#ID(s) interactor A	ID(s) interactor B	Alt. ID(s) interactor A	Alt. ID(s) interactor B	Alias(es) interactor A	Alias(es) interactor B	Interaction detection method(s)	Publication 1st author(s)	Publication Identifier(s)	Taxid interactor A	Taxid interactor B	Interaction type(s)	Source database(s)	Interaction identifier(s)	Confidence value(s)	Expansion method(s)	Biological role(s) interactor A	Biological role(s) interactor B	Experimental role(s) interactor A	Experimental role(s) interactor B	Type(s) interactor A	Type(s) interactor B	Xref(s) interactor A	Xref(s) interactor B	Interaction Xref(s)	Annotation(s) interactor A	Annotation(s) interactor B	Interaction annotation(s)	Host organism(s)	Interaction parameter(s)	Creation date	Update date	Checksum(s) interactor A	Checksum(s) interactor B	Interaction Checksum(s)	Negative	Feature(s) interactor A	Feature(s) interactor B	Stoichiometry(s) interactor A	Stoichiometry(s) interactor B	Identification method participant A	Identification method participant B
uniprotkb:P15006	uniprotkb:P15005	intact:EBI-25407271|ensemblbacteria:AAC77301|ensemblbacteria:BAE78335|uniprotkb:Q2M5X1	intact:EBI-552513|uniprotkb:Q2M5X0|ensemblbacteria:AAC77302|ensemblbacteria:BAE78336	psi-mi:mcrc_ecoli(display_long)|uniprotkb:mcrC(gene name)|psi-mi:mcrC(display_short)|uniprotkb:b4345(locus name)|uniprotkb:JW5789(locus name)	psi-mi:mcrb_ecoli(display_long)|uniprotkb:mcrB(gene name)|psi-mi:mcrB(display_short)|uniprotkb:rglB(gene name synonym)|uniprotkb:b4346(locus name)|uniprotkb:EcoKMcrBC(gene name synonym)|uniprotkb:JW5871(locus name)	psi-mi:"MI:0410"(3D electron microscopy)	Nirwan et al. (2019)	imex:IM-27980|pubmed:31296862	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0486"(UniProt)	intact:EBI-25407289|emdb:EMD-0310|wwpdb:6hz6|emdb:EMD-0312|emdb:EMD-0314|wwpdb:6hz5|emdb:EMD-0311|wwpdb:6hz8|wwpdb:6hz9|emdb:EMD-0315|wwpdb:6hz7|emdb:EMD-0313|imex:IM-27980-1|pdbe:6HZ4	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	ensemblbacteria:AAC77301(transcript)|ensemblbacteria:b4345(gene)|ensemblbacteria:BAE78335(gene)|ensemblbacteria:BAE78335(transcript)|go:"GO:0009307"(DNA restriction-modification system)|refseq:NP_418765.1|refseq:WP_000437621.1|rcsb pdb:6HZ4|rcsb pdb:6HZ5|rcsb pdb:6HZ6|rcsb pdb:6HZ7|rcsb pdb:6HZ8|rcsb pdb:6HZ9|interpro:IPR014407(McrBC 5-methylcytosine restriction system component)|interpro:IPR019292|rcsb pdb:6UT6	refseq:NP_418766.4|refseq:WP_000443951.1|dip:DIP-10168N|ensemblbacteria:AAC77302(transcript)|ensemblbacteria:b4346(gene)|ensemblbacteria:BAE78336(gene)|ensemblbacteria:BAE78336(transcript)|go:"GO:0003677"(DNA binding)|go:"GO:0003924"(GTPase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005525"(GTP binding)|go:"GO:0006308"(DNA catabolic process)|go:"GO:0009307"(DNA restriction-modification system)|go:"GO:0015666"(restriction endodeoxyribonuclease activity)|go:"GO:0042802"(identical protein binding)|interpro:IPR003593(AAA+ ATPase, core)|interpro:IPR011704(ATPase associated with various cellular activities, AAA-5)|interpro:IPR021961|interpro:IPR027417|rcsb pdb:3SSC|rcsb pdb:3SSD|rcsb pdb:3SSE|rcsb pdb:6GCD|rcsb pdb:6GCE|rcsb pdb:6GCF|rcsb pdb:6HZ4|rcsb pdb:6HZ5|rcsb pdb:6HZ6|rcsb pdb:6HZ7|rcsb pdb:6HZ8|rcsb pdb:6HZ9|rcsb pdb:6UT6	-	-	-	figure legend:Supp. Table 2|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2020/01/24	2020/01/24	rogid:iprdfSUoc6zM1fW0l48PtpGp8ag83333	rogid:IymvP1zGLI5UVlcomJ6LS7wf9cc83333	rigid:Trcv8keoI7TC0LuWWF4WGxZqgzI	false	-	his tag:c-c|sufficient binding region:162-459	2	12	psi-mi:"MI:2287"(identification by structure determination)	psi-mi:"MI:2287"(identification by structure determination)
uniprotkb:P15005	uniprotkb:P15005	intact:EBI-552513|uniprotkb:Q2M5X0|ensemblbacteria:AAC77302|ensemblbacteria:BAE78336	intact:EBI-552513|uniprotkb:Q2M5X0|ensemblbacteria:AAC77302|ensemblbacteria:BAE78336	psi-mi:mcrb_ecoli(display_long)|uniprotkb:mcrB(gene name)|psi-mi:mcrB(display_short)|uniprotkb:rglB(gene name synonym)|uniprotkb:b4346(locus name)|uniprotkb:EcoKMcrBC(gene name synonym)|uniprotkb:JW5871(locus name)	psi-mi:mcrb_ecoli(display_long)|uniprotkb:mcrB(gene name)|psi-mi:mcrB(display_short)|uniprotkb:rglB(gene name synonym)|uniprotkb:b4346(locus name)|uniprotkb:EcoKMcrBC(gene name synonym)|uniprotkb:JW5871(locus name)	psi-mi:"MI:0071"(molecular sieving)	Nirwan et al. (2019)	imex:IM-27980|pubmed:31296862	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0486"(UniProt)	intact:EBI-25407412|imex:IM-27980-3	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_418766.4|refseq:WP_000443951.1|dip:DIP-10168N|ensemblbacteria:AAC77302(transcript)|ensemblbacteria:b4346(gene)|ensemblbacteria:BAE78336(gene)|ensemblbacteria:BAE78336(transcript)|go:"GO:0003677"(DNA binding)|go:"GO:0003924"(GTPase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005525"(GTP binding)|go:"GO:0006308"(DNA catabolic process)|go:"GO:0009307"(DNA restriction-modification system)|go:"GO:0015666"(restriction endodeoxyribonuclease activity)|go:"GO:0042802"(identical protein binding)|interpro:IPR003593(AAA+ ATPase, core)|interpro:IPR011704(ATPase associated with various cellular activities, AAA-5)|interpro:IPR021961|interpro:IPR027417|rcsb pdb:3SSC|rcsb pdb:3SSD|rcsb pdb:3SSE|rcsb pdb:6GCD|rcsb pdb:6GCE|rcsb pdb:6GCF|rcsb pdb:6HZ4|rcsb pdb:6HZ5|rcsb pdb:6HZ6|rcsb pdb:6HZ7|rcsb pdb:6HZ8|rcsb pdb:6HZ9|rcsb pdb:6UT6	refseq:NP_418766.4|refseq:WP_000443951.1|dip:DIP-10168N|ensemblbacteria:AAC77302(transcript)|ensemblbacteria:b4346(gene)|ensemblbacteria:BAE78336(gene)|ensemblbacteria:BAE78336(transcript)|go:"GO:0003677"(DNA binding)|go:"GO:0003924"(GTPase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005525"(GTP binding)|go:"GO:0006308"(DNA catabolic process)|go:"GO:0009307"(DNA restriction-modification system)|go:"GO:0015666"(restriction endodeoxyribonuclease activity)|go:"GO:0042802"(identical protein binding)|interpro:IPR003593(AAA+ ATPase, core)|interpro:IPR011704(ATPase associated with various cellular activities, AAA-5)|interpro:IPR021961|interpro:IPR027417|rcsb pdb:3SSC|rcsb pdb:3SSD|rcsb pdb:3SSE|rcsb pdb:6GCD|rcsb pdb:6GCE|rcsb pdb:6GCF|rcsb pdb:6HZ4|rcsb pdb:6HZ5|rcsb pdb:6HZ6|rcsb pdb:6HZ7|rcsb pdb:6HZ8|rcsb pdb:6HZ9|rcsb pdb:6UT6	-	-	-	figure legend:Fig. 2G|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2020/01/24	2020/01/24	rogid:IymvP1zGLI5UVlcomJ6LS7wf9cc83333	rogid:IymvP1zGLI5UVlcomJ6LS7wf9cc83333	rigid:s/1vtnc4VLCxpMhuxcmWYyeSwBM	false	his tag:c-c|sufficient binding region:162-459|mutation disrupting interaction strength:348-348|mutation disrupting interaction strength:349-349|mutation with no effect:298-298	his tag:c-c|sufficient binding region:162-459|mutation disrupting interaction strength:348-348|mutation disrupting interaction strength:349-349|mutation with no effect:298-298	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
uniprotkb:P15006	uniprotkb:P15005	intact:EBI-25407271|ensemblbacteria:AAC77301|ensemblbacteria:BAE78335|uniprotkb:Q2M5X1	intact:EBI-552513|uniprotkb:Q2M5X0|ensemblbacteria:AAC77302|ensemblbacteria:BAE78336	psi-mi:mcrc_ecoli(display_long)|uniprotkb:mcrC(gene name)|psi-mi:mcrC(display_short)|uniprotkb:b4345(locus name)|uniprotkb:JW5789(locus name)	psi-mi:mcrb_ecoli(display_long)|uniprotkb:mcrB(gene name)|psi-mi:mcrB(display_short)|uniprotkb:rglB(gene name synonym)|uniprotkb:b4346(locus name)|uniprotkb:EcoKMcrBC(gene name synonym)|uniprotkb:JW5871(locus name)	psi-mi:"MI:0071"(molecular sieving)	Nirwan et al. (2019)	imex:IM-27980|pubmed:31296862	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0486"(UniProt)	intact:EBI-25407477|imex:IM-27980-5	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	ensemblbacteria:AAC77301(transcript)|ensemblbacteria:b4345(gene)|ensemblbacteria:BAE78335(gene)|ensemblbacteria:BAE78335(transcript)|go:"GO:0009307"(DNA restriction-modification system)|refseq:NP_418765.1|refseq:WP_000437621.1|rcsb pdb:6HZ4|rcsb pdb:6HZ5|rcsb pdb:6HZ6|rcsb pdb:6HZ7|rcsb pdb:6HZ8|rcsb pdb:6HZ9|interpro:IPR014407(McrBC 5-methylcytosine restriction system component)|interpro:IPR019292|rcsb pdb:6UT6	refseq:NP_418766.4|refseq:WP_000443951.1|dip:DIP-10168N|ensemblbacteria:AAC77302(transcript)|ensemblbacteria:b4346(gene)|ensemblbacteria:BAE78336(gene)|ensemblbacteria:BAE78336(transcript)|go:"GO:0003677"(DNA binding)|go:"GO:0003924"(GTPase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005525"(GTP binding)|go:"GO:0006308"(DNA catabolic process)|go:"GO:0009307"(DNA restriction-modification system)|go:"GO:0015666"(restriction endodeoxyribonuclease activity)|go:"GO:0042802"(identical protein binding)|interpro:IPR003593(AAA+ ATPase, core)|interpro:IPR011704(ATPase associated with various cellular activities, AAA-5)|interpro:IPR021961|interpro:IPR027417|rcsb pdb:3SSC|rcsb pdb:3SSD|rcsb pdb:3SSE|rcsb pdb:6GCD|rcsb pdb:6GCE|rcsb pdb:6GCF|rcsb pdb:6HZ4|rcsb pdb:6HZ5|rcsb pdb:6HZ6|rcsb pdb:6HZ7|rcsb pdb:6HZ8|rcsb pdb:6HZ9|rcsb pdb:6UT6	-	-	-	figure legend:"Fig. 2G (inset)"|comment:Interaction occurs in the presence of GTP|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2020/01/24	2020/01/24	rogid:iprdfSUoc6zM1fW0l48PtpGp8ag83333	rogid:IymvP1zGLI5UVlcomJ6LS7wf9cc83333	rigid:Trcv8keoI7TC0LuWWF4WGxZqgzI	false	-	his tag:c-c|sufficient binding region:162-459|mutation disrupting interaction strength:348-348|mutation disrupting interaction strength:349-349|mutation with no effect:298-298	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
chebi:"CHEBI:78408"	uniprotkb:P15005	intact:EBI-11175273|chebi:"CHEBI:42862"	intact:EBI-552513|uniprotkb:Q2M5X0|ensemblbacteria:AAC77302|ensemblbacteria:BAE78336	psi-mi:guanosine 5'-[beta,gamma-imido]triphosphate(display_short)|psi-mi:"CHEBI:78408"(display_long)|intact:5'-Guanylic acid, monoanhydride with imidodiphosphoric acid(synonym)|intact:5'-guanylylimidodiphosphate(synonym)|intact:GMP-PNP(synonym)|intact:GMP-Pnp(synonym)|intact:"Gpp(NH)-p"(synonym)|intact:"Gpp(NH)p"(synonym)|intact:Guanylyl imidodiphosphate(synonym)|intact:"5'-O-(hydroxy{[hydroxy(phosphonoamino)phosphoryl]oxy}phosphoryl)guanosine"(iupac name)	psi-mi:mcrb_ecoli(display_long)|uniprotkb:mcrB(gene name)|psi-mi:mcrB(display_short)|uniprotkb:rglB(gene name synonym)|uniprotkb:b4346(locus name)|uniprotkb:EcoKMcrBC(gene name synonym)|uniprotkb:JW5871(locus name)	psi-mi:"MI:0051"(fluorescence technology)	Nirwan et al. (2019)	imex:IM-27980|pubmed:31296862	taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)")	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0486"(UniProt)	intact:EBI-25407509|imex:IM-27980-7	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0328"(small molecule)	psi-mi:"MI:0326"(protein)	-	refseq:NP_418766.4|refseq:WP_000443951.1|dip:DIP-10168N|ensemblbacteria:AAC77302(transcript)|ensemblbacteria:b4346(gene)|ensemblbacteria:BAE78336(gene)|ensemblbacteria:BAE78336(transcript)|go:"GO:0003677"(DNA binding)|go:"GO:0003924"(GTPase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005525"(GTP binding)|go:"GO:0006308"(DNA catabolic process)|go:"GO:0009307"(DNA restriction-modification system)|go:"GO:0015666"(restriction endodeoxyribonuclease activity)|go:"GO:0042802"(identical protein binding)|interpro:IPR003593(AAA+ ATPase, core)|interpro:IPR011704(ATPase associated with various cellular activities, AAA-5)|interpro:IPR021961|interpro:IPR027417|rcsb pdb:3SSC|rcsb pdb:3SSD|rcsb pdb:3SSE|rcsb pdb:6GCD|rcsb pdb:6GCE|rcsb pdb:6GCF|rcsb pdb:6HZ4|rcsb pdb:6HZ5|rcsb pdb:6HZ6|rcsb pdb:6HZ7|rcsb pdb:6HZ8|rcsb pdb:6HZ9|rcsb pdb:6UT6	-	-	-	figure legend:Fig. 2H|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2020/01/24	2020/01/24	-	rogid:IymvP1zGLI5UVlcomJ6LS7wf9cc83333	-	false	fluorescent dye label:?-?	his tag:c-c|sufficient binding region:162-459|mutation disrupting interaction strength:348-348|mutation disrupting interaction strength:349-349|mutation with no effect:298-298	-	-	psi-mi:"MI:0867"(tag visualisation by fluorescence)	psi-mi:"MI:0867"(tag visualisation by fluorescence)
chebi:"CHEBI:15996"	intenz:3.1.21	intact:EBI-989053|chebi:"CHEBI:42934"|chebi:"CHEBI:5234"|chebi:"CHEBI:13342"|chebi:"CHEBI:24451"	intact:EBI-25407354|wwpdb:6hz6|emdb:EMD-0312|emdb:EMD-0314|wwpdb:6hz5|emdb:EMD-0311|wwpdb:6hz4|emdb:EMD-0310|wwpdb:6hz8|wwpdb:6hz9|emdb:EMD-0315|wwpdb:6hz7|emdb:EMD-0313	psi-mi:gtp(display_short)|psi-mi:"CHEBI:15996"(display_long)|intact:5'-GTP(synonym)|intact:GTP(synonym)|intact:Guanosine 5'-triphosphate(synonym)|intact:guanosine 5'-triphosphoric acid(synonym)|intact:guanosine triphosphate(synonym)|intact:GUANOSINE-5'-TRIPHOSPHATE(synonym)|intact:H4gtp(synonym)|intact:"guanosine 5'-(tetrahydrogen triphosphate)"(iupac name)	psi-mi:mcrbc_ecoli(display_short)|psi-mi:3.1.21(display_long)|intact:McrBC 5-methylcytosine-specific restriction endonuclease complex(complex recommended name)|intact:"12xmcrB:2xmcrC"(complex systematic name)	psi-mi:"MI:0419"(gtpase assay)	Nirwan et al. (2019)	imex:IM-27980|pubmed:31296862	taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)")	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	psi-mi:"MI:0883"(gtpase reaction)	psi-mi:"MI:0486"(UniProt)	intact:EBI-25407532|imex:IM-27980-9	-	-	psi-mi:"MI:0502"(enzyme target)	psi-mi:"MI:0501"(enzyme)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0328"(small molecule)	psi-mi:"MI:1302"(stable complex)	-	pubmed:31296862(see-also)|go:"GO:1905348"(endonuclease complex)|go:"GO:0003924"(GTPase activity)|go:"GO:0005525"(GTP binding)|go:"GO:0015666"(restriction endodeoxyribonuclease activity)|go:"GO:0009307"(DNA restriction-modification system)|pubmed:23990325(see-also)|intact:EBI-25407289(exp-evidence)|complex portal:CPX-5285(complex-primary)|evidence ontology:"ECO:0000353"	go:"GO:0003924"(GTPase activity)	author-identifiers:"InChI=1/C10H16N5O14P3/c11-10-13-7-4(8(18)14-10)12-2-15(7)9-6(17)5(16)3(27-9)1-26-31(22,23)29-32(24,25)28-30(19,20)21/h2-3,5-6,9,16-17H,1H2,(H,22,23)(H,24,25)(H2,19,20,21)(H3,11,13,14,18)/t3-,5-,6-,9-/m1/s1/f/h14,19-20,22,24H,11H2"	curated-complex:"A DNA endonuclease that acts as an antiphage defense system by specifically cleaving invading DNA that are methylated. McrBC specifically cleaves DNA containing the recognition sequence 5prime-G/A(5mC)-3prime, where 5mC can be 5-methylcytosine, 5-hydroxymethylcytosine, or 4-methylcytosine."|complex-properties:The AAA+ GTPase motor mcrB is activated by mcrC, which in turn powers DNA cleavage by the endonuclease. Two hexameric rings of mcrB are bridged by an mcrC dimer. The inside of the central channel of the AAA+ mcrB ring shows a circular patch of positively charged residues, suggesting that DNA threads through the central channel.|complex-assembly:Heterotetradecamer	figure legend:Fig. 2I|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2020/01/24	2020/01/24	-	-	-	false	fluorescent dye label:?-?	his tag:c-c|sufficient binding region:162-459|mutation disrupting interaction rate:348-348|mutation disrupting interaction rate:349-349|mutation disrupting interaction rate:298-298	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
intact:EBI-25407584	intact:EBI-25407554	-	-	psi-mi:mcrb_dna-3prime(display_short)|psi-mi:EBI-25407584(display_long)	psi-mi:mcrb_dna-5prime(display_short)|psi-mi:EBI-25407554(display_long)	psi-mi:"MI:1034"(nuclease assay)	Nirwan et al. (2019)	imex:IM-27980|pubmed:31296862	taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)")	taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)")	psi-mi:"MI:0883"(gtpase reaction)	psi-mi:"MI:0486"(UniProt)	intact:EBI-25407562|imex:IM-27980-11	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0502"(enzyme target)	psi-mi:"MI:0502"(enzyme target)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0319"(deoxyribonucleic acid)	psi-mi:"MI:0319"(deoxyribonucleic acid)	-	-	go:"GO:0003924"(GTPase activity)	-	-	figure legend:Fig. 2J|comment:Interaction occurs in the presence of GTP|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2020/01/24	2020/01/24	-	-	-	false	binding-associated region:?-?	binding-associated region:?-?	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
intact:EBI-25407584	intenz:3.1.21	-	intact:EBI-25407354|wwpdb:6hz6|emdb:EMD-0312|emdb:EMD-0314|wwpdb:6hz5|emdb:EMD-0311|wwpdb:6hz4|emdb:EMD-0310|wwpdb:6hz8|wwpdb:6hz9|emdb:EMD-0315|wwpdb:6hz7|emdb:EMD-0313	psi-mi:mcrb_dna-3prime(display_short)|psi-mi:EBI-25407584(display_long)	psi-mi:mcrbc_ecoli(display_short)|psi-mi:3.1.21(display_long)|intact:McrBC 5-methylcytosine-specific restriction endonuclease complex(complex recommended name)|intact:"12xmcrB:2xmcrC"(complex systematic name)	psi-mi:"MI:1034"(nuclease assay)	Nirwan et al. (2019)	imex:IM-27980|pubmed:31296862	taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)")	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	psi-mi:"MI:0883"(gtpase reaction)	psi-mi:"MI:0486"(UniProt)	intact:EBI-25407562|imex:IM-27980-11	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0502"(enzyme target)	psi-mi:"MI:0501"(enzyme)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0319"(deoxyribonucleic acid)	psi-mi:"MI:1302"(stable complex)	-	pubmed:31296862(see-also)|go:"GO:1905348"(endonuclease complex)|go:"GO:0003924"(GTPase activity)|go:"GO:0005525"(GTP binding)|go:"GO:0015666"(restriction endodeoxyribonuclease activity)|go:"GO:0009307"(DNA restriction-modification system)|pubmed:23990325(see-also)|intact:EBI-25407289(exp-evidence)|complex portal:CPX-5285(complex-primary)|evidence ontology:"ECO:0000353"	go:"GO:0003924"(GTPase activity)	-	curated-complex:"A DNA endonuclease that acts as an antiphage defense system by specifically cleaving invading DNA that are methylated. McrBC specifically cleaves DNA containing the recognition sequence 5prime-G/A(5mC)-3prime, where 5mC can be 5-methylcytosine, 5-hydroxymethylcytosine, or 4-methylcytosine."|complex-properties:The AAA+ GTPase motor mcrB is activated by mcrC, which in turn powers DNA cleavage by the endonuclease. Two hexameric rings of mcrB are bridged by an mcrC dimer. The inside of the central channel of the AAA+ mcrB ring shows a circular patch of positively charged residues, suggesting that DNA threads through the central channel.|complex-assembly:Heterotetradecamer	figure legend:Fig. 2J|comment:Interaction occurs in the presence of GTP|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2020/01/24	2020/01/24	-	-	-	false	binding-associated region:?-?	his tag:c-c|sufficient binding region:162-459|mutation disrupting interaction rate:348-348|mutation disrupting interaction rate:349-349|mutation disrupting interaction rate:298-298	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)