#ID(s) interactor A ID(s) interactor B Alt. ID(s) interactor A Alt. ID(s) interactor B Alias(es) interactor A Alias(es) interactor B Interaction detection method(s) Publication 1st author(s) Publication Identifier(s) Taxid interactor A Taxid interactor B Interaction type(s) Source database(s) Interaction identifier(s) Confidence value(s) Expansion method(s) Biological role(s) interactor A Biological role(s) interactor B Experimental role(s) interactor A Experimental role(s) interactor B Type(s) interactor A Type(s) interactor B Xref(s) interactor A Xref(s) interactor B Interaction Xref(s) Annotation(s) interactor A Annotation(s) interactor B Interaction annotation(s) Host organism(s) Interaction parameter(s) Creation date Update date Checksum(s) interactor A Checksum(s) interactor B Interaction Checksum(s) Negative Feature(s) interactor A Feature(s) interactor B Stoichiometry(s) interactor A Stoichiometry(s) interactor B Identification method participant A Identification method participant B uniprotkb:Q70VB1 uniprotkb:Q70VB1 intact:EBI-10711112|ensembl:ENSRNOP00000000451 intact:EBI-10711112|ensembl:ENSRNOP00000000451 psi-mi:gpc6a_rat(display_long)|uniprotkb:Gprc6a(gene name)|psi-mi:Gprc6a(display_short) psi-mi:gpc6a_rat(display_long)|uniprotkb:Gprc6a(gene name)|psi-mi:Gprc6a(display_short) psi-mi:"MI:0510"(homogeneous time resolved fluorescence) Nørskov-Lauritsen et al. (2015) imex:IM-24020|pubmed:25617829 taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0471"(MINT) intact:EBI-10711098|imex:IM-24020-1 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0583"(fluorescence donor) psi-mi:"MI:0584"(fluorescence acceptor) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) refseq:NP_001258035.1|rgd:735077|interpro:IPR000337(GPCR, family 3)|interpro:IPR001828(Extracellular ligand-binding receptor)|interpro:IPR011500(GPCR, family 3, nine cysteines region)|interpro:IPR017978|interpro:IPR017979|interpro:IPR028082|interpro:IPR038550|reactome:R-RNO-416476|reactome:R-RNO-420499|ensembl:ENSRNOG00000000401(gene)|ensembl:ENSRNOT00000000451(transcript)|go:"GO:0004930"(G protein-coupled receptor activity)|go:"GO:0005886"(plasma membrane)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0043200"(response to amino acid)|interpro:IPR000068(GPCR, family 3, extracellular calcium-sensing receptor-related) refseq:NP_001258035.1|rgd:735077|interpro:IPR000337(GPCR, family 3)|interpro:IPR001828(Extracellular ligand-binding receptor)|interpro:IPR011500(GPCR, family 3, nine cysteines region)|interpro:IPR017978|interpro:IPR017979|interpro:IPR028082|interpro:IPR038550|reactome:R-RNO-416476|reactome:R-RNO-420499|ensembl:ENSRNOG00000000401(gene)|ensembl:ENSRNOT00000000451(transcript)|go:"GO:0004930"(G protein-coupled receptor activity)|go:"GO:0005886"(plasma membrane)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0043200"(response to amino acid)|interpro:IPR000068(GPCR, family 3, extracellular calcium-sensing receptor-related) - - - figure legend:F4|comment:"To determine if rGPRC6A is indeed a dimer an HTRF assay was conducted using an anti-HA antibody labeled with a donor fluorophore and an anti-c-myc labeled acceptor fluorophore (Fig. 4A). The results clearly show that rGPRC6A is capable of homodimerizing as co-expression of HA- and c-myc-tagged rGPRC6A receptors generates a FRET signal, whereas co-expression of c-myc-rGPRC6A with the unrelated HA-tagged 5-HT2A receptor did not generate a FRET signal (Fig. 4B). These experiments were performed on intact cells demonstrating that the rGPRC6A receptors dimerize on the cell surface."|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:9606(human-293t)|taxid:9606(Homo sapiens 293 cells transformed with SV40 large T antigen) - 2015/03/19 2015/03/19 rogid:McIs6t3IhgMh+VKR2anFCmTfImc10116 rogid:McIs6t3IhgMh+VKR2anFCmTfImc10116 rigid:VqF4YUaATCMT9p8KKKZjxZJnFPw false ha tag:?-? myc tag:?-? - - psi-mi:"MI:0867"(tag visualisation by fluorescence) psi-mi:"MI:0867"(tag visualisation by fluorescence)