#ID(s) interactor A	ID(s) interactor B	Alt. ID(s) interactor A	Alt. ID(s) interactor B	Alias(es) interactor A	Alias(es) interactor B	Interaction detection method(s)	Publication 1st author(s)	Publication Identifier(s)	Taxid interactor A	Taxid interactor B	Interaction type(s)	Source database(s)	Interaction identifier(s)	Confidence value(s)	Expansion method(s)	Biological role(s) interactor A	Biological role(s) interactor B	Experimental role(s) interactor A	Experimental role(s) interactor B	Type(s) interactor A	Type(s) interactor B	Xref(s) interactor A	Xref(s) interactor B	Interaction Xref(s)	Annotation(s) interactor A	Annotation(s) interactor B	Interaction annotation(s)	Host organism(s)	Interaction parameter(s)	Creation date	Update date	Checksum(s) interactor A	Checksum(s) interactor B	Interaction Checksum(s)	Negative	Feature(s) interactor A	Feature(s) interactor B	Stoichiometry(s) interactor A	Stoichiometry(s) interactor B	Identification method participant A	Identification method participant B
chebi:"CHEBI:91145"	uniprotkb:Q9Y251-PRO_0000042260	intact:EBI-11657951	intact:EBI-11600845	psi-mi:"alpha-d-glcnac-(1->4)-beta-d-glca-(1->4)-alpha-d-glcnac-(1->4)-beta-d-glca-o-pnp"(display_short)|psi-mi:"CHEBI:91145"(display_long)|intact:"alpha-D-GlcpNAc-(1->4)-c-GlcpA-(1->4)-alpha-D-GlcpNAc-(1->4)-beta-D-GlcpA-O-pNP"(synonym)|intact:"4-nitrophenyl alpha-D-N-acetylglucosaminyl-(1->4)-->-glucuronosyl-(1->4)-alpha-D-N-acetylglucosaminyl-(1->4)-->-glucuronic acid"(synonym)|intact:GlcNAc-GlcUA-GlcNAc-GlcUA-pNP(synonym)|intact:heparin oligosaccharide dp4, N-acetylated(synonym)|intact:"4-nitrophenyl 2-acetamido-2-deoxy-alpha-D-glucopyranosyl-(1->4)-->-glucopyranuronosyl-(1->4)-2-acetamido-2-deoxy-alpha-D-glucopyranosyl-(1->4)-->-glucopyranosiduronic acid"(iupac name)	psi-mi:q9y251-pro_0000042260(display_long)|uniprotkb:HPSE(gene name)|psi-mi:HPSE(display_short)|uniprotkb:HEP(gene name synonym)|uniprotkb:HPA(gene name synonym)|uniprotkb:HPA1(gene name synonym)|uniprotkb:HPR1(gene name synonym)|uniprotkb:HPSE1(gene name synonym)|uniprotkb:HSE1(gene name synonym)|uniprotkb:Endo-glucoronidase(gene name synonym)|uniprotkb:Heparanase-1(gene name synonym)	psi-mi:"MI:0114"(x-ray crystallography)	Wu et al. (2015)	imex:IM-24973|pubmed:26575439	taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)")	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0917"(matrixdb)	intact:EBI-11657948|wwpdb:5E97|imex:IM-24973-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0328"(small molecule)	psi-mi:"MI:0326"(protein)	-	intact:EBI-5453868(chain-parent)	-	-	chain-seq-start:36|chain-seq-end:109	figure legend:Figure 2b|3d-resolution:1.64 A|3d-r-factors:Rworking 17% Rfree 20%|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2016/01/22	2016/03/24	-	rogid:H5/+06r3zp/FcdQJUYkbHCS+hTA9606	-	false	-	-	-	1	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
chebi:"CHEBI:91145"	uniprotkb:Q9Y251-PRO_0000042262	intact:EBI-11657951	intact:EBI-11600831	psi-mi:"alpha-d-glcnac-(1->4)-beta-d-glca-(1->4)-alpha-d-glcnac-(1->4)-beta-d-glca-o-pnp"(display_short)|psi-mi:"CHEBI:91145"(display_long)|intact:"alpha-D-GlcpNAc-(1->4)-c-GlcpA-(1->4)-alpha-D-GlcpNAc-(1->4)-beta-D-GlcpA-O-pNP"(synonym)|intact:"4-nitrophenyl alpha-D-N-acetylglucosaminyl-(1->4)-->-glucuronosyl-(1->4)-alpha-D-N-acetylglucosaminyl-(1->4)-->-glucuronic acid"(synonym)|intact:GlcNAc-GlcUA-GlcNAc-GlcUA-pNP(synonym)|intact:heparin oligosaccharide dp4, N-acetylated(synonym)|intact:"4-nitrophenyl 2-acetamido-2-deoxy-alpha-D-glucopyranosyl-(1->4)-->-glucopyranuronosyl-(1->4)-2-acetamido-2-deoxy-alpha-D-glucopyranosyl-(1->4)-->-glucopyranosiduronic acid"(iupac name)	psi-mi:q9y251-pro_0000042262(display_long)|uniprotkb:HPSE(gene name)|psi-mi:HPSE(display_short)|uniprotkb:HEP(gene name synonym)|uniprotkb:HPA(gene name synonym)|uniprotkb:HPA1(gene name synonym)|uniprotkb:HPR1(gene name synonym)|uniprotkb:HPSE1(gene name synonym)|uniprotkb:HSE1(gene name synonym)|uniprotkb:Endo-glucoronidase(gene name synonym)|uniprotkb:Heparanase-1(gene name synonym)	psi-mi:"MI:0114"(x-ray crystallography)	Wu et al. (2015)	imex:IM-24973|pubmed:26575439	taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)")	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0917"(matrixdb)	intact:EBI-11657948|wwpdb:5E97|imex:IM-24973-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0328"(small molecule)	psi-mi:"MI:0326"(protein)	-	intact:EBI-5453868(chain-parent)	-	-	chain-seq-start:158|chain-seq-end:543	figure legend:Figure 2b|3d-resolution:1.64 A|3d-r-factors:Rworking 17% Rfree 20%|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2016/01/22	2016/03/24	-	rogid:32V76d1O9teLFL8sH2KSIs9uDJA9606	-	false	-	sufficient binding region:2-386	-	1	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
chebi:"CHEBI:91147"	uniprotkb:Q9Y251-PRO_0000042260	intact:EBI-11657962	intact:EBI-11600845	psi-mi:"alpha-d-glcns-(1->4)-beta-d-glca-(1->4)-alpha-d-glcns-(1->4)-beta-d-glca-o-pnp"(display_short)|psi-mi:"CHEBI:91147"(display_long)|intact:"4-nitrophenyl 2-deoxy-2-(sulfoamino)-alpha-D-glucosyl-(1->4)-beta-D-glucuronosyl-(1->4)-2-deoxy-2-(sulfoamino)-alpha-D-glucosyl-(1->4)-beta-D-glucuronic acid"(synonym)|intact:"alpha-D-GlcpNS-(1->4)-beta-D-GlcpA-(1->4)-alpha-D-GlcpNS-(1->4)-beta-D-GlcpA-O-pNP"(synonym)|intact:GlcNS-GlcUA-GlcNS-GlcUA-pNP(synonym)|intact:heparin tetrasaccharide, N-sulphated(synonym)|intact:"4-nitrophenyl 2-deoxy-2-(sulfoamino)-alpha-D-glucopyranosyl-(1->4)-beta-D-glucopyranuronosyl-(1->4)-2-deoxy-2-(sulfoamino)-alpha-D-glucopyranosyl-(1->4)-beta-D-glucopyranosiduronic acid"(iupac name)	psi-mi:q9y251-pro_0000042260(display_long)|uniprotkb:HPSE(gene name)|psi-mi:HPSE(display_short)|uniprotkb:HEP(gene name synonym)|uniprotkb:HPA(gene name synonym)|uniprotkb:HPA1(gene name synonym)|uniprotkb:HPR1(gene name synonym)|uniprotkb:HPSE1(gene name synonym)|uniprotkb:HSE1(gene name synonym)|uniprotkb:Endo-glucoronidase(gene name synonym)|uniprotkb:Heparanase-1(gene name synonym)	psi-mi:"MI:0114"(x-ray crystallography)	Wu et al. (2015)	imex:IM-24973|pubmed:26575439	taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)")	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0917"(matrixdb)	intact:EBI-11657972|wwpdb:5E98|imex:IM-24973-2	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0328"(small molecule)	psi-mi:"MI:0326"(protein)	-	intact:EBI-5453868(chain-parent)	-	-	chain-seq-start:36|chain-seq-end:109	figure legend:Figure 2c|3d-resolution:1.63 A|3d-r-factors:Rworking 17%, Rfree  20%|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2016/01/22	2016/04/20	-	rogid:H5/+06r3zp/FcdQJUYkbHCS+hTA9606	-	false	-	-	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
chebi:"CHEBI:91147"	uniprotkb:Q9Y251-PRO_0000042262	intact:EBI-11657962	intact:EBI-11600831	psi-mi:"alpha-d-glcns-(1->4)-beta-d-glca-(1->4)-alpha-d-glcns-(1->4)-beta-d-glca-o-pnp"(display_short)|psi-mi:"CHEBI:91147"(display_long)|intact:"4-nitrophenyl 2-deoxy-2-(sulfoamino)-alpha-D-glucosyl-(1->4)-beta-D-glucuronosyl-(1->4)-2-deoxy-2-(sulfoamino)-alpha-D-glucosyl-(1->4)-beta-D-glucuronic acid"(synonym)|intact:"alpha-D-GlcpNS-(1->4)-beta-D-GlcpA-(1->4)-alpha-D-GlcpNS-(1->4)-beta-D-GlcpA-O-pNP"(synonym)|intact:GlcNS-GlcUA-GlcNS-GlcUA-pNP(synonym)|intact:heparin tetrasaccharide, N-sulphated(synonym)|intact:"4-nitrophenyl 2-deoxy-2-(sulfoamino)-alpha-D-glucopyranosyl-(1->4)-beta-D-glucopyranuronosyl-(1->4)-2-deoxy-2-(sulfoamino)-alpha-D-glucopyranosyl-(1->4)-beta-D-glucopyranosiduronic acid"(iupac name)	psi-mi:q9y251-pro_0000042262(display_long)|uniprotkb:HPSE(gene name)|psi-mi:HPSE(display_short)|uniprotkb:HEP(gene name synonym)|uniprotkb:HPA(gene name synonym)|uniprotkb:HPA1(gene name synonym)|uniprotkb:HPR1(gene name synonym)|uniprotkb:HPSE1(gene name synonym)|uniprotkb:HSE1(gene name synonym)|uniprotkb:Endo-glucoronidase(gene name synonym)|uniprotkb:Heparanase-1(gene name synonym)	psi-mi:"MI:0114"(x-ray crystallography)	Wu et al. (2015)	imex:IM-24973|pubmed:26575439	taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)")	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0917"(matrixdb)	intact:EBI-11657972|wwpdb:5E98|imex:IM-24973-2	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0328"(small molecule)	psi-mi:"MI:0326"(protein)	-	intact:EBI-5453868(chain-parent)	-	-	chain-seq-start:158|chain-seq-end:543	figure legend:Figure 2c|3d-resolution:1.63 A|3d-r-factors:Rworking 17%, Rfree  20%|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2016/01/22	2016/04/20	-	rogid:32V76d1O9teLFL8sH2KSIs9uDJA9606	-	false	-	sufficient binding region:2-386	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
chebi:"CHEBI:91149"	uniprotkb:Q9Y251-PRO_0000042260	intact:EBI-11663946	intact:EBI-11600845	psi-mi:"beta-d-deltahexa(2s)-(1->4)-alpha-d-glcns(6s)-(1->4)-alpha-l-idoa-(1->4)-alpha-d-glcns(6s)"(display_short)|psi-mi:"CHEBI:91149"(display_long)|intact:"DeltaHexUA(2S)-GlcNS(6S)-IdoUA(2S)-GlcNS(6S)"(synonym)|intact:heparin-derived tetrasaccharide(synonym)|intact:"(5xi)-2-O-sulfo-beta-D-xylo-hexopyranuronosyl-(1->4)-2-deoxy-6-O-sulfo-2-(sulfoamino)-alpha-D-glucopyranosyl-(1->4)-2-O-sulfo-alpha-L-idopyranuronosyl-(1->4)-2-deoxy-6-O-sulfo-2-(sulfoamino)-alpha-D-glucopyranose"(iupac name)	psi-mi:q9y251-pro_0000042260(display_long)|uniprotkb:HPSE(gene name)|psi-mi:HPSE(display_short)|uniprotkb:HEP(gene name synonym)|uniprotkb:HPA(gene name synonym)|uniprotkb:HPA1(gene name synonym)|uniprotkb:HPR1(gene name synonym)|uniprotkb:HPSE1(gene name synonym)|uniprotkb:HSE1(gene name synonym)|uniprotkb:Endo-glucoronidase(gene name synonym)|uniprotkb:Heparanase-1(gene name synonym)	psi-mi:"MI:0114"(x-ray crystallography)	Wu et al. (2015)	imex:IM-24973|pubmed:26575439	taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)")	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0917"(matrixdb)	intact:EBI-11663954|wwpdb:5E9C|imex:IM-24973-3	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0328"(small molecule)	psi-mi:"MI:0326"(protein)	-	intact:EBI-5453868(chain-parent)	-	-	chain-seq-start:36|chain-seq-end:109	figure legend:Figure 2e|3d-resolution:1.73 A|3d-r-factors:Rworking 17% Rfree 21%|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2016/01/22	2016/04/20	-	rogid:H5/+06r3zp/FcdQJUYkbHCS+hTA9606	-	false	-	-	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
chebi:"CHEBI:91149"	uniprotkb:Q9Y251-PRO_0000042262	intact:EBI-11663946	intact:EBI-11600831	psi-mi:"beta-d-deltahexa(2s)-(1->4)-alpha-d-glcns(6s)-(1->4)-alpha-l-idoa-(1->4)-alpha-d-glcns(6s)"(display_short)|psi-mi:"CHEBI:91149"(display_long)|intact:"DeltaHexUA(2S)-GlcNS(6S)-IdoUA(2S)-GlcNS(6S)"(synonym)|intact:heparin-derived tetrasaccharide(synonym)|intact:"(5xi)-2-O-sulfo-beta-D-xylo-hexopyranuronosyl-(1->4)-2-deoxy-6-O-sulfo-2-(sulfoamino)-alpha-D-glucopyranosyl-(1->4)-2-O-sulfo-alpha-L-idopyranuronosyl-(1->4)-2-deoxy-6-O-sulfo-2-(sulfoamino)-alpha-D-glucopyranose"(iupac name)	psi-mi:q9y251-pro_0000042262(display_long)|uniprotkb:HPSE(gene name)|psi-mi:HPSE(display_short)|uniprotkb:HEP(gene name synonym)|uniprotkb:HPA(gene name synonym)|uniprotkb:HPA1(gene name synonym)|uniprotkb:HPR1(gene name synonym)|uniprotkb:HPSE1(gene name synonym)|uniprotkb:HSE1(gene name synonym)|uniprotkb:Endo-glucoronidase(gene name synonym)|uniprotkb:Heparanase-1(gene name synonym)	psi-mi:"MI:0114"(x-ray crystallography)	Wu et al. (2015)	imex:IM-24973|pubmed:26575439	taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)")	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0917"(matrixdb)	intact:EBI-11663954|wwpdb:5E9C|imex:IM-24973-3	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0328"(small molecule)	psi-mi:"MI:0326"(protein)	-	intact:EBI-5453868(chain-parent)	-	-	chain-seq-start:158|chain-seq-end:543	figure legend:Figure 2e|3d-resolution:1.73 A|3d-r-factors:Rworking 17% Rfree 21%|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2016/01/22	2016/04/20	-	rogid:32V76d1O9teLFL8sH2KSIs9uDJA9606	-	false	-	sufficient binding region:2-386	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
chebi:"CHEBI:91148"	uniprotkb:Q9Y251-PRO_0000042260	intact:EBI-11664424	intact:EBI-11600845	psi-mi:heparin nonasaccharide(display_short)|psi-mi:"CHEBI:91148"(display_long)|intact:"beta-D-GlcA-(1->4)-alpha-D-GlcNS-(1->4)-beta-D-GlcA-(1->4)-alpha-D-GlcNS-(1->4)-beta-D-GlcA-(1->4)-alpha-D-GlcNS-(1->4)-beta-D-GlcA-(1->4)-alpha-D-GlcNS(6S)-(1->4)-beta-D-GlcA-O-pNP"(synonym)	psi-mi:q9y251-pro_0000042260(display_long)|uniprotkb:HPSE(gene name)|psi-mi:HPSE(display_short)|uniprotkb:HEP(gene name synonym)|uniprotkb:HPA(gene name synonym)|uniprotkb:HPA1(gene name synonym)|uniprotkb:HPR1(gene name synonym)|uniprotkb:HPSE1(gene name synonym)|uniprotkb:HSE1(gene name synonym)|uniprotkb:Endo-glucoronidase(gene name synonym)|uniprotkb:Heparanase-1(gene name synonym)	psi-mi:"MI:0114"(x-ray crystallography)	Wu et al. (2015)	imex:IM-24973|pubmed:26575439	taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)")	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0917"(matrixdb)	intact:EBI-11664425|wwpdb:5E9B|imex:IM-24973-4	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0328"(small molecule)	psi-mi:"MI:0326"(protein)	chebi:"CHEBI:91148"(HepMer_dp09_0001)	intact:EBI-5453868(chain-parent)	-	-	chain-seq-start:36|chain-seq-end:109	figure legend:Figure 2d|3d-resolution:1.88 A|3d-r-factors:Rworking 18% Rfree 22%|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2016/01/22	2019/09/04	-	rogid:H5/+06r3zp/FcdQJUYkbHCS+hTA9606	-	false	-	-	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
chebi:"CHEBI:91148"	uniprotkb:Q9Y251-PRO_0000042262	intact:EBI-11664424	intact:EBI-11600831	psi-mi:heparin nonasaccharide(display_short)|psi-mi:"CHEBI:91148"(display_long)|intact:"beta-D-GlcA-(1->4)-alpha-D-GlcNS-(1->4)-beta-D-GlcA-(1->4)-alpha-D-GlcNS-(1->4)-beta-D-GlcA-(1->4)-alpha-D-GlcNS-(1->4)-beta-D-GlcA-(1->4)-alpha-D-GlcNS(6S)-(1->4)-beta-D-GlcA-O-pNP"(synonym)	psi-mi:q9y251-pro_0000042262(display_long)|uniprotkb:HPSE(gene name)|psi-mi:HPSE(display_short)|uniprotkb:HEP(gene name synonym)|uniprotkb:HPA(gene name synonym)|uniprotkb:HPA1(gene name synonym)|uniprotkb:HPR1(gene name synonym)|uniprotkb:HPSE1(gene name synonym)|uniprotkb:HSE1(gene name synonym)|uniprotkb:Endo-glucoronidase(gene name synonym)|uniprotkb:Heparanase-1(gene name synonym)	psi-mi:"MI:0114"(x-ray crystallography)	Wu et al. (2015)	imex:IM-24973|pubmed:26575439	taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)")	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0917"(matrixdb)	intact:EBI-11664425|wwpdb:5E9B|imex:IM-24973-4	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0328"(small molecule)	psi-mi:"MI:0326"(protein)	chebi:"CHEBI:91148"(HepMer_dp09_0001)	intact:EBI-5453868(chain-parent)	-	-	chain-seq-start:158|chain-seq-end:543	figure legend:Figure 2d|3d-resolution:1.88 A|3d-r-factors:Rworking 18% Rfree 22%|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2016/01/22	2019/09/04	-	rogid:32V76d1O9teLFL8sH2KSIs9uDJA9606	-	false	-	sufficient binding region:2-386	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
chebi:"CHEBI:91148"	intenz:3.2.1.166	intact:EBI-11664424	intact:EBI-11600820|reactome:R-HSA-1666976|wwpdb:5e98|wwpdb:5e9b|wwpdb:5e9c|wwpdb:5e97|wwpdb:5e8m	psi-mi:heparin nonasaccharide(display_short)|psi-mi:"CHEBI:91148"(display_long)|intact:"beta-D-GlcA-(1->4)-alpha-D-GlcNS-(1->4)-beta-D-GlcA-(1->4)-alpha-D-GlcNS-(1->4)-beta-D-GlcA-(1->4)-alpha-D-GlcNS-(1->4)-beta-D-GlcA-(1->4)-alpha-D-GlcNS(6S)-(1->4)-beta-D-GlcA-O-pNP"(synonym)	psi-mi:hpse_human-1(display_short)|psi-mi:3.2.1.166(display_long)|intact:HPA1 complex(complex synonym)|intact:Heparanase complex(complex recommended name)|intact:HPSE dimer(complex systematic name)	psi-mi:"MI:0990"(cleavage assay)	Wu et al. (2015)	imex:IM-24973|pubmed:26575439	taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)")	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0194"(cleavage reaction)	psi-mi:"MI:0917"(matrixdb)	intact:EBI-11665893|imex:IM-24973-5	-	-	psi-mi:"MI:0501"(enzyme)	psi-mi:"MI:0502"(enzyme target)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0328"(small molecule)	psi-mi:"MI:1302"(stable complex)	-	efo:"EFO:0000668"(see-also)|efo:"EFO:0000311"(see-also)|go:"GO:0005776"(autophagosome)|go:"GO:1900026"(positive regulation of substrate adhesion-dependent cell spreading)|go:"GO:0030194"(positive regulation of blood coagulation)|pubmed:25732677(see-also)|go:"GO:0006954"(inflammatory response)|go:"GO:0030335"(positive regulation of cell migration)|go:"GO:0002542"(Factor XII activation)|go:"GO:1904974"(heparanase complex)|go:"GO:0030200"(heparan sulfate proteoglycan catabolic process)|go:"GO:0008284"(positive regulation of cell population proliferation)|go:"GO:0045766"(positive regulation of angiogenesis)|go:"GO:0001954"(positive regulation of cell-matrix adhesion)|go:"GO:0005768"(endosome)|go:"GO:0005576"(extracellular region)|go:"GO:0090091"(positive regulation of extracellular matrix disassembly)|go:"GO:0030305"(heparanase activity)|go:"GO:0043395"(heparan sulfate proteoglycan binding)|go:"GO:0010508"(positive regulation of autophagy)|evidence ontology:"ECO:0000353"|complex portal:CPX-362(complex-primary)	-	-	curated-complex:"An endo-beta-D-glucuronidase of the glycoside hydrolase 79 (GH79) family complex that cleaves the extracellular matrix heparan sulfate proteoglycans (HSPGs) into HS side chains and core proteoglycans. Selectively cleaves the linkage between a glucuronic acid unit and an N-sulfo glucosamine unit of HSPGs carrying either a 3-O-sulfo, a 6-O-sulfo or a 2-O-sulfo group, but not linkages between a glucuronic acid unit and a 2-O-sulfated iduronic acid moiety. HS sulfation serves as a molecular signal that directs heparanase to cleave only certain glycan sites and acts as mechanistic handles by which the enzyme can prize open the substrate HS helix and more effectively access the trisaccharide cleavage site via heparanase-binding induced distortion of the substrate helix.     Present in a variety of cellular locations where it performs an essential housekeeping role in catabolic processing of internalized HSPGs and at the cell surface or released into the ECM where it participates in ECM degradation and remodeling.     HSPGs ensure that bioactive molecules such as growth factors, chemokines, lipoproteins, and enzymes are localized to the cell surface and ECM and function in the control of normal and pathological processes. These include morphogenesis, osteogenesis, hair follicle inner root sheath differentiation and hair homeostasis, wound healing, shedding of syndecans, inflammation, pre-eclampsia, autoimmunity, cell proliferation and migration associated with metastasis, endothelial invasion and angiogenesis. Cleavage of HSPGs is likely to release these regulators that can alter the functional state of tissues and provide a mechanism by which cells can respond rapidly to changes in the extracellular environment. The proliferative advantages conferred by heparanase lead to its up-regulation in tumors in a variety of tissues, and its over-expression correlates strongly with metastasis and worsened clinical prognoses.     Also acts as pro-coagulant by increasing the generation of activation factor X in the presence of tissue factor and activation factor VII. Increases cell adhesion to the extracellular matrix (ECM) independent of its enzymatic activity.    HPSE is the only mammalian gene so far identified to have heparanase activity."|complex-properties:Secreted as a latent enzyme and internalized into late endosomes or lysosomes where it is subjected to proteolytic cleavage and activation. Cleavage at Glu109-Ser110 and Gln157-Lys158 yields an N-terminal 8 kDa and a C-terminal 50 kDa polypeptide that combine to form the catalytic site. Subsequent exocytosis transports the heparanase to its target sites.     Prior to cleavage, the linker between the the 8 kD and 50 kD proteolytic chains acts as a steric block.     Residues Glu-225 and Glu-343, located in the catalytic cleft, are crucial for its heparanase activity having been identified as the catalytic nucleophile and acid-base of HPSE.|complex-assembly:Heterodimer|antagonist:"heparin (CHEBI:28304)"|agonist:"calcium atom (CHEBI:22984)"|agonist:"magnesium atom (CHEBI:25107)"|disease:"Cancer [EFO:0000311]: upregulation of heparanase enhances tumor progression, metastatic development and cellular invasion in a variety of cancers."|disease:"Pre-eclampsia [EFO:0000668]: A complication of pregnancy, characterized by a complex of symptoms including maternal hypertension and proteinuria with or without pathological edema. Symptoms may range between mild and severe. Pre-eclampsia usually occurs after the 20th week of gestation, but may develop before this time in the presence of trophoblastic disease."|curation request:Sylvie Ricard-Blum, MatrixDB, Lyon	figure legend:Figure 3S|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	kcat:0.53x1^1 ~0.02(second -1)|km:7.7x10^-6 ~1.42(molar)	2016/01/22	2019/09/04	-	-	-	false	-	-	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)