#ID(s) interactor A	ID(s) interactor B	Alt. ID(s) interactor A	Alt. ID(s) interactor B	Alias(es) interactor A	Alias(es) interactor B	Interaction detection method(s)	Publication 1st author(s)	Publication Identifier(s)	Taxid interactor A	Taxid interactor B	Interaction type(s)	Source database(s)	Interaction identifier(s)	Confidence value(s)	Expansion method(s)	Biological role(s) interactor A	Biological role(s) interactor B	Experimental role(s) interactor A	Experimental role(s) interactor B	Type(s) interactor A	Type(s) interactor B	Xref(s) interactor A	Xref(s) interactor B	Interaction Xref(s)	Annotation(s) interactor A	Annotation(s) interactor B	Interaction annotation(s)	Host organism(s)	Interaction parameter(s)	Creation date	Update date	Checksum(s) interactor A	Checksum(s) interactor B	Interaction Checksum(s)	Negative	Feature(s) interactor A	Feature(s) interactor B	Stoichiometry(s) interactor A	Stoichiometry(s) interactor B	Identification method participant A	Identification method participant B
uniprotkb:P25656	uniprotkb:P39524	intact:EBI-22014|uniprotkb:D6VR94|ensemblfungi:YCR094W	intact:EBI-3106|uniprotkb:D6VPJ2|ensemblfungi:YAL026C	psi-mi:cdc50_yeast(display_long)|uniprotkb:YCR94W(orf name)|uniprotkb:YCR094W(locus name)|uniprotkb:CDC50(gene name)|psi-mi:CDC50(display_short)	psi-mi:atc3_yeast(display_long)|uniprotkb:DRS2(gene name)|psi-mi:DRS2(display_short)|uniprotkb:FUN38(orf name)|uniprotkb:YAL026C(locus name)	psi-mi:"MI:0410"(3D electron microscopy)	Timcenko et al. (2019)	pubmed:31243363|imex:IM-28392	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0465"(DIP)	intact:EBI-21496101|wwpdb:6ROH|wwpdb:6ROI|emdb:EMD-4972|emdb:EMD-4973|imex:IM-28392-1	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_010018.1|sgd:S000000690|dip:DIP-5138N|ensemblfungi:YCR094W(gene)|ensemblfungi:YCR094W_mRNA(transcript)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005802"(trans-Golgi network)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006897"(endocytosis)|go:"GO:0016021"(integral component of membrane)|go:"GO:0031902"(late endosome membrane)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:0045332"(phospholipid translocation)|go:"GO:0051666"(actin cortical patch localization)|go:"GO:0140331"(aminophospholipid translocation)|go:"GO:1990530"(Cdc50p-Drs2p complex)|interpro:IPR005045(Protein of unknown function DUF284, transmembrane eukaryotic)|rcsb pdb:6PSY|rcsb pdb:6ROH|rcsb pdb:6ROI|rcsb pdb:6ROJ|rcsb pdb:7OH4|rcsb pdb:7OH6|rcsb pdb:7OH7|reactome:R-SCE-6798695|rcsb pdb:6PSX|mint:P25656	refseq:NP_009376.2|sgd:S000000024|dip:DIP-2216N|mint:P39524|ensemblfungi:YAL026C(gene)|ensemblfungi:YAL026C_mRNA(transcript)|go:"GO:0000287"(magnesium ion binding)|go:"GO:0000749"(response to pheromone triggering conjugation with cellular fusion)|go:"GO:0005524"(ATP binding)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005802"(trans-Golgi network)|go:"GO:0005886"(plasma membrane)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006892"(post-Golgi vesicle-mediated transport)|go:"GO:0006897"(endocytosis)|go:"GO:0016021"(integral component of membrane)|go:"GO:0032456"(endocytic recycling)|go:"GO:0045332"(phospholipid translocation)|go:"GO:0090555"(phosphatidylethanolamine flippase activity)|go:"GO:0140326"(ATPase-coupled intramembrane lipid transporter activity)|go:"GO:0140345"(phosphatidylcholine flippase activity)|go:"GO:0140346"(phosphatidylserine flippase activity)|go:"GO:1990530"(Cdc50p-Drs2p complex)|interpro:IPR001757(ATPase, P-type, K/Mg/Cd/Cu/Zn/Na/Ca/Na/H-transporter)|interpro:IPR006539(Phospholipid-translocating P-type ATPase, flippase)|interpro:IPR008250(E1-E2 ATPase-associated region)|interpro:IPR018303|interpro:IPR023214|interpro:IPR023298|interpro:IPR023299|interpro:IPR032630|interpro:IPR032631|interpro:IPR036412|interpro:IPR044492|rcsb pdb:6PSX|rcsb pdb:6PSY|rcsb pdb:6ROH|rcsb pdb:6ROI|rcsb pdb:6ROJ|rcsb pdb:7OH4|rcsb pdb:7OH5|rcsb pdb:7OH6|rcsb pdb:7OH7|reactome:R-SCE-6798695|reactome:R-SCE-936837	-	crc64:565575CA402C0C91	crc64:5745B92901F8E1AE	figure legend:Ext Data Tab 1|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	-	2019/07/08	2019/07/08	rogid:zVi09MNh1hpBUuc5AzasxmCrnKY559292	rogid:qJ8uK7dznIqyjVwjbw/VGn5Bo+4559292	rigid:H/6/aNvfGyK1lAEOYrg5Yd83mgw	false	-	binding-associated region:105-1355	1	1	psi-mi:"MI:2287"(identification by structure determination)	psi-mi:"MI:2287"(identification by structure determination)
uniprotkb:P25656	uniprotkb:P39524	intact:EBI-22014|uniprotkb:D6VR94|ensemblfungi:YCR094W	intact:EBI-3106|uniprotkb:D6VPJ2|ensemblfungi:YAL026C	psi-mi:cdc50_yeast(display_long)|uniprotkb:YCR94W(orf name)|uniprotkb:YCR094W(locus name)|uniprotkb:CDC50(gene name)|psi-mi:CDC50(display_short)	psi-mi:atc3_yeast(display_long)|uniprotkb:DRS2(gene name)|psi-mi:DRS2(display_short)|uniprotkb:FUN38(orf name)|uniprotkb:YAL026C(locus name)	psi-mi:"MI:0410"(3D electron microscopy)	Timcenko et al. (2019)	pubmed:31243363|imex:IM-28392	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0465"(DIP)	intact:EBI-21496111|wwpdb:6ROJ|emdb:EMD-4974|imex:IM-28392-3	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_010018.1|sgd:S000000690|dip:DIP-5138N|ensemblfungi:YCR094W(gene)|ensemblfungi:YCR094W_mRNA(transcript)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005802"(trans-Golgi network)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006897"(endocytosis)|go:"GO:0016021"(integral component of membrane)|go:"GO:0031902"(late endosome membrane)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:0045332"(phospholipid translocation)|go:"GO:0051666"(actin cortical patch localization)|go:"GO:0140331"(aminophospholipid translocation)|go:"GO:1990530"(Cdc50p-Drs2p complex)|interpro:IPR005045(Protein of unknown function DUF284, transmembrane eukaryotic)|rcsb pdb:6PSY|rcsb pdb:6ROH|rcsb pdb:6ROI|rcsb pdb:6ROJ|rcsb pdb:7OH4|rcsb pdb:7OH6|rcsb pdb:7OH7|reactome:R-SCE-6798695|rcsb pdb:6PSX|mint:P25656	refseq:NP_009376.2|sgd:S000000024|dip:DIP-2216N|mint:P39524|ensemblfungi:YAL026C(gene)|ensemblfungi:YAL026C_mRNA(transcript)|go:"GO:0000287"(magnesium ion binding)|go:"GO:0000749"(response to pheromone triggering conjugation with cellular fusion)|go:"GO:0005524"(ATP binding)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005802"(trans-Golgi network)|go:"GO:0005886"(plasma membrane)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006892"(post-Golgi vesicle-mediated transport)|go:"GO:0006897"(endocytosis)|go:"GO:0016021"(integral component of membrane)|go:"GO:0032456"(endocytic recycling)|go:"GO:0045332"(phospholipid translocation)|go:"GO:0090555"(phosphatidylethanolamine flippase activity)|go:"GO:0140326"(ATPase-coupled intramembrane lipid transporter activity)|go:"GO:0140345"(phosphatidylcholine flippase activity)|go:"GO:0140346"(phosphatidylserine flippase activity)|go:"GO:1990530"(Cdc50p-Drs2p complex)|interpro:IPR001757(ATPase, P-type, K/Mg/Cd/Cu/Zn/Na/Ca/Na/H-transporter)|interpro:IPR006539(Phospholipid-translocating P-type ATPase, flippase)|interpro:IPR008250(E1-E2 ATPase-associated region)|interpro:IPR018303|interpro:IPR023214|interpro:IPR023298|interpro:IPR023299|interpro:IPR032630|interpro:IPR032631|interpro:IPR036412|interpro:IPR044492|rcsb pdb:6PSX|rcsb pdb:6PSY|rcsb pdb:6ROH|rcsb pdb:6ROI|rcsb pdb:6ROJ|rcsb pdb:7OH4|rcsb pdb:7OH5|rcsb pdb:7OH6|rcsb pdb:7OH7|reactome:R-SCE-6798695|reactome:R-SCE-936837	-	crc64:565575CA402C0C91	crc64:5745B92901F8E1AE	full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	-	2019/07/08	2019/07/08	rogid:zVi09MNh1hpBUuc5AzasxmCrnKY559292	rogid:qJ8uK7dznIqyjVwjbw/VGn5Bo+4559292	rigid:H/6/aNvfGyK1lAEOYrg5Yd83mgw	false	-	binding-associated region:105-1247	1	1	psi-mi:"MI:2287"(identification by structure determination)	psi-mi:"MI:2287"(identification by structure determination)
uniprotkb:P25656	uniprotkb:P39524	intact:EBI-22014|uniprotkb:D6VR94|ensemblfungi:YCR094W	intact:EBI-3106|uniprotkb:D6VPJ2|ensemblfungi:YAL026C	psi-mi:cdc50_yeast(display_long)|uniprotkb:YCR94W(orf name)|uniprotkb:YCR094W(locus name)|uniprotkb:CDC50(gene name)|psi-mi:CDC50(display_short)	psi-mi:atc3_yeast(display_long)|uniprotkb:DRS2(gene name)|psi-mi:DRS2(display_short)|uniprotkb:FUN38(orf name)|uniprotkb:YAL026C(locus name)	psi-mi:"MI:0071"(molecular sieving)	Timcenko et al. (2019)	pubmed:31243363|imex:IM-28392	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0465"(DIP)	intact:EBI-21496126|imex:IM-28392-5	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_010018.1|sgd:S000000690|dip:DIP-5138N|ensemblfungi:YCR094W(gene)|ensemblfungi:YCR094W_mRNA(transcript)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005802"(trans-Golgi network)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006897"(endocytosis)|go:"GO:0016021"(integral component of membrane)|go:"GO:0031902"(late endosome membrane)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:0045332"(phospholipid translocation)|go:"GO:0051666"(actin cortical patch localization)|go:"GO:0140331"(aminophospholipid translocation)|go:"GO:1990530"(Cdc50p-Drs2p complex)|interpro:IPR005045(Protein of unknown function DUF284, transmembrane eukaryotic)|rcsb pdb:6PSY|rcsb pdb:6ROH|rcsb pdb:6ROI|rcsb pdb:6ROJ|rcsb pdb:7OH4|rcsb pdb:7OH6|rcsb pdb:7OH7|reactome:R-SCE-6798695|rcsb pdb:6PSX|mint:P25656	refseq:NP_009376.2|sgd:S000000024|dip:DIP-2216N|mint:P39524|ensemblfungi:YAL026C(gene)|ensemblfungi:YAL026C_mRNA(transcript)|go:"GO:0000287"(magnesium ion binding)|go:"GO:0000749"(response to pheromone triggering conjugation with cellular fusion)|go:"GO:0005524"(ATP binding)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005802"(trans-Golgi network)|go:"GO:0005886"(plasma membrane)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006892"(post-Golgi vesicle-mediated transport)|go:"GO:0006897"(endocytosis)|go:"GO:0016021"(integral component of membrane)|go:"GO:0032456"(endocytic recycling)|go:"GO:0045332"(phospholipid translocation)|go:"GO:0090555"(phosphatidylethanolamine flippase activity)|go:"GO:0140326"(ATPase-coupled intramembrane lipid transporter activity)|go:"GO:0140345"(phosphatidylcholine flippase activity)|go:"GO:0140346"(phosphatidylserine flippase activity)|go:"GO:1990530"(Cdc50p-Drs2p complex)|interpro:IPR001757(ATPase, P-type, K/Mg/Cd/Cu/Zn/Na/Ca/Na/H-transporter)|interpro:IPR006539(Phospholipid-translocating P-type ATPase, flippase)|interpro:IPR008250(E1-E2 ATPase-associated region)|interpro:IPR018303|interpro:IPR023214|interpro:IPR023298|interpro:IPR023299|interpro:IPR032630|interpro:IPR032631|interpro:IPR036412|interpro:IPR044492|rcsb pdb:6PSX|rcsb pdb:6PSY|rcsb pdb:6ROH|rcsb pdb:6ROI|rcsb pdb:6ROJ|rcsb pdb:7OH4|rcsb pdb:7OH5|rcsb pdb:7OH6|rcsb pdb:7OH7|reactome:R-SCE-6798695|reactome:R-SCE-936837	-	crc64:565575CA402C0C91	crc64:5745B92901F8E1AE	figure legend:Ext Data Fig 2a|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	-	2019/07/08	2019/07/08	rogid:zVi09MNh1hpBUuc5AzasxmCrnKY559292	rogid:qJ8uK7dznIqyjVwjbw/VGn5Bo+4559292	rigid:H/6/aNvfGyK1lAEOYrg5Yd83mgw	false	-	sufficient binding region:105-1247	-	-	psi-mi:"MI:2287"(identification by structure determination)	psi-mi:"MI:2287"(identification by structure determination)
uniprotkb:P39524	uniprotkb:P25656	intact:EBI-3106|uniprotkb:D6VPJ2|ensemblfungi:YAL026C	intact:EBI-22014|uniprotkb:D6VR94|ensemblfungi:YCR094W	psi-mi:atc3_yeast(display_long)|uniprotkb:DRS2(gene name)|psi-mi:DRS2(display_short)|uniprotkb:FUN38(orf name)|uniprotkb:YAL026C(locus name)	psi-mi:cdc50_yeast(display_long)|uniprotkb:YCR94W(orf name)|uniprotkb:YCR094W(locus name)|uniprotkb:CDC50(gene name)|psi-mi:CDC50(display_short)	psi-mi:"MI:0096"(pull down)	Timcenko et al. (2019)	pubmed:31243363|imex:IM-28392	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0465"(DIP)	intact:EBI-21496145|imex:IM-28392-7	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_009376.2|sgd:S000000024|dip:DIP-2216N|mint:P39524|ensemblfungi:YAL026C(gene)|ensemblfungi:YAL026C_mRNA(transcript)|go:"GO:0000287"(magnesium ion binding)|go:"GO:0000749"(response to pheromone triggering conjugation with cellular fusion)|go:"GO:0005524"(ATP binding)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005802"(trans-Golgi network)|go:"GO:0005886"(plasma membrane)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006892"(post-Golgi vesicle-mediated transport)|go:"GO:0006897"(endocytosis)|go:"GO:0016021"(integral component of membrane)|go:"GO:0032456"(endocytic recycling)|go:"GO:0045332"(phospholipid translocation)|go:"GO:0090555"(phosphatidylethanolamine flippase activity)|go:"GO:0140326"(ATPase-coupled intramembrane lipid transporter activity)|go:"GO:0140345"(phosphatidylcholine flippase activity)|go:"GO:0140346"(phosphatidylserine flippase activity)|go:"GO:1990530"(Cdc50p-Drs2p complex)|interpro:IPR001757(ATPase, P-type, K/Mg/Cd/Cu/Zn/Na/Ca/Na/H-transporter)|interpro:IPR006539(Phospholipid-translocating P-type ATPase, flippase)|interpro:IPR008250(E1-E2 ATPase-associated region)|interpro:IPR018303|interpro:IPR023214|interpro:IPR023298|interpro:IPR023299|interpro:IPR032630|interpro:IPR032631|interpro:IPR036412|interpro:IPR044492|rcsb pdb:6PSX|rcsb pdb:6PSY|rcsb pdb:6ROH|rcsb pdb:6ROI|rcsb pdb:6ROJ|rcsb pdb:7OH4|rcsb pdb:7OH5|rcsb pdb:7OH6|rcsb pdb:7OH7|reactome:R-SCE-6798695|reactome:R-SCE-936837	refseq:NP_010018.1|sgd:S000000690|dip:DIP-5138N|ensemblfungi:YCR094W(gene)|ensemblfungi:YCR094W_mRNA(transcript)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005802"(trans-Golgi network)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006897"(endocytosis)|go:"GO:0016021"(integral component of membrane)|go:"GO:0031902"(late endosome membrane)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:0045332"(phospholipid translocation)|go:"GO:0051666"(actin cortical patch localization)|go:"GO:0140331"(aminophospholipid translocation)|go:"GO:1990530"(Cdc50p-Drs2p complex)|interpro:IPR005045(Protein of unknown function DUF284, transmembrane eukaryotic)|rcsb pdb:6PSY|rcsb pdb:6ROH|rcsb pdb:6ROI|rcsb pdb:6ROJ|rcsb pdb:7OH4|rcsb pdb:7OH6|rcsb pdb:7OH7|reactome:R-SCE-6798695|rcsb pdb:6PSX|mint:P25656	-	crc64:5745B92901F8E1AE	crc64:565575CA402C0C91	figure legend:Ext Data Fig 4a|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	-	2019/07/08	2019/07/08	rogid:qJ8uK7dznIqyjVwjbw/VGn5Bo+4559292	rogid:zVi09MNh1hpBUuc5AzasxmCrnKY559292	rigid:H/6/aNvfGyK1lAEOYrg5Yd83mgw	false	biotin tag:c-c|mutation with no effect:1235-1235|mutation with no effect:1235-1235|mutation with no effect:1236-1236	-	-	-	psi-mi:"MI:0818"(molecular weight estimation by coomasie staining)	psi-mi:"MI:0818"(molecular weight estimation by coomasie staining)