#ID(s) interactor A ID(s) interactor B Alt. ID(s) interactor A Alt. ID(s) interactor B Alias(es) interactor A Alias(es) interactor B Interaction detection method(s) Publication 1st author(s) Publication Identifier(s) Taxid interactor A Taxid interactor B Interaction type(s) Source database(s) Interaction identifier(s) Confidence value(s) Expansion method(s) Biological role(s) interactor A Biological role(s) interactor B Experimental role(s) interactor A Experimental role(s) interactor B Type(s) interactor A Type(s) interactor B Xref(s) interactor A Xref(s) interactor B Interaction Xref(s) Annotation(s) interactor A Annotation(s) interactor B Interaction annotation(s) Host organism(s) Interaction parameter(s) Creation date Update date Checksum(s) interactor A Checksum(s) interactor B Interaction Checksum(s) Negative Feature(s) interactor A Feature(s) interactor B Stoichiometry(s) interactor A Stoichiometry(s) interactor B Identification method participant A Identification method participant B uniprotkb:D7PM04 uniprotkb:Q08121 intact:EBI-11666426 intact:EBI-11666418 psi-mi:d7pm04_clygr(display_long)|uniprotkb:GFP1(gene name)|psi-mi:GFP1(display_short) psi-mi:clyt_clygr(display_long)|uniprotkb:Phialidin(gene name synonym) psi-mi:"MI:0809"(bimolecular fluorescence complementation) Eremeeva et al. (2016) imex:IM-25065|pubmed:26867648 taxid:27801(Phialidium gregarium)|taxid:27801(Clytia gregaria) taxid:27801(Phialidium gregarium)|taxid:27801(Clytia gregaria) psi-mi:"MI:0407"(direct interaction) psi-mi:"MI:0471"(MINT) intact:EBI-11666436|imex:IM-25065-1 - - psi-mi:"MI:0919"(acceptor) psi-mi:"MI:0918"(donor) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) interpro:IPR009017(Green fluorescent protein-like)|interpro:IPR011584(Green fluorescent protein-related)|interpro:IPR000786(Green fluorescent protein, GFP)|go:"GO:0006091"(generation of precursor metabolites and energy)|go:"GO:0008218"(bioluminescence) interpro:IPR011992(EF-Hand type)|interpro:IPR018247|interpro:IPR002048(Calcium-binding EF-hand)|go:"GO:0005509"(calcium ion binding)|go:"GO:0008218"(bioluminescence) - - - figure legend:f1 f2 f3 f5 f6 t2|comment:In this work, we used the stopped-flow technique [29] to characterize the clytin/cgreGFP interaction. On the basis of the analysis of bioluminescence kinetics at different wavelengths, we propose that cgreGFP forms a transient complex with the reaction intermediates arising after Ca2+ binding with a photoprotein before the excited singlet state of product is formed.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2016/03/24 2016/03/24 rogid:dl8Iz+W/FkQRUtXpLwewNDgYvgM27801 rogid:epgVGkF2N/ctskoud4mKpphHNcU27801 rigid:ZmBchvruTLuDV3QaGlS6XHKueCg false - - - - psi-mi:"MI:0867"(tag visualisation by fluorescence) psi-mi:"MI:0867"(tag visualisation by fluorescence)