#ID(s) interactor A	ID(s) interactor B	Alt. ID(s) interactor A	Alt. ID(s) interactor B	Alias(es) interactor A	Alias(es) interactor B	Interaction detection method(s)	Publication 1st author(s)	Publication Identifier(s)	Taxid interactor A	Taxid interactor B	Interaction type(s)	Source database(s)	Interaction identifier(s)	Confidence value(s)	Expansion method(s)	Biological role(s) interactor A	Biological role(s) interactor B	Experimental role(s) interactor A	Experimental role(s) interactor B	Type(s) interactor A	Type(s) interactor B	Xref(s) interactor A	Xref(s) interactor B	Interaction Xref(s)	Annotation(s) interactor A	Annotation(s) interactor B	Interaction annotation(s)	Host organism(s)	Interaction parameter(s)	Creation date	Update date	Checksum(s) interactor A	Checksum(s) interactor B	Interaction Checksum(s)	Negative	Feature(s) interactor A	Feature(s) interactor B	Stoichiometry(s) interactor A	Stoichiometry(s) interactor B	Identification method participant A	Identification method participant B
uniprotkb:P09484	uniprotkb:P26152	intact:EBI-10686072	intact:EBI-10686157	psi-mi:achb2_chick(display_long)|uniprotkb:CHRNB2(gene name)|psi-mi:CHRNB2(display_short)|uniprotkb:Neuronal acetylcholine receptor non-alpha-1 chain(gene name synonym)	psi-mi:acha5_chick(display_long)|uniprotkb:CHRNA5(gene name)|psi-mi:CHRNA5(display_short)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Conroy et al. (1998)	imex:IM-23927|pubmed:9495803	taxid:9031(chick)|taxid:9031("Gallus gallus (Chicken)")	taxid:9031(chick)|taxid:9031("Gallus gallus (Chicken)")	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-10686417|imex:IM-23927-2	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR032932|refseq:NP_990144.1|interpro:IPR006202(Neurotransmitter-gated ion-channel ligand-binding)|interpro:IPR006201(Neurotransmitter-gated ion-channel)|interpro:IPR006029(Neurotransmitter-gated ion-channel transmembrane region)|interpro:IPR018000|interpro:IPR002394(Nicotinic acetylcholine receptor, N-terminal)|go:"GO:0004888"(transmembrane signaling receptor activity)|go:"GO:0005737"(cytoplasm)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0007165"(signal transduction)|go:"GO:0007268"(chemical synaptic transmission)|go:"GO:0022848"(acetylcholine-gated cation-selective channel activity)|go:"GO:0030424"(axon)|go:"GO:0030425"(dendrite)|go:"GO:0030594"(neurotransmitter receptor activity)|go:"GO:0034220"(ion transmembrane transport)|go:"GO:0042391"(regulation of membrane potential)|go:"GO:0043005"(neuron projection)|go:"GO:0043204"(perikaryon)|go:"GO:0045202"(synapse)|go:"GO:0045211"(postsynaptic membrane)|go:"GO:0050877"(nervous system process)|interpro:IPR036719|interpro:IPR036734	refseq:NP_989746.1|interpro:IPR006202(Neurotransmitter-gated ion-channel ligand-binding)|interpro:IPR006201(Neurotransmitter-gated ion-channel)|interpro:IPR006029(Neurotransmitter-gated ion-channel transmembrane region)|interpro:IPR018000|interpro:IPR002394(Nicotinic acetylcholine receptor, N-terminal)|go:"GO:0004888"(transmembrane signaling receptor activity)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0007165"(signal transduction)|go:"GO:0007268"(chemical synaptic transmission)|go:"GO:0007271"(synaptic transmission, cholinergic)|go:"GO:0022848"(acetylcholine-gated cation-selective channel activity)|go:"GO:0030594"(neurotransmitter receptor activity)|go:"GO:0034220"(ion transmembrane transport)|go:"GO:0035094"(response to nicotine)|go:"GO:0042391"(regulation of membrane potential)|go:"GO:0043005"(neuron projection)|go:"GO:0045202"(synapse)|go:"GO:0045211"(postsynaptic membrane)|go:"GO:0050877"(nervous system process)|interpro:IPR036719|interpro:IPR036734	-	-	-	figure legend:"Fig 6b (lanes 2 & 6)"|comment:"Initial immunodepletion of alpha4-containing receptors resulted in weak binding of alpha5 to beta2 (lane 6)."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:9031(chick-brain)|taxid:9031(Gallus gallus)	-	2015/02/27	2015/05/11	rogid:W8PeYpMt64vzQGyNy39VgQbSd889031	rogid:cKoywqHMQ+aI/IJUfCoqUuBflhY9031	intact-crc:114C3993F45A8B27|rigid:OrAmi5nFzsWUnL3pjiGtw6pET5c	false	-	-	-	-	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)
uniprotkb:P09484	uniprotkb:P26152	intact:EBI-10686072	intact:EBI-10686157	psi-mi:achb2_chick(display_long)|uniprotkb:CHRNB2(gene name)|psi-mi:CHRNB2(display_short)|uniprotkb:Neuronal acetylcholine receptor non-alpha-1 chain(gene name synonym)	psi-mi:acha5_chick(display_long)|uniprotkb:CHRNA5(gene name)|psi-mi:CHRNA5(display_short)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Conroy et al. (1998)	imex:IM-23927|pubmed:9495803	taxid:9031(chick)|taxid:9031("Gallus gallus (Chicken)")	taxid:9031(chick)|taxid:9031("Gallus gallus (Chicken)")	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-10686397|imex:IM-23927-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR032932|refseq:NP_990144.1|interpro:IPR006202(Neurotransmitter-gated ion-channel ligand-binding)|interpro:IPR006201(Neurotransmitter-gated ion-channel)|interpro:IPR006029(Neurotransmitter-gated ion-channel transmembrane region)|interpro:IPR018000|interpro:IPR002394(Nicotinic acetylcholine receptor, N-terminal)|go:"GO:0004888"(transmembrane signaling receptor activity)|go:"GO:0005737"(cytoplasm)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0007165"(signal transduction)|go:"GO:0007268"(chemical synaptic transmission)|go:"GO:0022848"(acetylcholine-gated cation-selective channel activity)|go:"GO:0030424"(axon)|go:"GO:0030425"(dendrite)|go:"GO:0030594"(neurotransmitter receptor activity)|go:"GO:0034220"(ion transmembrane transport)|go:"GO:0042391"(regulation of membrane potential)|go:"GO:0043005"(neuron projection)|go:"GO:0043204"(perikaryon)|go:"GO:0045202"(synapse)|go:"GO:0045211"(postsynaptic membrane)|go:"GO:0050877"(nervous system process)|interpro:IPR036719|interpro:IPR036734	refseq:NP_989746.1|interpro:IPR006202(Neurotransmitter-gated ion-channel ligand-binding)|interpro:IPR006201(Neurotransmitter-gated ion-channel)|interpro:IPR006029(Neurotransmitter-gated ion-channel transmembrane region)|interpro:IPR018000|interpro:IPR002394(Nicotinic acetylcholine receptor, N-terminal)|go:"GO:0004888"(transmembrane signaling receptor activity)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0007165"(signal transduction)|go:"GO:0007268"(chemical synaptic transmission)|go:"GO:0007271"(synaptic transmission, cholinergic)|go:"GO:0022848"(acetylcholine-gated cation-selective channel activity)|go:"GO:0030594"(neurotransmitter receptor activity)|go:"GO:0034220"(ion transmembrane transport)|go:"GO:0035094"(response to nicotine)|go:"GO:0042391"(regulation of membrane potential)|go:"GO:0043005"(neuron projection)|go:"GO:0045202"(synapse)|go:"GO:0045211"(postsynaptic membrane)|go:"GO:0050877"(nervous system process)|interpro:IPR036719|interpro:IPR036734	-	-	-	figure legend:"Fig 6b (lanes 1, 3, 4, 5, 7, 8)"|comment:"IPs were carried out with or without initial immunodepletion of alpha4-containing receptors. In the case of alpha4-deletion some alpha4 remained in the beta2 IP (lane 4) but binding of alpha5 to beta2 was much stronger (lane 8)."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:9031(chick-brain)|taxid:9031(Gallus gallus)	-	2015/02/27	2015/02/27	rogid:W8PeYpMt64vzQGyNy39VgQbSd889031	rogid:cKoywqHMQ+aI/IJUfCoqUuBflhY9031	intact-crc:11E3E7222BE8D21F|rigid:P3a9TkZwKbiiE7+rACz5DA713Wg	false	-	-	-	-	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)
uniprotkb:P09484	uniprotkb:P09482	intact:EBI-10686072	intact:EBI-10686088|ensembl:ENSGALP00000009302	psi-mi:achb2_chick(display_long)|uniprotkb:CHRNB2(gene name)|psi-mi:CHRNB2(display_short)|uniprotkb:Neuronal acetylcholine receptor non-alpha-1 chain(gene name synonym)	psi-mi:acha4_chick(display_long)|uniprotkb:CHRNA4(gene name)|psi-mi:CHRNA4(display_short)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Conroy et al. (1998)	imex:IM-23927|pubmed:9495803	taxid:9031(chick)|taxid:9031("Gallus gallus (Chicken)")	taxid:9031(chick)|taxid:9031("Gallus gallus (Chicken)")	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-10686397|imex:IM-23927-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR032932|refseq:NP_990144.1|interpro:IPR006202(Neurotransmitter-gated ion-channel ligand-binding)|interpro:IPR006201(Neurotransmitter-gated ion-channel)|interpro:IPR006029(Neurotransmitter-gated ion-channel transmembrane region)|interpro:IPR018000|interpro:IPR002394(Nicotinic acetylcholine receptor, N-terminal)|go:"GO:0004888"(transmembrane signaling receptor activity)|go:"GO:0005737"(cytoplasm)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0007165"(signal transduction)|go:"GO:0007268"(chemical synaptic transmission)|go:"GO:0022848"(acetylcholine-gated cation-selective channel activity)|go:"GO:0030424"(axon)|go:"GO:0030425"(dendrite)|go:"GO:0030594"(neurotransmitter receptor activity)|go:"GO:0034220"(ion transmembrane transport)|go:"GO:0042391"(regulation of membrane potential)|go:"GO:0043005"(neuron projection)|go:"GO:0043204"(perikaryon)|go:"GO:0045202"(synapse)|go:"GO:0045211"(postsynaptic membrane)|go:"GO:0050877"(nervous system process)|interpro:IPR036719|interpro:IPR036734	refseq:NP_990145.1|ensembl:ENSGALG00000005801(gene)|ensembl:ENSGALT00000009316(transcript)|go:"GO:0001666"(response to hypoxia)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0005892"(acetylcholine-gated channel complex)|go:"GO:0006281"(DNA repair)|go:"GO:0006979"(response to oxidative stress)|go:"GO:0007165"(signal transduction)|go:"GO:0007268"(chemical synaptic transmission)|go:"GO:0007271"(synaptic transmission, cholinergic)|go:"GO:0015464"(acetylcholine receptor activity)|go:"GO:0022848"(acetylcholine-gated cation-selective channel activity)|go:"GO:0030594"(neurotransmitter receptor activity)|go:"GO:0034220"(ion transmembrane transport)|go:"GO:0035094"(response to nicotine)|go:"GO:0035095"(behavioral response to nicotine)|go:"GO:0042391"(regulation of membrane potential)|go:"GO:0043005"(neuron projection)|go:"GO:0045202"(synapse)|go:"GO:0045211"(postsynaptic membrane)|go:"GO:0050877"(nervous system process)|go:"GO:0050890"(cognition)|interpro:IPR002394(Nicotinic acetylcholine receptor, N-terminal)|interpro:IPR006029(Neurotransmitter-gated ion-channel transmembrane region)|interpro:IPR006201(Neurotransmitter-gated ion-channel)|interpro:IPR006202(Neurotransmitter-gated ion-channel ligand-binding)|interpro:IPR018000|interpro:IPR036719|interpro:IPR036734	-	-	-	figure legend:"Fig 6b (lanes 1, 3, 4, 5, 7, 8)"|comment:"IPs were carried out with or without initial immunodepletion of alpha4-containing receptors. In the case of alpha4-deletion some alpha4 remained in the beta2 IP (lane 4) but binding of alpha5 to beta2 was much stronger (lane 8)."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:9031(chick-brain)|taxid:9031(Gallus gallus)	-	2015/02/27	2015/02/27	rogid:W8PeYpMt64vzQGyNy39VgQbSd889031	rogid:6Zi4QLM752J2YldSj21kGxfeEp09031	intact-crc:11E3E7222BE8D21F|rigid:P3a9TkZwKbiiE7+rACz5DA713Wg	false	-	-	-	-	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)