#ID(s) interactor A	ID(s) interactor B	Alt. ID(s) interactor A	Alt. ID(s) interactor B	Alias(es) interactor A	Alias(es) interactor B	Interaction detection method(s)	Publication 1st author(s)	Publication Identifier(s)	Taxid interactor A	Taxid interactor B	Interaction type(s)	Source database(s)	Interaction identifier(s)	Confidence value(s)	Expansion method(s)	Biological role(s) interactor A	Biological role(s) interactor B	Experimental role(s) interactor A	Experimental role(s) interactor B	Type(s) interactor A	Type(s) interactor B	Xref(s) interactor A	Xref(s) interactor B	Interaction Xref(s)	Annotation(s) interactor A	Annotation(s) interactor B	Interaction annotation(s)	Host organism(s)	Interaction parameter(s)	Creation date	Update date	Checksum(s) interactor A	Checksum(s) interactor B	Interaction Checksum(s)	Negative	Feature(s) interactor A	Feature(s) interactor B	Stoichiometry(s) interactor A	Stoichiometry(s) interactor B	Identification method participant A	Identification method participant B
uniprotkb:P54645	uniprotkb:Q00613	intact:EBI-7596967|intact:MINT-4566241|ensembl:ENSRNOP00000017626	intact:EBI-719620|uniprotkb:A8MW26|uniprotkb:Q53XT4|uniprotkb:A8K4L0|ensembl:ENSP00000431512|ensembl:ENSP00000493830	psi-mi:aapk1_rat(display_long)|uniprotkb:Prkaa1(gene name)|psi-mi:Prkaa1(display_short)|uniprotkb:Ampk1(gene name synonym)|uniprotkb:Hydroxymethylglutaryl-CoA reductase kinase(gene name synonym)|uniprotkb:Tau-protein kinase PRKAA1(gene name synonym)|uniprotkb:Acetyl-CoA carboxylase kinase(gene name synonym)	psi-mi:hsf1_human(display_long)|uniprotkb:Heat shock transcription factor 1(gene name synonym)|uniprotkb:HSF1(gene name)|psi-mi:HSF1(display_short)|uniprotkb:HSTF1(gene name synonym)	psi-mi:"MI:0007"(anti tag coimmunoprecipitation)	Dai et al. (2015)	imex:IM-23858|pubmed:25425574	taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)")	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0471"(MINT)	intact:EBI-10682831|imex:IM-23858-1	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	rgd:3387|refseq:NP_062015.2|ensembl:ENSRNOG00000012799(gene)|ensembl:ENSRNOT00000017626(transcript)|go:"GO:0003682"(chromatin binding)|go:"GO:0004672"(protein kinase activity)|go:"GO:0004674"(protein serine/threonine kinase activity)|go:"GO:0004679"(AMP-activated protein kinase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005634"(nucleus)|go:"GO:0005737"(cytoplasm)|go:"GO:0005829"(cytosol)|go:"GO:0006006"(glucose metabolic process)|go:"GO:0006468"(protein phosphorylation)|go:"GO:0006633"(fatty acid biosynthetic process)|go:"GO:0006695"(cholesterol biosynthetic process)|go:"GO:0006914"(autophagy)|go:"GO:0008022"(protein C-terminus binding)|go:"GO:0008284"(positive regulation of cell population proliferation)|go:"GO:0008610"(lipid biosynthetic process)|go:"GO:0009411"(response to UV)|go:"GO:0009631"(cold acclimation)|go:"GO:0010332"(response to gamma radiation)|go:"GO:0010468"(regulation of gene expression)|go:"GO:0010508"(positive regulation of autophagy)|go:"GO:0010628"(positive regulation of gene expression)|go:"GO:0010629"(negative regulation of gene expression)|go:"GO:0014823"(response to activity)|go:"GO:0015721"(bile acid and bile salt transport)|go:"GO:0016055"(Wnt signaling pathway)|go:"GO:0016324"(apical plasma membrane)|go:"GO:0016607"(nuclear speck)|go:"GO:0017148"(negative regulation of translation)|go:"GO:0019395"(fatty acid oxidation)|go:"GO:0030424"(axon)|go:"GO:0030425"(dendrite)|go:"GO:0031000"(response to caffeine)|go:"GO:0031588"(nucleotide-activated protein kinase complex)|go:"GO:0031669"(cellular response to nutrient levels)|go:"GO:0032007"(negative regulation of TOR signaling)|go:"GO:0032991"(protein-containing complex)|go:"GO:0033135"(regulation of peptidyl-serine phosphorylation)|go:"GO:0034599"(cellular response to oxidative stress)|go:"GO:0035174"(histone serine kinase activity)|go:"GO:0035556"(intracellular signal transduction)|go:"GO:0035690"|go:"GO:0038183"(bile acid signaling pathway)|go:"GO:0042149"(cellular response to glucose starvation)|go:"GO:0042493"|go:"GO:0042542"(response to hydrogen peroxide)|go:"GO:0042557"(eukaryotic elongation factor-2 kinase activator activity)|go:"GO:0042593"(glucose homeostasis)|go:"GO:0042752"(regulation of circadian rhythm)|go:"GO:0043025"(neuronal cell body)|go:"GO:0043066"(negative regulation of apoptotic process)|go:"GO:0044877"(protein-containing complex binding)|go:"GO:0045722"(positive regulation of gluconeogenesis)|go:"GO:0045821"(positive regulation of glycolytic process)|go:"GO:0046321"(positive regulation of fatty acid oxidation)|go:"GO:0046326"(positive regulation of glucose import)|go:"GO:0046627"(negative regulation of insulin receptor signaling pathway)|go:"GO:0046872"(metal ion binding)|go:"GO:0047322"("[hydroxymethylglutaryl-CoA reductase (NADPH)] kinase activity")|go:"GO:0048511"(rhythmic process)|go:"GO:0048643"(positive regulation of skeletal muscle tissue development)|go:"GO:0050321"(tau-protein kinase activity)|go:"GO:0050405"([acetyl-CoA carboxylase] kinase activity)|go:"GO:0050995"(negative regulation of lipid catabolic process)|go:"GO:0055089"(fatty acid homeostasis)|go:"GO:0060627"(regulation of vesicle-mediated transport)|go:"GO:0061744"(motor behavior)|go:"GO:0061762"(CAMKK-AMPK signaling cascade)|go:"GO:0062028"(regulation of stress granule assembly)|go:"GO:0070050"(neuron cellular homeostasis)|go:"GO:0070301"(cellular response to hydrogen peroxide)|go:"GO:0070507"(regulation of microtubule cytoskeleton organization)|go:"GO:0071277"(cellular response to calcium ion)|go:"GO:0071333"(cellular response to glucose stimulus)|go:"GO:0071361"(cellular response to ethanol)|go:"GO:0071380"(cellular response to prostaglandin E stimulus)|go:"GO:0071417"(cellular response to organonitrogen compound)|go:"GO:0071456"(cellular response to hypoxia)|go:"GO:0097009"(energy homeostasis)|go:"GO:0106310"(protein serine kinase activity)|go:"GO:0106311"|go:"GO:0120188"(regulation of bile acid secretion)|go:"GO:1901563"(response to camptothecin)|go:"GO:1903109"(positive regulation of mitochondrial transcription)|go:"GO:1903829"(positive regulation of protein localization)|go:"GO:1903955"(positive regulation of protein targeting to mitochondrion)|go:"GO:1904428"(negative regulation of tubulin deacetylation)|go:"GO:1904486"(response to 17alpha-ethynylestradiol)|go:"GO:2000758"(positive regulation of peptidyl-lysine acetylation)|interpro:IPR000719(Protein kinase, core)|interpro:IPR008271(Serine/threonine protein kinase, active site)|interpro:IPR011009(Protein kinase-like)|interpro:IPR017441(Protein kinase ATP binding, conserved site)|interpro:IPR028375|interpro:IPR028797|interpro:IPR032270|interpro:IPR039137|mint:P54645|rcsb pdb:2V8Q|rcsb pdb:2V92|rcsb pdb:2V9J|rcsb pdb:2Y8L|rcsb pdb:2Y8Q|rcsb pdb:2YA3|rcsb pdb:4CFH|rcsb pdb:4EAI|rcsb pdb:4EAJ|rcsb pdb:4EAK|rcsb pdb:4EAL|rcsb pdb:4F2L|rcsb pdb:4QFG|rcsb pdb:4QFR|rcsb pdb:4QFS|rcsb pdb:5KQ5|rcsb pdb:5T5T|rcsb pdb:5UFU|rcsb pdb:6E4T|rcsb pdb:6E4U|rcsb pdb:6E4W|reactome:R-RNO-1632852|reactome:R-RNO-380972|reactome:R-RNO-5628897|reactome:R-RNO-6804756|dip:DIP-57168N	refseq:NP_005517.1|go:"GO:0006397"(mRNA processing)|go:"GO:0006952"(defense response)|go:"GO:0007584"(response to nutrient)|go:"GO:0008284"(positive regulation of cell population proliferation)|go:"GO:0009299"(mRNA transcription)|go:"GO:0010628"(positive regulation of gene expression)|go:"GO:0010629"(negative regulation of gene expression)|go:"GO:0010667"(negative regulation of cardiac muscle cell apoptotic process)|go:"GO:0014823"(response to activity)|go:"GO:0016605"(PML body)|go:"GO:0019901"(protein kinase binding)|go:"GO:0031072"(heat shock protein binding)|go:"GO:0031333"(negative regulation of protein-containing complex assembly)|go:"GO:0031490"(chromatin DNA binding)|go:"GO:0033574"(response to testosterone)|go:"GO:0034605"(cellular response to heat)|go:"GO:0034620"(cellular response to unfolded protein)|go:"GO:0034622"|go:"GO:0035865"(cellular response to potassium ion)|go:"GO:0042531"(positive regulation of tyrosine phosphorylation of STAT protein)|go:"GO:0042802"(identical protein binding)|go:"GO:0043280"(positive regulation of cysteine-type endopeptidase activity involved in apoptotic process)|go:"GO:0043565"(sequence-specific DNA binding)|go:"GO:0043621"(protein self-association)|go:"GO:0045651"(positive regulation of macrophage differentiation)|go:"GO:0045931"(positive regulation of mitotic cell cycle)|go:"GO:0045944"(positive regulation of transcription by RNA polymerase II)|go:"GO:0046982"(protein heterodimerization activity)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0051028"(mRNA transport)|go:"GO:0051091"(positive regulation of DNA-binding transcription factor activity)|go:"GO:0051879"(Hsp90 protein binding)|go:"GO:0061408"(positive regulation of transcription from RNA polymerase II promoter in response to heat stress)|go:"GO:0061770"(translation elongation factor binding)|go:"GO:0070301"(cellular response to hydrogen peroxide)|go:"GO:0071222"(cellular response to lipopolysaccharide)|go:"GO:0071276"(cellular response to cadmium ion)|go:"GO:0071280"(cellular response to copper ion)|go:"GO:0071392"(cellular response to estradiol stimulus)|go:"GO:0071480"(cellular response to gamma radiation)|go:"GO:0072738"(cellular response to diamide)|go:"GO:0090084"(negative regulation of inclusion body assembly)|go:"GO:0090261"(positive regulation of inclusion body assembly)|go:"GO:0097165"(nuclear stress granule)|go:"GO:0097431"(mitotic spindle pole)|go:"GO:0097677"(STAT family protein binding)|go:"GO:0098847"(sequence-specific single stranded DNA binding)|go:"GO:0101031"(chaperone complex)|go:"GO:0120162"(positive regulation of cold-induced thermogenesis)|go:"GO:1900034"(regulation of cellular response to heat)|go:"GO:1900365"(positive regulation of mRNA polyadenylation)|go:"GO:1901215"(negative regulation of neuron death)|go:"GO:1902512"(positive regulation of apoptotic DNA fragmentation)|go:"GO:1903936"(cellular response to sodium arsenite)|go:"GO:1904385"(cellular response to angiotensin)|go:"GO:1904528"(positive regulation of microtubule binding)|go:"GO:1904843"(cellular response to nitroglycerin)|go:"GO:1904845"(cellular response to L-glutamine)|go:"GO:1990837"(sequence-specific double-stranded DNA binding)|go:"GO:1990841"(promoter-specific chromatin binding)|go:"GO:1990904"(ribonucleoprotein complex)|go:"GO:1990910"(response to hypobaric hypoxia)|go:"GO:1990911"(response to psychosocial stress)|go:"GO:2001033"(negative regulation of double-strand break repair via nonhomologous end joining)|interpro:IPR000232("Heat shock factor (HSF)-type, DNA-binding")|interpro:IPR010542(Vertebrate heat shock transcription factor)|interpro:IPR027725|interpro:IPR036388|interpro:IPR036390|mint:Q00613|rcsb pdb:2LDU|rcsb pdb:5D5U|rcsb pdb:5D5V|rcsb pdb:5HDG|rcsb pdb:5HDN|reactome:R-HSA-3371453|reactome:R-HSA-3371511|reactome:R-HSA-3371568|reactome:R-HSA-3371571|reactome:R-HSA-9646399|ensembl:ENSG00000185122(gene)|ensembl:ENSG00000284774(gene)|ensembl:ENST00000528838(transcript)|ensembl:ENST00000646252(transcript)|go:"GO:0000122"(negative regulation of transcription by RNA polymerase II)|go:"GO:0000165"(MAPK cascade)|go:"GO:0000776"(kinetochore)|go:"GO:0000785"(chromatin)|go:"GO:0000791"(euchromatin)|go:"GO:0000792"(heterochromatin)|go:"GO:0000976"(transcription cis-regulatory region binding)|go:"GO:0000978"(RNA polymerase II cis-regulatory region sequence-specific DNA binding)|go:"GO:0000981"(DNA-binding transcription factor activity, RNA polymerase II-specific)|go:"GO:0001162"(RNA polymerase II intronic transcription regulatory region sequence-specific DNA binding)|go:"GO:0001227"(DNA-binding transcription repressor activity, RNA polymerase II-specific)|go:"GO:0003677"(DNA binding)|go:"GO:0003700"(DNA-binding transcription factor activity)|go:"GO:0005634"(nucleus)|go:"GO:0005654"(nucleoplasm)|go:"GO:0005737"(cytoplasm)|go:"GO:0005813"(centrosome)|go:"GO:0005829"(cytosol)|go:"GO:0006281"(DNA repair)|go:"GO:0006357"(regulation of transcription by RNA polymerase II)|refseq:XP_016868866.1|dip:DIP-35670N	-	function:Responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. Appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-AMP rises in response to fuel limitation and/or hypoxia. This is a catalytic subunit|comment:mint	-	figure legend:sf2f|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:9606(human-293t)|taxid:9606(Homo sapiens 293 cells transformed with SV40 large T antigen)	-	2015/02/18	2015/02/20	rogid:6ENmHVb5EI3JOKaf/gUMI7w2aqQ10116	rogid:8Kc0CyLMImN2fpNco/AAIbJk+6k9606	intact-crc:7B9EB4FE43FA79E5|rigid:BFRxrm23SMHQrkwBP728A7J7dAM	false	glutathione s tranferase tag:?-?	flag tag:?-?	-	-	psi-mi:"MI:0705"(anti tag western blot)	psi-mi:"MI:0705"(anti tag western blot)
uniprotkb:Q00613	uniprotkb:P02545	intact:EBI-719620|uniprotkb:A8MW26|uniprotkb:Q53XT4|uniprotkb:A8K4L0|ensembl:ENSP00000431512|ensembl:ENSP00000493830	intact:EBI-351935|uniprotkb:B4DI32|uniprotkb:E7EUI9|uniprotkb:P02546|uniprotkb:Q969I8|uniprotkb:Q96JA2|uniprotkb:D6RAQ3|uniprotkb:Q5I6Y4|uniprotkb:Q5I6Y6|uniprotkb:Q6UYC3|uniprotkb:Q5TCJ2|uniprotkb:Q5TCJ3|uniprotkb:D3DVB0|intact:EBI-28988787|ensembl:ENSP00000355292|ensembl:ENSP00000357283|ensembl:ENSP00000502256|ensembl:ENSP00000506771	psi-mi:hsf1_human(display_long)|uniprotkb:Heat shock transcription factor 1(gene name synonym)|uniprotkb:HSF1(gene name)|psi-mi:HSF1(display_short)|uniprotkb:HSTF1(gene name synonym)	psi-mi:lmna_human(display_long)|uniprotkb:LMNA(gene name)|psi-mi:LMNA(display_short)|uniprotkb:LMN1(gene name synonym)	psi-mi:"MI:0029"(cosedimentation through density gradient)	Dai et al. (2015)	imex:IM-23858|pubmed:25425574	taxid:9606(human)|taxid:9606(Homo sapiens)	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0403"(colocalization)	psi-mi:"MI:0471"(MINT)	intact:EBI-10682932|imex:IM-23858-3	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_005517.1|go:"GO:0006397"(mRNA processing)|go:"GO:0006952"(defense response)|go:"GO:0007584"(response to nutrient)|go:"GO:0008284"(positive regulation of cell population proliferation)|go:"GO:0009299"(mRNA transcription)|go:"GO:0010628"(positive regulation of gene expression)|go:"GO:0010629"(negative regulation of gene expression)|go:"GO:0010667"(negative regulation of cardiac muscle cell apoptotic process)|go:"GO:0014823"(response to activity)|go:"GO:0016605"(PML body)|go:"GO:0019901"(protein kinase binding)|go:"GO:0031072"(heat shock protein binding)|go:"GO:0031333"(negative regulation of protein-containing complex assembly)|go:"GO:0031490"(chromatin DNA binding)|go:"GO:0033574"(response to testosterone)|go:"GO:0034605"(cellular response to heat)|go:"GO:0034620"(cellular response to unfolded protein)|go:"GO:0034622"|go:"GO:0035865"(cellular response to potassium ion)|go:"GO:0042531"(positive regulation of tyrosine phosphorylation of STAT protein)|go:"GO:0042802"(identical protein binding)|go:"GO:0043280"(positive regulation of cysteine-type endopeptidase activity involved in apoptotic process)|go:"GO:0043565"(sequence-specific DNA binding)|go:"GO:0043621"(protein self-association)|go:"GO:0045651"(positive regulation of macrophage differentiation)|go:"GO:0045931"(positive regulation of mitotic cell cycle)|go:"GO:0045944"(positive regulation of transcription by RNA polymerase II)|go:"GO:0046982"(protein heterodimerization activity)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0051028"(mRNA transport)|go:"GO:0051091"(positive regulation of DNA-binding transcription factor activity)|go:"GO:0051879"(Hsp90 protein binding)|go:"GO:0061408"(positive regulation of transcription from RNA polymerase II promoter in response to heat stress)|go:"GO:0061770"(translation elongation factor binding)|go:"GO:0070301"(cellular response to hydrogen peroxide)|go:"GO:0071222"(cellular response to lipopolysaccharide)|go:"GO:0071276"(cellular response to cadmium ion)|go:"GO:0071280"(cellular response to copper ion)|go:"GO:0071392"(cellular response to estradiol stimulus)|go:"GO:0071480"(cellular response to gamma radiation)|go:"GO:0072738"(cellular response to diamide)|go:"GO:0090084"(negative regulation of inclusion body assembly)|go:"GO:0090261"(positive regulation of inclusion body assembly)|go:"GO:0097165"(nuclear stress granule)|go:"GO:0097431"(mitotic spindle pole)|go:"GO:0097677"(STAT family protein binding)|go:"GO:0098847"(sequence-specific single stranded DNA binding)|go:"GO:0101031"(chaperone complex)|go:"GO:0120162"(positive regulation of cold-induced thermogenesis)|go:"GO:1900034"(regulation of cellular response to heat)|go:"GO:1900365"(positive regulation of mRNA polyadenylation)|go:"GO:1901215"(negative regulation of neuron death)|go:"GO:1902512"(positive regulation of apoptotic DNA fragmentation)|go:"GO:1903936"(cellular response to sodium arsenite)|go:"GO:1904385"(cellular response to angiotensin)|go:"GO:1904528"(positive regulation of microtubule binding)|go:"GO:1904843"(cellular response to nitroglycerin)|go:"GO:1904845"(cellular response to L-glutamine)|go:"GO:1990837"(sequence-specific double-stranded DNA binding)|go:"GO:1990841"(promoter-specific chromatin binding)|go:"GO:1990904"(ribonucleoprotein complex)|go:"GO:1990910"(response to hypobaric hypoxia)|go:"GO:1990911"(response to psychosocial stress)|go:"GO:2001033"(negative regulation of double-strand break repair via nonhomologous end joining)|interpro:IPR000232("Heat shock factor (HSF)-type, DNA-binding")|interpro:IPR010542(Vertebrate heat shock transcription factor)|interpro:IPR027725|interpro:IPR036388|interpro:IPR036390|mint:Q00613|rcsb pdb:2LDU|rcsb pdb:5D5U|rcsb pdb:5D5V|rcsb pdb:5HDG|rcsb pdb:5HDN|reactome:R-HSA-3371453|reactome:R-HSA-3371511|reactome:R-HSA-3371568|reactome:R-HSA-3371571|reactome:R-HSA-9646399|ensembl:ENSG00000185122(gene)|ensembl:ENSG00000284774(gene)|ensembl:ENST00000528838(transcript)|ensembl:ENST00000646252(transcript)|go:"GO:0000122"(negative regulation of transcription by RNA polymerase II)|go:"GO:0000165"(MAPK cascade)|go:"GO:0000776"(kinetochore)|go:"GO:0000785"(chromatin)|go:"GO:0000791"(euchromatin)|go:"GO:0000792"(heterochromatin)|go:"GO:0000976"(transcription cis-regulatory region binding)|go:"GO:0000978"(RNA polymerase II cis-regulatory region sequence-specific DNA binding)|go:"GO:0000981"(DNA-binding transcription factor activity, RNA polymerase II-specific)|go:"GO:0001162"(RNA polymerase II intronic transcription regulatory region sequence-specific DNA binding)|go:"GO:0001227"(DNA-binding transcription repressor activity, RNA polymerase II-specific)|go:"GO:0003677"(DNA binding)|go:"GO:0003700"(DNA-binding transcription factor activity)|go:"GO:0005634"(nucleus)|go:"GO:0005654"(nucleoplasm)|go:"GO:0005737"(cytoplasm)|go:"GO:0005813"(centrosome)|go:"GO:0005829"(cytosol)|go:"GO:0006281"(DNA repair)|go:"GO:0006357"(regulation of transcription by RNA polymerase II)|refseq:XP_016868866.1|dip:DIP-35670N	refseq:NP_005563.1|refseq:NP_733821.1|refseq:NP_733822.1|refseq:NP_001244303.1|refseq:NP_001269553.1|refseq:NP_001269554.1|refseq:NP_001269555.1|ensembl:ENSG00000160789(gene)|ensembl:ENST00000361308(transcript)|ensembl:ENST00000368300(transcript)|ensembl:ENST00000675939(transcript)|ensembl:ENST00000683032(transcript)|go:"GO:0005198"(structural molecule activity)|go:"GO:0005634"(nucleus)|go:"GO:0005638"(lamin filament)|go:"GO:0005652"(nuclear lamina)|go:"GO:0005654"(nucleoplasm)|go:"GO:0005829"(cytosol)|go:"GO:0005882"(intermediate filament)|go:"GO:0006606"(protein import into nucleus)|go:"GO:0006998"(nuclear envelope organization)|go:"GO:0007517"(muscle organ development)|go:"GO:0008285"(negative regulation of cell population proliferation)|go:"GO:0010628"(positive regulation of gene expression)|go:"GO:0016363"(nuclear matrix)|go:"GO:0016604"(nuclear body)|go:"GO:0016607"(nuclear speck)|go:"GO:0030334"(regulation of cell migration)|go:"GO:0030951"(establishment or maintenance of microtubule cytoskeleton polarity)|go:"GO:0031647"(regulation of protein stability)|go:"GO:0031965"(nuclear membrane)|go:"GO:0032204"(regulation of telomere maintenance)|go:"GO:0034504"(protein localization to nucleus)|go:"GO:0005635"(nuclear envelope)|go:"GO:0034613"(cellular protein localization)|go:"GO:0035861"(site of double-strand break)|go:"GO:0042802"(identical protein binding)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0055015"(ventricular cardiac muscle cell development)|go:"GO:0071456"(cellular response to hypoxia)|go:"GO:0072201"(negative regulation of mesenchymal cell proliferation)|go:"GO:0090201"(negative regulation of release of cytochrome c from mitochondria)|go:"GO:0090343"(positive regulation of cell aging)|go:"GO:1900114"(positive regulation of histone H3-K9 trimethylation)|go:"GO:1900180"(regulation of protein localization to nucleus)|go:"GO:1903243"(negative regulation of cardiac muscle hypertrophy in response to stress)|go:"GO:1990683"(DNA double-strand break attachment to nuclear envelope)|go:"GO:2001237"(negative regulation of extrinsic apoptotic signaling pathway)|interpro:IPR001322(Intermediate filament, C-terminal)|interpro:IPR018039|interpro:IPR036415|interpro:IPR039008|mint:P02545|rcsb pdb:1IFR|rcsb pdb:1IVT|rcsb pdb:1X8Y|rcsb pdb:2XV5|rcsb pdb:2YPT|rcsb pdb:3GEF|rcsb pdb:3V4Q|rcsb pdb:3V4W|rcsb pdb:3V5B|rcsb pdb:6GHD|rcsb pdb:6JLB|rcsb pdb:6RPR|rcsb pdb:6SNZ|rcsb pdb:6YF5|rcsb pdb:6YJD|reactome:R-HSA-1221632|reactome:R-HSA-2980766|reactome:R-HSA-2995383|reactome:R-HSA-352238|reactome:R-HSA-381038|reactome:R-HSA-4419969|reactome:R-HSA-6802952|reactome:R-HSA-8862803|ensembl:ENSP00000503633|ensembl:ENST00000682650|ensembl:ENSP00000506904|ensembl:ENST00000677389|ensembl:ENSP00000506771|ensembl:ENSP00000355292|ensembl:ENST00000368299|ensembl:ENSP00000357282|ensembl:ENSP00000357283|ensembl:ENST00000368301|ensembl:ENSP00000357284|ensembl:ENST00000448611|ensembl:ENSP00000395597|ensembl:ENST00000473598|ensembl:ENSP00000421821|ensembl:ENSP00000502256|ensembl:ENST00000676385|ensembl:ENSP00000502091|dip:DIP-32948N|dip:DIP-58162N	-	-	-	figure legend:f1j|comment:"Next, we investigated  whether metformin affected HSF1 nuclear translocation, a prerequisite  for its DNA binding. As expected, heat shock caused most HSF1  proteins to translocate from the cytoplasm to the nucleus (Fig 1J).  Importantly, metformin impeded this translocation (Fig 1J). Thus,  metformin impairs HSF1–DNA binding at least in part through  blockade of its nuclear translocation."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:9606(human-293t)|taxid:9606(Homo sapiens 293 cells transformed with SV40 large T antigen)	-	2015/02/18	2015/02/20	rogid:8Kc0CyLMImN2fpNco/AAIbJk+6k9606	rogid:OS/QDFLGOD2PElQ6mCpBtk6xh/09606	intact-crc:98B198006464803E|rigid:ELhVxV3YqXtPQjmYP/XPYEfm4CA	false	-	-	-	-	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)
uniprotkb:P02545	uniprotkb:Q00613	intact:EBI-351935|uniprotkb:B4DI32|uniprotkb:E7EUI9|uniprotkb:P02546|uniprotkb:Q969I8|uniprotkb:Q96JA2|uniprotkb:D6RAQ3|uniprotkb:Q5I6Y4|uniprotkb:Q5I6Y6|uniprotkb:Q6UYC3|uniprotkb:Q5TCJ2|uniprotkb:Q5TCJ3|uniprotkb:D3DVB0|intact:EBI-28988787|ensembl:ENSP00000355292|ensembl:ENSP00000357283|ensembl:ENSP00000502256|ensembl:ENSP00000506771	intact:EBI-719620|uniprotkb:A8MW26|uniprotkb:Q53XT4|uniprotkb:A8K4L0|ensembl:ENSP00000431512|ensembl:ENSP00000493830	psi-mi:lmna_human(display_long)|uniprotkb:LMNA(gene name)|psi-mi:LMNA(display_short)|uniprotkb:LMN1(gene name synonym)	psi-mi:hsf1_human(display_long)|uniprotkb:Heat shock transcription factor 1(gene name synonym)|uniprotkb:HSF1(gene name)|psi-mi:HSF1(display_short)|uniprotkb:HSTF1(gene name synonym)	psi-mi:"MI:0029"(cosedimentation through density gradient)	Dai et al. (2015)	imex:IM-23858|pubmed:25425574	taxid:9606(human)|taxid:9606(Homo sapiens)	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0403"(colocalization)	psi-mi:"MI:0471"(MINT)	intact:EBI-10682923|imex:IM-23858-2	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_005563.1|refseq:NP_733821.1|refseq:NP_733822.1|refseq:NP_001244303.1|refseq:NP_001269553.1|refseq:NP_001269554.1|refseq:NP_001269555.1|ensembl:ENSG00000160789(gene)|ensembl:ENST00000361308(transcript)|ensembl:ENST00000368300(transcript)|ensembl:ENST00000675939(transcript)|ensembl:ENST00000683032(transcript)|go:"GO:0005198"(structural molecule activity)|go:"GO:0005634"(nucleus)|go:"GO:0005638"(lamin filament)|go:"GO:0005652"(nuclear lamina)|go:"GO:0005654"(nucleoplasm)|go:"GO:0005829"(cytosol)|go:"GO:0005882"(intermediate filament)|go:"GO:0006606"(protein import into nucleus)|go:"GO:0006998"(nuclear envelope organization)|go:"GO:0007517"(muscle organ development)|go:"GO:0008285"(negative regulation of cell population proliferation)|go:"GO:0010628"(positive regulation of gene expression)|go:"GO:0016363"(nuclear matrix)|go:"GO:0016604"(nuclear body)|go:"GO:0016607"(nuclear speck)|go:"GO:0030334"(regulation of cell migration)|go:"GO:0030951"(establishment or maintenance of microtubule cytoskeleton polarity)|go:"GO:0031647"(regulation of protein stability)|go:"GO:0031965"(nuclear membrane)|go:"GO:0032204"(regulation of telomere maintenance)|go:"GO:0034504"(protein localization to nucleus)|go:"GO:0005635"(nuclear envelope)|go:"GO:0034613"(cellular protein localization)|go:"GO:0035861"(site of double-strand break)|go:"GO:0042802"(identical protein binding)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0055015"(ventricular cardiac muscle cell development)|go:"GO:0071456"(cellular response to hypoxia)|go:"GO:0072201"(negative regulation of mesenchymal cell proliferation)|go:"GO:0090201"(negative regulation of release of cytochrome c from mitochondria)|go:"GO:0090343"(positive regulation of cell aging)|go:"GO:1900114"(positive regulation of histone H3-K9 trimethylation)|go:"GO:1900180"(regulation of protein localization to nucleus)|go:"GO:1903243"(negative regulation of cardiac muscle hypertrophy in response to stress)|go:"GO:1990683"(DNA double-strand break attachment to nuclear envelope)|go:"GO:2001237"(negative regulation of extrinsic apoptotic signaling pathway)|interpro:IPR001322(Intermediate filament, C-terminal)|interpro:IPR018039|interpro:IPR036415|interpro:IPR039008|mint:P02545|rcsb pdb:1IFR|rcsb pdb:1IVT|rcsb pdb:1X8Y|rcsb pdb:2XV5|rcsb pdb:2YPT|rcsb pdb:3GEF|rcsb pdb:3V4Q|rcsb pdb:3V4W|rcsb pdb:3V5B|rcsb pdb:6GHD|rcsb pdb:6JLB|rcsb pdb:6RPR|rcsb pdb:6SNZ|rcsb pdb:6YF5|rcsb pdb:6YJD|reactome:R-HSA-1221632|reactome:R-HSA-2980766|reactome:R-HSA-2995383|reactome:R-HSA-352238|reactome:R-HSA-381038|reactome:R-HSA-4419969|reactome:R-HSA-6802952|reactome:R-HSA-8862803|ensembl:ENSP00000503633|ensembl:ENST00000682650|ensembl:ENSP00000506904|ensembl:ENST00000677389|ensembl:ENSP00000506771|ensembl:ENSP00000355292|ensembl:ENST00000368299|ensembl:ENSP00000357282|ensembl:ENSP00000357283|ensembl:ENST00000368301|ensembl:ENSP00000357284|ensembl:ENST00000448611|ensembl:ENSP00000395597|ensembl:ENST00000473598|ensembl:ENSP00000421821|ensembl:ENSP00000502256|ensembl:ENST00000676385|ensembl:ENSP00000502091|dip:DIP-32948N|dip:DIP-58162N	refseq:NP_005517.1|go:"GO:0006397"(mRNA processing)|go:"GO:0006952"(defense response)|go:"GO:0007584"(response to nutrient)|go:"GO:0008284"(positive regulation of cell population proliferation)|go:"GO:0009299"(mRNA transcription)|go:"GO:0010628"(positive regulation of gene expression)|go:"GO:0010629"(negative regulation of gene expression)|go:"GO:0010667"(negative regulation of cardiac muscle cell apoptotic process)|go:"GO:0014823"(response to activity)|go:"GO:0016605"(PML body)|go:"GO:0019901"(protein kinase binding)|go:"GO:0031072"(heat shock protein binding)|go:"GO:0031333"(negative regulation of protein-containing complex assembly)|go:"GO:0031490"(chromatin DNA binding)|go:"GO:0033574"(response to testosterone)|go:"GO:0034605"(cellular response to heat)|go:"GO:0034620"(cellular response to unfolded protein)|go:"GO:0034622"|go:"GO:0035865"(cellular response to potassium ion)|go:"GO:0042531"(positive regulation of tyrosine phosphorylation of STAT protein)|go:"GO:0042802"(identical protein binding)|go:"GO:0043280"(positive regulation of cysteine-type endopeptidase activity involved in apoptotic process)|go:"GO:0043565"(sequence-specific DNA binding)|go:"GO:0043621"(protein self-association)|go:"GO:0045651"(positive regulation of macrophage differentiation)|go:"GO:0045931"(positive regulation of mitotic cell cycle)|go:"GO:0045944"(positive regulation of transcription by RNA polymerase II)|go:"GO:0046982"(protein heterodimerization activity)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0051028"(mRNA transport)|go:"GO:0051091"(positive regulation of DNA-binding transcription factor activity)|go:"GO:0051879"(Hsp90 protein binding)|go:"GO:0061408"(positive regulation of transcription from RNA polymerase II promoter in response to heat stress)|go:"GO:0061770"(translation elongation factor binding)|go:"GO:0070301"(cellular response to hydrogen peroxide)|go:"GO:0071222"(cellular response to lipopolysaccharide)|go:"GO:0071276"(cellular response to cadmium ion)|go:"GO:0071280"(cellular response to copper ion)|go:"GO:0071392"(cellular response to estradiol stimulus)|go:"GO:0071480"(cellular response to gamma radiation)|go:"GO:0072738"(cellular response to diamide)|go:"GO:0090084"(negative regulation of inclusion body assembly)|go:"GO:0090261"(positive regulation of inclusion body assembly)|go:"GO:0097165"(nuclear stress granule)|go:"GO:0097431"(mitotic spindle pole)|go:"GO:0097677"(STAT family protein binding)|go:"GO:0098847"(sequence-specific single stranded DNA binding)|go:"GO:0101031"(chaperone complex)|go:"GO:0120162"(positive regulation of cold-induced thermogenesis)|go:"GO:1900034"(regulation of cellular response to heat)|go:"GO:1900365"(positive regulation of mRNA polyadenylation)|go:"GO:1901215"(negative regulation of neuron death)|go:"GO:1902512"(positive regulation of apoptotic DNA fragmentation)|go:"GO:1903936"(cellular response to sodium arsenite)|go:"GO:1904385"(cellular response to angiotensin)|go:"GO:1904528"(positive regulation of microtubule binding)|go:"GO:1904843"(cellular response to nitroglycerin)|go:"GO:1904845"(cellular response to L-glutamine)|go:"GO:1990837"(sequence-specific double-stranded DNA binding)|go:"GO:1990841"(promoter-specific chromatin binding)|go:"GO:1990904"(ribonucleoprotein complex)|go:"GO:1990910"(response to hypobaric hypoxia)|go:"GO:1990911"(response to psychosocial stress)|go:"GO:2001033"(negative regulation of double-strand break repair via nonhomologous end joining)|interpro:IPR000232("Heat shock factor (HSF)-type, DNA-binding")|interpro:IPR010542(Vertebrate heat shock transcription factor)|interpro:IPR027725|interpro:IPR036388|interpro:IPR036390|mint:Q00613|rcsb pdb:2LDU|rcsb pdb:5D5U|rcsb pdb:5D5V|rcsb pdb:5HDG|rcsb pdb:5HDN|reactome:R-HSA-3371453|reactome:R-HSA-3371511|reactome:R-HSA-3371568|reactome:R-HSA-3371571|reactome:R-HSA-9646399|ensembl:ENSG00000185122(gene)|ensembl:ENSG00000284774(gene)|ensembl:ENST00000528838(transcript)|ensembl:ENST00000646252(transcript)|go:"GO:0000122"(negative regulation of transcription by RNA polymerase II)|go:"GO:0000165"(MAPK cascade)|go:"GO:0000776"(kinetochore)|go:"GO:0000785"(chromatin)|go:"GO:0000791"(euchromatin)|go:"GO:0000792"(heterochromatin)|go:"GO:0000976"(transcription cis-regulatory region binding)|go:"GO:0000978"(RNA polymerase II cis-regulatory region sequence-specific DNA binding)|go:"GO:0000981"(DNA-binding transcription factor activity, RNA polymerase II-specific)|go:"GO:0001162"(RNA polymerase II intronic transcription regulatory region sequence-specific DNA binding)|go:"GO:0001227"(DNA-binding transcription repressor activity, RNA polymerase II-specific)|go:"GO:0003677"(DNA binding)|go:"GO:0003700"(DNA-binding transcription factor activity)|go:"GO:0005634"(nucleus)|go:"GO:0005654"(nucleoplasm)|go:"GO:0005737"(cytoplasm)|go:"GO:0005813"(centrosome)|go:"GO:0005829"(cytosol)|go:"GO:0006281"(DNA repair)|go:"GO:0006357"(regulation of transcription by RNA polymerase II)|refseq:XP_016868866.1|dip:DIP-35670N	-	-	-	figure legend:f3i f3j|comment:"Despite no impacts on HSF1 expression, AMPKaCA  impaired constitutive nuclear translocation of HSF1WT in HEK293T  cells, indicated by diminished nuclear–cytoplasmic ratio of HSF1  levels (Fig 3I). S121A mutation not only enhanced HSF1 nuclear  translocation under basal conditions but also rendered HSF1 resistant  to the effect of AMPKaCA (Fig 3I). Similarly, while metformin  impaired HSF1WT nuclear translocation, it exerted little effect on  HSF1S121A mutants (Fig 3J). Thus, this defect in nuclear translocation  due to Ser121 phosphorylation at least in part contributes to  reduced HSF1–DNA binding"|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:9606(human-293t)|taxid:9606(Homo sapiens 293 cells transformed with SV40 large T antigen)	-	2015/02/18	2015/02/20	rogid:OS/QDFLGOD2PElQ6mCpBtk6xh/09606	rogid:8Kc0CyLMImN2fpNco/AAIbJk+6k9606	intact-crc:F873D4758D14A52D|rigid:ELhVxV3YqXtPQjmYP/XPYEfm4CA	false	-	flag tag:?-?	-	-	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0705"(anti tag western blot)