#ID(s) interactor A	ID(s) interactor B	Alt. ID(s) interactor A	Alt. ID(s) interactor B	Alias(es) interactor A	Alias(es) interactor B	Interaction detection method(s)	Publication 1st author(s)	Publication Identifier(s)	Taxid interactor A	Taxid interactor B	Interaction type(s)	Source database(s)	Interaction identifier(s)	Confidence value(s)	Expansion method(s)	Biological role(s) interactor A	Biological role(s) interactor B	Experimental role(s) interactor A	Experimental role(s) interactor B	Type(s) interactor A	Type(s) interactor B	Xref(s) interactor A	Xref(s) interactor B	Interaction Xref(s)	Annotation(s) interactor A	Annotation(s) interactor B	Interaction annotation(s)	Host organism(s)	Interaction parameter(s)	Creation date	Update date	Checksum(s) interactor A	Checksum(s) interactor B	Interaction Checksum(s)	Negative	Feature(s) interactor A	Feature(s) interactor B	Stoichiometry(s) interactor A	Stoichiometry(s) interactor B	Identification method participant A	Identification method participant B
uniprotkb:P04002	uniprotkb:P04002	intact:EBI-11769728|uniprotkb:Q7SIC4	intact:EBI-11769728|uniprotkb:Q7SIC4	psi-mi:anpa_pseam(display_long)|uniprotkb:Antifreeze protein A(gene name synonym)|uniprotkb:HPLC6(gene name synonym)	psi-mi:anpa_pseam(display_long)|uniprotkb:Antifreeze protein A(gene name synonym)|uniprotkb:HPLC6(gene name synonym)	psi-mi:"MI:0017"(classical fluorescence spectroscopy)	Dubé et al. (2016)	pubmed:27086686|imex:IM-25316	taxid:8265(Winter flounder)|taxid:8265(Pseudopleuronectes americanus)	taxid:8265(Winter flounder)|taxid:8265(Pseudopleuronectes americanus)	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0471"(MINT)	intact:EBI-11769741|imex:IM-25316-1	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	rcsb pdb:1ATF|rcsb pdb:1J5B|rcsb pdb:1WFA|rcsb pdb:1WFB|interpro:IPR000104(Antifreeze protein, type I)|go:"GO:0005615"(extracellular space)|go:"GO:0042802"(identical protein binding)|go:"GO:0050825"(ice binding)	rcsb pdb:1ATF|rcsb pdb:1J5B|rcsb pdb:1WFA|rcsb pdb:1WFB|interpro:IPR000104(Antifreeze protein, type I)|go:"GO:0005615"(extracellular space)|go:"GO:0042802"(identical protein binding)|go:"GO:0050825"(ice binding)	-	-	-	figure legend:f3 f6 f7 f8 f9|comment:"Equilibrium freezing of 15 mm wflAFP-6 in the presence of an ice seed resulted in strong ThT fluorescence (Fig. 3), while spontaneously nucleated rapid phase change in the absence of an ice seed and maintenance of a low temperature without freezing each produced far weaker signals similar to those of control samples held at 4 °C. Thus, it is the specific binding of wflAFP-6 to the ice surface that favours its transition to an amyloid structure, rather than temperature or phase changes that occur during the freezing process."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2016/06/14	2016/06/17	rogid:bWGG0F3GX4rZ3jlu2LxM1Q4TcXg8265	rogid:bWGG0F3GX4rZ3jlu2LxM1Q4TcXg8265	rigid:i+i/mj7QHm40+HdzFkhaVc30NKc	false	-	-	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
uniprotkb:P04002	uniprotkb:P04002	intact:EBI-11769728|uniprotkb:Q7SIC4	intact:EBI-11769728|uniprotkb:Q7SIC4	psi-mi:anpa_pseam(display_long)|uniprotkb:Antifreeze protein A(gene name synonym)|uniprotkb:HPLC6(gene name synonym)	psi-mi:anpa_pseam(display_long)|uniprotkb:Antifreeze protein A(gene name synonym)|uniprotkb:HPLC6(gene name synonym)	psi-mi:"MI:0040"(electron microscopy)	Dubé et al. (2016)	pubmed:27086686|imex:IM-25316	taxid:8265(Winter flounder)|taxid:8265(Pseudopleuronectes americanus)	taxid:8265(Winter flounder)|taxid:8265(Pseudopleuronectes americanus)	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0471"(MINT)	intact:EBI-11769759|imex:IM-25316-3	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	rcsb pdb:1ATF|rcsb pdb:1J5B|rcsb pdb:1WFA|rcsb pdb:1WFB|interpro:IPR000104(Antifreeze protein, type I)|go:"GO:0005615"(extracellular space)|go:"GO:0042802"(identical protein binding)|go:"GO:0050825"(ice binding)	rcsb pdb:1ATF|rcsb pdb:1J5B|rcsb pdb:1WFA|rcsb pdb:1WFB|interpro:IPR000104(Antifreeze protein, type I)|go:"GO:0005615"(extracellular space)|go:"GO:0042802"(identical protein binding)|go:"GO:0050825"(ice binding)	-	-	-	figure legend:f4|comment:"When examined by TEM, the samples that had undergone ice interaction (ice-seeded freezing) revealed fibrils (Fig. 4), whereas none were present in the control samples that were held unfrozen at 4 °C."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2016/06/14	2016/06/17	rogid:bWGG0F3GX4rZ3jlu2LxM1Q4TcXg8265	rogid:bWGG0F3GX4rZ3jlu2LxM1Q4TcXg8265	rigid:i+i/mj7QHm40+HdzFkhaVc30NKc	false	-	-	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
uniprotkb:P04002	uniprotkb:P04002	intact:EBI-11769728|uniprotkb:Q7SIC4	intact:EBI-11769728|uniprotkb:Q7SIC4	psi-mi:anpa_pseam(display_long)|uniprotkb:Antifreeze protein A(gene name synonym)|uniprotkb:HPLC6(gene name synonym)	psi-mi:anpa_pseam(display_long)|uniprotkb:Antifreeze protein A(gene name synonym)|uniprotkb:HPLC6(gene name synonym)	psi-mi:"MI:0071"(molecular sieving)	Dubé et al. (2016)	pubmed:27086686|imex:IM-25316	taxid:8265(Winter flounder)|taxid:8265(Pseudopleuronectes americanus)	taxid:8265(Winter flounder)|taxid:8265(Pseudopleuronectes americanus)	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0471"(MINT)	intact:EBI-11769765|imex:IM-25316-5	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	rcsb pdb:1ATF|rcsb pdb:1J5B|rcsb pdb:1WFA|rcsb pdb:1WFB|interpro:IPR000104(Antifreeze protein, type I)|go:"GO:0005615"(extracellular space)|go:"GO:0042802"(identical protein binding)|go:"GO:0050825"(ice binding)	rcsb pdb:1ATF|rcsb pdb:1J5B|rcsb pdb:1WFA|rcsb pdb:1WFB|interpro:IPR000104(Antifreeze protein, type I)|go:"GO:0005615"(extracellular space)|go:"GO:0042802"(identical protein binding)|go:"GO:0050825"(ice binding)	-	-	-	figure legend:f5|comment:"The protein size profile analysed by size exclusion chromatography also shifted in ice interaction samples compared with unfrozen (4 °C) controls (Fig. 5). Early-eluting protein with relative masses > 67 kDa (the largest marker) increased sharply (peaks 1 and 2), whereas the major peak (4) corresponding to a mass of 10 kDa, which is the predicted mass of monomeric wflAFP-6 in size exclusion chromatography due to its extended structure [21, 22], was diminished. These results suggest extensive protein association. A 32-kDa peak (3) was also present in both samples, which may represent modest oligomerization under both conditions. The size exclusion and TEM results are consistent with the ThT fluorescence indicating amyloid formation."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2016/06/14	2016/06/17	rogid:bWGG0F3GX4rZ3jlu2LxM1Q4TcXg8265	rogid:bWGG0F3GX4rZ3jlu2LxM1Q4TcXg8265	rigid:i+i/mj7QHm40+HdzFkhaVc30NKc	false	-	-	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)