#ID(s) interactor A	ID(s) interactor B	Alt. ID(s) interactor A	Alt. ID(s) interactor B	Alias(es) interactor A	Alias(es) interactor B	Interaction detection method(s)	Publication 1st author(s)	Publication Identifier(s)	Taxid interactor A	Taxid interactor B	Interaction type(s)	Source database(s)	Interaction identifier(s)	Confidence value(s)	Expansion method(s)	Biological role(s) interactor A	Biological role(s) interactor B	Experimental role(s) interactor A	Experimental role(s) interactor B	Type(s) interactor A	Type(s) interactor B	Xref(s) interactor A	Xref(s) interactor B	Interaction Xref(s)	Annotation(s) interactor A	Annotation(s) interactor B	Interaction annotation(s)	Host organism(s)	Interaction parameter(s)	Creation date	Update date	Checksum(s) interactor A	Checksum(s) interactor B	Interaction Checksum(s)	Negative	Feature(s) interactor A	Feature(s) interactor B	Stoichiometry(s) interactor A	Stoichiometry(s) interactor B	Identification method participant A	Identification method participant B
chebi:"CHEBI:41321"	uniprotkb:O15118-1	intact:EBI-13641298|chebi:"CHEBI:35377"|chebi:"CHEBI:41316"|chebi:"CHEBI:86594"	intact:EBI-13641283|ensembl:ENSP00000269228	psi-mi:cholesterol sulfate(display_short)|psi-mi:"CHEBI:41321"(display_long)|intact:CHOLEST-5-EN-3-YL HYDROGEN SULFATE(synonym)|intact:Cholest-5-en-3beta-ol sulfate(synonym)|intact:cholesterol sulfate(synonym)|intact:cholesteryl sulfate(synonym)|intact:cholest-5-en-3beta-yl hydrogen sulfate(iupac name)	psi-mi:o15118-1(display_long)|uniprotkb:Niemann-Pick C1 protein(gene name synonym)|uniprotkb:NPC1(gene name)|psi-mi:NPC1(display_short)	psi-mi:"MI:0114"(x-ray crystallography)	Li et al. (2016)	pubmed:27551080|imex:IM-25660	taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)")	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0469"(IntAct)	intact:EBI-13641271|imex:IM-25660-1|wwpdb:5kwy	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0328"(small molecule)	psi-mi:"MI:0326"(protein)	-	intact:EBI-2368710(isoform-parent)|ensembl:ENSG00000141458(gene)|ensembl:ENST00000269228(transcript)	-	-	-	figure legend:Supp Table 1|3d-resolution:2.4A|3d-r-factors:R work =0.194  R free = 0.249|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2017/02/02	2017/02/21	-	rogid:3kG4MGzeShC2D5FsPoZZm1lEGlk9606	-	false	-	his tag:c-c|sufficient binding region:374-620	2	2	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
chebi:"CHEBI:41321"	uniprotkb:P61916-1	intact:EBI-13641298|chebi:"CHEBI:35377"|chebi:"CHEBI:41316"|chebi:"CHEBI:86594"	intact:EBI-13641273|ensembl:ENSP00000451112	psi-mi:cholesterol sulfate(display_short)|psi-mi:"CHEBI:41321"(display_long)|intact:CHOLEST-5-EN-3-YL HYDROGEN SULFATE(synonym)|intact:Cholest-5-en-3beta-ol sulfate(synonym)|intact:cholesterol sulfate(synonym)|intact:cholesteryl sulfate(synonym)|intact:cholest-5-en-3beta-yl hydrogen sulfate(iupac name)	psi-mi:p61916-1(display_long)|uniprotkb:Human epididymis-specific protein 1(gene name synonym)|uniprotkb:Niemann-Pick disease type C2 protein(gene name synonym)|uniprotkb:Epididymal secretory protein E1(gene name synonym)|uniprotkb:NPC2(gene name)|psi-mi:NPC2(display_short)|uniprotkb:HE1(gene name synonym)	psi-mi:"MI:0114"(x-ray crystallography)	Li et al. (2016)	pubmed:27551080|imex:IM-25660	taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)")	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0469"(IntAct)	intact:EBI-13641271|imex:IM-25660-1|wwpdb:5kwy	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0328"(small molecule)	psi-mi:"MI:0326"(protein)	-	intact:EBI-2368946(isoform-parent)|ensembl:ENSG00000119655(gene)|ensembl:ENST00000555619(transcript)	-	-	-	figure legend:Supp Table 1|3d-resolution:2.4A|3d-r-factors:R work =0.194  R free = 0.249|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2017/02/02	2017/02/21	-	rogid:v1WSWKL+SaMlpcVh7Qhespo84Lg9606	-	false	-	his tag:c-c|sufficient binding region:20-151	2	2	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
chebi:"CHEBI:41321"	uniprotkb:O35604	intact:EBI-13641298|chebi:"CHEBI:35377"|chebi:"CHEBI:41316"|chebi:"CHEBI:86594"	intact:EBI-13641434|ensembl:ENSMUSP00000025279|uniprotkb:G3X8W9|uniprotkb:O35605	psi-mi:cholesterol sulfate(display_short)|psi-mi:"CHEBI:41321"(display_long)|intact:CHOLEST-5-EN-3-YL HYDROGEN SULFATE(synonym)|intact:Cholest-5-en-3beta-ol sulfate(synonym)|intact:cholesterol sulfate(synonym)|intact:cholesteryl sulfate(synonym)|intact:cholest-5-en-3beta-yl hydrogen sulfate(iupac name)	psi-mi:npc1_mouse(display_long)|uniprotkb:Niemann-Pick C1 protein(gene name synonym)|uniprotkb:Npc1(gene name)|psi-mi:Npc1(display_short)	psi-mi:"MI:1247"(microscale thermophoresis)	Li et al. (2016)	pubmed:27551080|imex:IM-25660	taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)")	taxid:10090(mouse)|taxid:10090(Mus musculus)	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0469"(IntAct)	intact:EBI-13641313|imex:IM-25660-2	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0328"(small molecule)	psi-mi:"MI:0326"(protein)	-	ensembl:ENSMUSG00000024413(gene)|ensembl:ENSMUST00000025279(transcript)|go:"GO:0005319"(lipid transporter activity)|go:"GO:0005576"(extracellular region)|go:"GO:0005635"(nuclear envelope)|go:"GO:0005764"(lysosome)|go:"GO:0005768"(endosome)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005886"(plasma membrane)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0006897"(endocytosis)|go:"GO:0006914"(autophagy)|go:"GO:0007041"(lysosomal transport)|go:"GO:0007628"(adult walking behavior)|go:"GO:0008203"(cholesterol metabolic process)|go:"GO:0008206"(bile acid metabolic process)|go:"GO:0010467"(gene expression)|go:"GO:0015485"(cholesterol binding)|go:"GO:0016020"(membrane)|go:"GO:0016242"(negative regulation of macroautophagy)|go:"GO:0030301"(cholesterol transport)|go:"GO:0031579"(membrane raft organization)|go:"GO:0031902"(late endosome membrane)|go:"GO:0031982"(vesicle)|go:"GO:0032367"(intracellular cholesterol transport)|go:"GO:0033344"(cholesterol efflux)|go:"GO:0042493"|go:"GO:0042632"(cholesterol homeostasis)|go:"GO:0045121"(membrane raft)|go:"GO:0046686"(response to cadmium ion)|go:"GO:0046718"(viral entry into host cell)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0060548"(negative regulation of cell death)|go:"GO:0071383"(cellular response to steroid hormone stimulus)|go:"GO:0071404"(cellular response to low-density lipoprotein particle stimulus)|go:"GO:0090150"(establishment of protein localization to membrane)|go:"GO:1905103"(integral component of lysosomal membrane)|interpro:IPR000731(Sterol-sensing 5TM box)|interpro:IPR003392(Patched)|interpro:IPR004765(Niemann-Pick C type protein)|interpro:IPR032190|reactome:R-MMU-8964038|go:"GO:0006486"(protein glycosylation)|refseq:NP_032746.2	-	-	-	figure legend:Fig 3|3d-resolution:2.4A|comment:Both the D502A,F503A,Y504A and  E421F503A,Y504A  mutant constructs  gave a Kd of >3uM.|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	kd:429.0x10^-9(molar)	2017/02/02	2017/02/02	-	rogid:CO34Jav+6wUxOKDGoV+JTdYediM10090	-	false	-	tag:n-n|tag:c-c|mutation decreasing interaction strength:503-504|mutation decreasing interaction strength:502-504|mutation decreasing interaction strength:421-421,503-504|flag tag:n-n|his tag:n-n|sufficient binding region:371-621	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
chebi:"CHEBI:41321"	uniprotkb:P79345	intact:EBI-13641298|chebi:"CHEBI:35377"|chebi:"CHEBI:41316"|chebi:"CHEBI:86594"	intact:EBI-13641408|ensembl:ENSBTAP00000029271|uniprotkb:Q3T091|uniprotkb:Q58DR4	psi-mi:cholesterol sulfate(display_short)|psi-mi:"CHEBI:41321"(display_long)|intact:CHOLEST-5-EN-3-YL HYDROGEN SULFATE(synonym)|intact:Cholest-5-en-3beta-ol sulfate(synonym)|intact:cholesterol sulfate(synonym)|intact:cholesteryl sulfate(synonym)|intact:cholest-5-en-3beta-yl hydrogen sulfate(iupac name)	psi-mi:npc2_bovin(display_long)|uniprotkb:Epididymal secretory protein E1(gene name synonym)|uniprotkb:NPC2(gene name)|psi-mi:NPC2(display_short)|uniprotkb:Niemann Pick type C2 protein homolog(gene name synonym)|uniprotkb:EPV20(gene name synonym)	psi-mi:"MI:1247"(microscale thermophoresis)	Li et al. (2016)	pubmed:27551080|imex:IM-25660	taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)")	taxid:9913(bovin)|taxid:9913("Bos taurus (Bovine)")	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0469"(IntAct)	intact:EBI-13641313|imex:IM-25660-2	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0328"(small molecule)	psi-mi:"MI:0326"(protein)	-	ensembl:ENSBTAG00000021955(gene)|ensembl:ENSBTAT00000029271(transcript)|go:"GO:0005615"(extracellular space)|go:"GO:0005764"(lysosome)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0008203"(cholesterol metabolic process)|go:"GO:0009615"(response to virus)|go:"GO:0015485"(cholesterol binding)|go:"GO:0015918"(sterol transport)|go:"GO:0019899"(enzyme binding)|go:"GO:0030301"(cholesterol transport)|go:"GO:0032367"(intracellular cholesterol transport)|go:"GO:0032934"(sterol binding)|go:"GO:0033344"(cholesterol efflux)|go:"GO:0042632"(cholesterol homeostasis)|go:"GO:0120020"(cholesterol transfer activity)|interpro:IPR003172(MD-2-related lipid-recognition)|interpro:IPR014756(Immunoglobulin E-set)|interpro:IPR033916|interpro:IPR039670|rcsb pdb:1NEP|rcsb pdb:2HKA|reactome:R-BTA-6798695|reactome:R-BTA-8964038|refseq:NP_776343.1	-	-	-	figure legend:Fig 3|3d-resolution:2.4A|comment:Both the D502A,F503A,Y504A and  E421F503A,Y504A  mutant constructs  gave a Kd of >3uM.|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	kd:429.0x10^-9(molar)	2017/02/02	2017/02/02	-	rogid:vaTFg500kD8OWs3FEV86JDFAqnU9913	-	false	-	red fluorescent protein tag:?-?	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)