#ID(s) interactor A	ID(s) interactor B	Alt. ID(s) interactor A	Alt. ID(s) interactor B	Alias(es) interactor A	Alias(es) interactor B	Interaction detection method(s)	Publication 1st author(s)	Publication Identifier(s)	Taxid interactor A	Taxid interactor B	Interaction type(s)	Source database(s)	Interaction identifier(s)	Confidence value(s)	Expansion method(s)	Biological role(s) interactor A	Biological role(s) interactor B	Experimental role(s) interactor A	Experimental role(s) interactor B	Type(s) interactor A	Type(s) interactor B	Xref(s) interactor A	Xref(s) interactor B	Interaction Xref(s)	Annotation(s) interactor A	Annotation(s) interactor B	Interaction annotation(s)	Host organism(s)	Interaction parameter(s)	Creation date	Update date	Checksum(s) interactor A	Checksum(s) interactor B	Interaction Checksum(s)	Negative	Feature(s) interactor A	Feature(s) interactor B	Stoichiometry(s) interactor A	Stoichiometry(s) interactor B	Identification method participant A	Identification method participant B
uniprotkb:P32481	uniprotkb:P20459	intact:EBI-8924|ensemblfungi:YER025W|uniprotkb:D3DLS4	intact:EBI-8915|uniprotkb:E9P8T6|ensemblfungi:YJR007W|uniprotkb:D6VWI2	psi-mi:if2g_yeast(display_long)|uniprotkb:YER025W(locus name)|uniprotkb:GCD11(gene name)|psi-mi:GCD11(display_short)|uniprotkb:TIF213(gene name synonym)|uniprotkb:SUI4(gene name synonym)	psi-mi:if2a_yeast(display_long)|uniprotkb:SUI2(gene name)|psi-mi:SUI2(display_short)|uniprotkb:TIF211(gene name synonym)|uniprotkb:YJR007W(locus name)|uniprotkb:J1429(orf name)	psi-mi:"MI:0091"(chromatography technology)	Erickson et al. (1996)	pubmed:8947054|imex:IM-25295	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-11710491|imex:IM-25295-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	rcsb pdb:6I3M|rcsb pdb:6I7T|rcsb pdb:6QG0|rcsb pdb:6QG1|rcsb pdb:6QG2|rcsb pdb:6QG3|rcsb pdb:6QG5|rcsb pdb:6QG6|reactome:R-SCE-156827|reactome:R-SCE-381042|reactome:R-SCE-72649|reactome:R-SCE-72695|reactome:R-SCE-72702|reactome:R-SCE-72731|ensemblfungi:YER025W(gene)|ensemblfungi:YER025W_mRNA(transcript)|go:"GO:0000049"(tRNA binding)|go:"GO:0001731"(formation of translation preinitiation complex)|go:"GO:0003743"(translation initiation factor activity)|go:"GO:0003924"(GTPase activity)|go:"GO:0005525"(GTP binding)|go:"GO:0005829"(cytosol)|go:"GO:0005850"(eukaryotic translation initiation factor 2 complex)|go:"GO:0006413"(translational initiation)|go:"GO:0016282"(eukaryotic 43S preinitiation complex)|go:"GO:0031369"(translation initiation factor binding)|go:"GO:0033290"(eukaryotic 48S preinitiation complex)|go:"GO:0043614"(multi-eIF complex)|go:"GO:0045903"(positive regulation of translational fidelity)|interpro:IPR000795(Protein synthesis factor, GTP-binding)|interpro:IPR004161(Translation elongation factor EFTu/EF1A, domain 2)|interpro:IPR009000(Translation elongation and initiation factors/Ribosomal, beta-barrel)|interpro:IPR009001(Translation elongation factor EF1A/initiation factor IF2gamma, C-terminal)|interpro:IPR015256(Initiation factor eIF2 gamma, C terminal)|interpro:IPR027417|interpro:IPR044127|interpro:IPR044128|rcsb pdb:3J81|rcsb pdb:3JAP|rcsb pdb:3JAQ|rcsb pdb:4ZGN|rcsb pdb:4ZGQ|rcsb pdb:6FYX|rcsb pdb:6FYY|rcsb pdb:6GSM|rcsb pdb:6GSN|refseq:NP_010942.1|sgd:S000000827|mint:P32481|dip:DIP-2561N	interpro:IPR022967|interpro:IPR024054|interpro:IPR024055|interpro:IPR044126|rcsb pdb:1Q46|rcsb pdb:2A19|rcsb pdb:2A1A|rcsb pdb:3J81|rcsb pdb:3JAP|rcsb pdb:3JAQ|rcsb pdb:6FYX|rcsb pdb:6FYY|rcsb pdb:6GSM|rcsb pdb:6GSN|rcsb pdb:6I3M|rcsb pdb:6I7T|rcsb pdb:6JLY|rcsb pdb:6JLZ|rcsb pdb:6QG0|rcsb pdb:6QG1|rcsb pdb:6QG2|rcsb pdb:6QG3|rcsb pdb:6QG5|rcsb pdb:6QG6|reactome:R-SCE-156827|reactome:R-SCE-381042|reactome:R-SCE-72649|reactome:R-SCE-72695|reactome:R-SCE-72702|reactome:R-SCE-72731|ensemblfungi:YJR007W(gene)|ensemblfungi:YJR007W_mRNA(transcript)|go:"GO:0001731"(formation of translation preinitiation complex)|go:"GO:0003743"(translation initiation factor activity)|go:"GO:0005737"(cytoplasm)|go:"GO:0005829"(cytosol)|go:"GO:0005850"(eukaryotic translation initiation factor 2 complex)|go:"GO:0005851"(eukaryotic translation initiation factor 2B complex)|go:"GO:0010494"(cytoplasmic stress granule)|go:"GO:0033290"(eukaryotic 48S preinitiation complex)|go:"GO:0043022"(ribosome binding)|go:"GO:0043614"(multi-eIF complex)|interpro:IPR003029(S1, RNA binding)|interpro:IPR011488(Eukaryotic translation initiation factor 2, alpha subunit)|interpro:IPR012340(Nucleic acid-binding, OB-fold)|refseq:NP_012540.3|sgd:S000003767|mint:P20459|dip:DIP-2327N	-	crc64:D498AE62BC3E81CD	crc64:AF4F1C80303A4E98	figure legend:Fig. 1|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	-	2016/06/01	2016/06/01	rogid:KLaBewFsp1tygK0+hirsBIaMPEI559292	rogid:9/iGImP9/c8HSYwgmItME27z7lE559292	rigid:qTPyHktlj1ToFFmcNf8R0+DycuI	false	his tag:c-c	mutation with no effect:142-142|mutation with no effect:250-250	-	-	psi-mi:"MI:0818"(molecular weight estimation by coomasie staining)	psi-mi:"MI:0818"(molecular weight estimation by coomasie staining)
uniprotkb:P32481	uniprotkb:P09064	intact:EBI-8924|ensemblfungi:YER025W|uniprotkb:D3DLS4	intact:EBI-8920|ensemblfungi:YPL237W|uniprotkb:Q6B200|uniprotkb:D6W3D3	psi-mi:if2g_yeast(display_long)|uniprotkb:YER025W(locus name)|uniprotkb:GCD11(gene name)|psi-mi:GCD11(display_short)|uniprotkb:TIF213(gene name synonym)|uniprotkb:SUI4(gene name synonym)	psi-mi:if2b_yeast(display_long)|uniprotkb:YPL237W(locus name)|uniprotkb:SUI3(gene name)|psi-mi:SUI3(display_short)|uniprotkb:TIF212(gene name synonym)	psi-mi:"MI:0091"(chromatography technology)	Erickson et al. (1996)	pubmed:8947054|imex:IM-25295	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-11710491|imex:IM-25295-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	rcsb pdb:6I3M|rcsb pdb:6I7T|rcsb pdb:6QG0|rcsb pdb:6QG1|rcsb pdb:6QG2|rcsb pdb:6QG3|rcsb pdb:6QG5|rcsb pdb:6QG6|reactome:R-SCE-156827|reactome:R-SCE-381042|reactome:R-SCE-72649|reactome:R-SCE-72695|reactome:R-SCE-72702|reactome:R-SCE-72731|ensemblfungi:YER025W(gene)|ensemblfungi:YER025W_mRNA(transcript)|go:"GO:0000049"(tRNA binding)|go:"GO:0001731"(formation of translation preinitiation complex)|go:"GO:0003743"(translation initiation factor activity)|go:"GO:0003924"(GTPase activity)|go:"GO:0005525"(GTP binding)|go:"GO:0005829"(cytosol)|go:"GO:0005850"(eukaryotic translation initiation factor 2 complex)|go:"GO:0006413"(translational initiation)|go:"GO:0016282"(eukaryotic 43S preinitiation complex)|go:"GO:0031369"(translation initiation factor binding)|go:"GO:0033290"(eukaryotic 48S preinitiation complex)|go:"GO:0043614"(multi-eIF complex)|go:"GO:0045903"(positive regulation of translational fidelity)|interpro:IPR000795(Protein synthesis factor, GTP-binding)|interpro:IPR004161(Translation elongation factor EFTu/EF1A, domain 2)|interpro:IPR009000(Translation elongation and initiation factors/Ribosomal, beta-barrel)|interpro:IPR009001(Translation elongation factor EF1A/initiation factor IF2gamma, C-terminal)|interpro:IPR015256(Initiation factor eIF2 gamma, C terminal)|interpro:IPR027417|interpro:IPR044127|interpro:IPR044128|rcsb pdb:3J81|rcsb pdb:3JAP|rcsb pdb:3JAQ|rcsb pdb:4ZGN|rcsb pdb:4ZGQ|rcsb pdb:6FYX|rcsb pdb:6FYY|rcsb pdb:6GSM|rcsb pdb:6GSN|refseq:NP_010942.1|sgd:S000000827|mint:P32481|dip:DIP-2561N	ensemblfungi:YPL237W(gene)|ensemblfungi:YPL237W_mRNA(transcript)|go:"GO:0001731"(formation of translation preinitiation complex)|go:"GO:0001732"(formation of cytoplasmic translation initiation complex)|go:"GO:0003729"(mRNA binding)|go:"GO:0003743"(translation initiation factor activity)|go:"GO:0005829"(cytosol)|go:"GO:0005850"(eukaryotic translation initiation factor 2 complex)|go:"GO:0016282"(eukaryotic 43S preinitiation complex)|go:"GO:0031369"(translation initiation factor binding)|go:"GO:0043614"(multi-eIF complex)|go:"GO:0046872"(metal ion binding)|interpro:IPR002735(Translation initiation factor IF2/IF5)|interpro:IPR016189(Translation initiation factor IF2/IF5, N-terminal)|interpro:IPR016190(Translation initiation factor IF2/IF5, zinc-binding)|rcsb pdb:3J81|rcsb pdb:3JAP|rcsb pdb:3JAQ|rcsb pdb:6FYX|rcsb pdb:6FYY|rcsb pdb:6GSM|rcsb pdb:6GSN|rcsb pdb:6I3M|rcsb pdb:6I7T|rcsb pdb:6QG0|rcsb pdb:6QG1|rcsb pdb:6QG2|rcsb pdb:6QG3|rcsb pdb:6QG5|rcsb pdb:6QG6|reactome:R-SCE-72649|refseq:NP_015087.1|sgd:S000006158|reactome:R-SCE-156827|reactome:R-SCE-72695|reactome:R-SCE-72702|reactome:R-SCE-72731|dip:DIP-2310N|reactome:R-SCE-381042	-	crc64:D498AE62BC3E81CD	crc64:34DE9323876CEE95	figure legend:Fig. 1|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	-	2016/06/01	2016/06/01	rogid:KLaBewFsp1tygK0+hirsBIaMPEI559292	rogid:64fjjpMNrNM6gZW2BzacUQWRC1I559292	rigid:qTPyHktlj1ToFFmcNf8R0+DycuI	false	his tag:c-c	-	-	-	psi-mi:"MI:0818"(molecular weight estimation by coomasie staining)	psi-mi:"MI:0818"(molecular weight estimation by coomasie staining)
chebi:"CHEBI:17552"	intact:EBI-11710314	intact:EBI-1265684	-	psi-mi:gdp(display_short)|psi-mi:"CHEBI:17552"(display_long)	psi-mi:eif2_yeast(display_short)|psi-mi:EBI-11710314(display_long)|intact:IF-2 complex(complex synonym)|intact:IF2 complex(complex synonym)|intact:"GCD11:SUI2:SUI3"(complex systematic name)|intact:eIF2 complex(complex synonym)|intact:Eukaryotic translation initiation factor 2 complex(complex recommended name)|intact:eIF-2 complex(complex synonym)	psi-mi:"MI:0049"(filter binding)	Erickson et al. (1996)	pubmed:8947054|imex:IM-25295	taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)")	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0469"(IntAct)	intact:EBI-11711128|imex:IM-25295-2	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0328"(small molecule)	psi-mi:"MI:1302"(stable complex)	-	pubmed:8947054(see-also)|intact:EBI-11710491(exp-evidence)|go:"GO:0005525"(GTP binding)|go:"GO:0003743"(translation initiation factor activity)|go:"GO:1990856"(methionyl-initiator methionine tRNA binding)|go:"GO:0006413"(translational initiation)|go:"GO:0005840"(ribosome)|evidence ontology:"ECO:0000353"|go:"GO:0005850"(eukaryotic translation initiation factor 2 complex)|complex portal:CPX-427(complex-primary)	-	-	curated-complex:"Delivers initiator methionyl-tRNA to the 40S ribosomal subunit in a eukaryotic translation initiation factor 2 (eIF2).GTP.Met-tRNA(Met) ternary complex. The resulting 43S complex, which also includes eIF3 and eIF1A, binds at or near the 5-prime end of capped eukaryotic messenger RNAs. Recognition of the AUG codon translational start site is accompanied by GTP hydrolysis (stimulated by the eIF5 complex), which releases Met-tRNA to the ribosomal peptidyl site and converts eIF2-GTP to eIF2-GDP. Binding of nucleotide exchange factor eIF2B complex (CPX-429) replaces GDP again for GTP. This activity is inhibited when phosphorylated eIF2 (alpha subunit) binds to eIF2B.  Phosphorylation of eIF2 occurs in response to nutrient starvation and thus prevents further protein synthesis."|complex-assembly:Heterotrimer|complex-properties:Molecular weight = 125 kDa	figure legend:Fig.3A|comment:"A lys250arg mutation in the sequence of the gamma subunit of the complex (P32481) showed an approximately 30-fold increase in the koff value for GDP."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2016/06/01	2016/06/02	-	-	-	false	3h radiolabel:?-?	his tag:c-c	-	-	psi-mi:"MI:0866"(tag visualisation)	psi-mi:"MI:0866"(tag visualisation)
chebi:"CHEBI:15996"	intact:EBI-11710314	intact:EBI-989053|chebi:"CHEBI:42934"|chebi:"CHEBI:5234"|chebi:"CHEBI:13342"|chebi:"CHEBI:24451"	-	psi-mi:gtp(display_short)|psi-mi:"CHEBI:15996"(display_long)|intact:5'-GTP(synonym)|intact:GTP(synonym)|intact:Guanosine 5'-triphosphate(synonym)|intact:guanosine 5'-triphosphoric acid(synonym)|intact:guanosine triphosphate(synonym)|intact:GUANOSINE-5'-TRIPHOSPHATE(synonym)|intact:H4gtp(synonym)|intact:"guanosine 5'-(tetrahydrogen triphosphate)"(iupac name)	psi-mi:eif2_yeast(display_short)|psi-mi:EBI-11710314(display_long)|intact:IF-2 complex(complex synonym)|intact:IF2 complex(complex synonym)|intact:"GCD11:SUI2:SUI3"(complex systematic name)|intact:eIF2 complex(complex synonym)|intact:Eukaryotic translation initiation factor 2 complex(complex recommended name)|intact:eIF-2 complex(complex synonym)	psi-mi:"MI:0049"(filter binding)	Erickson et al. (1996)	pubmed:8947054|imex:IM-25295	taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)")	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0469"(IntAct)	intact:EBI-11711142|imex:IM-25295-4	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0328"(small molecule)	psi-mi:"MI:1302"(stable complex)	-	pubmed:8947054(see-also)|intact:EBI-11710491(exp-evidence)|go:"GO:0005525"(GTP binding)|go:"GO:0003743"(translation initiation factor activity)|go:"GO:1990856"(methionyl-initiator methionine tRNA binding)|go:"GO:0006413"(translational initiation)|go:"GO:0005840"(ribosome)|evidence ontology:"ECO:0000353"|go:"GO:0005850"(eukaryotic translation initiation factor 2 complex)|complex portal:CPX-427(complex-primary)	-	author-identifiers:"InChI=1/C10H16N5O14P3/c11-10-13-7-4(8(18)14-10)12-2-15(7)9-6(17)5(16)3(27-9)1-26-31(22,23)29-32(24,25)28-30(19,20)21/h2-3,5-6,9,16-17H,1H2,(H,22,23)(H,24,25)(H2,19,20,21)(H3,11,13,14,18)/t3-,5-,6-,9-/m1/s1/f/h14,19-20,22,24H,11H2"	curated-complex:"Delivers initiator methionyl-tRNA to the 40S ribosomal subunit in a eukaryotic translation initiation factor 2 (eIF2).GTP.Met-tRNA(Met) ternary complex. The resulting 43S complex, which also includes eIF3 and eIF1A, binds at or near the 5-prime end of capped eukaryotic messenger RNAs. Recognition of the AUG codon translational start site is accompanied by GTP hydrolysis (stimulated by the eIF5 complex), which releases Met-tRNA to the ribosomal peptidyl site and converts eIF2-GTP to eIF2-GDP. Binding of nucleotide exchange factor eIF2B complex (CPX-429) replaces GDP again for GTP. This activity is inhibited when phosphorylated eIF2 (alpha subunit) binds to eIF2B.  Phosphorylation of eIF2 occurs in response to nutrient starvation and thus prevents further protein synthesis."|complex-assembly:Heterotrimer|complex-properties:Molecular weight = 125 kDa	figure legend:Fig. 3B, 4A|comment:"Met-tRNAi(Met) appears to have a cooperative effect on GTP binding."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2016/06/01	2016/08/15	-	-	-	false	32p radiolabel:?-?	his tag:c-c	-	-	psi-mi:"MI:0866"(tag visualisation)	psi-mi:"MI:0866"(tag visualisation)
intact:EBI-11710314	intact:EBI-11703304	-	rnacentral:URS00005350CD_559292	psi-mi:eif2_yeast(display_short)|psi-mi:EBI-11710314(display_long)|intact:IF-2 complex(complex synonym)|intact:IF2 complex(complex synonym)|intact:"GCD11:SUI2:SUI3"(complex systematic name)|intact:eIF2 complex(complex synonym)|intact:Eukaryotic translation initiation factor 2 complex(complex recommended name)|intact:eIF-2 complex(complex synonym)	psi-mi:trna imet(display_short)|psi-mi:EBI-11703304(display_long)	psi-mi:"MI:0049"(filter binding)	Erickson et al. (1996)	pubmed:8947054|imex:IM-25295	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0469"(IntAct)	intact:EBI-11711150|imex:IM-25295-6	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:1302"(stable complex)	psi-mi:"MI:0325"(transfer rna)	pubmed:8947054(see-also)|intact:EBI-11710491(exp-evidence)|go:"GO:0005525"(GTP binding)|go:"GO:0003743"(translation initiation factor activity)|go:"GO:1990856"(methionyl-initiator methionine tRNA binding)|go:"GO:0006413"(translational initiation)|go:"GO:0005840"(ribosome)|evidence ontology:"ECO:0000353"|go:"GO:0005850"(eukaryotic translation initiation factor 2 complex)|complex portal:CPX-427(complex-primary)	-	-	curated-complex:"Delivers initiator methionyl-tRNA to the 40S ribosomal subunit in a eukaryotic translation initiation factor 2 (eIF2).GTP.Met-tRNA(Met) ternary complex. The resulting 43S complex, which also includes eIF3 and eIF1A, binds at or near the 5-prime end of capped eukaryotic messenger RNAs. Recognition of the AUG codon translational start site is accompanied by GTP hydrolysis (stimulated by the eIF5 complex), which releases Met-tRNA to the ribosomal peptidyl site and converts eIF2-GTP to eIF2-GDP. Binding of nucleotide exchange factor eIF2B complex (CPX-429) replaces GDP again for GTP. This activity is inhibited when phosphorylated eIF2 (alpha subunit) binds to eIF2B.  Phosphorylation of eIF2 occurs in response to nutrient starvation and thus prevents further protein synthesis."|complex-assembly:Heterotrimer|complex-properties:Molecular weight = 125 kDa	-	figure legend:Fig. 2A, 2B|comment:"A tyr142his mutation in the sequence of the gamma subunit of the complex (P32481) prevented Met-tRNAi(Met) binding"|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	kd:1.5x10^-8(molar)	2016/06/01	2016/08/15	-	-	-	false	binding-associated region:?-?|his tag:c-c	3h radiolabel:?-?	-	-	psi-mi:"MI:0866"(tag visualisation)	psi-mi:"MI:0866"(tag visualisation)
chebi:"CHEBI:15996"	intact:EBI-11710314	intact:EBI-989053|chebi:"CHEBI:42934"|chebi:"CHEBI:5234"|chebi:"CHEBI:13342"|chebi:"CHEBI:24451"	-	psi-mi:gtp(display_short)|psi-mi:"CHEBI:15996"(display_long)|intact:5'-GTP(synonym)|intact:GTP(synonym)|intact:Guanosine 5'-triphosphate(synonym)|intact:guanosine 5'-triphosphoric acid(synonym)|intact:guanosine triphosphate(synonym)|intact:GUANOSINE-5'-TRIPHOSPHATE(synonym)|intact:H4gtp(synonym)|intact:"guanosine 5'-(tetrahydrogen triphosphate)"(iupac name)	psi-mi:eif2_yeast(display_short)|psi-mi:EBI-11710314(display_long)|intact:IF-2 complex(complex synonym)|intact:IF2 complex(complex synonym)|intact:"GCD11:SUI2:SUI3"(complex systematic name)|intact:eIF2 complex(complex synonym)|intact:Eukaryotic translation initiation factor 2 complex(complex recommended name)|intact:eIF-2 complex(complex synonym)	psi-mi:"MI:0049"(filter binding)	Erickson et al. (1996)	pubmed:8947054|imex:IM-25295	taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)")	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0469"(IntAct)	intact:EBI-11711150|imex:IM-25295-6	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0684"(ancillary)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0328"(small molecule)	psi-mi:"MI:1302"(stable complex)	-	pubmed:8947054(see-also)|intact:EBI-11710491(exp-evidence)|go:"GO:0005525"(GTP binding)|go:"GO:0003743"(translation initiation factor activity)|go:"GO:1990856"(methionyl-initiator methionine tRNA binding)|go:"GO:0006413"(translational initiation)|go:"GO:0005840"(ribosome)|evidence ontology:"ECO:0000353"|go:"GO:0005850"(eukaryotic translation initiation factor 2 complex)|complex portal:CPX-427(complex-primary)	-	author-identifiers:"InChI=1/C10H16N5O14P3/c11-10-13-7-4(8(18)14-10)12-2-15(7)9-6(17)5(16)3(27-9)1-26-31(22,23)29-32(24,25)28-30(19,20)21/h2-3,5-6,9,16-17H,1H2,(H,22,23)(H,24,25)(H2,19,20,21)(H3,11,13,14,18)/t3-,5-,6-,9-/m1/s1/f/h14,19-20,22,24H,11H2"	curated-complex:"Delivers initiator methionyl-tRNA to the 40S ribosomal subunit in a eukaryotic translation initiation factor 2 (eIF2).GTP.Met-tRNA(Met) ternary complex. The resulting 43S complex, which also includes eIF3 and eIF1A, binds at or near the 5-prime end of capped eukaryotic messenger RNAs. Recognition of the AUG codon translational start site is accompanied by GTP hydrolysis (stimulated by the eIF5 complex), which releases Met-tRNA to the ribosomal peptidyl site and converts eIF2-GTP to eIF2-GDP. Binding of nucleotide exchange factor eIF2B complex (CPX-429) replaces GDP again for GTP. This activity is inhibited when phosphorylated eIF2 (alpha subunit) binds to eIF2B.  Phosphorylation of eIF2 occurs in response to nutrient starvation and thus prevents further protein synthesis."|complex-assembly:Heterotrimer|complex-properties:Molecular weight = 125 kDa	figure legend:Fig. 2A, 2B|comment:"A tyr142his mutation in the sequence of the gamma subunit of the complex (P32481) prevented Met-tRNAi(Met) binding"|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	kd:1.5x10^-8(molar)	2016/06/01	2016/08/15	-	-	-	false	binding-associated region:?-?	binding-associated region:?-?|his tag:c-c	-	-	psi-mi:"MI:0866"(tag visualisation)	psi-mi:"MI:0866"(tag visualisation)
intact:EBI-11710314	intact:EBI-11703304	-	rnacentral:URS00005350CD_559292	psi-mi:eif2_yeast(display_short)|psi-mi:EBI-11710314(display_long)|intact:IF-2 complex(complex synonym)|intact:IF2 complex(complex synonym)|intact:"GCD11:SUI2:SUI3"(complex systematic name)|intact:eIF2 complex(complex synonym)|intact:Eukaryotic translation initiation factor 2 complex(complex recommended name)|intact:eIF-2 complex(complex synonym)	psi-mi:trna imet(display_short)|psi-mi:EBI-11703304(display_long)	psi-mi:"MI:0049"(filter binding)	Erickson et al. (1996)	pubmed:8947054|imex:IM-25295	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0469"(IntAct)	intact:EBI-11874009|imex:IM-25295-8	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:1302"(stable complex)	psi-mi:"MI:0325"(transfer rna)	pubmed:8947054(see-also)|intact:EBI-11710491(exp-evidence)|go:"GO:0005525"(GTP binding)|go:"GO:0003743"(translation initiation factor activity)|go:"GO:1990856"(methionyl-initiator methionine tRNA binding)|go:"GO:0006413"(translational initiation)|go:"GO:0005840"(ribosome)|evidence ontology:"ECO:0000353"|go:"GO:0005850"(eukaryotic translation initiation factor 2 complex)|complex portal:CPX-427(complex-primary)	-	-	curated-complex:"Delivers initiator methionyl-tRNA to the 40S ribosomal subunit in a eukaryotic translation initiation factor 2 (eIF2).GTP.Met-tRNA(Met) ternary complex. The resulting 43S complex, which also includes eIF3 and eIF1A, binds at or near the 5-prime end of capped eukaryotic messenger RNAs. Recognition of the AUG codon translational start site is accompanied by GTP hydrolysis (stimulated by the eIF5 complex), which releases Met-tRNA to the ribosomal peptidyl site and converts eIF2-GTP to eIF2-GDP. Binding of nucleotide exchange factor eIF2B complex (CPX-429) replaces GDP again for GTP. This activity is inhibited when phosphorylated eIF2 (alpha subunit) binds to eIF2B.  Phosphorylation of eIF2 occurs in response to nutrient starvation and thus prevents further protein synthesis."|complex-assembly:Heterotrimer|complex-properties:Molecular weight = 125 kDa	-	figure legend:Fig. 4A, 4B|comment:"A tyr142his mutation in the sequence of the gamma subunit of the complex (P32481) prevented Met-tRNAi(Met) binding"|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	kd:1.5x10^-8(molar)	2016/06/01	2016/08/15	-	-	-	false	binding-associated region:?-?|his tag:c-c	3h radiolabel:?-?	-	-	psi-mi:"MI:0866"(tag visualisation)	psi-mi:"MI:0866"(tag visualisation)
chebi:"CHEBI:15996"	intact:EBI-11710314	intact:EBI-989053|chebi:"CHEBI:42934"|chebi:"CHEBI:5234"|chebi:"CHEBI:13342"|chebi:"CHEBI:24451"	-	psi-mi:gtp(display_short)|psi-mi:"CHEBI:15996"(display_long)|intact:5'-GTP(synonym)|intact:GTP(synonym)|intact:Guanosine 5'-triphosphate(synonym)|intact:guanosine 5'-triphosphoric acid(synonym)|intact:guanosine triphosphate(synonym)|intact:GUANOSINE-5'-TRIPHOSPHATE(synonym)|intact:H4gtp(synonym)|intact:"guanosine 5'-(tetrahydrogen triphosphate)"(iupac name)	psi-mi:eif2_yeast(display_short)|psi-mi:EBI-11710314(display_long)|intact:IF-2 complex(complex synonym)|intact:IF2 complex(complex synonym)|intact:"GCD11:SUI2:SUI3"(complex systematic name)|intact:eIF2 complex(complex synonym)|intact:Eukaryotic translation initiation factor 2 complex(complex recommended name)|intact:eIF-2 complex(complex synonym)	psi-mi:"MI:0049"(filter binding)	Erickson et al. (1996)	pubmed:8947054|imex:IM-25295	taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)")	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0469"(IntAct)	intact:EBI-11874009|imex:IM-25295-8	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0684"(ancillary)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0328"(small molecule)	psi-mi:"MI:1302"(stable complex)	-	pubmed:8947054(see-also)|intact:EBI-11710491(exp-evidence)|go:"GO:0005525"(GTP binding)|go:"GO:0003743"(translation initiation factor activity)|go:"GO:1990856"(methionyl-initiator methionine tRNA binding)|go:"GO:0006413"(translational initiation)|go:"GO:0005840"(ribosome)|evidence ontology:"ECO:0000353"|go:"GO:0005850"(eukaryotic translation initiation factor 2 complex)|complex portal:CPX-427(complex-primary)	-	author-identifiers:"InChI=1/C10H16N5O14P3/c11-10-13-7-4(8(18)14-10)12-2-15(7)9-6(17)5(16)3(27-9)1-26-31(22,23)29-32(24,25)28-30(19,20)21/h2-3,5-6,9,16-17H,1H2,(H,22,23)(H,24,25)(H2,19,20,21)(H3,11,13,14,18)/t3-,5-,6-,9-/m1/s1/f/h14,19-20,22,24H,11H2"	curated-complex:"Delivers initiator methionyl-tRNA to the 40S ribosomal subunit in a eukaryotic translation initiation factor 2 (eIF2).GTP.Met-tRNA(Met) ternary complex. The resulting 43S complex, which also includes eIF3 and eIF1A, binds at or near the 5-prime end of capped eukaryotic messenger RNAs. Recognition of the AUG codon translational start site is accompanied by GTP hydrolysis (stimulated by the eIF5 complex), which releases Met-tRNA to the ribosomal peptidyl site and converts eIF2-GTP to eIF2-GDP. Binding of nucleotide exchange factor eIF2B complex (CPX-429) replaces GDP again for GTP. This activity is inhibited when phosphorylated eIF2 (alpha subunit) binds to eIF2B.  Phosphorylation of eIF2 occurs in response to nutrient starvation and thus prevents further protein synthesis."|complex-assembly:Heterotrimer|complex-properties:Molecular weight = 125 kDa	figure legend:Fig. 4A, 4B|comment:"A tyr142his mutation in the sequence of the gamma subunit of the complex (P32481) prevented Met-tRNAi(Met) binding"|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	kd:1.5x10^-8(molar)	2016/06/01	2016/08/15	-	-	-	false	binding-associated region:?-?|32p radiolabel:?-?	binding-associated region:?-?|his tag:c-c	-	-	psi-mi:"MI:0866"(tag visualisation)	psi-mi:"MI:0866"(tag visualisation)
chebi:"CHEBI:17552"	intact:EBI-11710314	intact:EBI-1265684	-	psi-mi:gdp(display_short)|psi-mi:"CHEBI:17552"(display_long)	psi-mi:eif2_yeast(display_short)|psi-mi:EBI-11710314(display_long)|intact:IF-2 complex(complex synonym)|intact:IF2 complex(complex synonym)|intact:"GCD11:SUI2:SUI3"(complex systematic name)|intact:eIF2 complex(complex synonym)|intact:Eukaryotic translation initiation factor 2 complex(complex recommended name)|intact:eIF-2 complex(complex synonym)	psi-mi:"MI:0049"(filter binding)	Erickson et al. (1996)	pubmed:8947054|imex:IM-25295	taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)")	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0469"(IntAct)	intact:EBI-11874371|imex:IM-25295-10	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0941"(competitor)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0328"(small molecule)	psi-mi:"MI:1302"(stable complex)	-	pubmed:8947054(see-also)|intact:EBI-11710491(exp-evidence)|go:"GO:0005525"(GTP binding)|go:"GO:0003743"(translation initiation factor activity)|go:"GO:1990856"(methionyl-initiator methionine tRNA binding)|go:"GO:0006413"(translational initiation)|go:"GO:0005840"(ribosome)|evidence ontology:"ECO:0000353"|go:"GO:0005850"(eukaryotic translation initiation factor 2 complex)|complex portal:CPX-427(complex-primary)	-	-	curated-complex:"Delivers initiator methionyl-tRNA to the 40S ribosomal subunit in a eukaryotic translation initiation factor 2 (eIF2).GTP.Met-tRNA(Met) ternary complex. The resulting 43S complex, which also includes eIF3 and eIF1A, binds at or near the 5-prime end of capped eukaryotic messenger RNAs. Recognition of the AUG codon translational start site is accompanied by GTP hydrolysis (stimulated by the eIF5 complex), which releases Met-tRNA to the ribosomal peptidyl site and converts eIF2-GTP to eIF2-GDP. Binding of nucleotide exchange factor eIF2B complex (CPX-429) replaces GDP again for GTP. This activity is inhibited when phosphorylated eIF2 (alpha subunit) binds to eIF2B.  Phosphorylation of eIF2 occurs in response to nutrient starvation and thus prevents further protein synthesis."|complex-assembly:Heterotrimer|complex-properties:Molecular weight = 125 kDa	figure legend:Fig.3A|comment:"A lys250arg mutation in the sequence of the gamma subunit of the complex (P32481) showed an approximately 30-fold increase in the koff value for GDP."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2016/06/01	2016/07/13	-	-	-	false	-	his tag:c-c	-	-	psi-mi:"MI:0866"(tag visualisation)	psi-mi:"MI:0866"(tag visualisation)
chebi:"CHEBI:17552"	intact:EBI-11710314	intact:EBI-1265684	-	psi-mi:gdp(display_short)|psi-mi:"CHEBI:17552"(display_long)	psi-mi:eif2_yeast(display_short)|psi-mi:EBI-11710314(display_long)|intact:IF-2 complex(complex synonym)|intact:IF2 complex(complex synonym)|intact:"GCD11:SUI2:SUI3"(complex systematic name)|intact:eIF2 complex(complex synonym)|intact:Eukaryotic translation initiation factor 2 complex(complex recommended name)|intact:eIF-2 complex(complex synonym)	psi-mi:"MI:0049"(filter binding)	Erickson et al. (1996)	pubmed:8947054|imex:IM-25295	taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)")	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0469"(IntAct)	intact:EBI-11874371|imex:IM-25295-10	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0328"(small molecule)	psi-mi:"MI:1302"(stable complex)	-	pubmed:8947054(see-also)|intact:EBI-11710491(exp-evidence)|go:"GO:0005525"(GTP binding)|go:"GO:0003743"(translation initiation factor activity)|go:"GO:1990856"(methionyl-initiator methionine tRNA binding)|go:"GO:0006413"(translational initiation)|go:"GO:0005840"(ribosome)|evidence ontology:"ECO:0000353"|go:"GO:0005850"(eukaryotic translation initiation factor 2 complex)|complex portal:CPX-427(complex-primary)	-	-	curated-complex:"Delivers initiator methionyl-tRNA to the 40S ribosomal subunit in a eukaryotic translation initiation factor 2 (eIF2).GTP.Met-tRNA(Met) ternary complex. The resulting 43S complex, which also includes eIF3 and eIF1A, binds at or near the 5-prime end of capped eukaryotic messenger RNAs. Recognition of the AUG codon translational start site is accompanied by GTP hydrolysis (stimulated by the eIF5 complex), which releases Met-tRNA to the ribosomal peptidyl site and converts eIF2-GTP to eIF2-GDP. Binding of nucleotide exchange factor eIF2B complex (CPX-429) replaces GDP again for GTP. This activity is inhibited when phosphorylated eIF2 (alpha subunit) binds to eIF2B.  Phosphorylation of eIF2 occurs in response to nutrient starvation and thus prevents further protein synthesis."|complex-assembly:Heterotrimer|complex-properties:Molecular weight = 125 kDa	figure legend:Fig.3A|comment:"A lys250arg mutation in the sequence of the gamma subunit of the complex (P32481) showed an approximately 30-fold increase in the koff value for GDP."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2016/06/01	2016/07/13	-	-	-	false	3h radiolabel:?-?	his tag:c-c	-	-	psi-mi:"MI:0866"(tag visualisation)	psi-mi:"MI:0866"(tag visualisation)
chebi:"CHEBI:15996"	intact:EBI-11710314	intact:EBI-989053|chebi:"CHEBI:42934"|chebi:"CHEBI:5234"|chebi:"CHEBI:13342"|chebi:"CHEBI:24451"	-	psi-mi:gtp(display_short)|psi-mi:"CHEBI:15996"(display_long)|intact:5'-GTP(synonym)|intact:GTP(synonym)|intact:Guanosine 5'-triphosphate(synonym)|intact:guanosine 5'-triphosphoric acid(synonym)|intact:guanosine triphosphate(synonym)|intact:GUANOSINE-5'-TRIPHOSPHATE(synonym)|intact:H4gtp(synonym)|intact:"guanosine 5'-(tetrahydrogen triphosphate)"(iupac name)	psi-mi:eif2_yeast(display_short)|psi-mi:EBI-11710314(display_long)|intact:IF-2 complex(complex synonym)|intact:IF2 complex(complex synonym)|intact:"GCD11:SUI2:SUI3"(complex systematic name)|intact:eIF2 complex(complex synonym)|intact:Eukaryotic translation initiation factor 2 complex(complex recommended name)|intact:eIF-2 complex(complex synonym)	psi-mi:"MI:0049"(filter binding)	Erickson et al. (1996)	pubmed:8947054|imex:IM-25295	taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)")	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0469"(IntAct)	intact:EBI-11874434|imex:IM-25295-12	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0328"(small molecule)	psi-mi:"MI:1302"(stable complex)	-	pubmed:8947054(see-also)|intact:EBI-11710491(exp-evidence)|go:"GO:0005525"(GTP binding)|go:"GO:0003743"(translation initiation factor activity)|go:"GO:1990856"(methionyl-initiator methionine tRNA binding)|go:"GO:0006413"(translational initiation)|go:"GO:0005840"(ribosome)|evidence ontology:"ECO:0000353"|go:"GO:0005850"(eukaryotic translation initiation factor 2 complex)|complex portal:CPX-427(complex-primary)	-	author-identifiers:"InChI=1/C10H16N5O14P3/c11-10-13-7-4(8(18)14-10)12-2-15(7)9-6(17)5(16)3(27-9)1-26-31(22,23)29-32(24,25)28-30(19,20)21/h2-3,5-6,9,16-17H,1H2,(H,22,23)(H,24,25)(H2,19,20,21)(H3,11,13,14,18)/t3-,5-,6-,9-/m1/s1/f/h14,19-20,22,24H,11H2"	curated-complex:"Delivers initiator methionyl-tRNA to the 40S ribosomal subunit in a eukaryotic translation initiation factor 2 (eIF2).GTP.Met-tRNA(Met) ternary complex. The resulting 43S complex, which also includes eIF3 and eIF1A, binds at or near the 5-prime end of capped eukaryotic messenger RNAs. Recognition of the AUG codon translational start site is accompanied by GTP hydrolysis (stimulated by the eIF5 complex), which releases Met-tRNA to the ribosomal peptidyl site and converts eIF2-GTP to eIF2-GDP. Binding of nucleotide exchange factor eIF2B complex (CPX-429) replaces GDP again for GTP. This activity is inhibited when phosphorylated eIF2 (alpha subunit) binds to eIF2B.  Phosphorylation of eIF2 occurs in response to nutrient starvation and thus prevents further protein synthesis."|complex-assembly:Heterotrimer|complex-properties:Molecular weight = 125 kDa	figure legend:Fig. 3B, 4A|comment:"Met-tRNAi(Met) appears to have a cooperative effect on GTP binding."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2016/06/01	2016/08/15	-	-	-	false	32p radiolabel:?-?	his tag:c-c	-	-	psi-mi:"MI:0866"(tag visualisation)	psi-mi:"MI:0866"(tag visualisation)
chebi:"CHEBI:17552"	intact:EBI-11710314	intact:EBI-1265684	-	psi-mi:gdp(display_short)|psi-mi:"CHEBI:17552"(display_long)	psi-mi:eif2_yeast(display_short)|psi-mi:EBI-11710314(display_long)|intact:IF-2 complex(complex synonym)|intact:IF2 complex(complex synonym)|intact:"GCD11:SUI2:SUI3"(complex systematic name)|intact:eIF2 complex(complex synonym)|intact:Eukaryotic translation initiation factor 2 complex(complex recommended name)|intact:eIF-2 complex(complex synonym)	psi-mi:"MI:0049"(filter binding)	Erickson et al. (1996)	pubmed:8947054|imex:IM-25295	taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)")	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0469"(IntAct)	intact:EBI-11874434|imex:IM-25295-12	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0941"(competitor)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0328"(small molecule)	psi-mi:"MI:1302"(stable complex)	-	pubmed:8947054(see-also)|intact:EBI-11710491(exp-evidence)|go:"GO:0005525"(GTP binding)|go:"GO:0003743"(translation initiation factor activity)|go:"GO:1990856"(methionyl-initiator methionine tRNA binding)|go:"GO:0006413"(translational initiation)|go:"GO:0005840"(ribosome)|evidence ontology:"ECO:0000353"|go:"GO:0005850"(eukaryotic translation initiation factor 2 complex)|complex portal:CPX-427(complex-primary)	-	-	curated-complex:"Delivers initiator methionyl-tRNA to the 40S ribosomal subunit in a eukaryotic translation initiation factor 2 (eIF2).GTP.Met-tRNA(Met) ternary complex. The resulting 43S complex, which also includes eIF3 and eIF1A, binds at or near the 5-prime end of capped eukaryotic messenger RNAs. Recognition of the AUG codon translational start site is accompanied by GTP hydrolysis (stimulated by the eIF5 complex), which releases Met-tRNA to the ribosomal peptidyl site and converts eIF2-GTP to eIF2-GDP. Binding of nucleotide exchange factor eIF2B complex (CPX-429) replaces GDP again for GTP. This activity is inhibited when phosphorylated eIF2 (alpha subunit) binds to eIF2B.  Phosphorylation of eIF2 occurs in response to nutrient starvation and thus prevents further protein synthesis."|complex-assembly:Heterotrimer|complex-properties:Molecular weight = 125 kDa	figure legend:Fig. 3B, 4A|comment:"Met-tRNAi(Met) appears to have a cooperative effect on GTP binding."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2016/06/01	2016/08/15	-	-	-	false	-	his tag:c-c	-	-	psi-mi:"MI:0866"(tag visualisation)	psi-mi:"MI:0866"(tag visualisation)
chebi:"CHEBI:17552"	intact:EBI-11710314	intact:EBI-1265684	-	psi-mi:gdp(display_short)|psi-mi:"CHEBI:17552"(display_long)	psi-mi:eif2_yeast(display_short)|psi-mi:EBI-11710314(display_long)|intact:IF-2 complex(complex synonym)|intact:IF2 complex(complex synonym)|intact:"GCD11:SUI2:SUI3"(complex systematic name)|intact:eIF2 complex(complex synonym)|intact:Eukaryotic translation initiation factor 2 complex(complex recommended name)|intact:eIF-2 complex(complex synonym)	psi-mi:"MI:0049"(filter binding)	Erickson et al. (1996)	pubmed:8947054|imex:IM-25295	taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)")	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0469"(IntAct)	intact:EBI-11874469|imex:IM-25295-14	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0941"(competitor)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0328"(small molecule)	psi-mi:"MI:1302"(stable complex)	-	pubmed:8947054(see-also)|intact:EBI-11710491(exp-evidence)|go:"GO:0005525"(GTP binding)|go:"GO:0003743"(translation initiation factor activity)|go:"GO:1990856"(methionyl-initiator methionine tRNA binding)|go:"GO:0006413"(translational initiation)|go:"GO:0005840"(ribosome)|evidence ontology:"ECO:0000353"|go:"GO:0005850"(eukaryotic translation initiation factor 2 complex)|complex portal:CPX-427(complex-primary)	-	-	curated-complex:"Delivers initiator methionyl-tRNA to the 40S ribosomal subunit in a eukaryotic translation initiation factor 2 (eIF2).GTP.Met-tRNA(Met) ternary complex. The resulting 43S complex, which also includes eIF3 and eIF1A, binds at or near the 5-prime end of capped eukaryotic messenger RNAs. Recognition of the AUG codon translational start site is accompanied by GTP hydrolysis (stimulated by the eIF5 complex), which releases Met-tRNA to the ribosomal peptidyl site and converts eIF2-GTP to eIF2-GDP. Binding of nucleotide exchange factor eIF2B complex (CPX-429) replaces GDP again for GTP. This activity is inhibited when phosphorylated eIF2 (alpha subunit) binds to eIF2B.  Phosphorylation of eIF2 occurs in response to nutrient starvation and thus prevents further protein synthesis."|complex-assembly:Heterotrimer|complex-properties:Molecular weight = 125 kDa	figure legend:Fig. 4A, 4B|comment:"A tyr142his mutation in the sequence of the gamma subunit of the complex (P32481) prevented Met-tRNAi(Met) binding"|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	kd:1.5x10^-8(molar)	2016/06/01	2016/08/15	-	-	-	false	-	binding-associated region:?-?|his tag:c-c	-	-	psi-mi:"MI:0866"(tag visualisation)	psi-mi:"MI:0866"(tag visualisation)
intact:EBI-11710314	intact:EBI-11703304	-	rnacentral:URS00005350CD_559292	psi-mi:eif2_yeast(display_short)|psi-mi:EBI-11710314(display_long)|intact:IF-2 complex(complex synonym)|intact:IF2 complex(complex synonym)|intact:"GCD11:SUI2:SUI3"(complex systematic name)|intact:eIF2 complex(complex synonym)|intact:Eukaryotic translation initiation factor 2 complex(complex recommended name)|intact:eIF-2 complex(complex synonym)	psi-mi:trna imet(display_short)|psi-mi:EBI-11703304(display_long)	psi-mi:"MI:0049"(filter binding)	Erickson et al. (1996)	pubmed:8947054|imex:IM-25295	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0469"(IntAct)	intact:EBI-11874469|imex:IM-25295-14	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0941"(competitor)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:1302"(stable complex)	psi-mi:"MI:0325"(transfer rna)	pubmed:8947054(see-also)|intact:EBI-11710491(exp-evidence)|go:"GO:0005525"(GTP binding)|go:"GO:0003743"(translation initiation factor activity)|go:"GO:1990856"(methionyl-initiator methionine tRNA binding)|go:"GO:0006413"(translational initiation)|go:"GO:0005840"(ribosome)|evidence ontology:"ECO:0000353"|go:"GO:0005850"(eukaryotic translation initiation factor 2 complex)|complex portal:CPX-427(complex-primary)	-	-	curated-complex:"Delivers initiator methionyl-tRNA to the 40S ribosomal subunit in a eukaryotic translation initiation factor 2 (eIF2).GTP.Met-tRNA(Met) ternary complex. The resulting 43S complex, which also includes eIF3 and eIF1A, binds at or near the 5-prime end of capped eukaryotic messenger RNAs. Recognition of the AUG codon translational start site is accompanied by GTP hydrolysis (stimulated by the eIF5 complex), which releases Met-tRNA to the ribosomal peptidyl site and converts eIF2-GTP to eIF2-GDP. Binding of nucleotide exchange factor eIF2B complex (CPX-429) replaces GDP again for GTP. This activity is inhibited when phosphorylated eIF2 (alpha subunit) binds to eIF2B.  Phosphorylation of eIF2 occurs in response to nutrient starvation and thus prevents further protein synthesis."|complex-assembly:Heterotrimer|complex-properties:Molecular weight = 125 kDa	-	figure legend:Fig. 4A, 4B|comment:"A tyr142his mutation in the sequence of the gamma subunit of the complex (P32481) prevented Met-tRNAi(Met) binding"|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	kd:1.5x10^-8(molar)	2016/06/01	2016/08/15	-	-	-	false	binding-associated region:?-?|his tag:c-c	3h radiolabel:?-?	-	-	psi-mi:"MI:0866"(tag visualisation)	psi-mi:"MI:0866"(tag visualisation)
chebi:"CHEBI:15996"	intact:EBI-11710314	intact:EBI-989053|chebi:"CHEBI:42934"|chebi:"CHEBI:5234"|chebi:"CHEBI:13342"|chebi:"CHEBI:24451"	-	psi-mi:gtp(display_short)|psi-mi:"CHEBI:15996"(display_long)|intact:5'-GTP(synonym)|intact:GTP(synonym)|intact:Guanosine 5'-triphosphate(synonym)|intact:guanosine 5'-triphosphoric acid(synonym)|intact:guanosine triphosphate(synonym)|intact:GUANOSINE-5'-TRIPHOSPHATE(synonym)|intact:H4gtp(synonym)|intact:"guanosine 5'-(tetrahydrogen triphosphate)"(iupac name)	psi-mi:eif2_yeast(display_short)|psi-mi:EBI-11710314(display_long)|intact:IF-2 complex(complex synonym)|intact:IF2 complex(complex synonym)|intact:"GCD11:SUI2:SUI3"(complex systematic name)|intact:eIF2 complex(complex synonym)|intact:Eukaryotic translation initiation factor 2 complex(complex recommended name)|intact:eIF-2 complex(complex synonym)	psi-mi:"MI:0049"(filter binding)	Erickson et al. (1996)	pubmed:8947054|imex:IM-25295	taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)")	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0469"(IntAct)	intact:EBI-11874469|imex:IM-25295-14	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0684"(ancillary)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0328"(small molecule)	psi-mi:"MI:1302"(stable complex)	-	pubmed:8947054(see-also)|intact:EBI-11710491(exp-evidence)|go:"GO:0005525"(GTP binding)|go:"GO:0003743"(translation initiation factor activity)|go:"GO:1990856"(methionyl-initiator methionine tRNA binding)|go:"GO:0006413"(translational initiation)|go:"GO:0005840"(ribosome)|evidence ontology:"ECO:0000353"|go:"GO:0005850"(eukaryotic translation initiation factor 2 complex)|complex portal:CPX-427(complex-primary)	-	author-identifiers:"InChI=1/C10H16N5O14P3/c11-10-13-7-4(8(18)14-10)12-2-15(7)9-6(17)5(16)3(27-9)1-26-31(22,23)29-32(24,25)28-30(19,20)21/h2-3,5-6,9,16-17H,1H2,(H,22,23)(H,24,25)(H2,19,20,21)(H3,11,13,14,18)/t3-,5-,6-,9-/m1/s1/f/h14,19-20,22,24H,11H2"	curated-complex:"Delivers initiator methionyl-tRNA to the 40S ribosomal subunit in a eukaryotic translation initiation factor 2 (eIF2).GTP.Met-tRNA(Met) ternary complex. The resulting 43S complex, which also includes eIF3 and eIF1A, binds at or near the 5-prime end of capped eukaryotic messenger RNAs. Recognition of the AUG codon translational start site is accompanied by GTP hydrolysis (stimulated by the eIF5 complex), which releases Met-tRNA to the ribosomal peptidyl site and converts eIF2-GTP to eIF2-GDP. Binding of nucleotide exchange factor eIF2B complex (CPX-429) replaces GDP again for GTP. This activity is inhibited when phosphorylated eIF2 (alpha subunit) binds to eIF2B.  Phosphorylation of eIF2 occurs in response to nutrient starvation and thus prevents further protein synthesis."|complex-assembly:Heterotrimer|complex-properties:Molecular weight = 125 kDa	figure legend:Fig. 4A, 4B|comment:"A tyr142his mutation in the sequence of the gamma subunit of the complex (P32481) prevented Met-tRNAi(Met) binding"|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	kd:1.5x10^-8(molar)	2016/06/01	2016/08/15	-	-	-	false	binding-associated region:?-?|32p radiolabel:?-?	binding-associated region:?-?|his tag:c-c	-	-	psi-mi:"MI:0866"(tag visualisation)	psi-mi:"MI:0866"(tag visualisation)