#ID(s) interactor A	ID(s) interactor B	Alt. ID(s) interactor A	Alt. ID(s) interactor B	Alias(es) interactor A	Alias(es) interactor B	Interaction detection method(s)	Publication 1st author(s)	Publication Identifier(s)	Taxid interactor A	Taxid interactor B	Interaction type(s)	Source database(s)	Interaction identifier(s)	Confidence value(s)	Expansion method(s)	Biological role(s) interactor A	Biological role(s) interactor B	Experimental role(s) interactor A	Experimental role(s) interactor B	Type(s) interactor A	Type(s) interactor B	Xref(s) interactor A	Xref(s) interactor B	Interaction Xref(s)	Annotation(s) interactor A	Annotation(s) interactor B	Interaction annotation(s)	Host organism(s)	Interaction parameter(s)	Creation date	Update date	Checksum(s) interactor A	Checksum(s) interactor B	Interaction Checksum(s)	Negative	Feature(s) interactor A	Feature(s) interactor B	Stoichiometry(s) interactor A	Stoichiometry(s) interactor B	Identification method participant A	Identification method participant B
uniprotkb:P0DPI0	uniprotkb:Q496J9	intact:EBI-8178893|uniprotkb:P18639|intact:MINT-1795451|uniprotkb:P01561|uniprotkb:A5HZZ9|uniprotkb:A7G1U9|uniprotkb:P10845	intact:EBI-16081469|intact:EBI-28984041|ensembl:ENSP00000423541|uniprotkb:Q496K1|uniprotkb:Q9UPU8	psi-mi:bxa1_clobo(display_long)|uniprotkb:botA(gene name)|psi-mi:botA(display_short)|uniprotkb:atx(gene name synonym)|uniprotkb:Bontoxilysin-A(gene name synonym)|uniprotkb:Botulinum neurotoxin type A1(gene name synonym)|uniprotkb:bonT(gene name synonym)	psi-mi:sv2c_human(display_long)|uniprotkb:SV2C(gene name)|psi-mi:SV2C(display_short)|uniprotkb:KIAA1054(gene name synonym)	psi-mi:"MI:0114"(x-ray crystallography)	Benoit et al. (2014)	pubmed:24240280|doi:10.1038/nature12732|imex:IM-22354	taxid:1491(clobo)|taxid:1491(Clostridium botulinum)	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0465"(DIP)	intact:EBI-16081503|imex:IM-22354-1|dip:DIP-195895E	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	rcsb pdb:4JRA|rcsb pdb:5JLV|rcsb pdb:5JMC|rcsb pdb:6XCB|rcsb pdb:6XCC|rcsb pdb:6XCE|rcsb pdb:6XCF|rcsb pdb:6DKK|rcsb pdb:6MHJ|rcsb pdb:2ISG|rcsb pdb:2IMC|rcsb pdb:2ILP|rcsb pdb:2IMA|rcsb pdb:2VUA|rcsb pdb:1UEE|rcsb pdb:4HEV|rcsb pdb:3V0C|rcsb pdb:2ISH|rcsb pdb:1XTF|rcsb pdb:2VU9|rcsb pdb:3ZUS|rcsb pdb:3BOO|rcsb pdb:1XTG|rcsb pdb:2ISE|rcsb pdb:2IMB|rcsb pdb:3BOK|rcsb pdb:2W2D|rcsb pdb:3V0B|rcsb pdb:3V0A|rcsb pdb:3BON|rcsb pdb:3ZUR|rcsb pdb:4IQP|rcsb pdb:5MK6|rcsb pdb:5MK7|rcsb pdb:5TPB|rcsb pdb:5TPC|rcsb pdb:5VGV|rcsb pdb:5VGX|rcsb pdb:5V8P|rcsb pdb:5V8R|rcsb pdb:5V8U|interpro:IPR000395(Peptidase M27, bontoxilysin)|interpro:IPR011065(Kunitz inhibitor ST1-like)|interpro:IPR012500(Clostridium neurotoxin, translocation)|interpro:IPR012928(Clostridium neurotoxin, receptor binding N-terminal)|interpro:IPR013104(Clostridium neurotoxin, receptor-binding C-terminal)|interpro:IPR013320(Concanavalin A-like lectin/glucanase, subgroup)|interpro:IPR036248|refseq:WP_003356619.1|refseq:WP_011948511.1|reactome:R-HSA-5250968|rcsb pdb:6XCD|go:"GO:0004222"(metalloendopeptidase activity)|go:"GO:0005576"(extracellular region)|go:"GO:0005618"(cell wall)|go:"GO:0008270"(zinc ion binding)|go:"GO:0008320"(protein transmembrane transporter activity)|go:"GO:0016021"(integral component of membrane)|go:"GO:0044156"(host cell junction)|go:"GO:0044161"(host cell cytoplasmic vesicle)|go:"GO:0044164"(host cell cytosol)|go:"GO:0044231"(host cell presynaptic membrane)|go:"GO:0046929"(negative regulation of neurotransmitter secretion)|go:"GO:0090729"(toxin activity)|go:"GO:1905576"(ganglioside GT1b binding)	ensembl:ENSP00000423541|ensembl:ENSG00000122012(gene)|ensembl:ENST00000502798(transcript)|go:"GO:0005886"(plasma membrane)|go:"GO:0006836"(neurotransmitter transport)|go:"GO:0007268"(chemical synaptic transmission)|go:"GO:0008021"(synaptic vesicle)|go:"GO:0016021"(integral component of membrane)|go:"GO:0022857"(transmembrane transporter activity)|go:"GO:0030285"(integral component of synaptic vesicle membrane)|go:"GO:0030672"(synaptic vesicle membrane)|go:"GO:0043005"(neuron projection)|interpro:IPR001646(Pentapeptide repeat)|interpro:IPR005828(General substrate transporter)|interpro:IPR005829(Sugar transporter, conserved site)|interpro:IPR011701(Major facilitator superfamily MFS-1)|interpro:IPR020846|interpro:IPR022308|interpro:IPR036259|rcsb pdb:4JRA|rcsb pdb:5JLV|rcsb pdb:5MOY|rcsb pdb:6ES1|reactome:R-HSA-5250955|reactome:R-HSA-5250968|reactome:R-HSA-5250981|refseq:NP_055794.3|refseq:XP_011541583.1|refseq:XP_011541584.2|dip:DIP-60687N	psi-mi:"MI:0465"(dip)	comment:mint|function:"Inhibits acetylcholine release. The botulinum toxin binds with high affinity to peripheral neuronal presynaptic membrane to the secretory vesicle protein SV2. It binds directly to the largest luminal loop of SV2A, SV2B and SV2C. It is then internalized by receptor-mediated endocytosis. The C-terminus of the heavy chain (H) is responsible for the adherence of the toxin to the cell surface while the N-terminus mediates transport of the light chain from the endocytic vesicle to the cytosol. After translocation, the light chain (L) hydrolyzes the 197-Gln-|-Arg-198 bond in SNAP-25, thereby blocking neurotransmitter release. Inhibition of acetylcholine release results in flaccid paralysis, with frequent heart or respiratory failure"	-	full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2017/08/07	2017/08/10	rogid:15Vc5B8nPpFHrwDzDcAWGGZaryk1491	rogid:ETD+5WxItqT0sxuqbQJzqhAPtF89606	rigid:JeZpKGX5O9xYo16ZmgtYY9nNojU	false	sufficient binding region:871-1296	sufficient binding region:456-574	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
uniprotkb:P0DPI0	uniprotkb:Q496J9	intact:EBI-8178893|uniprotkb:P18639|intact:MINT-1795451|uniprotkb:P01561|uniprotkb:A5HZZ9|uniprotkb:A7G1U9|uniprotkb:P10845	intact:EBI-16081469|intact:EBI-28984041|ensembl:ENSP00000423541|uniprotkb:Q496K1|uniprotkb:Q9UPU8	psi-mi:bxa1_clobo(display_long)|uniprotkb:botA(gene name)|psi-mi:botA(display_short)|uniprotkb:atx(gene name synonym)|uniprotkb:Bontoxilysin-A(gene name synonym)|uniprotkb:Botulinum neurotoxin type A1(gene name synonym)|uniprotkb:bonT(gene name synonym)	psi-mi:sv2c_human(display_long)|uniprotkb:SV2C(gene name)|psi-mi:SV2C(display_short)|uniprotkb:KIAA1054(gene name synonym)	psi-mi:"MI:0096"(pull down)	Benoit et al. (2014)	pubmed:24240280|doi:10.1038/nature12732|imex:IM-22354	taxid:1491(clobo)|taxid:1491(Clostridium botulinum)	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0465"(DIP)	intact:EBI-16081550|imex:IM-22354-3|dip:DIP-195895E	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	rcsb pdb:4JRA|rcsb pdb:5JLV|rcsb pdb:5JMC|rcsb pdb:6XCB|rcsb pdb:6XCC|rcsb pdb:6XCE|rcsb pdb:6XCF|rcsb pdb:6DKK|rcsb pdb:6MHJ|rcsb pdb:2ISG|rcsb pdb:2IMC|rcsb pdb:2ILP|rcsb pdb:2IMA|rcsb pdb:2VUA|rcsb pdb:1UEE|rcsb pdb:4HEV|rcsb pdb:3V0C|rcsb pdb:2ISH|rcsb pdb:1XTF|rcsb pdb:2VU9|rcsb pdb:3ZUS|rcsb pdb:3BOO|rcsb pdb:1XTG|rcsb pdb:2ISE|rcsb pdb:2IMB|rcsb pdb:3BOK|rcsb pdb:2W2D|rcsb pdb:3V0B|rcsb pdb:3V0A|rcsb pdb:3BON|rcsb pdb:3ZUR|rcsb pdb:4IQP|rcsb pdb:5MK6|rcsb pdb:5MK7|rcsb pdb:5TPB|rcsb pdb:5TPC|rcsb pdb:5VGV|rcsb pdb:5VGX|rcsb pdb:5V8P|rcsb pdb:5V8R|rcsb pdb:5V8U|interpro:IPR000395(Peptidase M27, bontoxilysin)|interpro:IPR011065(Kunitz inhibitor ST1-like)|interpro:IPR012500(Clostridium neurotoxin, translocation)|interpro:IPR012928(Clostridium neurotoxin, receptor binding N-terminal)|interpro:IPR013104(Clostridium neurotoxin, receptor-binding C-terminal)|interpro:IPR013320(Concanavalin A-like lectin/glucanase, subgroup)|interpro:IPR036248|refseq:WP_003356619.1|refseq:WP_011948511.1|reactome:R-HSA-5250968|rcsb pdb:6XCD|go:"GO:0004222"(metalloendopeptidase activity)|go:"GO:0005576"(extracellular region)|go:"GO:0005618"(cell wall)|go:"GO:0008270"(zinc ion binding)|go:"GO:0008320"(protein transmembrane transporter activity)|go:"GO:0016021"(integral component of membrane)|go:"GO:0044156"(host cell junction)|go:"GO:0044161"(host cell cytoplasmic vesicle)|go:"GO:0044164"(host cell cytosol)|go:"GO:0044231"(host cell presynaptic membrane)|go:"GO:0046929"(negative regulation of neurotransmitter secretion)|go:"GO:0090729"(toxin activity)|go:"GO:1905576"(ganglioside GT1b binding)	ensembl:ENSP00000423541|ensembl:ENSG00000122012(gene)|ensembl:ENST00000502798(transcript)|go:"GO:0005886"(plasma membrane)|go:"GO:0006836"(neurotransmitter transport)|go:"GO:0007268"(chemical synaptic transmission)|go:"GO:0008021"(synaptic vesicle)|go:"GO:0016021"(integral component of membrane)|go:"GO:0022857"(transmembrane transporter activity)|go:"GO:0030285"(integral component of synaptic vesicle membrane)|go:"GO:0030672"(synaptic vesicle membrane)|go:"GO:0043005"(neuron projection)|interpro:IPR001646(Pentapeptide repeat)|interpro:IPR005828(General substrate transporter)|interpro:IPR005829(Sugar transporter, conserved site)|interpro:IPR011701(Major facilitator superfamily MFS-1)|interpro:IPR020846|interpro:IPR022308|interpro:IPR036259|rcsb pdb:4JRA|rcsb pdb:5JLV|rcsb pdb:5MOY|rcsb pdb:6ES1|reactome:R-HSA-5250955|reactome:R-HSA-5250968|reactome:R-HSA-5250981|refseq:NP_055794.3|refseq:XP_011541583.1|refseq:XP_011541584.2|dip:DIP-60687N	psi-mi:"MI:0465"(dip)	comment:mint|function:"Inhibits acetylcholine release. The botulinum toxin binds with high affinity to peripheral neuronal presynaptic membrane to the secretory vesicle protein SV2. It binds directly to the largest luminal loop of SV2A, SV2B and SV2C. It is then internalized by receptor-mediated endocytosis. The C-terminus of the heavy chain (H) is responsible for the adherence of the toxin to the cell surface while the N-terminus mediates transport of the light chain from the endocytic vesicle to the cytosol. After translocation, the light chain (L) hydrolyzes the 197-Gln-|-Arg-198 bond in SNAP-25, thereby blocking neurotransmitter release. Inhibition of acetylcholine release results in flaccid paralysis, with frequent heart or respiratory failure"	-	full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2017/08/07	2017/08/10	rogid:15Vc5B8nPpFHrwDzDcAWGGZaryk1491	rogid:ETD+5WxItqT0sxuqbQJzqhAPtF89606	rigid:JeZpKGX5O9xYo16ZmgtYY9nNojU	false	sufficient binding region:871-1296|mutation decreasing interaction strength:1294-1294|mutation decreasing interaction strength:1156-1156|mutation disrupting interaction strength:1146-1146,1145-1145|his tag:n-n	sufficient binding region:456-574	-	-	psi-mi:"MI:0818"(molecular weight estimation by coomasie staining)	psi-mi:"MI:0818"(molecular weight estimation by coomasie staining)
uniprotkb:P0DPI0	uniprotkb:Q496J9	intact:EBI-8178893|uniprotkb:P18639|intact:MINT-1795451|uniprotkb:P01561|uniprotkb:A5HZZ9|uniprotkb:A7G1U9|uniprotkb:P10845	intact:EBI-16081469|intact:EBI-28984041|ensembl:ENSP00000423541|uniprotkb:Q496K1|uniprotkb:Q9UPU8	psi-mi:bxa1_clobo(display_long)|uniprotkb:botA(gene name)|psi-mi:botA(display_short)|uniprotkb:atx(gene name synonym)|uniprotkb:Bontoxilysin-A(gene name synonym)|uniprotkb:Botulinum neurotoxin type A1(gene name synonym)|uniprotkb:bonT(gene name synonym)	psi-mi:sv2c_human(display_long)|uniprotkb:SV2C(gene name)|psi-mi:SV2C(display_short)|uniprotkb:KIAA1054(gene name synonym)	psi-mi:"MI:0096"(pull down)	Benoit et al. (2014)	pubmed:24240280|doi:10.1038/nature12732|imex:IM-22354	taxid:1491(clobo)|taxid:1491(Clostridium botulinum)	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0465"(DIP)	intact:EBI-16081524|imex:IM-22354-2|dip:DIP-195895E	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	rcsb pdb:4JRA|rcsb pdb:5JLV|rcsb pdb:5JMC|rcsb pdb:6XCB|rcsb pdb:6XCC|rcsb pdb:6XCE|rcsb pdb:6XCF|rcsb pdb:6DKK|rcsb pdb:6MHJ|rcsb pdb:2ISG|rcsb pdb:2IMC|rcsb pdb:2ILP|rcsb pdb:2IMA|rcsb pdb:2VUA|rcsb pdb:1UEE|rcsb pdb:4HEV|rcsb pdb:3V0C|rcsb pdb:2ISH|rcsb pdb:1XTF|rcsb pdb:2VU9|rcsb pdb:3ZUS|rcsb pdb:3BOO|rcsb pdb:1XTG|rcsb pdb:2ISE|rcsb pdb:2IMB|rcsb pdb:3BOK|rcsb pdb:2W2D|rcsb pdb:3V0B|rcsb pdb:3V0A|rcsb pdb:3BON|rcsb pdb:3ZUR|rcsb pdb:4IQP|rcsb pdb:5MK6|rcsb pdb:5MK7|rcsb pdb:5TPB|rcsb pdb:5TPC|rcsb pdb:5VGV|rcsb pdb:5VGX|rcsb pdb:5V8P|rcsb pdb:5V8R|rcsb pdb:5V8U|interpro:IPR000395(Peptidase M27, bontoxilysin)|interpro:IPR011065(Kunitz inhibitor ST1-like)|interpro:IPR012500(Clostridium neurotoxin, translocation)|interpro:IPR012928(Clostridium neurotoxin, receptor binding N-terminal)|interpro:IPR013104(Clostridium neurotoxin, receptor-binding C-terminal)|interpro:IPR013320(Concanavalin A-like lectin/glucanase, subgroup)|interpro:IPR036248|refseq:WP_003356619.1|refseq:WP_011948511.1|reactome:R-HSA-5250968|rcsb pdb:6XCD|go:"GO:0004222"(metalloendopeptidase activity)|go:"GO:0005576"(extracellular region)|go:"GO:0005618"(cell wall)|go:"GO:0008270"(zinc ion binding)|go:"GO:0008320"(protein transmembrane transporter activity)|go:"GO:0016021"(integral component of membrane)|go:"GO:0044156"(host cell junction)|go:"GO:0044161"(host cell cytoplasmic vesicle)|go:"GO:0044164"(host cell cytosol)|go:"GO:0044231"(host cell presynaptic membrane)|go:"GO:0046929"(negative regulation of neurotransmitter secretion)|go:"GO:0090729"(toxin activity)|go:"GO:1905576"(ganglioside GT1b binding)	ensembl:ENSP00000423541|ensembl:ENSG00000122012(gene)|ensembl:ENST00000502798(transcript)|go:"GO:0005886"(plasma membrane)|go:"GO:0006836"(neurotransmitter transport)|go:"GO:0007268"(chemical synaptic transmission)|go:"GO:0008021"(synaptic vesicle)|go:"GO:0016021"(integral component of membrane)|go:"GO:0022857"(transmembrane transporter activity)|go:"GO:0030285"(integral component of synaptic vesicle membrane)|go:"GO:0030672"(synaptic vesicle membrane)|go:"GO:0043005"(neuron projection)|interpro:IPR001646(Pentapeptide repeat)|interpro:IPR005828(General substrate transporter)|interpro:IPR005829(Sugar transporter, conserved site)|interpro:IPR011701(Major facilitator superfamily MFS-1)|interpro:IPR020846|interpro:IPR022308|interpro:IPR036259|rcsb pdb:4JRA|rcsb pdb:5JLV|rcsb pdb:5MOY|rcsb pdb:6ES1|reactome:R-HSA-5250955|reactome:R-HSA-5250968|reactome:R-HSA-5250981|refseq:NP_055794.3|refseq:XP_011541583.1|refseq:XP_011541584.2|dip:DIP-60687N	psi-mi:"MI:0465"(dip)	comment:mint|function:"Inhibits acetylcholine release. The botulinum toxin binds with high affinity to peripheral neuronal presynaptic membrane to the secretory vesicle protein SV2. It binds directly to the largest luminal loop of SV2A, SV2B and SV2C. It is then internalized by receptor-mediated endocytosis. The C-terminus of the heavy chain (H) is responsible for the adherence of the toxin to the cell surface while the N-terminus mediates transport of the light chain from the endocytic vesicle to the cytosol. After translocation, the light chain (L) hydrolyzes the 197-Gln-|-Arg-198 bond in SNAP-25, thereby blocking neurotransmitter release. Inhibition of acetylcholine release results in flaccid paralysis, with frequent heart or respiratory failure"	-	full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2017/08/07	2017/08/10	rogid:15Vc5B8nPpFHrwDzDcAWGGZaryk1491	rogid:ETD+5WxItqT0sxuqbQJzqhAPtF89606	rigid:JeZpKGX5O9xYo16ZmgtYY9nNojU	false	his tag:n-n|sufficient binding region:871-1296	sufficient binding region:456-574|mutation disrupting interaction strength:563-563	-	-	psi-mi:"MI:0818"(molecular weight estimation by coomasie staining)	psi-mi:"MI:0818"(molecular weight estimation by coomasie staining)
uniprotkb:P0DPI0	uniprotkb:Q496J9	intact:EBI-8178893|uniprotkb:P18639|intact:MINT-1795451|uniprotkb:P01561|uniprotkb:A5HZZ9|uniprotkb:A7G1U9|uniprotkb:P10845	intact:EBI-16081469|intact:EBI-28984041|ensembl:ENSP00000423541|uniprotkb:Q496K1|uniprotkb:Q9UPU8	psi-mi:bxa1_clobo(display_long)|uniprotkb:botA(gene name)|psi-mi:botA(display_short)|uniprotkb:atx(gene name synonym)|uniprotkb:Bontoxilysin-A(gene name synonym)|uniprotkb:Botulinum neurotoxin type A1(gene name synonym)|uniprotkb:bonT(gene name synonym)	psi-mi:sv2c_human(display_long)|uniprotkb:SV2C(gene name)|psi-mi:SV2C(display_short)|uniprotkb:KIAA1054(gene name synonym)	psi-mi:"MI:0017"(classical fluorescence spectroscopy)	Benoit et al. (2014)	pubmed:24240280|doi:10.1038/nature12732|imex:IM-22354	taxid:1491(clobo)|taxid:1491(Clostridium botulinum)	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0465"(DIP)	intact:EBI-16081583|imex:IM-22354-4|dip:DIP-195895E	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	rcsb pdb:4JRA|rcsb pdb:5JLV|rcsb pdb:5JMC|rcsb pdb:6XCB|rcsb pdb:6XCC|rcsb pdb:6XCE|rcsb pdb:6XCF|rcsb pdb:6DKK|rcsb pdb:6MHJ|rcsb pdb:2ISG|rcsb pdb:2IMC|rcsb pdb:2ILP|rcsb pdb:2IMA|rcsb pdb:2VUA|rcsb pdb:1UEE|rcsb pdb:4HEV|rcsb pdb:3V0C|rcsb pdb:2ISH|rcsb pdb:1XTF|rcsb pdb:2VU9|rcsb pdb:3ZUS|rcsb pdb:3BOO|rcsb pdb:1XTG|rcsb pdb:2ISE|rcsb pdb:2IMB|rcsb pdb:3BOK|rcsb pdb:2W2D|rcsb pdb:3V0B|rcsb pdb:3V0A|rcsb pdb:3BON|rcsb pdb:3ZUR|rcsb pdb:4IQP|rcsb pdb:5MK6|rcsb pdb:5MK7|rcsb pdb:5TPB|rcsb pdb:5TPC|rcsb pdb:5VGV|rcsb pdb:5VGX|rcsb pdb:5V8P|rcsb pdb:5V8R|rcsb pdb:5V8U|interpro:IPR000395(Peptidase M27, bontoxilysin)|interpro:IPR011065(Kunitz inhibitor ST1-like)|interpro:IPR012500(Clostridium neurotoxin, translocation)|interpro:IPR012928(Clostridium neurotoxin, receptor binding N-terminal)|interpro:IPR013104(Clostridium neurotoxin, receptor-binding C-terminal)|interpro:IPR013320(Concanavalin A-like lectin/glucanase, subgroup)|interpro:IPR036248|refseq:WP_003356619.1|refseq:WP_011948511.1|reactome:R-HSA-5250968|rcsb pdb:6XCD|go:"GO:0004222"(metalloendopeptidase activity)|go:"GO:0005576"(extracellular region)|go:"GO:0005618"(cell wall)|go:"GO:0008270"(zinc ion binding)|go:"GO:0008320"(protein transmembrane transporter activity)|go:"GO:0016021"(integral component of membrane)|go:"GO:0044156"(host cell junction)|go:"GO:0044161"(host cell cytoplasmic vesicle)|go:"GO:0044164"(host cell cytosol)|go:"GO:0044231"(host cell presynaptic membrane)|go:"GO:0046929"(negative regulation of neurotransmitter secretion)|go:"GO:0090729"(toxin activity)|go:"GO:1905576"(ganglioside GT1b binding)	ensembl:ENSP00000423541|ensembl:ENSG00000122012(gene)|ensembl:ENST00000502798(transcript)|go:"GO:0005886"(plasma membrane)|go:"GO:0006836"(neurotransmitter transport)|go:"GO:0007268"(chemical synaptic transmission)|go:"GO:0008021"(synaptic vesicle)|go:"GO:0016021"(integral component of membrane)|go:"GO:0022857"(transmembrane transporter activity)|go:"GO:0030285"(integral component of synaptic vesicle membrane)|go:"GO:0030672"(synaptic vesicle membrane)|go:"GO:0043005"(neuron projection)|interpro:IPR001646(Pentapeptide repeat)|interpro:IPR005828(General substrate transporter)|interpro:IPR005829(Sugar transporter, conserved site)|interpro:IPR011701(Major facilitator superfamily MFS-1)|interpro:IPR020846|interpro:IPR022308|interpro:IPR036259|rcsb pdb:4JRA|rcsb pdb:5JLV|rcsb pdb:5MOY|rcsb pdb:6ES1|reactome:R-HSA-5250955|reactome:R-HSA-5250968|reactome:R-HSA-5250981|refseq:NP_055794.3|refseq:XP_011541583.1|refseq:XP_011541584.2|dip:DIP-60687N	psi-mi:"MI:0465"(dip)	comment:mint|function:"Inhibits acetylcholine release. The botulinum toxin binds with high affinity to peripheral neuronal presynaptic membrane to the secretory vesicle protein SV2. It binds directly to the largest luminal loop of SV2A, SV2B and SV2C. It is then internalized by receptor-mediated endocytosis. The C-terminus of the heavy chain (H) is responsible for the adherence of the toxin to the cell surface while the N-terminus mediates transport of the light chain from the endocytic vesicle to the cytosol. After translocation, the light chain (L) hydrolyzes the 197-Gln-|-Arg-198 bond in SNAP-25, thereby blocking neurotransmitter release. Inhibition of acetylcholine release results in flaccid paralysis, with frequent heart or respiratory failure"	-	full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2017/08/07	2017/08/10	rogid:15Vc5B8nPpFHrwDzDcAWGGZaryk1491	rogid:ETD+5WxItqT0sxuqbQJzqhAPtF89606	rigid:JeZpKGX5O9xYo16ZmgtYY9nNojU	false	sufficient binding region:871-1296|mutation decreasing interaction strength:1294-1294|mutation decreasing interaction strength:1156-1156|mutation decreasing interaction strength:1146-1146,1145-1145	sufficient binding region:456-574|fluorescein-5-maleimide label:?-?	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)