#ID(s) interactor A	ID(s) interactor B	Alt. ID(s) interactor A	Alt. ID(s) interactor B	Alias(es) interactor A	Alias(es) interactor B	Interaction detection method(s)	Publication 1st author(s)	Publication Identifier(s)	Taxid interactor A	Taxid interactor B	Interaction type(s)	Source database(s)	Interaction identifier(s)	Confidence value(s)	Expansion method(s)	Biological role(s) interactor A	Biological role(s) interactor B	Experimental role(s) interactor A	Experimental role(s) interactor B	Type(s) interactor A	Type(s) interactor B	Xref(s) interactor A	Xref(s) interactor B	Interaction Xref(s)	Annotation(s) interactor A	Annotation(s) interactor B	Interaction annotation(s)	Host organism(s)	Interaction parameter(s)	Creation date	Update date	Checksum(s) interactor A	Checksum(s) interactor B	Interaction Checksum(s)	Negative	Feature(s) interactor A	Feature(s) interactor B	Stoichiometry(s) interactor A	Stoichiometry(s) interactor B	Identification method participant A	Identification method participant B
uniprotkb:P0ADG1	uniprotkb:P0DP90	intact:EBI-1133722|uniprotkb:P13048|uniprotkb:P78269|uniprotkb:Q2M878|ensemblbacteria:AAC77489|ensemblbacteria:BAE77528	intact:EBI-1133701|uniprotkb:P00892|uniprotkb:P76749	psi-mi:ilvm_ecoli(display_long)|uniprotkb:Acetohydroxy-acid synthase II small subunit(gene name synonym)|uniprotkb:ALS-II(gene name synonym)|uniprotkb:ilvM(gene name)|psi-mi:ilvM(display_short)|uniprotkb:b3769(locus name)|uniprotkb:JW3742(locus name)	psi-mi:ilvg_ecoli(display_long)|uniprotkb:Acetohydroxy-acid synthase II large subunit(gene name synonym)|uniprotkb:ALS-II(gene name synonym)|uniprotkb:ilvG(gene name)|psi-mi:ilvG(display_short)	psi-mi:"MI:0096"(pull down)	Hill et al. (1997)	imex:IM-26470|pubmed:9581571	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0486"(UniProt)	intact:EBI-20586126|imex:IM-26470-1	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR002912(Amino acid-binding ACT)|refseq:WP_000983255.1|refseq:NP_418217.1|ensemblbacteria:AAC77489(transcript)|ensemblbacteria:b3769(gene)|ensemblbacteria:BAE77528(gene)|ensemblbacteria:BAE77528(transcript)|go:"GO:0003984"(acetolactate synthase activity)|go:"GO:0008652"(cellular amino acid biosynthetic process)|go:"GO:0009097"(isoleucine biosynthetic process)|go:"GO:0009099"(valine biosynthetic process)	interpro:IPR012846(Acetolactate synthase, large subunit, biosynthetic)|interpro:IPR012000(Thiamine pyrophosphate enzyme, central region)|interpro:IPR012001(Thiamine pyrophosphate enzyme, N-terminal TPP binding region)|interpro:IPR000399(TPP-binding enzymes, conserved site)|interpro:IPR011766(Thiamine pyrophosphate enzyme, C-terminal TPP-binding)|go:"GO:0000287"(magnesium ion binding)|go:"GO:0003984"(acetolactate synthase activity)|go:"GO:0009097"(isoleucine biosynthetic process)|go:"GO:0009099"(valine biosynthetic process)|go:"GO:0030976"(thiamine pyrophosphate binding)|go:"GO:0050660"(flavin adenine dinucleotide binding)|interpro:IPR029035|interpro:IPR029061|interpro:IPR039368	-	-	-	figure legend:Fig. 1, Table 1|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2018/06/08	2018/06/11	rogid:JlkMVZdRAwLi6NihnDzyUlJp4YM83333	rogid:zMNdGZgufTQROWys3vWNAtS0Vq483333	rigid:bxI6xbCBs3bVuIh/EXruo1CH6tQ	false	-	his tag:n-n	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0818"(molecular weight estimation by coomasie staining)|psi-mi:"MI:0039"(edman degradation)
uniprotkb:P0ADG1	uniprotkb:P0DP90	intact:EBI-1133722|uniprotkb:P13048|uniprotkb:P78269|uniprotkb:Q2M878|ensemblbacteria:AAC77489|ensemblbacteria:BAE77528	intact:EBI-1133701|uniprotkb:P00892|uniprotkb:P76749	psi-mi:ilvm_ecoli(display_long)|uniprotkb:Acetohydroxy-acid synthase II small subunit(gene name synonym)|uniprotkb:ALS-II(gene name synonym)|uniprotkb:ilvM(gene name)|psi-mi:ilvM(display_short)|uniprotkb:b3769(locus name)|uniprotkb:JW3742(locus name)	psi-mi:ilvg_ecoli(display_long)|uniprotkb:Acetohydroxy-acid synthase II large subunit(gene name synonym)|uniprotkb:ALS-II(gene name synonym)|uniprotkb:ilvG(gene name)|psi-mi:ilvG(display_short)	psi-mi:"MI:0096"(pull down)	Hill et al. (1997)	imex:IM-26470|pubmed:9581571	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0486"(UniProt)	intact:EBI-20586134|imex:IM-26470-3	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR002912(Amino acid-binding ACT)|refseq:WP_000983255.1|refseq:NP_418217.1|ensemblbacteria:AAC77489(transcript)|ensemblbacteria:b3769(gene)|ensemblbacteria:BAE77528(gene)|ensemblbacteria:BAE77528(transcript)|go:"GO:0003984"(acetolactate synthase activity)|go:"GO:0008652"(cellular amino acid biosynthetic process)|go:"GO:0009097"(isoleucine biosynthetic process)|go:"GO:0009099"(valine biosynthetic process)	interpro:IPR012846(Acetolactate synthase, large subunit, biosynthetic)|interpro:IPR012000(Thiamine pyrophosphate enzyme, central region)|interpro:IPR012001(Thiamine pyrophosphate enzyme, N-terminal TPP binding region)|interpro:IPR000399(TPP-binding enzymes, conserved site)|interpro:IPR011766(Thiamine pyrophosphate enzyme, C-terminal TPP-binding)|go:"GO:0000287"(magnesium ion binding)|go:"GO:0003984"(acetolactate synthase activity)|go:"GO:0009097"(isoleucine biosynthetic process)|go:"GO:0009099"(valine biosynthetic process)|go:"GO:0030976"(thiamine pyrophosphate binding)|go:"GO:0050660"(flavin adenine dinucleotide binding)|interpro:IPR029035|interpro:IPR029061|interpro:IPR039368	-	-	-	figure legend:Fig. 1, Table 2|comment:The native protein complex appeared to bind to the IMAC column via the small subunit|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2018/06/08	2018/06/11	rogid:JlkMVZdRAwLi6NihnDzyUlJp4YM83333	rogid:zMNdGZgufTQROWys3vWNAtS0Vq483333	rigid:bxI6xbCBs3bVuIh/EXruo1CH6tQ	false	-	-	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0818"(molecular weight estimation by coomasie staining)
chebi:"CHEBI:15361"	intenz:2.2.1.6	intact:EBI-6621808	intact:EBI-20586142	psi-mi:pyruvate(display_short)|psi-mi:"CHEBI:15361"(display_long)	psi-mi:ilvgm_ecoli(display_short)|psi-mi:2.2.1.6(display_long)|intact:"2xilvG:2xilvM"(complex systematic name)|intact:IlvGM complex(complex synonym)|intact:AHA II complex(complex synonym)|intact:Acetohydroxyacid synthase II complex(complex synonym)|intact:Acetolactate synthase II complex(complex recommended name)	psi-mi:"MI:0415"(enzymatic study)	Hill et al. (1997)	imex:IM-26470|pubmed:9581571	taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)")	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	psi-mi:"MI:0414"(enzymatic reaction)	psi-mi:"MI:0486"(UniProt)	intact:EBI-20586836|imex:IM-26470-4	-	-	psi-mi:"MI:0502"(enzyme target)	psi-mi:"MI:0501"(enzyme)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0328"(small molecule)	psi-mi:"MI:1302"(stable complex)	-	go:"GO:0009082"(branched-chain amino acid biosynthetic process)|go:"GO:0005948"(acetolactate synthase complex)|evidence ontology:"ECO:0000353"|go:"GO:0003984"(acetolactate synthase activity)|complex portal:CPX-3570(complex-primary)|intact:EBI-20586126(exp-evidence)|pubmed:9581571(see-also)	-	-	curated-complex:"Catalyzes the first step that is common to the biosynthesis of  branched-chain amino acids. The reaction involves the irreversible decarboxylation of pyruvate to a bound hydroxyethyl group that then condenses with either a second pyruvate molecule to form 2-acetolactate as the first step in the biosynthesis of valine and leucine or with 2-ketobutyrate to form 2-aceto-2-hydroxybutyrate as the initial  step in the biosynthesis of isoleucine.  The AHAS I (CPX-3573) and AHAS III (CPX-3575) complexes are both sensitive to feedback inhibition by valine, whereas the AHAS II complex is not, enabling bacteria expressing this complex to grow in valine-rich conditions."|complex-properties:FAD appears to be required to maintain the geometry of the active sites in the central beta‐domain of the ilvG molecule.|complex-assembly:Heterotetramer|comment:"ilvG is not commonly expressed in Escherichia coli K12 due to a frameshift that disturbs the ilvG reading frame, however, occasionally the ilv02096 mutation of a 2 bp insertion exists (as shown in PMID:9581571) which causes a frameshift that restores the open reading frame, permitting the expression of this protein."	figure legend:Fig. 2, 4 ,5|inhibition:Chlorimuron ethyl|inhibition:Chlorsulfuron|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2018/06/08	2018/06/14	-	-	-	false	-	-	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)