#ID(s) interactor A ID(s) interactor B Alt. ID(s) interactor A Alt. ID(s) interactor B Alias(es) interactor A Alias(es) interactor B Interaction detection method(s) Publication 1st author(s) Publication Identifier(s) Taxid interactor A Taxid interactor B Interaction type(s) Source database(s) Interaction identifier(s) Confidence value(s) Expansion method(s) Biological role(s) interactor A Biological role(s) interactor B Experimental role(s) interactor A Experimental role(s) interactor B Type(s) interactor A Type(s) interactor B Xref(s) interactor A Xref(s) interactor B Interaction Xref(s) Annotation(s) interactor A Annotation(s) interactor B Interaction annotation(s) Host organism(s) Interaction parameter(s) Creation date Update date Checksum(s) interactor A Checksum(s) interactor B Interaction Checksum(s) Negative Feature(s) interactor A Feature(s) interactor B Stoichiometry(s) interactor A Stoichiometry(s) interactor B Identification method participant A Identification method participant B uniprotkb:P20810 intenz:3.4.22.52 intact:EBI-1268770|uniprotkb:G5E9D3|uniprotkb:O95360|uniprotkb:Q96D08|uniprotkb:Q9H1Z5|uniprotkb:Q7Z4K0|intact:EBI-11042429|uniprotkb:B7Z468|uniprotkb:Q05DE8|uniprotkb:G5E946|ensembl:ENSP00000339914|ensembl:ENSP00000379158 intact:EBI-9022969|wwpdb:1kfu|wwpdb:1kfx psi-mi:ical_human(display_long)|uniprotkb:CAST(gene name)|psi-mi:CAST(display_short)|uniprotkb:Calpain inhibitor(gene name synonym)|uniprotkb:Sperm BS-17 component(gene name synonym) psi-mi:capns1-capn2_human(display_short)|psi-mi:3.4.22.52(display_long)|intact:M-Calpain complex(complex recommended name)|intact:"CAPN2:CAPNS1"(complex systematic name)|intact:CAPN2/S1 complex(complex synonym)|intact:Conventional Calpain complex(complex synonym)|intact:Calpain-2 complex(complex synonym)|intact:CAPN2/CAPNS1 complex(complex synonym) psi-mi:"MI:0969"(bio-layer interferometry) Nguyen et al. (2016) pubmed:28319173|imex:IM-27050 taxid:9606(human)|taxid:9606(Homo sapiens) taxid:9606(human)|taxid:9606(Homo sapiens) psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-21446603|imex:IM-27050-1 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:1302"(stable complex) refseq:NP_001035905.1|refseq:XP_006714759.1|refseq:XP_006714766.1|refseq:NP_001271141.1|refseq:XP_006714764.1|refseq:NP_001035906.1|refseq:NP_001035907.1|refseq:NP_001035908.1|refseq:NP_001035909.1|refseq:NP_001317560.1|refseq:NP_001741.4|refseq:XP_016865402.1|go:"GO:2000675"(negative regulation of type B pancreatic cell apoptotic process)|interpro:IPR001259(Proteinase inhibitor I27, calpastatin)|interpro:IPR026998|mint:P20810|reactome:R-HSA-1474228|reactome:R-HSA-8862803|go:"GO:1990709"(presynaptic active zone organization)|ensembl:ENSG00000153113(gene)|ensembl:ENST00000341926(transcript)|ensembl:ENST00000395813(transcript)|go:"GO:0003723"(RNA binding)|go:"GO:0004866"(endopeptidase inhibitor activity)|go:"GO:0005737"(cytoplasm)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005829"(cytosol)|go:"GO:0010859"(calcium-dependent cysteine-type endopeptidase inhibitor activity)|go:"GO:0016020"(membrane)|go:"GO:0045296"(cadherin binding)|go:"GO:0097340"(inhibition of cysteine-type endopeptidase activity)|refseq:NP_775083.1|refseq:XP_006714767.1 go:"GO:0005509"(calcium ion binding)|go:"GO:0004198"(calcium-dependent cysteine-type endopeptidase activity)|go:"GO:0006508"(proteolysis)|evidence ontology:"ECO:0000353"|complex portal:CPX-2674(complex-primary)|pubmed:21864727(see-also)|efo:"Orphanet:98473"(see-also)|efo:"EFO:0001059"(see-also)|efo:"EFO:0000249"(see-also)|efo:"EFO:0002508"(see-also)|efo:"EFO:0000685"(see-also)|efo:"EFO:0002687"(see-also)|efo:"EFO:0009019"(see-also)|pubmed:10639123(see-also)|pubmed:22711986(see-also)|pubmed:9271093(see-also)|pubmed:21849499(see-also)|intact:EBI-1028987(exp-evidence)|go:"GO:0110158"(calpain complex) - - complex-properties:"Calpain (CAPN2/mCL) and its small regulatory subunit (CAPNS1/30K) bind via their respective 5th (of 5) EF-hand motifs. The N-terminus of CAPNS1 contains hydrophobic Gly-clusters, the Gly-rich domain. The catalytically-active cleft is rather deep and narrow which might explains calpain's preference for digesting inter-domain unstructured regions. It is formed by the two calpain protease core domains, PC1 and PC2, containing one Ca2+-binding sites per core. Calcium binding to several domains of both subunits releases the steric hindrance by allowing PC1 and PC2 to move closer to each other while the secondary structures remain essentially unchanged and may result in the dissociation of the small regulatory subunit from the catalytic subunits. Most other members of the calpain family of proteins do not require a regulatory subunit and are activated directly by calcium binding."|antagonist:"Calpastatin (CAST, P20810)"|ligand:"Calcium ion (Ca2+, CHEBI:29108)"|disease:"Autosomal recessive spastic paraplegia type 76 [EFO:0009019]: Spastic paraplegia-76 is an autosomal recessive neurologic disorder characterized by young-adult onset of slowly progressive spasticity of the lower limbs resulting in gait difficulties. Most affected individuals have upper limb involvement and additional features such as foot deformities and dysarthria. Cognition is unaffected."|disease:"Muscular dystrophy [Orphanet:98473]: Muscular dystrophy (MD) refers to a group of more than 30 genetic diseases characterized by progressive weakness and degeneration of the skeletal muscles that control movement."|disease:"Cataract [EFO:0001059]: Partial or complete opacity of the crystalline lens of one or both eyes that decreases visual acuity and eventually results in blindness. Some cataracts appear in infancy or in childhood, but most develop in older individuals."|disease:"Alzheimer's disease [EFO:0000249]: A progressive, neurodegenerative disease characterized by loss of function and death of nerve cells in several areas of the brain leading to loss of cognitive function such as memory and language."|disease:"Parkinson's disease [EFO:0002508]: A progressive degenerative disorder of the central nervous system characterized by loss of dopamine producing neurons in the substantia nigra and the presence of Lewy bodies in the substantia nigra and locus coeruleus. Signs and symptoms include tremor which is most pronounced during rest, muscle rigidity, slowing of the voluntary movements, a tendency to fall back, and a mask-like facial expression."|disease:"Rheumatoid arthritis [EFO:0000685]: A chronic, systemic autoimmune disorder characterized by inflammation in the synovial membranes and articular surfaces. It manifests primarily as a symmetric, erosive polyarthritis that spares the axial skeleton and is typically associated with the presence in the serum of rheumatoid factor."|disease:"Ischemia reperfusion injury [EFO:0002687]: Adverse functional, metabolic, or structural changes in ischemic tissues resulting from the restoration of blood flow to the tissue (reperfusion), including swelling; hemorrhage; necrosis; and damage from free radicals. The most common instance is myocardial reperfusion injury."|curated-complex:"A calcium-dependent protease complex that processes the substrate by limited proteolysis rather than degrading it. In some cases the proteolytic action activates the substrate, for example, it cleaves CDK5R1/p35 (Q15078) into its p25 form that is associated with Alzheimer's disease. Involved in cytoskeletal remodeling, signal transduction and implicated in cell cycle regulation and apoptosis. Finely-balanced calpain homeostasis is required as both over and under-activation causes disease. Calpain complexes recognise their substrates based on a short peptide sequence. Inhibited by the intrinsically-unstructured calpastatin (P20810) by its tight binding to the calpain catalytic subunit."|disease:"In general, under disease/damaged conditions, such as muscular dystrophy, cardiomyopathy, traumatic ischemia, or lissencephaly, the over-activation of conventional calpains (probably due to compromised intracellular Ca2+ homeostasis) has been identified as an exacerbating factor." figure legend:Figure 2b|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) kd:1.7x10^-9(mol) 2019/05/30 2019/06/06 rogid:sDc8/o13gLhr18fYsz/grYg13Lo9606 - - false sufficient binding region:137-277|glutathione s tranferase tag:n-n sufficient binding region:59-268|mutation:105-105 - - psi-mi:"MI:0396"(predetermined participant) psi-mi:"MI:0396"(predetermined participant) uniprotkb:P20810 intenz:3.4.22.52 intact:EBI-1268770|uniprotkb:G5E9D3|uniprotkb:O95360|uniprotkb:Q96D08|uniprotkb:Q9H1Z5|uniprotkb:Q7Z4K0|intact:EBI-11042429|uniprotkb:B7Z468|uniprotkb:Q05DE8|uniprotkb:G5E946|ensembl:ENSP00000339914|ensembl:ENSP00000379158 intact:EBI-9022969|wwpdb:1kfu|wwpdb:1kfx psi-mi:ical_human(display_long)|uniprotkb:CAST(gene name)|psi-mi:CAST(display_short)|uniprotkb:Calpain inhibitor(gene name synonym)|uniprotkb:Sperm BS-17 component(gene name synonym) psi-mi:capns1-capn2_human(display_short)|psi-mi:3.4.22.52(display_long)|intact:M-Calpain complex(complex recommended name)|intact:"CAPN2:CAPNS1"(complex systematic name)|intact:CAPN2/S1 complex(complex synonym)|intact:Conventional Calpain complex(complex synonym)|intact:Calpain-2 complex(complex synonym)|intact:CAPN2/CAPNS1 complex(complex synonym) psi-mi:"MI:0969"(bio-layer interferometry) Nguyen et al. (2016) pubmed:28319173|imex:IM-27050 taxid:9606(human)|taxid:9606(Homo sapiens) taxid:9606(human)|taxid:9606(Homo sapiens) psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-21446614|imex:IM-27050-3 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:1302"(stable complex) refseq:NP_001035905.1|refseq:XP_006714759.1|refseq:XP_006714766.1|refseq:NP_001271141.1|refseq:XP_006714764.1|refseq:NP_001035906.1|refseq:NP_001035907.1|refseq:NP_001035908.1|refseq:NP_001035909.1|refseq:NP_001317560.1|refseq:NP_001741.4|refseq:XP_016865402.1|go:"GO:2000675"(negative regulation of type B pancreatic cell apoptotic process)|interpro:IPR001259(Proteinase inhibitor I27, calpastatin)|interpro:IPR026998|mint:P20810|reactome:R-HSA-1474228|reactome:R-HSA-8862803|go:"GO:1990709"(presynaptic active zone organization)|ensembl:ENSG00000153113(gene)|ensembl:ENST00000341926(transcript)|ensembl:ENST00000395813(transcript)|go:"GO:0003723"(RNA binding)|go:"GO:0004866"(endopeptidase inhibitor activity)|go:"GO:0005737"(cytoplasm)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005829"(cytosol)|go:"GO:0010859"(calcium-dependent cysteine-type endopeptidase inhibitor activity)|go:"GO:0016020"(membrane)|go:"GO:0045296"(cadherin binding)|go:"GO:0097340"(inhibition of cysteine-type endopeptidase activity)|refseq:NP_775083.1|refseq:XP_006714767.1 go:"GO:0005509"(calcium ion binding)|go:"GO:0004198"(calcium-dependent cysteine-type endopeptidase activity)|go:"GO:0006508"(proteolysis)|evidence ontology:"ECO:0000353"|complex portal:CPX-2674(complex-primary)|pubmed:21864727(see-also)|efo:"Orphanet:98473"(see-also)|efo:"EFO:0001059"(see-also)|efo:"EFO:0000249"(see-also)|efo:"EFO:0002508"(see-also)|efo:"EFO:0000685"(see-also)|efo:"EFO:0002687"(see-also)|efo:"EFO:0009019"(see-also)|pubmed:10639123(see-also)|pubmed:22711986(see-also)|pubmed:9271093(see-also)|pubmed:21849499(see-also)|intact:EBI-1028987(exp-evidence)|go:"GO:0110158"(calpain complex) - - complex-properties:"Calpain (CAPN2/mCL) and its small regulatory subunit (CAPNS1/30K) bind via their respective 5th (of 5) EF-hand motifs. The N-terminus of CAPNS1 contains hydrophobic Gly-clusters, the Gly-rich domain. The catalytically-active cleft is rather deep and narrow which might explains calpain's preference for digesting inter-domain unstructured regions. It is formed by the two calpain protease core domains, PC1 and PC2, containing one Ca2+-binding sites per core. Calcium binding to several domains of both subunits releases the steric hindrance by allowing PC1 and PC2 to move closer to each other while the secondary structures remain essentially unchanged and may result in the dissociation of the small regulatory subunit from the catalytic subunits. Most other members of the calpain family of proteins do not require a regulatory subunit and are activated directly by calcium binding."|antagonist:"Calpastatin (CAST, P20810)"|ligand:"Calcium ion (Ca2+, CHEBI:29108)"|disease:"Autosomal recessive spastic paraplegia type 76 [EFO:0009019]: Spastic paraplegia-76 is an autosomal recessive neurologic disorder characterized by young-adult onset of slowly progressive spasticity of the lower limbs resulting in gait difficulties. Most affected individuals have upper limb involvement and additional features such as foot deformities and dysarthria. Cognition is unaffected."|disease:"Muscular dystrophy [Orphanet:98473]: Muscular dystrophy (MD) refers to a group of more than 30 genetic diseases characterized by progressive weakness and degeneration of the skeletal muscles that control movement."|disease:"Cataract [EFO:0001059]: Partial or complete opacity of the crystalline lens of one or both eyes that decreases visual acuity and eventually results in blindness. Some cataracts appear in infancy or in childhood, but most develop in older individuals."|disease:"Alzheimer's disease [EFO:0000249]: A progressive, neurodegenerative disease characterized by loss of function and death of nerve cells in several areas of the brain leading to loss of cognitive function such as memory and language."|disease:"Parkinson's disease [EFO:0002508]: A progressive degenerative disorder of the central nervous system characterized by loss of dopamine producing neurons in the substantia nigra and the presence of Lewy bodies in the substantia nigra and locus coeruleus. Signs and symptoms include tremor which is most pronounced during rest, muscle rigidity, slowing of the voluntary movements, a tendency to fall back, and a mask-like facial expression."|disease:"Rheumatoid arthritis [EFO:0000685]: A chronic, systemic autoimmune disorder characterized by inflammation in the synovial membranes and articular surfaces. It manifests primarily as a symmetric, erosive polyarthritis that spares the axial skeleton and is typically associated with the presence in the serum of rheumatoid factor."|disease:"Ischemia reperfusion injury [EFO:0002687]: Adverse functional, metabolic, or structural changes in ischemic tissues resulting from the restoration of blood flow to the tissue (reperfusion), including swelling; hemorrhage; necrosis; and damage from free radicals. The most common instance is myocardial reperfusion injury."|curated-complex:"A calcium-dependent protease complex that processes the substrate by limited proteolysis rather than degrading it. In some cases the proteolytic action activates the substrate, for example, it cleaves CDK5R1/p35 (Q15078) into its p25 form that is associated with Alzheimer's disease. Involved in cytoskeletal remodeling, signal transduction and implicated in cell cycle regulation and apoptosis. Finely-balanced calpain homeostasis is required as both over and under-activation causes disease. Calpain complexes recognise their substrates based on a short peptide sequence. Inhibited by the intrinsically-unstructured calpastatin (P20810) by its tight binding to the calpain catalytic subunit."|disease:"In general, under disease/damaged conditions, such as muscular dystrophy, cardiomyopathy, traumatic ischemia, or lissencephaly, the over-activation of conventional calpains (probably due to compromised intracellular Ca2+ homeostasis) has been identified as an exacerbating factor." figure legend:Figure 2a|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2019/05/30 2019/06/06 rogid:sDc8/o13gLhr18fYsz/grYg13Lo9606 - - false sufficient binding region:137-277|biotin tag:?-? sufficient binding region:59-268|mutation:105-105 - - psi-mi:"MI:0396"(predetermined participant) psi-mi:"MI:0396"(predetermined participant) uniprotkb:P04632 uniprotkb:P17655 intact:EBI-711828|uniprotkb:Q8WTX3|uniprotkb:Q96EW0|uniprotkb:A8K0P1|ensembl:ENSP00000246533|ensembl:ENSP00000464849 intact:EBI-1028956|uniprotkb:Q16738|uniprotkb:Q6PJT3|uniprotkb:Q8WU26|uniprotkb:Q9HBB1|uniprotkb:A6NDG7|ensembl:ENSP00000295006|uniprotkb:B7ZA96|uniprotkb:E7ES58 psi-mi:cpns1_human(display_long)|uniprotkb:Calcium-dependent protease small subunit 1(gene name synonym)|uniprotkb:Calcium-dependent protease small subunit(gene name synonym)|uniprotkb:Calpain regulatory subunit(gene name synonym)|uniprotkb:Calcium-activated neutral proteinase small subunit(gene name synonym)|uniprotkb:CAPNS1(gene name)|psi-mi:CAPNS1(display_short)|uniprotkb:CAPN4(gene name synonym)|uniprotkb:CAPNS(gene name synonym) psi-mi:can2_human(display_long)|uniprotkb:CAPN2(gene name)|psi-mi:CAPN2(display_short)|uniprotkb:CANPL2(gene name synonym)|uniprotkb:Calpain-2 large subunit(gene name synonym)|uniprotkb:Calcium-activated neutral proteinase 2(gene name synonym)|uniprotkb:Calpain M-type(gene name synonym)|uniprotkb:Millimolar-calpain(gene name synonym)|uniprotkb:Calpain large polypeptide L2(gene name synonym) psi-mi:"MI:0091"(chromatography technology) Nguyen et al. (2016) pubmed:28319173|imex:IM-27050 taxid:9606(human)|taxid:9606(Homo sapiens) taxid:9606(human)|taxid:9606(Homo sapiens) psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-21446725|imex:IM-27050-5 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0498"(prey) psi-mi:"MI:0496"(bait) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) ensembl:ENSG00000126247(gene)|ensembl:ENST00000246533(transcript)|ensembl:ENST00000588815(transcript)|go:"GO:0004198"(calcium-dependent cysteine-type endopeptidase activity)|go:"GO:0005509"(calcium ion binding)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0008284"(positive regulation of cell population proliferation)|go:"GO:0016020"(membrane)|go:"GO:0016241"(regulation of macroautophagy)|go:"GO:0070062"(extracellular exosome)|interpro:IPR002048(Calcium-binding EF-hand)|interpro:IPR011992(EF-Hand type)|interpro:IPR018247|interpro:IPR029642|mint:P04632|rcsb pdb:1KFU|rcsb pdb:1KFX|rcsb pdb:4PHJ|rcsb pdb:4PHK|rcsb pdb:4PHM|rcsb pdb:4WQ2|rcsb pdb:4WQ3|rcsb pdb:5D69|rcsb pdb:6QLB|reactome:R-HSA-1474228|reactome:R-HSA-6809371|reactome:R-HSA-8862803|refseq:NP_001003962.1|refseq:NP_001740.1|refseq:NP_001289561.1|refseq:NP_001289562.1|refseq:XP_005259352.1|refseq:XP_005259353.1 refseq:NP_001139540.1|refseq:NP_001739.2|ensembl:ENSG00000162909(gene)|ensembl:ENST00000295006(transcript)|go:"GO:0000785"(chromatin)|go:"GO:0001666"(response to hypoxia)|go:"GO:0001824"(blastocyst development)|go:"GO:0004198"(calcium-dependent cysteine-type endopeptidase activity)|go:"GO:0005509"(calcium ion binding)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0005925"(focal adhesion)|go:"GO:0006508"(proteolysis)|go:"GO:0007520"(myoblast fusion)|go:"GO:0007565"(female pregnancy)|go:"GO:0008092"(cytoskeletal protein binding)|go:"GO:0008234"(cysteine-type peptidase activity)|go:"GO:0009897"(external side of plasma membrane)|go:"GO:0010666"(positive regulation of cardiac muscle cell apoptotic process)|go:"GO:0016540"(protein autoprocessing)|go:"GO:0019899"(enzyme binding)|go:"GO:0030425"(dendrite)|go:"GO:0030864"(cortical actin cytoskeleton)|go:"GO:0031143"(pseudopodium)|go:"GO:0032675"(regulation of interleukin-6 production)|go:"GO:0035458"(cellular response to interferon-beta)|go:"GO:0042542"(response to hydrogen peroxide)|go:"GO:0005634"(nucleus)|go:"GO:0043025"(neuronal cell body)|go:"GO:0044877"(protein-containing complex binding)|go:"GO:0045121"(membrane raft)|go:"GO:0048266"(behavioral response to pain)|go:"GO:0051493"(regulation of cytoskeleton organization)|go:"GO:0051603"(proteolysis involved in cellular protein catabolic process)|go:"GO:0070062"(extracellular exosome)|go:"GO:0071222"(cellular response to lipopolysaccharide)|go:"GO:0071230"(cellular response to amino acid stimulus)|go:"GO:0097038"(perinuclear endoplasmic reticulum)|go:"GO:1901216"(positive regulation of neuron death)|go:"GO:1901741"(positive regulation of myoblast fusion)|go:"GO:2001247"(positive regulation of phosphatidylcholine biosynthetic process)|interpro:IPR000169(Peptidase, cysteine peptidase active site)|interpro:IPR001300(Peptidase C2, calpain)|go:"GO:0005737"(cytoplasm)|go:"GO:0005758"(mitochondrial intermembrane space)|go:"GO:0005764"(lysosome)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005794"(Golgi apparatus)|interpro:IPR011992(EF-Hand type)|interpro:IPR018247|interpro:IPR022682|interpro:IPR022683|interpro:IPR022684|interpro:IPR029539|interpro:IPR033883|interpro:IPR036213|interpro:IPR038765|interpro:IPR042736|mint:P17655|rcsb pdb:1KFU|rcsb pdb:1KFX|rcsb pdb:2NQA|reactome:R-HSA-1474228|reactome:R-HSA-8862803|interpro:IPR002048(Calcium-binding EF-hand) - - - figure legend:Figure 3a, 3b|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)") - 2019/05/30 2019/06/03 rogid:siQugDvaWq0YjOr8qOCf0BsRRKs9606 rogid:r60XX66kihGVLaa9888soeXjc0M9606 rigid:kxJ08WWQWJqxYGcaFqt/aFiR9eI false sufficient binding region:59-268 mutation:105-105|his tag:?-? - - psi-mi:"MI:0816"(molecular weight estimation by staining) psi-mi:"MI:0816"(molecular weight estimation by staining) uniprotkb:P20810 uniprotkb:P04632 intact:EBI-1268770|uniprotkb:G5E9D3|uniprotkb:O95360|uniprotkb:Q96D08|uniprotkb:Q9H1Z5|uniprotkb:Q7Z4K0|intact:EBI-11042429|uniprotkb:B7Z468|uniprotkb:Q05DE8|uniprotkb:G5E946|ensembl:ENSP00000339914|ensembl:ENSP00000379158 intact:EBI-711828|uniprotkb:Q8WTX3|uniprotkb:Q96EW0|uniprotkb:A8K0P1|ensembl:ENSP00000246533|ensembl:ENSP00000464849 psi-mi:ical_human(display_long)|uniprotkb:CAST(gene name)|psi-mi:CAST(display_short)|uniprotkb:Calpain inhibitor(gene name synonym)|uniprotkb:Sperm BS-17 component(gene name synonym) psi-mi:cpns1_human(display_long)|uniprotkb:Calcium-dependent protease small subunit 1(gene name synonym)|uniprotkb:Calcium-dependent protease small subunit(gene name synonym)|uniprotkb:Calpain regulatory subunit(gene name synonym)|uniprotkb:Calcium-activated neutral proteinase small subunit(gene name synonym)|uniprotkb:CAPNS1(gene name)|psi-mi:CAPNS1(display_short)|uniprotkb:CAPN4(gene name synonym)|uniprotkb:CAPNS(gene name synonym) psi-mi:"MI:0096"(pull down) Nguyen et al. (2016) pubmed:28319173|imex:IM-27050 taxid:9606(human)|taxid:9606(Homo sapiens) taxid:9606(human)|taxid:9606(Homo sapiens) psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-21446789|imex:IM-27050-7 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) refseq:NP_001035905.1|refseq:XP_006714759.1|refseq:XP_006714766.1|refseq:NP_001271141.1|refseq:XP_006714764.1|refseq:NP_001035906.1|refseq:NP_001035907.1|refseq:NP_001035908.1|refseq:NP_001035909.1|refseq:NP_001317560.1|refseq:NP_001741.4|refseq:XP_016865402.1|go:"GO:2000675"(negative regulation of type B pancreatic cell apoptotic process)|interpro:IPR001259(Proteinase inhibitor I27, calpastatin)|interpro:IPR026998|mint:P20810|reactome:R-HSA-1474228|reactome:R-HSA-8862803|go:"GO:1990709"(presynaptic active zone organization)|ensembl:ENSG00000153113(gene)|ensembl:ENST00000341926(transcript)|ensembl:ENST00000395813(transcript)|go:"GO:0003723"(RNA binding)|go:"GO:0004866"(endopeptidase inhibitor activity)|go:"GO:0005737"(cytoplasm)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005829"(cytosol)|go:"GO:0010859"(calcium-dependent cysteine-type endopeptidase inhibitor activity)|go:"GO:0016020"(membrane)|go:"GO:0045296"(cadherin binding)|go:"GO:0097340"(inhibition of cysteine-type endopeptidase activity)|refseq:NP_775083.1|refseq:XP_006714767.1 ensembl:ENSG00000126247(gene)|ensembl:ENST00000246533(transcript)|ensembl:ENST00000588815(transcript)|go:"GO:0004198"(calcium-dependent cysteine-type endopeptidase activity)|go:"GO:0005509"(calcium ion binding)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0008284"(positive regulation of cell population proliferation)|go:"GO:0016020"(membrane)|go:"GO:0016241"(regulation of macroautophagy)|go:"GO:0070062"(extracellular exosome)|interpro:IPR002048(Calcium-binding EF-hand)|interpro:IPR011992(EF-Hand type)|interpro:IPR018247|interpro:IPR029642|mint:P04632|rcsb pdb:1KFU|rcsb pdb:1KFX|rcsb pdb:4PHJ|rcsb pdb:4PHK|rcsb pdb:4PHM|rcsb pdb:4WQ2|rcsb pdb:4WQ3|rcsb pdb:5D69|rcsb pdb:6QLB|reactome:R-HSA-1474228|reactome:R-HSA-6809371|reactome:R-HSA-8862803|refseq:NP_001003962.1|refseq:NP_001740.1|refseq:NP_001289561.1|refseq:NP_001289562.1|refseq:XP_005259352.1|refseq:XP_005259353.1 - - - figure legend:Figure 3a, 3b|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2019/05/30 2019/06/03 rogid:sDc8/o13gLhr18fYsz/grYg13Lo9606 rogid:siQugDvaWq0YjOr8qOCf0BsRRKs9606 rigid:MXCd7yyvcnvvFY+7LVYXkncbyX0 false sufficient binding region:137-277|biotin tag:?-? sufficient binding region:59-268 - - psi-mi:"MI:0815"(confirmation by molecular weight) psi-mi:"MI:0815"(confirmation by molecular weight) uniprotkb:P20810 uniprotkb:P17655 intact:EBI-1268770|uniprotkb:G5E9D3|uniprotkb:O95360|uniprotkb:Q96D08|uniprotkb:Q9H1Z5|uniprotkb:Q7Z4K0|intact:EBI-11042429|uniprotkb:B7Z468|uniprotkb:Q05DE8|uniprotkb:G5E946|ensembl:ENSP00000339914|ensembl:ENSP00000379158 intact:EBI-1028956|uniprotkb:Q16738|uniprotkb:Q6PJT3|uniprotkb:Q8WU26|uniprotkb:Q9HBB1|uniprotkb:A6NDG7|ensembl:ENSP00000295006|uniprotkb:B7ZA96|uniprotkb:E7ES58 psi-mi:ical_human(display_long)|uniprotkb:CAST(gene name)|psi-mi:CAST(display_short)|uniprotkb:Calpain inhibitor(gene name synonym)|uniprotkb:Sperm BS-17 component(gene name synonym) psi-mi:can2_human(display_long)|uniprotkb:CAPN2(gene name)|psi-mi:CAPN2(display_short)|uniprotkb:CANPL2(gene name synonym)|uniprotkb:Calpain-2 large subunit(gene name synonym)|uniprotkb:Calcium-activated neutral proteinase 2(gene name synonym)|uniprotkb:Calpain M-type(gene name synonym)|uniprotkb:Millimolar-calpain(gene name synonym)|uniprotkb:Calpain large polypeptide L2(gene name synonym) psi-mi:"MI:0096"(pull down) Nguyen et al. (2016) pubmed:28319173|imex:IM-27050 taxid:9606(human)|taxid:9606(Homo sapiens) taxid:9606(human)|taxid:9606(Homo sapiens) psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-21446789|imex:IM-27050-7 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) refseq:NP_001035905.1|refseq:XP_006714759.1|refseq:XP_006714766.1|refseq:NP_001271141.1|refseq:XP_006714764.1|refseq:NP_001035906.1|refseq:NP_001035907.1|refseq:NP_001035908.1|refseq:NP_001035909.1|refseq:NP_001317560.1|refseq:NP_001741.4|refseq:XP_016865402.1|go:"GO:2000675"(negative regulation of type B pancreatic cell apoptotic process)|interpro:IPR001259(Proteinase inhibitor I27, calpastatin)|interpro:IPR026998|mint:P20810|reactome:R-HSA-1474228|reactome:R-HSA-8862803|go:"GO:1990709"(presynaptic active zone organization)|ensembl:ENSG00000153113(gene)|ensembl:ENST00000341926(transcript)|ensembl:ENST00000395813(transcript)|go:"GO:0003723"(RNA binding)|go:"GO:0004866"(endopeptidase inhibitor activity)|go:"GO:0005737"(cytoplasm)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005829"(cytosol)|go:"GO:0010859"(calcium-dependent cysteine-type endopeptidase inhibitor activity)|go:"GO:0016020"(membrane)|go:"GO:0045296"(cadherin binding)|go:"GO:0097340"(inhibition of cysteine-type endopeptidase activity)|refseq:NP_775083.1|refseq:XP_006714767.1 refseq:NP_001139540.1|refseq:NP_001739.2|ensembl:ENSG00000162909(gene)|ensembl:ENST00000295006(transcript)|go:"GO:0000785"(chromatin)|go:"GO:0001666"(response to hypoxia)|go:"GO:0001824"(blastocyst development)|go:"GO:0004198"(calcium-dependent cysteine-type endopeptidase activity)|go:"GO:0005509"(calcium ion binding)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0005925"(focal adhesion)|go:"GO:0006508"(proteolysis)|go:"GO:0007520"(myoblast fusion)|go:"GO:0007565"(female pregnancy)|go:"GO:0008092"(cytoskeletal protein binding)|go:"GO:0008234"(cysteine-type peptidase activity)|go:"GO:0009897"(external side of plasma membrane)|go:"GO:0010666"(positive regulation of cardiac muscle cell apoptotic process)|go:"GO:0016540"(protein autoprocessing)|go:"GO:0019899"(enzyme binding)|go:"GO:0030425"(dendrite)|go:"GO:0030864"(cortical actin cytoskeleton)|go:"GO:0031143"(pseudopodium)|go:"GO:0032675"(regulation of interleukin-6 production)|go:"GO:0035458"(cellular response to interferon-beta)|go:"GO:0042542"(response to hydrogen peroxide)|go:"GO:0005634"(nucleus)|go:"GO:0043025"(neuronal cell body)|go:"GO:0044877"(protein-containing complex binding)|go:"GO:0045121"(membrane raft)|go:"GO:0048266"(behavioral response to pain)|go:"GO:0051493"(regulation of cytoskeleton organization)|go:"GO:0051603"(proteolysis involved in cellular protein catabolic process)|go:"GO:0070062"(extracellular exosome)|go:"GO:0071222"(cellular response to lipopolysaccharide)|go:"GO:0071230"(cellular response to amino acid stimulus)|go:"GO:0097038"(perinuclear endoplasmic reticulum)|go:"GO:1901216"(positive regulation of neuron death)|go:"GO:1901741"(positive regulation of myoblast fusion)|go:"GO:2001247"(positive regulation of phosphatidylcholine biosynthetic process)|interpro:IPR000169(Peptidase, cysteine peptidase active site)|interpro:IPR001300(Peptidase C2, calpain)|go:"GO:0005737"(cytoplasm)|go:"GO:0005758"(mitochondrial intermembrane space)|go:"GO:0005764"(lysosome)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005794"(Golgi apparatus)|interpro:IPR011992(EF-Hand type)|interpro:IPR018247|interpro:IPR022682|interpro:IPR022683|interpro:IPR022684|interpro:IPR029539|interpro:IPR033883|interpro:IPR036213|interpro:IPR038765|interpro:IPR042736|mint:P17655|rcsb pdb:1KFU|rcsb pdb:1KFX|rcsb pdb:2NQA|reactome:R-HSA-1474228|reactome:R-HSA-8862803|interpro:IPR002048(Calcium-binding EF-hand) - - - figure legend:Figure 3a, 3b|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2019/05/30 2019/06/03 rogid:sDc8/o13gLhr18fYsz/grYg13Lo9606 rogid:r60XX66kihGVLaa9888soeXjc0M9606 rigid:MXCd7yyvcnvvFY+7LVYXkncbyX0 false sufficient binding region:137-277|biotin tag:?-? mutation:105-105 - - psi-mi:"MI:0815"(confirmation by molecular weight) psi-mi:"MI:0815"(confirmation by molecular weight) uniprotkb:P17655 uniprotkb:P04632 intact:EBI-1028956|uniprotkb:Q16738|uniprotkb:Q6PJT3|uniprotkb:Q8WU26|uniprotkb:Q9HBB1|uniprotkb:A6NDG7|ensembl:ENSP00000295006|uniprotkb:B7ZA96|uniprotkb:E7ES58 intact:EBI-711828|uniprotkb:Q8WTX3|uniprotkb:Q96EW0|uniprotkb:A8K0P1|ensembl:ENSP00000246533|ensembl:ENSP00000464849 psi-mi:can2_human(display_long)|uniprotkb:CAPN2(gene name)|psi-mi:CAPN2(display_short)|uniprotkb:CANPL2(gene name synonym)|uniprotkb:Calpain-2 large subunit(gene name synonym)|uniprotkb:Calcium-activated neutral proteinase 2(gene name synonym)|uniprotkb:Calpain M-type(gene name synonym)|uniprotkb:Millimolar-calpain(gene name synonym)|uniprotkb:Calpain large polypeptide L2(gene name synonym) psi-mi:cpns1_human(display_long)|uniprotkb:Calcium-dependent protease small subunit 1(gene name synonym)|uniprotkb:Calcium-dependent protease small subunit(gene name synonym)|uniprotkb:Calpain regulatory subunit(gene name synonym)|uniprotkb:Calcium-activated neutral proteinase small subunit(gene name synonym)|uniprotkb:CAPNS1(gene name)|psi-mi:CAPNS1(display_short)|uniprotkb:CAPN4(gene name synonym)|uniprotkb:CAPNS(gene name synonym) psi-mi:"MI:0016"(circular dichroism) Nguyen et al. (2016) pubmed:28319173|imex:IM-27050 taxid:9606(human)|taxid:9606(Homo sapiens) taxid:9606(human)|taxid:9606(Homo sapiens) psi-mi:"MI:0407"(direct interaction) psi-mi:"MI:0469"(IntAct) intact:EBI-21449057|imex:IM-27050-8 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) refseq:NP_001139540.1|refseq:NP_001739.2|ensembl:ENSG00000162909(gene)|ensembl:ENST00000295006(transcript)|go:"GO:0000785"(chromatin)|go:"GO:0001666"(response to hypoxia)|go:"GO:0001824"(blastocyst development)|go:"GO:0004198"(calcium-dependent cysteine-type endopeptidase activity)|go:"GO:0005509"(calcium ion binding)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0005925"(focal adhesion)|go:"GO:0006508"(proteolysis)|go:"GO:0007520"(myoblast fusion)|go:"GO:0007565"(female pregnancy)|go:"GO:0008092"(cytoskeletal protein binding)|go:"GO:0008234"(cysteine-type peptidase activity)|go:"GO:0009897"(external side of plasma membrane)|go:"GO:0010666"(positive regulation of cardiac muscle cell apoptotic process)|go:"GO:0016540"(protein autoprocessing)|go:"GO:0019899"(enzyme binding)|go:"GO:0030425"(dendrite)|go:"GO:0030864"(cortical actin cytoskeleton)|go:"GO:0031143"(pseudopodium)|go:"GO:0032675"(regulation of interleukin-6 production)|go:"GO:0035458"(cellular response to interferon-beta)|go:"GO:0042542"(response to hydrogen peroxide)|go:"GO:0005634"(nucleus)|go:"GO:0043025"(neuronal cell body)|go:"GO:0044877"(protein-containing complex binding)|go:"GO:0045121"(membrane raft)|go:"GO:0048266"(behavioral response to pain)|go:"GO:0051493"(regulation of cytoskeleton organization)|go:"GO:0051603"(proteolysis involved in cellular protein catabolic process)|go:"GO:0070062"(extracellular exosome)|go:"GO:0071222"(cellular response to lipopolysaccharide)|go:"GO:0071230"(cellular response to amino acid stimulus)|go:"GO:0097038"(perinuclear endoplasmic reticulum)|go:"GO:1901216"(positive regulation of neuron death)|go:"GO:1901741"(positive regulation of myoblast fusion)|go:"GO:2001247"(positive regulation of phosphatidylcholine biosynthetic process)|interpro:IPR000169(Peptidase, cysteine peptidase active site)|interpro:IPR001300(Peptidase C2, calpain)|go:"GO:0005737"(cytoplasm)|go:"GO:0005758"(mitochondrial intermembrane space)|go:"GO:0005764"(lysosome)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005794"(Golgi apparatus)|interpro:IPR011992(EF-Hand type)|interpro:IPR018247|interpro:IPR022682|interpro:IPR022683|interpro:IPR022684|interpro:IPR029539|interpro:IPR033883|interpro:IPR036213|interpro:IPR038765|interpro:IPR042736|mint:P17655|rcsb pdb:1KFU|rcsb pdb:1KFX|rcsb pdb:2NQA|reactome:R-HSA-1474228|reactome:R-HSA-8862803|interpro:IPR002048(Calcium-binding EF-hand) ensembl:ENSG00000126247(gene)|ensembl:ENST00000246533(transcript)|ensembl:ENST00000588815(transcript)|go:"GO:0004198"(calcium-dependent cysteine-type endopeptidase activity)|go:"GO:0005509"(calcium ion binding)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0008284"(positive regulation of cell population proliferation)|go:"GO:0016020"(membrane)|go:"GO:0016241"(regulation of macroautophagy)|go:"GO:0070062"(extracellular exosome)|interpro:IPR002048(Calcium-binding EF-hand)|interpro:IPR011992(EF-Hand type)|interpro:IPR018247|interpro:IPR029642|mint:P04632|rcsb pdb:1KFU|rcsb pdb:1KFX|rcsb pdb:4PHJ|rcsb pdb:4PHK|rcsb pdb:4PHM|rcsb pdb:4WQ2|rcsb pdb:4WQ3|rcsb pdb:5D69|rcsb pdb:6QLB|reactome:R-HSA-1474228|reactome:R-HSA-6809371|reactome:R-HSA-8862803|refseq:NP_001003962.1|refseq:NP_001740.1|refseq:NP_001289561.1|refseq:NP_001289562.1|refseq:XP_005259352.1|refseq:XP_005259353.1 - - - figure legend:Figure 5|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2019/05/30 2019/06/06 rogid:r60XX66kihGVLaa9888soeXjc0M9606 rogid:siQugDvaWq0YjOr8qOCf0BsRRKs9606 rigid:kxJ08WWQWJqxYGcaFqt/aFiR9eI false mutation:105-105|his tag:?-? sufficient binding region:59-268 - - psi-mi:"MI:0396"(predetermined participant) psi-mi:"MI:0396"(predetermined participant)