#ID(s) interactor A	ID(s) interactor B	Alt. ID(s) interactor A	Alt. ID(s) interactor B	Alias(es) interactor A	Alias(es) interactor B	Interaction detection method(s)	Publication 1st author(s)	Publication Identifier(s)	Taxid interactor A	Taxid interactor B	Interaction type(s)	Source database(s)	Interaction identifier(s)	Confidence value(s)	Expansion method(s)	Biological role(s) interactor A	Biological role(s) interactor B	Experimental role(s) interactor A	Experimental role(s) interactor B	Type(s) interactor A	Type(s) interactor B	Xref(s) interactor A	Xref(s) interactor B	Interaction Xref(s)	Annotation(s) interactor A	Annotation(s) interactor B	Interaction annotation(s)	Host organism(s)	Interaction parameter(s)	Creation date	Update date	Checksum(s) interactor A	Checksum(s) interactor B	Interaction Checksum(s)	Negative	Feature(s) interactor A	Feature(s) interactor B	Stoichiometry(s) interactor A	Stoichiometry(s) interactor B	Identification method participant A	Identification method participant B
uniprotkb:P25656	uniprotkb:P39524	intact:EBI-22014|uniprotkb:D6VR94|ensemblfungi:YCR094W	intact:EBI-3106|uniprotkb:D6VPJ2|ensemblfungi:YAL026C	psi-mi:cdc50_yeast(display_long)|uniprotkb:YCR94W(orf name)|uniprotkb:YCR094W(locus name)|uniprotkb:CDC50(gene name)|psi-mi:CDC50(display_short)	psi-mi:atc3_yeast(display_long)|uniprotkb:DRS2(gene name)|psi-mi:DRS2(display_short)|uniprotkb:FUN38(orf name)|uniprotkb:YAL026C(locus name)	psi-mi:"MI:0676"(tandem affinity purification)	Lenoir et al. (2009)	pubmed:19411703|imex:IM-25643	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-13642102|imex:IM-25643-4	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_010018.1|sgd:S000000690|dip:DIP-5138N|ensemblfungi:YCR094W(gene)|ensemblfungi:YCR094W_mRNA(transcript)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005802"(trans-Golgi network)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006897"(endocytosis)|go:"GO:0016021"(integral component of membrane)|go:"GO:0031902"(late endosome membrane)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:0045332"(phospholipid translocation)|go:"GO:0051666"(actin cortical patch localization)|go:"GO:0140331"(aminophospholipid translocation)|go:"GO:1990530"(Cdc50p-Drs2p complex)|interpro:IPR005045(Protein of unknown function DUF284, transmembrane eukaryotic)|rcsb pdb:6PSY|rcsb pdb:6ROH|rcsb pdb:6ROI|rcsb pdb:6ROJ|rcsb pdb:7OH4|rcsb pdb:7OH6|rcsb pdb:7OH7|reactome:R-SCE-6798695|rcsb pdb:6PSX|mint:P25656	refseq:NP_009376.2|sgd:S000000024|dip:DIP-2216N|mint:P39524|ensemblfungi:YAL026C(gene)|ensemblfungi:YAL026C_mRNA(transcript)|go:"GO:0000287"(magnesium ion binding)|go:"GO:0000749"(response to pheromone triggering conjugation with cellular fusion)|go:"GO:0005524"(ATP binding)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005802"(trans-Golgi network)|go:"GO:0005886"(plasma membrane)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006892"(post-Golgi vesicle-mediated transport)|go:"GO:0006897"(endocytosis)|go:"GO:0016021"(integral component of membrane)|go:"GO:0032456"(endocytic recycling)|go:"GO:0045332"(phospholipid translocation)|go:"GO:0090555"(phosphatidylethanolamine flippase activity)|go:"GO:0140326"(ATPase-coupled intramembrane lipid transporter activity)|go:"GO:0140345"(phosphatidylcholine flippase activity)|go:"GO:0140346"(phosphatidylserine flippase activity)|go:"GO:1990530"(Cdc50p-Drs2p complex)|interpro:IPR001757(ATPase, P-type, K/Mg/Cd/Cu/Zn/Na/Ca/Na/H-transporter)|interpro:IPR006539(Phospholipid-translocating P-type ATPase, flippase)|interpro:IPR008250(E1-E2 ATPase-associated region)|interpro:IPR018303|interpro:IPR023214|interpro:IPR023298|interpro:IPR023299|interpro:IPR032630|interpro:IPR032631|interpro:IPR036412|interpro:IPR044492|rcsb pdb:6PSX|rcsb pdb:6PSY|rcsb pdb:6ROH|rcsb pdb:6ROI|rcsb pdb:6ROJ|rcsb pdb:7OH4|rcsb pdb:7OH5|rcsb pdb:7OH6|rcsb pdb:7OH7|reactome:R-SCE-6798695|reactome:R-SCE-936837	-	crc64:565575CA402C0C91	crc64:5745B92901F8E1AE	figure legend:Figs. 2, 4A, 5C, 6A|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:4932(yeasx)|taxid:4932("Saccharomyces cerevisiae (Baker's yeast)")	-	2017/02/06	2017/02/07	rogid:zVi09MNh1hpBUuc5AzasxmCrnKY559292	rogid:qJ8uK7dznIqyjVwjbw/VGn5Bo+4559292	rigid:H/6/aNvfGyK1lAEOYrg5Yd83mgw	false	myc tag:c-c	mutation decreasing interaction strength:560-560|ha tag:n-n|his tag:n-n	-	-	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)
uniprotkb:P25656	uniprotkb:P39524	intact:EBI-22014|uniprotkb:D6VR94|ensemblfungi:YCR094W	intact:EBI-3106|uniprotkb:D6VPJ2|ensemblfungi:YAL026C	psi-mi:cdc50_yeast(display_long)|uniprotkb:YCR94W(orf name)|uniprotkb:YCR094W(locus name)|uniprotkb:CDC50(gene name)|psi-mi:CDC50(display_short)	psi-mi:atc3_yeast(display_long)|uniprotkb:DRS2(gene name)|psi-mi:DRS2(display_short)|uniprotkb:FUN38(orf name)|uniprotkb:YAL026C(locus name)	psi-mi:"MI:0676"(tandem affinity purification)	Lenoir et al. (2009)	pubmed:19411703|imex:IM-25643	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-13642777|imex:IM-25643-8	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_010018.1|sgd:S000000690|dip:DIP-5138N|ensemblfungi:YCR094W(gene)|ensemblfungi:YCR094W_mRNA(transcript)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005802"(trans-Golgi network)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006897"(endocytosis)|go:"GO:0016021"(integral component of membrane)|go:"GO:0031902"(late endosome membrane)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:0045332"(phospholipid translocation)|go:"GO:0051666"(actin cortical patch localization)|go:"GO:0140331"(aminophospholipid translocation)|go:"GO:1990530"(Cdc50p-Drs2p complex)|interpro:IPR005045(Protein of unknown function DUF284, transmembrane eukaryotic)|rcsb pdb:6PSY|rcsb pdb:6ROH|rcsb pdb:6ROI|rcsb pdb:6ROJ|rcsb pdb:7OH4|rcsb pdb:7OH6|rcsb pdb:7OH7|reactome:R-SCE-6798695|rcsb pdb:6PSX|mint:P25656	refseq:NP_009376.2|sgd:S000000024|dip:DIP-2216N|mint:P39524|ensemblfungi:YAL026C(gene)|ensemblfungi:YAL026C_mRNA(transcript)|go:"GO:0000287"(magnesium ion binding)|go:"GO:0000749"(response to pheromone triggering conjugation with cellular fusion)|go:"GO:0005524"(ATP binding)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005802"(trans-Golgi network)|go:"GO:0005886"(plasma membrane)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006892"(post-Golgi vesicle-mediated transport)|go:"GO:0006897"(endocytosis)|go:"GO:0016021"(integral component of membrane)|go:"GO:0032456"(endocytic recycling)|go:"GO:0045332"(phospholipid translocation)|go:"GO:0090555"(phosphatidylethanolamine flippase activity)|go:"GO:0140326"(ATPase-coupled intramembrane lipid transporter activity)|go:"GO:0140345"(phosphatidylcholine flippase activity)|go:"GO:0140346"(phosphatidylserine flippase activity)|go:"GO:1990530"(Cdc50p-Drs2p complex)|interpro:IPR001757(ATPase, P-type, K/Mg/Cd/Cu/Zn/Na/Ca/Na/H-transporter)|interpro:IPR006539(Phospholipid-translocating P-type ATPase, flippase)|interpro:IPR008250(E1-E2 ATPase-associated region)|interpro:IPR018303|interpro:IPR023214|interpro:IPR023298|interpro:IPR023299|interpro:IPR032630|interpro:IPR032631|interpro:IPR036412|interpro:IPR044492|rcsb pdb:6PSX|rcsb pdb:6PSY|rcsb pdb:6ROH|rcsb pdb:6ROI|rcsb pdb:6ROJ|rcsb pdb:7OH4|rcsb pdb:7OH5|rcsb pdb:7OH6|rcsb pdb:7OH7|reactome:R-SCE-6798695|reactome:R-SCE-936837	-	crc64:565575CA402C0C91	crc64:5745B92901F8E1AE	figure legend:Figs 4A, 5D|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:4932(yeasx)|taxid:4932("Saccharomyces cerevisiae (Baker's yeast)")	-	2017/02/06	2017/02/07	rogid:zVi09MNh1hpBUuc5AzasxmCrnKY559292	rogid:qJ8uK7dznIqyjVwjbw/VGn5Bo+4559292	rigid:H/6/aNvfGyK1lAEOYrg5Yd83mgw	false	myc tag:c-c	ha tag:n-n|his tag:n-n	-	-	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)
intenz:3.6.3.1	-	intact:EBI-13642652	-	psi-mi:drs2-cdc50_yeast(display_short)|psi-mi:3.6.3.1(display_long)|intact:DRS2-CDC50 P4-ATPase complex(complex recommended name)|intact:"CDC5:DRS2"(complex systematic name)|intact:Aminophospholipid translocase complex(complex synonym)|intact:APLT complex(complex synonym)|intact:DRS2-CDC50 phospholipid flippase complex(complex synonym)	-	psi-mi:"MI:0841"(phosphotransferase assay)	Lenoir et al. (2009)	pubmed:19411703|imex:IM-25643	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	-	psi-mi:"MI:0844"(phosphotransfer reaction)	psi-mi:"MI:0469"(IntAct)	intact:EBI-13642845|imex:IM-25643-2	-	-	psi-mi:"MI:0898"(putative self)	-	psi-mi:"MI:0898"(putative self)	-	psi-mi:"MI:1302"(stable complex)	-	go:"GO:0005524"(ATP binding)|pubmed:27458383(see-also)|go:"GO:0140326"(ATPase-coupled intramembrane lipid transporter activity)|go:"GO:1990531"(phospholipid-translocating ATPase complex)|go:"GO:0045332"(phospholipid translocation)|intact:EBI-13642102(exp-evidence)|evidence ontology:"ECO:0000353"|complex portal:CPX-1018(complex-primary)	-	-	curated-complex:"A membrane pump which actively translocates, or flips, phospholipids across cell membranes from the exoplasmic to the cytoplasmic leaflet of the lipid bilayer. This generates and maintains membrane lipid asymmetry, a property essential for a wide variety of cellular processes such as vesicle budding and intracellular vesicle trafficking. Transport is accomplished by cyclic changes between two main enzyme conformations, during which the DRS2 ATPase is phosphorylated by ATP at a conserved aspartate residue (Asp-560) and subsequently dephosphorylated. These processes are coupled to vectorial transport and counter-transport by a controlled opening and closing of cytoplasmic and exoplasmic pathways, which give access to the ion-binding sites that are buried inside the membrane-spanning region of the pump"|complex-properties:The membrane  domain of DRS2 serves as the pathway for translocation of lipid substrates across cell membranes and is predicted to contain 10 transmembrane segments. CDC50 is a membrane glycoprotein and appears to contact DRS2 at multiple sites to promote correct protein folding, exit of the P4-ATPase complex from the endoplasmic reticulum, and formation of a functionally active flippase complex.	-	inhibition:"N-ethylmaleimide  (CHEBI:44485) - an inhibitor of the aminophospholipid translocase activity"|inhibition:"Orthovanadate (CHEBI:46442 )"|figure legend:Figs3A-E, 4B, 5E|comment:"The aspartate at position 560 (Asp-560) in Drs2p is part of the DKTGTLT signature sequence and is modified to form the aspartyl-phosphate intermediate. All of the VO4-sensitive phosphorylation was abolished in a asp560asn mutant. This indicates that wild type DRS2 co-purified with CDC50 undergoes VO4-sensitive phosphorylation from ATP at Asp-560."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2017/02/06	2017/02/07	-	-	-	false	calmodulin binding peptide plus protein a tag:n-n|myc tag:c-c	-	-	-	psi-mi:"MI:0821"(molecular weight estimation by autoradiography)	-
uniprotkb:P42838	uniprotkb:P39524	intact:EBI-28396|uniprotkb:D6W0M3|ensemblfungi:YNL323W	intact:EBI-3106|uniprotkb:D6VPJ2|ensemblfungi:YAL026C	psi-mi:lem3_yeast(display_long)|uniprotkb:LEM3(gene name)|psi-mi:LEM3(display_short)|uniprotkb:BRE3(gene name synonym)|uniprotkb:Ro-sensitive 3(gene name synonym)|uniprotkb:Brefeldin-A sensitivity protein 3(gene name synonym)|uniprotkb:ROS3(gene name synonym)|uniprotkb:N0333(orf name)|uniprotkb:YNL323W(locus name)	psi-mi:atc3_yeast(display_long)|uniprotkb:DRS2(gene name)|psi-mi:DRS2(display_short)|uniprotkb:FUN38(orf name)|uniprotkb:YAL026C(locus name)	psi-mi:"MI:0676"(tandem affinity purification)	Lenoir et al. (2009)	pubmed:19411703|imex:IM-25643	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-13642739|imex:IM-25643-6	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_014076.1|sgd:S000005267|dip:DIP-4598N|mint:P42838|ensemblfungi:YNL323W(gene)|ensemblfungi:YNL323W_mRNA(transcript)|go:"GO:0005737"(cytoplasm)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005886"(plasma membrane)|go:"GO:0007166"(cell surface receptor signaling pathway)|go:"GO:0016021"(integral component of membrane)|go:"GO:0044088"(regulation of vacuole organization)|go:"GO:0045332"(phospholipid translocation)|go:"GO:0140331"(aminophospholipid translocation)|go:"GO:1990531"(phospholipid-translocating ATPase complex)|interpro:IPR005045(Protein of unknown function DUF284, transmembrane eukaryotic)|rcsb pdb:7KY5|rcsb pdb:7KY7|rcsb pdb:7KY8|rcsb pdb:7KY9|rcsb pdb:7KYA|rcsb pdb:7KYB|rcsb pdb:7KYC|reactome:R-SCE-6798695	refseq:NP_009376.2|sgd:S000000024|dip:DIP-2216N|mint:P39524|ensemblfungi:YAL026C(gene)|ensemblfungi:YAL026C_mRNA(transcript)|go:"GO:0000287"(magnesium ion binding)|go:"GO:0000749"(response to pheromone triggering conjugation with cellular fusion)|go:"GO:0005524"(ATP binding)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005802"(trans-Golgi network)|go:"GO:0005886"(plasma membrane)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006892"(post-Golgi vesicle-mediated transport)|go:"GO:0006897"(endocytosis)|go:"GO:0016021"(integral component of membrane)|go:"GO:0032456"(endocytic recycling)|go:"GO:0045332"(phospholipid translocation)|go:"GO:0090555"(phosphatidylethanolamine flippase activity)|go:"GO:0140326"(ATPase-coupled intramembrane lipid transporter activity)|go:"GO:0140345"(phosphatidylcholine flippase activity)|go:"GO:0140346"(phosphatidylserine flippase activity)|go:"GO:1990530"(Cdc50p-Drs2p complex)|interpro:IPR001757(ATPase, P-type, K/Mg/Cd/Cu/Zn/Na/Ca/Na/H-transporter)|interpro:IPR006539(Phospholipid-translocating P-type ATPase, flippase)|interpro:IPR008250(E1-E2 ATPase-associated region)|interpro:IPR018303|interpro:IPR023214|interpro:IPR023298|interpro:IPR023299|interpro:IPR032630|interpro:IPR032631|interpro:IPR036412|interpro:IPR044492|rcsb pdb:6PSX|rcsb pdb:6PSY|rcsb pdb:6ROH|rcsb pdb:6ROI|rcsb pdb:6ROJ|rcsb pdb:7OH4|rcsb pdb:7OH5|rcsb pdb:7OH6|rcsb pdb:7OH7|reactome:R-SCE-6798695|reactome:R-SCE-936837	-	crc64:55BDA1F32251B4E6	crc64:5745B92901F8E1AE	figure legend:Fig. 5C|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:4932(yeasx)|taxid:4932("Saccharomyces cerevisiae (Baker's yeast)")	-	2017/02/06	2017/02/07	rogid:YNU4U5F2bWdXZvJYQ9HK0iWX230559292	rogid:qJ8uK7dznIqyjVwjbw/VGn5Bo+4559292	rigid:3IJcpn0yoKcB3SGfrvD03udBhW0	false	myc tag:c-c	ha tag:n-n|his tag:n-n	-	-	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)
uniprotkb:P42838	uniprotkb:P32660	intact:EBI-28396|uniprotkb:D6W0M3|ensemblfungi:YNL323W	intact:EBI-3121|uniprotkb:D3DM74|ensemblfungi:YER166W	psi-mi:lem3_yeast(display_long)|uniprotkb:LEM3(gene name)|psi-mi:LEM3(display_short)|uniprotkb:BRE3(gene name synonym)|uniprotkb:Ro-sensitive 3(gene name synonym)|uniprotkb:Brefeldin-A sensitivity protein 3(gene name synonym)|uniprotkb:ROS3(gene name synonym)|uniprotkb:N0333(orf name)|uniprotkb:YNL323W(locus name)	psi-mi:atc5_yeast(display_long)|uniprotkb:DNF1(gene name)|psi-mi:DNF1(display_short)|uniprotkb:YER166W(locus name)|uniprotkb:SYGP-ORF7(orf name)|uniprotkb:Flippase DNF1(gene name synonym)	psi-mi:"MI:0112"(ubiquitin reconstruction)	Lenoir et al. (2009)	pubmed:19411703|imex:IM-25643	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-13642887|imex:IM-25643-10	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_014076.1|sgd:S000005267|dip:DIP-4598N|mint:P42838|ensemblfungi:YNL323W(gene)|ensemblfungi:YNL323W_mRNA(transcript)|go:"GO:0005737"(cytoplasm)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005886"(plasma membrane)|go:"GO:0007166"(cell surface receptor signaling pathway)|go:"GO:0016021"(integral component of membrane)|go:"GO:0044088"(regulation of vacuole organization)|go:"GO:0045332"(phospholipid translocation)|go:"GO:0140331"(aminophospholipid translocation)|go:"GO:1990531"(phospholipid-translocating ATPase complex)|interpro:IPR005045(Protein of unknown function DUF284, transmembrane eukaryotic)|rcsb pdb:7KY5|rcsb pdb:7KY7|rcsb pdb:7KY8|rcsb pdb:7KY9|rcsb pdb:7KYA|rcsb pdb:7KYB|rcsb pdb:7KYC|reactome:R-SCE-6798695	sgd:S000000968|go:"GO:0045332"(phospholipid translocation)|go:"GO:0070867"(mating projection tip membrane)|go:"GO:0071944"(cell periphery)|go:"GO:0090555"(phosphatidylethanolamine flippase activity)|go:"GO:0140326"(ATPase-coupled intramembrane lipid transporter activity)|go:"GO:0140345"(phosphatidylcholine flippase activity)|go:"GO:1990531"(phospholipid-translocating ATPase complex)|interpro:IPR001757(ATPase, P-type, K/Mg/Cd/Cu/Zn/Na/Ca/Na/H-transporter)|interpro:IPR006539(Phospholipid-translocating P-type ATPase, flippase)|interpro:IPR008250(E1-E2 ATPase-associated region)|interpro:IPR018303|interpro:IPR023214|interpro:IPR023298|interpro:IPR023299|interpro:IPR026871|interpro:IPR032630|interpro:IPR032631|interpro:IPR036412|interpro:IPR044492|rcsb pdb:7KY6|rcsb pdb:7KYB|rcsb pdb:7KYC|refseq:NP_011093.3|dip:DIP-7949N|mint:P32660|ensemblfungi:YER166W(gene)|ensemblfungi:YER166W_mRNA(transcript)|go:"GO:0000287"(magnesium ion binding)|go:"GO:0000749"(response to pheromone triggering conjugation with cellular fusion)|go:"GO:0005524"(ATP binding)|go:"GO:0005739"(mitochondrion)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005886"(plasma membrane)|go:"GO:0005935"(cellular bud neck)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006897"(endocytosis)|go:"GO:0007163"(establishment or maintenance of cell polarity)|go:"GO:0010008"(endosome membrane)|go:"GO:0016021"(integral component of membrane)|go:"GO:0042802"(identical protein binding)	-	crc64:55BDA1F32251B4E6	crc64:3CC3FBA8ADDE8960	figure legend:Figs. 6 B/E|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:4932(yeasx)|taxid:4932("Saccharomyces cerevisiae (Baker's yeast)")	-	2017/02/06	2017/02/07	rogid:YNU4U5F2bWdXZvJYQ9HK0iWX230559292	rogid:4ciEaV0aGjDqtpexkFJ0l737SpA559292	rigid:K113XbAj8d/ppcLMEzF12rkjy14	false	nub:n-n	cub:c-c|mutation disrupting interaction strength:667-667|mutation disrupting interaction strength:667-667	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
uniprotkb:P53740	uniprotkb:Q12674	intact:EBI-28524|uniprotkb:D6W1M3|ensemblfungi:YNR048W	intact:EBI-3142|uniprotkb:D6VZY4|ensemblfungi:YMR162C	psi-mi:yn8s_yeast(display_long)|uniprotkb:CDC50/ROS3 family protein 1(gene name synonym)|uniprotkb:N3453(orf name)|uniprotkb:YNR048W(locus name)	psi-mi:atc8_yeast(display_long)|uniprotkb:DNF3(gene name)|psi-mi:DNF3(display_short)|uniprotkb:Aminophospholipid translocase(gene name synonym)|uniprotkb:Phospholipid translocase(gene name synonym)|uniprotkb:YM8520.11C(orf name)|uniprotkb:YMR162C(locus name)	psi-mi:"MI:0112"(ubiquitin reconstruction)	Lenoir et al. (2009)	pubmed:19411703|imex:IM-25643	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-13642915|imex:IM-25643-12	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	sgd:S000005331|refseq:NP_014446.3|dip:DIP-1250N|reactome:R-SCE-6798695|ensemblfungi:YNR048W(gene)|ensemblfungi:YNR048W_mRNA(transcript)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005802"(trans-Golgi network)|go:"GO:0005886"(plasma membrane)|go:"GO:0016021"(integral component of membrane)|go:"GO:0045332"(phospholipid translocation)|interpro:IPR005045(Protein of unknown function DUF284, transmembrane eukaryotic)	refseq:NP_013885.1|sgd:S000004772|interpro:IPR008250(E1-E2 ATPase-associated region)|interpro:IPR023299|interpro:IPR001757(ATPase, P-type, K/Mg/Cd/Cu/Zn/Na/Ca/Na/H-transporter)|interpro:IPR018303|interpro:IPR006539(Phospholipid-translocating P-type ATPase, flippase)|interpro:IPR023214|interpro:IPR032630|interpro:IPR032631|interpro:IPR023298|interpro:IPR036412|ensemblfungi:YMR162C(gene)|ensemblfungi:YMR162C_mRNA(transcript)|go:"GO:0000287"(magnesium ion binding)|go:"GO:0000749"(response to pheromone triggering conjugation with cellular fusion)|reactome:R-SCE-6798695|reactome:R-SCE-936837|go:"GO:0005524"(ATP binding)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005802"(trans-Golgi network)|go:"GO:0005886"(plasma membrane)|go:"GO:0016021"(integral component of membrane)|go:"GO:0030140"(trans-Golgi network transport vesicle)|go:"GO:0045332"(phospholipid translocation)|go:"GO:0070867"(mating projection tip membrane)|go:"GO:0090555"(phosphatidylethanolamine flippase activity)|go:"GO:0140326"(ATPase-coupled intramembrane lipid transporter activity)|go:"GO:0140345"(phosphatidylcholine flippase activity)|go:"GO:0140346"(phosphatidylserine flippase activity)	-	-	-	figure legend:Figs. 6B|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:4932(yeasx)|taxid:4932("Saccharomyces cerevisiae (Baker's yeast)")	-	2017/02/06	2017/02/22	rogid:MncHVMyjKiasqdyZuY2AXKeYuOE559292	rogid:ps8epWs4G79x44mlIQyoRIfpjFU559292	rigid:jLKsnZAj792qqvj86vo7KJnwMgA	false	nub:n-n	cub:c-c	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
uniprotkb:P25656	uniprotkb:P39524	intact:EBI-22014|uniprotkb:D6VR94|ensemblfungi:YCR094W	intact:EBI-3106|uniprotkb:D6VPJ2|ensemblfungi:YAL026C	psi-mi:cdc50_yeast(display_long)|uniprotkb:YCR94W(orf name)|uniprotkb:YCR094W(locus name)|uniprotkb:CDC50(gene name)|psi-mi:CDC50(display_short)	psi-mi:atc3_yeast(display_long)|uniprotkb:DRS2(gene name)|psi-mi:DRS2(display_short)|uniprotkb:FUN38(orf name)|uniprotkb:YAL026C(locus name)	psi-mi:"MI:0112"(ubiquitin reconstruction)	Lenoir et al. (2009)	pubmed:19411703|imex:IM-25643	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-13642941|imex:IM-25643-14	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_010018.1|sgd:S000000690|dip:DIP-5138N|ensemblfungi:YCR094W(gene)|ensemblfungi:YCR094W_mRNA(transcript)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005802"(trans-Golgi network)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006897"(endocytosis)|go:"GO:0016021"(integral component of membrane)|go:"GO:0031902"(late endosome membrane)|go:"GO:0042147"(retrograde transport, endosome to Golgi)|go:"GO:0045332"(phospholipid translocation)|go:"GO:0051666"(actin cortical patch localization)|go:"GO:0140331"(aminophospholipid translocation)|go:"GO:1990530"(Cdc50p-Drs2p complex)|interpro:IPR005045(Protein of unknown function DUF284, transmembrane eukaryotic)|rcsb pdb:6PSY|rcsb pdb:6ROH|rcsb pdb:6ROI|rcsb pdb:6ROJ|rcsb pdb:7OH4|rcsb pdb:7OH6|rcsb pdb:7OH7|reactome:R-SCE-6798695|rcsb pdb:6PSX|mint:P25656	refseq:NP_009376.2|sgd:S000000024|dip:DIP-2216N|mint:P39524|ensemblfungi:YAL026C(gene)|ensemblfungi:YAL026C_mRNA(transcript)|go:"GO:0000287"(magnesium ion binding)|go:"GO:0000749"(response to pheromone triggering conjugation with cellular fusion)|go:"GO:0005524"(ATP binding)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005802"(trans-Golgi network)|go:"GO:0005886"(plasma membrane)|go:"GO:0006886"(intracellular protein transport)|go:"GO:0006892"(post-Golgi vesicle-mediated transport)|go:"GO:0006897"(endocytosis)|go:"GO:0016021"(integral component of membrane)|go:"GO:0032456"(endocytic recycling)|go:"GO:0045332"(phospholipid translocation)|go:"GO:0090555"(phosphatidylethanolamine flippase activity)|go:"GO:0140326"(ATPase-coupled intramembrane lipid transporter activity)|go:"GO:0140345"(phosphatidylcholine flippase activity)|go:"GO:0140346"(phosphatidylserine flippase activity)|go:"GO:1990530"(Cdc50p-Drs2p complex)|interpro:IPR001757(ATPase, P-type, K/Mg/Cd/Cu/Zn/Na/Ca/Na/H-transporter)|interpro:IPR006539(Phospholipid-translocating P-type ATPase, flippase)|interpro:IPR008250(E1-E2 ATPase-associated region)|interpro:IPR018303|interpro:IPR023214|interpro:IPR023298|interpro:IPR023299|interpro:IPR032630|interpro:IPR032631|interpro:IPR036412|interpro:IPR044492|rcsb pdb:6PSX|rcsb pdb:6PSY|rcsb pdb:6ROH|rcsb pdb:6ROI|rcsb pdb:6ROJ|rcsb pdb:7OH4|rcsb pdb:7OH5|rcsb pdb:7OH6|rcsb pdb:7OH7|reactome:R-SCE-6798695|reactome:R-SCE-936837	-	crc64:565575CA402C0C91	crc64:5745B92901F8E1AE	figure legend:Figs. 6B-D, 7B-D|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:4932(yeasx)|taxid:4932("Saccharomyces cerevisiae (Baker's yeast)")	-	2017/02/06	2017/02/07	rogid:zVi09MNh1hpBUuc5AzasxmCrnKY559292	rogid:qJ8uK7dznIqyjVwjbw/VGn5Bo+4559292	rigid:H/6/aNvfGyK1lAEOYrg5Yd83mgw	false	nub:n-n	mutation with no effect:342-342|mutation decreasing interaction strength:341-341|mutation decreasing interaction strength:560-560|mutation decreasing interaction strength:560-560|cub:c-c	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
chebi:"CHEBI:15422"	intenz:3.6.3.1	intact:EBI-1108845|chebi:"CHEBI:40938"|chebi:"CHEBI:10841"|chebi:"CHEBI:10789"|chebi:"CHEBI:2359"|chebi:"CHEBI:13236"|chebi:"CHEBI:22249"	intact:EBI-13642652	psi-mi:atp(display_short)|psi-mi:"CHEBI:15422"(display_long)|intact:Adenosine 5'-triphosphate(synonym)|intact:Adenosine triphosphate(synonym)|intact:ADENOSINE-5'-TRIPHOSPHATE(synonym)|intact:Adephos(synonym)|intact:Adetol(synonym)|intact:Adynol(synonym)|intact:Atipi(synonym)|intact:ATP(synonym)|intact:Atriphos(synonym)|intact:Cardenosine(synonym)|intact:Fosfobion(synonym)|intact:Glucobasin(synonym)|intact:H4atp(synonym)|intact:Myotriphos(synonym)|intact:Triadenyl(synonym)|intact:Triphosphaden(synonym)|intact:"adenosine 5'-(tetrahydrogen triphosphate)"(iupac name)	psi-mi:drs2-cdc50_yeast(display_short)|psi-mi:3.6.3.1(display_long)|intact:DRS2-CDC50 P4-ATPase complex(complex recommended name)|intact:"CDC5:DRS2"(complex systematic name)|intact:Aminophospholipid translocase complex(complex synonym)|intact:APLT complex(complex synonym)|intact:DRS2-CDC50 phospholipid flippase complex(complex synonym)	psi-mi:"MI:0880"(atpase assay)	Lenoir et al. (2009)	pubmed:19411703|imex:IM-25643	taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)")	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	psi-mi:"MI:0882"(atpase reaction)	psi-mi:"MI:0469"(IntAct)	intact:EBI-13643002|imex:IM-25643-3	-	-	psi-mi:"MI:0502"(enzyme target)	psi-mi:"MI:0501"(enzyme)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0328"(small molecule)	psi-mi:"MI:1302"(stable complex)	-	go:"GO:0005524"(ATP binding)|pubmed:27458383(see-also)|go:"GO:0140326"(ATPase-coupled intramembrane lipid transporter activity)|go:"GO:1990531"(phospholipid-translocating ATPase complex)|go:"GO:0045332"(phospholipid translocation)|intact:EBI-13642102(exp-evidence)|evidence ontology:"ECO:0000353"|complex portal:CPX-1018(complex-primary)	go:"GO:0016887"(ATP hydrolysis activity)	-	curated-complex:"A membrane pump which actively translocates, or flips, phospholipids across cell membranes from the exoplasmic to the cytoplasmic leaflet of the lipid bilayer. This generates and maintains membrane lipid asymmetry, a property essential for a wide variety of cellular processes such as vesicle budding and intracellular vesicle trafficking. Transport is accomplished by cyclic changes between two main enzyme conformations, during which the DRS2 ATPase is phosphorylated by ATP at a conserved aspartate residue (Asp-560) and subsequently dephosphorylated. These processes are coupled to vectorial transport and counter-transport by a controlled opening and closing of cytoplasmic and exoplasmic pathways, which give access to the ion-binding sites that are buried inside the membrane-spanning region of the pump"|complex-properties:The membrane  domain of DRS2 serves as the pathway for translocation of lipid substrates across cell membranes and is predicted to contain 10 transmembrane segments. CDC50 is a membrane glycoprotein and appears to contact DRS2 at multiple sites to promote correct protein folding, exit of the P4-ATPase complex from the endoplasmic reticulum, and formation of a functionally active flippase complex.	inhibition:"Orthovanadate (CHEBI:46442 )"|figure legend:Supp Fig 2|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2017/02/06	2017/02/07	-	-	-	false	-	calmodulin binding peptide plus protein a tag:n-n|myc tag:c-c	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)