#ID(s) interactor A	ID(s) interactor B	Alt. ID(s) interactor A	Alt. ID(s) interactor B	Alias(es) interactor A	Alias(es) interactor B	Interaction detection method(s)	Publication 1st author(s)	Publication Identifier(s)	Taxid interactor A	Taxid interactor B	Interaction type(s)	Source database(s)	Interaction identifier(s)	Confidence value(s)	Expansion method(s)	Biological role(s) interactor A	Biological role(s) interactor B	Experimental role(s) interactor A	Experimental role(s) interactor B	Type(s) interactor A	Type(s) interactor B	Xref(s) interactor A	Xref(s) interactor B	Interaction Xref(s)	Annotation(s) interactor A	Annotation(s) interactor B	Interaction annotation(s)	Host organism(s)	Interaction parameter(s)	Creation date	Update date	Checksum(s) interactor A	Checksum(s) interactor B	Interaction Checksum(s)	Negative	Feature(s) interactor A	Feature(s) interactor B	Stoichiometry(s) interactor A	Stoichiometry(s) interactor B	Identification method participant A	Identification method participant B
uniprotkb:Q06551	uniprotkb:P41912	intact:EBI-37230|uniprotkb:Q6B217|uniprotkb:D6VYP4|ensemblfungi:YLR246W	intact:EBI-2087870|uniprotkb:D6W1V7|ensemblfungi:YOL110W	psi-mi:erfb_yeast(display_long)|uniprotkb:DHHC cysteine-rich domain-containing protein ERF2(gene name synonym)|uniprotkb:Ras protein acyltransferase(gene name synonym)|uniprotkb:ERF2(gene name)|psi-mi:ERF2(display_short)|uniprotkb:YLR246W(locus name)	psi-mi:erfd_yeast(display_long)|uniprotkb:YOL110W(locus name)|uniprotkb:HRC237(orf name)|uniprotkb:SHR5(gene name)|psi-mi:SHR5(display_short)|uniprotkb:ERF4(gene name synonym)|uniprotkb:Hyperactive Ras suppressor protein 5(gene name synonym)	psi-mi:"MI:0096"(pull down)	Lobo et al. (2002)	pubmed:12193598|imex:IM-25640	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-12556304|imex:IM-25640-1	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_013347.1|sgd:S000004236|mint:Q06551|dip:DIP-4783N|ensemblfungi:YLR246W(gene)|ensemblfungi:YLR246W_mRNA(transcript)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0006612"(protein targeting to membrane)|go:"GO:0016409"(palmitoyltransferase activity)|go:"GO:0018230"(peptidyl-L-cysteine S-palmitoylation)|go:"GO:0018345"(protein palmitoylation)|go:"GO:0019706"(protein-cysteine S-palmitoyltransferase activity)|go:"GO:0030176"(integral component of endoplasmic reticulum membrane)|go:"GO:0031211"(endoplasmic reticulum palmitoyltransferase complex)|go:"GO:0032541"(cortical endoplasmic reticulum)|go:"GO:0097038"(perinuclear endoplasmic reticulum)|interpro:IPR001594(Zinc finger, DHHC-type)	refseq:NP_014531.1|sgd:S000005470|dip:DIP-7228N|mint:P41912|ensemblfungi:YOL110W(gene)|ensemblfungi:YOL110W_mRNA(transcript)|go:"GO:0002178"(palmitoyltransferase complex)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0006612"(protein targeting to membrane)|go:"GO:0018230"(peptidyl-L-cysteine S-palmitoylation)|go:"GO:0018345"(protein palmitoylation)|go:"GO:0031211"(endoplasmic reticulum palmitoyltransferase complex)|go:"GO:0042406"(extrinsic component of endoplasmic reticulum membrane)|interpro:IPR019383|reactome:R-SCE-6798695	-	-	-	figure legend:Fig. 1B, 2|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	-	2016/10/06	2016/10/07	rogid:6LBri1qWafIHxliiafTXrbrZ0JQ559292	rogid:BQ1Foh34E0ylxN2XnzqzluZradc559292	rigid:fuE2HDCyE0x9G5b2DzCsg9KSBZA	false	flag tag:n-n|mutation disrupting interaction strength:189-189|mutation with no effect:201-201	glutathione s tranferase tag:n-n	-	-	psi-mi:"MI:0705"(anti tag western blot)	psi-mi:"MI:0705"(anti tag western blot)
chebi:"CHEBI:15525"	intenz:2.3.1.225	intact:EBI-2008688	intact:EBI-12556324	psi-mi:palmitoyl-coa(display_short)|psi-mi:"CHEBI:15525"(display_long)	psi-mi:erf2-shr5-1(display_short)|psi-mi:2.3.1.225(display_long)|intact:Palmitoyltransferase ERF2/SHR5 complex(complex recommended name)|intact:"ERF2:SHR5"(complex systematic name)|intact:Erf2/Erf4 PAT complex(complex synonym)|intact:Palmitoyltransferase ERF2-SHR5 complex(complex synonym)|intact:Erf2-Erf4 PAT complex(complex synonym)	psi-mi:"MI:1004"(palmitoylase assay)	Lobo et al. (2002)	pubmed:12193598|imex:IM-25640	taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)")	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	psi-mi:"MI:0216"(palmitoylation reaction)	psi-mi:"MI:0469"(IntAct)	intact:EBI-12556567|imex:IM-25640-2	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0502"(enzyme target)	psi-mi:"MI:0501"(enzyme)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0328"(small molecule)	psi-mi:"MI:1302"(stable complex)	-	pubmed:20851885(see-also)|pubmed:10490616(see-also)|pubmed:12193598(see-also)|intact:EBI-12556304(exp-evidence)|go:"GO:0018230"(peptidyl-L-cysteine S-palmitoylation)|go:"GO:0031211"(endoplasmic reticulum palmitoyltransferase complex)|go:"GO:0030176"(integral component of endoplasmic reticulum membrane)|go:"GO:0019706"(protein-cysteine S-palmitoyltransferase activity)|evidence ontology:"ECO:0000353"|complex portal:CPX-813(complex-primary)	go:"GO:0019706"(protein-cysteine S-palmitoyltransferase activity)	-	curated-complex:Palmitoyltransferase complex that catalyzes the palmitoylation of proteins through a two step reaction - the autopalmitoylation of the enzyme to create a palmitoyl-ERF2 intermediate followed by the transfer of the palmitoyl moiety to the RAS substrate. Palmitoyl-CoA serves as the palmitate donor.|complex-properties:ERF2 appears to have a direct role in palmitate transfer based on its ability to form an acyl-intermediate.	figure legend:Fig. 1C, Fig. 4|comment:Erf2 Cys189Ser and His201Ala mutants abolished the catalytic activity of the enzyme complex|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2016/10/06	2016/10/07	-	-	-	false	3h radiolabel:?-?	glutathione s tranferase tag:n-n|flag tag:n-n	-	-	psi-mi:"MI:0866"(tag visualisation)	psi-mi:"MI:0866"(tag visualisation)
intenz:2.3.1.225	uniprotkb:P01120	intact:EBI-12556324	intact:EBI-14838|uniprotkb:Q45U01|uniprotkb:D6W181|ensemblfungi:YNL098C	psi-mi:erf2-shr5-1(display_short)|psi-mi:2.3.1.225(display_long)|intact:Palmitoyltransferase ERF2/SHR5 complex(complex recommended name)|intact:"ERF2:SHR5"(complex systematic name)|intact:Erf2/Erf4 PAT complex(complex synonym)|intact:Palmitoyltransferase ERF2-SHR5 complex(complex synonym)|intact:Erf2-Erf4 PAT complex(complex synonym)	psi-mi:ras2_yeast(display_long)|uniprotkb:RAS2(gene name)|psi-mi:RAS2(display_short)|uniprotkb:ASC1(gene name synonym)|uniprotkb:CTN5(gene name synonym)|uniprotkb:GLC5(gene name synonym)|uniprotkb:YNL098C(locus name)|uniprotkb:N2198(orf name)	psi-mi:"MI:1004"(palmitoylase assay)	Lobo et al. (2002)	pubmed:12193598|imex:IM-25640	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	psi-mi:"MI:0216"(palmitoylation reaction)	psi-mi:"MI:0469"(IntAct)	intact:EBI-12556567|imex:IM-25640-2	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0501"(enzyme)	psi-mi:"MI:0502"(enzyme target)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:1302"(stable complex)	psi-mi:"MI:0326"(protein)	pubmed:20851885(see-also)|pubmed:10490616(see-also)|pubmed:12193598(see-also)|intact:EBI-12556304(exp-evidence)|go:"GO:0018230"(peptidyl-L-cysteine S-palmitoylation)|go:"GO:0031211"(endoplasmic reticulum palmitoyltransferase complex)|go:"GO:0030176"(integral component of endoplasmic reticulum membrane)|go:"GO:0019706"(protein-cysteine S-palmitoyltransferase activity)|evidence ontology:"ECO:0000353"|complex portal:CPX-813(complex-primary)	refseq:NP_014301.1|sgd:S000005042|go:"GO:0032880"(regulation of protein localization)|go:"GO:0045762"(positive regulation of adenylate cyclase activity)|go:"GO:0071944"(cell periphery)|go:"GO:0097271"(protein localization to bud neck)|go:"GO:2000222"(positive regulation of pseudohyphal growth)|interpro:IPR001806(Ras GTPase)|interpro:IPR005225(Small GTP-binding protein)|interpro:IPR020849|interpro:IPR027417|reactome:R-SCE-9696273|go:"GO:0010603"(regulation of cytoplasmic mRNA processing body assembly)|go:"GO:0016236"(macroautophagy)|go:"GO:0019003"(GDP binding)|go:"GO:0030437"(ascospore formation)|go:"GO:0032258"(cytoplasm to vacuole transport by the Cvt pathway)|ensemblfungi:YNL098C(gene)|ensemblfungi:YNL098C_mRNA(transcript)|go:"GO:0000411"(positive regulation of transcription by galactose)|go:"GO:0003924"(GTPase activity)|go:"GO:0003925"(G protein activity)|go:"GO:0005525"(GTP binding)|go:"GO:0005634"(nucleus)|go:"GO:0005739"(mitochondrion)|go:"GO:0005789"(endoplasmic reticulum membrane)|go:"GO:0005886"(plasma membrane)|go:"GO:0007190"(activation of adenylate cyclase activity)|go:"GO:0007265"(Ras protein signal transduction)|dip:DIP-2263N|mint:P01120	go:"GO:0019706"(protein-cysteine S-palmitoyltransferase activity)	curated-complex:Palmitoyltransferase complex that catalyzes the palmitoylation of proteins through a two step reaction - the autopalmitoylation of the enzyme to create a palmitoyl-ERF2 intermediate followed by the transfer of the palmitoyl moiety to the RAS substrate. Palmitoyl-CoA serves as the palmitate donor.|complex-properties:ERF2 appears to have a direct role in palmitate transfer based on its ability to form an acyl-intermediate.	crc64:6CAAAC531EEAE92F	figure legend:Fig. 1C, Fig. 4|comment:Erf2 Cys189Ser and His201Ala mutants abolished the catalytic activity of the enzyme complex|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2016/10/06	2016/10/07	-	rogid:MJS2Zuw6xxrxaLE6r3omwm+2vm4559292	-	false	glutathione s tranferase tag:n-n|flag tag:n-n	glutathione s tranferase tag:?-?	-	-	psi-mi:"MI:0866"(tag visualisation)	psi-mi:"MI:0866"(tag visualisation)
chebi:"CHEBI:15525"	intenz:2.3.1.225	intact:EBI-2008688	intact:EBI-12556324	psi-mi:palmitoyl-coa(display_short)|psi-mi:"CHEBI:15525"(display_long)	psi-mi:erf2-shr5-1(display_short)|psi-mi:2.3.1.225(display_long)|intact:Palmitoyltransferase ERF2/SHR5 complex(complex recommended name)|intact:"ERF2:SHR5"(complex systematic name)|intact:Erf2/Erf4 PAT complex(complex synonym)|intact:Palmitoyltransferase ERF2-SHR5 complex(complex synonym)|intact:Erf2-Erf4 PAT complex(complex synonym)	psi-mi:"MI:1004"(palmitoylase assay)	Lobo et al. (2002)	pubmed:12193598|imex:IM-25640	taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)")	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	psi-mi:"MI:0216"(palmitoylation reaction)	psi-mi:"MI:0469"(IntAct)	intact:EBI-12556624|imex:IM-25640-4	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0502"(enzyme target)	psi-mi:"MI:0501"(enzyme)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0328"(small molecule)	psi-mi:"MI:1302"(stable complex)	-	pubmed:20851885(see-also)|pubmed:10490616(see-also)|pubmed:12193598(see-also)|intact:EBI-12556304(exp-evidence)|go:"GO:0018230"(peptidyl-L-cysteine S-palmitoylation)|go:"GO:0031211"(endoplasmic reticulum palmitoyltransferase complex)|go:"GO:0030176"(integral component of endoplasmic reticulum membrane)|go:"GO:0019706"(protein-cysteine S-palmitoyltransferase activity)|evidence ontology:"ECO:0000353"|complex portal:CPX-813(complex-primary)	go:"GO:0019706"(protein-cysteine S-palmitoyltransferase activity)	-	curated-complex:Palmitoyltransferase complex that catalyzes the palmitoylation of proteins through a two step reaction - the autopalmitoylation of the enzyme to create a palmitoyl-ERF2 intermediate followed by the transfer of the palmitoyl moiety to the RAS substrate. Palmitoyl-CoA serves as the palmitate donor.|complex-properties:ERF2 appears to have a direct role in palmitate transfer based on its ability to form an acyl-intermediate.	figure legend:Fig. 3B|comment:Erf2 Cys189Ser and His201Ala mtants abolished the catalytic activity of the enzyme complex|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2016/10/06	2016/10/07	-	-	-	false	3h radiolabel:?-?	glutathione s tranferase tag:n-n|flag tag:n-n	-	-	psi-mi:"MI:0866"(tag visualisation)	psi-mi:"MI:0866"(tag visualisation)
intenz:2.3.1.225	uniprotkb:P01120	intact:EBI-12556324	intact:EBI-14838|uniprotkb:Q45U01|uniprotkb:D6W181|ensemblfungi:YNL098C	psi-mi:erf2-shr5-1(display_short)|psi-mi:2.3.1.225(display_long)|intact:Palmitoyltransferase ERF2/SHR5 complex(complex recommended name)|intact:"ERF2:SHR5"(complex systematic name)|intact:Erf2/Erf4 PAT complex(complex synonym)|intact:Palmitoyltransferase ERF2-SHR5 complex(complex synonym)|intact:Erf2-Erf4 PAT complex(complex synonym)	psi-mi:ras2_yeast(display_long)|uniprotkb:RAS2(gene name)|psi-mi:RAS2(display_short)|uniprotkb:ASC1(gene name synonym)|uniprotkb:CTN5(gene name synonym)|uniprotkb:GLC5(gene name synonym)|uniprotkb:YNL098C(locus name)|uniprotkb:N2198(orf name)	psi-mi:"MI:1004"(palmitoylase assay)	Lobo et al. (2002)	pubmed:12193598|imex:IM-25640	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	psi-mi:"MI:0216"(palmitoylation reaction)	psi-mi:"MI:0469"(IntAct)	intact:EBI-12556624|imex:IM-25640-4	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0501"(enzyme)	psi-mi:"MI:0502"(enzyme target)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:1302"(stable complex)	psi-mi:"MI:0326"(protein)	pubmed:20851885(see-also)|pubmed:10490616(see-also)|pubmed:12193598(see-also)|intact:EBI-12556304(exp-evidence)|go:"GO:0018230"(peptidyl-L-cysteine S-palmitoylation)|go:"GO:0031211"(endoplasmic reticulum palmitoyltransferase complex)|go:"GO:0030176"(integral component of endoplasmic reticulum membrane)|go:"GO:0019706"(protein-cysteine S-palmitoyltransferase activity)|evidence ontology:"ECO:0000353"|complex portal:CPX-813(complex-primary)	refseq:NP_014301.1|sgd:S000005042|go:"GO:0032880"(regulation of protein localization)|go:"GO:0045762"(positive regulation of adenylate cyclase activity)|go:"GO:0071944"(cell periphery)|go:"GO:0097271"(protein localization to bud neck)|go:"GO:2000222"(positive regulation of pseudohyphal growth)|interpro:IPR001806(Ras GTPase)|interpro:IPR005225(Small GTP-binding protein)|interpro:IPR020849|interpro:IPR027417|reactome:R-SCE-9696273|go:"GO:0010603"(regulation of cytoplasmic mRNA processing body assembly)|go:"GO:0016236"(macroautophagy)|go:"GO:0019003"(GDP binding)|go:"GO:0030437"(ascospore formation)|go:"GO:0032258"(cytoplasm to vacuole transport by the Cvt pathway)|ensemblfungi:YNL098C(gene)|ensemblfungi:YNL098C_mRNA(transcript)|go:"GO:0000411"(positive regulation of transcription by galactose)|go:"GO:0003924"(GTPase activity)|go:"GO:0003925"(G protein activity)|go:"GO:0005525"(GTP binding)|go:"GO:0005634"(nucleus)|go:"GO:0005739"(mitochondrion)|go:"GO:0005789"(endoplasmic reticulum membrane)|go:"GO:0005886"(plasma membrane)|go:"GO:0007190"(activation of adenylate cyclase activity)|go:"GO:0007265"(Ras protein signal transduction)|dip:DIP-2263N|mint:P01120	go:"GO:0019706"(protein-cysteine S-palmitoyltransferase activity)	curated-complex:Palmitoyltransferase complex that catalyzes the palmitoylation of proteins through a two step reaction - the autopalmitoylation of the enzyme to create a palmitoyl-ERF2 intermediate followed by the transfer of the palmitoyl moiety to the RAS substrate. Palmitoyl-CoA serves as the palmitate donor.|complex-properties:ERF2 appears to have a direct role in palmitate transfer based on its ability to form an acyl-intermediate.	crc64:6CAAAC531EEAE92F	figure legend:Fig. 3B|comment:Erf2 Cys189Ser and His201Ala mtants abolished the catalytic activity of the enzyme complex|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2016/10/06	2016/10/07	-	rogid:MJS2Zuw6xxrxaLE6r3omwm+2vm4559292	-	false	glutathione s tranferase tag:n-n|flag tag:n-n	glutathione s tranferase tag:?-?	-	-	psi-mi:"MI:0866"(tag visualisation)	psi-mi:"MI:0866"(tag visualisation)
chebi:"CHEBI:15525"	intenz:2.3.1.225	intact:EBI-2008688	intact:EBI-12556324	psi-mi:palmitoyl-coa(display_short)|psi-mi:"CHEBI:15525"(display_long)	psi-mi:erf2-shr5-1(display_short)|psi-mi:2.3.1.225(display_long)|intact:Palmitoyltransferase ERF2/SHR5 complex(complex recommended name)|intact:"ERF2:SHR5"(complex systematic name)|intact:Erf2/Erf4 PAT complex(complex synonym)|intact:Palmitoyltransferase ERF2-SHR5 complex(complex synonym)|intact:Erf2-Erf4 PAT complex(complex synonym)	psi-mi:"MI:1004"(palmitoylase assay)	Lobo et al. (2002)	pubmed:12193598|imex:IM-25640	taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)")	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	psi-mi:"MI:0216"(palmitoylation reaction)	psi-mi:"MI:0469"(IntAct)	intact:EBI-13637798|imex:IM-25640-5	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0502"(enzyme target)	psi-mi:"MI:0501"(enzyme)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0328"(small molecule)	psi-mi:"MI:1302"(stable complex)	-	pubmed:20851885(see-also)|pubmed:10490616(see-also)|pubmed:12193598(see-also)|intact:EBI-12556304(exp-evidence)|go:"GO:0018230"(peptidyl-L-cysteine S-palmitoylation)|go:"GO:0031211"(endoplasmic reticulum palmitoyltransferase complex)|go:"GO:0030176"(integral component of endoplasmic reticulum membrane)|go:"GO:0019706"(protein-cysteine S-palmitoyltransferase activity)|evidence ontology:"ECO:0000353"|complex portal:CPX-813(complex-primary)	go:"GO:0019706"(protein-cysteine S-palmitoyltransferase activity)	-	curated-complex:Palmitoyltransferase complex that catalyzes the palmitoylation of proteins through a two step reaction - the autopalmitoylation of the enzyme to create a palmitoyl-ERF2 intermediate followed by the transfer of the palmitoyl moiety to the RAS substrate. Palmitoyl-CoA serves as the palmitate donor.|complex-properties:ERF2 appears to have a direct role in palmitate transfer based on its ability to form an acyl-intermediate.	figure legend:Table 1|kinetics:PAT activity 2720 pmol palmitate transferred/min/μmol substrate|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2016/10/06	2017/01/26	-	-	-	false	3h radiolabel:?-?	glutathione s tranferase tag:n-n|flag tag:n-n	-	-	psi-mi:"MI:0866"(tag visualisation)	psi-mi:"MI:0866"(tag visualisation)
intenz:2.3.1.225	uniprotkb:P01120	intact:EBI-12556324	intact:EBI-14838|uniprotkb:Q45U01|uniprotkb:D6W181|ensemblfungi:YNL098C	psi-mi:erf2-shr5-1(display_short)|psi-mi:2.3.1.225(display_long)|intact:Palmitoyltransferase ERF2/SHR5 complex(complex recommended name)|intact:"ERF2:SHR5"(complex systematic name)|intact:Erf2/Erf4 PAT complex(complex synonym)|intact:Palmitoyltransferase ERF2-SHR5 complex(complex synonym)|intact:Erf2-Erf4 PAT complex(complex synonym)	psi-mi:ras2_yeast(display_long)|uniprotkb:RAS2(gene name)|psi-mi:RAS2(display_short)|uniprotkb:ASC1(gene name synonym)|uniprotkb:CTN5(gene name synonym)|uniprotkb:GLC5(gene name synonym)|uniprotkb:YNL098C(locus name)|uniprotkb:N2198(orf name)	psi-mi:"MI:1004"(palmitoylase assay)	Lobo et al. (2002)	pubmed:12193598|imex:IM-25640	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	psi-mi:"MI:0216"(palmitoylation reaction)	psi-mi:"MI:0469"(IntAct)	intact:EBI-13637798|imex:IM-25640-5	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0501"(enzyme)	psi-mi:"MI:0502"(enzyme target)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:1302"(stable complex)	psi-mi:"MI:0326"(protein)	pubmed:20851885(see-also)|pubmed:10490616(see-also)|pubmed:12193598(see-also)|intact:EBI-12556304(exp-evidence)|go:"GO:0018230"(peptidyl-L-cysteine S-palmitoylation)|go:"GO:0031211"(endoplasmic reticulum palmitoyltransferase complex)|go:"GO:0030176"(integral component of endoplasmic reticulum membrane)|go:"GO:0019706"(protein-cysteine S-palmitoyltransferase activity)|evidence ontology:"ECO:0000353"|complex portal:CPX-813(complex-primary)	refseq:NP_014301.1|sgd:S000005042|go:"GO:0032880"(regulation of protein localization)|go:"GO:0045762"(positive regulation of adenylate cyclase activity)|go:"GO:0071944"(cell periphery)|go:"GO:0097271"(protein localization to bud neck)|go:"GO:2000222"(positive regulation of pseudohyphal growth)|interpro:IPR001806(Ras GTPase)|interpro:IPR005225(Small GTP-binding protein)|interpro:IPR020849|interpro:IPR027417|reactome:R-SCE-9696273|go:"GO:0010603"(regulation of cytoplasmic mRNA processing body assembly)|go:"GO:0016236"(macroautophagy)|go:"GO:0019003"(GDP binding)|go:"GO:0030437"(ascospore formation)|go:"GO:0032258"(cytoplasm to vacuole transport by the Cvt pathway)|ensemblfungi:YNL098C(gene)|ensemblfungi:YNL098C_mRNA(transcript)|go:"GO:0000411"(positive regulation of transcription by galactose)|go:"GO:0003924"(GTPase activity)|go:"GO:0003925"(G protein activity)|go:"GO:0005525"(GTP binding)|go:"GO:0005634"(nucleus)|go:"GO:0005739"(mitochondrion)|go:"GO:0005789"(endoplasmic reticulum membrane)|go:"GO:0005886"(plasma membrane)|go:"GO:0007190"(activation of adenylate cyclase activity)|go:"GO:0007265"(Ras protein signal transduction)|dip:DIP-2263N|mint:P01120	go:"GO:0019706"(protein-cysteine S-palmitoyltransferase activity)	curated-complex:Palmitoyltransferase complex that catalyzes the palmitoylation of proteins through a two step reaction - the autopalmitoylation of the enzyme to create a palmitoyl-ERF2 intermediate followed by the transfer of the palmitoyl moiety to the RAS substrate. Palmitoyl-CoA serves as the palmitate donor.|complex-properties:ERF2 appears to have a direct role in palmitate transfer based on its ability to form an acyl-intermediate.	crc64:6CAAAC531EEAE92F	figure legend:Table 1|kinetics:PAT activity 2720 pmol palmitate transferred/min/μmol substrate|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2016/10/06	2017/01/26	-	rogid:MJS2Zuw6xxrxaLE6r3omwm+2vm4559292	-	false	glutathione s tranferase tag:n-n|flag tag:n-n	glutathione s tranferase tag:?-?|sufficient binding region:294-322|mutation disrupting interaction rate:318-318	-	-	psi-mi:"MI:0866"(tag visualisation)	psi-mi:"MI:0866"(tag visualisation)
chebi:"CHEBI:15525"	intenz:2.3.1.225	intact:EBI-2008688	intact:EBI-12556324	psi-mi:palmitoyl-coa(display_short)|psi-mi:"CHEBI:15525"(display_long)	psi-mi:erf2-shr5-1(display_short)|psi-mi:2.3.1.225(display_long)|intact:Palmitoyltransferase ERF2/SHR5 complex(complex recommended name)|intact:"ERF2:SHR5"(complex systematic name)|intact:Erf2/Erf4 PAT complex(complex synonym)|intact:Palmitoyltransferase ERF2-SHR5 complex(complex synonym)|intact:Erf2-Erf4 PAT complex(complex synonym)	psi-mi:"MI:1004"(palmitoylase assay)	Lobo et al. (2002)	pubmed:12193598|imex:IM-25640	taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)")	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	psi-mi:"MI:1127"(putative self interaction)	psi-mi:"MI:0469"(IntAct)	intact:EBI-13637845|imex:IM-25640-6	-	-	psi-mi:"MI:0502"(enzyme target)	psi-mi:"MI:0898"(putative self)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0328"(small molecule)	psi-mi:"MI:1302"(stable complex)	-	pubmed:20851885(see-also)|pubmed:10490616(see-also)|pubmed:12193598(see-also)|intact:EBI-12556304(exp-evidence)|go:"GO:0018230"(peptidyl-L-cysteine S-palmitoylation)|go:"GO:0031211"(endoplasmic reticulum palmitoyltransferase complex)|go:"GO:0030176"(integral component of endoplasmic reticulum membrane)|go:"GO:0019706"(protein-cysteine S-palmitoyltransferase activity)|evidence ontology:"ECO:0000353"|complex portal:CPX-813(complex-primary)	go:"GO:0019706"(protein-cysteine S-palmitoyltransferase activity)	-	curated-complex:Palmitoyltransferase complex that catalyzes the palmitoylation of proteins through a two step reaction - the autopalmitoylation of the enzyme to create a palmitoyl-ERF2 intermediate followed by the transfer of the palmitoyl moiety to the RAS substrate. Palmitoyl-CoA serves as the palmitate donor.|complex-properties:ERF2 appears to have a direct role in palmitate transfer based on its ability to form an acyl-intermediate.	figure legend:Fig. 4|comment:Formation of a [3H]palmitoylated Erf2p acyl-enzyme intermediate. Erf2 Cys189Ser and His201Ala mutants abolished the catalytic activity of the enzyme complex|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2016/10/06	2017/01/26	-	-	-	false	3h radiolabel:?-?	glutathione s tranferase tag:n-n|flag tag:n-n	-	-	psi-mi:"MI:0866"(tag visualisation)	psi-mi:"MI:0866"(tag visualisation)
chebi:"CHEBI:15525"	intenz:2.3.1.225	intact:EBI-2008688	intact:EBI-12556324	psi-mi:palmitoyl-coa(display_short)|psi-mi:"CHEBI:15525"(display_long)	psi-mi:erf2-shr5-1(display_short)|psi-mi:2.3.1.225(display_long)|intact:Palmitoyltransferase ERF2/SHR5 complex(complex recommended name)|intact:"ERF2:SHR5"(complex systematic name)|intact:Erf2/Erf4 PAT complex(complex synonym)|intact:Palmitoyltransferase ERF2-SHR5 complex(complex synonym)|intact:Erf2-Erf4 PAT complex(complex synonym)	psi-mi:"MI:1004"(palmitoylase assay)	Lobo et al. (2002)	pubmed:12193598|imex:IM-25640	taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)")	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	psi-mi:"MI:0216"(palmitoylation reaction)	psi-mi:"MI:0469"(IntAct)	intact:EBI-13637886|imex:IM-25640-7	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0941"(competitor)	psi-mi:"MI:0501"(enzyme)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0328"(small molecule)	psi-mi:"MI:1302"(stable complex)	-	pubmed:20851885(see-also)|pubmed:10490616(see-also)|pubmed:12193598(see-also)|intact:EBI-12556304(exp-evidence)|go:"GO:0018230"(peptidyl-L-cysteine S-palmitoylation)|go:"GO:0031211"(endoplasmic reticulum palmitoyltransferase complex)|go:"GO:0030176"(integral component of endoplasmic reticulum membrane)|go:"GO:0019706"(protein-cysteine S-palmitoyltransferase activity)|evidence ontology:"ECO:0000353"|complex portal:CPX-813(complex-primary)	go:"GO:0019706"(protein-cysteine S-palmitoyltransferase activity)	-	curated-complex:Palmitoyltransferase complex that catalyzes the palmitoylation of proteins through a two step reaction - the autopalmitoylation of the enzyme to create a palmitoyl-ERF2 intermediate followed by the transfer of the palmitoyl moiety to the RAS substrate. Palmitoyl-CoA serves as the palmitate donor.|complex-properties:ERF2 appears to have a direct role in palmitate transfer based on its ability to form an acyl-intermediate.	figure legend:Fig 5|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2016/10/06	2017/01/26	-	-	-	false	-	glutathione s tranferase tag:n-n|flag tag:n-n	-	-	psi-mi:"MI:0866"(tag visualisation)	psi-mi:"MI:0866"(tag visualisation)
chebi:"CHEBI:15525"	intenz:2.3.1.225	intact:EBI-2008688	intact:EBI-12556324	psi-mi:palmitoyl-coa(display_short)|psi-mi:"CHEBI:15525"(display_long)	psi-mi:erf2-shr5-1(display_short)|psi-mi:2.3.1.225(display_long)|intact:Palmitoyltransferase ERF2/SHR5 complex(complex recommended name)|intact:"ERF2:SHR5"(complex systematic name)|intact:Erf2/Erf4 PAT complex(complex synonym)|intact:Palmitoyltransferase ERF2-SHR5 complex(complex synonym)|intact:Erf2-Erf4 PAT complex(complex synonym)	psi-mi:"MI:1004"(palmitoylase assay)	Lobo et al. (2002)	pubmed:12193598|imex:IM-25640	taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)")	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	psi-mi:"MI:0216"(palmitoylation reaction)	psi-mi:"MI:0469"(IntAct)	intact:EBI-13637886|imex:IM-25640-7	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0502"(enzyme target)	psi-mi:"MI:0501"(enzyme)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0328"(small molecule)	psi-mi:"MI:1302"(stable complex)	-	pubmed:20851885(see-also)|pubmed:10490616(see-also)|pubmed:12193598(see-also)|intact:EBI-12556304(exp-evidence)|go:"GO:0018230"(peptidyl-L-cysteine S-palmitoylation)|go:"GO:0031211"(endoplasmic reticulum palmitoyltransferase complex)|go:"GO:0030176"(integral component of endoplasmic reticulum membrane)|go:"GO:0019706"(protein-cysteine S-palmitoyltransferase activity)|evidence ontology:"ECO:0000353"|complex portal:CPX-813(complex-primary)	go:"GO:0019706"(protein-cysteine S-palmitoyltransferase activity)	-	curated-complex:Palmitoyltransferase complex that catalyzes the palmitoylation of proteins through a two step reaction - the autopalmitoylation of the enzyme to create a palmitoyl-ERF2 intermediate followed by the transfer of the palmitoyl moiety to the RAS substrate. Palmitoyl-CoA serves as the palmitate donor.|complex-properties:ERF2 appears to have a direct role in palmitate transfer based on its ability to form an acyl-intermediate.	figure legend:Fig 5|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2016/10/06	2017/01/26	-	-	-	false	3h radiolabel:?-?	glutathione s tranferase tag:n-n|flag tag:n-n	-	-	psi-mi:"MI:0866"(tag visualisation)	psi-mi:"MI:0866"(tag visualisation)
intenz:2.3.1.225	uniprotkb:P01120	intact:EBI-12556324	intact:EBI-14838|uniprotkb:Q45U01|uniprotkb:D6W181|ensemblfungi:YNL098C	psi-mi:erf2-shr5-1(display_short)|psi-mi:2.3.1.225(display_long)|intact:Palmitoyltransferase ERF2/SHR5 complex(complex recommended name)|intact:"ERF2:SHR5"(complex systematic name)|intact:Erf2/Erf4 PAT complex(complex synonym)|intact:Palmitoyltransferase ERF2-SHR5 complex(complex synonym)|intact:Erf2-Erf4 PAT complex(complex synonym)	psi-mi:ras2_yeast(display_long)|uniprotkb:RAS2(gene name)|psi-mi:RAS2(display_short)|uniprotkb:ASC1(gene name synonym)|uniprotkb:CTN5(gene name synonym)|uniprotkb:GLC5(gene name synonym)|uniprotkb:YNL098C(locus name)|uniprotkb:N2198(orf name)	psi-mi:"MI:1004"(palmitoylase assay)	Lobo et al. (2002)	pubmed:12193598|imex:IM-25640	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	psi-mi:"MI:0216"(palmitoylation reaction)	psi-mi:"MI:0469"(IntAct)	intact:EBI-13637886|imex:IM-25640-7	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0501"(enzyme)	psi-mi:"MI:0502"(enzyme target)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:1302"(stable complex)	psi-mi:"MI:0326"(protein)	pubmed:20851885(see-also)|pubmed:10490616(see-also)|pubmed:12193598(see-also)|intact:EBI-12556304(exp-evidence)|go:"GO:0018230"(peptidyl-L-cysteine S-palmitoylation)|go:"GO:0031211"(endoplasmic reticulum palmitoyltransferase complex)|go:"GO:0030176"(integral component of endoplasmic reticulum membrane)|go:"GO:0019706"(protein-cysteine S-palmitoyltransferase activity)|evidence ontology:"ECO:0000353"|complex portal:CPX-813(complex-primary)	refseq:NP_014301.1|sgd:S000005042|go:"GO:0032880"(regulation of protein localization)|go:"GO:0045762"(positive regulation of adenylate cyclase activity)|go:"GO:0071944"(cell periphery)|go:"GO:0097271"(protein localization to bud neck)|go:"GO:2000222"(positive regulation of pseudohyphal growth)|interpro:IPR001806(Ras GTPase)|interpro:IPR005225(Small GTP-binding protein)|interpro:IPR020849|interpro:IPR027417|reactome:R-SCE-9696273|go:"GO:0010603"(regulation of cytoplasmic mRNA processing body assembly)|go:"GO:0016236"(macroautophagy)|go:"GO:0019003"(GDP binding)|go:"GO:0030437"(ascospore formation)|go:"GO:0032258"(cytoplasm to vacuole transport by the Cvt pathway)|ensemblfungi:YNL098C(gene)|ensemblfungi:YNL098C_mRNA(transcript)|go:"GO:0000411"(positive regulation of transcription by galactose)|go:"GO:0003924"(GTPase activity)|go:"GO:0003925"(G protein activity)|go:"GO:0005525"(GTP binding)|go:"GO:0005634"(nucleus)|go:"GO:0005739"(mitochondrion)|go:"GO:0005789"(endoplasmic reticulum membrane)|go:"GO:0005886"(plasma membrane)|go:"GO:0007190"(activation of adenylate cyclase activity)|go:"GO:0007265"(Ras protein signal transduction)|dip:DIP-2263N|mint:P01120	go:"GO:0019706"(protein-cysteine S-palmitoyltransferase activity)	curated-complex:Palmitoyltransferase complex that catalyzes the palmitoylation of proteins through a two step reaction - the autopalmitoylation of the enzyme to create a palmitoyl-ERF2 intermediate followed by the transfer of the palmitoyl moiety to the RAS substrate. Palmitoyl-CoA serves as the palmitate donor.|complex-properties:ERF2 appears to have a direct role in palmitate transfer based on its ability to form an acyl-intermediate.	crc64:6CAAAC531EEAE92F	figure legend:Fig 5|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2016/10/06	2017/01/26	-	rogid:MJS2Zuw6xxrxaLE6r3omwm+2vm4559292	-	false	glutathione s tranferase tag:n-n|flag tag:n-n	glutathione s tranferase tag:?-?|sufficient binding region:294-322|mutation disrupting interaction rate:318-318	-	-	psi-mi:"MI:0866"(tag visualisation)	psi-mi:"MI:0866"(tag visualisation)
chebi:"CHEBI:53152"	intenz:2.3.1.225	intact:EBI-13638012	intact:EBI-12556324	psi-mi:palmitoleoyl-coa(display_short)|psi-mi:"CHEBI:53152"(display_long)|intact:"(Z)-9-hexadecenoyl-CoA"(synonym)|intact:cis-9-hexadecenoyl-CoA(synonym)|intact:cis-9-hexadecenoyl-coenzyme A(synonym)|intact:palmitoleoyl-coenzyme A(synonym)|intact:palmitoleyl-CoA(synonym)|intact:palmitoleyl-coenzyme A(synonym)|intact:"3'-phosphoadenosine 5'-{3-[(3R)-3-hydroxy-2,2-dimethyl-4-({3-[(2-{[(9Z)-hexadec-9-enoyl]sulfanyl}ethyl)amino]-3-oxopropyl}amino)-4-oxobutyl] dihydrogen diphosphate}"(iupac name)	psi-mi:erf2-shr5-1(display_short)|psi-mi:2.3.1.225(display_long)|intact:Palmitoyltransferase ERF2/SHR5 complex(complex recommended name)|intact:"ERF2:SHR5"(complex systematic name)|intact:Erf2/Erf4 PAT complex(complex synonym)|intact:Palmitoyltransferase ERF2-SHR5 complex(complex synonym)|intact:Erf2-Erf4 PAT complex(complex synonym)	psi-mi:"MI:1004"(palmitoylase assay)	Lobo et al. (2002)	pubmed:12193598|imex:IM-25640	taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)")	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	psi-mi:"MI:0216"(palmitoylation reaction)	psi-mi:"MI:0469"(IntAct)	intact:EBI-13638021|imex:IM-25640-8	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0941"(competitor)	psi-mi:"MI:0501"(enzyme)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0328"(small molecule)	psi-mi:"MI:1302"(stable complex)	-	pubmed:20851885(see-also)|pubmed:10490616(see-also)|pubmed:12193598(see-also)|intact:EBI-12556304(exp-evidence)|go:"GO:0018230"(peptidyl-L-cysteine S-palmitoylation)|go:"GO:0031211"(endoplasmic reticulum palmitoyltransferase complex)|go:"GO:0030176"(integral component of endoplasmic reticulum membrane)|go:"GO:0019706"(protein-cysteine S-palmitoyltransferase activity)|evidence ontology:"ECO:0000353"|complex portal:CPX-813(complex-primary)	go:"GO:0019706"(protein-cysteine S-palmitoyltransferase activity)	-	curated-complex:Palmitoyltransferase complex that catalyzes the palmitoylation of proteins through a two step reaction - the autopalmitoylation of the enzyme to create a palmitoyl-ERF2 intermediate followed by the transfer of the palmitoyl moiety to the RAS substrate. Palmitoyl-CoA serves as the palmitate donor.|complex-properties:ERF2 appears to have a direct role in palmitate transfer based on its ability to form an acyl-intermediate.	figure legend:Fig 5|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2016/10/06	2017/01/26	-	-	-	false	-	glutathione s tranferase tag:n-n|flag tag:n-n	-	-	psi-mi:"MI:0866"(tag visualisation)	psi-mi:"MI:0866"(tag visualisation)
chebi:"CHEBI:15525"	intenz:2.3.1.225	intact:EBI-2008688	intact:EBI-12556324	psi-mi:palmitoyl-coa(display_short)|psi-mi:"CHEBI:15525"(display_long)	psi-mi:erf2-shr5-1(display_short)|psi-mi:2.3.1.225(display_long)|intact:Palmitoyltransferase ERF2/SHR5 complex(complex recommended name)|intact:"ERF2:SHR5"(complex systematic name)|intact:Erf2/Erf4 PAT complex(complex synonym)|intact:Palmitoyltransferase ERF2-SHR5 complex(complex synonym)|intact:Erf2-Erf4 PAT complex(complex synonym)	psi-mi:"MI:1004"(palmitoylase assay)	Lobo et al. (2002)	pubmed:12193598|imex:IM-25640	taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)")	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	psi-mi:"MI:0216"(palmitoylation reaction)	psi-mi:"MI:0469"(IntAct)	intact:EBI-13638021|imex:IM-25640-8	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0502"(enzyme target)	psi-mi:"MI:0501"(enzyme)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0328"(small molecule)	psi-mi:"MI:1302"(stable complex)	-	pubmed:20851885(see-also)|pubmed:10490616(see-also)|pubmed:12193598(see-also)|intact:EBI-12556304(exp-evidence)|go:"GO:0018230"(peptidyl-L-cysteine S-palmitoylation)|go:"GO:0031211"(endoplasmic reticulum palmitoyltransferase complex)|go:"GO:0030176"(integral component of endoplasmic reticulum membrane)|go:"GO:0019706"(protein-cysteine S-palmitoyltransferase activity)|evidence ontology:"ECO:0000353"|complex portal:CPX-813(complex-primary)	go:"GO:0019706"(protein-cysteine S-palmitoyltransferase activity)	-	curated-complex:Palmitoyltransferase complex that catalyzes the palmitoylation of proteins through a two step reaction - the autopalmitoylation of the enzyme to create a palmitoyl-ERF2 intermediate followed by the transfer of the palmitoyl moiety to the RAS substrate. Palmitoyl-CoA serves as the palmitate donor.|complex-properties:ERF2 appears to have a direct role in palmitate transfer based on its ability to form an acyl-intermediate.	figure legend:Fig 5|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2016/10/06	2017/01/26	-	-	-	false	3h radiolabel:?-?	glutathione s tranferase tag:n-n|flag tag:n-n	-	-	psi-mi:"MI:0866"(tag visualisation)	psi-mi:"MI:0866"(tag visualisation)
intenz:2.3.1.225	uniprotkb:P01120	intact:EBI-12556324	intact:EBI-14838|uniprotkb:Q45U01|uniprotkb:D6W181|ensemblfungi:YNL098C	psi-mi:erf2-shr5-1(display_short)|psi-mi:2.3.1.225(display_long)|intact:Palmitoyltransferase ERF2/SHR5 complex(complex recommended name)|intact:"ERF2:SHR5"(complex systematic name)|intact:Erf2/Erf4 PAT complex(complex synonym)|intact:Palmitoyltransferase ERF2-SHR5 complex(complex synonym)|intact:Erf2-Erf4 PAT complex(complex synonym)	psi-mi:ras2_yeast(display_long)|uniprotkb:RAS2(gene name)|psi-mi:RAS2(display_short)|uniprotkb:ASC1(gene name synonym)|uniprotkb:CTN5(gene name synonym)|uniprotkb:GLC5(gene name synonym)|uniprotkb:YNL098C(locus name)|uniprotkb:N2198(orf name)	psi-mi:"MI:1004"(palmitoylase assay)	Lobo et al. (2002)	pubmed:12193598|imex:IM-25640	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	psi-mi:"MI:0216"(palmitoylation reaction)	psi-mi:"MI:0469"(IntAct)	intact:EBI-13638021|imex:IM-25640-8	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0501"(enzyme)	psi-mi:"MI:0502"(enzyme target)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:1302"(stable complex)	psi-mi:"MI:0326"(protein)	pubmed:20851885(see-also)|pubmed:10490616(see-also)|pubmed:12193598(see-also)|intact:EBI-12556304(exp-evidence)|go:"GO:0018230"(peptidyl-L-cysteine S-palmitoylation)|go:"GO:0031211"(endoplasmic reticulum palmitoyltransferase complex)|go:"GO:0030176"(integral component of endoplasmic reticulum membrane)|go:"GO:0019706"(protein-cysteine S-palmitoyltransferase activity)|evidence ontology:"ECO:0000353"|complex portal:CPX-813(complex-primary)	refseq:NP_014301.1|sgd:S000005042|go:"GO:0032880"(regulation of protein localization)|go:"GO:0045762"(positive regulation of adenylate cyclase activity)|go:"GO:0071944"(cell periphery)|go:"GO:0097271"(protein localization to bud neck)|go:"GO:2000222"(positive regulation of pseudohyphal growth)|interpro:IPR001806(Ras GTPase)|interpro:IPR005225(Small GTP-binding protein)|interpro:IPR020849|interpro:IPR027417|reactome:R-SCE-9696273|go:"GO:0010603"(regulation of cytoplasmic mRNA processing body assembly)|go:"GO:0016236"(macroautophagy)|go:"GO:0019003"(GDP binding)|go:"GO:0030437"(ascospore formation)|go:"GO:0032258"(cytoplasm to vacuole transport by the Cvt pathway)|ensemblfungi:YNL098C(gene)|ensemblfungi:YNL098C_mRNA(transcript)|go:"GO:0000411"(positive regulation of transcription by galactose)|go:"GO:0003924"(GTPase activity)|go:"GO:0003925"(G protein activity)|go:"GO:0005525"(GTP binding)|go:"GO:0005634"(nucleus)|go:"GO:0005739"(mitochondrion)|go:"GO:0005789"(endoplasmic reticulum membrane)|go:"GO:0005886"(plasma membrane)|go:"GO:0007190"(activation of adenylate cyclase activity)|go:"GO:0007265"(Ras protein signal transduction)|dip:DIP-2263N|mint:P01120	go:"GO:0019706"(protein-cysteine S-palmitoyltransferase activity)	curated-complex:Palmitoyltransferase complex that catalyzes the palmitoylation of proteins through a two step reaction - the autopalmitoylation of the enzyme to create a palmitoyl-ERF2 intermediate followed by the transfer of the palmitoyl moiety to the RAS substrate. Palmitoyl-CoA serves as the palmitate donor.|complex-properties:ERF2 appears to have a direct role in palmitate transfer based on its ability to form an acyl-intermediate.	crc64:6CAAAC531EEAE92F	figure legend:Fig 5|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2016/10/06	2017/01/26	-	rogid:MJS2Zuw6xxrxaLE6r3omwm+2vm4559292	-	false	glutathione s tranferase tag:n-n|flag tag:n-n	glutathione s tranferase tag:?-?|sufficient binding region:294-322|mutation disrupting interaction rate:318-318	-	-	psi-mi:"MI:0866"(tag visualisation)	psi-mi:"MI:0866"(tag visualisation)
chebi:"CHEBI:15525"	intenz:2.3.1.225	intact:EBI-2008688	intact:EBI-12556324	psi-mi:palmitoyl-coa(display_short)|psi-mi:"CHEBI:15525"(display_long)	psi-mi:erf2-shr5-1(display_short)|psi-mi:2.3.1.225(display_long)|intact:Palmitoyltransferase ERF2/SHR5 complex(complex recommended name)|intact:"ERF2:SHR5"(complex systematic name)|intact:Erf2/Erf4 PAT complex(complex synonym)|intact:Palmitoyltransferase ERF2-SHR5 complex(complex synonym)|intact:Erf2-Erf4 PAT complex(complex synonym)	psi-mi:"MI:1004"(palmitoylase assay)	Lobo et al. (2002)	pubmed:12193598|imex:IM-25640	taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)")	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	psi-mi:"MI:0216"(palmitoylation reaction)	psi-mi:"MI:0469"(IntAct)	intact:EBI-13638057|imex:IM-25640-9	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0502"(enzyme target)	psi-mi:"MI:0501"(enzyme)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0328"(small molecule)	psi-mi:"MI:1302"(stable complex)	-	pubmed:20851885(see-also)|pubmed:10490616(see-also)|pubmed:12193598(see-also)|intact:EBI-12556304(exp-evidence)|go:"GO:0018230"(peptidyl-L-cysteine S-palmitoylation)|go:"GO:0031211"(endoplasmic reticulum palmitoyltransferase complex)|go:"GO:0030176"(integral component of endoplasmic reticulum membrane)|go:"GO:0019706"(protein-cysteine S-palmitoyltransferase activity)|evidence ontology:"ECO:0000353"|complex portal:CPX-813(complex-primary)	go:"GO:0019706"(protein-cysteine S-palmitoyltransferase activity)	-	curated-complex:Palmitoyltransferase complex that catalyzes the palmitoylation of proteins through a two step reaction - the autopalmitoylation of the enzyme to create a palmitoyl-ERF2 intermediate followed by the transfer of the palmitoyl moiety to the RAS substrate. Palmitoyl-CoA serves as the palmitate donor.|complex-properties:ERF2 appears to have a direct role in palmitate transfer based on its ability to form an acyl-intermediate.	figure legend:Fig 5|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2016/10/06	2017/01/26	-	-	-	false	3h radiolabel:?-?	glutathione s tranferase tag:n-n|flag tag:n-n	-	-	psi-mi:"MI:0866"(tag visualisation)	psi-mi:"MI:0866"(tag visualisation)
intenz:2.3.1.225	uniprotkb:P01120	intact:EBI-12556324	intact:EBI-14838|uniprotkb:Q45U01|uniprotkb:D6W181|ensemblfungi:YNL098C	psi-mi:erf2-shr5-1(display_short)|psi-mi:2.3.1.225(display_long)|intact:Palmitoyltransferase ERF2/SHR5 complex(complex recommended name)|intact:"ERF2:SHR5"(complex systematic name)|intact:Erf2/Erf4 PAT complex(complex synonym)|intact:Palmitoyltransferase ERF2-SHR5 complex(complex synonym)|intact:Erf2-Erf4 PAT complex(complex synonym)	psi-mi:ras2_yeast(display_long)|uniprotkb:RAS2(gene name)|psi-mi:RAS2(display_short)|uniprotkb:ASC1(gene name synonym)|uniprotkb:CTN5(gene name synonym)|uniprotkb:GLC5(gene name synonym)|uniprotkb:YNL098C(locus name)|uniprotkb:N2198(orf name)	psi-mi:"MI:1004"(palmitoylase assay)	Lobo et al. (2002)	pubmed:12193598|imex:IM-25640	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	psi-mi:"MI:0216"(palmitoylation reaction)	psi-mi:"MI:0469"(IntAct)	intact:EBI-13638057|imex:IM-25640-9	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0501"(enzyme)	psi-mi:"MI:0502"(enzyme target)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:1302"(stable complex)	psi-mi:"MI:0326"(protein)	pubmed:20851885(see-also)|pubmed:10490616(see-also)|pubmed:12193598(see-also)|intact:EBI-12556304(exp-evidence)|go:"GO:0018230"(peptidyl-L-cysteine S-palmitoylation)|go:"GO:0031211"(endoplasmic reticulum palmitoyltransferase complex)|go:"GO:0030176"(integral component of endoplasmic reticulum membrane)|go:"GO:0019706"(protein-cysteine S-palmitoyltransferase activity)|evidence ontology:"ECO:0000353"|complex portal:CPX-813(complex-primary)	refseq:NP_014301.1|sgd:S000005042|go:"GO:0032880"(regulation of protein localization)|go:"GO:0045762"(positive regulation of adenylate cyclase activity)|go:"GO:0071944"(cell periphery)|go:"GO:0097271"(protein localization to bud neck)|go:"GO:2000222"(positive regulation of pseudohyphal growth)|interpro:IPR001806(Ras GTPase)|interpro:IPR005225(Small GTP-binding protein)|interpro:IPR020849|interpro:IPR027417|reactome:R-SCE-9696273|go:"GO:0010603"(regulation of cytoplasmic mRNA processing body assembly)|go:"GO:0016236"(macroautophagy)|go:"GO:0019003"(GDP binding)|go:"GO:0030437"(ascospore formation)|go:"GO:0032258"(cytoplasm to vacuole transport by the Cvt pathway)|ensemblfungi:YNL098C(gene)|ensemblfungi:YNL098C_mRNA(transcript)|go:"GO:0000411"(positive regulation of transcription by galactose)|go:"GO:0003924"(GTPase activity)|go:"GO:0003925"(G protein activity)|go:"GO:0005525"(GTP binding)|go:"GO:0005634"(nucleus)|go:"GO:0005739"(mitochondrion)|go:"GO:0005789"(endoplasmic reticulum membrane)|go:"GO:0005886"(plasma membrane)|go:"GO:0007190"(activation of adenylate cyclase activity)|go:"GO:0007265"(Ras protein signal transduction)|dip:DIP-2263N|mint:P01120	go:"GO:0019706"(protein-cysteine S-palmitoyltransferase activity)	curated-complex:Palmitoyltransferase complex that catalyzes the palmitoylation of proteins through a two step reaction - the autopalmitoylation of the enzyme to create a palmitoyl-ERF2 intermediate followed by the transfer of the palmitoyl moiety to the RAS substrate. Palmitoyl-CoA serves as the palmitate donor.|complex-properties:ERF2 appears to have a direct role in palmitate transfer based on its ability to form an acyl-intermediate.	crc64:6CAAAC531EEAE92F	figure legend:Fig 5|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2016/10/06	2017/01/26	-	rogid:MJS2Zuw6xxrxaLE6r3omwm+2vm4559292	-	false	glutathione s tranferase tag:n-n|flag tag:n-n	glutathione s tranferase tag:?-?|sufficient binding region:294-322|mutation disrupting interaction rate:318-318	-	-	psi-mi:"MI:0866"(tag visualisation)	psi-mi:"MI:0866"(tag visualisation)
chebi:"CHEBI:15541"	intenz:2.3.1.225	intact:EBI-13638041|chebi:"CHEBI:9256"|chebi:"CHEBI:15107"|chebi:"CHEBI:26754"	intact:EBI-12556324	psi-mi:stearoyl-coa(display_short)|psi-mi:"CHEBI:15541"(display_long)|intact:"C18:0-CoA"(synonym)|intact:"C18:0-coenzyme A"(synonym)|intact:octadecanoyl-CoA(synonym)|intact:octadecanoyl-coenzyme A(synonym)|intact:S-stearoyl-CoA(synonym)|intact:S-stearoylcoenzyme A(synonym)|intact:Stearoyl-CoA(synonym)|intact:stearoyl-coenzyme A(synonym)|intact:Stearyl coenzyme A(synonym)|intact:Stearyl-CoA(synonym)|intact:"3'-phosphoadenosine 5'-(3-{(3R)-3-hydroxy-2,2-dimethyl-4-[(3-{[2-(octadecanoylsulfanyl)ethyl]amino}-3-oxopropyl)amino]-4-oxobutyl} dihydrogen diphosphate)"(iupac name)	psi-mi:erf2-shr5-1(display_short)|psi-mi:2.3.1.225(display_long)|intact:Palmitoyltransferase ERF2/SHR5 complex(complex recommended name)|intact:"ERF2:SHR5"(complex systematic name)|intact:Erf2/Erf4 PAT complex(complex synonym)|intact:Palmitoyltransferase ERF2-SHR5 complex(complex synonym)|intact:Erf2-Erf4 PAT complex(complex synonym)	psi-mi:"MI:1004"(palmitoylase assay)	Lobo et al. (2002)	pubmed:12193598|imex:IM-25640	taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)")	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	psi-mi:"MI:0216"(palmitoylation reaction)	psi-mi:"MI:0469"(IntAct)	intact:EBI-13638057|imex:IM-25640-9	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0941"(competitor)	psi-mi:"MI:0501"(enzyme)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0328"(small molecule)	psi-mi:"MI:1302"(stable complex)	-	pubmed:20851885(see-also)|pubmed:10490616(see-also)|pubmed:12193598(see-also)|intact:EBI-12556304(exp-evidence)|go:"GO:0018230"(peptidyl-L-cysteine S-palmitoylation)|go:"GO:0031211"(endoplasmic reticulum palmitoyltransferase complex)|go:"GO:0030176"(integral component of endoplasmic reticulum membrane)|go:"GO:0019706"(protein-cysteine S-palmitoyltransferase activity)|evidence ontology:"ECO:0000353"|complex portal:CPX-813(complex-primary)	go:"GO:0019706"(protein-cysteine S-palmitoyltransferase activity)	-	curated-complex:Palmitoyltransferase complex that catalyzes the palmitoylation of proteins through a two step reaction - the autopalmitoylation of the enzyme to create a palmitoyl-ERF2 intermediate followed by the transfer of the palmitoyl moiety to the RAS substrate. Palmitoyl-CoA serves as the palmitate donor.|complex-properties:ERF2 appears to have a direct role in palmitate transfer based on its ability to form an acyl-intermediate.	figure legend:Fig 5|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2016/10/06	2017/01/26	-	-	-	false	-	glutathione s tranferase tag:n-n|flag tag:n-n	-	-	psi-mi:"MI:0866"(tag visualisation)	psi-mi:"MI:0866"(tag visualisation)
chebi:"CHEBI:15534"	intenz:2.3.1.225	intact:EBI-13638077|chebi:"CHEBI:7743"|chebi:"CHEBI:14685"|chebi:"CHEBI:25668"	intact:EBI-12556324	psi-mi:oleoyl-coa(display_short)|psi-mi:"CHEBI:15534"(display_long)|intact:"(9Z)-octadec-9-enoyl-CoA"(synonym)|intact:"(9Z)-octadec-9-enoyl-coenzyme A"(synonym)|intact:"(9Z)-Octadecenoyl-CoA"(synonym)|intact:cis-9-octadecenoyl-CoA(synonym)|intact:cis-9-octadecenoyl-coenzyme A(synonym)|intact:"cis-Delta(9)-octadecenoyl-CoA"(synonym)|intact:"cis-Delta(9)-octadecenoyl-coenzyme A"(synonym)|intact:Oleoyl-CoA(synonym)|intact:Oleoyl-coenzyme A(synonym)|intact:"S-[(9Z)-octadec-9-enoyl]-CoA"(synonym)|intact:"S-[(9Z)-octadec-9-enoyl]-coenzyme A"(synonym)|intact:S-oleoyl-CoA(synonym)|intact:S-Oleoylcoenzyme A(synonym)|intact:"3'-phosphoadenosine 5'-{3-[(3R)-3-hydroxy-2,2-dimethyl-4-({3-[(2-{[(9Z)-octadec-9-enoyl]sulfanyl}ethyl)amino]-3-oxopropyl}amino)-4-oxobutyl] dihydrogen diphosphate}"(iupac name)	psi-mi:erf2-shr5-1(display_short)|psi-mi:2.3.1.225(display_long)|intact:Palmitoyltransferase ERF2/SHR5 complex(complex recommended name)|intact:"ERF2:SHR5"(complex systematic name)|intact:Erf2/Erf4 PAT complex(complex synonym)|intact:Palmitoyltransferase ERF2-SHR5 complex(complex synonym)|intact:Erf2-Erf4 PAT complex(complex synonym)	psi-mi:"MI:1004"(palmitoylase assay)	Lobo et al. (2002)	pubmed:12193598|imex:IM-25640	taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)")	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	psi-mi:"MI:0216"(palmitoylation reaction)	psi-mi:"MI:0469"(IntAct)	intact:EBI-13638096|imex:IM-25640-10	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0941"(competitor)	psi-mi:"MI:0501"(enzyme)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0328"(small molecule)	psi-mi:"MI:1302"(stable complex)	-	pubmed:20851885(see-also)|pubmed:10490616(see-also)|pubmed:12193598(see-also)|intact:EBI-12556304(exp-evidence)|go:"GO:0018230"(peptidyl-L-cysteine S-palmitoylation)|go:"GO:0031211"(endoplasmic reticulum palmitoyltransferase complex)|go:"GO:0030176"(integral component of endoplasmic reticulum membrane)|go:"GO:0019706"(protein-cysteine S-palmitoyltransferase activity)|evidence ontology:"ECO:0000353"|complex portal:CPX-813(complex-primary)	go:"GO:0019706"(protein-cysteine S-palmitoyltransferase activity)	-	curated-complex:Palmitoyltransferase complex that catalyzes the palmitoylation of proteins through a two step reaction - the autopalmitoylation of the enzyme to create a palmitoyl-ERF2 intermediate followed by the transfer of the palmitoyl moiety to the RAS substrate. Palmitoyl-CoA serves as the palmitate donor.|complex-properties:ERF2 appears to have a direct role in palmitate transfer based on its ability to form an acyl-intermediate.	figure legend:Fig 5|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2016/10/06	2017/01/26	-	-	-	false	-	glutathione s tranferase tag:n-n|flag tag:n-n	-	-	psi-mi:"MI:0866"(tag visualisation)	psi-mi:"MI:0866"(tag visualisation)
chebi:"CHEBI:15525"	intenz:2.3.1.225	intact:EBI-2008688	intact:EBI-12556324	psi-mi:palmitoyl-coa(display_short)|psi-mi:"CHEBI:15525"(display_long)	psi-mi:erf2-shr5-1(display_short)|psi-mi:2.3.1.225(display_long)|intact:Palmitoyltransferase ERF2/SHR5 complex(complex recommended name)|intact:"ERF2:SHR5"(complex systematic name)|intact:Erf2/Erf4 PAT complex(complex synonym)|intact:Palmitoyltransferase ERF2-SHR5 complex(complex synonym)|intact:Erf2-Erf4 PAT complex(complex synonym)	psi-mi:"MI:1004"(palmitoylase assay)	Lobo et al. (2002)	pubmed:12193598|imex:IM-25640	taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)")	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	psi-mi:"MI:0216"(palmitoylation reaction)	psi-mi:"MI:0469"(IntAct)	intact:EBI-13638096|imex:IM-25640-10	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0502"(enzyme target)	psi-mi:"MI:0501"(enzyme)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0328"(small molecule)	psi-mi:"MI:1302"(stable complex)	-	pubmed:20851885(see-also)|pubmed:10490616(see-also)|pubmed:12193598(see-also)|intact:EBI-12556304(exp-evidence)|go:"GO:0018230"(peptidyl-L-cysteine S-palmitoylation)|go:"GO:0031211"(endoplasmic reticulum palmitoyltransferase complex)|go:"GO:0030176"(integral component of endoplasmic reticulum membrane)|go:"GO:0019706"(protein-cysteine S-palmitoyltransferase activity)|evidence ontology:"ECO:0000353"|complex portal:CPX-813(complex-primary)	go:"GO:0019706"(protein-cysteine S-palmitoyltransferase activity)	-	curated-complex:Palmitoyltransferase complex that catalyzes the palmitoylation of proteins through a two step reaction - the autopalmitoylation of the enzyme to create a palmitoyl-ERF2 intermediate followed by the transfer of the palmitoyl moiety to the RAS substrate. Palmitoyl-CoA serves as the palmitate donor.|complex-properties:ERF2 appears to have a direct role in palmitate transfer based on its ability to form an acyl-intermediate.	figure legend:Fig 5|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2016/10/06	2017/01/26	-	-	-	false	3h radiolabel:?-?	glutathione s tranferase tag:n-n|flag tag:n-n	-	-	psi-mi:"MI:0866"(tag visualisation)	psi-mi:"MI:0866"(tag visualisation)
intenz:2.3.1.225	uniprotkb:P01120	intact:EBI-12556324	intact:EBI-14838|uniprotkb:Q45U01|uniprotkb:D6W181|ensemblfungi:YNL098C	psi-mi:erf2-shr5-1(display_short)|psi-mi:2.3.1.225(display_long)|intact:Palmitoyltransferase ERF2/SHR5 complex(complex recommended name)|intact:"ERF2:SHR5"(complex systematic name)|intact:Erf2/Erf4 PAT complex(complex synonym)|intact:Palmitoyltransferase ERF2-SHR5 complex(complex synonym)|intact:Erf2-Erf4 PAT complex(complex synonym)	psi-mi:ras2_yeast(display_long)|uniprotkb:RAS2(gene name)|psi-mi:RAS2(display_short)|uniprotkb:ASC1(gene name synonym)|uniprotkb:CTN5(gene name synonym)|uniprotkb:GLC5(gene name synonym)|uniprotkb:YNL098C(locus name)|uniprotkb:N2198(orf name)	psi-mi:"MI:1004"(palmitoylase assay)	Lobo et al. (2002)	pubmed:12193598|imex:IM-25640	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	psi-mi:"MI:0216"(palmitoylation reaction)	psi-mi:"MI:0469"(IntAct)	intact:EBI-13638096|imex:IM-25640-10	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0501"(enzyme)	psi-mi:"MI:0502"(enzyme target)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:1302"(stable complex)	psi-mi:"MI:0326"(protein)	pubmed:20851885(see-also)|pubmed:10490616(see-also)|pubmed:12193598(see-also)|intact:EBI-12556304(exp-evidence)|go:"GO:0018230"(peptidyl-L-cysteine S-palmitoylation)|go:"GO:0031211"(endoplasmic reticulum palmitoyltransferase complex)|go:"GO:0030176"(integral component of endoplasmic reticulum membrane)|go:"GO:0019706"(protein-cysteine S-palmitoyltransferase activity)|evidence ontology:"ECO:0000353"|complex portal:CPX-813(complex-primary)	refseq:NP_014301.1|sgd:S000005042|go:"GO:0032880"(regulation of protein localization)|go:"GO:0045762"(positive regulation of adenylate cyclase activity)|go:"GO:0071944"(cell periphery)|go:"GO:0097271"(protein localization to bud neck)|go:"GO:2000222"(positive regulation of pseudohyphal growth)|interpro:IPR001806(Ras GTPase)|interpro:IPR005225(Small GTP-binding protein)|interpro:IPR020849|interpro:IPR027417|reactome:R-SCE-9696273|go:"GO:0010603"(regulation of cytoplasmic mRNA processing body assembly)|go:"GO:0016236"(macroautophagy)|go:"GO:0019003"(GDP binding)|go:"GO:0030437"(ascospore formation)|go:"GO:0032258"(cytoplasm to vacuole transport by the Cvt pathway)|ensemblfungi:YNL098C(gene)|ensemblfungi:YNL098C_mRNA(transcript)|go:"GO:0000411"(positive regulation of transcription by galactose)|go:"GO:0003924"(GTPase activity)|go:"GO:0003925"(G protein activity)|go:"GO:0005525"(GTP binding)|go:"GO:0005634"(nucleus)|go:"GO:0005739"(mitochondrion)|go:"GO:0005789"(endoplasmic reticulum membrane)|go:"GO:0005886"(plasma membrane)|go:"GO:0007190"(activation of adenylate cyclase activity)|go:"GO:0007265"(Ras protein signal transduction)|dip:DIP-2263N|mint:P01120	go:"GO:0019706"(protein-cysteine S-palmitoyltransferase activity)	curated-complex:Palmitoyltransferase complex that catalyzes the palmitoylation of proteins through a two step reaction - the autopalmitoylation of the enzyme to create a palmitoyl-ERF2 intermediate followed by the transfer of the palmitoyl moiety to the RAS substrate. Palmitoyl-CoA serves as the palmitate donor.|complex-properties:ERF2 appears to have a direct role in palmitate transfer based on its ability to form an acyl-intermediate.	crc64:6CAAAC531EEAE92F	figure legend:Fig 5|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2016/10/06	2017/01/26	-	rogid:MJS2Zuw6xxrxaLE6r3omwm+2vm4559292	-	false	glutathione s tranferase tag:n-n|flag tag:n-n	glutathione s tranferase tag:?-?|sufficient binding region:294-322|mutation disrupting interaction rate:318-318	-	-	psi-mi:"MI:0866"(tag visualisation)	psi-mi:"MI:0866"(tag visualisation)
uniprotkb:Q06551	uniprotkb:P41912	intact:EBI-37230|uniprotkb:Q6B217|uniprotkb:D6VYP4|ensemblfungi:YLR246W	intact:EBI-2087870|uniprotkb:D6W1V7|ensemblfungi:YOL110W	psi-mi:erfb_yeast(display_long)|uniprotkb:DHHC cysteine-rich domain-containing protein ERF2(gene name synonym)|uniprotkb:Ras protein acyltransferase(gene name synonym)|uniprotkb:ERF2(gene name)|psi-mi:ERF2(display_short)|uniprotkb:YLR246W(locus name)	psi-mi:erfd_yeast(display_long)|uniprotkb:YOL110W(locus name)|uniprotkb:HRC237(orf name)|uniprotkb:SHR5(gene name)|psi-mi:SHR5(display_short)|uniprotkb:ERF4(gene name synonym)|uniprotkb:Hyperactive Ras suppressor protein 5(gene name synonym)	psi-mi:"MI:0096"(pull down)	Lobo et al. (2002)	pubmed:12193598|imex:IM-25640	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-12556595|imex:IM-25640-3	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_013347.1|sgd:S000004236|mint:Q06551|dip:DIP-4783N|ensemblfungi:YLR246W(gene)|ensemblfungi:YLR246W_mRNA(transcript)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0006612"(protein targeting to membrane)|go:"GO:0016409"(palmitoyltransferase activity)|go:"GO:0018230"(peptidyl-L-cysteine S-palmitoylation)|go:"GO:0018345"(protein palmitoylation)|go:"GO:0019706"(protein-cysteine S-palmitoyltransferase activity)|go:"GO:0030176"(integral component of endoplasmic reticulum membrane)|go:"GO:0031211"(endoplasmic reticulum palmitoyltransferase complex)|go:"GO:0032541"(cortical endoplasmic reticulum)|go:"GO:0097038"(perinuclear endoplasmic reticulum)|interpro:IPR001594(Zinc finger, DHHC-type)	refseq:NP_014531.1|sgd:S000005470|dip:DIP-7228N|mint:P41912|ensemblfungi:YOL110W(gene)|ensemblfungi:YOL110W_mRNA(transcript)|go:"GO:0002178"(palmitoyltransferase complex)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0006612"(protein targeting to membrane)|go:"GO:0018230"(peptidyl-L-cysteine S-palmitoylation)|go:"GO:0018345"(protein palmitoylation)|go:"GO:0031211"(endoplasmic reticulum palmitoyltransferase complex)|go:"GO:0042406"(extrinsic component of endoplasmic reticulum membrane)|interpro:IPR019383|reactome:R-SCE-6798695	-	-	-	figure legend:Fig. 3C|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	-	2016/10/06	2016/10/07	rogid:6LBri1qWafIHxliiafTXrbrZ0JQ559292	rogid:BQ1Foh34E0ylxN2XnzqzluZradc559292	rigid:fuE2HDCyE0x9G5b2DzCsg9KSBZA	false	flag tag:n-n	glutathione s tranferase tag:n-n	-	-	psi-mi:"MI:0705"(anti tag western blot)	psi-mi:"MI:0705"(anti tag western blot)