#ID(s) interactor A	ID(s) interactor B	Alt. ID(s) interactor A	Alt. ID(s) interactor B	Alias(es) interactor A	Alias(es) interactor B	Interaction detection method(s)	Publication 1st author(s)	Publication Identifier(s)	Taxid interactor A	Taxid interactor B	Interaction type(s)	Source database(s)	Interaction identifier(s)	Confidence value(s)	Expansion method(s)	Biological role(s) interactor A	Biological role(s) interactor B	Experimental role(s) interactor A	Experimental role(s) interactor B	Type(s) interactor A	Type(s) interactor B	Xref(s) interactor A	Xref(s) interactor B	Interaction Xref(s)	Annotation(s) interactor A	Annotation(s) interactor B	Interaction annotation(s)	Host organism(s)	Interaction parameter(s)	Creation date	Update date	Checksum(s) interactor A	Checksum(s) interactor B	Interaction Checksum(s)	Negative	Feature(s) interactor A	Feature(s) interactor B	Stoichiometry(s) interactor A	Stoichiometry(s) interactor B	Identification method participant A	Identification method participant B
uniprotkb:J7M6Z1	uniprotkb:J7M6Z1	intact:EBI-26442404	intact:EBI-26442404	psi-mi:j7m6z1_strp1(display_long)|uniprotkb:covR(gene name)|psi-mi:covR(display_short)|uniprotkb:M1GAS476_0346(orf name)	psi-mi:j7m6z1_strp1(display_long)|uniprotkb:covR(gene name)|psi-mi:covR(display_short)|uniprotkb:M1GAS476_0346(orf name)	psi-mi:"MI:0404"(comigration in non denaturing gel electrophoresis)	Horstmann et al. (2014)	imex:IM-28477|pubmed:24788524	taxid:1207470(Streptococcus pyogenes M1 476)|taxid:1207470(Streptococcus pyogenes M1 476)	taxid:1207470(Streptococcus pyogenes M1 476)|taxid:1207470(Streptococcus pyogenes M1 476)	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0471"(MINT)	intact:EBI-26442400|imex:IM-28477-1	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	go:"GO:0000160"(phosphorelay signal transduction system)|go:"GO:0003677"(DNA binding)|go:"GO:0006355"(regulation of transcription, DNA-templated)|mint:J7M6Z1|refseq:WP_002991052.1|interpro:IPR011006(CheY-like)|interpro:IPR001867(Signal transduction response regulator, C-terminal)|interpro:IPR016032(Signal transduction response regulator, C-terminal effector)|interpro:IPR001789(Signal transduction response regulator, receiver region)|interpro:IPR039420|interpro:IPR036388	go:"GO:0000160"(phosphorelay signal transduction system)|go:"GO:0003677"(DNA binding)|go:"GO:0006355"(regulation of transcription, DNA-templated)|mint:J7M6Z1|refseq:WP_002991052.1|interpro:IPR011006(CheY-like)|interpro:IPR001867(Signal transduction response regulator, C-terminal)|interpro:IPR016032(Signal transduction response regulator, C-terminal effector)|interpro:IPR001789(Signal transduction response regulator, receiver region)|interpro:IPR039420|interpro:IPR036388	-	-	-	figure legend:fig. 1 a,b|comment:"Phosphorylation of recombinant CovR by the small molecule  phosphodonor acetyl phosphate induces homodimerization, which  results in slower migration on a native polyacrylamide gel  (Figure 1A).  An alanine substitution at D53 abrogates  phosphorylation at the altered site [22] and thereby homodimerization (Figure 1A)."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2020/11/03	2020/11/12	rogid:A/wz1/MIf63xiZHWOiEEBMfmaMI1207470	rogid:A/wz1/MIf63xiZHWOiEEBMfmaMI1207470	rigid:eBVEsZZ1+tPwjqJtuJvz770xRmA	false	modified L-aspartic acid residue:53-53|mutation disrupting interaction:53-53	modified L-aspartic acid residue:53-53|mutation disrupting interaction:53-53	2	0	psi-mi:"MI:0818"(molecular weight estimation by coomasie staining)	psi-mi:"MI:0818"(molecular weight estimation by coomasie staining)
uniprotkb:J7M6Z1	uniprotkb:A0A0H2UVV3	intact:EBI-26442404	intact:EBI-26442434|ensemblbacteria:AAM79976	psi-mi:j7m6z1_strp1(display_long)|uniprotkb:covR(gene name)|psi-mi:covR(display_short)|uniprotkb:M1GAS476_0346(orf name)	psi-mi:a0a0h2uvv3_strp3(display_long)|uniprotkb:SpyM3_1369(locus name)	psi-mi:"MI:0424"(protein kinase assay)	Horstmann et al. (2014)	imex:IM-28477|pubmed:24788524	taxid:1207470(Streptococcus pyogenes M1 476)|taxid:1207470(Streptococcus pyogenes M1 476)	taxid:198466("Streptococcus pyogenes serotype M3 (strain ATCC BAA-595 / MGAS315)")|taxid:198466("Streptococcus pyogenes serotype M3 (strain ATCC BAA-595 / MGAS315)")	psi-mi:"MI:0217"(phosphorylation reaction)	psi-mi:"MI:0471"(MINT)	intact:EBI-26442431|imex:IM-28477-3	-	-	psi-mi:"MI:0502"(enzyme target)	psi-mi:"MI:0501"(enzyme)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	go:"GO:0000160"(phosphorelay signal transduction system)|go:"GO:0003677"(DNA binding)|go:"GO:0006355"(regulation of transcription, DNA-templated)|mint:J7M6Z1|refseq:WP_002991052.1|interpro:IPR011006(CheY-like)|interpro:IPR001867(Signal transduction response regulator, C-terminal)|interpro:IPR016032(Signal transduction response regulator, C-terminal effector)|interpro:IPR001789(Signal transduction response regulator, receiver region)|interpro:IPR039420|interpro:IPR036388	ensemblbacteria:AAM79976(transcript)|ensemblbacteria:SpyM3_1369(gene)|go:"GO:0005524"(ATP binding)|go:"GO:0005886"(plasma membrane)|go:"GO:0007049"(cell cycle)|go:"GO:0008360"(regulation of cell shape)|go:"GO:0016021"(integral component of membrane)|go:"GO:0051301"(cell division)|go:"GO:0106310"(protein serine kinase activity)|go:"GO:0106311"|mint:A0A0H2UVV3|refseq:WP_002994648.1|interpro:IPR011009(Protein kinase-like)|interpro:IPR005543(PASTA)|interpro:IPR000719(Protein kinase, core)|interpro:IPR017441(Protein kinase ATP binding, conserved site)|interpro:IPR008271(Serine/threonine protein kinase, active site)	-	-	-	figure legend:fig. 2|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2020/11/03	2020/11/04	rogid:A/wz1/MIf63xiZHWOiEEBMfmaMI1207470	rogid:07+cCSUhCimhXMwtX1vaJKljvpM198466	rigid:mb1WGHyRrXPiDGDSlKnpTX+DkpM	false	O-phospho-L-threonine:65-65	binding-associated region:1-315	-	-	psi-mi:"MI:0427"(Identification by mass spectrometry)	psi-mi:"MI:0427"(Identification by mass spectrometry)
uniprotkb:J7M6Z1	uniprotkb:A0A0H2UVV3	intact:EBI-26442404	intact:EBI-26442434|ensemblbacteria:AAM79976	psi-mi:j7m6z1_strp1(display_long)|uniprotkb:covR(gene name)|psi-mi:covR(display_short)|uniprotkb:M1GAS476_0346(orf name)	psi-mi:a0a0h2uvv3_strp3(display_long)|uniprotkb:SpyM3_1369(locus name)	psi-mi:"MI:0424"(protein kinase assay)	Horstmann et al. (2014)	imex:IM-28477|pubmed:24788524	taxid:1207470(Streptococcus pyogenes M1 476)|taxid:1207470(Streptococcus pyogenes M1 476)	taxid:198466("Streptococcus pyogenes serotype M3 (strain ATCC BAA-595 / MGAS315)")|taxid:198466("Streptococcus pyogenes serotype M3 (strain ATCC BAA-595 / MGAS315)")	psi-mi:"MI:0217"(phosphorylation reaction)	psi-mi:"MI:0471"(MINT)	intact:EBI-26442606|imex:IM-28477-5	-	-	psi-mi:"MI:0502"(enzyme target)	psi-mi:"MI:0501"(enzyme)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	go:"GO:0000160"(phosphorelay signal transduction system)|go:"GO:0003677"(DNA binding)|go:"GO:0006355"(regulation of transcription, DNA-templated)|mint:J7M6Z1|refseq:WP_002991052.1|interpro:IPR011006(CheY-like)|interpro:IPR001867(Signal transduction response regulator, C-terminal)|interpro:IPR016032(Signal transduction response regulator, C-terminal effector)|interpro:IPR001789(Signal transduction response regulator, receiver region)|interpro:IPR039420|interpro:IPR036388	ensemblbacteria:AAM79976(transcript)|ensemblbacteria:SpyM3_1369(gene)|go:"GO:0005524"(ATP binding)|go:"GO:0005886"(plasma membrane)|go:"GO:0007049"(cell cycle)|go:"GO:0008360"(regulation of cell shape)|go:"GO:0016021"(integral component of membrane)|go:"GO:0051301"(cell division)|go:"GO:0106310"(protein serine kinase activity)|go:"GO:0106311"|mint:A0A0H2UVV3|refseq:WP_002994648.1|interpro:IPR011009(Protein kinase-like)|interpro:IPR005543(PASTA)|interpro:IPR000719(Protein kinase, core)|interpro:IPR017441(Protein kinase ATP binding, conserved site)|interpro:IPR008271(Serine/threonine protein kinase, active site)	-	-	-	figure legend:fig. 3|comment:"Consistent with our mass spectrometry data, incubation of CovR-T65A with StkKD resulted in no detectable phosphorylation using the Phos-tag assay (Figure 3A). In contrast, StkKD was able to phosphorylate CovR with an alanine substituted for threonine at position 73 (CovR-T73A, data not shown), further supporting the specific role of T65 in Stk-dependent CovR phosphorylation."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2020/11/03	2020/11/05	rogid:A/wz1/MIf63xiZHWOiEEBMfmaMI1207470	rogid:07+cCSUhCimhXMwtX1vaJKljvpM198466	rigid:mb1WGHyRrXPiDGDSlKnpTX+DkpM	false	mutation disrupting interaction:65-65|mutation disrupting interaction:65-65|mutation increasing interaction:53-53	binding-associated region:1-315	-	-	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)