#ID(s) interactor A	ID(s) interactor B	Alt. ID(s) interactor A	Alt. ID(s) interactor B	Alias(es) interactor A	Alias(es) interactor B	Interaction detection method(s)	Publication 1st author(s)	Publication Identifier(s)	Taxid interactor A	Taxid interactor B	Interaction type(s)	Source database(s)	Interaction identifier(s)	Confidence value(s)	Expansion method(s)	Biological role(s) interactor A	Biological role(s) interactor B	Experimental role(s) interactor A	Experimental role(s) interactor B	Type(s) interactor A	Type(s) interactor B	Xref(s) interactor A	Xref(s) interactor B	Interaction Xref(s)	Annotation(s) interactor A	Annotation(s) interactor B	Interaction annotation(s)	Host organism(s)	Interaction parameter(s)	Creation date	Update date	Checksum(s) interactor A	Checksum(s) interactor B	Interaction Checksum(s)	Negative	Feature(s) interactor A	Feature(s) interactor B	Stoichiometry(s) interactor A	Stoichiometry(s) interactor B	Identification method participant A	Identification method participant B
uniprotkb:P62837	uniprotkb:Q8X509	intact:EBI-347677|uniprotkb:P51669|uniprotkb:Q3MN78|uniprotkb:D3DQC9|uniprotkb:Q96RP6|ensembl:ENSP00000381717|ensembl:ENSP00000381718	intact:EBI-25438289|ensemblbacteria:BAB35579|ensemblbacteria:QCH82890|uniprotkb:A0A0H3JH14|uniprotkb:Q7ADX4|uniprotkb:A0A6M0JRY3	psi-mi:ub2d2_human(display_long)|uniprotkb:UBC5B(gene name synonym)|uniprotkb:UBCH4(gene name synonym)|uniprotkb:UBE2D2(gene name)|psi-mi:UBE2D2(display_short)|uniprotkb:UBC4(gene name synonym)|uniprotkb:UBCH5B(gene name synonym)|uniprotkb:Ubiquitin-protein ligase D2(gene name synonym)|uniprotkb:Ubiquitin carrier protein D2(gene name synonym)|uniprotkb:Ubiquitin-conjugating enzyme E2-17 kDa 2(gene name synonym)|uniprotkb:"Ubiquitin-conjugating enzyme E2(17)KB 2"(gene name synonym)|uniprotkb:PUBC1(gene name synonym)|uniprotkb:p53-regulated ubiquitin-conjugating enzyme 1(gene name synonym)|uniprotkb:"(E3-independent) E2 ubiquitin-conjugating enzyme D2"(gene name synonym)|uniprotkb:E2 ubiquitin-conjugating enzyme D2(gene name synonym)	psi-mi:q8x509_eco57(display_long)|uniprotkb:nleG2-3(gene name)|psi-mi:nleG2-3(display_short)|uniprotkb:ECs_2156(orf name)|uniprotkb:E5F08_13145(orf name)	psi-mi:"MI:0077"(nuclear magnetic resonance)	Wu et al. (2010)	pubmed:20585566|imex:IM-27765	taxid:9606(human)|taxid:9606(Homo sapiens)	taxid:83334(eco57)|taxid:83334("Escherichia coli O157:H7")	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0471"(MINT)	intact:EBI-25438287|imex:IM-27765-1	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_003330.1|refseq:NP_862821.1|dip:DIP-29267N|ensembl:ENSG00000131508(gene)|ensembl:ENST00000398733(transcript)|ensembl:ENST00000398734(transcript)|go:"GO:0000151"(ubiquitin ligase complex)|go:"GO:0000209"(protein polyubiquitination)|go:"GO:0004842"(ubiquitin-protein transferase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005634"(nucleus)|go:"GO:0005654"(nucleoplasm)|go:"GO:0005829"(cytosol)|go:"GO:0006464"(cellular protein modification process)|go:"GO:0006511"(ubiquitin-dependent protein catabolic process)|go:"GO:0016567"(protein ubiquitination)|go:"GO:0032991"(protein-containing complex)|go:"GO:0051865"(protein autoubiquitination)|go:"GO:0061630"(ubiquitin protein ligase activity)|go:"GO:0061631"(ubiquitin conjugating enzyme activity)|go:"GO:0070062"(extracellular exosome)|go:"GO:0070936"(protein K48-linked ubiquitination)|interpro:IPR000608(Ubiquitin-conjugating enzyme, E2)|interpro:IPR016135(Ubiquitin-conjugating enzyme/RWD-like)|interpro:IPR023313|mint:P62837|rcsb pdb:1UR6|rcsb pdb:1W4U|rcsb pdb:2CLW|rcsb pdb:2ESK|rcsb pdb:2ESO|rcsb pdb:2ESP|rcsb pdb:2ESQ|rcsb pdb:3A33|rcsb pdb:3JVZ|rcsb pdb:3JW0|rcsb pdb:3L1Y|rcsb pdb:3TGD|rcsb pdb:3ZNI|rcsb pdb:4A49|rcsb pdb:4A4B|rcsb pdb:4A4C|rcsb pdb:4AUQ|rcsb pdb:4DDG|rcsb pdb:4DDI|rcsb pdb:4LDT|rcsb pdb:4V3K|rcsb pdb:4V3L|rcsb pdb:4WZ3|rcsb pdb:5D0K|rcsb pdb:5D0M|rcsb pdb:5D1K|rcsb pdb:5D1L|rcsb pdb:5D1M|rcsb pdb:5EDV|rcsb pdb:5MNJ|rcsb pdb:5ULF|rcsb pdb:5ULH|rcsb pdb:5ULK|rcsb pdb:5VZW|rcsb pdb:5ZBU|rcsb pdb:6HPR|rcsb pdb:6M2C|rcsb pdb:6SQO|rcsb pdb:6SQR|rcsb pdb:6SQS|rcsb pdb:6TTU|rcsb pdb:6W7Z|rcsb pdb:6W9D|rcsb pdb:7AI0|rcsb pdb:7AI1|rcsb pdb:7BOL|reactome:R-HSA-1234176|reactome:R-HSA-168927|reactome:R-HSA-202424|reactome:R-HSA-2871837|reactome:R-HSA-5607764|reactome:R-HSA-8866652|reactome:R-HSA-8866654|reactome:R-HSA-8951664|reactome:R-HSA-9033241|reactome:R-HSA-936440|reactome:R-HSA-937041|reactome:R-HSA-9705462|reactome:R-HSA-983168	ensemblbacteria:BAB35579(transcript)|ensemblbacteria:CAM50_0014160(gene)|ensemblbacteria:ECs_2156(gene)|ensemblbacteria:QCH82890(transcript)|go:"GO:0004842"(ubiquitin-protein transferase activity)|go:"GO:0044403"(biological process involved in symbiotic interaction)|refseq:NP_310183.1|refseq:WP_001131658.1|rcsb pdb:2KKX|rcsb pdb:2KKY|interpro:IPR010489(Protein of unknown function DUF1076)|interpro:IPR038436	-	-	-	figure legend:fig. 6a-b|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2020/02/24	2020/03/02	rogid:vVGrEi0osU/WG0Y9qRCFMT4lgvg9606	rogid:tSug3VBBQ0q88LIn9xPI2Q/K9iA83334	rigid:UBZMZc79ATq3EK/vweoxpxitdqs	false	-	binding-associated region:90-191	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
uniprotkb:Q8X4X3	uniprotkb:P62837	intact:EBI-25438357|ensemblbacteria:BAB35419|ensemblbacteria:QCH83043|uniprotkb:A0A0H3JEU0|uniprotkb:Q7AE95	intact:EBI-347677|uniprotkb:P51669|uniprotkb:Q3MN78|uniprotkb:D3DQC9|uniprotkb:Q96RP6|ensembl:ENSP00000381717|ensembl:ENSP00000381718	psi-mi:q8x4x3_eco57(display_long)|uniprotkb:nleG5-1(gene name)|psi-mi:nleG5-1(display_short)|uniprotkb:ECs_1996(orf name)|uniprotkb:E5F08_13970(orf name)|uniprotkb:E5F08_18645(orf name)	psi-mi:ub2d2_human(display_long)|uniprotkb:UBC5B(gene name synonym)|uniprotkb:UBCH4(gene name synonym)|uniprotkb:UBE2D2(gene name)|psi-mi:UBE2D2(display_short)|uniprotkb:UBC4(gene name synonym)|uniprotkb:UBCH5B(gene name synonym)|uniprotkb:Ubiquitin-protein ligase D2(gene name synonym)|uniprotkb:Ubiquitin carrier protein D2(gene name synonym)|uniprotkb:Ubiquitin-conjugating enzyme E2-17 kDa 2(gene name synonym)|uniprotkb:"Ubiquitin-conjugating enzyme E2(17)KB 2"(gene name synonym)|uniprotkb:PUBC1(gene name synonym)|uniprotkb:p53-regulated ubiquitin-conjugating enzyme 1(gene name synonym)|uniprotkb:"(E3-independent) E2 ubiquitin-conjugating enzyme D2"(gene name synonym)|uniprotkb:E2 ubiquitin-conjugating enzyme D2(gene name synonym)	psi-mi:"MI:0053"(fluorescence polarization spectroscopy)	Wu et al. (2010)	pubmed:20585566|imex:IM-27765	taxid:83334(eco57)|taxid:83334("Escherichia coli O157:H7")	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0471"(MINT)	intact:EBI-25438319|imex:IM-27765-3	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	ensemblbacteria:BAB35419(transcript)|ensemblbacteria:CAM50_0015035(gene)|ensemblbacteria:ECs_1996(gene)|ensemblbacteria:QCH83043(transcript)|go:"GO:0004842"(ubiquitin-protein transferase activity)|go:"GO:0044403"(biological process involved in symbiotic interaction)|refseq:NP_310023.1|refseq:WP_001143784.1|rcsb pdb:6B3N|interpro:IPR010489(Protein of unknown function DUF1076)|interpro:IPR038436	refseq:NP_003330.1|refseq:NP_862821.1|dip:DIP-29267N|ensembl:ENSG00000131508(gene)|ensembl:ENST00000398733(transcript)|ensembl:ENST00000398734(transcript)|go:"GO:0000151"(ubiquitin ligase complex)|go:"GO:0000209"(protein polyubiquitination)|go:"GO:0004842"(ubiquitin-protein transferase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005634"(nucleus)|go:"GO:0005654"(nucleoplasm)|go:"GO:0005829"(cytosol)|go:"GO:0006464"(cellular protein modification process)|go:"GO:0006511"(ubiquitin-dependent protein catabolic process)|go:"GO:0016567"(protein ubiquitination)|go:"GO:0032991"(protein-containing complex)|go:"GO:0051865"(protein autoubiquitination)|go:"GO:0061630"(ubiquitin protein ligase activity)|go:"GO:0061631"(ubiquitin conjugating enzyme activity)|go:"GO:0070062"(extracellular exosome)|go:"GO:0070936"(protein K48-linked ubiquitination)|interpro:IPR000608(Ubiquitin-conjugating enzyme, E2)|interpro:IPR016135(Ubiquitin-conjugating enzyme/RWD-like)|interpro:IPR023313|mint:P62837|rcsb pdb:1UR6|rcsb pdb:1W4U|rcsb pdb:2CLW|rcsb pdb:2ESK|rcsb pdb:2ESO|rcsb pdb:2ESP|rcsb pdb:2ESQ|rcsb pdb:3A33|rcsb pdb:3JVZ|rcsb pdb:3JW0|rcsb pdb:3L1Y|rcsb pdb:3TGD|rcsb pdb:3ZNI|rcsb pdb:4A49|rcsb pdb:4A4B|rcsb pdb:4A4C|rcsb pdb:4AUQ|rcsb pdb:4DDG|rcsb pdb:4DDI|rcsb pdb:4LDT|rcsb pdb:4V3K|rcsb pdb:4V3L|rcsb pdb:4WZ3|rcsb pdb:5D0K|rcsb pdb:5D0M|rcsb pdb:5D1K|rcsb pdb:5D1L|rcsb pdb:5D1M|rcsb pdb:5EDV|rcsb pdb:5MNJ|rcsb pdb:5ULF|rcsb pdb:5ULH|rcsb pdb:5ULK|rcsb pdb:5VZW|rcsb pdb:5ZBU|rcsb pdb:6HPR|rcsb pdb:6M2C|rcsb pdb:6SQO|rcsb pdb:6SQR|rcsb pdb:6SQS|rcsb pdb:6TTU|rcsb pdb:6W7Z|rcsb pdb:6W9D|rcsb pdb:7AI0|rcsb pdb:7AI1|rcsb pdb:7BOL|reactome:R-HSA-1234176|reactome:R-HSA-168927|reactome:R-HSA-202424|reactome:R-HSA-2871837|reactome:R-HSA-5607764|reactome:R-HSA-8866652|reactome:R-HSA-8866654|reactome:R-HSA-8951664|reactome:R-HSA-9033241|reactome:R-HSA-936440|reactome:R-HSA-937041|reactome:R-HSA-9705462|reactome:R-HSA-983168	-	-	-	figure legend:fig. 7c|comment:"To quantify the NleG5-1[1–213] interactions with UBE2D2 variants, we labelled the three UBE2D2 mutant proteins described above with fluorescein-5-maleimide and monitored the change in fluorescence polarization upon titration of NleG5-1[1–213] (Figure 7C). Kd values for NleG5-1[1–213] interactions with UBE2D2 R5A, F62A and K63A mutants were 326±35, 36±2 and 213±9 µM, respectively. Compared to the Kd for NleG5-1[1–213] interaction with wild type UBE2D2, presented above (56±2 µM) the affinity of R5A and K63A mutants were approximately 5 and 3 times lower, respectively, while the affinity of the F62A mutant was slightly higher."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	kd:56.0x10^-6 ~2.0(molar)	2020/02/24	2020/03/02	rogid:hY+PmA0nI/a+CZ3MdCp0eYm/5DM83334	rogid:vVGrEi0osU/WG0Y9qRCFMT4lgvg9606	rigid:pxeRUJB6nVRGIOCkUeAJh1O/FlU	false	-	mutation increasing interaction rate:62-62|fluorescein-5-maleimide label:?-?|mutation decreasing interaction rate:5-5|mutation decreasing interaction rate:63-63	-	-	psi-mi:"MI:0867"(tag visualisation by fluorescence)	psi-mi:"MI:0867"(tag visualisation by fluorescence)