#ID(s) interactor A	ID(s) interactor B	Alt. ID(s) interactor A	Alt. ID(s) interactor B	Alias(es) interactor A	Alias(es) interactor B	Interaction detection method(s)	Publication 1st author(s)	Publication Identifier(s)	Taxid interactor A	Taxid interactor B	Interaction type(s)	Source database(s)	Interaction identifier(s)	Confidence value(s)	Expansion method(s)	Biological role(s) interactor A	Biological role(s) interactor B	Experimental role(s) interactor A	Experimental role(s) interactor B	Type(s) interactor A	Type(s) interactor B	Xref(s) interactor A	Xref(s) interactor B	Interaction Xref(s)	Annotation(s) interactor A	Annotation(s) interactor B	Interaction annotation(s)	Host organism(s)	Interaction parameter(s)	Creation date	Update date	Checksum(s) interactor A	Checksum(s) interactor B	Interaction Checksum(s)	Negative	Feature(s) interactor A	Feature(s) interactor B	Stoichiometry(s) interactor A	Stoichiometry(s) interactor B	Identification method participant A	Identification method participant B
uniprotkb:P03407	uniprotkb:P08631-2	intact:EBI-7355020|intact:MINT-1206762	intact:EBI-9834454|ensembl:ENSP00000429848|ensembl:ENSP00000486627	psi-mi:nef_hv1a2(display_long)|uniprotkb:nef(gene name)|psi-mi:nef(display_short)|uniprotkb:Negative factor(gene name synonym)|uniprotkb:3'ORF(gene name synonym)	psi-mi:p08631-2(display_long)|uniprotkb:p59-HCK(isoform synonym)|uniprotkb:p59Hck(isoform synonym)|uniprotkb:HCK(gene name)|psi-mi:HCK(display_short)|uniprotkb:Hematopoietic cell kinase(gene name synonym)|uniprotkb:Hemopoietic cell kinase(gene name synonym)|uniprotkb:p59-HCK/p60-HCK(gene name synonym)|uniprotkb:p59Hck(gene name synonym)|uniprotkb:p61Hck(gene name synonym)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Briggs et al. (1997)	pubmed:9218412|doi:10.1074/jbc.272.29.17899|imex:IM-24813	taxid:11685(hv1a2)|taxid:11685("Human immunodeficiency virus type 1 group M subtype B (isolate ARV2/SF2)")	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0465"(DIP)	intact:EBI-16213060|imex:IM-24813-1|dip:DIP-77835E	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	go:"GO:0005525"(GTP binding)|go:"GO:0005576"(extracellular region)|go:"GO:0007171"(activation of transmembrane receptor protein tyrosine kinase activity)|go:"GO:0016020"(membrane)|go:"GO:0017124"(SH3 domain binding)|go:"GO:0020002"(host cell plasma membrane)|go:"GO:0039504"(suppression by virus of host adaptive immune response)|go:"GO:0039505"(suppression by virus of host antigen processing and presentation of peptide antigen via MHC class II)|go:"GO:0039521"(suppression by virus of host autophagy)|go:"GO:0044178"(host cell Golgi membrane)|go:"GO:0046776"(suppression by virus of host antigen processing and presentation of peptide antigen via MHC class I)|interpro:IPR001558(HIV negative factor Nef)|interpro:IPR027480|interpro:IPR027481|rcsb pdb:3RBB|rcsb pdb:3REA|rcsb pdb:3REB|rcsb pdb:4ORZ|rcsb pdb:4U1K|rcsb pdb:4U1L|rcsb pdb:4U1M|rcsb pdb:4U1N|rcsb pdb:4U5W|rcsb pdb:5XOV|rcsb pdb:6B72|rcsb pdb:7D7S|dip:DIP-29212N	uniprotkb:P08631-2(isoform-parent)|intact:EBI-346340(isoform-parent)|ensembl:ENSG00000101336(gene)|ensembl:ENST00000520553(transcript)|ensembl:ENST00000629881(transcript)	psi-mi:"MI:0465"(dip)	function:"Down-modulates surface MHC-I molecules. Internalized molecules are sequestred to the trans-Golgi network (TGN). Also decreases cell surface CD4 antigen by interacting with the Src family kinase LCK thereby inducing LCK-CD4 dissociation and by increasing clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Peroxisomal acyl-coenzyme A thioester hydrolase 1 (PTE1) seems to be involved in this process. May serve as a direct bridge between CD4 and the cellular endocytic machinery by interacting with beta-COP and adaptins. In consequence, surface MHC-I and CD4 are decreased and infected cells are masked for immune recognition by cytotoxic T lymphocytes. Decreasing the number of viral receptors prevents reinfection by more HIV particles"|function:"Bypasses host T-cell signaling by inducing a transcriptional program nearly identical to that of anti-CD3 cell activation. Interacts with the TCR-zeta chain to up-regulate the Fas ligand (FasL). Increasing surface FasL molecules and decreasing surface MHC-I molecules send attacking cytotoxic T-cell into apoptosis"|function:Protects the infected cell from apoptosis in order to keep it alive until the next virus generation is ready to strike. Inhibits the Fas and TNFR-mediated death signals by blocking MAP3K5. Interacts with p53 and decreases the half-life of the latter, protecting the infected cell against p53-mediated apoptosis. Inhibits the apoptotic signals regulated by the Bcl-2 family proteins through the formation of a Nef/PI3-kinase/PAK2 complex that induces phosphorylation of Bad|comment:mint|comment:homomint|function:Early protein that seems to play a role in optimizing the host cell environment for viral replication without causing cell death by apoptosis. Enhances virus infectivity and pathogenicity	isoform-comment:Initiator Met-1 is removed. Contains a N-myristoyl glycine at position 2. Contains a S-palmitoyl cysteine at position 3.	full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)")	-	2017/08/08	2017/08/10	rogid:5t7cTiE8lwJClrc3l7SQN9+TC6M11685	rogid:qSgfntp+jqdyi7qsc7u7UA7ylO09606	rigid:zsQuq1TOfWv9czV+l8PSdB9NtmU	false	mutation disrupting interaction strength:76-76,73-73	-	-	-	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)