#ID(s) interactor A	ID(s) interactor B	Alt. ID(s) interactor A	Alt. ID(s) interactor B	Alias(es) interactor A	Alias(es) interactor B	Interaction detection method(s)	Publication 1st author(s)	Publication Identifier(s)	Taxid interactor A	Taxid interactor B	Interaction type(s)	Source database(s)	Interaction identifier(s)	Confidence value(s)	Expansion method(s)	Biological role(s) interactor A	Biological role(s) interactor B	Experimental role(s) interactor A	Experimental role(s) interactor B	Type(s) interactor A	Type(s) interactor B	Xref(s) interactor A	Xref(s) interactor B	Interaction Xref(s)	Annotation(s) interactor A	Annotation(s) interactor B	Interaction annotation(s)	Host organism(s)	Interaction parameter(s)	Creation date	Update date	Checksum(s) interactor A	Checksum(s) interactor B	Interaction Checksum(s)	Negative	Feature(s) interactor A	Feature(s) interactor B	Stoichiometry(s) interactor A	Stoichiometry(s) interactor B	Identification method participant A	Identification method participant B
uniprotkb:Q9FUK4	uniprotkb:Q9FUK4	intact:EBI-10191057|ensemblplants:KRH30929	intact:EBI-10191057|ensemblplants:KRH30929	psi-mi:q9fuk4_soybn(display_long)|uniprotkb:GLYMA_11G215500(orf name)|uniprotkb:548082(gene name)|psi-mi:548082(display_short)	psi-mi:q9fuk4_soybn(display_long)|uniprotkb:GLYMA_11G215500(orf name)|uniprotkb:548082(gene name)|psi-mi:548082(display_short)	psi-mi:"MI:0028"(cosedimentation in solution)	Masalkar et al. (2015)	pubmed:25497014|imex:IM-23836	taxid:3847(soybn)|taxid:3847("Glycine max (Soybean)")	taxid:3847(soybn)|taxid:3847("Glycine max (Soybean)")	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0471"(MINT)	intact:EBI-10189815|imex:IM-23836-1	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	interpro:IPR008147(Glutamine synthetase, beta-Grasp)|interpro:IPR014746(Glutamine synthetase/guanido kinase, catalytic region)|interpro:IPR008146(Glutamine synthetase, catalytic region)|interpro:IPR027303|interpro:IPR027302|interpro:IPR036651|ensemblplants:GLYMA_11G215500(gene)|ensemblplants:KRH30929(transcript)|go:"GO:0004356"(glutamate-ammonia ligase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005737"(cytoplasm)|go:"GO:0006542"(glutamine biosynthetic process)|refseq:NP_001238531.1	interpro:IPR008147(Glutamine synthetase, beta-Grasp)|interpro:IPR014746(Glutamine synthetase/guanido kinase, catalytic region)|interpro:IPR008146(Glutamine synthetase, catalytic region)|interpro:IPR027303|interpro:IPR027302|interpro:IPR036651|ensemblplants:GLYMA_11G215500(gene)|ensemblplants:KRH30929(transcript)|go:"GO:0004356"(glutamate-ammonia ligase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005737"(cytoplasm)|go:"GO:0006542"(glutamine biosynthetic process)|refseq:NP_001238531.1	-	-	-	figure legend:f4b t1 sf3a|comment:"The  molecular weight calculated from equilibrium AUC for GS1c1 is  499.28kDa whereas GS1b1 is 409.55kDa (Table 1), supporting the  results of velocity AUC. Considering the monomeric molecular  weight of both isoforms, the 499.28kDa GS1c1 would represent  an oligomer of 12 subunits, which is distinct from the 409.55kDa GS1b1 which would represent a decameric enzyme similar to  maize GS1a enzyme"|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2015/02/13	2015/02/13	rogid:e/EOcmGA5yC+og4j/dJq7dzZ9OM3847	rogid:e/EOcmGA5yC+og4j/dJq7dzZ9OM3847	intact-crc:15CE0FFEF9151602|rigid:vfdApHtuLYWay7+421XWZpBSMRs	false	-	-	10	0	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
uniprotkb:O82560	uniprotkb:O82560	intact:EBI-10192111|ensemblplants:KRH17323	intact:EBI-10192111|ensemblplants:KRH17323	psi-mi:glna2_soybn(display_long)|uniprotkb:GS1-2(gene name synonym)|uniprotkb:Glutamate--ammonia ligase(gene name synonym)	psi-mi:glna2_soybn(display_long)|uniprotkb:GS1-2(gene name synonym)|uniprotkb:Glutamate--ammonia ligase(gene name synonym)	psi-mi:"MI:0028"(cosedimentation in solution)	Masalkar et al. (2015)	pubmed:25497014|imex:IM-23836	taxid:3847(soybn)|taxid:3847("Glycine max (Soybean)")	taxid:3847(soybn)|taxid:3847("Glycine max (Soybean)")	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0471"(MINT)	intact:EBI-10192108|imex:IM-23836-2	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:XP_003544980.1|interpro:IPR008147(Glutamine synthetase, beta-Grasp)|interpro:IPR014746(Glutamine synthetase/guanido kinase, catalytic region)|interpro:IPR008146(Glutamine synthetase, catalytic region)|interpro:IPR027303|interpro:IPR027302|interpro:IPR036651|ensemblplants:GLYMA_14G213300(gene)|ensemblplants:KRH17323(transcript)|go:"GO:0004356"(glutamate-ammonia ligase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005737"(cytoplasm)|go:"GO:0006542"(glutamine biosynthetic process)|go:"GO:0042802"(identical protein binding)	refseq:XP_003544980.1|interpro:IPR008147(Glutamine synthetase, beta-Grasp)|interpro:IPR014746(Glutamine synthetase/guanido kinase, catalytic region)|interpro:IPR008146(Glutamine synthetase, catalytic region)|interpro:IPR027303|interpro:IPR027302|interpro:IPR036651|ensemblplants:GLYMA_14G213300(gene)|ensemblplants:KRH17323(transcript)|go:"GO:0004356"(glutamate-ammonia ligase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005737"(cytoplasm)|go:"GO:0006542"(glutamine biosynthetic process)|go:"GO:0042802"(identical protein binding)	-	-	-	figure legend:f4b t1 sf3b|comment:"The  molecular weight calculated from equilibrium AUC for GS1c1 is  499.28kDa whereas GS1b1 is 409.55kDa (Table 1), supporting the  results of velocity AUC. Considering the monomeric molecular  weight of both isoforms, the 499.28kDa GS1c1 would represent  an oligomer of 12 subunits, which is distinct from the 409.55kDa GS1b1 which would represent a decameric enzyme similar to  maize GS1a enzyme"|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2015/02/13	2015/02/13	rogid:UHjD7r35272NQcQM+xLEbDyxiHQ3847	rogid:UHjD7r35272NQcQM+xLEbDyxiHQ3847	intact-crc:AE5795F52ADD6DAC|rigid:LgPds+0Ef8bNIKbOC08tflC+9kY	false	-	-	12	0	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
uniprotkb:O82560	uniprotkb:O82560	intact:EBI-10192111|ensemblplants:KRH17323	intact:EBI-10192111|ensemblplants:KRH17323	psi-mi:glna2_soybn(display_long)|uniprotkb:GS1-2(gene name synonym)|uniprotkb:Glutamate--ammonia ligase(gene name synonym)	psi-mi:glna2_soybn(display_long)|uniprotkb:GS1-2(gene name synonym)|uniprotkb:Glutamate--ammonia ligase(gene name synonym)	psi-mi:"MI:0404"(comigration in non denaturing gel electrophoresis)	Masalkar et al. (2015)	pubmed:25497014|imex:IM-23836	taxid:3847(soybn)|taxid:3847("Glycine max (Soybean)")	taxid:3847(soybn)|taxid:3847("Glycine max (Soybean)")	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0471"(MINT)	intact:EBI-10195234|imex:IM-23836-3	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:XP_003544980.1|interpro:IPR008147(Glutamine synthetase, beta-Grasp)|interpro:IPR014746(Glutamine synthetase/guanido kinase, catalytic region)|interpro:IPR008146(Glutamine synthetase, catalytic region)|interpro:IPR027303|interpro:IPR027302|interpro:IPR036651|ensemblplants:GLYMA_14G213300(gene)|ensemblplants:KRH17323(transcript)|go:"GO:0004356"(glutamate-ammonia ligase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005737"(cytoplasm)|go:"GO:0006542"(glutamine biosynthetic process)|go:"GO:0042802"(identical protein binding)	refseq:XP_003544980.1|interpro:IPR008147(Glutamine synthetase, beta-Grasp)|interpro:IPR014746(Glutamine synthetase/guanido kinase, catalytic region)|interpro:IPR008146(Glutamine synthetase, catalytic region)|interpro:IPR027303|interpro:IPR027302|interpro:IPR036651|ensemblplants:GLYMA_14G213300(gene)|ensemblplants:KRH17323(transcript)|go:"GO:0004356"(glutamate-ammonia ligase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005737"(cytoplasm)|go:"GO:0006542"(glutamine biosynthetic process)|go:"GO:0042802"(identical protein binding)	-	-	-	figure legend:f1b f3d|comment:"After exposure to oxidizing conditions (air oxidation or  H2O2 incubation), purified GS1c1C159S and GS1c1 were analyzed  by non-reducing SDS–PAGE to determine the effect of oxidation  on oligomerization. In contrast to wild type GS1c1, high molecular  weight oligomeric species were not observed in the GS1c1C159S  mutant under oxidizing conditions (Fig. 3D)."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2015/02/13	2015/02/13	rogid:UHjD7r35272NQcQM+xLEbDyxiHQ3847	rogid:UHjD7r35272NQcQM+xLEbDyxiHQ3847	intact-crc:07A22560A851D6FB|rigid:gzS7Cpr6er4mK0901iml0ss67as	false	mutation disrupting interaction:159-159	mutation disrupting interaction:159-159	-	-	psi-mi:"MI:0816"(molecular weight estimation by staining)	psi-mi:"MI:0816"(molecular weight estimation by staining)
uniprotkb:O82560	uniprotkb:O82560	intact:EBI-10192111|ensemblplants:KRH17323	intact:EBI-10192111|ensemblplants:KRH17323	psi-mi:glna2_soybn(display_long)|uniprotkb:GS1-2(gene name synonym)|uniprotkb:Glutamate--ammonia ligase(gene name synonym)	psi-mi:glna2_soybn(display_long)|uniprotkb:GS1-2(gene name synonym)|uniprotkb:Glutamate--ammonia ligase(gene name synonym)	psi-mi:"MI:0276"(blue native page)	Masalkar et al. (2015)	pubmed:25497014|imex:IM-23836	taxid:3847(soybn)|taxid:3847("Glycine max (Soybean)")	taxid:3847(soybn)|taxid:3847("Glycine max (Soybean)")	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0471"(MINT)	intact:EBI-10198555|imex:IM-23836-4	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:XP_003544980.1|interpro:IPR008147(Glutamine synthetase, beta-Grasp)|interpro:IPR014746(Glutamine synthetase/guanido kinase, catalytic region)|interpro:IPR008146(Glutamine synthetase, catalytic region)|interpro:IPR027303|interpro:IPR027302|interpro:IPR036651|ensemblplants:GLYMA_14G213300(gene)|ensemblplants:KRH17323(transcript)|go:"GO:0004356"(glutamate-ammonia ligase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005737"(cytoplasm)|go:"GO:0006542"(glutamine biosynthetic process)|go:"GO:0042802"(identical protein binding)	refseq:XP_003544980.1|interpro:IPR008147(Glutamine synthetase, beta-Grasp)|interpro:IPR014746(Glutamine synthetase/guanido kinase, catalytic region)|interpro:IPR008146(Glutamine synthetase, catalytic region)|interpro:IPR027303|interpro:IPR027302|interpro:IPR036651|ensemblplants:GLYMA_14G213300(gene)|ensemblplants:KRH17323(transcript)|go:"GO:0004356"(glutamate-ammonia ligase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005737"(cytoplasm)|go:"GO:0006542"(glutamine biosynthetic process)|go:"GO:0042802"(identical protein binding)	-	-	-	figure legend:f4a|comment:"Comparison of the two GS1 isoforms by native PAGE showed  differences in electrophoretic mobility. GS1c1 has a single band  with a reduced electrophoretic mobility compared to the major  band in GS1b1 (Fig. 4A). A minor species of GS1b1 with a similar  electrophoretic mobility as GS1c1 was also observed, suggesting  the presence of a small amount of a second oligomeric species with  similar properties to the c isoform. Nevertheless, the results show  fundamental differences in the physicochemical properties of the  two isoforms."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2015/02/13	2015/02/13	rogid:UHjD7r35272NQcQM+xLEbDyxiHQ3847	rogid:UHjD7r35272NQcQM+xLEbDyxiHQ3847	intact-crc:A2EBCC344CC5886B|rigid:gzS7Cpr6er4mK0901iml0ss67as	false	-	-	-	-	psi-mi:"MI:0818"(molecular weight estimation by coomasie staining)	psi-mi:"MI:0818"(molecular weight estimation by coomasie staining)
uniprotkb:O82560	uniprotkb:O82560	intact:EBI-10192111|ensemblplants:KRH17323	intact:EBI-10192111|ensemblplants:KRH17323	psi-mi:glna2_soybn(display_long)|uniprotkb:GS1-2(gene name synonym)|uniprotkb:Glutamate--ammonia ligase(gene name synonym)	psi-mi:glna2_soybn(display_long)|uniprotkb:GS1-2(gene name synonym)|uniprotkb:Glutamate--ammonia ligase(gene name synonym)	psi-mi:"MI:0071"(molecular sieving)	Masalkar et al. (2015)	pubmed:25497014|imex:IM-23836	taxid:3847(soybn)|taxid:3847("Glycine max (Soybean)")	taxid:3847(soybn)|taxid:3847("Glycine max (Soybean)")	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0471"(MINT)	intact:EBI-10205079|imex:IM-23836-5	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:XP_003544980.1|interpro:IPR008147(Glutamine synthetase, beta-Grasp)|interpro:IPR014746(Glutamine synthetase/guanido kinase, catalytic region)|interpro:IPR008146(Glutamine synthetase, catalytic region)|interpro:IPR027303|interpro:IPR027302|interpro:IPR036651|ensemblplants:GLYMA_14G213300(gene)|ensemblplants:KRH17323(transcript)|go:"GO:0004356"(glutamate-ammonia ligase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005737"(cytoplasm)|go:"GO:0006542"(glutamine biosynthetic process)|go:"GO:0042802"(identical protein binding)	refseq:XP_003544980.1|interpro:IPR008147(Glutamine synthetase, beta-Grasp)|interpro:IPR014746(Glutamine synthetase/guanido kinase, catalytic region)|interpro:IPR008146(Glutamine synthetase, catalytic region)|interpro:IPR027303|interpro:IPR027302|interpro:IPR036651|ensemblplants:GLYMA_14G213300(gene)|ensemblplants:KRH17323(transcript)|go:"GO:0004356"(glutamate-ammonia ligase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005737"(cytoplasm)|go:"GO:0006542"(glutamine biosynthetic process)|go:"GO:0042802"(identical protein binding)	-	-	-	figure legend:sf2|comment:"The possibility that oligomerization was the result of an intersubunit  disulfide between cys92 and cys159, was investigated by  site-directed mutagenesis in which each cysteine residue was  replaced by serine. Of the two cys-to-ser mutants, only GS1-  c1C159S produced an active oligomeric protein (Supplementary  Fig. S2)."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2015/02/13	2015/02/13	rogid:UHjD7r35272NQcQM+xLEbDyxiHQ3847	rogid:UHjD7r35272NQcQM+xLEbDyxiHQ3847	intact-crc:20023D16015C61F7|rigid:gzS7Cpr6er4mK0901iml0ss67as	false	-	-	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)