#ID(s) interactor A	ID(s) interactor B	Alt. ID(s) interactor A	Alt. ID(s) interactor B	Alias(es) interactor A	Alias(es) interactor B	Interaction detection method(s)	Publication 1st author(s)	Publication Identifier(s)	Taxid interactor A	Taxid interactor B	Interaction type(s)	Source database(s)	Interaction identifier(s)	Confidence value(s)	Expansion method(s)	Biological role(s) interactor A	Biological role(s) interactor B	Experimental role(s) interactor A	Experimental role(s) interactor B	Type(s) interactor A	Type(s) interactor B	Xref(s) interactor A	Xref(s) interactor B	Interaction Xref(s)	Annotation(s) interactor A	Annotation(s) interactor B	Interaction annotation(s)	Host organism(s)	Interaction parameter(s)	Creation date	Update date	Checksum(s) interactor A	Checksum(s) interactor B	Interaction Checksum(s)	Negative	Feature(s) interactor A	Feature(s) interactor B	Stoichiometry(s) interactor A	Stoichiometry(s) interactor B	Identification method participant A	Identification method participant B
uniprotkb:Q9RPX5	uniprotkb:Q9RPX9	intact:EBI-7022388|ensemblbacteria:AEM19552|intact:MINT-8194197|uniprotkb:G0KEQ8|uniprotkb:Q8FXK6	intact:EBI-11178687|uniprotkb:G0KER2|uniprotkb:Q8FXK5|ensemblbacteria:AEM19556	psi-mi:virba_brusu(display_long)|uniprotkb:BS1330_II0060(locus name)|uniprotkb:virB10(gene name)|psi-mi:virB10(display_short)|uniprotkb:BRA0060(locus name)	psi-mi:virb6_brusu(display_long)|uniprotkb:virB6(gene name)|psi-mi:virB6(display_short)|uniprotkb:BRA0064(locus name)|uniprotkb:BS1330_II0064(locus name)	psi-mi:"MI:0018"(two hybrid)	Villamil et al. (2015)	pubmed:26071378|imex:IM-24520	taxid:204722(brusu)|taxid:204722(brusu)	taxid:204722(brusu)|taxid:204722(brusu)	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0471"(MINT)	intact:EBI-11178684|imex:IM-24520-1	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	dip:DIP-61161N|interpro:IPR042217|interpro:IPR005498(Bacterial conjugation TrbI-like protein)|ensemblbacteria:AEM19552(transcript)|ensemblbacteria:BS1330_II0060(gene)|go:"GO:0005886"(plasma membrane)|go:"GO:0016021"(integral component of membrane)|refseq:WP_004689962.1	refseq:WP_002969266.1|interpro:IPR007688(Conjugal transfer, TrbL/VirB6)|ensemblbacteria:AEM19556(transcript)|ensemblbacteria:BS1330_II0064(gene)|go:"GO:0005886"(plasma membrane)|go:"GO:0016021"(integral component of membrane)|go:"GO:0030255"(protein secretion by the type IV secretion system)	-	comment:mint	-	figure legend:f4|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	-	2015/09/16	2015/09/16	rogid:R3tbNGd2fX4ffjvYZOvyzCc+wXE204722	rogid:lxxTl54x41FGnk8l19RmgJPWbc4204722	rigid:uFzpV5nECa08Y9We5YVt7zMMuVI	false	fusion protein:?-?	binding-associated region:1-168|fusion protein:?-?	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
uniprotkb:Q9RPX5	uniprotkb:Q7CEG3	intact:EBI-7022388|ensemblbacteria:AEM19552|intact:MINT-8194197|uniprotkb:G0KEQ8|uniprotkb:Q8FXK6	intact:EBI-6407073|ensemblbacteria:AEM19554|uniprotkb:Q7BQL4|uniprotkb:G0KER0	psi-mi:virba_brusu(display_long)|uniprotkb:BS1330_II0060(locus name)|uniprotkb:virB10(gene name)|psi-mi:virB10(display_short)|uniprotkb:BRA0060(locus name)	psi-mi:virb8_brusu(display_long)|uniprotkb:BRA0062(locus name)|uniprotkb:BS1330_II0062(locus name)|uniprotkb:virB8(gene name)|psi-mi:virB8(display_short)	psi-mi:"MI:0018"(two hybrid)	Villamil et al. (2015)	pubmed:26071378|imex:IM-24520	taxid:204722(brusu)|taxid:204722(brusu)	taxid:204722(brusu)|taxid:204722(brusu)	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0471"(MINT)	intact:EBI-11178713|imex:IM-24520-3	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	dip:DIP-61161N|interpro:IPR042217|interpro:IPR005498(Bacterial conjugation TrbI-like protein)|ensemblbacteria:AEM19552(transcript)|ensemblbacteria:BS1330_II0060(gene)|go:"GO:0005886"(plasma membrane)|go:"GO:0016021"(integral component of membrane)|refseq:WP_004689962.1	ensemblbacteria:AEM19554(transcript)|ensemblbacteria:BS1330_II0062(gene)|go:"GO:0005886"(plasma membrane)|go:"GO:0016021"(integral component of membrane)|go:"GO:0030255"(protein secretion by the type IV secretion system)|go:"GO:0042802"(identical protein binding)|interpro:IPR007430(VirB8)|interpro:IPR026264|interpro:IPR032710|rcsb pdb:2BHM|rcsb pdb:4AKY|rcsb pdb:4AKZ|rcsb pdb:5JBS|refseq:WP_002966517.1|dip:DIP-61163N	-	comment:mint	-	figure legend:f4|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	-	2015/09/16	2015/09/16	rogid:R3tbNGd2fX4ffjvYZOvyzCc+wXE204722	rogid:L6fE8kfdp8j/Ru+VI8vyjTHd6Kw204722	rigid:Rq1kZIDhnbzrTmAXrV1zqPS2EXI	false	fusion protein:?-?	fusion protein:?-?	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
uniprotkb:Q9RPX5	uniprotkb:Q9RPX9	intact:EBI-7022388|ensemblbacteria:AEM19552|intact:MINT-8194197|uniprotkb:G0KEQ8|uniprotkb:Q8FXK6	intact:EBI-11178687|uniprotkb:G0KER2|uniprotkb:Q8FXK5|ensemblbacteria:AEM19556	psi-mi:virba_brusu(display_long)|uniprotkb:BS1330_II0060(locus name)|uniprotkb:virB10(gene name)|psi-mi:virB10(display_short)|uniprotkb:BRA0060(locus name)	psi-mi:virb6_brusu(display_long)|uniprotkb:virB6(gene name)|psi-mi:virB6(display_short)|uniprotkb:BRA0064(locus name)|uniprotkb:BS1330_II0064(locus name)	psi-mi:"MI:0084"(phage display)	Villamil et al. (2015)	pubmed:26071378|imex:IM-24520	taxid:204722(brusu)|taxid:204722(brusu)	taxid:204722(brusu)|taxid:204722(brusu)	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0471"(MINT)	intact:EBI-11178731|imex:IM-24520-5	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	dip:DIP-61161N|interpro:IPR042217|interpro:IPR005498(Bacterial conjugation TrbI-like protein)|ensemblbacteria:AEM19552(transcript)|ensemblbacteria:BS1330_II0060(gene)|go:"GO:0005886"(plasma membrane)|go:"GO:0016021"(integral component of membrane)|refseq:WP_004689962.1	refseq:WP_002969266.1|interpro:IPR007688(Conjugal transfer, TrbL/VirB6)|ensemblbacteria:AEM19556(transcript)|ensemblbacteria:BS1330_II0064(gene)|go:"GO:0005886"(plasma membrane)|go:"GO:0016021"(integral component of membrane)|go:"GO:0030255"(protein secretion by the type IV secretion system)	-	comment:mint	-	figure legend:f3|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)")	-	2015/09/16	2015/09/16	rogid:R3tbNGd2fX4ffjvYZOvyzCc+wXE204722	rogid:lxxTl54x41FGnk8l19RmgJPWbc4204722	rigid:uFzpV5nECa08Y9We5YVt7zMMuVI	false	-	-	-	-	psi-mi:"MI:0078"(nucleotide sequence identification)	psi-mi:"MI:0078"(nucleotide sequence identification)
uniprotkb:Q9RPX5	uniprotkb:Q9RPX9	intact:EBI-7022388|ensemblbacteria:AEM19552|intact:MINT-8194197|uniprotkb:G0KEQ8|uniprotkb:Q8FXK6	intact:EBI-11178687|uniprotkb:G0KER2|uniprotkb:Q8FXK5|ensemblbacteria:AEM19556	psi-mi:virba_brusu(display_long)|uniprotkb:BS1330_II0060(locus name)|uniprotkb:virB10(gene name)|psi-mi:virB10(display_short)|uniprotkb:BRA0060(locus name)	psi-mi:virb6_brusu(display_long)|uniprotkb:virB6(gene name)|psi-mi:virB6(display_short)|uniprotkb:BRA0064(locus name)|uniprotkb:BS1330_II0064(locus name)	psi-mi:"MI:0055"(fluorescent resonance energy transfer)	Villamil et al. (2015)	pubmed:26071378|imex:IM-24520	taxid:204722(brusu)|taxid:204722(brusu)	taxid:204722(brusu)|taxid:204722(brusu)	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0471"(MINT)	intact:EBI-11178748|imex:IM-24520-7	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0583"(fluorescence donor)	psi-mi:"MI:0584"(fluorescence acceptor)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	dip:DIP-61161N|interpro:IPR042217|interpro:IPR005498(Bacterial conjugation TrbI-like protein)|ensemblbacteria:AEM19552(transcript)|ensemblbacteria:BS1330_II0060(gene)|go:"GO:0005886"(plasma membrane)|go:"GO:0016021"(integral component of membrane)|refseq:WP_004689962.1	refseq:WP_002969266.1|interpro:IPR007688(Conjugal transfer, TrbL/VirB6)|ensemblbacteria:AEM19556(transcript)|ensemblbacteria:BS1330_II0064(gene)|go:"GO:0005886"(plasma membrane)|go:"GO:0016021"(integral component of membrane)|go:"GO:0030255"(protein secretion by the type IV secretion system)	-	comment:mint	-	figure legend:f5|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	kd:4.0x10^-6(molar)	2015/09/16	2015/09/18	rogid:R3tbNGd2fX4ffjvYZOvyzCc+wXE204722	rogid:lxxTl54x41FGnk8l19RmgJPWbc4204722	rigid:uFzpV5nECa08Y9We5YVt7zMMuVI	false	fluorophore:?-?	fluorophore:?-?|binding-associated region:10-33	-	-	psi-mi:"MI:0867"(tag visualisation by fluorescence)	psi-mi:"MI:0867"(tag visualisation by fluorescence)
uniprotkb:Q9RPX5	uniprotkb:Q7CEG3	intact:EBI-7022388|ensemblbacteria:AEM19552|intact:MINT-8194197|uniprotkb:G0KEQ8|uniprotkb:Q8FXK6	intact:EBI-6407073|ensemblbacteria:AEM19554|uniprotkb:Q7BQL4|uniprotkb:G0KER0	psi-mi:virba_brusu(display_long)|uniprotkb:BS1330_II0060(locus name)|uniprotkb:virB10(gene name)|psi-mi:virB10(display_short)|uniprotkb:BRA0060(locus name)	psi-mi:virb8_brusu(display_long)|uniprotkb:BRA0062(locus name)|uniprotkb:BS1330_II0062(locus name)|uniprotkb:virB8(gene name)|psi-mi:virB8(display_short)	psi-mi:"MI:0055"(fluorescent resonance energy transfer)	Villamil et al. (2015)	pubmed:26071378|imex:IM-24520	taxid:204722(brusu)|taxid:204722(brusu)	taxid:204722(brusu)|taxid:204722(brusu)	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0471"(MINT)	intact:EBI-11179240|imex:IM-24520-9	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0583"(fluorescence donor)	psi-mi:"MI:0584"(fluorescence acceptor)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	dip:DIP-61161N|interpro:IPR042217|interpro:IPR005498(Bacterial conjugation TrbI-like protein)|ensemblbacteria:AEM19552(transcript)|ensemblbacteria:BS1330_II0060(gene)|go:"GO:0005886"(plasma membrane)|go:"GO:0016021"(integral component of membrane)|refseq:WP_004689962.1	ensemblbacteria:AEM19554(transcript)|ensemblbacteria:BS1330_II0062(gene)|go:"GO:0005886"(plasma membrane)|go:"GO:0016021"(integral component of membrane)|go:"GO:0030255"(protein secretion by the type IV secretion system)|go:"GO:0042802"(identical protein binding)|interpro:IPR007430(VirB8)|interpro:IPR026264|interpro:IPR032710|rcsb pdb:2BHM|rcsb pdb:4AKY|rcsb pdb:4AKZ|rcsb pdb:5JBS|refseq:WP_002966517.1|dip:DIP-61163N	-	comment:mint	-	figure legend:f6|comment:"Considering that the N-terminal peptide interacts with VirB10,  the central VirB6 peptide interacts with VirB8 [7] and that VirB8  also interacts with VirB10 [24], we reasoned that these interactions  may impact each other. To test this possibility, we conducted FRET  assays with donor and acceptor pairs switched accordingly. VirB10  was labeled with the donor fluorophore (Alexa fluor 488) while  VirB8 was labeled with the acceptor fluorophore (Alexa fluor  546). The titration curve indicated a KD value of 0.5 lM, which is  in the same range of our previous data [24] (Fig. 6A). Next, the efficiency  of energy transfer was assessed in the presence of unlabeled  VirB6 N-terminal peptide (Fig. 6A) and of unlabeled VirB6 central  peptide (Fig. 6B). Neither of these peptides affected the energy  transfer between VirB10 and VirB8 suggesting that none of the  two VirB6-derived peptides affects the interaction between VirB8  and VirB10."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	kd:5.0x10^-6(molar)	2015/09/16	2015/09/18	rogid:R3tbNGd2fX4ffjvYZOvyzCc+wXE204722	rogid:L6fE8kfdp8j/Ru+VI8vyjTHd6Kw204722	rigid:Rq1kZIDhnbzrTmAXrV1zqPS2EXI	false	fluorophore:?-?	fluorophore:?-?	-	-	psi-mi:"MI:0867"(tag visualisation by fluorescence)	psi-mi:"MI:0867"(tag visualisation by fluorescence)