#ID(s) interactor A	ID(s) interactor B	Alt. ID(s) interactor A	Alt. ID(s) interactor B	Alias(es) interactor A	Alias(es) interactor B	Interaction detection method(s)	Publication 1st author(s)	Publication Identifier(s)	Taxid interactor A	Taxid interactor B	Interaction type(s)	Source database(s)	Interaction identifier(s)	Confidence value(s)	Expansion method(s)	Biological role(s) interactor A	Biological role(s) interactor B	Experimental role(s) interactor A	Experimental role(s) interactor B	Type(s) interactor A	Type(s) interactor B	Xref(s) interactor A	Xref(s) interactor B	Interaction Xref(s)	Annotation(s) interactor A	Annotation(s) interactor B	Interaction annotation(s)	Host organism(s)	Interaction parameter(s)	Creation date	Update date	Checksum(s) interactor A	Checksum(s) interactor B	Interaction Checksum(s)	Negative	Feature(s) interactor A	Feature(s) interactor B	Stoichiometry(s) interactor A	Stoichiometry(s) interactor B	Identification method participant A	Identification method participant B
uniprotkb:P10844	uniprotkb:P29101	intact:EBI-7661991|uniprotkb:P10843|intact:MINT-1795550	intact:EBI-458017	psi-mi:bxb_clobo(display_long)|uniprotkb:botB(gene name)|psi-mi:botB(display_short)|uniprotkb:Bontoxilysin-B(gene name synonym)	psi-mi:syt2_rat(display_long)|uniprotkb:Syt2(gene name)|psi-mi:Syt2(display_short)|uniprotkb:Synaptotagmin II(gene name synonym)	psi-mi:"MI:0065"(isothermal titration calorimetry)	Jin et al. (2006)	pubmed:17167421|doi:10.1038/nature05387|imex:IM-21619	taxid:1491(clobo)|taxid:1491(Clostridium botulinum)	taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)")	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0465"(DIP)	intact:EBI-15615934|imex:IM-21619-1|dip:DIP-72743E	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	go:"GO:0004222"(metalloendopeptidase activity)|go:"GO:0005576"(extracellular region)|go:"GO:0008270"(zinc ion binding)|go:"GO:0008289"(lipid binding)|go:"GO:0008320"(protein transmembrane transporter activity)|go:"GO:0016021"(integral component of membrane)|go:"GO:0044156"(host cell junction)|go:"GO:0044161"(host cell cytoplasmic vesicle)|go:"GO:0044164"(host cell cytosol)|go:"GO:0044231"(host cell presynaptic membrane)|go:"GO:0046929"(negative regulation of neurotransmitter secretion)|go:"GO:0090729"(toxin activity)|interpro:IPR000395(Peptidase M27, bontoxilysin)|interpro:IPR011065(Kunitz inhibitor ST1-like)|interpro:IPR012500(Clostridium neurotoxin, translocation)|interpro:IPR012928(Clostridium neurotoxin, receptor binding N-terminal)|interpro:IPR013104(Clostridium neurotoxin, receptor-binding C-terminal)|interpro:IPR013320(Concanavalin A-like lectin/glucanase, subgroup)|interpro:IPR036248|rcsb pdb:1EPW|rcsb pdb:1F31|rcsb pdb:1F82|rcsb pdb:1G9A|rcsb pdb:1G9B|rcsb pdb:1G9C|rcsb pdb:1G9D|rcsb pdb:1I1E|rcsb pdb:1S0B|rcsb pdb:1S0C|rcsb pdb:1S0D|rcsb pdb:1S0E|rcsb pdb:1S0F|rcsb pdb:1S0G|rcsb pdb:1Z0H|rcsb pdb:2ETF|rcsb pdb:2NM1|rcsb pdb:2NP0|rcsb pdb:2XHL|rcsb pdb:3ZUQ|rcsb pdb:4KBB|rcsb pdb:5VID|rcsb pdb:5VMR|rcsb pdb:6G5F|rcsb pdb:6G5G|rcsb pdb:6G5K|rcsb pdb:6QNS|rcsb pdb:6UC6|rcsb pdb:6UFT|rcsb pdb:6UHT|rcsb pdb:6UL4|refseq:WP_012291519.1|reactome:R-HSA-5250958|dip:DIP-42782N	rgd:3804|refseq:NP_036797.1|go:"GO:0000149"(SNARE binding)|go:"GO:0001786"(phosphatidylserine binding)|go:"GO:0005509"(calcium ion binding)|go:"GO:0005544"(calcium-dependent phospholipid binding)|go:"GO:0005886"(plasma membrane)|go:"GO:0014059"(regulation of dopamine secretion)|go:"GO:0016079"(synaptic vesicle exocytosis)|go:"GO:0016192"(vesicle-mediated transport)|go:"GO:0017156"(calcium-ion regulated exocytosis)|go:"GO:0017158"(regulation of calcium ion-dependent exocytosis)|go:"GO:0019905"(syntaxin binding)|go:"GO:0030154"(cell differentiation)|go:"GO:0030276"(clathrin binding)|go:"GO:0030285"(integral component of synaptic vesicle membrane)|go:"GO:0030424"(axon)|go:"GO:0030672"(synaptic vesicle membrane)|interpro:IPR015428(Synaptotagmin 1,2)|interpro:IPR035892|rcsb pdb:2NM1|rcsb pdb:4ISR|go:"GO:0031045"(dense core granule)|reactome:R-RNO-8856825|reactome:R-RNO-8856828|go:"GO:0031594"(neuromuscular junction)|go:"GO:0042584"(chromaffin granule membrane)|go:"GO:0043195"(terminal bouton)|go:"GO:0043533"(inositol 1,3,4,5 tetrakisphosphate binding)|go:"GO:0048488"(synaptic vesicle endocytosis)|go:"GO:0048791"(calcium ion-regulated exocytosis of neurotransmitter)|go:"GO:0070382"(exocytic vesicle)|go:"GO:0071277"(cellular response to calcium ion)|go:"GO:0099502"(calcium-dependent activation of synaptic vesicle fusion)|go:"GO:1903861"(positive regulation of dendrite extension)|interpro:IPR000008(C2 calcium-dependent membrane targeting)|interpro:IPR001565(Synaptotagmin)|dip:DIP-32642N|mint:P29101	psi-mi:"MI:0465"(dip)	comment:mint|function:"Botulinum toxin acts by inhibiting neurotransmitter release. It binds to peripheral neuronal synapses, is internalized and moves by retrograde transport up the axon into the spinal cord where it can move between postsynaptic and presynaptic neurons. It inhibits neurotransmitter release by acting as a zinc endopeptidase that cleaves the '76-Gln-|-Phe-77' bond of synaptobrevin-2"	-	full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2017/08/05	2017/08/10	rogid:TyP1/JJzdgNBsIqolX4P5Vc+9Ag1491	rogid:8gq8JFiYjNR21IjwMvs4cxgKIL010116	rigid:5VTvp7jz8LSWDObE8OcmI6JyJJQ	false	sufficient binding region:858-1291	sufficient binding region:1-61	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
uniprotkb:P10844	uniprotkb:P29101	intact:EBI-7661991|uniprotkb:P10843|intact:MINT-1795550	intact:EBI-458017	psi-mi:bxb_clobo(display_long)|uniprotkb:botB(gene name)|psi-mi:botB(display_short)|uniprotkb:Bontoxilysin-B(gene name synonym)	psi-mi:syt2_rat(display_long)|uniprotkb:Syt2(gene name)|psi-mi:Syt2(display_short)|uniprotkb:Synaptotagmin II(gene name synonym)	psi-mi:"MI:0096"(pull down)	Jin et al. (2006)	pubmed:17167421|doi:10.1038/nature05387|imex:IM-21619	taxid:1491(clobo)|taxid:1491(Clostridium botulinum)	taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)")	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0465"(DIP)	intact:EBI-15615955|imex:IM-21619-2|dip:DIP-72743E	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	go:"GO:0004222"(metalloendopeptidase activity)|go:"GO:0005576"(extracellular region)|go:"GO:0008270"(zinc ion binding)|go:"GO:0008289"(lipid binding)|go:"GO:0008320"(protein transmembrane transporter activity)|go:"GO:0016021"(integral component of membrane)|go:"GO:0044156"(host cell junction)|go:"GO:0044161"(host cell cytoplasmic vesicle)|go:"GO:0044164"(host cell cytosol)|go:"GO:0044231"(host cell presynaptic membrane)|go:"GO:0046929"(negative regulation of neurotransmitter secretion)|go:"GO:0090729"(toxin activity)|interpro:IPR000395(Peptidase M27, bontoxilysin)|interpro:IPR011065(Kunitz inhibitor ST1-like)|interpro:IPR012500(Clostridium neurotoxin, translocation)|interpro:IPR012928(Clostridium neurotoxin, receptor binding N-terminal)|interpro:IPR013104(Clostridium neurotoxin, receptor-binding C-terminal)|interpro:IPR013320(Concanavalin A-like lectin/glucanase, subgroup)|interpro:IPR036248|rcsb pdb:1EPW|rcsb pdb:1F31|rcsb pdb:1F82|rcsb pdb:1G9A|rcsb pdb:1G9B|rcsb pdb:1G9C|rcsb pdb:1G9D|rcsb pdb:1I1E|rcsb pdb:1S0B|rcsb pdb:1S0C|rcsb pdb:1S0D|rcsb pdb:1S0E|rcsb pdb:1S0F|rcsb pdb:1S0G|rcsb pdb:1Z0H|rcsb pdb:2ETF|rcsb pdb:2NM1|rcsb pdb:2NP0|rcsb pdb:2XHL|rcsb pdb:3ZUQ|rcsb pdb:4KBB|rcsb pdb:5VID|rcsb pdb:5VMR|rcsb pdb:6G5F|rcsb pdb:6G5G|rcsb pdb:6G5K|rcsb pdb:6QNS|rcsb pdb:6UC6|rcsb pdb:6UFT|rcsb pdb:6UHT|rcsb pdb:6UL4|refseq:WP_012291519.1|reactome:R-HSA-5250958|dip:DIP-42782N	rgd:3804|refseq:NP_036797.1|go:"GO:0000149"(SNARE binding)|go:"GO:0001786"(phosphatidylserine binding)|go:"GO:0005509"(calcium ion binding)|go:"GO:0005544"(calcium-dependent phospholipid binding)|go:"GO:0005886"(plasma membrane)|go:"GO:0014059"(regulation of dopamine secretion)|go:"GO:0016079"(synaptic vesicle exocytosis)|go:"GO:0016192"(vesicle-mediated transport)|go:"GO:0017156"(calcium-ion regulated exocytosis)|go:"GO:0017158"(regulation of calcium ion-dependent exocytosis)|go:"GO:0019905"(syntaxin binding)|go:"GO:0030154"(cell differentiation)|go:"GO:0030276"(clathrin binding)|go:"GO:0030285"(integral component of synaptic vesicle membrane)|go:"GO:0030424"(axon)|go:"GO:0030672"(synaptic vesicle membrane)|interpro:IPR015428(Synaptotagmin 1,2)|interpro:IPR035892|rcsb pdb:2NM1|rcsb pdb:4ISR|go:"GO:0031045"(dense core granule)|reactome:R-RNO-8856825|reactome:R-RNO-8856828|go:"GO:0031594"(neuromuscular junction)|go:"GO:0042584"(chromaffin granule membrane)|go:"GO:0043195"(terminal bouton)|go:"GO:0043533"(inositol 1,3,4,5 tetrakisphosphate binding)|go:"GO:0048488"(synaptic vesicle endocytosis)|go:"GO:0048791"(calcium ion-regulated exocytosis of neurotransmitter)|go:"GO:0070382"(exocytic vesicle)|go:"GO:0071277"(cellular response to calcium ion)|go:"GO:0099502"(calcium-dependent activation of synaptic vesicle fusion)|go:"GO:1903861"(positive regulation of dendrite extension)|interpro:IPR000008(C2 calcium-dependent membrane targeting)|interpro:IPR001565(Synaptotagmin)|dip:DIP-32642N|mint:P29101	psi-mi:"MI:0465"(dip)	comment:mint|function:"Botulinum toxin acts by inhibiting neurotransmitter release. It binds to peripheral neuronal synapses, is internalized and moves by retrograde transport up the axon into the spinal cord where it can move between postsynaptic and presynaptic neurons. It inhibits neurotransmitter release by acting as a zinc endopeptidase that cleaves the '76-Gln-|-Phe-77' bond of synaptobrevin-2"	-	full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2017/08/05	2017/08/10	rogid:TyP1/JJzdgNBsIqolX4P5Vc+9Ag1491	rogid:8gq8JFiYjNR21IjwMvs4cxgKIL010116	rigid:5VTvp7jz8LSWDObE8OcmI6JyJJQ	false	sufficient binding region:858-1291|mutation decreasing interaction strength:1118-1118|mutation decreasing interaction strength:1192-1192|mutation disrupting interaction strength:1194-1194|mutation disrupting interaction strength:1196-1196|mutation disrupting interaction strength:1204-1204	glutathione s tranferase tag:n-n|sufficient binding region:1-61	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
uniprotkb:P10844	uniprotkb:P29101	intact:EBI-7661991|uniprotkb:P10843|intact:MINT-1795550	intact:EBI-458017	psi-mi:bxb_clobo(display_long)|uniprotkb:botB(gene name)|psi-mi:botB(display_short)|uniprotkb:Bontoxilysin-B(gene name synonym)	psi-mi:syt2_rat(display_long)|uniprotkb:Syt2(gene name)|psi-mi:Syt2(display_short)|uniprotkb:Synaptotagmin II(gene name synonym)	psi-mi:"MI:0096"(pull down)	Jin et al. (2006)	pubmed:17167421|doi:10.1038/nature05387|imex:IM-21619	taxid:1491(clobo)|taxid:1491(Clostridium botulinum)	taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)")	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0465"(DIP)	intact:EBI-15615992|imex:IM-21619-3|dip:DIP-72743E	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	go:"GO:0004222"(metalloendopeptidase activity)|go:"GO:0005576"(extracellular region)|go:"GO:0008270"(zinc ion binding)|go:"GO:0008289"(lipid binding)|go:"GO:0008320"(protein transmembrane transporter activity)|go:"GO:0016021"(integral component of membrane)|go:"GO:0044156"(host cell junction)|go:"GO:0044161"(host cell cytoplasmic vesicle)|go:"GO:0044164"(host cell cytosol)|go:"GO:0044231"(host cell presynaptic membrane)|go:"GO:0046929"(negative regulation of neurotransmitter secretion)|go:"GO:0090729"(toxin activity)|interpro:IPR000395(Peptidase M27, bontoxilysin)|interpro:IPR011065(Kunitz inhibitor ST1-like)|interpro:IPR012500(Clostridium neurotoxin, translocation)|interpro:IPR012928(Clostridium neurotoxin, receptor binding N-terminal)|interpro:IPR013104(Clostridium neurotoxin, receptor-binding C-terminal)|interpro:IPR013320(Concanavalin A-like lectin/glucanase, subgroup)|interpro:IPR036248|rcsb pdb:1EPW|rcsb pdb:1F31|rcsb pdb:1F82|rcsb pdb:1G9A|rcsb pdb:1G9B|rcsb pdb:1G9C|rcsb pdb:1G9D|rcsb pdb:1I1E|rcsb pdb:1S0B|rcsb pdb:1S0C|rcsb pdb:1S0D|rcsb pdb:1S0E|rcsb pdb:1S0F|rcsb pdb:1S0G|rcsb pdb:1Z0H|rcsb pdb:2ETF|rcsb pdb:2NM1|rcsb pdb:2NP0|rcsb pdb:2XHL|rcsb pdb:3ZUQ|rcsb pdb:4KBB|rcsb pdb:5VID|rcsb pdb:5VMR|rcsb pdb:6G5F|rcsb pdb:6G5G|rcsb pdb:6G5K|rcsb pdb:6QNS|rcsb pdb:6UC6|rcsb pdb:6UFT|rcsb pdb:6UHT|rcsb pdb:6UL4|refseq:WP_012291519.1|reactome:R-HSA-5250958|dip:DIP-42782N	rgd:3804|refseq:NP_036797.1|go:"GO:0000149"(SNARE binding)|go:"GO:0001786"(phosphatidylserine binding)|go:"GO:0005509"(calcium ion binding)|go:"GO:0005544"(calcium-dependent phospholipid binding)|go:"GO:0005886"(plasma membrane)|go:"GO:0014059"(regulation of dopamine secretion)|go:"GO:0016079"(synaptic vesicle exocytosis)|go:"GO:0016192"(vesicle-mediated transport)|go:"GO:0017156"(calcium-ion regulated exocytosis)|go:"GO:0017158"(regulation of calcium ion-dependent exocytosis)|go:"GO:0019905"(syntaxin binding)|go:"GO:0030154"(cell differentiation)|go:"GO:0030276"(clathrin binding)|go:"GO:0030285"(integral component of synaptic vesicle membrane)|go:"GO:0030424"(axon)|go:"GO:0030672"(synaptic vesicle membrane)|interpro:IPR015428(Synaptotagmin 1,2)|interpro:IPR035892|rcsb pdb:2NM1|rcsb pdb:4ISR|go:"GO:0031045"(dense core granule)|reactome:R-RNO-8856825|reactome:R-RNO-8856828|go:"GO:0031594"(neuromuscular junction)|go:"GO:0042584"(chromaffin granule membrane)|go:"GO:0043195"(terminal bouton)|go:"GO:0043533"(inositol 1,3,4,5 tetrakisphosphate binding)|go:"GO:0048488"(synaptic vesicle endocytosis)|go:"GO:0048791"(calcium ion-regulated exocytosis of neurotransmitter)|go:"GO:0070382"(exocytic vesicle)|go:"GO:0071277"(cellular response to calcium ion)|go:"GO:0099502"(calcium-dependent activation of synaptic vesicle fusion)|go:"GO:1903861"(positive regulation of dendrite extension)|interpro:IPR000008(C2 calcium-dependent membrane targeting)|interpro:IPR001565(Synaptotagmin)|dip:DIP-32642N|mint:P29101	psi-mi:"MI:0465"(dip)	comment:mint|function:"Botulinum toxin acts by inhibiting neurotransmitter release. It binds to peripheral neuronal synapses, is internalized and moves by retrograde transport up the axon into the spinal cord where it can move between postsynaptic and presynaptic neurons. It inhibits neurotransmitter release by acting as a zinc endopeptidase that cleaves the '76-Gln-|-Phe-77' bond of synaptobrevin-2"	-	full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2017/08/05	2017/08/10	rogid:TyP1/JJzdgNBsIqolX4P5Vc+9Ag1491	rogid:8gq8JFiYjNR21IjwMvs4cxgKIL010116	rigid:5VTvp7jz8LSWDObE8OcmI6JyJJQ	false	sufficient binding region:858-1291	sufficient binding region:1-61|glutathione s tranferase tag:n-n|mutation disrupting interaction strength:47-47|mutation disrupting interaction strength:54-54|mutation disrupting interaction strength:55-55|mutation disrupting interaction strength:57-57|mutation decreasing interaction strength:50-50	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)