#ID(s) interactor A	ID(s) interactor B	Alt. ID(s) interactor A	Alt. ID(s) interactor B	Alias(es) interactor A	Alias(es) interactor B	Interaction detection method(s)	Publication 1st author(s)	Publication Identifier(s)	Taxid interactor A	Taxid interactor B	Interaction type(s)	Source database(s)	Interaction identifier(s)	Confidence value(s)	Expansion method(s)	Biological role(s) interactor A	Biological role(s) interactor B	Experimental role(s) interactor A	Experimental role(s) interactor B	Type(s) interactor A	Type(s) interactor B	Xref(s) interactor A	Xref(s) interactor B	Interaction Xref(s)	Annotation(s) interactor A	Annotation(s) interactor B	Interaction annotation(s)	Host organism(s)	Interaction parameter(s)	Creation date	Update date	Checksum(s) interactor A	Checksum(s) interactor B	Interaction Checksum(s)	Negative	Feature(s) interactor A	Feature(s) interactor B	Stoichiometry(s) interactor A	Stoichiometry(s) interactor B	Identification method participant A	Identification method participant B
uniprotkb:Q15661-PRO_0000027480	uniprotkb:Q15661-PRO_0000027480	intact:EBI-20764158	intact:EBI-20764158	psi-mi:q15661-pro_0000027480(display_long)|uniprotkb:TPSAB1(gene name)|psi-mi:TPSAB1(display_short)|uniprotkb:TPS1(gene name synonym)|uniprotkb:TPS2(gene name synonym)|uniprotkb:TPSB1(gene name synonym)|uniprotkb:Tryptase I(gene name synonym)|uniprotkb:Tryptase alpha-1(gene name synonym)	psi-mi:q15661-pro_0000027480(display_long)|uniprotkb:TPSAB1(gene name)|psi-mi:TPSAB1(display_short)|uniprotkb:TPS1(gene name synonym)|uniprotkb:TPS2(gene name synonym)|uniprotkb:TPSB1(gene name synonym)|uniprotkb:Tryptase I(gene name synonym)|uniprotkb:Tryptase alpha-1(gene name synonym)	psi-mi:"MI:0114"(x-ray crystallography)	Maun et al. (2018)	pubmed:29661938|imex:IM-26650	taxid:9606(human)|taxid:9606(Homo sapiens)	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0469"(IntAct)	intact:EBI-20764120|wwpdb:5wi6|imex:IM-26650-1	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	intact:EBI-1761369(chain-parent)	intact:EBI-1761369(chain-parent)	-	chain-seq-start:31|chain-seq-end:275	chain-seq-start:31|chain-seq-end:275	figure legend:Table 3, Fig.5|3d-resolution:2.72A|3d-r-factors:Rworking 0.193 Rfree 0.227|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2018/09/19	2018/09/19	rogid:ndyr0G6hXCJqUSQfeX+Xfy3qHPI9606	rogid:ndyr0G6hXCJqUSQfeX+Xfy3qHPI9606	rigid:ppVaWEqDh2Hmi3EtUNCCtha9HQU	false	mutation:88-88	mutation:88-88	4	0	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
uniprotkb:Q15661	uniprotkb:Q15661	intact:EBI-1761369|intact:EBI-1762849|uniprotkb:D2E6R9|uniprotkb:D2E6S1|uniprotkb:P15157|uniprotkb:Q9H2Y5|uniprotkb:Q15663|uniprotkb:Q9H2Y4|uniprotkb:Q9UQI1|uniprotkb:Q6B052|ensembl:ENSP00000343577|ensembl:ENSP00000502948	intact:EBI-1761369|intact:EBI-1762849|uniprotkb:D2E6R9|uniprotkb:D2E6S1|uniprotkb:P15157|uniprotkb:Q9H2Y5|uniprotkb:Q15663|uniprotkb:Q9H2Y4|uniprotkb:Q9UQI1|uniprotkb:Q6B052|ensembl:ENSP00000343577|ensembl:ENSP00000502948	psi-mi:tryb1_human(display_long)|uniprotkb:TPSAB1(gene name)|psi-mi:TPSAB1(display_short)|uniprotkb:TPSB1(gene name synonym)|uniprotkb:Tryptase I(gene name synonym)|uniprotkb:TPS1(gene name synonym)|uniprotkb:TPS2(gene name synonym)|uniprotkb:Tryptase alpha-1(gene name synonym)	psi-mi:tryb1_human(display_long)|uniprotkb:TPSAB1(gene name)|psi-mi:TPSAB1(display_short)|uniprotkb:TPSB1(gene name synonym)|uniprotkb:Tryptase I(gene name synonym)|uniprotkb:TPS1(gene name synonym)|uniprotkb:TPS2(gene name synonym)|uniprotkb:Tryptase alpha-1(gene name synonym)	psi-mi:"MI:0071"(molecular sieving)	Maun et al. (2018)	pubmed:29661938|imex:IM-26650	taxid:9606(human)|taxid:9606(Homo sapiens)	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0469"(IntAct)	intact:EBI-20764149|imex:IM-26650-3	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_003285.2|ensembl:ENSG00000172236(gene)|ensembl:ENST00000338844(transcript)|ensembl:ENST00000677899(transcript)|go:"GO:0004252"(serine-type endopeptidase activity)|go:"GO:0005576"(extracellular region)|go:"GO:0005615"(extracellular space)|go:"GO:0006508"(proteolysis)|go:"GO:0006952"(defense response)|go:"GO:0008236"(serine-type peptidase activity)|go:"GO:0022617"(extracellular matrix disassembly)|go:"GO:0042802"(identical protein binding)|interpro:IPR001254(Peptidase S1 and S6, chymotrypsin/Hap)|interpro:IPR001314(Peptidase S1A, chymotrypsin)|interpro:IPR009003(Peptidase, trypsin-like serine and cysteine)|interpro:IPR018114|interpro:IPR033116|interpro:IPR043504|rcsb pdb:1LTO|rcsb pdb:2F9N|rcsb pdb:2F9O|rcsb pdb:2F9P|rcsb pdb:2ZEB|rcsb pdb:2ZEC|rcsb pdb:4A6L|rcsb pdb:4MPU|rcsb pdb:4MPV|rcsb pdb:4MPW|rcsb pdb:4MPX|rcsb pdb:4MQA|rcsb pdb:5F03|rcsb pdb:5WI6|rcsb pdb:6O1F|rcsb pdb:6P0P|rcsb pdb:6VVU|reactome:R-HSA-1592389	refseq:NP_003285.2|ensembl:ENSG00000172236(gene)|ensembl:ENST00000338844(transcript)|ensembl:ENST00000677899(transcript)|go:"GO:0004252"(serine-type endopeptidase activity)|go:"GO:0005576"(extracellular region)|go:"GO:0005615"(extracellular space)|go:"GO:0006508"(proteolysis)|go:"GO:0006952"(defense response)|go:"GO:0008236"(serine-type peptidase activity)|go:"GO:0022617"(extracellular matrix disassembly)|go:"GO:0042802"(identical protein binding)|interpro:IPR001254(Peptidase S1 and S6, chymotrypsin/Hap)|interpro:IPR001314(Peptidase S1A, chymotrypsin)|interpro:IPR009003(Peptidase, trypsin-like serine and cysteine)|interpro:IPR018114|interpro:IPR033116|interpro:IPR043504|rcsb pdb:1LTO|rcsb pdb:2F9N|rcsb pdb:2F9O|rcsb pdb:2F9P|rcsb pdb:2ZEB|rcsb pdb:2ZEC|rcsb pdb:4A6L|rcsb pdb:4MPU|rcsb pdb:4MPV|rcsb pdb:4MPW|rcsb pdb:4MPX|rcsb pdb:4MQA|rcsb pdb:5F03|rcsb pdb:5WI6|rcsb pdb:6O1F|rcsb pdb:6P0P|rcsb pdb:6VVU|reactome:R-HSA-1592389	-	-	-	figure legend:Fig.2, Fig.4A, Fig.8|comment:"As shown in Fig. 2, WT Beta-tryptase migrates as a tetramer. However, the I16G N-terminal insertion mutant does not, instead migrating as a monomer with a comparable retention time to the Beta-tryptase zymogen [...]  Serendipitously, we found monomeric fractions that were cysteinylated at I99C (I99C*), which prevents dimerization with another -tryptase monomer via the large interface, whereas the mutations Y75A and Y37bA abrogate interactions via the small interface, thus preventing tetramer formation."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2018/09/19	2018/09/19	rogid:I/QoDM5Sfej1YpvO9kbj9/wUwBI9606	rogid:I/QoDM5Sfej1YpvO9kbj9/wUwBI9606	rigid:zsgicAAr6hFWqAjRHQwJ1CPistQ	false	mutation increasing interaction strength:97-97|mutation increasing interaction strength:118-118|necessary binding region:31-275|mutation disrupting interaction strength:31-31|mutation disrupting interaction strength:97-97,118-118,54-54	mutation increasing interaction strength:97-97|mutation increasing interaction strength:118-118|necessary binding region:31-275|mutation disrupting interaction strength:31-31|mutation disrupting interaction strength:97-97,118-118,54-54	4	0	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
uniprotkb:Q15661	uniprotkb:Q15661	intact:EBI-1761369|intact:EBI-1762849|uniprotkb:D2E6R9|uniprotkb:D2E6S1|uniprotkb:P15157|uniprotkb:Q9H2Y5|uniprotkb:Q15663|uniprotkb:Q9H2Y4|uniprotkb:Q9UQI1|uniprotkb:Q6B052|ensembl:ENSP00000343577|ensembl:ENSP00000502948	intact:EBI-1761369|intact:EBI-1762849|uniprotkb:D2E6R9|uniprotkb:D2E6S1|uniprotkb:P15157|uniprotkb:Q9H2Y5|uniprotkb:Q15663|uniprotkb:Q9H2Y4|uniprotkb:Q9UQI1|uniprotkb:Q6B052|ensembl:ENSP00000343577|ensembl:ENSP00000502948	psi-mi:tryb1_human(display_long)|uniprotkb:TPSAB1(gene name)|psi-mi:TPSAB1(display_short)|uniprotkb:TPSB1(gene name synonym)|uniprotkb:Tryptase I(gene name synonym)|uniprotkb:TPS1(gene name synonym)|uniprotkb:TPS2(gene name synonym)|uniprotkb:Tryptase alpha-1(gene name synonym)	psi-mi:tryb1_human(display_long)|uniprotkb:TPSAB1(gene name)|psi-mi:TPSAB1(display_short)|uniprotkb:TPSB1(gene name synonym)|uniprotkb:Tryptase I(gene name synonym)|uniprotkb:TPS1(gene name synonym)|uniprotkb:TPS2(gene name synonym)|uniprotkb:Tryptase alpha-1(gene name synonym)	psi-mi:"MI:0071"(molecular sieving)	Maun et al. (2018)	pubmed:29661938|imex:IM-26650	taxid:9606(human)|taxid:9606(Homo sapiens)	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0469"(IntAct)	intact:EBI-20764190|imex:IM-26650-5	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_003285.2|ensembl:ENSG00000172236(gene)|ensembl:ENST00000338844(transcript)|ensembl:ENST00000677899(transcript)|go:"GO:0004252"(serine-type endopeptidase activity)|go:"GO:0005576"(extracellular region)|go:"GO:0005615"(extracellular space)|go:"GO:0006508"(proteolysis)|go:"GO:0006952"(defense response)|go:"GO:0008236"(serine-type peptidase activity)|go:"GO:0022617"(extracellular matrix disassembly)|go:"GO:0042802"(identical protein binding)|interpro:IPR001254(Peptidase S1 and S6, chymotrypsin/Hap)|interpro:IPR001314(Peptidase S1A, chymotrypsin)|interpro:IPR009003(Peptidase, trypsin-like serine and cysteine)|interpro:IPR018114|interpro:IPR033116|interpro:IPR043504|rcsb pdb:1LTO|rcsb pdb:2F9N|rcsb pdb:2F9O|rcsb pdb:2F9P|rcsb pdb:2ZEB|rcsb pdb:2ZEC|rcsb pdb:4A6L|rcsb pdb:4MPU|rcsb pdb:4MPV|rcsb pdb:4MPW|rcsb pdb:4MPX|rcsb pdb:4MQA|rcsb pdb:5F03|rcsb pdb:5WI6|rcsb pdb:6O1F|rcsb pdb:6P0P|rcsb pdb:6VVU|reactome:R-HSA-1592389	refseq:NP_003285.2|ensembl:ENSG00000172236(gene)|ensembl:ENST00000338844(transcript)|ensembl:ENST00000677899(transcript)|go:"GO:0004252"(serine-type endopeptidase activity)|go:"GO:0005576"(extracellular region)|go:"GO:0005615"(extracellular space)|go:"GO:0006508"(proteolysis)|go:"GO:0006952"(defense response)|go:"GO:0008236"(serine-type peptidase activity)|go:"GO:0022617"(extracellular matrix disassembly)|go:"GO:0042802"(identical protein binding)|interpro:IPR001254(Peptidase S1 and S6, chymotrypsin/Hap)|interpro:IPR001314(Peptidase S1A, chymotrypsin)|interpro:IPR009003(Peptidase, trypsin-like serine and cysteine)|interpro:IPR018114|interpro:IPR033116|interpro:IPR043504|rcsb pdb:1LTO|rcsb pdb:2F9N|rcsb pdb:2F9O|rcsb pdb:2F9P|rcsb pdb:2ZEB|rcsb pdb:2ZEC|rcsb pdb:4A6L|rcsb pdb:4MPU|rcsb pdb:4MPV|rcsb pdb:4MPW|rcsb pdb:4MPX|rcsb pdb:4MQA|rcsb pdb:5F03|rcsb pdb:5WI6|rcsb pdb:6O1F|rcsb pdb:6P0P|rcsb pdb:6VVU|reactome:R-HSA-1592389	-	-	-	figure legend:Fig.4A, Fig.8|comment:"we engineered a dimer at the large interface (I99C/Y75A/Y37bA) unable to form a tetramer based on SEC (Fig. 8)[...]Serendipitously, we found monomeric fractions that were cysteinylated at I99C (I99C*), which prevents dimerization with another -tryptase monomer via the large interface, whereas the mutations Y75A and Y37bA abrogate interactions via the small interface, thus preventing tetramer formation."|comment:heparin stabilises the dimer|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2018/09/19	2018/09/19	rogid:I/QoDM5Sfej1YpvO9kbj9/wUwBI9606	rogid:I/QoDM5Sfej1YpvO9kbj9/wUwBI9606	rigid:zsgicAAr6hFWqAjRHQwJ1CPistQ	false	mutation causing an interaction:97-97|mutation causing an interaction:118-118|necessary binding region:31-275|uncategorized protein modification:118-118|mutation causing an interaction:97-97,118-118,54-54	mutation causing an interaction:97-97|mutation causing an interaction:118-118|necessary binding region:31-275|uncategorized protein modification:118-118|mutation causing an interaction:97-97,118-118,54-54	2	0	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
uniprotkb:Q15661	uniprotkb:Q15661	intact:EBI-1761369|intact:EBI-1762849|uniprotkb:D2E6R9|uniprotkb:D2E6S1|uniprotkb:P15157|uniprotkb:Q9H2Y5|uniprotkb:Q15663|uniprotkb:Q9H2Y4|uniprotkb:Q9UQI1|uniprotkb:Q6B052|ensembl:ENSP00000343577|ensembl:ENSP00000502948	intact:EBI-1761369|intact:EBI-1762849|uniprotkb:D2E6R9|uniprotkb:D2E6S1|uniprotkb:P15157|uniprotkb:Q9H2Y5|uniprotkb:Q15663|uniprotkb:Q9H2Y4|uniprotkb:Q9UQI1|uniprotkb:Q6B052|ensembl:ENSP00000343577|ensembl:ENSP00000502948	psi-mi:tryb1_human(display_long)|uniprotkb:TPSAB1(gene name)|psi-mi:TPSAB1(display_short)|uniprotkb:TPSB1(gene name synonym)|uniprotkb:Tryptase I(gene name synonym)|uniprotkb:TPS1(gene name synonym)|uniprotkb:TPS2(gene name synonym)|uniprotkb:Tryptase alpha-1(gene name synonym)	psi-mi:tryb1_human(display_long)|uniprotkb:TPSAB1(gene name)|psi-mi:TPSAB1(display_short)|uniprotkb:TPSB1(gene name synonym)|uniprotkb:Tryptase I(gene name synonym)|uniprotkb:TPS1(gene name synonym)|uniprotkb:TPS2(gene name synonym)|uniprotkb:Tryptase alpha-1(gene name synonym)	psi-mi:"MI:0808"(comigration in sds page)	Maun et al. (2018)	pubmed:29661938|imex:IM-26650	taxid:9606(human)|taxid:9606(Homo sapiens)	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0408"(disulfide bond)	psi-mi:"MI:0469"(IntAct)	intact:EBI-20764213|imex:IM-26650-7	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_003285.2|ensembl:ENSG00000172236(gene)|ensembl:ENST00000338844(transcript)|ensembl:ENST00000677899(transcript)|go:"GO:0004252"(serine-type endopeptidase activity)|go:"GO:0005576"(extracellular region)|go:"GO:0005615"(extracellular space)|go:"GO:0006508"(proteolysis)|go:"GO:0006952"(defense response)|go:"GO:0008236"(serine-type peptidase activity)|go:"GO:0022617"(extracellular matrix disassembly)|go:"GO:0042802"(identical protein binding)|interpro:IPR001254(Peptidase S1 and S6, chymotrypsin/Hap)|interpro:IPR001314(Peptidase S1A, chymotrypsin)|interpro:IPR009003(Peptidase, trypsin-like serine and cysteine)|interpro:IPR018114|interpro:IPR033116|interpro:IPR043504|rcsb pdb:1LTO|rcsb pdb:2F9N|rcsb pdb:2F9O|rcsb pdb:2F9P|rcsb pdb:2ZEB|rcsb pdb:2ZEC|rcsb pdb:4A6L|rcsb pdb:4MPU|rcsb pdb:4MPV|rcsb pdb:4MPW|rcsb pdb:4MPX|rcsb pdb:4MQA|rcsb pdb:5F03|rcsb pdb:5WI6|rcsb pdb:6O1F|rcsb pdb:6P0P|rcsb pdb:6VVU|reactome:R-HSA-1592389	refseq:NP_003285.2|ensembl:ENSG00000172236(gene)|ensembl:ENST00000338844(transcript)|ensembl:ENST00000677899(transcript)|go:"GO:0004252"(serine-type endopeptidase activity)|go:"GO:0005576"(extracellular region)|go:"GO:0005615"(extracellular space)|go:"GO:0006508"(proteolysis)|go:"GO:0006952"(defense response)|go:"GO:0008236"(serine-type peptidase activity)|go:"GO:0022617"(extracellular matrix disassembly)|go:"GO:0042802"(identical protein binding)|interpro:IPR001254(Peptidase S1 and S6, chymotrypsin/Hap)|interpro:IPR001314(Peptidase S1A, chymotrypsin)|interpro:IPR009003(Peptidase, trypsin-like serine and cysteine)|interpro:IPR018114|interpro:IPR033116|interpro:IPR043504|rcsb pdb:1LTO|rcsb pdb:2F9N|rcsb pdb:2F9O|rcsb pdb:2F9P|rcsb pdb:2ZEB|rcsb pdb:2ZEC|rcsb pdb:4A6L|rcsb pdb:4MPU|rcsb pdb:4MPV|rcsb pdb:4MPW|rcsb pdb:4MPX|rcsb pdb:4MQA|rcsb pdb:5F03|rcsb pdb:5WI6|rcsb pdb:6O1F|rcsb pdb:6P0P|rcsb pdb:6VVU|reactome:R-HSA-1592389	-	-	-	figure legend:Fig.4B|comment:"Analysis of tetramers by SDS-PAGE under nonreducing conditions showed WT migrating as a monomer, whereas the Y75C and I99C mutants migrated as dimers (Fig. 4B). These dimers collapsed to the monomers when analyzed by SDS-PAGE under reducing conditions, consistent with an intermolecular disulfide bond between the two monomers"|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2018/09/19	2018/09/19	rogid:I/QoDM5Sfej1YpvO9kbj9/wUwBI9606	rogid:I/QoDM5Sfej1YpvO9kbj9/wUwBI9606	rigid:zsgicAAr6hFWqAjRHQwJ1CPistQ	false	mutation causing an interaction:97-97|mutation causing an interaction:118-118	mutation causing an interaction:97-97|mutation causing an interaction:118-118	2	0	psi-mi:"MI:0818"(molecular weight estimation by coomasie staining)	psi-mi:"MI:0818"(molecular weight estimation by coomasie staining)