interpro:IPR016345(Casein, beta)|interpro:IPR031305	-	-	-	figure legend:Figure 4, 7, Supp. figure 3.|comment:HTRA3 may have an additional C terminal His-Tag.|kinetics:"The Vmax, kcat and kcat/K0.5 values obtained for this variant were significantly less than for the wild-type mature HtrA3 (130-453)."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2020/03/11	2020/03/12	rogid:oJfuPj+xPZ+WkCu+VlJeqJAr/3k9606	rogid:wtuOGN4l9NtBjB0YyHhilo7usl49913	rigid:9N/ict3REurhr/o2Qyinh9oGd88	false	mutation disrupting interaction rate:255-255|mutation disrupting interaction rate:305-305|his tag:n-n|sufficient binding region:130-340|necessary binding region:144-174|mutation disrupting interaction rate:142-142|mutation disrupting interaction rate:142-142	fluorescein label:?-?	-	-	psi-mi:"MI:0818"(molecular weight estimation by coomasie staining)	psi-mi:"MI:0818"(molecular weight estimation by coomasie staining)
uniprotkb:P83110	uniprotkb:P02666	intact:EBI-2867394|uniprotkb:Q7Z7A2|ensembl:ENSP00000303766	intact:EBI-5260183|uniprotkb:A1YQZ8|uniprotkb:A6N8V0|uniprotkb:Q2TA13|uniprotkb:Q5EEQ6|uniprotkb:Q5EEQ7|uniprotkb:Q6UN63|uniprotkb:Q9BDG5|uniprotkb:Q9TSD5	psi-mi:htra3_human(display_long)|uniprotkb:HTRA3(gene name)|psi-mi:HTRA3(display_short)|uniprotkb:PRSP(gene name synonym)|uniprotkb:High-temperature requirement factor A3(gene name synonym)|uniprotkb:Pregnancy-related serine protease(gene name synonym)	psi-mi:casb_bovin(display_long)|uniprotkb:CSN2(gene name)|psi-mi:CSN2(display_short)	psi-mi:"MI:0435"(protease assay)	Acharya et al. (2020)	pubmed:31899476|imex:IM-27785	taxid:9606(human)|taxid:9606(Homo sapiens)	taxid:9913(bovin)|taxid:9913("Bos taurus (Bovine)")	psi-mi:"MI:0570"(protein cleavage)	psi-mi:"MI:0469"(IntAct)	intact:EBI-25469033|imex:IM-27785-7	-	-	psi-mi:"MI:0501"(enzyme)	psi-mi:"MI:0502"(enzyme target)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_001284488.1|refseq:NP_444272.1|ensembl:ENSG00000170801(gene)|ensembl:ENST00000307358(transcript)|go:"GO:0004175"(endopeptidase activity)|go:"GO:0004252"(serine-type endopeptidase activity)|go:"GO:0005520"(insulin-like growth factor binding)|go:"GO:0005576"(extracellular region)|go:"GO:0006508"(proteolysis)|go:"GO:0008236"(serine-type peptidase activity)|go:"GO:0030512"(negative regulation of transforming growth factor beta receptor signaling pathway)|go:"GO:0030514"(negative regulation of BMP signaling pathway)|go:"GO:0042802"(identical protein binding)|interpro:IPR000867(Insulin-like growth factor-binding protein, IGFBP)|interpro:IPR001478(PDZ/DHR/GLGF)|interpro:IPR001940(Peptidase S1C, HrtA/DegP2/Q/S)|interpro:IPR002350(Proteinase inhibitor I1, Kazal)|interpro:IPR009003(Peptidase, trypsin-like serine and cysteine)|interpro:IPR009030(Growth factor, receptor)|interpro:IPR036034|interpro:IPR036058|interpro:IPR041489|rcsb pdb:2P3W|rcsb pdb:4RI0	dip:DIP-46257N|go:"GO:0004869"(cysteine-type endopeptidase inhibitor activity)|go:"GO:0005615"(extracellular space)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005796"(Golgi lumen)|go:"GO:0008191"(metalloendopeptidase inhibitor activity)|go:"GO:0008217"(regulation of blood pressure)|go:"GO:0009408"(response to heat)|go:"GO:0010804"(negative regulation of tumor necrosis factor-mediated signaling pathway)|go:"GO:0016209"(antioxidant activity)|go:"GO:0019870"(potassium channel inhibitor activity)|go:"GO:0032355"(response to estradiol)|go:"GO:0032570"(response to progesterone)|go:"GO:0042803"(protein homodimerization activity)|go:"GO:0043124"(negative regulation of I-kappaB kinase/NF-kappaB signaling)|go:"GO:0050728"(negative regulation of inflammatory response)|go:"GO:1903488"(negative regulation of lactation)|go:"GO:1903494"(response to dehydroepiandrosterone)|go:"GO:1903496"(response to 11-deoxycorticosterone)|go:"GO:1903720"(negative regulation of I-kappaB phosphorylation)|go:"GO:2000117"(negative regulation of cysteine-type endopeptidase activity)|interpro:IPR001588(Casein, alpha/beta)|interpro:IPR016345(Casein, beta)|interpro:IPR031305	-	-	-	figure legend:Figure 8, Table 4.|comment:HTRA3 may have an additional C terminal His-Tag.|comment:Cleavage sites identified  are between 15A-16R, 71Q-72S, 138Q-139S and 170V-171M.|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2020/03/11	2020/03/12	rogid:oJfuPj+xPZ+WkCu+VlJeqJAr/3k9606	rogid:wtuOGN4l9NtBjB0YyHhilo7usl49913	rigid:9N/ict3REurhr/o2Qyinh9oGd88	false	his tag:n-n|sufficient binding region:130-453	fluorescein label:?-?	-	-	psi-mi:"MI:0039"(edman degradation)	psi-mi:"MI:0039"(edman degradation)