#ID(s) interactor A	ID(s) interactor B	Alt. ID(s) interactor A	Alt. ID(s) interactor B	Alias(es) interactor A	Alias(es) interactor B	Interaction detection method(s)	Publication 1st author(s)	Publication Identifier(s)	Taxid interactor A	Taxid interactor B	Interaction type(s)	Source database(s)	Interaction identifier(s)	Confidence value(s)	Expansion method(s)	Biological role(s) interactor A	Biological role(s) interactor B	Experimental role(s) interactor A	Experimental role(s) interactor B	Type(s) interactor A	Type(s) interactor B	Xref(s) interactor A	Xref(s) interactor B	Interaction Xref(s)	Annotation(s) interactor A	Annotation(s) interactor B	Interaction annotation(s)	Host organism(s)	Interaction parameter(s)	Creation date	Update date	Checksum(s) interactor A	Checksum(s) interactor B	Interaction Checksum(s)	Negative	Feature(s) interactor A	Feature(s) interactor B	Stoichiometry(s) interactor A	Stoichiometry(s) interactor B	Identification method participant A	Identification method participant B
uniprotkb:Q63373-3	uniprotkb:Q62765	intact:EBI-20994045	intact:EBI-7281118|intact:MINT-1526995|ensembl:ENSRNOP00000075793	psi-mi:q63373-3(display_long)|uniprotkb:Neurexin I-beta(gene name synonym)|uniprotkb:Beta-4B5A(isoform synonym)|uniprotkb:Nrxn1(gene name)|psi-mi:Nrxn1(display_short)	psi-mi:nlgn1_rat(display_long)|uniprotkb:Nlgn1(gene name)|psi-mi:Nlgn1(display_short)|uniprotkb:Neuroligin I(gene name synonym)	psi-mi:"MI:0107"(surface plasmon resonance)	Comoletti et al. (2003)	pubmed:14522992|imex:IM-26884	taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)")	taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)")	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0469"(IntAct)	intact:EBI-21446562|imex:IM-26884-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	intact:EBI-1780696(isoform-parent)	rgd:621117|refseq:NP_446320.1|refseq:XP_017446075.1|refseq:XP_017446076.1|refseq:XP_017446077.1|refseq:XP_017446078.1|refseq:XP_017446079.1|refseq:XP_017446072.1|refseq:XP_017446073.1|refseq:XP_017446074.1|ensembl:ENSRNOG00000032576(gene)|ensembl:ENSRNOT00000092660(transcript)|go:"GO:0002087"(regulation of respiratory gaseous exchange by nervous system process)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005886"(plasma membrane)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0006605"(protein targeting)|go:"GO:0007157"(heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules)|go:"GO:0007158"(neuron cell-cell adhesion)|go:"GO:0007268"(chemical synaptic transmission)|go:"GO:0007399"(nervous system development)|go:"GO:0007416"(synapse assembly)|go:"GO:0009897"(external side of plasma membrane)|go:"GO:0009986"(cell surface)|go:"GO:0010841"(positive regulation of circadian sleep/wake cycle, wakefulness)|go:"GO:0014069"(postsynaptic density)|go:"GO:0016080"(synaptic vesicle targeting)|go:"GO:0016339"(calcium-dependent cell-cell adhesion via plasma membrane cell adhesion molecules)|go:"GO:0030165"(PDZ domain binding)|go:"GO:0030425"(dendrite)|go:"GO:0031175"(neuron projection development)|go:"GO:0032230"(positive regulation of synaptic transmission, GABAergic)|go:"GO:0032433"(filopodium tip)|go:"GO:0035418"(protein localization to synapse)|go:"GO:0038023"(signaling receptor activity)|go:"GO:0042043"(neurexin family protein binding)|go:"GO:0043083"(synaptic cleft)|go:"GO:0043197"(dendritic spine)|go:"GO:0043198"(dendritic shaft)|go:"GO:0043235"(receptor complex)|go:"GO:0044877"(protein-containing complex binding)|go:"GO:0045161"(neuronal ion channel clustering)|go:"GO:0045184"(establishment of protein localization)|go:"GO:0045202"(synapse)|go:"GO:0045664"(regulation of neuron differentiation)|go:"GO:0048488"(synaptic vesicle endocytosis)|go:"GO:0048489"(synaptic vesicle transport)|go:"GO:0042802"(identical protein binding)|go:"GO:0048511"(rhythmic process)|go:"GO:0048789"(cytoskeletal matrix organization at active zone)|go:"GO:0050804"(modulation of chemical synaptic transmission)|go:"GO:0050808"(synapse organization)|go:"GO:0050839"(cell adhesion molecule binding)|go:"GO:0051491"(positive regulation of filopodium assembly)|go:"GO:0051965"(positive regulation of synapse assembly)|go:"GO:0051968"(positive regulation of synaptic transmission, glutamatergic)|go:"GO:0060076"(excitatory synapse)|go:"GO:0060291"(long-term synaptic potentiation)|go:"GO:0060999"(positive regulation of dendritic spine development)|go:"GO:0061002"(negative regulation of dendritic spine morphogenesis)|go:"GO:0071277"(cellular response to calcium ion)|go:"GO:0072553"(terminal button organization)|go:"GO:0097091"(synaptic vesicle clustering)|go:"GO:0097104"(postsynaptic membrane assembly)|go:"GO:0097105"(presynaptic membrane assembly)|go:"GO:0097110"(scaffold protein binding)|go:"GO:0097113"(AMPA glutamate receptor clustering)|go:"GO:0097114"(NMDA glutamate receptor clustering)|go:"GO:0097115"(neurexin clustering involved in presynaptic membrane assembly)|go:"GO:0097119"(postsynaptic density protein 95 clustering)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098698"(postsynaptic specialization assembly)|go:"GO:0098793"(presynapse)|go:"GO:0098942"(retrograde trans-synaptic signaling by trans-synaptic protein complex)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099060"(integral component of postsynaptic specialization membrane)|go:"GO:0099174"(regulation of presynapse organization)|go:"GO:2000809"(positive regulation of synaptic vesicle clustering)|go:"GO:0099560"(synaptic membrane adhesion)|go:"GO:0140058"(neuron projection arborization)|interpro:IPR000460(Neuroligin)|interpro:IPR002018(Carboxylesterase, type B)|interpro:IPR019819|interpro:IPR029058|interpro:IPR030022|go:"GO:1900029"(positive regulation of ruffle assembly)|go:"GO:1900244"(positive regulation of synaptic vesicle endocytosis)|go:"GO:1902474"(positive regulation of protein localization to synapse)|go:"GO:1902533"(positive regulation of intracellular signal transduction)|go:"GO:1904861"(excitatory synapse assembly)|go:"GO:1905520"(positive regulation of presynaptic active zone assembly)|go:"GO:2000302"(positive regulation of synaptic vesicle exocytosis)|go:"GO:2000310"(regulation of NMDA receptor activity)|rcsb pdb:3BIW|go:"GO:2000311"(regulation of AMPA receptor activity)|go:"GO:2000463"(positive regulation of excitatory postsynaptic potential)|rcsb pdb:3BIX|rcsb pdb:3VKF|reactome:R-RNO-6794361|dip:DIP-44832N	-	-	comment:mint|function:"Neuronal cell surface protein thought to be involved in cell-cell-interactions by forming intercellular junctions through binding to beta-neurexins. Seems to play role in formation or maintenance of synaptic junctions. In vitro, triggers the de novo formation of presynaptic structures (By similarity). May be involved in specification of excitatory synapses"	figure legend:Table 1, Fig. 4, 5, 6, 7, 8, 9A and 10B|inhibition:"CHEBI:64755 - EDTA(2−)"|comment:To compare the relative NX1binding of the dif-ferent NL1 mutants, a range of NL1 concentrations extendingbetween 62.5 and 2000 nMwas injected and binding was mon-itored by SPR|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2019/02/11	2020/02/18	rogid:voabMMncKJwjAiQ4kdUcGukdtUw10116	rogid:I6UajYCOkz8f+3nt2IHH2rOCby810116	-	false	fc-igg tag:c-c	sufficient binding region:46-638|flag tag:n-n|N-glycosylated residue:303-303|mutation with no effect:343-343|mutation with no effect:111-111|mutation with no effect:549-549|mutation decreasing interaction strength:303-303	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
chebi:"CHEBI:29108"	uniprotkb:Q63373-3	intact:EBI-1169353	intact:EBI-20994045	psi-mi:"calcium(2+)"(display_short)|psi-mi:"CHEBI:29108"(display_long)	psi-mi:q63373-3(display_long)|uniprotkb:Neurexin I-beta(gene name synonym)|uniprotkb:Beta-4B5A(isoform synonym)|uniprotkb:Nrxn1(gene name)|psi-mi:Nrxn1(display_short)	psi-mi:"MI:0107"(surface plasmon resonance)	Comoletti et al. (2003)	pubmed:14522992|imex:IM-26884	taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)")	taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)")	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0469"(IntAct)	intact:EBI-21446562|imex:IM-26884-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0682"(cofactor)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0328"(small molecule)	psi-mi:"MI:0326"(protein)	-	intact:EBI-1780696(isoform-parent)	-	-	-	figure legend:Table 1, Fig. 4, 5, 6, 7, 8, 9A and 10B|inhibition:"CHEBI:64755 - EDTA(2−)"|comment:To compare the relative NX1binding of the dif-ferent NL1 mutants, a range of NL1 concentrations extendingbetween 62.5 and 2000 nMwas injected and binding was mon-itored by SPR|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2019/02/11	2020/02/18	-	rogid:voabMMncKJwjAiQ4kdUcGukdtUw10116	-	false	-	fc-igg tag:c-c	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
uniprotkb:Q62765	uniprotkb:Q62765	intact:EBI-7281118|intact:MINT-1526995|ensembl:ENSRNOP00000075793	intact:EBI-7281118|intact:MINT-1526995|ensembl:ENSRNOP00000075793	psi-mi:nlgn1_rat(display_long)|uniprotkb:Nlgn1(gene name)|psi-mi:Nlgn1(display_short)|uniprotkb:Neuroligin I(gene name synonym)	psi-mi:nlgn1_rat(display_long)|uniprotkb:Nlgn1(gene name)|psi-mi:Nlgn1(display_short)|uniprotkb:Neuroligin I(gene name synonym)	psi-mi:"MI:0028"(cosedimentation in solution)	Comoletti et al. (2003)	pubmed:14522992|imex:IM-26884	taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)")	taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)")	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0469"(IntAct)	intact:EBI-21226676|imex:IM-26884-3	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	rgd:621117|refseq:NP_446320.1|refseq:XP_017446075.1|refseq:XP_017446076.1|refseq:XP_017446077.1|refseq:XP_017446078.1|refseq:XP_017446079.1|refseq:XP_017446072.1|refseq:XP_017446073.1|refseq:XP_017446074.1|ensembl:ENSRNOG00000032576(gene)|ensembl:ENSRNOT00000092660(transcript)|go:"GO:0002087"(regulation of respiratory gaseous exchange by nervous system process)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005886"(plasma membrane)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0006605"(protein targeting)|go:"GO:0007157"(heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules)|go:"GO:0007158"(neuron cell-cell adhesion)|go:"GO:0007268"(chemical synaptic transmission)|go:"GO:0007399"(nervous system development)|go:"GO:0007416"(synapse assembly)|go:"GO:0009897"(external side of plasma membrane)|go:"GO:0009986"(cell surface)|go:"GO:0010841"(positive regulation of circadian sleep/wake cycle, wakefulness)|go:"GO:0014069"(postsynaptic density)|go:"GO:0016080"(synaptic vesicle targeting)|go:"GO:0016339"(calcium-dependent cell-cell adhesion via plasma membrane cell adhesion molecules)|go:"GO:0030165"(PDZ domain binding)|go:"GO:0030425"(dendrite)|go:"GO:0031175"(neuron projection development)|go:"GO:0032230"(positive regulation of synaptic transmission, GABAergic)|go:"GO:0032433"(filopodium tip)|go:"GO:0035418"(protein localization to synapse)|go:"GO:0038023"(signaling receptor activity)|go:"GO:0042043"(neurexin family protein binding)|go:"GO:0043083"(synaptic cleft)|go:"GO:0043197"(dendritic spine)|go:"GO:0043198"(dendritic shaft)|go:"GO:0043235"(receptor complex)|go:"GO:0044877"(protein-containing complex binding)|go:"GO:0045161"(neuronal ion channel clustering)|go:"GO:0045184"(establishment of protein localization)|go:"GO:0045202"(synapse)|go:"GO:0045664"(regulation of neuron differentiation)|go:"GO:0048488"(synaptic vesicle endocytosis)|go:"GO:0048489"(synaptic vesicle transport)|go:"GO:0042802"(identical protein binding)|go:"GO:0048511"(rhythmic process)|go:"GO:0048789"(cytoskeletal matrix organization at active zone)|go:"GO:0050804"(modulation of chemical synaptic transmission)|go:"GO:0050808"(synapse organization)|go:"GO:0050839"(cell adhesion molecule binding)|go:"GO:0051491"(positive regulation of filopodium assembly)|go:"GO:0051965"(positive regulation of synapse assembly)|go:"GO:0051968"(positive regulation of synaptic transmission, glutamatergic)|go:"GO:0060076"(excitatory synapse)|go:"GO:0060291"(long-term synaptic potentiation)|go:"GO:0060999"(positive regulation of dendritic spine development)|go:"GO:0061002"(negative regulation of dendritic spine morphogenesis)|go:"GO:0071277"(cellular response to calcium ion)|go:"GO:0072553"(terminal button organization)|go:"GO:0097091"(synaptic vesicle clustering)|go:"GO:0097104"(postsynaptic membrane assembly)|go:"GO:0097105"(presynaptic membrane assembly)|go:"GO:0097110"(scaffold protein binding)|go:"GO:0097113"(AMPA glutamate receptor clustering)|go:"GO:0097114"(NMDA glutamate receptor clustering)|go:"GO:0097115"(neurexin clustering involved in presynaptic membrane assembly)|go:"GO:0097119"(postsynaptic density protein 95 clustering)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098698"(postsynaptic specialization assembly)|go:"GO:0098793"(presynapse)|go:"GO:0098942"(retrograde trans-synaptic signaling by trans-synaptic protein complex)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099060"(integral component of postsynaptic specialization membrane)|go:"GO:0099174"(regulation of presynapse organization)|go:"GO:2000809"(positive regulation of synaptic vesicle clustering)|go:"GO:0099560"(synaptic membrane adhesion)|go:"GO:0140058"(neuron projection arborization)|interpro:IPR000460(Neuroligin)|interpro:IPR002018(Carboxylesterase, type B)|interpro:IPR019819|interpro:IPR029058|interpro:IPR030022|go:"GO:1900029"(positive regulation of ruffle assembly)|go:"GO:1900244"(positive regulation of synaptic vesicle endocytosis)|go:"GO:1902474"(positive regulation of protein localization to synapse)|go:"GO:1902533"(positive regulation of intracellular signal transduction)|go:"GO:1904861"(excitatory synapse assembly)|go:"GO:1905520"(positive regulation of presynaptic active zone assembly)|go:"GO:2000302"(positive regulation of synaptic vesicle exocytosis)|go:"GO:2000310"(regulation of NMDA receptor activity)|rcsb pdb:3BIW|go:"GO:2000311"(regulation of AMPA receptor activity)|go:"GO:2000463"(positive regulation of excitatory postsynaptic potential)|rcsb pdb:3BIX|rcsb pdb:3VKF|reactome:R-RNO-6794361|dip:DIP-44832N	rgd:621117|refseq:NP_446320.1|refseq:XP_017446075.1|refseq:XP_017446076.1|refseq:XP_017446077.1|refseq:XP_017446078.1|refseq:XP_017446079.1|refseq:XP_017446072.1|refseq:XP_017446073.1|refseq:XP_017446074.1|ensembl:ENSRNOG00000032576(gene)|ensembl:ENSRNOT00000092660(transcript)|go:"GO:0002087"(regulation of respiratory gaseous exchange by nervous system process)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005886"(plasma membrane)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0006605"(protein targeting)|go:"GO:0007157"(heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules)|go:"GO:0007158"(neuron cell-cell adhesion)|go:"GO:0007268"(chemical synaptic transmission)|go:"GO:0007399"(nervous system development)|go:"GO:0007416"(synapse assembly)|go:"GO:0009897"(external side of plasma membrane)|go:"GO:0009986"(cell surface)|go:"GO:0010841"(positive regulation of circadian sleep/wake cycle, wakefulness)|go:"GO:0014069"(postsynaptic density)|go:"GO:0016080"(synaptic vesicle targeting)|go:"GO:0016339"(calcium-dependent cell-cell adhesion via plasma membrane cell adhesion molecules)|go:"GO:0030165"(PDZ domain binding)|go:"GO:0030425"(dendrite)|go:"GO:0031175"(neuron projection development)|go:"GO:0032230"(positive regulation of synaptic transmission, GABAergic)|go:"GO:0032433"(filopodium tip)|go:"GO:0035418"(protein localization to synapse)|go:"GO:0038023"(signaling receptor activity)|go:"GO:0042043"(neurexin family protein binding)|go:"GO:0043083"(synaptic cleft)|go:"GO:0043197"(dendritic spine)|go:"GO:0043198"(dendritic shaft)|go:"GO:0043235"(receptor complex)|go:"GO:0044877"(protein-containing complex binding)|go:"GO:0045161"(neuronal ion channel clustering)|go:"GO:0045184"(establishment of protein localization)|go:"GO:0045202"(synapse)|go:"GO:0045664"(regulation of neuron differentiation)|go:"GO:0048488"(synaptic vesicle endocytosis)|go:"GO:0048489"(synaptic vesicle transport)|go:"GO:0042802"(identical protein binding)|go:"GO:0048511"(rhythmic process)|go:"GO:0048789"(cytoskeletal matrix organization at active zone)|go:"GO:0050804"(modulation of chemical synaptic transmission)|go:"GO:0050808"(synapse organization)|go:"GO:0050839"(cell adhesion molecule binding)|go:"GO:0051491"(positive regulation of filopodium assembly)|go:"GO:0051965"(positive regulation of synapse assembly)|go:"GO:0051968"(positive regulation of synaptic transmission, glutamatergic)|go:"GO:0060076"(excitatory synapse)|go:"GO:0060291"(long-term synaptic potentiation)|go:"GO:0060999"(positive regulation of dendritic spine development)|go:"GO:0061002"(negative regulation of dendritic spine morphogenesis)|go:"GO:0071277"(cellular response to calcium ion)|go:"GO:0072553"(terminal button organization)|go:"GO:0097091"(synaptic vesicle clustering)|go:"GO:0097104"(postsynaptic membrane assembly)|go:"GO:0097105"(presynaptic membrane assembly)|go:"GO:0097110"(scaffold protein binding)|go:"GO:0097113"(AMPA glutamate receptor clustering)|go:"GO:0097114"(NMDA glutamate receptor clustering)|go:"GO:0097115"(neurexin clustering involved in presynaptic membrane assembly)|go:"GO:0097119"(postsynaptic density protein 95 clustering)|go:"GO:0097120"(receptor localization to synapse)|go:"GO:0098698"(postsynaptic specialization assembly)|go:"GO:0098793"(presynapse)|go:"GO:0098942"(retrograde trans-synaptic signaling by trans-synaptic protein complex)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099060"(integral component of postsynaptic specialization membrane)|go:"GO:0099174"(regulation of presynapse organization)|go:"GO:2000809"(positive regulation of synaptic vesicle clustering)|go:"GO:0099560"(synaptic membrane adhesion)|go:"GO:0140058"(neuron projection arborization)|interpro:IPR000460(Neuroligin)|interpro:IPR002018(Carboxylesterase, type B)|interpro:IPR019819|interpro:IPR029058|interpro:IPR030022|go:"GO:1900029"(positive regulation of ruffle assembly)|go:"GO:1900244"(positive regulation of synaptic vesicle endocytosis)|go:"GO:1902474"(positive regulation of protein localization to synapse)|go:"GO:1902533"(positive regulation of intracellular signal transduction)|go:"GO:1904861"(excitatory synapse assembly)|go:"GO:1905520"(positive regulation of presynaptic active zone assembly)|go:"GO:2000302"(positive regulation of synaptic vesicle exocytosis)|go:"GO:2000310"(regulation of NMDA receptor activity)|rcsb pdb:3BIW|go:"GO:2000311"(regulation of AMPA receptor activity)|go:"GO:2000463"(positive regulation of excitatory postsynaptic potential)|rcsb pdb:3BIX|rcsb pdb:3VKF|reactome:R-RNO-6794361|dip:DIP-44832N	-	comment:mint|function:"Neuronal cell surface protein thought to be involved in cell-cell-interactions by forming intercellular junctions through binding to beta-neurexins. Seems to play role in formation or maintenance of synaptic junctions. In vitro, triggers the de novo formation of presynaptic structures (By similarity). May be involved in specification of excitatory synapses"	comment:mint|function:"Neuronal cell surface protein thought to be involved in cell-cell-interactions by forming intercellular junctions through binding to beta-neurexins. Seems to play role in formation or maintenance of synaptic junctions. In vitro, triggers the de novo formation of presynaptic structures (By similarity). May be involved in specification of excitatory synapses"	figure legend:Fig. 3|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2019/02/11	2019/03/01	rogid:I6UajYCOkz8f+3nt2IHH2rOCby810116	rogid:I6UajYCOkz8f+3nt2IHH2rOCby810116	rigid:0+QU25p3gIwwODkJxOHTOSS2pZw	false	flag tag:n-n|sufficient binding region:46-638	flag tag:n-n|sufficient binding region:46-638	2	0	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)