#ID(s) interactor A	ID(s) interactor B	Alt. ID(s) interactor A	Alt. ID(s) interactor B	Alias(es) interactor A	Alias(es) interactor B	Interaction detection method(s)	Publication 1st author(s)	Publication Identifier(s)	Taxid interactor A	Taxid interactor B	Interaction type(s)	Source database(s)	Interaction identifier(s)	Confidence value(s)	Expansion method(s)	Biological role(s) interactor A	Biological role(s) interactor B	Experimental role(s) interactor A	Experimental role(s) interactor B	Type(s) interactor A	Type(s) interactor B	Xref(s) interactor A	Xref(s) interactor B	Interaction Xref(s)	Annotation(s) interactor A	Annotation(s) interactor B	Interaction annotation(s)	Host organism(s)	Interaction parameter(s)	Creation date	Update date	Checksum(s) interactor A	Checksum(s) interactor B	Interaction Checksum(s)	Negative	Feature(s) interactor A	Feature(s) interactor B	Stoichiometry(s) interactor A	Stoichiometry(s) interactor B	Identification method participant A	Identification method participant B
uniprotkb:P0C053	uniprotkb:P0C053	intact:EBI-11666617|ensemblbacteria:BAF66494|uniprotkb:A0A0H3K6K4	intact:EBI-11666617|ensemblbacteria:BAF66494|uniprotkb:A0A0H3K6K4	psi-mi:essb_staae(display_long)|uniprotkb:NWMN_0222(locus name)|uniprotkb:essB(gene name)|psi-mi:essB(display_short)	psi-mi:essb_staae(display_long)|uniprotkb:NWMN_0222(locus name)|uniprotkb:essB(gene name)|psi-mi:essB(display_short)	psi-mi:"MI:0030"(cross-linking study)	Jäger et al. (2016)	imex:IM-25067|pubmed:26785823	taxid:426430(staae)|taxid:426430("Staphylococcus aureus (strain Newman)")	taxid:426430(staae)|taxid:426430("Staphylococcus aureus (strain Newman)")	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0471"(MINT)	intact:EBI-11666564|imex:IM-25067-1	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:WP_000240338.1|interpro:IPR018778|ensemblbacteria:BAF66494(transcript)|ensemblbacteria:NWMN_0222(gene)|go:"GO:0005886"(plasma membrane)|go:"GO:0016021"(integral component of membrane)|go:"GO:0042802"(identical protein binding)|interpro:IPR042565	refseq:WP_000240338.1|interpro:IPR018778|ensemblbacteria:BAF66494(transcript)|ensemblbacteria:NWMN_0222(gene)|go:"GO:0005886"(plasma membrane)|go:"GO:0016021"(integral component of membrane)|go:"GO:0042802"(identical protein binding)|interpro:IPR042565	-	comment:"In vivo crosslinking analysis of EssB resulted in no detectable EssB crosslinks unless the protein was overproduced, in which case a very faint crosslinked product running at the approximate size of an EssB homo-dimer could be detected (Fig 3B). As homo-dimerisation of EssB has been reported previously, [15, 16] it is likely that this crosslink is a homo-dimeric form of EssB."	comment:"In vivo crosslinking analysis of EssB resulted in no detectable EssB crosslinks unless the protein was overproduced, in which case a very faint crosslinked product running at the approximate size of an EssB homo-dimer could be detected (Fig 3B). As homo-dimerisation of EssB has been reported previously, [15, 16] it is likely that this crosslink is a homo-dimeric form of EssB."	figure legend:f2 f3|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2016/03/24	2016/03/30	rogid:HxCLQ9qJ8v0IQDBCeQmbp135sXo426430	rogid:HxCLQ9qJ8v0IQDBCeQmbp135sXo426430	rigid:9UC1P4yjgQ2teyZkmHHixc3G/6A	false	-	-	-	0	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)
uniprotkb:P0C049	uniprotkb:P0C049	intact:EBI-11666573|uniprotkb:A6QDR0|ensemblbacteria:BAF66492	intact:EBI-11666573|uniprotkb:A6QDR0|ensemblbacteria:BAF66492	psi-mi:esaa_staae(display_long)|uniprotkb:esaA(gene name)|psi-mi:esaA(display_short)|uniprotkb:NWMN_0220(locus name)	psi-mi:esaa_staae(display_long)|uniprotkb:esaA(gene name)|psi-mi:esaA(display_short)|uniprotkb:NWMN_0220(locus name)	psi-mi:"MI:0030"(cross-linking study)	Jäger et al. (2016)	imex:IM-25067|pubmed:26785823	taxid:426430(staae)|taxid:426430("Staphylococcus aureus (strain Newman)")	taxid:426430(staae)|taxid:426430("Staphylococcus aureus (strain Newman)")	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0471"(MINT)	intact:EBI-11666570|imex:IM-25067-3	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:WP_000728955.1|interpro:IPR023838|ensemblbacteria:BAF66492(transcript)|ensemblbacteria:NWMN_0220(gene)|go:"GO:0005886"(plasma membrane)|go:"GO:0016021"(integral component of membrane)|go:"GO:0042802"(identical protein binding)	refseq:WP_000728955.1|interpro:IPR023838|ensemblbacteria:BAF66492(transcript)|ensemblbacteria:NWMN_0220(gene)|go:"GO:0005886"(plasma membrane)|go:"GO:0016021"(integral component of membrane)|go:"GO:0042802"(identical protein binding)	-	-	-	figure legend:f3|comment:As shown in Fig. 3A, incubation with formaldehyde resulted in EsaA-specific crosslinks in whole cells of the wild-type strain. A series of 4–5 discrete bands could be detected, migrating around 250 kDa. Interestingly, essentially the same pattern of EsaA crosslinks was seen whether EssA, EssB or EssC were absent, suggesting that the crosslinked products did not contain any of these proteins. It has been reported that the long extracellular domain of YueB, the Bacillus subtilis homologue of EsaA, forms a highly elongated homo-dimer [17] and it is therefore likely that the crosslinks seen here correspond to different conformers of a crosslinked EsaA dimer.|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2016/03/24	2016/03/30	rogid:j+yxJlRU8uZvg4M3/cHRqFCrcvA426430	rogid:j+yxJlRU8uZvg4M3/cHRqFCrcvA426430	rigid:VvmPD4L+0lVCFic5nOk8ebP9LLM	false	-	-	-	0	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)
uniprotkb:P0C048	uniprotkb:P0C048	intact:EBI-11666588|uniprotkb:A6QDR3|ensemblbacteria:BAF66495	intact:EBI-11666588|uniprotkb:A6QDR3|ensemblbacteria:BAF66495	psi-mi:essc_staae(display_long)|uniprotkb:essC(gene name)|psi-mi:essC(display_short)|uniprotkb:NWMN_0223(locus name)	psi-mi:essc_staae(display_long)|uniprotkb:essC(gene name)|psi-mi:essC(display_short)|uniprotkb:NWMN_0223(locus name)	psi-mi:"MI:0030"(cross-linking study)	Jäger et al. (2016)	imex:IM-25067|pubmed:26785823	taxid:426430(staae)|taxid:426430("Staphylococcus aureus (strain Newman)")	taxid:426430(staae)|taxid:426430("Staphylococcus aureus (strain Newman)")	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0471"(MINT)	intact:EBI-11666606|imex:IM-25067-5	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:WP_000549278.1|interpro:IPR023839|interpro:IPR022206|interpro:IPR002543(Cell divisionFtsK/SpoIIIE)|interpro:IPR027417|interpro:IPR008984(SMAD/FHA domain)|ensemblbacteria:BAF66495(transcript)|ensemblbacteria:NWMN_0223(gene)|go:"GO:0003677"(DNA binding)|go:"GO:0005524"(ATP binding)|go:"GO:0005886"(plasma membrane)|go:"GO:0016021"(integral component of membrane)	refseq:WP_000549278.1|interpro:IPR023839|interpro:IPR022206|interpro:IPR002543(Cell divisionFtsK/SpoIIIE)|interpro:IPR027417|interpro:IPR008984(SMAD/FHA domain)|ensemblbacteria:BAF66495(transcript)|ensemblbacteria:NWMN_0223(gene)|go:"GO:0003677"(DNA binding)|go:"GO:0005524"(ATP binding)|go:"GO:0005886"(plasma membrane)|go:"GO:0016021"(integral component of membrane)	-	-	-	figure legend:f3|comment:"Crosslinking of EssC in vivo also yielded high molecular weight products, as was observed in vitro (Fig 3C). However, in contrast to the in vitro analysis, where one major crosslinked product was seen, in this instance at least two distinct high molecular weight species were detected, both of which migrated above the 460 kDa molecular weight marker. The differences between the pattern of crosslinks seen in vivo and in vitro may reflect the difference in size between DSS which was used as a crosslinker in vitro and formaldehyde which was used in vivo. Alternatively they may arise due to the presence of ATP, proton-motive force or other factors that are present in whole cells. It should be noted that as similar crosslinks were seen in the absence of any of EsaA, EssA or EssB, neither of the two crosslinks arise from interactions of EssC with any of the other core membrane components of the Ess system, and probably represent homo-multimeric forms of EssC."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2016/03/24	2016/03/30	rogid:n2UAEveHYWqxzHv09cTpUHV/E4s426430	rogid:n2UAEveHYWqxzHv09cTpUHV/E4s426430	rigid:e6jpZ/TEZ1gG/iTHLe2kEhLauDA	false	-	-	-	-	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)
uniprotkb:P0C053	uniprotkb:P0C053	intact:EBI-11666617|ensemblbacteria:BAF66494|uniprotkb:A0A0H3K6K4	intact:EBI-11666617|ensemblbacteria:BAF66494|uniprotkb:A0A0H3K6K4	psi-mi:essb_staae(display_long)|uniprotkb:NWMN_0222(locus name)|uniprotkb:essB(gene name)|psi-mi:essB(display_short)	psi-mi:essb_staae(display_long)|uniprotkb:NWMN_0222(locus name)|uniprotkb:essB(gene name)|psi-mi:essB(display_short)	psi-mi:"MI:0276"(blue native page)	Jäger et al. (2016)	imex:IM-25067|pubmed:26785823	taxid:426430(staae)|taxid:426430("Staphylococcus aureus (strain Newman)")	taxid:426430(staae)|taxid:426430("Staphylococcus aureus (strain Newman)")	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0471"(MINT)	intact:EBI-11666626|imex:IM-25067-7	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:WP_000240338.1|interpro:IPR018778|ensemblbacteria:BAF66494(transcript)|ensemblbacteria:NWMN_0222(gene)|go:"GO:0005886"(plasma membrane)|go:"GO:0016021"(integral component of membrane)|go:"GO:0042802"(identical protein binding)|interpro:IPR042565	refseq:WP_000240338.1|interpro:IPR018778|ensemblbacteria:BAF66494(transcript)|ensemblbacteria:NWMN_0222(gene)|go:"GO:0005886"(plasma membrane)|go:"GO:0016021"(integral component of membrane)|go:"GO:0042802"(identical protein binding)|interpro:IPR042565	-	-	-	figure legend:f5|comment:"However, bands containing EsaA and EssB were observed. As shown in Fig. 5(A), two complexes containing EsaA could be detected, one of which close to the 150 kDa marker and the second just above the 200 kDa marker, which could conceivably correspond to monomers and dimers of EsaA. In contrast, a single EssB-containing band was detected, migrating between the 66 kDa and 150 kDa marker, close to the expected size for an EssB dimer (Fig. 5B). Thus, given the differences in the masses of these bands these two proteins are highly unlikely to be part of the same complex. We confirmed this by repeating the BN PAGE on Fos-choline-12 membrane extracts from strains deleted for esaA or essB, and detected the same two EsaA complexes in the absence of EssB, and the same EssB complex in the absence of EsaA. We conclude that these two proteins do not interact with each other in isolated membranes."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2016/03/24	2016/03/30	rogid:HxCLQ9qJ8v0IQDBCeQmbp135sXo426430	rogid:HxCLQ9qJ8v0IQDBCeQmbp135sXo426430	rigid:9UC1P4yjgQ2teyZkmHHixc3G/6A	false	-	-	-	0	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)
uniprotkb:P0C049	uniprotkb:P0C049	intact:EBI-11666573|uniprotkb:A6QDR0|ensemblbacteria:BAF66492	intact:EBI-11666573|uniprotkb:A6QDR0|ensemblbacteria:BAF66492	psi-mi:esaa_staae(display_long)|uniprotkb:esaA(gene name)|psi-mi:esaA(display_short)|uniprotkb:NWMN_0220(locus name)	psi-mi:esaa_staae(display_long)|uniprotkb:esaA(gene name)|psi-mi:esaA(display_short)|uniprotkb:NWMN_0220(locus name)	psi-mi:"MI:0276"(blue native page)	Jäger et al. (2016)	imex:IM-25067|pubmed:26785823	taxid:426430(staae)|taxid:426430("Staphylococcus aureus (strain Newman)")	taxid:426430(staae)|taxid:426430("Staphylococcus aureus (strain Newman)")	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0471"(MINT)	intact:EBI-11666653|imex:IM-25067-9	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:WP_000728955.1|interpro:IPR023838|ensemblbacteria:BAF66492(transcript)|ensemblbacteria:NWMN_0220(gene)|go:"GO:0005886"(plasma membrane)|go:"GO:0016021"(integral component of membrane)|go:"GO:0042802"(identical protein binding)	refseq:WP_000728955.1|interpro:IPR023838|ensemblbacteria:BAF66492(transcript)|ensemblbacteria:NWMN_0220(gene)|go:"GO:0005886"(plasma membrane)|go:"GO:0016021"(integral component of membrane)|go:"GO:0042802"(identical protein binding)	-	-	-	figure legend:f5|comment:"However, bands containing EsaA and EssB were observed. As shown in Fig. 5(A), two complexes containing EsaA could be detected, one of which close to the 150 kDa marker and the second just above the 200 kDa marker, which could conceivably correspond to monomers and dimers of EsaA. In contrast, a single EssB-containing band was detected, migrating between the 66 kDa and 150 kDa marker, close to the expected size for an EssB dimer (Fig. 5B). Thus, given the differences in the masses of these bands these two proteins are highly unlikely to be part of the same complex. We confirmed this by repeating the BN PAGE on Fos-choline-12 membrane extracts from strains deleted for esaA or essB, and detected the same two EsaA complexes in the absence of EssB, and the same EssB complex in the absence of EsaA. We conclude that these two proteins do not interact with each other in isolated membranes."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2016/03/24	2016/03/30	rogid:j+yxJlRU8uZvg4M3/cHRqFCrcvA426430	rogid:j+yxJlRU8uZvg4M3/cHRqFCrcvA426430	rigid:VvmPD4L+0lVCFic5nOk8ebP9LLM	false	-	-	-	0	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)