#ID(s) interactor A	ID(s) interactor B	Alt. ID(s) interactor A	Alt. ID(s) interactor B	Alias(es) interactor A	Alias(es) interactor B	Interaction detection method(s)	Publication 1st author(s)	Publication Identifier(s)	Taxid interactor A	Taxid interactor B	Interaction type(s)	Source database(s)	Interaction identifier(s)	Confidence value(s)	Expansion method(s)	Biological role(s) interactor A	Biological role(s) interactor B	Experimental role(s) interactor A	Experimental role(s) interactor B	Type(s) interactor A	Type(s) interactor B	Xref(s) interactor A	Xref(s) interactor B	Interaction Xref(s)	Annotation(s) interactor A	Annotation(s) interactor B	Interaction annotation(s)	Host organism(s)	Interaction parameter(s)	Creation date	Update date	Checksum(s) interactor A	Checksum(s) interactor B	Interaction Checksum(s)	Negative	Feature(s) interactor A	Feature(s) interactor B	Stoichiometry(s) interactor A	Stoichiometry(s) interactor B	Identification method participant A	Identification method participant B
uniprotkb:P0A892	uniprotkb:P0A6B9	intact:EBI-15849930|ensemblbacteria:AAG58579|ensemblbacteria:BAB37742|uniprotkb:P37618	intact:EBI-9011195|uniprotkb:P39171|uniprotkb:P76581|uniprotkb:P76992|uniprotkb:Q8XA86|ensemblbacteria:AAG57644|ensemblbacteria:BAB36819	psi-mi:tusa_eco57(display_long)|uniprotkb:tusA(gene name)|psi-mi:tusA(display_short)|uniprotkb:Sulfur mediator TusA(gene name synonym)|uniprotkb:Sulfur transfer protein TusA(gene name synonym)|uniprotkb:tRNA 2-thiouridine synthesizing protein A(gene name synonym)|uniprotkb:ECs4319(locus name)|uniprotkb:Z4844(locus name)	psi-mi:iscs_eco57(display_long)|uniprotkb:iscS(gene name)|psi-mi:iscS(display_short)|uniprotkb:ECs3396(locus name)|uniprotkb:Z3797(locus name)	psi-mi:"MI:0826"(x ray scattering)	Shi et al. (2010)	pubmed:20404999|doi:10.1371/journal.pbio.1000354|imex:IM-15204	taxid:83334(eco57)|taxid:83334("Escherichia coli O157:H7")	taxid:83334(eco57)|taxid:83334("Escherichia coli O157:H7")	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0465"(DIP)	intact:EBI-15849951|imex:IM-15204-1|dip:DIP-185636E	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	ensemblbacteria:AAG58579(transcript)|ensemblbacteria:BAB37742(transcript)|ensemblbacteria:ECs_4319(gene)|ensemblbacteria:Z4844(gene)|go:"GO:0002143"(tRNA wobble position uridine thiolation)|go:"GO:0097163"(sulfur carrier activity)|interpro:IPR001455(SirA-like)|interpro:IPR022931|interpro:IPR036868|rcsb pdb:3LVJ|rcsb pdb:3LVK|go:"GO:0005737"(cytoplasm)|refseq:NP_312346.1|refseq:WP_000130621.1|dip:DIP-58574N	refseq:NP_311423.2|dip:DIP-58573N|refseq:WP_001295373.1|ensemblbacteria:AAG57644(transcript)|ensemblbacteria:BAB36819(transcript)|ensemblbacteria:ECs_3396(gene)|ensemblbacteria:Z3797(gene)|go:"GO:0005737"(cytoplasm)|go:"GO:0008033"(tRNA processing)|go:"GO:0030170"(pyridoxal phosphate binding)|go:"GO:0031071"(cysteine desulfurase activity)|go:"GO:0044571"([2Fe-2S] cluster assembly)|go:"GO:0046872"(metal ion binding)|go:"GO:0051537"(2 iron, 2 sulfur cluster binding)|interpro:IPR000192(Aminotransferase, class V/Cysteine desulphurase)|interpro:IPR010240(Cysteine desulphurase)|interpro:IPR015421(Pyridoxal phosphate-dependent transferase, major region, subdomain 1)|interpro:IPR015422(Pyridoxal phosphate-dependent transferase, major region, subdomain 2)|interpro:IPR015424(Pyridoxal phosphate-dependent transferase, major region)|interpro:IPR016454(Cysteine desulphurase, NifS)|interpro:IPR020578|rcsb pdb:3LVJ|rcsb pdb:3LVK|rcsb pdb:3LVL|rcsb pdb:3LVM	psi-mi:"MI:0465"(dip)	-	-	full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2017/08/06	2017/08/10	rogid:mMbGJa3Kv9wt+Wof0tz+8Yhhf9Q83334	rogid:BbF93e6Jza5CijszN1IFt/7B2BY83334	rigid:3iQZHP7THT9N9hfhZ8qbMZutSq4	false	-	-	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
uniprotkb:P0A892	uniprotkb:P0A6B9	intact:EBI-15849930|ensemblbacteria:AAG58579|ensemblbacteria:BAB37742|uniprotkb:P37618	intact:EBI-9011195|uniprotkb:P39171|uniprotkb:P76581|uniprotkb:P76992|uniprotkb:Q8XA86|ensemblbacteria:AAG57644|ensemblbacteria:BAB36819	psi-mi:tusa_eco57(display_long)|uniprotkb:tusA(gene name)|psi-mi:tusA(display_short)|uniprotkb:Sulfur mediator TusA(gene name synonym)|uniprotkb:Sulfur transfer protein TusA(gene name synonym)|uniprotkb:tRNA 2-thiouridine synthesizing protein A(gene name synonym)|uniprotkb:ECs4319(locus name)|uniprotkb:Z4844(locus name)	psi-mi:iscs_eco57(display_long)|uniprotkb:iscS(gene name)|psi-mi:iscS(display_short)|uniprotkb:ECs3396(locus name)|uniprotkb:Z3797(locus name)	psi-mi:"MI:0114"(x-ray crystallography)	Shi et al. (2010)	pubmed:20404999|doi:10.1371/journal.pbio.1000354|imex:IM-15204	taxid:83334(eco57)|taxid:83334("Escherichia coli O157:H7")	taxid:83334(eco57)|taxid:83334("Escherichia coli O157:H7")	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0465"(DIP)	intact:EBI-15849970|imex:IM-15204-2|dip:DIP-185636E	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	ensemblbacteria:AAG58579(transcript)|ensemblbacteria:BAB37742(transcript)|ensemblbacteria:ECs_4319(gene)|ensemblbacteria:Z4844(gene)|go:"GO:0002143"(tRNA wobble position uridine thiolation)|go:"GO:0097163"(sulfur carrier activity)|interpro:IPR001455(SirA-like)|interpro:IPR022931|interpro:IPR036868|rcsb pdb:3LVJ|rcsb pdb:3LVK|go:"GO:0005737"(cytoplasm)|refseq:NP_312346.1|refseq:WP_000130621.1|dip:DIP-58574N	refseq:NP_311423.2|dip:DIP-58573N|refseq:WP_001295373.1|ensemblbacteria:AAG57644(transcript)|ensemblbacteria:BAB36819(transcript)|ensemblbacteria:ECs_3396(gene)|ensemblbacteria:Z3797(gene)|go:"GO:0005737"(cytoplasm)|go:"GO:0008033"(tRNA processing)|go:"GO:0030170"(pyridoxal phosphate binding)|go:"GO:0031071"(cysteine desulfurase activity)|go:"GO:0044571"([2Fe-2S] cluster assembly)|go:"GO:0046872"(metal ion binding)|go:"GO:0051537"(2 iron, 2 sulfur cluster binding)|interpro:IPR000192(Aminotransferase, class V/Cysteine desulphurase)|interpro:IPR010240(Cysteine desulphurase)|interpro:IPR015421(Pyridoxal phosphate-dependent transferase, major region, subdomain 1)|interpro:IPR015422(Pyridoxal phosphate-dependent transferase, major region, subdomain 2)|interpro:IPR015424(Pyridoxal phosphate-dependent transferase, major region)|interpro:IPR016454(Cysteine desulphurase, NifS)|interpro:IPR020578|rcsb pdb:3LVJ|rcsb pdb:3LVK|rcsb pdb:3LVL|rcsb pdb:3LVM	psi-mi:"MI:0465"(dip)	-	-	full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2017/08/06	2017/08/10	rogid:mMbGJa3Kv9wt+Wof0tz+8Yhhf9Q83334	rogid:BbF93e6Jza5CijszN1IFt/7B2BY83334	rigid:3iQZHP7THT9N9hfhZ8qbMZutSq4	false	-	-	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
uniprotkb:P0C0M0	uniprotkb:P0ACD6	intact:EBI-15849987|ensemblbacteria:AAG57638|ensemblbacteria:BAB36813|uniprotkb:P37096	intact:EBI-9011202|uniprotkb:P77310|ensemblbacteria:AAG57643|ensemblbacteria:BAB36818	psi-mi:iscx_eco57(display_long)|uniprotkb:iscX(gene name)|psi-mi:iscX(display_short)|uniprotkb:Z3791(locus name)|uniprotkb:ECs3390(locus name)	psi-mi:iscu_eco57(display_long)|uniprotkb:iscU(gene name)|psi-mi:iscU(display_short)|uniprotkb:nifU(gene name synonym)|uniprotkb:ECs3395(locus name)|uniprotkb:Z3796(locus name)|uniprotkb:Sulfur acceptor protein IscU(gene name synonym)	psi-mi:"MI:0096"(pull down)	Shi et al. (2010)	pubmed:20404999|doi:10.1371/journal.pbio.1000354|imex:IM-15204	taxid:83334(eco57)|taxid:83334("Escherichia coli O157:H7")	taxid:83334(eco57)|taxid:83334("Escherichia coli O157:H7")	psi-mi:"MI:0914"(association)	psi-mi:"MI:0465"(DIP)	intact:EBI-15850000|imex:IM-15204-3|dip:DIP-185643E	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	ensemblbacteria:AAG57638(transcript)|ensemblbacteria:BAB36813(transcript)|ensemblbacteria:ECs_3390(gene)|ensemblbacteria:Z3791(gene)|go:"GO:0016226"(iron-sulfur cluster assembly)|interpro:IPR007479(ISC system FeS cluster assembly, IscX)|interpro:IPR036762|refseq:NP_311417.1|refseq:WP_000523616.1|dip:DIP-58578N	refseq:NP_311422.1|dip:DIP-58575N|refseq:WP_000331707.1|ensemblbacteria:AAG57643(transcript)|ensemblbacteria:BAB36818(transcript)|ensemblbacteria:ECs_3395(gene)|ensemblbacteria:Z3796(gene)|go:"GO:0005506"(iron ion binding)|go:"GO:0016226"(iron-sulfur cluster assembly)|go:"GO:0051536"(iron-sulfur cluster binding)|interpro:IPR002871(NIF system FeS cluster assembly, NifU, N-terminal)|interpro:IPR011339(ISC system FeS cluster assembly, IscU scaffold)|rcsb pdb:3LVL	psi-mi:"MI:0465"(dip)	-	-	full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2017/08/06	2017/08/10	rogid:hN/e9GJssPLkBuo/K6yoa5e4Q2083334	rogid:2JmTdVPfAy33rAim10pVQ33gJrI83334	rigid:MHRB+u3hDSTy80d96VwNBoVeoXE	false	glutathione s tranferase tag:n-n	-	-	-	psi-mi:"MI:0818"(molecular weight estimation by coomasie staining)	psi-mi:"MI:0818"(molecular weight estimation by coomasie staining)
uniprotkb:P0C0M0	uniprotkb:P0A6B9	intact:EBI-15849987|ensemblbacteria:AAG57638|ensemblbacteria:BAB36813|uniprotkb:P37096	intact:EBI-9011195|uniprotkb:P39171|uniprotkb:P76581|uniprotkb:P76992|uniprotkb:Q8XA86|ensemblbacteria:AAG57644|ensemblbacteria:BAB36819	psi-mi:iscx_eco57(display_long)|uniprotkb:iscX(gene name)|psi-mi:iscX(display_short)|uniprotkb:Z3791(locus name)|uniprotkb:ECs3390(locus name)	psi-mi:iscs_eco57(display_long)|uniprotkb:iscS(gene name)|psi-mi:iscS(display_short)|uniprotkb:ECs3396(locus name)|uniprotkb:Z3797(locus name)	psi-mi:"MI:0096"(pull down)	Shi et al. (2010)	pubmed:20404999|doi:10.1371/journal.pbio.1000354|imex:IM-15204	taxid:83334(eco57)|taxid:83334("Escherichia coli O157:H7")	taxid:83334(eco57)|taxid:83334("Escherichia coli O157:H7")	psi-mi:"MI:0914"(association)	psi-mi:"MI:0465"(DIP)	intact:EBI-15850000|imex:IM-15204-3|dip:DIP-185643E	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	ensemblbacteria:AAG57638(transcript)|ensemblbacteria:BAB36813(transcript)|ensemblbacteria:ECs_3390(gene)|ensemblbacteria:Z3791(gene)|go:"GO:0016226"(iron-sulfur cluster assembly)|interpro:IPR007479(ISC system FeS cluster assembly, IscX)|interpro:IPR036762|refseq:NP_311417.1|refseq:WP_000523616.1|dip:DIP-58578N	refseq:NP_311423.2|dip:DIP-58573N|refseq:WP_001295373.1|ensemblbacteria:AAG57644(transcript)|ensemblbacteria:BAB36819(transcript)|ensemblbacteria:ECs_3396(gene)|ensemblbacteria:Z3797(gene)|go:"GO:0005737"(cytoplasm)|go:"GO:0008033"(tRNA processing)|go:"GO:0030170"(pyridoxal phosphate binding)|go:"GO:0031071"(cysteine desulfurase activity)|go:"GO:0044571"([2Fe-2S] cluster assembly)|go:"GO:0046872"(metal ion binding)|go:"GO:0051537"(2 iron, 2 sulfur cluster binding)|interpro:IPR000192(Aminotransferase, class V/Cysteine desulphurase)|interpro:IPR010240(Cysteine desulphurase)|interpro:IPR015421(Pyridoxal phosphate-dependent transferase, major region, subdomain 1)|interpro:IPR015422(Pyridoxal phosphate-dependent transferase, major region, subdomain 2)|interpro:IPR015424(Pyridoxal phosphate-dependent transferase, major region)|interpro:IPR016454(Cysteine desulphurase, NifS)|interpro:IPR020578|rcsb pdb:3LVJ|rcsb pdb:3LVK|rcsb pdb:3LVL|rcsb pdb:3LVM	psi-mi:"MI:0465"(dip)	-	-	full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2017/08/06	2017/08/10	rogid:hN/e9GJssPLkBuo/K6yoa5e4Q2083334	rogid:BbF93e6Jza5CijszN1IFt/7B2BY83334	rigid:MHRB+u3hDSTy80d96VwNBoVeoXE	false	glutathione s tranferase tag:n-n	-	-	-	psi-mi:"MI:0818"(molecular weight estimation by coomasie staining)	psi-mi:"MI:0818"(molecular weight estimation by coomasie staining)
uniprotkb:P0ACD6	uniprotkb:P0A6B9	intact:EBI-9011202|uniprotkb:P77310|ensemblbacteria:AAG57643|ensemblbacteria:BAB36818	intact:EBI-9011195|uniprotkb:P39171|uniprotkb:P76581|uniprotkb:P76992|uniprotkb:Q8XA86|ensemblbacteria:AAG57644|ensemblbacteria:BAB36819	psi-mi:iscu_eco57(display_long)|uniprotkb:iscU(gene name)|psi-mi:iscU(display_short)|uniprotkb:nifU(gene name synonym)|uniprotkb:ECs3395(locus name)|uniprotkb:Z3796(locus name)|uniprotkb:Sulfur acceptor protein IscU(gene name synonym)	psi-mi:iscs_eco57(display_long)|uniprotkb:iscS(gene name)|psi-mi:iscS(display_short)|uniprotkb:ECs3396(locus name)|uniprotkb:Z3797(locus name)	psi-mi:"MI:0826"(x ray scattering)	Shi et al. (2010)	pubmed:20404999|doi:10.1371/journal.pbio.1000354|imex:IM-15204	taxid:83334(eco57)|taxid:83334("Escherichia coli O157:H7")	taxid:83334(eco57)|taxid:83334("Escherichia coli O157:H7")	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0465"(DIP)	intact:EBI-15850194|imex:IM-15204-8|dip:DIP-185637E	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_311422.1|dip:DIP-58575N|refseq:WP_000331707.1|ensemblbacteria:AAG57643(transcript)|ensemblbacteria:BAB36818(transcript)|ensemblbacteria:ECs_3395(gene)|ensemblbacteria:Z3796(gene)|go:"GO:0005506"(iron ion binding)|go:"GO:0016226"(iron-sulfur cluster assembly)|go:"GO:0051536"(iron-sulfur cluster binding)|interpro:IPR002871(NIF system FeS cluster assembly, NifU, N-terminal)|interpro:IPR011339(ISC system FeS cluster assembly, IscU scaffold)|rcsb pdb:3LVL	refseq:NP_311423.2|dip:DIP-58573N|refseq:WP_001295373.1|ensemblbacteria:AAG57644(transcript)|ensemblbacteria:BAB36819(transcript)|ensemblbacteria:ECs_3396(gene)|ensemblbacteria:Z3797(gene)|go:"GO:0005737"(cytoplasm)|go:"GO:0008033"(tRNA processing)|go:"GO:0030170"(pyridoxal phosphate binding)|go:"GO:0031071"(cysteine desulfurase activity)|go:"GO:0044571"([2Fe-2S] cluster assembly)|go:"GO:0046872"(metal ion binding)|go:"GO:0051537"(2 iron, 2 sulfur cluster binding)|interpro:IPR000192(Aminotransferase, class V/Cysteine desulphurase)|interpro:IPR010240(Cysteine desulphurase)|interpro:IPR015421(Pyridoxal phosphate-dependent transferase, major region, subdomain 1)|interpro:IPR015422(Pyridoxal phosphate-dependent transferase, major region, subdomain 2)|interpro:IPR015424(Pyridoxal phosphate-dependent transferase, major region)|interpro:IPR016454(Cysteine desulphurase, NifS)|interpro:IPR020578|rcsb pdb:3LVJ|rcsb pdb:3LVK|rcsb pdb:3LVL|rcsb pdb:3LVM	psi-mi:"MI:0465"(dip)	-	-	full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2017/08/06	2017/08/10	rogid:2JmTdVPfAy33rAim10pVQ33gJrI83334	rogid:BbF93e6Jza5CijszN1IFt/7B2BY83334	rigid:U1z1G0IbG3ksXRfTGboMPuZMELA	false	-	-	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
uniprotkb:P0A892	uniprotkb:P0A6B9	intact:EBI-15849930|ensemblbacteria:AAG58579|ensemblbacteria:BAB37742|uniprotkb:P37618	intact:EBI-9011195|uniprotkb:P39171|uniprotkb:P76581|uniprotkb:P76992|uniprotkb:Q8XA86|ensemblbacteria:AAG57644|ensemblbacteria:BAB36819	psi-mi:tusa_eco57(display_long)|uniprotkb:tusA(gene name)|psi-mi:tusA(display_short)|uniprotkb:Sulfur mediator TusA(gene name synonym)|uniprotkb:Sulfur transfer protein TusA(gene name synonym)|uniprotkb:tRNA 2-thiouridine synthesizing protein A(gene name synonym)|uniprotkb:ECs4319(locus name)|uniprotkb:Z4844(locus name)	psi-mi:iscs_eco57(display_long)|uniprotkb:iscS(gene name)|psi-mi:iscS(display_short)|uniprotkb:ECs3396(locus name)|uniprotkb:Z3797(locus name)	psi-mi:"MI:0096"(pull down)	Shi et al. (2010)	pubmed:20404999|doi:10.1371/journal.pbio.1000354|imex:IM-15204	taxid:83334(eco57)|taxid:83334("Escherichia coli O157:H7")	taxid:83334(eco57)|taxid:83334("Escherichia coli O157:H7")	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0465"(DIP)	intact:EBI-15850211|imex:IM-15204-9|dip:DIP-185636E	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	ensemblbacteria:AAG58579(transcript)|ensemblbacteria:BAB37742(transcript)|ensemblbacteria:ECs_4319(gene)|ensemblbacteria:Z4844(gene)|go:"GO:0002143"(tRNA wobble position uridine thiolation)|go:"GO:0097163"(sulfur carrier activity)|interpro:IPR001455(SirA-like)|interpro:IPR022931|interpro:IPR036868|rcsb pdb:3LVJ|rcsb pdb:3LVK|go:"GO:0005737"(cytoplasm)|refseq:NP_312346.1|refseq:WP_000130621.1|dip:DIP-58574N	refseq:NP_311423.2|dip:DIP-58573N|refseq:WP_001295373.1|ensemblbacteria:AAG57644(transcript)|ensemblbacteria:BAB36819(transcript)|ensemblbacteria:ECs_3396(gene)|ensemblbacteria:Z3797(gene)|go:"GO:0005737"(cytoplasm)|go:"GO:0008033"(tRNA processing)|go:"GO:0030170"(pyridoxal phosphate binding)|go:"GO:0031071"(cysteine desulfurase activity)|go:"GO:0044571"([2Fe-2S] cluster assembly)|go:"GO:0046872"(metal ion binding)|go:"GO:0051537"(2 iron, 2 sulfur cluster binding)|interpro:IPR000192(Aminotransferase, class V/Cysteine desulphurase)|interpro:IPR010240(Cysteine desulphurase)|interpro:IPR015421(Pyridoxal phosphate-dependent transferase, major region, subdomain 1)|interpro:IPR015422(Pyridoxal phosphate-dependent transferase, major region, subdomain 2)|interpro:IPR015424(Pyridoxal phosphate-dependent transferase, major region)|interpro:IPR016454(Cysteine desulphurase, NifS)|interpro:IPR020578|rcsb pdb:3LVJ|rcsb pdb:3LVK|rcsb pdb:3LVL|rcsb pdb:3LVM	psi-mi:"MI:0465"(dip)	-	-	full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2017/08/06	2017/08/10	rogid:mMbGJa3Kv9wt+Wof0tz+8Yhhf9Q83334	rogid:BbF93e6Jza5CijszN1IFt/7B2BY83334	rigid:3iQZHP7THT9N9hfhZ8qbMZutSq4	false	glutathione s tranferase tag:n-n	mutation disrupting interaction:45-45|mutation disrupting interaction:49-49|mutation disrupting interaction strength:52-52|mutation decreasing interaction:340-340|mutation decreasing interaction:65-65|mutation disrupting interaction strength:52-52|mutation disrupting interaction strength:52-52	-	-	psi-mi:"MI:0818"(molecular weight estimation by coomasie staining)	psi-mi:"MI:0818"(molecular weight estimation by coomasie staining)
uniprotkb:P0A892	uniprotkb:P0A6B9	intact:EBI-15849930|ensemblbacteria:AAG58579|ensemblbacteria:BAB37742|uniprotkb:P37618	intact:EBI-9011195|uniprotkb:P39171|uniprotkb:P76581|uniprotkb:P76992|uniprotkb:Q8XA86|ensemblbacteria:AAG57644|ensemblbacteria:BAB36819	psi-mi:tusa_eco57(display_long)|uniprotkb:tusA(gene name)|psi-mi:tusA(display_short)|uniprotkb:Sulfur mediator TusA(gene name synonym)|uniprotkb:Sulfur transfer protein TusA(gene name synonym)|uniprotkb:tRNA 2-thiouridine synthesizing protein A(gene name synonym)|uniprotkb:ECs4319(locus name)|uniprotkb:Z4844(locus name)	psi-mi:iscs_eco57(display_long)|uniprotkb:iscS(gene name)|psi-mi:iscS(display_short)|uniprotkb:ECs3396(locus name)|uniprotkb:Z3797(locus name)	psi-mi:"MI:0071"(molecular sieving)	Shi et al. (2010)	pubmed:20404999|doi:10.1371/journal.pbio.1000354|imex:IM-15204	taxid:83334(eco57)|taxid:83334("Escherichia coli O157:H7")	taxid:83334(eco57)|taxid:83334("Escherichia coli O157:H7")	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0465"(DIP)	intact:EBI-15850157|imex:IM-15204-6|dip:DIP-185636E	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	ensemblbacteria:AAG58579(transcript)|ensemblbacteria:BAB37742(transcript)|ensemblbacteria:ECs_4319(gene)|ensemblbacteria:Z4844(gene)|go:"GO:0002143"(tRNA wobble position uridine thiolation)|go:"GO:0097163"(sulfur carrier activity)|interpro:IPR001455(SirA-like)|interpro:IPR022931|interpro:IPR036868|rcsb pdb:3LVJ|rcsb pdb:3LVK|go:"GO:0005737"(cytoplasm)|refseq:NP_312346.1|refseq:WP_000130621.1|dip:DIP-58574N	refseq:NP_311423.2|dip:DIP-58573N|refseq:WP_001295373.1|ensemblbacteria:AAG57644(transcript)|ensemblbacteria:BAB36819(transcript)|ensemblbacteria:ECs_3396(gene)|ensemblbacteria:Z3797(gene)|go:"GO:0005737"(cytoplasm)|go:"GO:0008033"(tRNA processing)|go:"GO:0030170"(pyridoxal phosphate binding)|go:"GO:0031071"(cysteine desulfurase activity)|go:"GO:0044571"([2Fe-2S] cluster assembly)|go:"GO:0046872"(metal ion binding)|go:"GO:0051537"(2 iron, 2 sulfur cluster binding)|interpro:IPR000192(Aminotransferase, class V/Cysteine desulphurase)|interpro:IPR010240(Cysteine desulphurase)|interpro:IPR015421(Pyridoxal phosphate-dependent transferase, major region, subdomain 1)|interpro:IPR015422(Pyridoxal phosphate-dependent transferase, major region, subdomain 2)|interpro:IPR015424(Pyridoxal phosphate-dependent transferase, major region)|interpro:IPR016454(Cysteine desulphurase, NifS)|interpro:IPR020578|rcsb pdb:3LVJ|rcsb pdb:3LVK|rcsb pdb:3LVL|rcsb pdb:3LVM	psi-mi:"MI:0465"(dip)	-	-	full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2017/08/06	2017/08/10	rogid:mMbGJa3Kv9wt+Wof0tz+8Yhhf9Q83334	rogid:BbF93e6Jza5CijszN1IFt/7B2BY83334	rigid:3iQZHP7THT9N9hfhZ8qbMZutSq4	false	-	-	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
uniprotkb:Q8XAP0	uniprotkb:P0ACD6	intact:EBI-15850020|ensemblbacteria:AAG58999|ensemblbacteria:BAB38160	intact:EBI-9011202|uniprotkb:P77310|ensemblbacteria:AAG57643|ensemblbacteria:BAB36818	psi-mi:cyay_eco57(display_long)|uniprotkb:cyaY(gene name)|psi-mi:cyaY(display_short)|uniprotkb:Z5323(locus name)|uniprotkb:ECs4738(locus name)	psi-mi:iscu_eco57(display_long)|uniprotkb:iscU(gene name)|psi-mi:iscU(display_short)|uniprotkb:nifU(gene name synonym)|uniprotkb:ECs3395(locus name)|uniprotkb:Z3796(locus name)|uniprotkb:Sulfur acceptor protein IscU(gene name synonym)	psi-mi:"MI:0096"(pull down)	Shi et al. (2010)	pubmed:20404999|doi:10.1371/journal.pbio.1000354|imex:IM-15204	taxid:83334(eco57)|taxid:83334("Escherichia coli O157:H7")	taxid:83334(eco57)|taxid:83334("Escherichia coli O157:H7")	psi-mi:"MI:0914"(association)	psi-mi:"MI:0465"(DIP)	intact:EBI-15850174|imex:IM-15204-7|dip:DIP-185642E	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	ensemblbacteria:AAG58999(transcript)|ensemblbacteria:BAB38160(transcript)|ensemblbacteria:ECs_4738(gene)|ensemblbacteria:Z5323(gene)|go:"GO:0008199"(ferric iron binding)|go:"GO:0016226"(iron-sulfur cluster assembly)|interpro:IPR002908(Frataxin-like)|interpro:IPR020895|interpro:IPR036524|refseq:NP_312764.1|refseq:WP_000999944.1|dip:DIP-58577N	refseq:NP_311422.1|dip:DIP-58575N|refseq:WP_000331707.1|ensemblbacteria:AAG57643(transcript)|ensemblbacteria:BAB36818(transcript)|ensemblbacteria:ECs_3395(gene)|ensemblbacteria:Z3796(gene)|go:"GO:0005506"(iron ion binding)|go:"GO:0016226"(iron-sulfur cluster assembly)|go:"GO:0051536"(iron-sulfur cluster binding)|interpro:IPR002871(NIF system FeS cluster assembly, NifU, N-terminal)|interpro:IPR011339(ISC system FeS cluster assembly, IscU scaffold)|rcsb pdb:3LVL	psi-mi:"MI:0465"(dip)	-	-	full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2017/08/06	2017/08/10	rogid:suV2loqVU7Pf6f2gHEkxMBLswUs83334	rogid:2JmTdVPfAy33rAim10pVQ33gJrI83334	rigid:O+muPRc2jWdoTMSoccNu598HUME	false	glutathione s tranferase tag:n-n	-	-	-	psi-mi:"MI:0818"(molecular weight estimation by coomasie staining)	psi-mi:"MI:0818"(molecular weight estimation by coomasie staining)
uniprotkb:Q8XAP0	uniprotkb:P0A6B9	intact:EBI-15850020|ensemblbacteria:AAG58999|ensemblbacteria:BAB38160	intact:EBI-9011195|uniprotkb:P39171|uniprotkb:P76581|uniprotkb:P76992|uniprotkb:Q8XA86|ensemblbacteria:AAG57644|ensemblbacteria:BAB36819	psi-mi:cyay_eco57(display_long)|uniprotkb:cyaY(gene name)|psi-mi:cyaY(display_short)|uniprotkb:Z5323(locus name)|uniprotkb:ECs4738(locus name)	psi-mi:iscs_eco57(display_long)|uniprotkb:iscS(gene name)|psi-mi:iscS(display_short)|uniprotkb:ECs3396(locus name)|uniprotkb:Z3797(locus name)	psi-mi:"MI:0096"(pull down)	Shi et al. (2010)	pubmed:20404999|doi:10.1371/journal.pbio.1000354|imex:IM-15204	taxid:83334(eco57)|taxid:83334("Escherichia coli O157:H7")	taxid:83334(eco57)|taxid:83334("Escherichia coli O157:H7")	psi-mi:"MI:0914"(association)	psi-mi:"MI:0465"(DIP)	intact:EBI-15850174|imex:IM-15204-7|dip:DIP-185642E	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	ensemblbacteria:AAG58999(transcript)|ensemblbacteria:BAB38160(transcript)|ensemblbacteria:ECs_4738(gene)|ensemblbacteria:Z5323(gene)|go:"GO:0008199"(ferric iron binding)|go:"GO:0016226"(iron-sulfur cluster assembly)|interpro:IPR002908(Frataxin-like)|interpro:IPR020895|interpro:IPR036524|refseq:NP_312764.1|refseq:WP_000999944.1|dip:DIP-58577N	refseq:NP_311423.2|dip:DIP-58573N|refseq:WP_001295373.1|ensemblbacteria:AAG57644(transcript)|ensemblbacteria:BAB36819(transcript)|ensemblbacteria:ECs_3396(gene)|ensemblbacteria:Z3797(gene)|go:"GO:0005737"(cytoplasm)|go:"GO:0008033"(tRNA processing)|go:"GO:0030170"(pyridoxal phosphate binding)|go:"GO:0031071"(cysteine desulfurase activity)|go:"GO:0044571"([2Fe-2S] cluster assembly)|go:"GO:0046872"(metal ion binding)|go:"GO:0051537"(2 iron, 2 sulfur cluster binding)|interpro:IPR000192(Aminotransferase, class V/Cysteine desulphurase)|interpro:IPR010240(Cysteine desulphurase)|interpro:IPR015421(Pyridoxal phosphate-dependent transferase, major region, subdomain 1)|interpro:IPR015422(Pyridoxal phosphate-dependent transferase, major region, subdomain 2)|interpro:IPR015424(Pyridoxal phosphate-dependent transferase, major region)|interpro:IPR016454(Cysteine desulphurase, NifS)|interpro:IPR020578|rcsb pdb:3LVJ|rcsb pdb:3LVK|rcsb pdb:3LVL|rcsb pdb:3LVM	psi-mi:"MI:0465"(dip)	-	-	full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2017/08/06	2017/08/10	rogid:suV2loqVU7Pf6f2gHEkxMBLswUs83334	rogid:BbF93e6Jza5CijszN1IFt/7B2BY83334	rigid:O+muPRc2jWdoTMSoccNu598HUME	false	glutathione s tranferase tag:n-n	-	-	-	psi-mi:"MI:0818"(molecular weight estimation by coomasie staining)	psi-mi:"MI:0818"(molecular weight estimation by coomasie staining)
uniprotkb:Q8XAP0	uniprotkb:P0A6B9	intact:EBI-15850020|ensemblbacteria:AAG58999|ensemblbacteria:BAB38160	intact:EBI-9011195|uniprotkb:P39171|uniprotkb:P76581|uniprotkb:P76992|uniprotkb:Q8XA86|ensemblbacteria:AAG57644|ensemblbacteria:BAB36819	psi-mi:cyay_eco57(display_long)|uniprotkb:cyaY(gene name)|psi-mi:cyaY(display_short)|uniprotkb:Z5323(locus name)|uniprotkb:ECs4738(locus name)	psi-mi:iscs_eco57(display_long)|uniprotkb:iscS(gene name)|psi-mi:iscS(display_short)|uniprotkb:ECs3396(locus name)|uniprotkb:Z3797(locus name)	psi-mi:"MI:0096"(pull down)	Shi et al. (2010)	pubmed:20404999|doi:10.1371/journal.pbio.1000354|imex:IM-15204	taxid:83334(eco57)|taxid:83334("Escherichia coli O157:H7")	taxid:83334(eco57)|taxid:83334("Escherichia coli O157:H7")	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0465"(DIP)	intact:EBI-15850034|imex:IM-15204-4|dip:DIP-185640E	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	ensemblbacteria:AAG58999(transcript)|ensemblbacteria:BAB38160(transcript)|ensemblbacteria:ECs_4738(gene)|ensemblbacteria:Z5323(gene)|go:"GO:0008199"(ferric iron binding)|go:"GO:0016226"(iron-sulfur cluster assembly)|interpro:IPR002908(Frataxin-like)|interpro:IPR020895|interpro:IPR036524|refseq:NP_312764.1|refseq:WP_000999944.1|dip:DIP-58577N	refseq:NP_311423.2|dip:DIP-58573N|refseq:WP_001295373.1|ensemblbacteria:AAG57644(transcript)|ensemblbacteria:BAB36819(transcript)|ensemblbacteria:ECs_3396(gene)|ensemblbacteria:Z3797(gene)|go:"GO:0005737"(cytoplasm)|go:"GO:0008033"(tRNA processing)|go:"GO:0030170"(pyridoxal phosphate binding)|go:"GO:0031071"(cysteine desulfurase activity)|go:"GO:0044571"([2Fe-2S] cluster assembly)|go:"GO:0046872"(metal ion binding)|go:"GO:0051537"(2 iron, 2 sulfur cluster binding)|interpro:IPR000192(Aminotransferase, class V/Cysteine desulphurase)|interpro:IPR010240(Cysteine desulphurase)|interpro:IPR015421(Pyridoxal phosphate-dependent transferase, major region, subdomain 1)|interpro:IPR015422(Pyridoxal phosphate-dependent transferase, major region, subdomain 2)|interpro:IPR015424(Pyridoxal phosphate-dependent transferase, major region)|interpro:IPR016454(Cysteine desulphurase, NifS)|interpro:IPR020578|rcsb pdb:3LVJ|rcsb pdb:3LVK|rcsb pdb:3LVL|rcsb pdb:3LVM	psi-mi:"MI:0465"(dip)	-	-	full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2017/08/06	2017/08/10	rogid:suV2loqVU7Pf6f2gHEkxMBLswUs83334	rogid:BbF93e6Jza5CijszN1IFt/7B2BY83334	rigid:HToKwrjVbsbvvKgKZiRGgttAIaI	false	glutathione s tranferase tag:n-n	mutation disrupting interaction:239-239,237-237|mutation disrupting interaction:227-227,225-225|mutation disrupting interaction:340-340|mutation decreasing interaction:327-327|mutation decreasing interaction:234-234|mutation disrupting interaction:220-220|mutation disrupting interaction:223-223|mutation decreasing interaction:116-116|mutation decreasing interaction:39-39	-	-	psi-mi:"MI:0818"(molecular weight estimation by coomasie staining)	psi-mi:"MI:0818"(molecular weight estimation by coomasie staining)
uniprotkb:Q8XE74	uniprotkb:P0A6B9	intact:EBI-15850082|ensemblbacteria:AAG54773|ensemblbacteria:BAB33900	intact:EBI-9011195|uniprotkb:P39171|uniprotkb:P76581|uniprotkb:P76992|uniprotkb:Q8XA86|ensemblbacteria:AAG57644|ensemblbacteria:BAB36819	psi-mi:thii_eco57(display_long)|uniprotkb:thiI(gene name)|psi-mi:thiI(display_short)|uniprotkb:Sulfur carrier protein ThiS sulfurtransferase(gene name synonym)|uniprotkb:Thiamine biosynthesis protein ThiI(gene name synonym)|uniprotkb:tRNA 4-thiouridine synthase(gene name synonym)|uniprotkb:ECs0477(locus name)|uniprotkb:Z0526(locus name)	psi-mi:iscs_eco57(display_long)|uniprotkb:iscS(gene name)|psi-mi:iscS(display_short)|uniprotkb:ECs3396(locus name)|uniprotkb:Z3797(locus name)	psi-mi:"MI:0096"(pull down)	Shi et al. (2010)	pubmed:20404999|doi:10.1371/journal.pbio.1000354|imex:IM-15204	taxid:83334(eco57)|taxid:83334("Escherichia coli O157:H7")	taxid:83334(eco57)|taxid:83334("Escherichia coli O157:H7")	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0465"(DIP)	intact:EBI-15850110|imex:IM-15204-5|dip:DIP-185638E	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	ensemblbacteria:AAG54773(transcript)|ensemblbacteria:BAB33900(transcript)|ensemblbacteria:ECs_0477(gene)|ensemblbacteria:Z0526(gene)|go:"GO:0000049"(tRNA binding)|go:"GO:0004810"(tRNA adenylyltransferase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005737"(cytoplasm)|go:"GO:0009228"(thiamine biosynthetic process)|go:"GO:0009229"(thiamine diphosphate biosynthetic process)|go:"GO:0016783"(sulfurtransferase activity)|go:"GO:0034227"(tRNA thio-modification)|go:"GO:0052837"(thiazole biosynthetic process)|interpro:IPR001763(Rhodanese-like)|interpro:IPR003720(Thiamine biosynthesis protein)|interpro:IPR004114(THUMP)|interpro:IPR014729(Rossmann-like alpha/beta/alpha sandwich fold)|interpro:IPR020536|interpro:IPR026340|interpro:IPR036873|refseq:NP_308504.1|refseq:WP_000668700.1|dip:DIP-58576N	refseq:NP_311423.2|dip:DIP-58573N|refseq:WP_001295373.1|ensemblbacteria:AAG57644(transcript)|ensemblbacteria:BAB36819(transcript)|ensemblbacteria:ECs_3396(gene)|ensemblbacteria:Z3797(gene)|go:"GO:0005737"(cytoplasm)|go:"GO:0008033"(tRNA processing)|go:"GO:0030170"(pyridoxal phosphate binding)|go:"GO:0031071"(cysteine desulfurase activity)|go:"GO:0044571"([2Fe-2S] cluster assembly)|go:"GO:0046872"(metal ion binding)|go:"GO:0051537"(2 iron, 2 sulfur cluster binding)|interpro:IPR000192(Aminotransferase, class V/Cysteine desulphurase)|interpro:IPR010240(Cysteine desulphurase)|interpro:IPR015421(Pyridoxal phosphate-dependent transferase, major region, subdomain 1)|interpro:IPR015422(Pyridoxal phosphate-dependent transferase, major region, subdomain 2)|interpro:IPR015424(Pyridoxal phosphate-dependent transferase, major region)|interpro:IPR016454(Cysteine desulphurase, NifS)|interpro:IPR020578|rcsb pdb:3LVJ|rcsb pdb:3LVK|rcsb pdb:3LVL|rcsb pdb:3LVM	psi-mi:"MI:0465"(dip)	-	-	full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2017/08/06	2017/08/10	rogid:Jl2qrBzN8/O9PATzD71NYQic0rA83334	rogid:BbF93e6Jza5CijszN1IFt/7B2BY83334	rigid:n8cyPAVUwmd1+Zib74egH6v4Jq8	false	glutathione s tranferase tag:n-n	mutation decreasing interaction:327-327|mutation decreasing interaction:311-311|mutation decreasing interaction:223-223|mutation decreasing interaction:116-116|mutation decreasing interaction:89-89|mutation decreasing interaction:45-45|mutation disrupting interaction:220-220|mutation disrupting interaction:239-239,237-237|mutation disrupting interaction:340-340	-	-	psi-mi:"MI:0818"(molecular weight estimation by coomasie staining)	psi-mi:"MI:0818"(molecular weight estimation by coomasie staining)
uniprotkb:P0C0M0	uniprotkb:P0A6B9	intact:EBI-15849987|ensemblbacteria:AAG57638|ensemblbacteria:BAB36813|uniprotkb:P37096	intact:EBI-9011195|uniprotkb:P39171|uniprotkb:P76581|uniprotkb:P76992|uniprotkb:Q8XA86|ensemblbacteria:AAG57644|ensemblbacteria:BAB36819	psi-mi:iscx_eco57(display_long)|uniprotkb:iscX(gene name)|psi-mi:iscX(display_short)|uniprotkb:Z3791(locus name)|uniprotkb:ECs3390(locus name)	psi-mi:iscs_eco57(display_long)|uniprotkb:iscS(gene name)|psi-mi:iscS(display_short)|uniprotkb:ECs3396(locus name)|uniprotkb:Z3797(locus name)	psi-mi:"MI:0096"(pull down)	Shi et al. (2010)	pubmed:20404999|doi:10.1371/journal.pbio.1000354|imex:IM-15204	taxid:83334(eco57)|taxid:83334("Escherichia coli O157:H7")	taxid:83334(eco57)|taxid:83334("Escherichia coli O157:H7")	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0465"(DIP)	intact:EBI-15850335|imex:IM-15204-14|dip:DIP-185639E	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	ensemblbacteria:AAG57638(transcript)|ensemblbacteria:BAB36813(transcript)|ensemblbacteria:ECs_3390(gene)|ensemblbacteria:Z3791(gene)|go:"GO:0016226"(iron-sulfur cluster assembly)|interpro:IPR007479(ISC system FeS cluster assembly, IscX)|interpro:IPR036762|refseq:NP_311417.1|refseq:WP_000523616.1|dip:DIP-58578N	refseq:NP_311423.2|dip:DIP-58573N|refseq:WP_001295373.1|ensemblbacteria:AAG57644(transcript)|ensemblbacteria:BAB36819(transcript)|ensemblbacteria:ECs_3396(gene)|ensemblbacteria:Z3797(gene)|go:"GO:0005737"(cytoplasm)|go:"GO:0008033"(tRNA processing)|go:"GO:0030170"(pyridoxal phosphate binding)|go:"GO:0031071"(cysteine desulfurase activity)|go:"GO:0044571"([2Fe-2S] cluster assembly)|go:"GO:0046872"(metal ion binding)|go:"GO:0051537"(2 iron, 2 sulfur cluster binding)|interpro:IPR000192(Aminotransferase, class V/Cysteine desulphurase)|interpro:IPR010240(Cysteine desulphurase)|interpro:IPR015421(Pyridoxal phosphate-dependent transferase, major region, subdomain 1)|interpro:IPR015422(Pyridoxal phosphate-dependent transferase, major region, subdomain 2)|interpro:IPR015424(Pyridoxal phosphate-dependent transferase, major region)|interpro:IPR016454(Cysteine desulphurase, NifS)|interpro:IPR020578|rcsb pdb:3LVJ|rcsb pdb:3LVK|rcsb pdb:3LVL|rcsb pdb:3LVM	psi-mi:"MI:0465"(dip)	-	-	full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2017/08/06	2017/08/10	rogid:hN/e9GJssPLkBuo/K6yoa5e4Q2083334	rogid:BbF93e6Jza5CijszN1IFt/7B2BY83334	rigid:MC0ym1MOb4ItccCmsTYQSfjcemg	false	glutathione s tranferase tag:n-n	mutation disrupting interaction:327-327|mutation decreasing interaction:234-234|mutation disrupting interaction:223-223|mutation decreasing interaction:116-116|mutation decreasing interaction:112-112|mutation disrupting interaction:227-227,225-225|mutation disrupting interaction:220-220|mutation disrupting interaction:239-239,237-237|mutation decreasing interaction:340-340|mutation disrupting interaction:225-225,223-223	-	-	psi-mi:"MI:0818"(molecular weight estimation by coomasie staining)	psi-mi:"MI:0818"(molecular weight estimation by coomasie staining)
uniprotkb:P0A6B9	uniprotkb:P0A6B9	intact:EBI-9011195|uniprotkb:P39171|uniprotkb:P76581|uniprotkb:P76992|uniprotkb:Q8XA86|ensemblbacteria:AAG57644|ensemblbacteria:BAB36819	intact:EBI-9011195|uniprotkb:P39171|uniprotkb:P76581|uniprotkb:P76992|uniprotkb:Q8XA86|ensemblbacteria:AAG57644|ensemblbacteria:BAB36819	psi-mi:iscs_eco57(display_long)|uniprotkb:iscS(gene name)|psi-mi:iscS(display_short)|uniprotkb:ECs3396(locus name)|uniprotkb:Z3797(locus name)	psi-mi:iscs_eco57(display_long)|uniprotkb:iscS(gene name)|psi-mi:iscS(display_short)|uniprotkb:ECs3396(locus name)|uniprotkb:Z3797(locus name)	psi-mi:"MI:0114"(x-ray crystallography)	Shi et al. (2010)	pubmed:20404999|doi:10.1371/journal.pbio.1000354|imex:IM-15204	taxid:83334(eco57)|taxid:83334("Escherichia coli O157:H7")	taxid:83334(eco57)|taxid:83334("Escherichia coli O157:H7")	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0465"(DIP)	intact:EBI-15850319|imex:IM-15204-13|dip:DIP-185641E	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_311423.2|dip:DIP-58573N|refseq:WP_001295373.1|ensemblbacteria:AAG57644(transcript)|ensemblbacteria:BAB36819(transcript)|ensemblbacteria:ECs_3396(gene)|ensemblbacteria:Z3797(gene)|go:"GO:0005737"(cytoplasm)|go:"GO:0008033"(tRNA processing)|go:"GO:0030170"(pyridoxal phosphate binding)|go:"GO:0031071"(cysteine desulfurase activity)|go:"GO:0044571"([2Fe-2S] cluster assembly)|go:"GO:0046872"(metal ion binding)|go:"GO:0051537"(2 iron, 2 sulfur cluster binding)|interpro:IPR000192(Aminotransferase, class V/Cysteine desulphurase)|interpro:IPR010240(Cysteine desulphurase)|interpro:IPR015421(Pyridoxal phosphate-dependent transferase, major region, subdomain 1)|interpro:IPR015422(Pyridoxal phosphate-dependent transferase, major region, subdomain 2)|interpro:IPR015424(Pyridoxal phosphate-dependent transferase, major region)|interpro:IPR016454(Cysteine desulphurase, NifS)|interpro:IPR020578|rcsb pdb:3LVJ|rcsb pdb:3LVK|rcsb pdb:3LVL|rcsb pdb:3LVM	refseq:NP_311423.2|dip:DIP-58573N|refseq:WP_001295373.1|ensemblbacteria:AAG57644(transcript)|ensemblbacteria:BAB36819(transcript)|ensemblbacteria:ECs_3396(gene)|ensemblbacteria:Z3797(gene)|go:"GO:0005737"(cytoplasm)|go:"GO:0008033"(tRNA processing)|go:"GO:0030170"(pyridoxal phosphate binding)|go:"GO:0031071"(cysteine desulfurase activity)|go:"GO:0044571"([2Fe-2S] cluster assembly)|go:"GO:0046872"(metal ion binding)|go:"GO:0051537"(2 iron, 2 sulfur cluster binding)|interpro:IPR000192(Aminotransferase, class V/Cysteine desulphurase)|interpro:IPR010240(Cysteine desulphurase)|interpro:IPR015421(Pyridoxal phosphate-dependent transferase, major region, subdomain 1)|interpro:IPR015422(Pyridoxal phosphate-dependent transferase, major region, subdomain 2)|interpro:IPR015424(Pyridoxal phosphate-dependent transferase, major region)|interpro:IPR016454(Cysteine desulphurase, NifS)|interpro:IPR020578|rcsb pdb:3LVJ|rcsb pdb:3LVK|rcsb pdb:3LVL|rcsb pdb:3LVM	psi-mi:"MI:0465"(dip)	-	-	full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:562(ecolx)|taxid:562(Escherichia coli)	-	2017/08/06	2017/08/10	rogid:BbF93e6Jza5CijszN1IFt/7B2BY83334	rogid:BbF93e6Jza5CijszN1IFt/7B2BY83334	rigid:INTPxr7RWOjwM1kziw/Ud8+sZp0	false	-	-	-	0	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
uniprotkb:P0ACD6	uniprotkb:P0A6B9	intact:EBI-9011202|uniprotkb:P77310|ensemblbacteria:AAG57643|ensemblbacteria:BAB36818	intact:EBI-9011195|uniprotkb:P39171|uniprotkb:P76581|uniprotkb:P76992|uniprotkb:Q8XA86|ensemblbacteria:AAG57644|ensemblbacteria:BAB36819	psi-mi:iscu_eco57(display_long)|uniprotkb:iscU(gene name)|psi-mi:iscU(display_short)|uniprotkb:nifU(gene name synonym)|uniprotkb:ECs3395(locus name)|uniprotkb:Z3796(locus name)|uniprotkb:Sulfur acceptor protein IscU(gene name synonym)	psi-mi:iscs_eco57(display_long)|uniprotkb:iscS(gene name)|psi-mi:iscS(display_short)|uniprotkb:ECs3396(locus name)|uniprotkb:Z3797(locus name)	psi-mi:"MI:0071"(molecular sieving)	Shi et al. (2010)	pubmed:20404999|doi:10.1371/journal.pbio.1000354|imex:IM-15204	taxid:83334(eco57)|taxid:83334("Escherichia coli O157:H7")	taxid:83334(eco57)|taxid:83334("Escherichia coli O157:H7")	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0465"(DIP)	intact:EBI-15850301|imex:IM-15204-12|dip:DIP-185637E	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_311422.1|dip:DIP-58575N|refseq:WP_000331707.1|ensemblbacteria:AAG57643(transcript)|ensemblbacteria:BAB36818(transcript)|ensemblbacteria:ECs_3395(gene)|ensemblbacteria:Z3796(gene)|go:"GO:0005506"(iron ion binding)|go:"GO:0016226"(iron-sulfur cluster assembly)|go:"GO:0051536"(iron-sulfur cluster binding)|interpro:IPR002871(NIF system FeS cluster assembly, NifU, N-terminal)|interpro:IPR011339(ISC system FeS cluster assembly, IscU scaffold)|rcsb pdb:3LVL	refseq:NP_311423.2|dip:DIP-58573N|refseq:WP_001295373.1|ensemblbacteria:AAG57644(transcript)|ensemblbacteria:BAB36819(transcript)|ensemblbacteria:ECs_3396(gene)|ensemblbacteria:Z3797(gene)|go:"GO:0005737"(cytoplasm)|go:"GO:0008033"(tRNA processing)|go:"GO:0030170"(pyridoxal phosphate binding)|go:"GO:0031071"(cysteine desulfurase activity)|go:"GO:0044571"([2Fe-2S] cluster assembly)|go:"GO:0046872"(metal ion binding)|go:"GO:0051537"(2 iron, 2 sulfur cluster binding)|interpro:IPR000192(Aminotransferase, class V/Cysteine desulphurase)|interpro:IPR010240(Cysteine desulphurase)|interpro:IPR015421(Pyridoxal phosphate-dependent transferase, major region, subdomain 1)|interpro:IPR015422(Pyridoxal phosphate-dependent transferase, major region, subdomain 2)|interpro:IPR015424(Pyridoxal phosphate-dependent transferase, major region)|interpro:IPR016454(Cysteine desulphurase, NifS)|interpro:IPR020578|rcsb pdb:3LVJ|rcsb pdb:3LVK|rcsb pdb:3LVL|rcsb pdb:3LVM	psi-mi:"MI:0465"(dip)	-	-	full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2017/08/06	2017/08/10	rogid:2JmTdVPfAy33rAim10pVQ33gJrI83334	rogid:BbF93e6Jza5CijszN1IFt/7B2BY83334	rigid:U1z1G0IbG3ksXRfTGboMPuZMELA	false	-	-	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
uniprotkb:P0A892	uniprotkb:P0A6B9	intact:EBI-15849930|ensemblbacteria:AAG58579|ensemblbacteria:BAB37742|uniprotkb:P37618	intact:EBI-9011195|uniprotkb:P39171|uniprotkb:P76581|uniprotkb:P76992|uniprotkb:Q8XA86|ensemblbacteria:AAG57644|ensemblbacteria:BAB36819	psi-mi:tusa_eco57(display_long)|uniprotkb:tusA(gene name)|psi-mi:tusA(display_short)|uniprotkb:Sulfur mediator TusA(gene name synonym)|uniprotkb:Sulfur transfer protein TusA(gene name synonym)|uniprotkb:tRNA 2-thiouridine synthesizing protein A(gene name synonym)|uniprotkb:ECs4319(locus name)|uniprotkb:Z4844(locus name)	psi-mi:iscs_eco57(display_long)|uniprotkb:iscS(gene name)|psi-mi:iscS(display_short)|uniprotkb:ECs3396(locus name)|uniprotkb:Z3797(locus name)	psi-mi:"MI:0096"(pull down)	Shi et al. (2010)	pubmed:20404999|doi:10.1371/journal.pbio.1000354|imex:IM-15204	taxid:83334(eco57)|taxid:83334("Escherichia coli O157:H7")	taxid:83334(eco57)|taxid:83334("Escherichia coli O157:H7")	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0465"(DIP)	intact:EBI-15850266|imex:IM-15204-11|dip:DIP-185636E	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	ensemblbacteria:AAG58579(transcript)|ensemblbacteria:BAB37742(transcript)|ensemblbacteria:ECs_4319(gene)|ensemblbacteria:Z4844(gene)|go:"GO:0002143"(tRNA wobble position uridine thiolation)|go:"GO:0097163"(sulfur carrier activity)|interpro:IPR001455(SirA-like)|interpro:IPR022931|interpro:IPR036868|rcsb pdb:3LVJ|rcsb pdb:3LVK|go:"GO:0005737"(cytoplasm)|refseq:NP_312346.1|refseq:WP_000130621.1|dip:DIP-58574N	refseq:NP_311423.2|dip:DIP-58573N|refseq:WP_001295373.1|ensemblbacteria:AAG57644(transcript)|ensemblbacteria:BAB36819(transcript)|ensemblbacteria:ECs_3396(gene)|ensemblbacteria:Z3797(gene)|go:"GO:0005737"(cytoplasm)|go:"GO:0008033"(tRNA processing)|go:"GO:0030170"(pyridoxal phosphate binding)|go:"GO:0031071"(cysteine desulfurase activity)|go:"GO:0044571"([2Fe-2S] cluster assembly)|go:"GO:0046872"(metal ion binding)|go:"GO:0051537"(2 iron, 2 sulfur cluster binding)|interpro:IPR000192(Aminotransferase, class V/Cysteine desulphurase)|interpro:IPR010240(Cysteine desulphurase)|interpro:IPR015421(Pyridoxal phosphate-dependent transferase, major region, subdomain 1)|interpro:IPR015422(Pyridoxal phosphate-dependent transferase, major region, subdomain 2)|interpro:IPR015424(Pyridoxal phosphate-dependent transferase, major region)|interpro:IPR016454(Cysteine desulphurase, NifS)|interpro:IPR020578|rcsb pdb:3LVJ|rcsb pdb:3LVK|rcsb pdb:3LVL|rcsb pdb:3LVM	psi-mi:"MI:0465"(dip)	-	-	full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2017/08/06	2017/08/10	rogid:mMbGJa3Kv9wt+Wof0tz+8Yhhf9Q83334	rogid:BbF93e6Jza5CijszN1IFt/7B2BY83334	rigid:3iQZHP7THT9N9hfhZ8qbMZutSq4	false	glutathione s tranferase tag:n-n|mutation decreasing interaction:21-21|mutation disrupting interaction:24-24|mutation disrupting interaction strength:27-27|mutation disrupting interaction:31-31|mutation disrupting interaction:58-58|mutation disrupting interaction strength:27-27	-	-	-	psi-mi:"MI:0818"(molecular weight estimation by coomasie staining)	psi-mi:"MI:0818"(molecular weight estimation by coomasie staining)
uniprotkb:P0ACD6	uniprotkb:P0A6B9	intact:EBI-9011202|uniprotkb:P77310|ensemblbacteria:AAG57643|ensemblbacteria:BAB36818	intact:EBI-9011195|uniprotkb:P39171|uniprotkb:P76581|uniprotkb:P76992|uniprotkb:Q8XA86|ensemblbacteria:AAG57644|ensemblbacteria:BAB36819	psi-mi:iscu_eco57(display_long)|uniprotkb:iscU(gene name)|psi-mi:iscU(display_short)|uniprotkb:nifU(gene name synonym)|uniprotkb:ECs3395(locus name)|uniprotkb:Z3796(locus name)|uniprotkb:Sulfur acceptor protein IscU(gene name synonym)	psi-mi:iscs_eco57(display_long)|uniprotkb:iscS(gene name)|psi-mi:iscS(display_short)|uniprotkb:ECs3396(locus name)|uniprotkb:Z3797(locus name)	psi-mi:"MI:0114"(x-ray crystallography)	Shi et al. (2010)	pubmed:20404999|doi:10.1371/journal.pbio.1000354|imex:IM-15204	taxid:83334(eco57)|taxid:83334("Escherichia coli O157:H7")	taxid:83334(eco57)|taxid:83334("Escherichia coli O157:H7")	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0465"(DIP)	intact:EBI-15850249|imex:IM-15204-10|dip:DIP-185637E	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_311422.1|dip:DIP-58575N|refseq:WP_000331707.1|ensemblbacteria:AAG57643(transcript)|ensemblbacteria:BAB36818(transcript)|ensemblbacteria:ECs_3395(gene)|ensemblbacteria:Z3796(gene)|go:"GO:0005506"(iron ion binding)|go:"GO:0016226"(iron-sulfur cluster assembly)|go:"GO:0051536"(iron-sulfur cluster binding)|interpro:IPR002871(NIF system FeS cluster assembly, NifU, N-terminal)|interpro:IPR011339(ISC system FeS cluster assembly, IscU scaffold)|rcsb pdb:3LVL	refseq:NP_311423.2|dip:DIP-58573N|refseq:WP_001295373.1|ensemblbacteria:AAG57644(transcript)|ensemblbacteria:BAB36819(transcript)|ensemblbacteria:ECs_3396(gene)|ensemblbacteria:Z3797(gene)|go:"GO:0005737"(cytoplasm)|go:"GO:0008033"(tRNA processing)|go:"GO:0030170"(pyridoxal phosphate binding)|go:"GO:0031071"(cysteine desulfurase activity)|go:"GO:0044571"([2Fe-2S] cluster assembly)|go:"GO:0046872"(metal ion binding)|go:"GO:0051537"(2 iron, 2 sulfur cluster binding)|interpro:IPR000192(Aminotransferase, class V/Cysteine desulphurase)|interpro:IPR010240(Cysteine desulphurase)|interpro:IPR015421(Pyridoxal phosphate-dependent transferase, major region, subdomain 1)|interpro:IPR015422(Pyridoxal phosphate-dependent transferase, major region, subdomain 2)|interpro:IPR015424(Pyridoxal phosphate-dependent transferase, major region)|interpro:IPR016454(Cysteine desulphurase, NifS)|interpro:IPR020578|rcsb pdb:3LVJ|rcsb pdb:3LVK|rcsb pdb:3LVL|rcsb pdb:3LVM	psi-mi:"MI:0465"(dip)	-	-	full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2017/08/06	2017/08/10	rogid:2JmTdVPfAy33rAim10pVQ33gJrI83334	rogid:BbF93e6Jza5CijszN1IFt/7B2BY83334	rigid:U1z1G0IbG3ksXRfTGboMPuZMELA	false	-	-	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
uniprotkb:P0ACD6	uniprotkb:P0A6B9	intact:EBI-9011202|uniprotkb:P77310|ensemblbacteria:AAG57643|ensemblbacteria:BAB36818	intact:EBI-9011195|uniprotkb:P39171|uniprotkb:P76581|uniprotkb:P76992|uniprotkb:Q8XA86|ensemblbacteria:AAG57644|ensemblbacteria:BAB36819	psi-mi:iscu_eco57(display_long)|uniprotkb:iscU(gene name)|psi-mi:iscU(display_short)|uniprotkb:nifU(gene name synonym)|uniprotkb:ECs3395(locus name)|uniprotkb:Z3796(locus name)|uniprotkb:Sulfur acceptor protein IscU(gene name synonym)	psi-mi:iscs_eco57(display_long)|uniprotkb:iscS(gene name)|psi-mi:iscS(display_short)|uniprotkb:ECs3396(locus name)|uniprotkb:Z3797(locus name)	psi-mi:"MI:0096"(pull down)	Shi et al. (2010)	pubmed:20404999|doi:10.1371/journal.pbio.1000354|imex:IM-15204	taxid:83334(eco57)|taxid:83334("Escherichia coli O157:H7")	taxid:83334(eco57)|taxid:83334("Escherichia coli O157:H7")	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0465"(DIP)	intact:EBI-15850387|imex:IM-15204-15|dip:DIP-185637E	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_311422.1|dip:DIP-58575N|refseq:WP_000331707.1|ensemblbacteria:AAG57643(transcript)|ensemblbacteria:BAB36818(transcript)|ensemblbacteria:ECs_3395(gene)|ensemblbacteria:Z3796(gene)|go:"GO:0005506"(iron ion binding)|go:"GO:0016226"(iron-sulfur cluster assembly)|go:"GO:0051536"(iron-sulfur cluster binding)|interpro:IPR002871(NIF system FeS cluster assembly, NifU, N-terminal)|interpro:IPR011339(ISC system FeS cluster assembly, IscU scaffold)|rcsb pdb:3LVL	refseq:NP_311423.2|dip:DIP-58573N|refseq:WP_001295373.1|ensemblbacteria:AAG57644(transcript)|ensemblbacteria:BAB36819(transcript)|ensemblbacteria:ECs_3396(gene)|ensemblbacteria:Z3797(gene)|go:"GO:0005737"(cytoplasm)|go:"GO:0008033"(tRNA processing)|go:"GO:0030170"(pyridoxal phosphate binding)|go:"GO:0031071"(cysteine desulfurase activity)|go:"GO:0044571"([2Fe-2S] cluster assembly)|go:"GO:0046872"(metal ion binding)|go:"GO:0051537"(2 iron, 2 sulfur cluster binding)|interpro:IPR000192(Aminotransferase, class V/Cysteine desulphurase)|interpro:IPR010240(Cysteine desulphurase)|interpro:IPR015421(Pyridoxal phosphate-dependent transferase, major region, subdomain 1)|interpro:IPR015422(Pyridoxal phosphate-dependent transferase, major region, subdomain 2)|interpro:IPR015424(Pyridoxal phosphate-dependent transferase, major region)|interpro:IPR016454(Cysteine desulphurase, NifS)|interpro:IPR020578|rcsb pdb:3LVJ|rcsb pdb:3LVK|rcsb pdb:3LVL|rcsb pdb:3LVM	psi-mi:"MI:0465"(dip)	-	-	full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2017/08/06	2017/08/10	rogid:2JmTdVPfAy33rAim10pVQ33gJrI83334	rogid:BbF93e6Jza5CijszN1IFt/7B2BY83334	rigid:U1z1G0IbG3ksXRfTGboMPuZMELA	false	glutathione s tranferase tag:n-n|mutation disrupting interaction:103-103|sufficient binding region:1-12	-	-	-	psi-mi:"MI:0818"(molecular weight estimation by coomasie staining)	psi-mi:"MI:0818"(molecular weight estimation by coomasie staining)
uniprotkb:P0ACD6	uniprotkb:P0A6B9	intact:EBI-9011202|uniprotkb:P77310|ensemblbacteria:AAG57643|ensemblbacteria:BAB36818	intact:EBI-9011195|uniprotkb:P39171|uniprotkb:P76581|uniprotkb:P76992|uniprotkb:Q8XA86|ensemblbacteria:AAG57644|ensemblbacteria:BAB36819	psi-mi:iscu_eco57(display_long)|uniprotkb:iscU(gene name)|psi-mi:iscU(display_short)|uniprotkb:nifU(gene name synonym)|uniprotkb:ECs3395(locus name)|uniprotkb:Z3796(locus name)|uniprotkb:Sulfur acceptor protein IscU(gene name synonym)	psi-mi:iscs_eco57(display_long)|uniprotkb:iscS(gene name)|psi-mi:iscS(display_short)|uniprotkb:ECs3396(locus name)|uniprotkb:Z3797(locus name)	psi-mi:"MI:0096"(pull down)	Shi et al. (2010)	pubmed:20404999|doi:10.1371/journal.pbio.1000354|imex:IM-15204	taxid:83334(eco57)|taxid:83334("Escherichia coli O157:H7")	taxid:83334(eco57)|taxid:83334("Escherichia coli O157:H7")	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0465"(DIP)	intact:EBI-15850411|imex:IM-15204-16|dip:DIP-185637E	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_311422.1|dip:DIP-58575N|refseq:WP_000331707.1|ensemblbacteria:AAG57643(transcript)|ensemblbacteria:BAB36818(transcript)|ensemblbacteria:ECs_3395(gene)|ensemblbacteria:Z3796(gene)|go:"GO:0005506"(iron ion binding)|go:"GO:0016226"(iron-sulfur cluster assembly)|go:"GO:0051536"(iron-sulfur cluster binding)|interpro:IPR002871(NIF system FeS cluster assembly, NifU, N-terminal)|interpro:IPR011339(ISC system FeS cluster assembly, IscU scaffold)|rcsb pdb:3LVL	refseq:NP_311423.2|dip:DIP-58573N|refseq:WP_001295373.1|ensemblbacteria:AAG57644(transcript)|ensemblbacteria:BAB36819(transcript)|ensemblbacteria:ECs_3396(gene)|ensemblbacteria:Z3797(gene)|go:"GO:0005737"(cytoplasm)|go:"GO:0008033"(tRNA processing)|go:"GO:0030170"(pyridoxal phosphate binding)|go:"GO:0031071"(cysteine desulfurase activity)|go:"GO:0044571"([2Fe-2S] cluster assembly)|go:"GO:0046872"(metal ion binding)|go:"GO:0051537"(2 iron, 2 sulfur cluster binding)|interpro:IPR000192(Aminotransferase, class V/Cysteine desulphurase)|interpro:IPR010240(Cysteine desulphurase)|interpro:IPR015421(Pyridoxal phosphate-dependent transferase, major region, subdomain 1)|interpro:IPR015422(Pyridoxal phosphate-dependent transferase, major region, subdomain 2)|interpro:IPR015424(Pyridoxal phosphate-dependent transferase, major region)|interpro:IPR016454(Cysteine desulphurase, NifS)|interpro:IPR020578|rcsb pdb:3LVJ|rcsb pdb:3LVK|rcsb pdb:3LVL|rcsb pdb:3LVM	psi-mi:"MI:0465"(dip)	-	-	full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2017/08/06	2017/08/10	rogid:2JmTdVPfAy33rAim10pVQ33gJrI83334	rogid:BbF93e6Jza5CijszN1IFt/7B2BY83334	rigid:U1z1G0IbG3ksXRfTGboMPuZMELA	false	glutathione s tranferase tag:n-n	mutation decreasing interaction:327-327	-	-	psi-mi:"MI:0818"(molecular weight estimation by coomasie staining)	psi-mi:"MI:0818"(molecular weight estimation by coomasie staining)