#ID(s) interactor A	ID(s) interactor B	Alt. ID(s) interactor A	Alt. ID(s) interactor B	Alias(es) interactor A	Alias(es) interactor B	Interaction detection method(s)	Publication 1st author(s)	Publication Identifier(s)	Taxid interactor A	Taxid interactor B	Interaction type(s)	Source database(s)	Interaction identifier(s)	Confidence value(s)	Expansion method(s)	Biological role(s) interactor A	Biological role(s) interactor B	Experimental role(s) interactor A	Experimental role(s) interactor B	Type(s) interactor A	Type(s) interactor B	Xref(s) interactor A	Xref(s) interactor B	Interaction Xref(s)	Annotation(s) interactor A	Annotation(s) interactor B	Interaction annotation(s)	Host organism(s)	Interaction parameter(s)	Creation date	Update date	Checksum(s) interactor A	Checksum(s) interactor B	Interaction Checksum(s)	Negative	Feature(s) interactor A	Feature(s) interactor B	Stoichiometry(s) interactor A	Stoichiometry(s) interactor B	Identification method participant A	Identification method participant B
uniprotkb:P08173	uniprotkb:P59768	intact:EBI-6655217|ensembl:ENSP00000409378|ensembl:ENSP00000507561|uniprotkb:B2RPP4|uniprotkb:Q0VD60|uniprotkb:Q4VBK7	intact:EBI-602681|uniprotkb:Q6P9A9|uniprotkb:Q5JPE2|ensembl:ENSP00000334448|ensembl:ENSP00000451102|ensembl:ENSP00000451231|ensembl:ENSP00000451576|ensembl:ENSP00000452014|ensembl:ENSP00000484021	psi-mi:acm4_human(display_long)|uniprotkb:CHRM4(gene name)|psi-mi:CHRM4(display_short)	psi-mi:gbg2_human(display_long)|uniprotkb:G gamma-I(gene name synonym)|uniprotkb:GNG2(gene name)|psi-mi:GNG2(display_short)	psi-mi:"MI:0410"(3D electron microscopy)	Draper-Joyce et al. (2018)	imex:IM-26776|pubmed:29925945	taxid:9606(human)|taxid:9606(Homo sapiens)	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0465"(DIP)	intact:EBI-20716467|wwpdb:6D9H|emdb:EMD-7835|imex:IM-26776-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	ensembl:ENSG00000180720(gene)|ensembl:ENST00000433765(transcript)|ensembl:ENST00000682254(transcript)|go:"GO:0004993"(G protein-coupled serotonin receptor activity)|go:"GO:0005886"(plasma membrane)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0030594"(neurotransmitter receptor activity)|go:"GO:0040012"(regulation of locomotion)|go:"GO:0045202"(synapse)|go:"GO:0045211"(postsynaptic membrane)|interpro:IPR000276(GPCR, rhodopsin-like)|interpro:IPR000995(Muscarinic acetylcholine receptor)|interpro:IPR001432(Muscarinic acetylcholine receptor M4)|interpro:IPR017452(GPCR, rhodopsin-like superfamily)|go:"GO:0007165"(signal transduction)|go:"GO:0007166"(cell surface receptor signaling pathway)|rcsb pdb:5DSG|rcsb pdb:6D9H|rcsb pdb:6KP6|reactome:R-HSA-390648|reactome:R-HSA-418594|go:"GO:0007187"(G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger)|go:"GO:0007197"(adenylate cyclase-inhibiting G protein-coupled acetylcholine receptor signaling pathway)|go:"GO:0007213"(G protein-coupled acetylcholine receptor signaling pathway)|go:"GO:0007268"(chemical synaptic transmission)|go:"GO:0016907"(G protein-coupled acetylcholine receptor activity)|go:"GO:0030425"(dendrite)|refseq:NP_000732.2|dip:DIP-61455N	refseq:NP_001230702.1|refseq:NP_001230703.1|refseq:NP_444292.1|refseq:XP_011535148.1|refseq:XP_016876865.1|refseq:XP_016876866.1|refseq:XP_006720236.1|dip:DIP-33949N|rcsb pdb:6OSA|rcsb pdb:6OT0|rcsb pdb:6P9X|rcsb pdb:6P9Y|rcsb pdb:6PB0|rcsb pdb:6PB1|rcsb pdb:6PT0|rcsb pdb:6UUN|rcsb pdb:6UUS|rcsb pdb:6UVA|rcsb pdb:6VCB|rcsb pdb:6VMS|rcsb pdb:6VN7|rcsb pdb:6WHA|rcsb pdb:6WHC|rcsb pdb:6WI9|rcsb pdb:6WPW|rcsb pdb:6WWZ|rcsb pdb:6WZG|rcsb pdb:6X18|rcsb pdb:6X19|rcsb pdb:6X1A|rcsb pdb:6XBJ|rcsb pdb:6XBK|rcsb pdb:6XBL|rcsb pdb:6XBM|rcsb pdb:6XOX|rcsb pdb:7AUE|rcsb pdb:7BB6|rcsb pdb:7BB7|rcsb pdb:7C2E|rcsb pdb:7CFM|rcsb pdb:7CFN|rcsb pdb:7CKW|rcsb pdb:7CKX|rcsb pdb:7CKY|rcsb pdb:7CKZ|rcsb pdb:7CMU|rcsb pdb:7CMV|rcsb pdb:7CRH|rcsb pdb:7CX2|rcsb pdb:7CX3|rcsb pdb:7CX4|rcsb pdb:7D76|rcsb pdb:7D77|rcsb pdb:7D7M|rcsb pdb:7DFL|rcsb pdb:7E2X|rcsb pdb:7E2Y|rcsb pdb:7E2Z|rcsb pdb:7E32|rcsb pdb:7E33|rcsb pdb:7E9G|rcsb pdb:7E9H|rcsb pdb:7EB2|rcsb pdb:7JOZ|rcsb pdb:7JV5|rcsb pdb:7JVP|rcsb pdb:7JVQ|rcsb pdb:7JVR|rcsb pdb:7K7L|rcsb pdb:7K7Z|rcsb pdb:7KH0|rcsb pdb:7L1U|rcsb pdb:7L1V|rcsb pdb:7LCI|rcsb pdb:7MBX|rcsb pdb:7MBY|reactome:R-HSA-1296041|reactome:R-HSA-163359|reactome:R-HSA-202040|reactome:R-HSA-381676|reactome:R-HSA-392170|reactome:R-HSA-392451|reactome:R-HSA-392851|reactome:R-HSA-400042|reactome:R-HSA-4086398|reactome:R-HSA-416476|reactome:R-HSA-416482|reactome:R-HSA-418217|reactome:R-HSA-418555|reactome:R-HSA-418592|reactome:R-HSA-418594|reactome:R-HSA-418597|reactome:R-HSA-420092|reactome:R-HSA-428930|reactome:R-HSA-432040|reactome:R-HSA-456926|reactome:R-HSA-500657|reactome:R-HSA-6814122|reactome:R-HSA-8964315|reactome:R-HSA-8964616|reactome:R-HSA-9009391|reactome:R-HSA-9660821|reactome:R-HSA-997272|ensembl:ENSG00000186469(gene)|ensembl:ENST00000335281(transcript)|ensembl:ENST00000554736(transcript)|ensembl:ENST00000555472(transcript)|ensembl:ENST00000556752(transcript)|ensembl:ENST00000556766(transcript)|ensembl:ENST00000615906(transcript)|go:"GO:0005834"(heterotrimeric G-protein complex)|go:"GO:0005886"(plasma membrane)|go:"GO:0007186"(G protein-coupled receptor signaling pathway)|rcsb pdb:6KPF|rcsb pdb:6KPG|rcsb pdb:6LFM|rcsb pdb:6LFO|rcsb pdb:6LI3|rcsb pdb:6LMK|rcsb pdb:6LML|rcsb pdb:6M1H|rcsb pdb:6M1I|rcsb pdb:6M8S|rcsb pdb:6N4B|rcsb pdb:6NI3|rcsb pdb:6NIY|rcsb pdb:6OIJ|rcsb pdb:6OIK|rcsb pdb:6OMM|rcsb pdb:6ORV|rcsb pdb:6K42|rcsb pdb:6OS9|go:"GO:0007191"(adenylate cyclase-activating dopamine receptor signaling pathway)|go:"GO:0016020"(membrane)|go:"GO:0031680"(G-protein beta/gamma-subunit complex)|go:"GO:0031681"(G-protein beta-subunit binding)|go:"GO:0070062"(extracellular exosome)|go:"GO:0071380"(cellular response to prostaglandin E stimulus)|go:"GO:0071870"(cellular response to catecholamine stimulus)|interpro:IPR001770(G-protein, gamma subunit)|interpro:IPR015898(G-protein, gamma-like subunit)|interpro:IPR036284|rcsb pdb:4KFM|rcsb pdb:5HE0|rcsb pdb:5HE1|rcsb pdb:5HE2|rcsb pdb:5HE3|rcsb pdb:5UKK|rcsb pdb:5UKL|rcsb pdb:5UKM|rcsb pdb:5UZ7|rcsb pdb:6B3J|rcsb pdb:6CRK|rcsb pdb:6D9H|rcsb pdb:6DDE|rcsb pdb:6DDF|rcsb pdb:6E3Y|rcsb pdb:6EG8|rcsb pdb:6G79|rcsb pdb:6GDG|rcsb pdb:6K41	-	-	-	figure legend:Fig 1., Extended Data Fig. 3, Extended Data Table 1|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2018/08/07	2018/08/07	rogid:Vl9zYuPmhxVM2yGmmttbZYJNDHM9606	rogid:8asTjDtSU6sCPSMwcXMbV340Rt49606	rigid:SppMPNaXLw/Jli5GDqlISe7Y2Ek	false	flag tag:n-n|his tag:c-c	-	1	1	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
uniprotkb:P08173	uniprotkb:P04899-1	intact:EBI-6655217|ensembl:ENSP00000409378|ensembl:ENSP00000507561|uniprotkb:B2RPP4|uniprotkb:Q0VD60|uniprotkb:Q4VBK7	intact:EBI-20716561	psi-mi:acm4_human(display_long)|uniprotkb:CHRM4(gene name)|psi-mi:CHRM4(display_short)	psi-mi:p04899-1(display_long)|uniprotkb:GNAI2(gene name)|psi-mi:GNAI2(display_short)|uniprotkb:GNAI2B(gene name synonym)	psi-mi:"MI:0410"(3D electron microscopy)	Draper-Joyce et al. (2018)	imex:IM-26776|pubmed:29925945	taxid:9606(human)|taxid:9606(Homo sapiens)	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0465"(DIP)	intact:EBI-20716467|wwpdb:6D9H|emdb:EMD-7835|imex:IM-26776-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	ensembl:ENSG00000180720(gene)|ensembl:ENST00000433765(transcript)|ensembl:ENST00000682254(transcript)|go:"GO:0004993"(G protein-coupled serotonin receptor activity)|go:"GO:0005886"(plasma membrane)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0030594"(neurotransmitter receptor activity)|go:"GO:0040012"(regulation of locomotion)|go:"GO:0045202"(synapse)|go:"GO:0045211"(postsynaptic membrane)|interpro:IPR000276(GPCR, rhodopsin-like)|interpro:IPR000995(Muscarinic acetylcholine receptor)|interpro:IPR001432(Muscarinic acetylcholine receptor M4)|interpro:IPR017452(GPCR, rhodopsin-like superfamily)|go:"GO:0007165"(signal transduction)|go:"GO:0007166"(cell surface receptor signaling pathway)|rcsb pdb:5DSG|rcsb pdb:6D9H|rcsb pdb:6KP6|reactome:R-HSA-390648|reactome:R-HSA-418594|go:"GO:0007187"(G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger)|go:"GO:0007197"(adenylate cyclase-inhibiting G protein-coupled acetylcholine receptor signaling pathway)|go:"GO:0007213"(G protein-coupled acetylcholine receptor signaling pathway)|go:"GO:0007268"(chemical synaptic transmission)|go:"GO:0016907"(G protein-coupled acetylcholine receptor activity)|go:"GO:0030425"(dendrite)|refseq:NP_000732.2|dip:DIP-61455N	uniprotkb:P04899-1(isoform-parent)	-	-	-	figure legend:Fig 1., Extended Data Fig. 3, Extended Data Table 1|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2018/08/07	2018/08/07	rogid:Vl9zYuPmhxVM2yGmmttbZYJNDHM9606	rogid:Mi3PAXudkdPb375bOi7luNAmUaU9606	rigid:SppMPNaXLw/Jli5GDqlISe7Y2Ek	false	flag tag:n-n|his tag:c-c	mutation:47-47,204-204,327-327,246-246	1	1	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
uniprotkb:P08173	uniprotkb:P62873-1	intact:EBI-6655217|ensembl:ENSP00000409378|ensembl:ENSP00000507561|uniprotkb:B2RPP4|uniprotkb:Q0VD60|uniprotkb:Q4VBK7	intact:EBI-20716555|ensembl:ENSP00000367872|ensembl:ENSP00000481878	psi-mi:acm4_human(display_long)|uniprotkb:CHRM4(gene name)|psi-mi:CHRM4(display_short)	psi-mi:p62873-1(display_long)|uniprotkb:GNB1(gene name)|psi-mi:GNB1(display_short)|uniprotkb:Transducin beta chain 1(gene name synonym)	psi-mi:"MI:0410"(3D electron microscopy)	Draper-Joyce et al. (2018)	imex:IM-26776|pubmed:29925945	taxid:9606(human)|taxid:9606(Homo sapiens)	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0465"(DIP)	intact:EBI-20716467|wwpdb:6D9H|emdb:EMD-7835|imex:IM-26776-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	ensembl:ENSG00000180720(gene)|ensembl:ENST00000433765(transcript)|ensembl:ENST00000682254(transcript)|go:"GO:0004993"(G protein-coupled serotonin receptor activity)|go:"GO:0005886"(plasma membrane)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0030594"(neurotransmitter receptor activity)|go:"GO:0040012"(regulation of locomotion)|go:"GO:0045202"(synapse)|go:"GO:0045211"(postsynaptic membrane)|interpro:IPR000276(GPCR, rhodopsin-like)|interpro:IPR000995(Muscarinic acetylcholine receptor)|interpro:IPR001432(Muscarinic acetylcholine receptor M4)|interpro:IPR017452(GPCR, rhodopsin-like superfamily)|go:"GO:0007165"(signal transduction)|go:"GO:0007166"(cell surface receptor signaling pathway)|rcsb pdb:5DSG|rcsb pdb:6D9H|rcsb pdb:6KP6|reactome:R-HSA-390648|reactome:R-HSA-418594|go:"GO:0007187"(G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger)|go:"GO:0007197"(adenylate cyclase-inhibiting G protein-coupled acetylcholine receptor signaling pathway)|go:"GO:0007213"(G protein-coupled acetylcholine receptor signaling pathway)|go:"GO:0007268"(chemical synaptic transmission)|go:"GO:0016907"(G protein-coupled acetylcholine receptor activity)|go:"GO:0030425"(dendrite)|refseq:NP_000732.2|dip:DIP-61455N	ensembl:ENST00000378609(transcript)|ensembl:ENST00000610897(transcript)|ensembl:ENSG00000078369(gene)|intact:EBI-357130(isoform-parent)	-	-	-	figure legend:Fig 1., Extended Data Fig. 3, Extended Data Table 1|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2018/08/07	2018/08/07	rogid:Vl9zYuPmhxVM2yGmmttbZYJNDHM9606	rogid:zP/G70MXiSQpzbyyWFTWrJFQENk9606	rigid:SppMPNaXLw/Jli5GDqlISe7Y2Ek	false	flag tag:n-n|his tag:c-c	-	1	1	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
uniprotkb:P08173	uniprotkb:P59768	intact:EBI-6655217|ensembl:ENSP00000409378|ensembl:ENSP00000507561|uniprotkb:B2RPP4|uniprotkb:Q0VD60|uniprotkb:Q4VBK7	intact:EBI-602681|uniprotkb:Q6P9A9|uniprotkb:Q5JPE2|ensembl:ENSP00000334448|ensembl:ENSP00000451102|ensembl:ENSP00000451231|ensembl:ENSP00000451576|ensembl:ENSP00000452014|ensembl:ENSP00000484021	psi-mi:acm4_human(display_long)|uniprotkb:CHRM4(gene name)|psi-mi:CHRM4(display_short)	psi-mi:gbg2_human(display_long)|uniprotkb:G gamma-I(gene name synonym)|uniprotkb:GNG2(gene name)|psi-mi:GNG2(display_short)	psi-mi:"MI:0071"(molecular sieving)	Draper-Joyce et al. (2018)	imex:IM-26776|pubmed:29925945	taxid:9606(human)|taxid:9606(Homo sapiens)	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0465"(DIP)	intact:EBI-20716622|imex:IM-26776-3	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	ensembl:ENSG00000180720(gene)|ensembl:ENST00000433765(transcript)|ensembl:ENST00000682254(transcript)|go:"GO:0004993"(G protein-coupled serotonin receptor activity)|go:"GO:0005886"(plasma membrane)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0030594"(neurotransmitter receptor activity)|go:"GO:0040012"(regulation of locomotion)|go:"GO:0045202"(synapse)|go:"GO:0045211"(postsynaptic membrane)|interpro:IPR000276(GPCR, rhodopsin-like)|interpro:IPR000995(Muscarinic acetylcholine receptor)|interpro:IPR001432(Muscarinic acetylcholine receptor M4)|interpro:IPR017452(GPCR, rhodopsin-like superfamily)|go:"GO:0007165"(signal transduction)|go:"GO:0007166"(cell surface receptor signaling pathway)|rcsb pdb:5DSG|rcsb pdb:6D9H|rcsb pdb:6KP6|reactome:R-HSA-390648|reactome:R-HSA-418594|go:"GO:0007187"(G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger)|go:"GO:0007197"(adenylate cyclase-inhibiting G protein-coupled acetylcholine receptor signaling pathway)|go:"GO:0007213"(G protein-coupled acetylcholine receptor signaling pathway)|go:"GO:0007268"(chemical synaptic transmission)|go:"GO:0016907"(G protein-coupled acetylcholine receptor activity)|go:"GO:0030425"(dendrite)|refseq:NP_000732.2|dip:DIP-61455N	refseq:NP_001230702.1|refseq:NP_001230703.1|refseq:NP_444292.1|refseq:XP_011535148.1|refseq:XP_016876865.1|refseq:XP_016876866.1|refseq:XP_006720236.1|dip:DIP-33949N|rcsb pdb:6OSA|rcsb pdb:6OT0|rcsb pdb:6P9X|rcsb pdb:6P9Y|rcsb pdb:6PB0|rcsb pdb:6PB1|rcsb pdb:6PT0|rcsb pdb:6UUN|rcsb pdb:6UUS|rcsb pdb:6UVA|rcsb pdb:6VCB|rcsb pdb:6VMS|rcsb pdb:6VN7|rcsb pdb:6WHA|rcsb pdb:6WHC|rcsb pdb:6WI9|rcsb pdb:6WPW|rcsb pdb:6WWZ|rcsb pdb:6WZG|rcsb pdb:6X18|rcsb pdb:6X19|rcsb pdb:6X1A|rcsb pdb:6XBJ|rcsb pdb:6XBK|rcsb pdb:6XBL|rcsb pdb:6XBM|rcsb pdb:6XOX|rcsb pdb:7AUE|rcsb pdb:7BB6|rcsb pdb:7BB7|rcsb pdb:7C2E|rcsb pdb:7CFM|rcsb pdb:7CFN|rcsb pdb:7CKW|rcsb pdb:7CKX|rcsb pdb:7CKY|rcsb pdb:7CKZ|rcsb pdb:7CMU|rcsb pdb:7CMV|rcsb pdb:7CRH|rcsb pdb:7CX2|rcsb pdb:7CX3|rcsb pdb:7CX4|rcsb pdb:7D76|rcsb pdb:7D77|rcsb pdb:7D7M|rcsb pdb:7DFL|rcsb pdb:7E2X|rcsb pdb:7E2Y|rcsb pdb:7E2Z|rcsb pdb:7E32|rcsb pdb:7E33|rcsb pdb:7E9G|rcsb pdb:7E9H|rcsb pdb:7EB2|rcsb pdb:7JOZ|rcsb pdb:7JV5|rcsb pdb:7JVP|rcsb pdb:7JVQ|rcsb pdb:7JVR|rcsb pdb:7K7L|rcsb pdb:7K7Z|rcsb pdb:7KH0|rcsb pdb:7L1U|rcsb pdb:7L1V|rcsb pdb:7LCI|rcsb pdb:7MBX|rcsb pdb:7MBY|reactome:R-HSA-1296041|reactome:R-HSA-163359|reactome:R-HSA-202040|reactome:R-HSA-381676|reactome:R-HSA-392170|reactome:R-HSA-392451|reactome:R-HSA-392851|reactome:R-HSA-400042|reactome:R-HSA-4086398|reactome:R-HSA-416476|reactome:R-HSA-416482|reactome:R-HSA-418217|reactome:R-HSA-418555|reactome:R-HSA-418592|reactome:R-HSA-418594|reactome:R-HSA-418597|reactome:R-HSA-420092|reactome:R-HSA-428930|reactome:R-HSA-432040|reactome:R-HSA-456926|reactome:R-HSA-500657|reactome:R-HSA-6814122|reactome:R-HSA-8964315|reactome:R-HSA-8964616|reactome:R-HSA-9009391|reactome:R-HSA-9660821|reactome:R-HSA-997272|ensembl:ENSG00000186469(gene)|ensembl:ENST00000335281(transcript)|ensembl:ENST00000554736(transcript)|ensembl:ENST00000555472(transcript)|ensembl:ENST00000556752(transcript)|ensembl:ENST00000556766(transcript)|ensembl:ENST00000615906(transcript)|go:"GO:0005834"(heterotrimeric G-protein complex)|go:"GO:0005886"(plasma membrane)|go:"GO:0007186"(G protein-coupled receptor signaling pathway)|rcsb pdb:6KPF|rcsb pdb:6KPG|rcsb pdb:6LFM|rcsb pdb:6LFO|rcsb pdb:6LI3|rcsb pdb:6LMK|rcsb pdb:6LML|rcsb pdb:6M1H|rcsb pdb:6M1I|rcsb pdb:6M8S|rcsb pdb:6N4B|rcsb pdb:6NI3|rcsb pdb:6NIY|rcsb pdb:6OIJ|rcsb pdb:6OIK|rcsb pdb:6OMM|rcsb pdb:6ORV|rcsb pdb:6K42|rcsb pdb:6OS9|go:"GO:0007191"(adenylate cyclase-activating dopamine receptor signaling pathway)|go:"GO:0016020"(membrane)|go:"GO:0031680"(G-protein beta/gamma-subunit complex)|go:"GO:0031681"(G-protein beta-subunit binding)|go:"GO:0070062"(extracellular exosome)|go:"GO:0071380"(cellular response to prostaglandin E stimulus)|go:"GO:0071870"(cellular response to catecholamine stimulus)|interpro:IPR001770(G-protein, gamma subunit)|interpro:IPR015898(G-protein, gamma-like subunit)|interpro:IPR036284|rcsb pdb:4KFM|rcsb pdb:5HE0|rcsb pdb:5HE1|rcsb pdb:5HE2|rcsb pdb:5HE3|rcsb pdb:5UKK|rcsb pdb:5UKL|rcsb pdb:5UKM|rcsb pdb:5UZ7|rcsb pdb:6B3J|rcsb pdb:6CRK|rcsb pdb:6D9H|rcsb pdb:6DDE|rcsb pdb:6DDF|rcsb pdb:6E3Y|rcsb pdb:6EG8|rcsb pdb:6G79|rcsb pdb:6GDG|rcsb pdb:6K41	-	-	-	figure legend:Fig 1., Extended Data Fig. 3, Extended Data Table 1|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2018/08/07	2018/08/07	rogid:Vl9zYuPmhxVM2yGmmttbZYJNDHM9606	rogid:8asTjDtSU6sCPSMwcXMbV340Rt49606	rigid:SppMPNaXLw/Jli5GDqlISe7Y2Ek	false	flag tag:n-n|his tag:c-c	-	1	1	psi-mi:"MI:0818"(molecular weight estimation by coomasie staining)	psi-mi:"MI:0818"(molecular weight estimation by coomasie staining)
uniprotkb:P08173	uniprotkb:P04899-1	intact:EBI-6655217|ensembl:ENSP00000409378|ensembl:ENSP00000507561|uniprotkb:B2RPP4|uniprotkb:Q0VD60|uniprotkb:Q4VBK7	intact:EBI-20716561	psi-mi:acm4_human(display_long)|uniprotkb:CHRM4(gene name)|psi-mi:CHRM4(display_short)	psi-mi:p04899-1(display_long)|uniprotkb:GNAI2(gene name)|psi-mi:GNAI2(display_short)|uniprotkb:GNAI2B(gene name synonym)	psi-mi:"MI:0071"(molecular sieving)	Draper-Joyce et al. (2018)	imex:IM-26776|pubmed:29925945	taxid:9606(human)|taxid:9606(Homo sapiens)	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0465"(DIP)	intact:EBI-20716622|imex:IM-26776-3	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	ensembl:ENSG00000180720(gene)|ensembl:ENST00000433765(transcript)|ensembl:ENST00000682254(transcript)|go:"GO:0004993"(G protein-coupled serotonin receptor activity)|go:"GO:0005886"(plasma membrane)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0030594"(neurotransmitter receptor activity)|go:"GO:0040012"(regulation of locomotion)|go:"GO:0045202"(synapse)|go:"GO:0045211"(postsynaptic membrane)|interpro:IPR000276(GPCR, rhodopsin-like)|interpro:IPR000995(Muscarinic acetylcholine receptor)|interpro:IPR001432(Muscarinic acetylcholine receptor M4)|interpro:IPR017452(GPCR, rhodopsin-like superfamily)|go:"GO:0007165"(signal transduction)|go:"GO:0007166"(cell surface receptor signaling pathway)|rcsb pdb:5DSG|rcsb pdb:6D9H|rcsb pdb:6KP6|reactome:R-HSA-390648|reactome:R-HSA-418594|go:"GO:0007187"(G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger)|go:"GO:0007197"(adenylate cyclase-inhibiting G protein-coupled acetylcholine receptor signaling pathway)|go:"GO:0007213"(G protein-coupled acetylcholine receptor signaling pathway)|go:"GO:0007268"(chemical synaptic transmission)|go:"GO:0016907"(G protein-coupled acetylcholine receptor activity)|go:"GO:0030425"(dendrite)|refseq:NP_000732.2|dip:DIP-61455N	uniprotkb:P04899-1(isoform-parent)	-	-	-	figure legend:Fig 1., Extended Data Fig. 3, Extended Data Table 1|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2018/08/07	2018/08/07	rogid:Vl9zYuPmhxVM2yGmmttbZYJNDHM9606	rogid:Mi3PAXudkdPb375bOi7luNAmUaU9606	rigid:SppMPNaXLw/Jli5GDqlISe7Y2Ek	false	flag tag:n-n|his tag:c-c	mutation:47-47,204-204,327-327,246-246	1	1	psi-mi:"MI:0818"(molecular weight estimation by coomasie staining)	psi-mi:"MI:0818"(molecular weight estimation by coomasie staining)
uniprotkb:P08173	uniprotkb:P62873-1	intact:EBI-6655217|ensembl:ENSP00000409378|ensembl:ENSP00000507561|uniprotkb:B2RPP4|uniprotkb:Q0VD60|uniprotkb:Q4VBK7	intact:EBI-20716555|ensembl:ENSP00000367872|ensembl:ENSP00000481878	psi-mi:acm4_human(display_long)|uniprotkb:CHRM4(gene name)|psi-mi:CHRM4(display_short)	psi-mi:p62873-1(display_long)|uniprotkb:GNB1(gene name)|psi-mi:GNB1(display_short)|uniprotkb:Transducin beta chain 1(gene name synonym)	psi-mi:"MI:0071"(molecular sieving)	Draper-Joyce et al. (2018)	imex:IM-26776|pubmed:29925945	taxid:9606(human)|taxid:9606(Homo sapiens)	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0465"(DIP)	intact:EBI-20716622|imex:IM-26776-3	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	ensembl:ENSG00000180720(gene)|ensembl:ENST00000433765(transcript)|ensembl:ENST00000682254(transcript)|go:"GO:0004993"(G protein-coupled serotonin receptor activity)|go:"GO:0005886"(plasma membrane)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0030594"(neurotransmitter receptor activity)|go:"GO:0040012"(regulation of locomotion)|go:"GO:0045202"(synapse)|go:"GO:0045211"(postsynaptic membrane)|interpro:IPR000276(GPCR, rhodopsin-like)|interpro:IPR000995(Muscarinic acetylcholine receptor)|interpro:IPR001432(Muscarinic acetylcholine receptor M4)|interpro:IPR017452(GPCR, rhodopsin-like superfamily)|go:"GO:0007165"(signal transduction)|go:"GO:0007166"(cell surface receptor signaling pathway)|rcsb pdb:5DSG|rcsb pdb:6D9H|rcsb pdb:6KP6|reactome:R-HSA-390648|reactome:R-HSA-418594|go:"GO:0007187"(G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger)|go:"GO:0007197"(adenylate cyclase-inhibiting G protein-coupled acetylcholine receptor signaling pathway)|go:"GO:0007213"(G protein-coupled acetylcholine receptor signaling pathway)|go:"GO:0007268"(chemical synaptic transmission)|go:"GO:0016907"(G protein-coupled acetylcholine receptor activity)|go:"GO:0030425"(dendrite)|refseq:NP_000732.2|dip:DIP-61455N	ensembl:ENST00000378609(transcript)|ensembl:ENST00000610897(transcript)|ensembl:ENSG00000078369(gene)|intact:EBI-357130(isoform-parent)	-	-	-	figure legend:Fig 1., Extended Data Fig. 3, Extended Data Table 1|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2018/08/07	2018/08/07	rogid:Vl9zYuPmhxVM2yGmmttbZYJNDHM9606	rogid:zP/G70MXiSQpzbyyWFTWrJFQENk9606	rigid:SppMPNaXLw/Jli5GDqlISe7Y2Ek	false	flag tag:n-n|his tag:c-c	-	1	1	psi-mi:"MI:0818"(molecular weight estimation by coomasie staining)	psi-mi:"MI:0818"(molecular weight estimation by coomasie staining)