#ID(s) interactor A	ID(s) interactor B	Alt. ID(s) interactor A	Alt. ID(s) interactor B	Alias(es) interactor A	Alias(es) interactor B	Interaction detection method(s)	Publication 1st author(s)	Publication Identifier(s)	Taxid interactor A	Taxid interactor B	Interaction type(s)	Source database(s)	Interaction identifier(s)	Confidence value(s)	Expansion method(s)	Biological role(s) interactor A	Biological role(s) interactor B	Experimental role(s) interactor A	Experimental role(s) interactor B	Type(s) interactor A	Type(s) interactor B	Xref(s) interactor A	Xref(s) interactor B	Interaction Xref(s)	Annotation(s) interactor A	Annotation(s) interactor B	Interaction annotation(s)	Host organism(s)	Interaction parameter(s)	Creation date	Update date	Checksum(s) interactor A	Checksum(s) interactor B	Interaction Checksum(s)	Negative	Feature(s) interactor A	Feature(s) interactor B	Stoichiometry(s) interactor A	Stoichiometry(s) interactor B	Identification method participant A	Identification method participant B
uniprotkb:Q8X482	uniprotkb:A0A287ATX4	intact:EBI-22229752|ensemblbacteria:AAG56991	intact:EBI-25339265|ensembl:ENSSSCP00000047552	psi-mi:espfu_eco57(display_long)|uniprotkb:EspF-like protein encoded on prophage U(gene name synonym)|uniprotkb:Tir-cytoskeleton coupling protein TccP(gene name synonym)|uniprotkb:"espF(U)"(gene name)|psi-mi:"espF(U)"(display_short)|uniprotkb:tccP(gene name synonym)|uniprotkb:Z3072(locus name)	psi-mi:a0a287atx4_pig(display_long)|uniprotkb:WASL(gene name)|psi-mi:WASL(display_short)	psi-mi:"MI:0096"(pull down)	Campellone et al. (2008)	pubmed:18974829|imex:IM-27627	taxid:83334(eco57)|taxid:83334("Escherichia coli O157:H7")	taxid:9823(pig)|taxid:9823("Sus scrofa (Pig)")	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0471"(MINT)	intact:EBI-25339252|imex:IM-27627-1	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	ensemblbacteria:AAG56991(transcript)|ensemblbacteria:Z3072(gene)|go:"GO:0005576"(extracellular region)|go:"GO:0030430"(host cell cytoplasm)|mint:Q8X482|interpro:IPR006891(EspF protein)	ensembl:ENSSSCG00000016604(gene)|ensembl:ENSSSCT00000043210(transcript)|go:"GO:0003779"(actin binding)|go:"GO:0005634"(nucleus)|go:"GO:0007015"(actin filament organization)|go:"GO:2000601"(positive regulation of Arp2/3 complex-mediated actin nucleation)|interpro:IPR000095(PAK-box/P21-Rho-binding)|interpro:IPR000697(EVH1)|interpro:IPR003124(Actin-binding WH2)|interpro:IPR011026(Wiscott-Aldrich syndrome, C-terminal)|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR030214|interpro:IPR033927|interpro:IPR036936|mint:A0A287ATX4|refseq:XP_003134773.1	-	-	-	figure legend:fig. 4b, 4c|comment:"EspFU-R1-6, the derivative containing  the entire C-terminus of EspFU, pulled down N-WASP, whereas the  EspFU-N control fragment did not (Figure 4B). The efficiency of NWASP pulldown appeared to be slightly lower for variants  containing fewer than four repeats, whereas mutants containing  only a single repeat bound N-WASP at substantially decreased levels  (R1-6 = R1-4.R1-3 = R1-2&R1 = R6)."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2020/01/07	2020/01/08	rogid:5ta4O6gj+U5E7doG3zmjLy36nJ483334	rogid:Nq2jAJqzjm3eSaqtAlnI0YVg36A9823	rigid:FCSG0VxFxrn+pJhOFTJq0mePOfA	false	his tag:n-n|myc tag:c-c|sufficient binding region:80-384	-	-	-	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)
uniprotkb:O08816	uniprotkb:Q8X482	intact:EBI-6142604	intact:EBI-22229752|ensemblbacteria:AAG56991	psi-mi:wasl_rat(display_long)|uniprotkb:Wasl(gene name)|psi-mi:Wasl(display_short)	psi-mi:espfu_eco57(display_long)|uniprotkb:EspF-like protein encoded on prophage U(gene name synonym)|uniprotkb:Tir-cytoskeleton coupling protein TccP(gene name synonym)|uniprotkb:"espF(U)"(gene name)|psi-mi:"espF(U)"(display_short)|uniprotkb:tccP(gene name synonym)|uniprotkb:Z3072(locus name)	psi-mi:"MI:0018"(two hybrid)	Campellone et al. (2008)	pubmed:18974829|imex:IM-27627	taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)")	taxid:83334(eco57)|taxid:83334("Escherichia coli O157:H7")	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0471"(MINT)	intact:EBI-25339660|imex:IM-27627-3	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	mint:O08816|rgd:735144|dip:DIP-17016N|go:"GO:0003779"(actin binding)|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005884"(actin filament)|go:"GO:0006900"(vesicle budding from membrane)|go:"GO:0008092"(cytoskeletal protein binding)|go:"GO:0009617"(response to bacterium)|go:"GO:0010324"(membrane invagination)|go:"GO:0016050"(vesicle organization)|go:"GO:0030027"(lamellipodium)|go:"GO:0030036"(actin cytoskeleton organization)|go:"GO:0030041"(actin filament polymerization)|go:"GO:0030048"(actin filament-based movement)|go:"GO:0030050"(vesicle transport along actin filament)|go:"GO:0030478"(actin cap)|go:"GO:0031252"(cell leading edge)|go:"GO:0031410"(cytoplasmic vesicle)|go:"GO:0032880"(regulation of protein localization)|go:"GO:0034629"|go:"GO:0042802"(identical protein binding)|go:"GO:0045944"(positive regulation of transcription by RNA polymerase II)|go:"GO:0050921"(positive regulation of chemotaxis)|go:"GO:0051301"(cell division)|go:"GO:0051491"(positive regulation of filopodium assembly)|go:"GO:0051653"(spindle localization)|go:"GO:0060491"(regulation of cell projection assembly)|go:"GO:0060997"(dendritic spine morphogenesis)|go:"GO:0097320"(plasma membrane tubulation)|go:"GO:0098794"(postsynapse)|go:"GO:0098974"(postsynaptic actin cytoskeleton organization)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:1903526"(negative regulation of membrane tubulation)|go:"GO:2000370"(positive regulation of clathrin-dependent endocytosis)|go:"GO:2000402"(negative regulation of lymphocyte migration)|go:"GO:2000601"(positive regulation of Arp2/3 complex-mediated actin nucleation)|interpro:IPR000095(PAK-box/P21-Rho-binding)|interpro:IPR000697(EVH1)|interpro:IPR003124(Actin-binding WH2)|interpro:IPR011026(Wiscott-Aldrich syndrome, C-terminal)|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR030214|interpro:IPR033927|interpro:IPR036936|rcsb pdb:1MKE|rcsb pdb:2IFS|go:"GO:0000139"(Golgi membrane)	ensemblbacteria:AAG56991(transcript)|ensemblbacteria:Z3072(gene)|go:"GO:0005576"(extracellular region)|go:"GO:0030430"(host cell cytoplasm)|mint:Q8X482|interpro:IPR006891(EspF protein)	-	-	-	figure legend:fig. 7d|comment:"we examined this interaction in yeast two-hybrid assays. As demonstrated previously [28], the N-WASP GBD interacted with the C-terminus of EspFU that contains the repeat sequences, but not the EspFU N-terminus (Figure 7D). In addition, all of the C-terminal subfragments of EspFU, even those containing only a single repeat, interacted with the GBD in these assays. However, the degree of interaction did not positively correlate with increasing numbers of repeats. Rather, the number of repeats in EspFU inversely correlated with the degree of expression of the lacZ reporter."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:4932(yeasx)|taxid:4932("Saccharomyces cerevisiae (Baker's yeast)")	-	2020/01/07	2020/01/09	rogid:lKgHtUQQEc8C14/X9D8WOvry2iM10116	rogid:5ta4O6gj+U5E7doG3zmjLy36nJ483334	rigid:kpjsi7O6TBAKQFHiSDdtZHwSXD0	false	binding-associated region:151-273	sufficient binding region:80-134|sufficient binding region:323-384|necessary binding region:80-384	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
uniprotkb:O08816	uniprotkb:Q8X482	intact:EBI-6142604	intact:EBI-22229752|ensemblbacteria:AAG56991	psi-mi:wasl_rat(display_long)|uniprotkb:Wasl(gene name)|psi-mi:Wasl(display_short)	psi-mi:espfu_eco57(display_long)|uniprotkb:EspF-like protein encoded on prophage U(gene name synonym)|uniprotkb:Tir-cytoskeleton coupling protein TccP(gene name synonym)|uniprotkb:"espF(U)"(gene name)|psi-mi:"espF(U)"(display_short)|uniprotkb:tccP(gene name synonym)|uniprotkb:Z3072(locus name)	psi-mi:"MI:0065"(isothermal titration calorimetry)	Campellone et al. (2008)	pubmed:18974829|imex:IM-27627	taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)")	taxid:83334(eco57)|taxid:83334("Escherichia coli O157:H7")	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0471"(MINT)	intact:EBI-25339679|imex:IM-27627-5	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	mint:O08816|rgd:735144|dip:DIP-17016N|go:"GO:0003779"(actin binding)|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005884"(actin filament)|go:"GO:0006900"(vesicle budding from membrane)|go:"GO:0008092"(cytoskeletal protein binding)|go:"GO:0009617"(response to bacterium)|go:"GO:0010324"(membrane invagination)|go:"GO:0016050"(vesicle organization)|go:"GO:0030027"(lamellipodium)|go:"GO:0030036"(actin cytoskeleton organization)|go:"GO:0030041"(actin filament polymerization)|go:"GO:0030048"(actin filament-based movement)|go:"GO:0030050"(vesicle transport along actin filament)|go:"GO:0030478"(actin cap)|go:"GO:0031252"(cell leading edge)|go:"GO:0031410"(cytoplasmic vesicle)|go:"GO:0032880"(regulation of protein localization)|go:"GO:0034629"|go:"GO:0042802"(identical protein binding)|go:"GO:0045944"(positive regulation of transcription by RNA polymerase II)|go:"GO:0050921"(positive regulation of chemotaxis)|go:"GO:0051301"(cell division)|go:"GO:0051491"(positive regulation of filopodium assembly)|go:"GO:0051653"(spindle localization)|go:"GO:0060491"(regulation of cell projection assembly)|go:"GO:0060997"(dendritic spine morphogenesis)|go:"GO:0097320"(plasma membrane tubulation)|go:"GO:0098794"(postsynapse)|go:"GO:0098974"(postsynaptic actin cytoskeleton organization)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:1903526"(negative regulation of membrane tubulation)|go:"GO:2000370"(positive regulation of clathrin-dependent endocytosis)|go:"GO:2000402"(negative regulation of lymphocyte migration)|go:"GO:2000601"(positive regulation of Arp2/3 complex-mediated actin nucleation)|interpro:IPR000095(PAK-box/P21-Rho-binding)|interpro:IPR000697(EVH1)|interpro:IPR003124(Actin-binding WH2)|interpro:IPR011026(Wiscott-Aldrich syndrome, C-terminal)|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR030214|interpro:IPR033927|interpro:IPR036936|rcsb pdb:1MKE|rcsb pdb:2IFS|go:"GO:0000139"(Golgi membrane)	ensemblbacteria:AAG56991(transcript)|ensemblbacteria:Z3072(gene)|go:"GO:0005576"(extracellular region)|go:"GO:0030430"(host cell cytoplasm)|mint:Q8X482|interpro:IPR006891(EspF protein)	-	-	-	figure legend:fig. 8a|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2020/01/07	2020/01/09	rogid:lKgHtUQQEc8C14/X9D8WOvry2iM10116	rogid:5ta4O6gj+U5E7doG3zmjLy36nJ483334	rigid:kpjsi7O6TBAKQFHiSDdtZHwSXD0	false	binding-associated region:151-273	binding-associated region:268-314|binding-associated region:221-314|binding-associated region:80-314	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
uniprotkb:O08816	uniprotkb:Q8X482	intact:EBI-6142604	intact:EBI-22229752|ensemblbacteria:AAG56991	psi-mi:wasl_rat(display_long)|uniprotkb:Wasl(gene name)|psi-mi:Wasl(display_short)	psi-mi:espfu_eco57(display_long)|uniprotkb:EspF-like protein encoded on prophage U(gene name synonym)|uniprotkb:Tir-cytoskeleton coupling protein TccP(gene name synonym)|uniprotkb:"espF(U)"(gene name)|psi-mi:"espF(U)"(display_short)|uniprotkb:tccP(gene name synonym)|uniprotkb:Z3072(locus name)	psi-mi:"MI:0071"(molecular sieving)	Campellone et al. (2008)	pubmed:18974829|imex:IM-27627	taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)")	taxid:83334(eco57)|taxid:83334("Escherichia coli O157:H7")	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-25339792|imex:IM-27627-7	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	mint:O08816|rgd:735144|dip:DIP-17016N|go:"GO:0003779"(actin binding)|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005884"(actin filament)|go:"GO:0006900"(vesicle budding from membrane)|go:"GO:0008092"(cytoskeletal protein binding)|go:"GO:0009617"(response to bacterium)|go:"GO:0010324"(membrane invagination)|go:"GO:0016050"(vesicle organization)|go:"GO:0030027"(lamellipodium)|go:"GO:0030036"(actin cytoskeleton organization)|go:"GO:0030041"(actin filament polymerization)|go:"GO:0030048"(actin filament-based movement)|go:"GO:0030050"(vesicle transport along actin filament)|go:"GO:0030478"(actin cap)|go:"GO:0031252"(cell leading edge)|go:"GO:0031410"(cytoplasmic vesicle)|go:"GO:0032880"(regulation of protein localization)|go:"GO:0034629"|go:"GO:0042802"(identical protein binding)|go:"GO:0045944"(positive regulation of transcription by RNA polymerase II)|go:"GO:0050921"(positive regulation of chemotaxis)|go:"GO:0051301"(cell division)|go:"GO:0051491"(positive regulation of filopodium assembly)|go:"GO:0051653"(spindle localization)|go:"GO:0060491"(regulation of cell projection assembly)|go:"GO:0060997"(dendritic spine morphogenesis)|go:"GO:0097320"(plasma membrane tubulation)|go:"GO:0098794"(postsynapse)|go:"GO:0098974"(postsynaptic actin cytoskeleton organization)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:1903526"(negative regulation of membrane tubulation)|go:"GO:2000370"(positive regulation of clathrin-dependent endocytosis)|go:"GO:2000402"(negative regulation of lymphocyte migration)|go:"GO:2000601"(positive regulation of Arp2/3 complex-mediated actin nucleation)|interpro:IPR000095(PAK-box/P21-Rho-binding)|interpro:IPR000697(EVH1)|interpro:IPR003124(Actin-binding WH2)|interpro:IPR011026(Wiscott-Aldrich syndrome, C-terminal)|interpro:IPR011993(Pleckstrin homology-type)|interpro:IPR030214|interpro:IPR033927|interpro:IPR036936|rcsb pdb:1MKE|rcsb pdb:2IFS|go:"GO:0000139"(Golgi membrane)	ensemblbacteria:AAG56991(transcript)|ensemblbacteria:Z3072(gene)|go:"GO:0005576"(extracellular region)|go:"GO:0030430"(host cell cytoplasm)|mint:Q8X482|interpro:IPR006891(EspF protein)	-	-	-	figure legend:fig. 8b|comment:"The single EspFU repeat bound N-WASP at essentially stoichiometric levels when mixed in equimolar (2 µM) quantities, as determined by an increase in the apparent size (i.e., earlier elution profile) of EspFU (Figure 8B left, red vs. blue peaks). The addition of 2 µM (i.e., a two-fold higher molar concentration) Arp2/3 caused ∼60% of the EspFU to shift to an even more rapidly eluting fraction indicative of an EspFU-N-WASP-Arp2/3 complex (Figure 8B, yellow profile). Very little of this complex was detected using 1 µM Arp2/3 (Figure 8B left, small shoulder in gray profile)."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2020/01/07	2020/01/09	rogid:lKgHtUQQEc8C14/X9D8WOvry2iM10116	rogid:5ta4O6gj+U5E7doG3zmjLy36nJ483334	rigid:kpjsi7O6TBAKQFHiSDdtZHwSXD0	false	binding-associated region:193-501	binding-associated region:268-314|binding-associated region:221-314	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)