#ID(s) interactor A ID(s) interactor B Alt. ID(s) interactor A Alt. ID(s) interactor B Alias(es) interactor A Alias(es) interactor B Interaction detection method(s) Publication 1st author(s) Publication Identifier(s) Taxid interactor A Taxid interactor B Interaction type(s) Source database(s) Interaction identifier(s) Confidence value(s) Expansion method(s) Biological role(s) interactor A Biological role(s) interactor B Experimental role(s) interactor A Experimental role(s) interactor B Type(s) interactor A Type(s) interactor B Xref(s) interactor A Xref(s) interactor B Interaction Xref(s) Annotation(s) interactor A Annotation(s) interactor B Interaction annotation(s) Host organism(s) Interaction parameter(s) Creation date Update date Checksum(s) interactor A Checksum(s) interactor B Interaction Checksum(s) Negative Feature(s) interactor A Feature(s) interactor B Stoichiometry(s) interactor A Stoichiometry(s) interactor B Identification method participant A Identification method participant B uniprotkb:P01872 uniprotkb:P01872 intact:EBI-645833|uniprotkb:P01873 intact:EBI-645833|uniprotkb:P01873 psi-mi:ighm_mouse(display_long)|uniprotkb:Ighm(gene name)|psi-mi:Ighm(display_short)|uniprotkb:Igh-6(gene name synonym) psi-mi:ighm_mouse(display_long)|uniprotkb:Ighm(gene name)|psi-mi:Ighm(display_short)|uniprotkb:Igh-6(gene name synonym) psi-mi:"MI:0813"(proximity ligation assay) Kläsener et al. (2014) imex:IM-23964|pubmed:24963139 taxid:10090(mouse)|taxid:10090(Mus musculus) taxid:10090(mouse)|taxid:10090(Mus musculus) psi-mi:"MI:2364"(proximity) psi-mi:"MI:0469"(IntAct) intact:EBI-10683297|imex:IM-23964-23 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) interpro:IPR007110(Immunoglobulin-like)|interpro:IPR013783(Immunoglobulin-like fold)|interpro:IPR003006(Immunoglobulin/major histocompatibility complex, conserved site)|interpro:IPR003597(Immunoglobulin C1-set)|go:"GO:0002344"(B cell affinity maturation)|go:"GO:0002455"(humoral immune response mediated by circulating immunoglobulin)|go:"GO:0002637"(regulation of immunoglobulin production)|go:"GO:0003823"(antigen binding)|go:"GO:0004888"(transmembrane signaling receptor activity)|go:"GO:0005615"(extracellular space)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0006910"(phagocytosis, recognition)|go:"GO:0006911"(phagocytosis, engulfment)|go:"GO:0006958"(complement activation, classical pathway)|go:"GO:0009897"(external side of plasma membrane)|go:"GO:0009986"(cell surface)|go:"GO:0016020"(membrane)|go:"GO:0016021"(integral component of membrane)|go:"GO:0016064"(immunoglobulin mediated immune response)|go:"GO:0019815"(B cell receptor complex)|go:"GO:0019882"(antigen processing and presentation)|go:"GO:0022604"(regulation of cell morphogenesis)|go:"GO:0030890"(positive regulation of B cell proliferation)|go:"GO:0034987"(immunoglobulin receptor binding)|go:"GO:0042571"(immunoglobulin complex, circulating)|go:"GO:0042742"(defense response to bacterium)|go:"GO:0042802"(identical protein binding)|go:"GO:0043410"(positive regulation of MAPK cascade)|go:"GO:0045022"(early endosome to late endosome transport)|go:"GO:0045087"(innate immune response)|go:"GO:0045807"(positive regulation of endocytosis)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0050731"(positive regulation of peptidyl-tyrosine phosphorylation)|go:"GO:0050778"(positive regulation of immune response)|go:"GO:0050829"(defense response to Gram-negative bacterium)|go:"GO:0050853"(B cell receptor signaling pathway)|go:"GO:0050871"(positive regulation of B cell activation)|rcsb pdb:4BA8|rcsb pdb:4JVU|rcsb pdb:4JVW|reactome:R-MMU-202733|reactome:R-MMU-5690714|reactome:R-MMU-983695|interpro:IPR036179|mint:P01872 interpro:IPR007110(Immunoglobulin-like)|interpro:IPR013783(Immunoglobulin-like fold)|interpro:IPR003006(Immunoglobulin/major histocompatibility complex, conserved site)|interpro:IPR003597(Immunoglobulin C1-set)|go:"GO:0002344"(B cell affinity maturation)|go:"GO:0002455"(humoral immune response mediated by circulating immunoglobulin)|go:"GO:0002637"(regulation of immunoglobulin production)|go:"GO:0003823"(antigen binding)|go:"GO:0004888"(transmembrane signaling receptor activity)|go:"GO:0005615"(extracellular space)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0006910"(phagocytosis, recognition)|go:"GO:0006911"(phagocytosis, engulfment)|go:"GO:0006958"(complement activation, classical pathway)|go:"GO:0009897"(external side of plasma membrane)|go:"GO:0009986"(cell surface)|go:"GO:0016020"(membrane)|go:"GO:0016021"(integral component of membrane)|go:"GO:0016064"(immunoglobulin mediated immune response)|go:"GO:0019815"(B cell receptor complex)|go:"GO:0019882"(antigen processing and presentation)|go:"GO:0022604"(regulation of cell morphogenesis)|go:"GO:0030890"(positive regulation of B cell proliferation)|go:"GO:0034987"(immunoglobulin receptor binding)|go:"GO:0042571"(immunoglobulin complex, circulating)|go:"GO:0042742"(defense response to bacterium)|go:"GO:0042802"(identical protein binding)|go:"GO:0043410"(positive regulation of MAPK cascade)|go:"GO:0045022"(early endosome to late endosome transport)|go:"GO:0045087"(innate immune response)|go:"GO:0045807"(positive regulation of endocytosis)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0050731"(positive regulation of peptidyl-tyrosine phosphorylation)|go:"GO:0050778"(positive regulation of immune response)|go:"GO:0050829"(defense response to Gram-negative bacterium)|go:"GO:0050853"(B cell receptor signaling pathway)|go:"GO:0050871"(positive regulation of B cell activation)|rcsb pdb:4BA8|rcsb pdb:4JVU|rcsb pdb:4JVW|reactome:R-MMU-202733|reactome:R-MMU-5690714|reactome:R-MMU-983695|interpro:IPR036179|mint:P01872 - - - figure legend:1D|comment:"Results did not distinguish between resting and antigen (NIP) stimulated cells."|dataset:Affinomics - Interactions curated for the Affinomics consortium.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:10090(mouse-tko-md-pre-b cells)|taxid:10090("Mouse TKO_MD Pre B Cells:KO for SLP-65, RAG2, CD179b, transfected with NOP specific IgM/IgD BCR") - 2015/02/13 2015/02/24 rogid:4+PnUlhyUfPZS43gGZMtyePhlCw10090 rogid:4+PnUlhyUfPZS43gGZMtyePhlCw10090 intact-crc:FC8AE36A91F2A1EC|rigid:N/cK5ET0O9C0NgHLucUfovmg9nc false - - - 2 psi-mi:"MI:0421"(identification by antibody) psi-mi:"MI:0421"(identification by antibody) uniprotkb:P01872 uniprotkb:P25918 intact:EBI-645833|uniprotkb:P01873 intact:EBI-644463|uniprotkb:Q542B2|ensembl:ENSMUSP00000145803 psi-mi:ighm_mouse(display_long)|uniprotkb:Ighm(gene name)|psi-mi:Ighm(display_short)|uniprotkb:Igh-6(gene name synonym) psi-mi:cd19_mouse(display_long)|uniprotkb:Differentiation antigen CD19(gene name synonym)|uniprotkb:Cd19(gene name)|psi-mi:Cd19(display_short) psi-mi:"MI:0813"(proximity ligation assay) Kläsener et al. (2014) imex:IM-23964|pubmed:24963139 taxid:10090(mouse)|taxid:10090(Mus musculus) taxid:10090(mouse)|taxid:10090(Mus musculus) psi-mi:"MI:2364"(proximity) psi-mi:"MI:0469"(IntAct) intact:EBI-10683852|imex:IM-23964-55 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) interpro:IPR007110(Immunoglobulin-like)|interpro:IPR013783(Immunoglobulin-like fold)|interpro:IPR003006(Immunoglobulin/major histocompatibility complex, conserved site)|interpro:IPR003597(Immunoglobulin C1-set)|go:"GO:0002344"(B cell affinity maturation)|go:"GO:0002455"(humoral immune response mediated by circulating immunoglobulin)|go:"GO:0002637"(regulation of immunoglobulin production)|go:"GO:0003823"(antigen binding)|go:"GO:0004888"(transmembrane signaling receptor activity)|go:"GO:0005615"(extracellular space)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0006910"(phagocytosis, recognition)|go:"GO:0006911"(phagocytosis, engulfment)|go:"GO:0006958"(complement activation, classical pathway)|go:"GO:0009897"(external side of plasma membrane)|go:"GO:0009986"(cell surface)|go:"GO:0016020"(membrane)|go:"GO:0016021"(integral component of membrane)|go:"GO:0016064"(immunoglobulin mediated immune response)|go:"GO:0019815"(B cell receptor complex)|go:"GO:0019882"(antigen processing and presentation)|go:"GO:0022604"(regulation of cell morphogenesis)|go:"GO:0030890"(positive regulation of B cell proliferation)|go:"GO:0034987"(immunoglobulin receptor binding)|go:"GO:0042571"(immunoglobulin complex, circulating)|go:"GO:0042742"(defense response to bacterium)|go:"GO:0042802"(identical protein binding)|go:"GO:0043410"(positive regulation of MAPK cascade)|go:"GO:0045022"(early endosome to late endosome transport)|go:"GO:0045087"(innate immune response)|go:"GO:0045807"(positive regulation of endocytosis)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0050731"(positive regulation of peptidyl-tyrosine phosphorylation)|go:"GO:0050778"(positive regulation of immune response)|go:"GO:0050829"(defense response to Gram-negative bacterium)|go:"GO:0050853"(B cell receptor signaling pathway)|go:"GO:0050871"(positive regulation of B cell activation)|rcsb pdb:4BA8|rcsb pdb:4JVU|rcsb pdb:4JVW|reactome:R-MMU-202733|reactome:R-MMU-5690714|reactome:R-MMU-983695|interpro:IPR036179|mint:P01872 refseq:NP_033974.2|ensembl:ENSMUSG00000030724(gene)|ensembl:ENSMUST00000206325(transcript)|go:"GO:0001923"(B-1 B cell differentiation)|go:"GO:0002322"(B cell proliferation involved in immune response)|go:"GO:0005886"(plasma membrane)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0009897"(external side of plasma membrane)|go:"GO:0016064"(immunoglobulin mediated immune response)|go:"GO:0019724"(B cell mediated immunity)|go:"GO:0032991"(protein-containing complex)|go:"GO:0043552"(positive regulation of phosphatidylinositol 3-kinase activity)|go:"GO:0045121"(membrane raft)|go:"GO:0050851"(antigen receptor-mediated signaling pathway)|go:"GO:0050853"(B cell receptor signaling pathway)|go:"GO:0050855"(regulation of B cell receptor signaling pathway)|go:"GO:0050864"(regulation of B cell activation)|go:"GO:0051281"(positive regulation of release of sequestered calcium ion into cytosol)|interpro:IPR003599(Immunoglobulin subtype)|interpro:IPR007110(Immunoglobulin-like)|interpro:IPR013783(Immunoglobulin-like fold)|interpro:IPR036179|interpro:IPR042341|reactome:R-MMU-1257604|reactome:R-MMU-198933|reactome:R-MMU-6811558|reactome:R-MMU-977606|reactome:R-MMU-983695|refseq:XP_006507346.1 - - - figure legend:7E|comment:CD19 is only found in close association with the activated but not with the resting IgM-BCR.|agonist:Specific antigen- NIP|dataset:Affinomics - Interactions curated for the Affinomics consortium.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:10090(mouse-spleen)|taxid:10090(Mus musculus spleen) - 2015/02/13 2015/02/24 rogid:4+PnUlhyUfPZS43gGZMtyePhlCw10090 rogid:YWZCIrT3MOdFhZd5+gTZhMkF8yc10090 intact-crc:9914E017651C8291|rigid:A/Bc70lm3EejkcIeAaEQImf+Ntg false - - - - psi-mi:"MI:0421"(identification by antibody) psi-mi:"MI:0421"(identification by antibody) uniprotkb:P01882 uniprotkb:P01556 intact:EBI-10683684 intact:EBI-1038383|uniprotkb:Q9JQ02|ensemblbacteria:AAF94613 psi-mi:ighdm_mouse(display_long) psi-mi:chtb_vibch(display_long)|uniprotkb:ctxB(gene name)|psi-mi:ctxB(display_short)|uniprotkb:toxB(gene name synonym)|uniprotkb:VC_1456(locus name)|uniprotkb:Cholera enterotoxin B chain(gene name synonym)|uniprotkb:Cholera enterotoxin gamma chain(gene name synonym)|uniprotkb:Choleragenoid(gene name synonym) psi-mi:"MI:0813"(proximity ligation assay) Kläsener et al. (2014) imex:IM-23964|pubmed:24963139 taxid:10090(mouse)|taxid:10090(Mus musculus) taxid:243277(vibch)|taxid:243277(Vibrio cholerae) psi-mi:"MI:2364"(proximity) psi-mi:"MI:0469"(IntAct) intact:EBI-10683894|imex:IM-23964-35 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) interpro:IPR007110(Immunoglobulin-like)|interpro:IPR013783(Immunoglobulin-like fold)|interpro:IPR003006(Immunoglobulin/major histocompatibility complex, conserved site)|interpro:IPR003597(Immunoglobulin C1-set)|go:"GO:0005886"(plasma membrane)|go:"GO:0016021"(integral component of membrane)|interpro:IPR036179 refseq:NP_231099.1|rcsb pdb:5LZJ|rcsb pdb:6HJD|rcsb pdb:6HMW|rcsb pdb:6HMY|rcsb pdb:6HSV|rcsb pdb:5LZG|rcsb pdb:1CT1|rcsb pdb:1FGB|rcsb pdb:1G8Z|rcsb pdb:1JR0|rcsb pdb:1MD2|rcsb pdb:1RCV|rcsb pdb:1RD9|rcsb pdb:1RDP|rcsb pdb:1RF2|rcsb pdb:1S5B|rcsb pdb:1S5C|rcsb pdb:1S5D|rcsb pdb:1S5E|rcsb pdb:1S5F|rcsb pdb:1XTC|rcsb pdb:2CHB|rcsb pdb:3CHB|rcsb pdb:3EFX|rcsb pdb:1CHQ|rcsb pdb:5ELC|rcsb pdb:5ELE|rcsb pdb:5ELF|ensemblbacteria:AAF94613(transcript)|ensemblbacteria:VC_1456(gene)|go:"GO:0005534"(galactose binding)|go:"GO:0005576"(extracellular region)|go:"GO:0006471"(protein ADP-ribosylation)|go:"GO:0016020"(membrane)|go:"GO:0020002"(host cell plasma membrane)|go:"GO:0031640"(killing of cells of another organism)|go:"GO:0042531"(positive regulation of tyrosine phosphorylation of STAT protein)|go:"GO:0042597"(periplasmic space)|go:"GO:0046812"(host cell surface binding)|go:"GO:0090729"(toxin activity)|go:"GO:1902494"(catalytic complex)|interpro:IPR001835(Heat-labile enterotoxin, B chain)|interpro:IPR008992(Enterotoxin, bacterial)|rcsb pdb:1CHP|refseq:WP_000593522.1|mint:P01556|dip:DIP-6256N - - - figure legend:7G-2|comment:"On resting B cells, GM1 gangliosides (CTB) are found in close proximity to the IgD-BCR; IgM-BCR gains access to GM1 gangliosides (CTB) only upon B cell activation."|antagonist:"specific antigen-NIP, pervanadate (Perv) and Lat-A - activators of the BCR."|dataset:Affinomics - Interactions curated for the Affinomics consortium.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:10090(mouse-spleen)|taxid:10090(Mus musculus spleen) - 2015/02/13 2015/03/05 rogid:WPb0XrDhxuYDQ0jFtnq2QDd6u1c10090 rogid:i5ZR7CiY2sBXsmmn1mNNPRMV9JU243277 intact-crc:B4A5A5862DFCAC3B|rigid:SI4b3wM7KkUvjLj/rt2Km6WtxiI false - - - - psi-mi:"MI:0421"(identification by antibody) psi-mi:"MI:0421"(identification by antibody) uniprotkb:P19437 uniprotkb:P01882 intact:EBI-10683749|ensembl:ENSMUSP00000126422 intact:EBI-10683684 psi-mi:cd20_mouse(display_long)|uniprotkb:Ms4a1(gene name)|psi-mi:Ms4a1(display_short)|uniprotkb:Cd20(gene name synonym)|uniprotkb:Ly-44(gene name synonym)|uniprotkb:Ms4a2(gene name synonym)|uniprotkb:B-cell differentiation antigen Ly-44(gene name synonym)|uniprotkb:Lymphocyte antigen 44(gene name synonym)|uniprotkb:Membrane-spanning 4-domains subfamily A member 1(gene name synonym) psi-mi:ighdm_mouse(display_long) psi-mi:"MI:0813"(proximity ligation assay) Kläsener et al. (2014) imex:IM-23964|pubmed:24963139 taxid:10090(mouse)|taxid:10090(Mus musculus) taxid:10090(mouse)|taxid:10090(Mus musculus) psi-mi:"MI:2364"(proximity) psi-mi:"MI:0469"(IntAct) intact:EBI-10683870|imex:IM-23964-31 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) refseq:NP_031667.1|ensembl:ENSMUSG00000024673(gene)|ensembl:ENSMUST00000169159(transcript)|go:"GO:0002115"(store-operated calcium entry)|go:"GO:0005154"(epidermal growth factor receptor binding)|go:"GO:0005654"(nucleoplasm)|go:"GO:0005886"(plasma membrane)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0009617"(response to bacterium)|go:"GO:0009897"(external side of plasma membrane)|go:"GO:0009986"(cell surface)|go:"GO:0019865"(immunoglobulin binding)|go:"GO:0030183"(B cell differentiation)|go:"GO:0042113"(B cell activation)|go:"GO:0042802"(identical protein binding)|go:"GO:0044853"(plasma membrane raft)|go:"GO:0050853"(B cell receptor signaling pathway)|go:"GO:0051262"(protein tetramerization)|go:"GO:1902656"(calcium ion import into cytosol)|interpro:IPR007237(CD20/IgE Fc receptor beta subunit)|interpro:IPR030417|interpro:IPR030418 interpro:IPR007110(Immunoglobulin-like)|interpro:IPR013783(Immunoglobulin-like fold)|interpro:IPR003006(Immunoglobulin/major histocompatibility complex, conserved site)|interpro:IPR003597(Immunoglobulin C1-set)|go:"GO:0005886"(plasma membrane)|go:"GO:0016021"(integral component of membrane)|interpro:IPR036179 - - - figure legend:7F-2|comment:CD19 and 20 are found together with the resting IgD-BCR and dissociate from this receptor upon BCR activation|antagonist:Specific antigen- NIP|dataset:Affinomics - Interactions curated for the Affinomics consortium.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:10090(mouse-spleen)|taxid:10090(Mus musculus spleen) - 2015/02/13 2015/02/24 rogid:GPiXJuTEbnfqoUhQ0apzTyBcbzg10090 rogid:WPb0XrDhxuYDQ0jFtnq2QDd6u1c10090 intact-crc:20020F010826E35E|rigid:+ZdVbKS/sTYEGBe3RKOlZyer88E false - - - - psi-mi:"MI:0421"(identification by antibody) psi-mi:"MI:0421"(identification by antibody) uniprotkb:P01882 uniprotkb:P25918 intact:EBI-10683684 intact:EBI-644463|uniprotkb:Q542B2|ensembl:ENSMUSP00000145803 psi-mi:ighdm_mouse(display_long) psi-mi:cd19_mouse(display_long)|uniprotkb:Differentiation antigen CD19(gene name synonym)|uniprotkb:Cd19(gene name)|psi-mi:Cd19(display_short) psi-mi:"MI:0813"(proximity ligation assay) Kläsener et al. (2014) imex:IM-23964|pubmed:24963139 taxid:10090(mouse)|taxid:10090(Mus musculus) taxid:10090(mouse)|taxid:10090(Mus musculus) psi-mi:"MI:2364"(proximity) psi-mi:"MI:0469"(IntAct) intact:EBI-10683864|imex:IM-23964-29 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) interpro:IPR007110(Immunoglobulin-like)|interpro:IPR013783(Immunoglobulin-like fold)|interpro:IPR003006(Immunoglobulin/major histocompatibility complex, conserved site)|interpro:IPR003597(Immunoglobulin C1-set)|go:"GO:0005886"(plasma membrane)|go:"GO:0016021"(integral component of membrane)|interpro:IPR036179 refseq:NP_033974.2|ensembl:ENSMUSG00000030724(gene)|ensembl:ENSMUST00000206325(transcript)|go:"GO:0001923"(B-1 B cell differentiation)|go:"GO:0002322"(B cell proliferation involved in immune response)|go:"GO:0005886"(plasma membrane)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0009897"(external side of plasma membrane)|go:"GO:0016064"(immunoglobulin mediated immune response)|go:"GO:0019724"(B cell mediated immunity)|go:"GO:0032991"(protein-containing complex)|go:"GO:0043552"(positive regulation of phosphatidylinositol 3-kinase activity)|go:"GO:0045121"(membrane raft)|go:"GO:0050851"(antigen receptor-mediated signaling pathway)|go:"GO:0050853"(B cell receptor signaling pathway)|go:"GO:0050855"(regulation of B cell receptor signaling pathway)|go:"GO:0050864"(regulation of B cell activation)|go:"GO:0051281"(positive regulation of release of sequestered calcium ion into cytosol)|interpro:IPR003599(Immunoglobulin subtype)|interpro:IPR007110(Immunoglobulin-like)|interpro:IPR013783(Immunoglobulin-like fold)|interpro:IPR036179|interpro:IPR042341|reactome:R-MMU-1257604|reactome:R-MMU-198933|reactome:R-MMU-6811558|reactome:R-MMU-977606|reactome:R-MMU-983695|refseq:XP_006507346.1 - - - figure legend:7F|comment:CD19 and 20 are found together with the resting IgD-BCR and dissociate from this receptor upon BCR activation|antagonist:Specific antigen- NIP|dataset:Affinomics - Interactions curated for the Affinomics consortium.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:10090(mouse-spleen)|taxid:10090(Mus musculus spleen) - 2015/02/13 2015/02/24 rogid:WPb0XrDhxuYDQ0jFtnq2QDd6u1c10090 rogid:YWZCIrT3MOdFhZd5+gTZhMkF8yc10090 intact-crc:158D508A840F7A05|rigid:rUMykdLzTsMp4bvtu6Aylx21hIQ false - - - - psi-mi:"MI:0421"(identification by antibody) psi-mi:"MI:0421"(identification by antibody) uniprotkb:P01872 uniprotkb:P01872 intact:EBI-645833|uniprotkb:P01873 intact:EBI-645833|uniprotkb:P01873 psi-mi:ighm_mouse(display_long)|uniprotkb:Ighm(gene name)|psi-mi:Ighm(display_short)|uniprotkb:Igh-6(gene name synonym) psi-mi:ighm_mouse(display_long)|uniprotkb:Ighm(gene name)|psi-mi:Ighm(display_short)|uniprotkb:Igh-6(gene name synonym) psi-mi:"MI:0813"(proximity ligation assay) Kläsener et al. (2014) imex:IM-23964|pubmed:24963139 taxid:10090(mouse)|taxid:10090(Mus musculus) taxid:10090(mouse)|taxid:10090(Mus musculus) psi-mi:"MI:2364"(proximity) psi-mi:"MI:0469"(IntAct) intact:EBI-10683487|imex:IM-23964-43 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) interpro:IPR007110(Immunoglobulin-like)|interpro:IPR013783(Immunoglobulin-like fold)|interpro:IPR003006(Immunoglobulin/major histocompatibility complex, conserved site)|interpro:IPR003597(Immunoglobulin C1-set)|go:"GO:0002344"(B cell affinity maturation)|go:"GO:0002455"(humoral immune response mediated by circulating immunoglobulin)|go:"GO:0002637"(regulation of immunoglobulin production)|go:"GO:0003823"(antigen binding)|go:"GO:0004888"(transmembrane signaling receptor activity)|go:"GO:0005615"(extracellular space)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0006910"(phagocytosis, recognition)|go:"GO:0006911"(phagocytosis, engulfment)|go:"GO:0006958"(complement activation, classical pathway)|go:"GO:0009897"(external side of plasma membrane)|go:"GO:0009986"(cell surface)|go:"GO:0016020"(membrane)|go:"GO:0016021"(integral component of membrane)|go:"GO:0016064"(immunoglobulin mediated immune response)|go:"GO:0019815"(B cell receptor complex)|go:"GO:0019882"(antigen processing and presentation)|go:"GO:0022604"(regulation of cell morphogenesis)|go:"GO:0030890"(positive regulation of B cell proliferation)|go:"GO:0034987"(immunoglobulin receptor binding)|go:"GO:0042571"(immunoglobulin complex, circulating)|go:"GO:0042742"(defense response to bacterium)|go:"GO:0042802"(identical protein binding)|go:"GO:0043410"(positive regulation of MAPK cascade)|go:"GO:0045022"(early endosome to late endosome transport)|go:"GO:0045087"(innate immune response)|go:"GO:0045807"(positive regulation of endocytosis)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0050731"(positive regulation of peptidyl-tyrosine phosphorylation)|go:"GO:0050778"(positive regulation of immune response)|go:"GO:0050829"(defense response to Gram-negative bacterium)|go:"GO:0050853"(B cell receptor signaling pathway)|go:"GO:0050871"(positive regulation of B cell activation)|rcsb pdb:4BA8|rcsb pdb:4JVU|rcsb pdb:4JVW|reactome:R-MMU-202733|reactome:R-MMU-5690714|reactome:R-MMU-983695|interpro:IPR036179|mint:P01872 interpro:IPR007110(Immunoglobulin-like)|interpro:IPR013783(Immunoglobulin-like fold)|interpro:IPR003006(Immunoglobulin/major histocompatibility complex, conserved site)|interpro:IPR003597(Immunoglobulin C1-set)|go:"GO:0002344"(B cell affinity maturation)|go:"GO:0002455"(humoral immune response mediated by circulating immunoglobulin)|go:"GO:0002637"(regulation of immunoglobulin production)|go:"GO:0003823"(antigen binding)|go:"GO:0004888"(transmembrane signaling receptor activity)|go:"GO:0005615"(extracellular space)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0006910"(phagocytosis, recognition)|go:"GO:0006911"(phagocytosis, engulfment)|go:"GO:0006958"(complement activation, classical pathway)|go:"GO:0009897"(external side of plasma membrane)|go:"GO:0009986"(cell surface)|go:"GO:0016020"(membrane)|go:"GO:0016021"(integral component of membrane)|go:"GO:0016064"(immunoglobulin mediated immune response)|go:"GO:0019815"(B cell receptor complex)|go:"GO:0019882"(antigen processing and presentation)|go:"GO:0022604"(regulation of cell morphogenesis)|go:"GO:0030890"(positive regulation of B cell proliferation)|go:"GO:0034987"(immunoglobulin receptor binding)|go:"GO:0042571"(immunoglobulin complex, circulating)|go:"GO:0042742"(defense response to bacterium)|go:"GO:0042802"(identical protein binding)|go:"GO:0043410"(positive regulation of MAPK cascade)|go:"GO:0045022"(early endosome to late endosome transport)|go:"GO:0045087"(innate immune response)|go:"GO:0045807"(positive regulation of endocytosis)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0050731"(positive regulation of peptidyl-tyrosine phosphorylation)|go:"GO:0050778"(positive regulation of immune response)|go:"GO:0050829"(defense response to Gram-negative bacterium)|go:"GO:0050853"(B cell receptor signaling pathway)|go:"GO:0050871"(positive regulation of B cell activation)|rcsb pdb:4BA8|rcsb pdb:4JVU|rcsb pdb:4JVW|reactome:R-MMU-202733|reactome:R-MMU-5690714|reactome:R-MMU-983695|interpro:IPR036179|mint:P01872 - - - figure legend:4A/C, 5A/B/C, Supp 5|comment:The Fab-PLA signal is lost upon exposure of B cells to antigen, or in presence of BCR-activating drugs.|antagonist:"specific antigen-NIP, pervanadate (Perv) and Lat-A - activators of the BCR."|comment:When cells were pretreated with R406 - a Syk inhibitor, Lat-A activation did not occur and no loss of signal was seen. Pre-treatment with Src-family kinase inhibitor PP2 only delays the loss of signal.|agonist:R406 - a Syk inhibitor, stops the loss of signal after the specific antigen-NIP stimulation.|dataset:Affinomics - Interactions curated for the Affinomics consortium.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:10090(mouse-spleen)|taxid:10090(Mus musculus spleen) - 2015/02/13 2015/02/24 rogid:4+PnUlhyUfPZS43gGZMtyePhlCw10090 rogid:4+PnUlhyUfPZS43gGZMtyePhlCw10090 intact-crc:F5D3319E0C0B4193|rigid:N/cK5ET0O9C0NgHLucUfovmg9nc false - - - - psi-mi:"MI:0421"(identification by antibody) psi-mi:"MI:0421"(identification by antibody) uniprotkb:P01872 uniprotkb:P01556 intact:EBI-645833|uniprotkb:P01873 intact:EBI-1038383|uniprotkb:Q9JQ02|ensemblbacteria:AAF94613 psi-mi:ighm_mouse(display_long)|uniprotkb:Ighm(gene name)|psi-mi:Ighm(display_short)|uniprotkb:Igh-6(gene name synonym) psi-mi:chtb_vibch(display_long)|uniprotkb:ctxB(gene name)|psi-mi:ctxB(display_short)|uniprotkb:toxB(gene name synonym)|uniprotkb:VC_1456(locus name)|uniprotkb:Cholera enterotoxin B chain(gene name synonym)|uniprotkb:Cholera enterotoxin gamma chain(gene name synonym)|uniprotkb:Choleragenoid(gene name synonym) psi-mi:"MI:0813"(proximity ligation assay) Kläsener et al. (2014) imex:IM-23964|pubmed:24963139 taxid:10090(mouse)|taxid:10090(Mus musculus) taxid:243277(vibch)|taxid:243277(Vibrio cholerae) psi-mi:"MI:2364"(proximity) psi-mi:"MI:0469"(IntAct) intact:EBI-10683888|imex:IM-23964-33 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) interpro:IPR007110(Immunoglobulin-like)|interpro:IPR013783(Immunoglobulin-like fold)|interpro:IPR003006(Immunoglobulin/major histocompatibility complex, conserved site)|interpro:IPR003597(Immunoglobulin C1-set)|go:"GO:0002344"(B cell affinity maturation)|go:"GO:0002455"(humoral immune response mediated by circulating immunoglobulin)|go:"GO:0002637"(regulation of immunoglobulin production)|go:"GO:0003823"(antigen binding)|go:"GO:0004888"(transmembrane signaling receptor activity)|go:"GO:0005615"(extracellular space)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0006910"(phagocytosis, recognition)|go:"GO:0006911"(phagocytosis, engulfment)|go:"GO:0006958"(complement activation, classical pathway)|go:"GO:0009897"(external side of plasma membrane)|go:"GO:0009986"(cell surface)|go:"GO:0016020"(membrane)|go:"GO:0016021"(integral component of membrane)|go:"GO:0016064"(immunoglobulin mediated immune response)|go:"GO:0019815"(B cell receptor complex)|go:"GO:0019882"(antigen processing and presentation)|go:"GO:0022604"(regulation of cell morphogenesis)|go:"GO:0030890"(positive regulation of B cell proliferation)|go:"GO:0034987"(immunoglobulin receptor binding)|go:"GO:0042571"(immunoglobulin complex, circulating)|go:"GO:0042742"(defense response to bacterium)|go:"GO:0042802"(identical protein binding)|go:"GO:0043410"(positive regulation of MAPK cascade)|go:"GO:0045022"(early endosome to late endosome transport)|go:"GO:0045087"(innate immune response)|go:"GO:0045807"(positive regulation of endocytosis)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0050731"(positive regulation of peptidyl-tyrosine phosphorylation)|go:"GO:0050778"(positive regulation of immune response)|go:"GO:0050829"(defense response to Gram-negative bacterium)|go:"GO:0050853"(B cell receptor signaling pathway)|go:"GO:0050871"(positive regulation of B cell activation)|rcsb pdb:4BA8|rcsb pdb:4JVU|rcsb pdb:4JVW|reactome:R-MMU-202733|reactome:R-MMU-5690714|reactome:R-MMU-983695|interpro:IPR036179|mint:P01872 refseq:NP_231099.1|rcsb pdb:5LZJ|rcsb pdb:6HJD|rcsb pdb:6HMW|rcsb pdb:6HMY|rcsb pdb:6HSV|rcsb pdb:5LZG|rcsb pdb:1CT1|rcsb pdb:1FGB|rcsb pdb:1G8Z|rcsb pdb:1JR0|rcsb pdb:1MD2|rcsb pdb:1RCV|rcsb pdb:1RD9|rcsb pdb:1RDP|rcsb pdb:1RF2|rcsb pdb:1S5B|rcsb pdb:1S5C|rcsb pdb:1S5D|rcsb pdb:1S5E|rcsb pdb:1S5F|rcsb pdb:1XTC|rcsb pdb:2CHB|rcsb pdb:3CHB|rcsb pdb:3EFX|rcsb pdb:1CHQ|rcsb pdb:5ELC|rcsb pdb:5ELE|rcsb pdb:5ELF|ensemblbacteria:AAF94613(transcript)|ensemblbacteria:VC_1456(gene)|go:"GO:0005534"(galactose binding)|go:"GO:0005576"(extracellular region)|go:"GO:0006471"(protein ADP-ribosylation)|go:"GO:0016020"(membrane)|go:"GO:0020002"(host cell plasma membrane)|go:"GO:0031640"(killing of cells of another organism)|go:"GO:0042531"(positive regulation of tyrosine phosphorylation of STAT protein)|go:"GO:0042597"(periplasmic space)|go:"GO:0046812"(host cell surface binding)|go:"GO:0090729"(toxin activity)|go:"GO:1902494"(catalytic complex)|interpro:IPR001835(Heat-labile enterotoxin, B chain)|interpro:IPR008992(Enterotoxin, bacterial)|rcsb pdb:1CHP|refseq:WP_000593522.1|mint:P01556|dip:DIP-6256N - - - figure legend:7G|comment:"IgM-BCR gains access to GM1 gangliosides (CTB) only upon B cell activation."|agonist:"specific antigen-NIP, pervanadate (Perv) and Lat-A - activators of the BCR."|dataset:Affinomics - Interactions curated for the Affinomics consortium.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:10090(mouse-spleen)|taxid:10090(Mus musculus spleen) - 2015/02/13 2015/02/24 rogid:4+PnUlhyUfPZS43gGZMtyePhlCw10090 rogid:i5ZR7CiY2sBXsmmn1mNNPRMV9JU243277 intact-crc:2AD1F2642EFF9940|rigid:inprkMp1Wi6xvWnCptyFzXQHMgM false - - - - psi-mi:"MI:0421"(identification by antibody) psi-mi:"MI:0421"(identification by antibody) uniprotkb:P01872 uniprotkb:P19437 intact:EBI-645833|uniprotkb:P01873 intact:EBI-10683749|ensembl:ENSMUSP00000126422 psi-mi:ighm_mouse(display_long)|uniprotkb:Ighm(gene name)|psi-mi:Ighm(display_short)|uniprotkb:Igh-6(gene name synonym) psi-mi:cd20_mouse(display_long)|uniprotkb:Ms4a1(gene name)|psi-mi:Ms4a1(display_short)|uniprotkb:Cd20(gene name synonym)|uniprotkb:Ly-44(gene name synonym)|uniprotkb:Ms4a2(gene name synonym)|uniprotkb:B-cell differentiation antigen Ly-44(gene name synonym)|uniprotkb:Lymphocyte antigen 44(gene name synonym)|uniprotkb:Membrane-spanning 4-domains subfamily A member 1(gene name synonym) psi-mi:"MI:0813"(proximity ligation assay) Kläsener et al. (2014) imex:IM-23964|pubmed:24963139 taxid:10090(mouse)|taxid:10090(Mus musculus) taxid:10090(mouse)|taxid:10090(Mus musculus) psi-mi:"MI:2364"(proximity) psi-mi:"MI:0469"(IntAct) intact:EBI-10683858|imex:IM-23964-57 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) interpro:IPR007110(Immunoglobulin-like)|interpro:IPR013783(Immunoglobulin-like fold)|interpro:IPR003006(Immunoglobulin/major histocompatibility complex, conserved site)|interpro:IPR003597(Immunoglobulin C1-set)|go:"GO:0002344"(B cell affinity maturation)|go:"GO:0002455"(humoral immune response mediated by circulating immunoglobulin)|go:"GO:0002637"(regulation of immunoglobulin production)|go:"GO:0003823"(antigen binding)|go:"GO:0004888"(transmembrane signaling receptor activity)|go:"GO:0005615"(extracellular space)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0006910"(phagocytosis, recognition)|go:"GO:0006911"(phagocytosis, engulfment)|go:"GO:0006958"(complement activation, classical pathway)|go:"GO:0009897"(external side of plasma membrane)|go:"GO:0009986"(cell surface)|go:"GO:0016020"(membrane)|go:"GO:0016021"(integral component of membrane)|go:"GO:0016064"(immunoglobulin mediated immune response)|go:"GO:0019815"(B cell receptor complex)|go:"GO:0019882"(antigen processing and presentation)|go:"GO:0022604"(regulation of cell morphogenesis)|go:"GO:0030890"(positive regulation of B cell proliferation)|go:"GO:0034987"(immunoglobulin receptor binding)|go:"GO:0042571"(immunoglobulin complex, circulating)|go:"GO:0042742"(defense response to bacterium)|go:"GO:0042802"(identical protein binding)|go:"GO:0043410"(positive regulation of MAPK cascade)|go:"GO:0045022"(early endosome to late endosome transport)|go:"GO:0045087"(innate immune response)|go:"GO:0045807"(positive regulation of endocytosis)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0050731"(positive regulation of peptidyl-tyrosine phosphorylation)|go:"GO:0050778"(positive regulation of immune response)|go:"GO:0050829"(defense response to Gram-negative bacterium)|go:"GO:0050853"(B cell receptor signaling pathway)|go:"GO:0050871"(positive regulation of B cell activation)|rcsb pdb:4BA8|rcsb pdb:4JVU|rcsb pdb:4JVW|reactome:R-MMU-202733|reactome:R-MMU-5690714|reactome:R-MMU-983695|interpro:IPR036179|mint:P01872 refseq:NP_031667.1|ensembl:ENSMUSG00000024673(gene)|ensembl:ENSMUST00000169159(transcript)|go:"GO:0002115"(store-operated calcium entry)|go:"GO:0005154"(epidermal growth factor receptor binding)|go:"GO:0005654"(nucleoplasm)|go:"GO:0005886"(plasma membrane)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0009617"(response to bacterium)|go:"GO:0009897"(external side of plasma membrane)|go:"GO:0009986"(cell surface)|go:"GO:0019865"(immunoglobulin binding)|go:"GO:0030183"(B cell differentiation)|go:"GO:0042113"(B cell activation)|go:"GO:0042802"(identical protein binding)|go:"GO:0044853"(plasma membrane raft)|go:"GO:0050853"(B cell receptor signaling pathway)|go:"GO:0051262"(protein tetramerization)|go:"GO:1902656"(calcium ion import into cytosol)|interpro:IPR007237(CD20/IgE Fc receptor beta subunit)|interpro:IPR030417|interpro:IPR030418 - - - figure legend:7E-2|comment:CD20 is only found in close association with the activated but not with the resting IgM-BCR.|agonist:Specific antigen- NIP|dataset:Affinomics - Interactions curated for the Affinomics consortium.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:10090(mouse-spleen)|taxid:10090(Mus musculus spleen) - 2015/02/13 2015/02/24 rogid:4+PnUlhyUfPZS43gGZMtyePhlCw10090 rogid:GPiXJuTEbnfqoUhQ0apzTyBcbzg10090 intact-crc:28BEDAA68126E63A|rigid:eOiDaeqdonr2Q7LU76dmHoQK5I0 false - - - - psi-mi:"MI:0421"(identification by antibody) psi-mi:"MI:0421"(identification by antibody) uniprotkb:P48025 uniprotkb:P11911 intact:EBI-300116|ensembl:ENSMUSP00000060828|ensembl:ENSMUSP00000113852 intact:EBI-10683585|uniprotkb:Q6GTY0|ensembl:ENSMUSP00000003469 psi-mi:ksyk_mouse(display_long)|uniprotkb:Syk(gene name)|psi-mi:Syk(display_short)|uniprotkb:Spleen tyrosine kinase(gene name synonym)|uniprotkb:Sykb(gene name synonym)|uniprotkb:ptk72(gene name synonym) psi-mi:cd79a_mouse(display_long)|uniprotkb:Cd79a(gene name)|psi-mi:Cd79a(display_short)|uniprotkb:Iga(gene name synonym)|uniprotkb:Mb-1(gene name synonym)|uniprotkb:Ig-alpha(gene name synonym)|uniprotkb:MB-1 membrane glycoprotein(gene name synonym)|uniprotkb:Membrane-bound immunoglobulin-associated protein(gene name synonym)|uniprotkb:Surface IgM-associated protein(gene name synonym) psi-mi:"MI:0813"(proximity ligation assay) Kläsener et al. (2014) imex:IM-23964|pubmed:24963139 taxid:10090(mouse)|taxid:10090(Mus musculus) taxid:10090(mouse)|taxid:10090(Mus musculus) psi-mi:"MI:2364"(proximity) psi-mi:"MI:0469"(IntAct) intact:EBI-10683579|imex:IM-23964-47 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) ensembl:ENSMUST00000055087(transcript)|ensembl:ENSMUST00000120135(transcript)|go:"GO:0001525"(angiogenesis)|go:"GO:0001775"(cell activation)|go:"GO:0001784"(phosphotyrosine residue binding)|go:"GO:0001819"(positive regulation of cytokine production)|go:"GO:0001820"(serotonin secretion)|go:"GO:0001945"(lymph vessel development)|go:"GO:0002092"(positive regulation of receptor internalization)|go:"GO:0002223"(stimulatory C-type lectin receptor signaling pathway)|go:"GO:0002250"(adaptive immune response)|go:"GO:0002281"(macrophage activation involved in immune response)|go:"GO:0002283"(neutrophil activation involved in immune response)|go:"GO:0002366"(leukocyte activation involved in immune response)|go:"GO:0002554"(serotonin secretion by platelet)|go:"GO:0004672"(protein kinase activity)|go:"GO:0004674"(protein serine/threonine kinase activity)|go:"GO:0004713"(protein tyrosine kinase activity)|go:"GO:0004714"(transmembrane receptor protein tyrosine kinase activity)|go:"GO:0004715"(non-membrane spanning protein tyrosine kinase activity)|go:"GO:0005102"(signaling receptor binding)|go:"GO:0005178"(integrin binding)|go:"GO:0005524"(ATP binding)|go:"GO:0005634"(nucleus)|go:"GO:0005737"(cytoplasm)|ensembl:ENSMUSG00000021457(gene)|go:"GO:0005829"(cytosol)|go:"GO:0006468"(protein phosphorylation)|go:"GO:0006606"(protein import into nucleus)|go:"GO:0007159"(leukocyte cell-cell adhesion)|go:"GO:0007166"(cell surface receptor signaling pathway)|go:"GO:0007167"(enzyme linked receptor protein signaling pathway)|go:"GO:0007169"(transmembrane receptor protein tyrosine kinase signaling pathway)|go:"GO:0007186"(G protein-coupled receptor signaling pathway)|go:"GO:0007229"(integrin-mediated signaling pathway)|go:"GO:0010543"(regulation of platelet activation)|go:"GO:0019815"(B cell receptor complex)|go:"GO:0019901"(protein kinase binding)|go:"GO:0019902"(phosphatase binding)|go:"GO:0019904"(protein domain specific binding)|go:"GO:0030154"(cell differentiation)|go:"GO:0030593"(neutrophil chemotaxis)|go:"GO:0031234"(extrinsic component of cytoplasmic side of plasma membrane)|go:"GO:0031334"(positive regulation of protein-containing complex assembly)|go:"GO:0031623"(receptor internalization)|go:"GO:0031625"(ubiquitin protein ligase binding)|go:"GO:0032009"(early phagosome)|go:"GO:0032481"(positive regulation of type I interferon production)|go:"GO:0032725"(positive regulation of granulocyte macrophage colony-stimulating factor production)|go:"GO:0032733"(positive regulation of interleukin-10 production)|go:"GO:0032735"(positive regulation of interleukin-12 production)|go:"GO:0032752"(positive regulation of interleukin-3 production)|go:"GO:0032753"(positive regulation of interleukin-4 production)|go:"GO:0032755"(positive regulation of interleukin-6 production)|go:"GO:0032757"(positive regulation of interleukin-8 production)|go:"GO:0032760"(positive regulation of tumor necrosis factor production)|go:"GO:0032928"(regulation of superoxide anion generation)|go:"GO:0010803"(regulation of tumor necrosis factor-mediated signaling pathway)|go:"GO:0016170"(interleukin-15 receptor binding)|go:"GO:0018105"(peptidyl-serine phosphorylation)|go:"GO:0032930"(positive regulation of superoxide anion generation)|go:"GO:0018108"(peptidyl-tyrosine phosphorylation)|go:"GO:0019370"(leukotriene biosynthetic process)|go:"GO:0032991"(protein-containing complex)|go:"GO:0033630"(positive regulation of cell adhesion mediated by integrin)|go:"GO:0043313"(regulation of neutrophil degranulation)|go:"GO:0043366"(beta selection)|go:"GO:0043410"(positive regulation of MAPK cascade)|go:"GO:0035325"(Toll-like receptor binding)|go:"GO:0035556"(intracellular signal transduction)|go:"GO:0043507"(positive regulation of JUN kinase activity)|go:"GO:0045087"(innate immune response)|go:"GO:0045579"(positive regulation of B cell differentiation)|go:"GO:0045588"(positive regulation of gamma-delta T cell differentiation)|go:"GO:0038063"(collagen-activated tyrosine kinase receptor signaling pathway)|go:"GO:0045780"(positive regulation of bone resorption)|go:"GO:0046638"(positive regulation of alpha-beta T cell differentiation)|go:"GO:0046641"(positive regulation of alpha-beta T cell proliferation)|go:"GO:0046777"(protein autophosphorylation)|go:"GO:0048514"(blood vessel morphogenesis)|go:"GO:0050731"(positive regulation of peptidyl-tyrosine phosphorylation)|go:"GO:0050764"(regulation of phagocytosis)|go:"GO:0050776"(regulation of immune response)|go:"GO:0050850"(positive regulation of calcium-mediated signaling)|go:"GO:0050853"(B cell receptor signaling pathway)|go:"GO:0051090"(regulation of DNA-binding transcription factor activity)|go:"GO:0038156"(interleukin-3-mediated signaling pathway)|go:"GO:0051712"(positive regulation of killing of cells of another organism)|go:"GO:0070372"(regulation of ERK1 and ERK2 cascade)|go:"GO:0042101"(T cell receptor complex)|go:"GO:0071226"(cellular response to molecule of fungal origin)|go:"GO:0042169"(SH2 domain binding)|go:"GO:0042742"(defense response to bacterium)|go:"GO:0071396"(cellular response to lipid)|go:"GO:0071404"(cellular response to low-density lipoprotein particle stimulus)|go:"GO:0043274"(phospholipase binding)|go:"GO:0043280"(positive regulation of cysteine-type endopeptidase activity involved in apoptotic process)|go:"GO:0043306"(positive regulation of mast cell degranulation)|go:"GO:0090237"(regulation of arachidonic acid secretion)|go:"GO:0090330"(regulation of platelet aggregation)|go:"GO:0097110"(scaffold protein binding)|go:"GO:0120162"(positive regulation of cold-induced thermogenesis)|go:"GO:1990858"(cellular response to lectin)|interpro:IPR000719(Protein kinase, core)|interpro:IPR000980(SH2 motif)|interpro:IPR001245(Tyrosine protein kinase)|interpro:IPR008266(Tyrosine protein kinase, active site)|interpro:IPR011009(Protein kinase-like)|interpro:IPR012234(Tyrosine protein kinase, SYK/ZAP-70)|interpro:IPR017441(Protein kinase ATP binding, conserved site)|interpro:IPR020635|interpro:IPR023420|interpro:IPR035838|interpro:IPR036860|mint:P48025|rcsb pdb:2LCT|rcsb pdb:2MC1|reactome:R-MMU-114604|reactome:R-MMU-2029481|reactome:R-MMU-2029482|reactome:R-MMU-2029485|reactome:R-MMU-2424491|reactome:R-MMU-2454202|reactome:R-MMU-2730905|reactome:R-MMU-2871796|reactome:R-MMU-2871809|reactome:R-MMU-354192|reactome:R-MMU-5621480|reactome:R-MMU-9020558|reactome:R-MMU-912631|reactome:R-MMU-9674555|reactome:R-MMU-9705462|reactome:R-MMU-9706374|reactome:R-MMU-983695|go:"GO:0071639"(positive regulation of monocyte chemotactic protein-1 production)|refseq:NP_001185906.1|refseq:NP_035648.2|refseq:XP_006516958.1|dip:DIP-32621N refseq:NP_031681.2|ensembl:ENSMUSG00000003379(gene)|ensembl:ENSMUST00000003469(transcript)|go:"GO:0002250"(adaptive immune response)|go:"GO:0004888"(transmembrane signaling receptor activity)|go:"GO:0005771"(multivesicular body)|go:"GO:0005886"(plasma membrane)|go:"GO:0009897"(external side of plasma membrane)|go:"GO:0016021"(integral component of membrane)|go:"GO:0019815"(B cell receptor complex)|go:"GO:0030183"(B cell differentiation)|go:"GO:0042100"(B cell proliferation)|go:"GO:0042113"(B cell activation)|go:"GO:0042802"(identical protein binding)|go:"GO:0045121"(membrane raft)|go:"GO:0050853"(B cell receptor signaling pathway)|interpro:IPR003110(Phosphorylated immunoreceptor signaling ITAM)|interpro:IPR003599(Immunoglobulin subtype)|interpro:IPR007110(Immunoglobulin-like)|interpro:IPR013151(Immunoglobulin)|interpro:IPR013783(Immunoglobulin-like fold)|interpro:IPR036179|interpro:IPR039695|reactome:R-MMU-5690714|reactome:R-MMU-983695|dip:DIP-61170N - - - figure legend:5D|antagonist:"pervanadate (Perv) - activator of the BCR."|comment:When permeabilised cells were pretreated with R406 - a Syk inhibitor, prior to LatA treatment, the interaction is inhibited. Pre-treatment with Src-family kinase inhibitor PP2 only delays a positive response.|agonist:Lat-A - activator of the BCR.|dataset:Affinomics - Interactions curated for the Affinomics consortium.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:10090(mouse-spleen)|taxid:10090(Mus musculus spleen) - 2015/02/13 2015/03/05 rogid:ojAGyI+gLZF5ThLd+bsHfMdjaM010090 rogid:VBxrmN49336aEFxa3XdHmbb6IjQ10090 intact-crc:F1B33ACC5A666F2A|rigid:FGfvfpUw+SZY9rlcKFP2YVk7y8Y false - - - - psi-mi:"MI:0421"(identification by antibody) psi-mi:"MI:0421"(identification by antibody) uniprotkb:P01882 uniprotkb:P01882 intact:EBI-10683684 intact:EBI-10683684 psi-mi:ighdm_mouse(display_long) psi-mi:ighdm_mouse(display_long) psi-mi:"MI:0813"(proximity ligation assay) Kläsener et al. (2014) imex:IM-23964|pubmed:24963139 taxid:10090(mouse)|taxid:10090(Mus musculus) taxid:10090(mouse)|taxid:10090(Mus musculus) psi-mi:"MI:2364"(proximity) psi-mi:"MI:0469"(IntAct) intact:EBI-10683700|imex:IM-23964-49 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) interpro:IPR007110(Immunoglobulin-like)|interpro:IPR013783(Immunoglobulin-like fold)|interpro:IPR003006(Immunoglobulin/major histocompatibility complex, conserved site)|interpro:IPR003597(Immunoglobulin C1-set)|go:"GO:0005886"(plasma membrane)|go:"GO:0016021"(integral component of membrane)|interpro:IPR036179 interpro:IPR007110(Immunoglobulin-like)|interpro:IPR013783(Immunoglobulin-like fold)|interpro:IPR003006(Immunoglobulin/major histocompatibility complex, conserved site)|interpro:IPR003597(Immunoglobulin C1-set)|go:"GO:0005886"(plasma membrane)|go:"GO:0016021"(integral component of membrane)|interpro:IPR036179 - - - figure legend:4A/C|comment:The Fab-PLA signal is lost upon exposure of B cells to antigen, or in presence of BCR-activating drugs.|antagonist:"specific antigen-NIP, pervanadate (Perv) and Lat-A - activators of the BCR."|dataset:Affinomics - Interactions curated for the Affinomics consortium.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:10090(mouse-spleen)|taxid:10090(Mus musculus spleen) - 2015/02/13 2015/02/24 rogid:WPb0XrDhxuYDQ0jFtnq2QDd6u1c10090 rogid:WPb0XrDhxuYDQ0jFtnq2QDd6u1c10090 intact-crc:D2FA6CEBA71EDDD4|rigid:Kbyvm7r3c+tkujEdaJCVrV1B61A false - - - - psi-mi:"MI:0421"(identification by antibody) psi-mi:"MI:0421"(identification by antibody) uniprotkb:P01872 uniprotkb:P25918 intact:EBI-645833|uniprotkb:P01873 intact:EBI-644463|uniprotkb:Q542B2|ensembl:ENSMUSP00000145803 psi-mi:ighm_mouse(display_long)|uniprotkb:Ighm(gene name)|psi-mi:Ighm(display_short)|uniprotkb:Igh-6(gene name synonym) psi-mi:cd19_mouse(display_long)|uniprotkb:Differentiation antigen CD19(gene name synonym)|uniprotkb:Cd19(gene name)|psi-mi:Cd19(display_short) psi-mi:"MI:0813"(proximity ligation assay) Kläsener et al. (2014) imex:IM-23964|pubmed:24963139 taxid:10090(mouse)|taxid:10090(Mus musculus) taxid:10090(mouse)|taxid:10090(Mus musculus) psi-mi:"MI:2364"(proximity) psi-mi:"MI:0469"(IntAct) intact:EBI-10683938|imex:IM-23964-37 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) interpro:IPR007110(Immunoglobulin-like)|interpro:IPR013783(Immunoglobulin-like fold)|interpro:IPR003006(Immunoglobulin/major histocompatibility complex, conserved site)|interpro:IPR003597(Immunoglobulin C1-set)|go:"GO:0002344"(B cell affinity maturation)|go:"GO:0002455"(humoral immune response mediated by circulating immunoglobulin)|go:"GO:0002637"(regulation of immunoglobulin production)|go:"GO:0003823"(antigen binding)|go:"GO:0004888"(transmembrane signaling receptor activity)|go:"GO:0005615"(extracellular space)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0006910"(phagocytosis, recognition)|go:"GO:0006911"(phagocytosis, engulfment)|go:"GO:0006958"(complement activation, classical pathway)|go:"GO:0009897"(external side of plasma membrane)|go:"GO:0009986"(cell surface)|go:"GO:0016020"(membrane)|go:"GO:0016021"(integral component of membrane)|go:"GO:0016064"(immunoglobulin mediated immune response)|go:"GO:0019815"(B cell receptor complex)|go:"GO:0019882"(antigen processing and presentation)|go:"GO:0022604"(regulation of cell morphogenesis)|go:"GO:0030890"(positive regulation of B cell proliferation)|go:"GO:0034987"(immunoglobulin receptor binding)|go:"GO:0042571"(immunoglobulin complex, circulating)|go:"GO:0042742"(defense response to bacterium)|go:"GO:0042802"(identical protein binding)|go:"GO:0043410"(positive regulation of MAPK cascade)|go:"GO:0045022"(early endosome to late endosome transport)|go:"GO:0045087"(innate immune response)|go:"GO:0045807"(positive regulation of endocytosis)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0050731"(positive regulation of peptidyl-tyrosine phosphorylation)|go:"GO:0050778"(positive regulation of immune response)|go:"GO:0050829"(defense response to Gram-negative bacterium)|go:"GO:0050853"(B cell receptor signaling pathway)|go:"GO:0050871"(positive regulation of B cell activation)|rcsb pdb:4BA8|rcsb pdb:4JVU|rcsb pdb:4JVW|reactome:R-MMU-202733|reactome:R-MMU-5690714|reactome:R-MMU-983695|interpro:IPR036179|mint:P01872 refseq:NP_033974.2|ensembl:ENSMUSG00000030724(gene)|ensembl:ENSMUST00000206325(transcript)|go:"GO:0001923"(B-1 B cell differentiation)|go:"GO:0002322"(B cell proliferation involved in immune response)|go:"GO:0005886"(plasma membrane)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0009897"(external side of plasma membrane)|go:"GO:0016064"(immunoglobulin mediated immune response)|go:"GO:0019724"(B cell mediated immunity)|go:"GO:0032991"(protein-containing complex)|go:"GO:0043552"(positive regulation of phosphatidylinositol 3-kinase activity)|go:"GO:0045121"(membrane raft)|go:"GO:0050851"(antigen receptor-mediated signaling pathway)|go:"GO:0050853"(B cell receptor signaling pathway)|go:"GO:0050855"(regulation of B cell receptor signaling pathway)|go:"GO:0050864"(regulation of B cell activation)|go:"GO:0051281"(positive regulation of release of sequestered calcium ion into cytosol)|interpro:IPR003599(Immunoglobulin subtype)|interpro:IPR007110(Immunoglobulin-like)|interpro:IPR013783(Immunoglobulin-like fold)|interpro:IPR036179|interpro:IPR042341|reactome:R-MMU-1257604|reactome:R-MMU-198933|reactome:R-MMU-6811558|reactome:R-MMU-977606|reactome:R-MMU-983695|refseq:XP_006507346.1 - - - figure legend:Figure 7B Supplementary 1|comment:IgM-BCR only gains contact to the CD19/CD20 module upon B cell activation.|agonist:Specific antigen- NIP, pervanadate and Lat-A- - activators of the BCR|dataset:Affinomics - Interactions curated for the Affinomics consortium.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:10090(mouse-tko-pre-b-cells)|taxid:10090("Mouse Triple_ Knock_out pre-B cells:SLP-65,Rag2, CD179b deficient") - 2015/02/13 2015/02/24 rogid:4+PnUlhyUfPZS43gGZMtyePhlCw10090 rogid:YWZCIrT3MOdFhZd5+gTZhMkF8yc10090 intact-crc:1A95FF1BAE15E84D|rigid:A/Bc70lm3EejkcIeAaEQImf+Ntg false - - - - psi-mi:"MI:0421"(identification by antibody) psi-mi:"MI:0421"(identification by antibody) uniprotkb:P19437 uniprotkb:P01872 intact:EBI-10683749|ensembl:ENSMUSP00000126422 intact:EBI-645833|uniprotkb:P01873 psi-mi:cd20_mouse(display_long)|uniprotkb:Ms4a1(gene name)|psi-mi:Ms4a1(display_short)|uniprotkb:Cd20(gene name synonym)|uniprotkb:Ly-44(gene name synonym)|uniprotkb:Ms4a2(gene name synonym)|uniprotkb:B-cell differentiation antigen Ly-44(gene name synonym)|uniprotkb:Lymphocyte antigen 44(gene name synonym)|uniprotkb:Membrane-spanning 4-domains subfamily A member 1(gene name synonym) psi-mi:ighm_mouse(display_long)|uniprotkb:Ighm(gene name)|psi-mi:Ighm(display_short)|uniprotkb:Igh-6(gene name synonym) psi-mi:"MI:0813"(proximity ligation assay) Kläsener et al. (2014) imex:IM-23964|pubmed:24963139 taxid:10090(mouse)|taxid:10090(Mus musculus) taxid:10090(mouse)|taxid:10090(Mus musculus) psi-mi:"MI:2364"(proximity) psi-mi:"MI:0469"(IntAct) intact:EBI-10683945|imex:IM-23964-39 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) refseq:NP_031667.1|ensembl:ENSMUSG00000024673(gene)|ensembl:ENSMUST00000169159(transcript)|go:"GO:0002115"(store-operated calcium entry)|go:"GO:0005154"(epidermal growth factor receptor binding)|go:"GO:0005654"(nucleoplasm)|go:"GO:0005886"(plasma membrane)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0009617"(response to bacterium)|go:"GO:0009897"(external side of plasma membrane)|go:"GO:0009986"(cell surface)|go:"GO:0019865"(immunoglobulin binding)|go:"GO:0030183"(B cell differentiation)|go:"GO:0042113"(B cell activation)|go:"GO:0042802"(identical protein binding)|go:"GO:0044853"(plasma membrane raft)|go:"GO:0050853"(B cell receptor signaling pathway)|go:"GO:0051262"(protein tetramerization)|go:"GO:1902656"(calcium ion import into cytosol)|interpro:IPR007237(CD20/IgE Fc receptor beta subunit)|interpro:IPR030417|interpro:IPR030418 interpro:IPR007110(Immunoglobulin-like)|interpro:IPR013783(Immunoglobulin-like fold)|interpro:IPR003006(Immunoglobulin/major histocompatibility complex, conserved site)|interpro:IPR003597(Immunoglobulin C1-set)|go:"GO:0002344"(B cell affinity maturation)|go:"GO:0002455"(humoral immune response mediated by circulating immunoglobulin)|go:"GO:0002637"(regulation of immunoglobulin production)|go:"GO:0003823"(antigen binding)|go:"GO:0004888"(transmembrane signaling receptor activity)|go:"GO:0005615"(extracellular space)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0006910"(phagocytosis, recognition)|go:"GO:0006911"(phagocytosis, engulfment)|go:"GO:0006958"(complement activation, classical pathway)|go:"GO:0009897"(external side of plasma membrane)|go:"GO:0009986"(cell surface)|go:"GO:0016020"(membrane)|go:"GO:0016021"(integral component of membrane)|go:"GO:0016064"(immunoglobulin mediated immune response)|go:"GO:0019815"(B cell receptor complex)|go:"GO:0019882"(antigen processing and presentation)|go:"GO:0022604"(regulation of cell morphogenesis)|go:"GO:0030890"(positive regulation of B cell proliferation)|go:"GO:0034987"(immunoglobulin receptor binding)|go:"GO:0042571"(immunoglobulin complex, circulating)|go:"GO:0042742"(defense response to bacterium)|go:"GO:0042802"(identical protein binding)|go:"GO:0043410"(positive regulation of MAPK cascade)|go:"GO:0045022"(early endosome to late endosome transport)|go:"GO:0045087"(innate immune response)|go:"GO:0045807"(positive regulation of endocytosis)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0050731"(positive regulation of peptidyl-tyrosine phosphorylation)|go:"GO:0050778"(positive regulation of immune response)|go:"GO:0050829"(defense response to Gram-negative bacterium)|go:"GO:0050853"(B cell receptor signaling pathway)|go:"GO:0050871"(positive regulation of B cell activation)|rcsb pdb:4BA8|rcsb pdb:4JVU|rcsb pdb:4JVW|reactome:R-MMU-202733|reactome:R-MMU-5690714|reactome:R-MMU-983695|interpro:IPR036179|mint:P01872 - - - figure legend:Figure 7C Supplementary 1|comment:IgM-BCR only gains contact to the CD19/CD20 module upon B cell activation.|agonist:Specific antigen- NIP, pervanadate and Lat-A- - activators of the BCR|dataset:Affinomics - Interactions curated for the Affinomics consortium.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:10090(mouse-tko-pre-b-cells)|taxid:10090("Mouse Triple_ Knock_out pre-B cells:SLP-65,Rag2, CD179b deficient") - 2015/02/13 2015/02/24 rogid:GPiXJuTEbnfqoUhQ0apzTyBcbzg10090 rogid:4+PnUlhyUfPZS43gGZMtyePhlCw10090 intact-crc:FA24FF1AAED333E6|rigid:eOiDaeqdonr2Q7LU76dmHoQK5I0 false - - - - psi-mi:"MI:0421"(identification by antibody) psi-mi:"MI:0421"(identification by antibody) uniprotkb:P01872 uniprotkb:P01872 intact:EBI-645833|uniprotkb:P01873 intact:EBI-645833|uniprotkb:P01873 psi-mi:ighm_mouse(display_long)|uniprotkb:Ighm(gene name)|psi-mi:Ighm(display_short)|uniprotkb:Igh-6(gene name synonym) psi-mi:ighm_mouse(display_long)|uniprotkb:Ighm(gene name)|psi-mi:Ighm(display_short)|uniprotkb:Igh-6(gene name synonym) psi-mi:"MI:0813"(proximity ligation assay) Kläsener et al. (2014) imex:IM-23964|pubmed:24963139 taxid:10090(mouse)|taxid:10090(Mus musculus) taxid:10090(mouse)|taxid:10090(Mus musculus) psi-mi:"MI:2364"(proximity) psi-mi:"MI:0469"(IntAct) intact:EBI-10683901|imex:IM-23964-67 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) interpro:IPR007110(Immunoglobulin-like)|interpro:IPR013783(Immunoglobulin-like fold)|interpro:IPR003006(Immunoglobulin/major histocompatibility complex, conserved site)|interpro:IPR003597(Immunoglobulin C1-set)|go:"GO:0002344"(B cell affinity maturation)|go:"GO:0002455"(humoral immune response mediated by circulating immunoglobulin)|go:"GO:0002637"(regulation of immunoglobulin production)|go:"GO:0003823"(antigen binding)|go:"GO:0004888"(transmembrane signaling receptor activity)|go:"GO:0005615"(extracellular space)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0006910"(phagocytosis, recognition)|go:"GO:0006911"(phagocytosis, engulfment)|go:"GO:0006958"(complement activation, classical pathway)|go:"GO:0009897"(external side of plasma membrane)|go:"GO:0009986"(cell surface)|go:"GO:0016020"(membrane)|go:"GO:0016021"(integral component of membrane)|go:"GO:0016064"(immunoglobulin mediated immune response)|go:"GO:0019815"(B cell receptor complex)|go:"GO:0019882"(antigen processing and presentation)|go:"GO:0022604"(regulation of cell morphogenesis)|go:"GO:0030890"(positive regulation of B cell proliferation)|go:"GO:0034987"(immunoglobulin receptor binding)|go:"GO:0042571"(immunoglobulin complex, circulating)|go:"GO:0042742"(defense response to bacterium)|go:"GO:0042802"(identical protein binding)|go:"GO:0043410"(positive regulation of MAPK cascade)|go:"GO:0045022"(early endosome to late endosome transport)|go:"GO:0045087"(innate immune response)|go:"GO:0045807"(positive regulation of endocytosis)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0050731"(positive regulation of peptidyl-tyrosine phosphorylation)|go:"GO:0050778"(positive regulation of immune response)|go:"GO:0050829"(defense response to Gram-negative bacterium)|go:"GO:0050853"(B cell receptor signaling pathway)|go:"GO:0050871"(positive regulation of B cell activation)|rcsb pdb:4BA8|rcsb pdb:4JVU|rcsb pdb:4JVW|reactome:R-MMU-202733|reactome:R-MMU-5690714|reactome:R-MMU-983695|interpro:IPR036179|mint:P01872 interpro:IPR007110(Immunoglobulin-like)|interpro:IPR013783(Immunoglobulin-like fold)|interpro:IPR003006(Immunoglobulin/major histocompatibility complex, conserved site)|interpro:IPR003597(Immunoglobulin C1-set)|go:"GO:0002344"(B cell affinity maturation)|go:"GO:0002455"(humoral immune response mediated by circulating immunoglobulin)|go:"GO:0002637"(regulation of immunoglobulin production)|go:"GO:0003823"(antigen binding)|go:"GO:0004888"(transmembrane signaling receptor activity)|go:"GO:0005615"(extracellular space)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0006910"(phagocytosis, recognition)|go:"GO:0006911"(phagocytosis, engulfment)|go:"GO:0006958"(complement activation, classical pathway)|go:"GO:0009897"(external side of plasma membrane)|go:"GO:0009986"(cell surface)|go:"GO:0016020"(membrane)|go:"GO:0016021"(integral component of membrane)|go:"GO:0016064"(immunoglobulin mediated immune response)|go:"GO:0019815"(B cell receptor complex)|go:"GO:0019882"(antigen processing and presentation)|go:"GO:0022604"(regulation of cell morphogenesis)|go:"GO:0030890"(positive regulation of B cell proliferation)|go:"GO:0034987"(immunoglobulin receptor binding)|go:"GO:0042571"(immunoglobulin complex, circulating)|go:"GO:0042742"(defense response to bacterium)|go:"GO:0042802"(identical protein binding)|go:"GO:0043410"(positive regulation of MAPK cascade)|go:"GO:0045022"(early endosome to late endosome transport)|go:"GO:0045087"(innate immune response)|go:"GO:0045807"(positive regulation of endocytosis)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0050731"(positive regulation of peptidyl-tyrosine phosphorylation)|go:"GO:0050778"(positive regulation of immune response)|go:"GO:0050829"(defense response to Gram-negative bacterium)|go:"GO:0050853"(B cell receptor signaling pathway)|go:"GO:0050871"(positive regulation of B cell activation)|rcsb pdb:4BA8|rcsb pdb:4JVU|rcsb pdb:4JVW|reactome:R-MMU-202733|reactome:R-MMU-5690714|reactome:R-MMU-983695|interpro:IPR036179|mint:P01872 - - - figure legend:5G, Figure 7A Supplementary 1|comment:"A clear difference was shown between the relative IgM:IgM conformation or distance between resting (positive result) and activated B cells (negative result). The Fab-PLA signal is lost upon exposure of B cells to antigen (NIP), or in presence of BCR-activating drugs."|antagonist:Specific antigen- NIP, pervanadate and Lat-A.|comment:More NIP is required to lose the positive PLA signal after Syk inhibitor R406 treatment.|dataset:Affinomics - Interactions curated for the Affinomics consortium.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:10090(mouse-tko-pre-b-cells)|taxid:10090("Mouse Triple_ Knock_out pre-B cells:SLP-65,Rag2, CD179b deficient") - 2015/02/13 2015/02/24 rogid:4+PnUlhyUfPZS43gGZMtyePhlCw10090 rogid:4+PnUlhyUfPZS43gGZMtyePhlCw10090 intact-crc:E8834F3F069D6A27|rigid:N/cK5ET0O9C0NgHLucUfovmg9nc false - - - - psi-mi:"MI:0421"(identification by antibody) psi-mi:"MI:0421"(identification by antibody) uniprotkb:Q64389 uniprotkb:P01872 intact:EBI-10683966|ensembl:ENSMUSP00000000696 intact:EBI-645833|uniprotkb:P01873 psi-mi:cd52_mouse(display_long)|uniprotkb:Cd52(gene name)|psi-mi:Cd52(display_short)|uniprotkb:Cdw52(gene name synonym)|uniprotkb:Mb7(gene name synonym)|uniprotkb:Lymphocyte differentiation antigen B7(gene name synonym) psi-mi:ighm_mouse(display_long)|uniprotkb:Ighm(gene name)|psi-mi:Ighm(display_short)|uniprotkb:Igh-6(gene name synonym) psi-mi:"MI:0813"(proximity ligation assay) Kläsener et al. (2014) imex:IM-23964|pubmed:24963139 taxid:10090(mouse)|taxid:10090(Mus musculus) taxid:10090(mouse)|taxid:10090(Mus musculus) psi-mi:"MI:2364"(proximity) psi-mi:"MI:0469"(IntAct) intact:EBI-10683960|imex:IM-23964-17 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) refseq:NP_038734.1|ensembl:ENSMUSG00000000682(gene)|ensembl:ENSMUST00000000696(transcript)|go:"GO:0005886"(plasma membrane)|go:"GO:0007204"(positive regulation of cytosolic calcium ion concentration)|go:"GO:0009617"(response to bacterium)|go:"GO:0031225"(anchored component of membrane)|go:"GO:0097225"(sperm midpiece)|interpro:IPR026643|reactome:R-MMU-163125 interpro:IPR007110(Immunoglobulin-like)|interpro:IPR013783(Immunoglobulin-like fold)|interpro:IPR003006(Immunoglobulin/major histocompatibility complex, conserved site)|interpro:IPR003597(Immunoglobulin C1-set)|go:"GO:0002344"(B cell affinity maturation)|go:"GO:0002455"(humoral immune response mediated by circulating immunoglobulin)|go:"GO:0002637"(regulation of immunoglobulin production)|go:"GO:0003823"(antigen binding)|go:"GO:0004888"(transmembrane signaling receptor activity)|go:"GO:0005615"(extracellular space)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0006910"(phagocytosis, recognition)|go:"GO:0006911"(phagocytosis, engulfment)|go:"GO:0006958"(complement activation, classical pathway)|go:"GO:0009897"(external side of plasma membrane)|go:"GO:0009986"(cell surface)|go:"GO:0016020"(membrane)|go:"GO:0016021"(integral component of membrane)|go:"GO:0016064"(immunoglobulin mediated immune response)|go:"GO:0019815"(B cell receptor complex)|go:"GO:0019882"(antigen processing and presentation)|go:"GO:0022604"(regulation of cell morphogenesis)|go:"GO:0030890"(positive regulation of B cell proliferation)|go:"GO:0034987"(immunoglobulin receptor binding)|go:"GO:0042571"(immunoglobulin complex, circulating)|go:"GO:0042742"(defense response to bacterium)|go:"GO:0042802"(identical protein binding)|go:"GO:0043410"(positive regulation of MAPK cascade)|go:"GO:0045022"(early endosome to late endosome transport)|go:"GO:0045087"(innate immune response)|go:"GO:0045807"(positive regulation of endocytosis)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0050731"(positive regulation of peptidyl-tyrosine phosphorylation)|go:"GO:0050778"(positive regulation of immune response)|go:"GO:0050829"(defense response to Gram-negative bacterium)|go:"GO:0050853"(B cell receptor signaling pathway)|go:"GO:0050871"(positive regulation of B cell activation)|rcsb pdb:4BA8|rcsb pdb:4JVU|rcsb pdb:4JVW|reactome:R-MMU-202733|reactome:R-MMU-5690714|reactome:R-MMU-983695|interpro:IPR036179|mint:P01872 - - - figure legend:Figure 7B Supplementary 2|agonist:Specific antigen- NIP.|dataset:Affinomics - Interactions curated for the Affinomics consortium.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:10090(mouse-tko-pre-b-cells)|taxid:10090("Mouse Triple_ Knock_out pre-B cells:SLP-65,Rag2, CD179b deficient") - 2015/02/13 2015/02/24 rogid:QKz1onjxDperltn/dTei86qYFGo10090 rogid:4+PnUlhyUfPZS43gGZMtyePhlCw10090 intact-crc:7BC8FF5E6EE7C8ED|rigid:IoEMy4opQ373zBtLVEJttDR50Og false - - - - psi-mi:"MI:0421"(identification by antibody) psi-mi:"MI:0421"(identification by antibody) uniprotkb:P01872 uniprotkb:P01872 intact:EBI-645833|uniprotkb:P01873 intact:EBI-645833|uniprotkb:P01873 psi-mi:ighm_mouse(display_long)|uniprotkb:Ighm(gene name)|psi-mi:Ighm(display_short)|uniprotkb:Igh-6(gene name synonym) psi-mi:ighm_mouse(display_long)|uniprotkb:Ighm(gene name)|psi-mi:Ighm(display_short)|uniprotkb:Igh-6(gene name synonym) psi-mi:"MI:0054"(fluorescence-activated cell sorting) Kläsener et al. (2014) imex:IM-23964|pubmed:24963139 taxid:10090(mouse)|taxid:10090(Mus musculus) taxid:10090(mouse)|taxid:10090(Mus musculus) psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-10683380|imex:IM-23964-1 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) interpro:IPR007110(Immunoglobulin-like)|interpro:IPR013783(Immunoglobulin-like fold)|interpro:IPR003006(Immunoglobulin/major histocompatibility complex, conserved site)|interpro:IPR003597(Immunoglobulin C1-set)|go:"GO:0002344"(B cell affinity maturation)|go:"GO:0002455"(humoral immune response mediated by circulating immunoglobulin)|go:"GO:0002637"(regulation of immunoglobulin production)|go:"GO:0003823"(antigen binding)|go:"GO:0004888"(transmembrane signaling receptor activity)|go:"GO:0005615"(extracellular space)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0006910"(phagocytosis, recognition)|go:"GO:0006911"(phagocytosis, engulfment)|go:"GO:0006958"(complement activation, classical pathway)|go:"GO:0009897"(external side of plasma membrane)|go:"GO:0009986"(cell surface)|go:"GO:0016020"(membrane)|go:"GO:0016021"(integral component of membrane)|go:"GO:0016064"(immunoglobulin mediated immune response)|go:"GO:0019815"(B cell receptor complex)|go:"GO:0019882"(antigen processing and presentation)|go:"GO:0022604"(regulation of cell morphogenesis)|go:"GO:0030890"(positive regulation of B cell proliferation)|go:"GO:0034987"(immunoglobulin receptor binding)|go:"GO:0042571"(immunoglobulin complex, circulating)|go:"GO:0042742"(defense response to bacterium)|go:"GO:0042802"(identical protein binding)|go:"GO:0043410"(positive regulation of MAPK cascade)|go:"GO:0045022"(early endosome to late endosome transport)|go:"GO:0045087"(innate immune response)|go:"GO:0045807"(positive regulation of endocytosis)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0050731"(positive regulation of peptidyl-tyrosine phosphorylation)|go:"GO:0050778"(positive regulation of immune response)|go:"GO:0050829"(defense response to Gram-negative bacterium)|go:"GO:0050853"(B cell receptor signaling pathway)|go:"GO:0050871"(positive regulation of B cell activation)|rcsb pdb:4BA8|rcsb pdb:4JVU|rcsb pdb:4JVW|reactome:R-MMU-202733|reactome:R-MMU-5690714|reactome:R-MMU-983695|interpro:IPR036179|mint:P01872 interpro:IPR007110(Immunoglobulin-like)|interpro:IPR013783(Immunoglobulin-like fold)|interpro:IPR003006(Immunoglobulin/major histocompatibility complex, conserved site)|interpro:IPR003597(Immunoglobulin C1-set)|go:"GO:0002344"(B cell affinity maturation)|go:"GO:0002455"(humoral immune response mediated by circulating immunoglobulin)|go:"GO:0002637"(regulation of immunoglobulin production)|go:"GO:0003823"(antigen binding)|go:"GO:0004888"(transmembrane signaling receptor activity)|go:"GO:0005615"(extracellular space)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0006910"(phagocytosis, recognition)|go:"GO:0006911"(phagocytosis, engulfment)|go:"GO:0006958"(complement activation, classical pathway)|go:"GO:0009897"(external side of plasma membrane)|go:"GO:0009986"(cell surface)|go:"GO:0016020"(membrane)|go:"GO:0016021"(integral component of membrane)|go:"GO:0016064"(immunoglobulin mediated immune response)|go:"GO:0019815"(B cell receptor complex)|go:"GO:0019882"(antigen processing and presentation)|go:"GO:0022604"(regulation of cell morphogenesis)|go:"GO:0030890"(positive regulation of B cell proliferation)|go:"GO:0034987"(immunoglobulin receptor binding)|go:"GO:0042571"(immunoglobulin complex, circulating)|go:"GO:0042742"(defense response to bacterium)|go:"GO:0042802"(identical protein binding)|go:"GO:0043410"(positive regulation of MAPK cascade)|go:"GO:0045022"(early endosome to late endosome transport)|go:"GO:0045087"(innate immune response)|go:"GO:0045807"(positive regulation of endocytosis)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0050731"(positive regulation of peptidyl-tyrosine phosphorylation)|go:"GO:0050778"(positive regulation of immune response)|go:"GO:0050829"(defense response to Gram-negative bacterium)|go:"GO:0050853"(B cell receptor signaling pathway)|go:"GO:0050871"(positive regulation of B cell activation)|rcsb pdb:4BA8|rcsb pdb:4JVU|rcsb pdb:4JVW|reactome:R-MMU-202733|reactome:R-MMU-5690714|reactome:R-MMU-983695|interpro:IPR036179|mint:P01872 - - - figure legend:2C-2|comment:Fab-PLA detects the pentameric IgM but not the loosely spaced monomeric IgM. Fab-PLA specifically detects oligomeric forms of IgM be it the soluble IgM pentamer or the oligomeric IgM-BCR on the B cell surface.|dataset:Affinomics - Interactions curated for the Affinomics consortium.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/02/13 2015/02/24 rogid:4+PnUlhyUfPZS43gGZMtyePhlCw10090 rogid:4+PnUlhyUfPZS43gGZMtyePhlCw10090 intact-crc:F0BBD93695973533|rigid:Ds7woaMkohaLVQdhA+Oztdzk4Vk false - - 5 0 psi-mi:"MI:0396"(predetermined participant) psi-mi:"MI:0396"(predetermined participant) uniprotkb:P01872 uniprotkb:P01872 intact:EBI-645833|uniprotkb:P01873 intact:EBI-645833|uniprotkb:P01873 psi-mi:ighm_mouse(display_long)|uniprotkb:Ighm(gene name)|psi-mi:Ighm(display_short)|uniprotkb:Igh-6(gene name synonym) psi-mi:ighm_mouse(display_long)|uniprotkb:Ighm(gene name)|psi-mi:Ighm(display_short)|uniprotkb:Igh-6(gene name synonym) psi-mi:"MI:0813"(proximity ligation assay) Kläsener et al. (2014) imex:IM-23964|pubmed:24963139 taxid:10090(mouse)|taxid:10090(Mus musculus) taxid:10090(mouse)|taxid:10090(Mus musculus) psi-mi:"MI:2364"(proximity) psi-mi:"MI:0469"(IntAct) intact:EBI-10683283|imex:IM-23964-21 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) interpro:IPR007110(Immunoglobulin-like)|interpro:IPR013783(Immunoglobulin-like fold)|interpro:IPR003006(Immunoglobulin/major histocompatibility complex, conserved site)|interpro:IPR003597(Immunoglobulin C1-set)|go:"GO:0002344"(B cell affinity maturation)|go:"GO:0002455"(humoral immune response mediated by circulating immunoglobulin)|go:"GO:0002637"(regulation of immunoglobulin production)|go:"GO:0003823"(antigen binding)|go:"GO:0004888"(transmembrane signaling receptor activity)|go:"GO:0005615"(extracellular space)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0006910"(phagocytosis, recognition)|go:"GO:0006911"(phagocytosis, engulfment)|go:"GO:0006958"(complement activation, classical pathway)|go:"GO:0009897"(external side of plasma membrane)|go:"GO:0009986"(cell surface)|go:"GO:0016020"(membrane)|go:"GO:0016021"(integral component of membrane)|go:"GO:0016064"(immunoglobulin mediated immune response)|go:"GO:0019815"(B cell receptor complex)|go:"GO:0019882"(antigen processing and presentation)|go:"GO:0022604"(regulation of cell morphogenesis)|go:"GO:0030890"(positive regulation of B cell proliferation)|go:"GO:0034987"(immunoglobulin receptor binding)|go:"GO:0042571"(immunoglobulin complex, circulating)|go:"GO:0042742"(defense response to bacterium)|go:"GO:0042802"(identical protein binding)|go:"GO:0043410"(positive regulation of MAPK cascade)|go:"GO:0045022"(early endosome to late endosome transport)|go:"GO:0045087"(innate immune response)|go:"GO:0045807"(positive regulation of endocytosis)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0050731"(positive regulation of peptidyl-tyrosine phosphorylation)|go:"GO:0050778"(positive regulation of immune response)|go:"GO:0050829"(defense response to Gram-negative bacterium)|go:"GO:0050853"(B cell receptor signaling pathway)|go:"GO:0050871"(positive regulation of B cell activation)|rcsb pdb:4BA8|rcsb pdb:4JVU|rcsb pdb:4JVW|reactome:R-MMU-202733|reactome:R-MMU-5690714|reactome:R-MMU-983695|interpro:IPR036179|mint:P01872 interpro:IPR007110(Immunoglobulin-like)|interpro:IPR013783(Immunoglobulin-like fold)|interpro:IPR003006(Immunoglobulin/major histocompatibility complex, conserved site)|interpro:IPR003597(Immunoglobulin C1-set)|go:"GO:0002344"(B cell affinity maturation)|go:"GO:0002455"(humoral immune response mediated by circulating immunoglobulin)|go:"GO:0002637"(regulation of immunoglobulin production)|go:"GO:0003823"(antigen binding)|go:"GO:0004888"(transmembrane signaling receptor activity)|go:"GO:0005615"(extracellular space)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0006910"(phagocytosis, recognition)|go:"GO:0006911"(phagocytosis, engulfment)|go:"GO:0006958"(complement activation, classical pathway)|go:"GO:0009897"(external side of plasma membrane)|go:"GO:0009986"(cell surface)|go:"GO:0016020"(membrane)|go:"GO:0016021"(integral component of membrane)|go:"GO:0016064"(immunoglobulin mediated immune response)|go:"GO:0019815"(B cell receptor complex)|go:"GO:0019882"(antigen processing and presentation)|go:"GO:0022604"(regulation of cell morphogenesis)|go:"GO:0030890"(positive regulation of B cell proliferation)|go:"GO:0034987"(immunoglobulin receptor binding)|go:"GO:0042571"(immunoglobulin complex, circulating)|go:"GO:0042742"(defense response to bacterium)|go:"GO:0042802"(identical protein binding)|go:"GO:0043410"(positive regulation of MAPK cascade)|go:"GO:0045022"(early endosome to late endosome transport)|go:"GO:0045087"(innate immune response)|go:"GO:0045807"(positive regulation of endocytosis)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0050731"(positive regulation of peptidyl-tyrosine phosphorylation)|go:"GO:0050778"(positive regulation of immune response)|go:"GO:0050829"(defense response to Gram-negative bacterium)|go:"GO:0050853"(B cell receptor signaling pathway)|go:"GO:0050871"(positive regulation of B cell activation)|rcsb pdb:4BA8|rcsb pdb:4JVU|rcsb pdb:4JVW|reactome:R-MMU-202733|reactome:R-MMU-5690714|reactome:R-MMU-983695|interpro:IPR036179|mint:P01872 - - - figure legend:1E|comment:"Results did not distinguish between resting and antigen (NIP) stimulated cells."|dataset:Affinomics - Interactions curated for the Affinomics consortium.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:10090(mouse-tko-md-pre-b cells)|taxid:10090("Mouse TKO_MD Pre B Cells:KO for SLP-65, RAG2, CD179b, transfected with NOP specific IgM/IgD BCR") - 2015/02/13 2015/02/24 rogid:4+PnUlhyUfPZS43gGZMtyePhlCw10090 rogid:4+PnUlhyUfPZS43gGZMtyePhlCw10090 intact-crc:DCD7CB61EB9C3277|rigid:N/cK5ET0O9C0NgHLucUfovmg9nc false - - - - psi-mi:"MI:0421"(identification by antibody) psi-mi:"MI:0421"(identification by antibody) uniprotkb:P01882 uniprotkb:P19437 intact:EBI-10683684 intact:EBI-10683749|ensembl:ENSMUSP00000126422 psi-mi:ighdm_mouse(display_long) psi-mi:cd20_mouse(display_long)|uniprotkb:Ms4a1(gene name)|psi-mi:Ms4a1(display_short)|uniprotkb:Cd20(gene name synonym)|uniprotkb:Ly-44(gene name synonym)|uniprotkb:Ms4a2(gene name synonym)|uniprotkb:B-cell differentiation antigen Ly-44(gene name synonym)|uniprotkb:Lymphocyte antigen 44(gene name synonym)|uniprotkb:Membrane-spanning 4-domains subfamily A member 1(gene name synonym) psi-mi:"MI:0813"(proximity ligation assay) Kläsener et al. (2014) imex:IM-23964|pubmed:24963139 taxid:10090(mouse)|taxid:10090(Mus musculus) taxid:10090(mouse)|taxid:10090(Mus musculus) psi-mi:"MI:2364"(proximity) psi-mi:"MI:0469"(IntAct) intact:EBI-10683932|imex:IM-23964-69 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) interpro:IPR007110(Immunoglobulin-like)|interpro:IPR013783(Immunoglobulin-like fold)|interpro:IPR003006(Immunoglobulin/major histocompatibility complex, conserved site)|interpro:IPR003597(Immunoglobulin C1-set)|go:"GO:0005886"(plasma membrane)|go:"GO:0016021"(integral component of membrane)|interpro:IPR036179 refseq:NP_031667.1|ensembl:ENSMUSG00000024673(gene)|ensembl:ENSMUST00000169159(transcript)|go:"GO:0002115"(store-operated calcium entry)|go:"GO:0005154"(epidermal growth factor receptor binding)|go:"GO:0005654"(nucleoplasm)|go:"GO:0005886"(plasma membrane)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0009617"(response to bacterium)|go:"GO:0009897"(external side of plasma membrane)|go:"GO:0009986"(cell surface)|go:"GO:0019865"(immunoglobulin binding)|go:"GO:0030183"(B cell differentiation)|go:"GO:0042113"(B cell activation)|go:"GO:0042802"(identical protein binding)|go:"GO:0044853"(plasma membrane raft)|go:"GO:0050853"(B cell receptor signaling pathway)|go:"GO:0051262"(protein tetramerization)|go:"GO:1902656"(calcium ion import into cytosol)|interpro:IPR007237(CD20/IgE Fc receptor beta subunit)|interpro:IPR030417|interpro:IPR030418 - - - figure legend:Figure 7F Supplementary 1|comment:"Resting TKO-D cells display a close proximity between the IgD-BCR and the CD19/CD20 module but lose this interaction upon BCR stimulation The Fab-PLA signal is lost upon exposure of B cells to antigen (NIP), or in presence of BCR-activating drugs."|antagonist:Specific antigen- NIP, pervanadate and Lat-A - activators of the BCR|dataset:Affinomics - Interactions curated for the Affinomics consortium.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:10090(mouse-tko-pre-b-cells)|taxid:10090("Mouse Triple_ Knock_out pre-B cells:SLP-65,Rag2, CD179b deficient") - 2015/02/13 2015/02/24 rogid:WPb0XrDhxuYDQ0jFtnq2QDd6u1c10090 rogid:GPiXJuTEbnfqoUhQ0apzTyBcbzg10090 intact-crc:145C5612368DA8C4|rigid:+ZdVbKS/sTYEGBe3RKOlZyer88E false - - - - psi-mi:"MI:0833"(autoradiography) psi-mi:"MI:0833"(autoradiography) uniprotkb:P01882 uniprotkb:P01882 intact:EBI-10683684 intact:EBI-10683684 psi-mi:ighdm_mouse(display_long) psi-mi:ighdm_mouse(display_long) psi-mi:"MI:0813"(proximity ligation assay) Kläsener et al. (2014) imex:IM-23964|pubmed:24963139 taxid:10090(mouse)|taxid:10090(Mus musculus) taxid:10090(mouse)|taxid:10090(Mus musculus) psi-mi:"MI:2364"(proximity) psi-mi:"MI:0469"(IntAct) intact:EBI-10683919|imex:IM-23964-15 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) interpro:IPR007110(Immunoglobulin-like)|interpro:IPR013783(Immunoglobulin-like fold)|interpro:IPR003006(Immunoglobulin/major histocompatibility complex, conserved site)|interpro:IPR003597(Immunoglobulin C1-set)|go:"GO:0005886"(plasma membrane)|go:"GO:0016021"(integral component of membrane)|interpro:IPR036179 interpro:IPR007110(Immunoglobulin-like)|interpro:IPR013783(Immunoglobulin-like fold)|interpro:IPR003006(Immunoglobulin/major histocompatibility complex, conserved site)|interpro:IPR003597(Immunoglobulin C1-set)|go:"GO:0005886"(plasma membrane)|go:"GO:0016021"(integral component of membrane)|interpro:IPR036179 - - - figure legend:Figure 7D Supplementary 1|comment:"The Fab-PLA signal is lost upon exposure of B cells to antigen (NIP), or in presence of BCR-activating drugs."|antagonist:Specific antigen- NIP, pervanadate and Lat-A - activators of the BCR|dataset:Affinomics - Interactions curated for the Affinomics consortium.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:10090(mouse-tko-pre-b-cells)|taxid:10090("Mouse Triple_ Knock_out pre-B cells:SLP-65,Rag2, CD179b deficient") - 2015/02/13 2015/02/24 rogid:WPb0XrDhxuYDQ0jFtnq2QDd6u1c10090 rogid:WPb0XrDhxuYDQ0jFtnq2QDd6u1c10090 intact-crc:0ED02C69FF4DCF10|rigid:Kbyvm7r3c+tkujEdaJCVrV1B61A false - - - - psi-mi:"MI:0833"(autoradiography) psi-mi:"MI:0833"(autoradiography) uniprotkb:P01882 uniprotkb:Q64389 intact:EBI-10683684 intact:EBI-10683966|ensembl:ENSMUSP00000000696 psi-mi:ighdm_mouse(display_long) psi-mi:cd52_mouse(display_long)|uniprotkb:Cd52(gene name)|psi-mi:Cd52(display_short)|uniprotkb:Cdw52(gene name synonym)|uniprotkb:Mb7(gene name synonym)|uniprotkb:Lymphocyte differentiation antigen B7(gene name synonym) psi-mi:"MI:0813"(proximity ligation assay) Kläsener et al. (2014) imex:IM-23964|pubmed:24963139 taxid:10090(mouse)|taxid:10090(Mus musculus) taxid:10090(mouse)|taxid:10090(Mus musculus) psi-mi:"MI:2364"(proximity) psi-mi:"MI:0469"(IntAct) intact:EBI-10683980|imex:IM-23964-19 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) interpro:IPR007110(Immunoglobulin-like)|interpro:IPR013783(Immunoglobulin-like fold)|interpro:IPR003006(Immunoglobulin/major histocompatibility complex, conserved site)|interpro:IPR003597(Immunoglobulin C1-set)|go:"GO:0005886"(plasma membrane)|go:"GO:0016021"(integral component of membrane)|interpro:IPR036179 refseq:NP_038734.1|ensembl:ENSMUSG00000000682(gene)|ensembl:ENSMUST00000000696(transcript)|go:"GO:0005886"(plasma membrane)|go:"GO:0007204"(positive regulation of cytosolic calcium ion concentration)|go:"GO:0009617"(response to bacterium)|go:"GO:0031225"(anchored component of membrane)|go:"GO:0097225"(sperm midpiece)|interpro:IPR026643|reactome:R-MMU-163125 - - - figure legend:Figure 7 Supplementary 2|comment:"There is slight loss of Fab-PLA signal upon exposure of B cells to antigen (NIP)."|antagonist:Specific antigen- NIP|dataset:Affinomics - Interactions curated for the Affinomics consortium.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:10090(mouse-tko-pre-b-cells)|taxid:10090("Mouse Triple_ Knock_out pre-B cells:SLP-65,Rag2, CD179b deficient") - 2015/02/13 2015/02/24 rogid:WPb0XrDhxuYDQ0jFtnq2QDd6u1c10090 rogid:QKz1onjxDperltn/dTei86qYFGo10090 intact-crc:ECE38B6493FCAABF|rigid:0YSYfap1tvIF9lDXOFKNVRHexpc false - - - - psi-mi:"MI:0833"(autoradiography) psi-mi:"MI:0833"(autoradiography) uniprotkb:P25918 uniprotkb:P01882 intact:EBI-644463|uniprotkb:Q542B2|ensembl:ENSMUSP00000145803 intact:EBI-10683684 psi-mi:cd19_mouse(display_long)|uniprotkb:Differentiation antigen CD19(gene name synonym)|uniprotkb:Cd19(gene name)|psi-mi:Cd19(display_short) psi-mi:ighdm_mouse(display_long) psi-mi:"MI:0813"(proximity ligation assay) Kläsener et al. (2014) imex:IM-23964|pubmed:24963139 taxid:10090(mouse)|taxid:10090(Mus musculus) taxid:10090(mouse)|taxid:10090(Mus musculus) psi-mi:"MI:2364"(proximity) psi-mi:"MI:0469"(IntAct) intact:EBI-10683926|imex:IM-23964-59 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) refseq:NP_033974.2|ensembl:ENSMUSG00000030724(gene)|ensembl:ENSMUST00000206325(transcript)|go:"GO:0001923"(B-1 B cell differentiation)|go:"GO:0002322"(B cell proliferation involved in immune response)|go:"GO:0005886"(plasma membrane)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0009897"(external side of plasma membrane)|go:"GO:0016064"(immunoglobulin mediated immune response)|go:"GO:0019724"(B cell mediated immunity)|go:"GO:0032991"(protein-containing complex)|go:"GO:0043552"(positive regulation of phosphatidylinositol 3-kinase activity)|go:"GO:0045121"(membrane raft)|go:"GO:0050851"(antigen receptor-mediated signaling pathway)|go:"GO:0050853"(B cell receptor signaling pathway)|go:"GO:0050855"(regulation of B cell receptor signaling pathway)|go:"GO:0050864"(regulation of B cell activation)|go:"GO:0051281"(positive regulation of release of sequestered calcium ion into cytosol)|interpro:IPR003599(Immunoglobulin subtype)|interpro:IPR007110(Immunoglobulin-like)|interpro:IPR013783(Immunoglobulin-like fold)|interpro:IPR036179|interpro:IPR042341|reactome:R-MMU-1257604|reactome:R-MMU-198933|reactome:R-MMU-6811558|reactome:R-MMU-977606|reactome:R-MMU-983695|refseq:XP_006507346.1 interpro:IPR007110(Immunoglobulin-like)|interpro:IPR013783(Immunoglobulin-like fold)|interpro:IPR003006(Immunoglobulin/major histocompatibility complex, conserved site)|interpro:IPR003597(Immunoglobulin C1-set)|go:"GO:0005886"(plasma membrane)|go:"GO:0016021"(integral component of membrane)|interpro:IPR036179 - - - figure legend:Figure 7E Supplementary 1|comment:"Resting TKO-D cells display a close proximity between the IgD-BCR and the CD19/CD20 module but lose this interaction upon BCR stimulation The Fab-PLA signal is lost upon exposure of B cells to antigen (NIP), or in presence of BCR-activating drugs."|antagonist:Specific antigen- NIP, pervanadate and Lat-A - activators of the BCR|dataset:Affinomics - Interactions curated for the Affinomics consortium.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:10090(mouse-tko-pre-b-cells)|taxid:10090("Mouse Triple_ Knock_out pre-B cells:SLP-65,Rag2, CD179b deficient") - 2015/02/13 2015/03/05 rogid:YWZCIrT3MOdFhZd5+gTZhMkF8yc10090 rogid:WPb0XrDhxuYDQ0jFtnq2QDd6u1c10090 intact-crc:3C146856B36616AC|rigid:rUMykdLzTsMp4bvtu6Aylx21hIQ false - - - - psi-mi:"MI:0833"(autoradiography) psi-mi:"MI:0833"(autoradiography) uniprotkb:P01882 uniprotkb:P19437 intact:EBI-10683684 intact:EBI-10683749|ensembl:ENSMUSP00000126422 psi-mi:ighdm_mouse(display_long) psi-mi:cd20_mouse(display_long)|uniprotkb:Ms4a1(gene name)|psi-mi:Ms4a1(display_short)|uniprotkb:Cd20(gene name synonym)|uniprotkb:Ly-44(gene name synonym)|uniprotkb:Ms4a2(gene name synonym)|uniprotkb:B-cell differentiation antigen Ly-44(gene name synonym)|uniprotkb:Lymphocyte antigen 44(gene name synonym)|uniprotkb:Membrane-spanning 4-domains subfamily A member 1(gene name synonym) psi-mi:"MI:0813"(proximity ligation assay) Kläsener et al. (2014) imex:IM-23964|pubmed:24963139 taxid:10090(mouse)|taxid:10090(Mus musculus) taxid:10090(mouse)|taxid:10090(Mus musculus) psi-mi:"MI:2364"(proximity) psi-mi:"MI:0469"(IntAct) intact:EBI-10683789|imex:IM-23964-11 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) interpro:IPR007110(Immunoglobulin-like)|interpro:IPR013783(Immunoglobulin-like fold)|interpro:IPR003006(Immunoglobulin/major histocompatibility complex, conserved site)|interpro:IPR003597(Immunoglobulin C1-set)|go:"GO:0005886"(plasma membrane)|go:"GO:0016021"(integral component of membrane)|interpro:IPR036179 refseq:NP_031667.1|ensembl:ENSMUSG00000024673(gene)|ensembl:ENSMUST00000169159(transcript)|go:"GO:0002115"(store-operated calcium entry)|go:"GO:0005154"(epidermal growth factor receptor binding)|go:"GO:0005654"(nucleoplasm)|go:"GO:0005886"(plasma membrane)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0009617"(response to bacterium)|go:"GO:0009897"(external side of plasma membrane)|go:"GO:0009986"(cell surface)|go:"GO:0019865"(immunoglobulin binding)|go:"GO:0030183"(B cell differentiation)|go:"GO:0042113"(B cell activation)|go:"GO:0042802"(identical protein binding)|go:"GO:0044853"(plasma membrane raft)|go:"GO:0050853"(B cell receptor signaling pathway)|go:"GO:0051262"(protein tetramerization)|go:"GO:1902656"(calcium ion import into cytosol)|interpro:IPR007237(CD20/IgE Fc receptor beta subunit)|interpro:IPR030417|interpro:IPR030418 - - - figure legend:7B-2|comment:CD19 and 20 are found together with the resting IgD-BCR and dissociate from this receptor upon BCR activation|antagonist:Specific antigen- NIP|dataset:Affinomics - Interactions curated for the Affinomics consortium.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:10090(mouse-tko-md-pre-b cells)|taxid:10090("Mouse TKO_MD Pre B Cells:KO for SLP-65, RAG2, CD179b, transfected with NOP specific IgM/IgD BCR") - 2015/02/13 2015/03/05 rogid:WPb0XrDhxuYDQ0jFtnq2QDd6u1c10090 rogid:GPiXJuTEbnfqoUhQ0apzTyBcbzg10090 intact-crc:DB2C3C205B9C0AF1|rigid:+ZdVbKS/sTYEGBe3RKOlZyer88E false - - - - psi-mi:"MI:0421"(identification by antibody) psi-mi:"MI:0421"(identification by antibody) uniprotkb:P01872 uniprotkb:P01872 intact:EBI-645833|uniprotkb:P01873 intact:EBI-645833|uniprotkb:P01873 psi-mi:ighm_mouse(display_long)|uniprotkb:Ighm(gene name)|psi-mi:Ighm(display_short)|uniprotkb:Igh-6(gene name synonym) psi-mi:ighm_mouse(display_long)|uniprotkb:Ighm(gene name)|psi-mi:Ighm(display_short)|uniprotkb:Igh-6(gene name synonym) psi-mi:"MI:0813"(proximity ligation assay) Kläsener et al. (2014) imex:IM-23964|pubmed:24963139 taxid:10090(mouse)|taxid:10090(Mus musculus) taxid:10090(mouse)|taxid:10090(Mus musculus) psi-mi:"MI:2364"(proximity) psi-mi:"MI:0469"(IntAct) intact:EBI-10683310|imex:IM-23964-41 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) interpro:IPR007110(Immunoglobulin-like)|interpro:IPR013783(Immunoglobulin-like fold)|interpro:IPR003006(Immunoglobulin/major histocompatibility complex, conserved site)|interpro:IPR003597(Immunoglobulin C1-set)|go:"GO:0002344"(B cell affinity maturation)|go:"GO:0002455"(humoral immune response mediated by circulating immunoglobulin)|go:"GO:0002637"(regulation of immunoglobulin production)|go:"GO:0003823"(antigen binding)|go:"GO:0004888"(transmembrane signaling receptor activity)|go:"GO:0005615"(extracellular space)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0006910"(phagocytosis, recognition)|go:"GO:0006911"(phagocytosis, engulfment)|go:"GO:0006958"(complement activation, classical pathway)|go:"GO:0009897"(external side of plasma membrane)|go:"GO:0009986"(cell surface)|go:"GO:0016020"(membrane)|go:"GO:0016021"(integral component of membrane)|go:"GO:0016064"(immunoglobulin mediated immune response)|go:"GO:0019815"(B cell receptor complex)|go:"GO:0019882"(antigen processing and presentation)|go:"GO:0022604"(regulation of cell morphogenesis)|go:"GO:0030890"(positive regulation of B cell proliferation)|go:"GO:0034987"(immunoglobulin receptor binding)|go:"GO:0042571"(immunoglobulin complex, circulating)|go:"GO:0042742"(defense response to bacterium)|go:"GO:0042802"(identical protein binding)|go:"GO:0043410"(positive regulation of MAPK cascade)|go:"GO:0045022"(early endosome to late endosome transport)|go:"GO:0045087"(innate immune response)|go:"GO:0045807"(positive regulation of endocytosis)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0050731"(positive regulation of peptidyl-tyrosine phosphorylation)|go:"GO:0050778"(positive regulation of immune response)|go:"GO:0050829"(defense response to Gram-negative bacterium)|go:"GO:0050853"(B cell receptor signaling pathway)|go:"GO:0050871"(positive regulation of B cell activation)|rcsb pdb:4BA8|rcsb pdb:4JVU|rcsb pdb:4JVW|reactome:R-MMU-202733|reactome:R-MMU-5690714|reactome:R-MMU-983695|interpro:IPR036179|mint:P01872 interpro:IPR007110(Immunoglobulin-like)|interpro:IPR013783(Immunoglobulin-like fold)|interpro:IPR003006(Immunoglobulin/major histocompatibility complex, conserved site)|interpro:IPR003597(Immunoglobulin C1-set)|go:"GO:0002344"(B cell affinity maturation)|go:"GO:0002455"(humoral immune response mediated by circulating immunoglobulin)|go:"GO:0002637"(regulation of immunoglobulin production)|go:"GO:0003823"(antigen binding)|go:"GO:0004888"(transmembrane signaling receptor activity)|go:"GO:0005615"(extracellular space)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0006910"(phagocytosis, recognition)|go:"GO:0006911"(phagocytosis, engulfment)|go:"GO:0006958"(complement activation, classical pathway)|go:"GO:0009897"(external side of plasma membrane)|go:"GO:0009986"(cell surface)|go:"GO:0016020"(membrane)|go:"GO:0016021"(integral component of membrane)|go:"GO:0016064"(immunoglobulin mediated immune response)|go:"GO:0019815"(B cell receptor complex)|go:"GO:0019882"(antigen processing and presentation)|go:"GO:0022604"(regulation of cell morphogenesis)|go:"GO:0030890"(positive regulation of B cell proliferation)|go:"GO:0034987"(immunoglobulin receptor binding)|go:"GO:0042571"(immunoglobulin complex, circulating)|go:"GO:0042742"(defense response to bacterium)|go:"GO:0042802"(identical protein binding)|go:"GO:0043410"(positive regulation of MAPK cascade)|go:"GO:0045022"(early endosome to late endosome transport)|go:"GO:0045087"(innate immune response)|go:"GO:0045807"(positive regulation of endocytosis)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0050731"(positive regulation of peptidyl-tyrosine phosphorylation)|go:"GO:0050778"(positive regulation of immune response)|go:"GO:0050829"(defense response to Gram-negative bacterium)|go:"GO:0050853"(B cell receptor signaling pathway)|go:"GO:0050871"(positive regulation of B cell activation)|rcsb pdb:4BA8|rcsb pdb:4JVU|rcsb pdb:4JVW|reactome:R-MMU-202733|reactome:R-MMU-5690714|reactome:R-MMU-983695|interpro:IPR036179|mint:P01872 - - - figure legend:1F/H, 4B|comment:"The Fab-PLA signal is lost upon exposure of B cells to antigen (NIP), or in presence of BCR-activating drugs. Only Fab-PLA showed a clear difference in the relative IgM:IgM conformation or distance between resting and activated B cells."|antagonist:Specific antigen- NIP, pervanadate and Lat-A.|dataset:Affinomics - Interactions curated for the Affinomics consortium.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:10090(mouse-tko-md-pre-b cells)|taxid:10090("Mouse TKO_MD Pre B Cells:KO for SLP-65, RAG2, CD179b, transfected with NOP specific IgM/IgD BCR") - 2015/02/13 2015/02/24 rogid:4+PnUlhyUfPZS43gGZMtyePhlCw10090 rogid:4+PnUlhyUfPZS43gGZMtyePhlCw10090 intact-crc:9ADF7D1C704CD42E|rigid:N/cK5ET0O9C0NgHLucUfovmg9nc false - - - - psi-mi:"MI:0421"(identification by antibody) psi-mi:"MI:0421"(identification by antibody) uniprotkb:P01872 uniprotkb:P25918 intact:EBI-645833|uniprotkb:P01873 intact:EBI-644463|uniprotkb:Q542B2|ensembl:ENSMUSP00000145803 psi-mi:ighm_mouse(display_long)|uniprotkb:Ighm(gene name)|psi-mi:Ighm(display_short)|uniprotkb:Igh-6(gene name synonym) psi-mi:cd19_mouse(display_long)|uniprotkb:Differentiation antigen CD19(gene name synonym)|uniprotkb:Cd19(gene name)|psi-mi:Cd19(display_short) psi-mi:"MI:0813"(proximity ligation assay) Kläsener et al. (2014) imex:IM-23964|pubmed:24963139 taxid:10090(mouse)|taxid:10090(Mus musculus) taxid:10090(mouse)|taxid:10090(Mus musculus) psi-mi:"MI:2364"(proximity) psi-mi:"MI:0469"(IntAct) intact:EBI-10683737|imex:IM-23964-65 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) interpro:IPR007110(Immunoglobulin-like)|interpro:IPR013783(Immunoglobulin-like fold)|interpro:IPR003006(Immunoglobulin/major histocompatibility complex, conserved site)|interpro:IPR003597(Immunoglobulin C1-set)|go:"GO:0002344"(B cell affinity maturation)|go:"GO:0002455"(humoral immune response mediated by circulating immunoglobulin)|go:"GO:0002637"(regulation of immunoglobulin production)|go:"GO:0003823"(antigen binding)|go:"GO:0004888"(transmembrane signaling receptor activity)|go:"GO:0005615"(extracellular space)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0006910"(phagocytosis, recognition)|go:"GO:0006911"(phagocytosis, engulfment)|go:"GO:0006958"(complement activation, classical pathway)|go:"GO:0009897"(external side of plasma membrane)|go:"GO:0009986"(cell surface)|go:"GO:0016020"(membrane)|go:"GO:0016021"(integral component of membrane)|go:"GO:0016064"(immunoglobulin mediated immune response)|go:"GO:0019815"(B cell receptor complex)|go:"GO:0019882"(antigen processing and presentation)|go:"GO:0022604"(regulation of cell morphogenesis)|go:"GO:0030890"(positive regulation of B cell proliferation)|go:"GO:0034987"(immunoglobulin receptor binding)|go:"GO:0042571"(immunoglobulin complex, circulating)|go:"GO:0042742"(defense response to bacterium)|go:"GO:0042802"(identical protein binding)|go:"GO:0043410"(positive regulation of MAPK cascade)|go:"GO:0045022"(early endosome to late endosome transport)|go:"GO:0045087"(innate immune response)|go:"GO:0045807"(positive regulation of endocytosis)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0050731"(positive regulation of peptidyl-tyrosine phosphorylation)|go:"GO:0050778"(positive regulation of immune response)|go:"GO:0050829"(defense response to Gram-negative bacterium)|go:"GO:0050853"(B cell receptor signaling pathway)|go:"GO:0050871"(positive regulation of B cell activation)|rcsb pdb:4BA8|rcsb pdb:4JVU|rcsb pdb:4JVW|reactome:R-MMU-202733|reactome:R-MMU-5690714|reactome:R-MMU-983695|interpro:IPR036179|mint:P01872 refseq:NP_033974.2|ensembl:ENSMUSG00000030724(gene)|ensembl:ENSMUST00000206325(transcript)|go:"GO:0001923"(B-1 B cell differentiation)|go:"GO:0002322"(B cell proliferation involved in immune response)|go:"GO:0005886"(plasma membrane)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0009897"(external side of plasma membrane)|go:"GO:0016064"(immunoglobulin mediated immune response)|go:"GO:0019724"(B cell mediated immunity)|go:"GO:0032991"(protein-containing complex)|go:"GO:0043552"(positive regulation of phosphatidylinositol 3-kinase activity)|go:"GO:0045121"(membrane raft)|go:"GO:0050851"(antigen receptor-mediated signaling pathway)|go:"GO:0050853"(B cell receptor signaling pathway)|go:"GO:0050855"(regulation of B cell receptor signaling pathway)|go:"GO:0050864"(regulation of B cell activation)|go:"GO:0051281"(positive regulation of release of sequestered calcium ion into cytosol)|interpro:IPR003599(Immunoglobulin subtype)|interpro:IPR007110(Immunoglobulin-like)|interpro:IPR013783(Immunoglobulin-like fold)|interpro:IPR036179|interpro:IPR042341|reactome:R-MMU-1257604|reactome:R-MMU-198933|reactome:R-MMU-6811558|reactome:R-MMU-977606|reactome:R-MMU-983695|refseq:XP_006507346.1 - - - figure legend:7A|comment:CD19 is only found in close association with the activated but not with the resting IgM-BCR.|agonist:Specific antigen- NIP|dataset:Affinomics - Interactions curated for the Affinomics consortium.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:10090(mouse-tko-md-pre-b cells)|taxid:10090("Mouse TKO_MD Pre B Cells:KO for SLP-65, RAG2, CD179b, transfected with NOP specific IgM/IgD BCR") - 2015/02/13 2015/03/05 rogid:4+PnUlhyUfPZS43gGZMtyePhlCw10090 rogid:YWZCIrT3MOdFhZd5+gTZhMkF8yc10090 intact-crc:44BEFD7A5DBAA877|rigid:A/Bc70lm3EejkcIeAaEQImf+Ntg false - - - - psi-mi:"MI:0421"(identification by antibody) psi-mi:"MI:0421"(identification by antibody) uniprotkb:P25918 uniprotkb:P01882 intact:EBI-644463|uniprotkb:Q542B2|ensembl:ENSMUSP00000145803 intact:EBI-10683684 psi-mi:cd19_mouse(display_long)|uniprotkb:Differentiation antigen CD19(gene name synonym)|uniprotkb:Cd19(gene name)|psi-mi:Cd19(display_short) psi-mi:ighdm_mouse(display_long) psi-mi:"MI:0813"(proximity ligation assay) Kläsener et al. (2014) imex:IM-23964|pubmed:24963139 taxid:10090(mouse)|taxid:10090(Mus musculus) taxid:10090(mouse)|taxid:10090(Mus musculus) psi-mi:"MI:2364"(proximity) psi-mi:"MI:0469"(IntAct) intact:EBI-10683783|imex:IM-23964-51 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) refseq:NP_033974.2|ensembl:ENSMUSG00000030724(gene)|ensembl:ENSMUST00000206325(transcript)|go:"GO:0001923"(B-1 B cell differentiation)|go:"GO:0002322"(B cell proliferation involved in immune response)|go:"GO:0005886"(plasma membrane)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0009897"(external side of plasma membrane)|go:"GO:0016064"(immunoglobulin mediated immune response)|go:"GO:0019724"(B cell mediated immunity)|go:"GO:0032991"(protein-containing complex)|go:"GO:0043552"(positive regulation of phosphatidylinositol 3-kinase activity)|go:"GO:0045121"(membrane raft)|go:"GO:0050851"(antigen receptor-mediated signaling pathway)|go:"GO:0050853"(B cell receptor signaling pathway)|go:"GO:0050855"(regulation of B cell receptor signaling pathway)|go:"GO:0050864"(regulation of B cell activation)|go:"GO:0051281"(positive regulation of release of sequestered calcium ion into cytosol)|interpro:IPR003599(Immunoglobulin subtype)|interpro:IPR007110(Immunoglobulin-like)|interpro:IPR013783(Immunoglobulin-like fold)|interpro:IPR036179|interpro:IPR042341|reactome:R-MMU-1257604|reactome:R-MMU-198933|reactome:R-MMU-6811558|reactome:R-MMU-977606|reactome:R-MMU-983695|refseq:XP_006507346.1 interpro:IPR007110(Immunoglobulin-like)|interpro:IPR013783(Immunoglobulin-like fold)|interpro:IPR003006(Immunoglobulin/major histocompatibility complex, conserved site)|interpro:IPR003597(Immunoglobulin C1-set)|go:"GO:0005886"(plasma membrane)|go:"GO:0016021"(integral component of membrane)|interpro:IPR036179 - - - figure legend:7B|comment:CD19 and 20 are found together with the resting IgD-BCR and dissociate from this receptor upon BCR activation|antagonist:Specific antigen- NIP|dataset:Affinomics - Interactions curated for the Affinomics consortium.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:10090(mouse-tko-md-pre-b cells)|taxid:10090("Mouse TKO_MD Pre B Cells:KO for SLP-65, RAG2, CD179b, transfected with NOP specific IgM/IgD BCR") - 2015/02/13 2015/02/24 rogid:YWZCIrT3MOdFhZd5+gTZhMkF8yc10090 rogid:WPb0XrDhxuYDQ0jFtnq2QDd6u1c10090 intact-crc:05AAACFFA96640CD|rigid:rUMykdLzTsMp4bvtu6Aylx21hIQ false - - - - psi-mi:"MI:0421"(identification by antibody) psi-mi:"MI:0421"(identification by antibody) uniprotkb:P19437 uniprotkb:P01872 intact:EBI-10683749|ensembl:ENSMUSP00000126422 intact:EBI-645833|uniprotkb:P01873 psi-mi:cd20_mouse(display_long)|uniprotkb:Ms4a1(gene name)|psi-mi:Ms4a1(display_short)|uniprotkb:Cd20(gene name synonym)|uniprotkb:Ly-44(gene name synonym)|uniprotkb:Ms4a2(gene name synonym)|uniprotkb:B-cell differentiation antigen Ly-44(gene name synonym)|uniprotkb:Lymphocyte antigen 44(gene name synonym)|uniprotkb:Membrane-spanning 4-domains subfamily A member 1(gene name synonym) psi-mi:ighm_mouse(display_long)|uniprotkb:Ighm(gene name)|psi-mi:Ighm(display_short)|uniprotkb:Igh-6(gene name synonym) psi-mi:"MI:0813"(proximity ligation assay) Kläsener et al. (2014) imex:IM-23964|pubmed:24963139 taxid:10090(mouse)|taxid:10090(Mus musculus) taxid:10090(mouse)|taxid:10090(Mus musculus) psi-mi:"MI:2364"(proximity) psi-mi:"MI:0469"(IntAct) intact:EBI-10683743|imex:IM-23964-27 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) refseq:NP_031667.1|ensembl:ENSMUSG00000024673(gene)|ensembl:ENSMUST00000169159(transcript)|go:"GO:0002115"(store-operated calcium entry)|go:"GO:0005154"(epidermal growth factor receptor binding)|go:"GO:0005654"(nucleoplasm)|go:"GO:0005886"(plasma membrane)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0009617"(response to bacterium)|go:"GO:0009897"(external side of plasma membrane)|go:"GO:0009986"(cell surface)|go:"GO:0019865"(immunoglobulin binding)|go:"GO:0030183"(B cell differentiation)|go:"GO:0042113"(B cell activation)|go:"GO:0042802"(identical protein binding)|go:"GO:0044853"(plasma membrane raft)|go:"GO:0050853"(B cell receptor signaling pathway)|go:"GO:0051262"(protein tetramerization)|go:"GO:1902656"(calcium ion import into cytosol)|interpro:IPR007237(CD20/IgE Fc receptor beta subunit)|interpro:IPR030417|interpro:IPR030418 interpro:IPR007110(Immunoglobulin-like)|interpro:IPR013783(Immunoglobulin-like fold)|interpro:IPR003006(Immunoglobulin/major histocompatibility complex, conserved site)|interpro:IPR003597(Immunoglobulin C1-set)|go:"GO:0002344"(B cell affinity maturation)|go:"GO:0002455"(humoral immune response mediated by circulating immunoglobulin)|go:"GO:0002637"(regulation of immunoglobulin production)|go:"GO:0003823"(antigen binding)|go:"GO:0004888"(transmembrane signaling receptor activity)|go:"GO:0005615"(extracellular space)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0006910"(phagocytosis, recognition)|go:"GO:0006911"(phagocytosis, engulfment)|go:"GO:0006958"(complement activation, classical pathway)|go:"GO:0009897"(external side of plasma membrane)|go:"GO:0009986"(cell surface)|go:"GO:0016020"(membrane)|go:"GO:0016021"(integral component of membrane)|go:"GO:0016064"(immunoglobulin mediated immune response)|go:"GO:0019815"(B cell receptor complex)|go:"GO:0019882"(antigen processing and presentation)|go:"GO:0022604"(regulation of cell morphogenesis)|go:"GO:0030890"(positive regulation of B cell proliferation)|go:"GO:0034987"(immunoglobulin receptor binding)|go:"GO:0042571"(immunoglobulin complex, circulating)|go:"GO:0042742"(defense response to bacterium)|go:"GO:0042802"(identical protein binding)|go:"GO:0043410"(positive regulation of MAPK cascade)|go:"GO:0045022"(early endosome to late endosome transport)|go:"GO:0045087"(innate immune response)|go:"GO:0045807"(positive regulation of endocytosis)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0050731"(positive regulation of peptidyl-tyrosine phosphorylation)|go:"GO:0050778"(positive regulation of immune response)|go:"GO:0050829"(defense response to Gram-negative bacterium)|go:"GO:0050853"(B cell receptor signaling pathway)|go:"GO:0050871"(positive regulation of B cell activation)|rcsb pdb:4BA8|rcsb pdb:4JVU|rcsb pdb:4JVW|reactome:R-MMU-202733|reactome:R-MMU-5690714|reactome:R-MMU-983695|interpro:IPR036179|mint:P01872 - - - figure legend:7A-2|comment:CD20 is only found in close association with the activated but not with the resting IgM-BCR.|agonist:Specific antigen- NIP|dataset:Affinomics - Interactions curated for the Affinomics consortium.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:10090(mouse-tko-md-pre-b cells)|taxid:10090("Mouse TKO_MD Pre B Cells:KO for SLP-65, RAG2, CD179b, transfected with NOP specific IgM/IgD BCR") - 2015/02/13 2015/02/24 rogid:GPiXJuTEbnfqoUhQ0apzTyBcbzg10090 rogid:4+PnUlhyUfPZS43gGZMtyePhlCw10090 intact-crc:BFA7A782546EE787|rigid:eOiDaeqdonr2Q7LU76dmHoQK5I0 false - - - - psi-mi:"MI:0421"(identification by antibody) psi-mi:"MI:0421"(identification by antibody) uniprotkb:P01882 uniprotkb:P01882 intact:EBI-10683684 intact:EBI-10683684 psi-mi:ighdm_mouse(display_long) psi-mi:ighdm_mouse(display_long) psi-mi:"MI:0813"(proximity ligation assay) Kläsener et al. (2014) imex:IM-23964|pubmed:24963139 taxid:10090(mouse)|taxid:10090(Mus musculus) taxid:10090(mouse)|taxid:10090(Mus musculus) psi-mi:"MI:2364"(proximity) psi-mi:"MI:0469"(IntAct) intact:EBI-10683706|imex:IM-23964-3 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) interpro:IPR007110(Immunoglobulin-like)|interpro:IPR013783(Immunoglobulin-like fold)|interpro:IPR003006(Immunoglobulin/major histocompatibility complex, conserved site)|interpro:IPR003597(Immunoglobulin C1-set)|go:"GO:0005886"(plasma membrane)|go:"GO:0016021"(integral component of membrane)|interpro:IPR036179 interpro:IPR007110(Immunoglobulin-like)|interpro:IPR013783(Immunoglobulin-like fold)|interpro:IPR003006(Immunoglobulin/major histocompatibility complex, conserved site)|interpro:IPR003597(Immunoglobulin C1-set)|go:"GO:0005886"(plasma membrane)|go:"GO:0016021"(integral component of membrane)|interpro:IPR036179 - - - figure legend:4B|comment:The Fab-PLA signal is lost upon exposure of B cells to antigen, or in presence of BCR-activating drugs.|antagonist:"specific antigen-NIP, pervanadate (Perv) and Lat-A - activators of the BCR."|dataset:Affinomics - Interactions curated for the Affinomics consortium.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:10090(mouse-tko-md-pre-b cells)|taxid:10090("Mouse TKO_MD Pre B Cells:KO for SLP-65, RAG2, CD179b, transfected with NOP specific IgM/IgD BCR") - 2015/02/13 2015/03/05 rogid:WPb0XrDhxuYDQ0jFtnq2QDd6u1c10090 rogid:WPb0XrDhxuYDQ0jFtnq2QDd6u1c10090 intact-crc:6BD9A2106EE34882|rigid:Kbyvm7r3c+tkujEdaJCVrV1B61A false - - - - psi-mi:"MI:0421"(identification by antibody) psi-mi:"MI:0421"(identification by antibody) uniprotkb:P01872 uniprotkb:P01872 intact:EBI-645833|uniprotkb:P01873 intact:EBI-645833|uniprotkb:P01873 psi-mi:ighm_mouse(display_long)|uniprotkb:Ighm(gene name)|psi-mi:Ighm(display_short)|uniprotkb:Igh-6(gene name synonym) psi-mi:ighm_mouse(display_long)|uniprotkb:Ighm(gene name)|psi-mi:Ighm(display_short)|uniprotkb:Igh-6(gene name synonym) psi-mi:"MI:0054"(fluorescence-activated cell sorting) Kläsener et al. (2014) imex:IM-23964|pubmed:24963139 taxid:10090(mouse)|taxid:10090(Mus musculus) taxid:10090(mouse)|taxid:10090(Mus musculus) psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-10683395|imex:IM-23964-25 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) interpro:IPR007110(Immunoglobulin-like)|interpro:IPR013783(Immunoglobulin-like fold)|interpro:IPR003006(Immunoglobulin/major histocompatibility complex, conserved site)|interpro:IPR003597(Immunoglobulin C1-set)|go:"GO:0002344"(B cell affinity maturation)|go:"GO:0002455"(humoral immune response mediated by circulating immunoglobulin)|go:"GO:0002637"(regulation of immunoglobulin production)|go:"GO:0003823"(antigen binding)|go:"GO:0004888"(transmembrane signaling receptor activity)|go:"GO:0005615"(extracellular space)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0006910"(phagocytosis, recognition)|go:"GO:0006911"(phagocytosis, engulfment)|go:"GO:0006958"(complement activation, classical pathway)|go:"GO:0009897"(external side of plasma membrane)|go:"GO:0009986"(cell surface)|go:"GO:0016020"(membrane)|go:"GO:0016021"(integral component of membrane)|go:"GO:0016064"(immunoglobulin mediated immune response)|go:"GO:0019815"(B cell receptor complex)|go:"GO:0019882"(antigen processing and presentation)|go:"GO:0022604"(regulation of cell morphogenesis)|go:"GO:0030890"(positive regulation of B cell proliferation)|go:"GO:0034987"(immunoglobulin receptor binding)|go:"GO:0042571"(immunoglobulin complex, circulating)|go:"GO:0042742"(defense response to bacterium)|go:"GO:0042802"(identical protein binding)|go:"GO:0043410"(positive regulation of MAPK cascade)|go:"GO:0045022"(early endosome to late endosome transport)|go:"GO:0045087"(innate immune response)|go:"GO:0045807"(positive regulation of endocytosis)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0050731"(positive regulation of peptidyl-tyrosine phosphorylation)|go:"GO:0050778"(positive regulation of immune response)|go:"GO:0050829"(defense response to Gram-negative bacterium)|go:"GO:0050853"(B cell receptor signaling pathway)|go:"GO:0050871"(positive regulation of B cell activation)|rcsb pdb:4BA8|rcsb pdb:4JVU|rcsb pdb:4JVW|reactome:R-MMU-202733|reactome:R-MMU-5690714|reactome:R-MMU-983695|interpro:IPR036179|mint:P01872 interpro:IPR007110(Immunoglobulin-like)|interpro:IPR013783(Immunoglobulin-like fold)|interpro:IPR003006(Immunoglobulin/major histocompatibility complex, conserved site)|interpro:IPR003597(Immunoglobulin C1-set)|go:"GO:0002344"(B cell affinity maturation)|go:"GO:0002455"(humoral immune response mediated by circulating immunoglobulin)|go:"GO:0002637"(regulation of immunoglobulin production)|go:"GO:0003823"(antigen binding)|go:"GO:0004888"(transmembrane signaling receptor activity)|go:"GO:0005615"(extracellular space)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0006910"(phagocytosis, recognition)|go:"GO:0006911"(phagocytosis, engulfment)|go:"GO:0006958"(complement activation, classical pathway)|go:"GO:0009897"(external side of plasma membrane)|go:"GO:0009986"(cell surface)|go:"GO:0016020"(membrane)|go:"GO:0016021"(integral component of membrane)|go:"GO:0016064"(immunoglobulin mediated immune response)|go:"GO:0019815"(B cell receptor complex)|go:"GO:0019882"(antigen processing and presentation)|go:"GO:0022604"(regulation of cell morphogenesis)|go:"GO:0030890"(positive regulation of B cell proliferation)|go:"GO:0034987"(immunoglobulin receptor binding)|go:"GO:0042571"(immunoglobulin complex, circulating)|go:"GO:0042742"(defense response to bacterium)|go:"GO:0042802"(identical protein binding)|go:"GO:0043410"(positive regulation of MAPK cascade)|go:"GO:0045022"(early endosome to late endosome transport)|go:"GO:0045087"(innate immune response)|go:"GO:0045807"(positive regulation of endocytosis)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0050731"(positive regulation of peptidyl-tyrosine phosphorylation)|go:"GO:0050778"(positive regulation of immune response)|go:"GO:0050829"(defense response to Gram-negative bacterium)|go:"GO:0050853"(B cell receptor signaling pathway)|go:"GO:0050871"(positive regulation of B cell activation)|rcsb pdb:4BA8|rcsb pdb:4JVU|rcsb pdb:4JVW|reactome:R-MMU-202733|reactome:R-MMU-5690714|reactome:R-MMU-983695|interpro:IPR036179|mint:P01872 - - - figure legend:2C|comment:Two IgM monomers interact.|dataset:Affinomics - Interactions curated for the Affinomics consortium.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/02/13 2015/03/27 rogid:4+PnUlhyUfPZS43gGZMtyePhlCw10090 rogid:4+PnUlhyUfPZS43gGZMtyePhlCw10090 intact-crc:A83762E8C995C035|rigid:N/cK5ET0O9C0NgHLucUfovmg9nc false - - - - psi-mi:"MI:0396"(predetermined participant) psi-mi:"MI:0396"(predetermined participant) uniprotkb:P01872 uniprotkb:P01872 intact:EBI-645833|uniprotkb:P01873 intact:EBI-645833|uniprotkb:P01873 psi-mi:ighm_mouse(display_long)|uniprotkb:Ighm(gene name)|psi-mi:Ighm(display_short)|uniprotkb:Igh-6(gene name synonym) psi-mi:ighm_mouse(display_long)|uniprotkb:Ighm(gene name)|psi-mi:Ighm(display_short)|uniprotkb:Igh-6(gene name synonym) psi-mi:"MI:0054"(fluorescence-activated cell sorting) Kläsener et al. (2014) imex:IM-23964|pubmed:24963139 taxid:10090(mouse)|taxid:10090(Mus musculus) taxid:10090(mouse)|taxid:10090(Mus musculus) psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-10683398|imex:IM-23964-70 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) interpro:IPR007110(Immunoglobulin-like)|interpro:IPR013783(Immunoglobulin-like fold)|interpro:IPR003006(Immunoglobulin/major histocompatibility complex, conserved site)|interpro:IPR003597(Immunoglobulin C1-set)|go:"GO:0002344"(B cell affinity maturation)|go:"GO:0002455"(humoral immune response mediated by circulating immunoglobulin)|go:"GO:0002637"(regulation of immunoglobulin production)|go:"GO:0003823"(antigen binding)|go:"GO:0004888"(transmembrane signaling receptor activity)|go:"GO:0005615"(extracellular space)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0006910"(phagocytosis, recognition)|go:"GO:0006911"(phagocytosis, engulfment)|go:"GO:0006958"(complement activation, classical pathway)|go:"GO:0009897"(external side of plasma membrane)|go:"GO:0009986"(cell surface)|go:"GO:0016020"(membrane)|go:"GO:0016021"(integral component of membrane)|go:"GO:0016064"(immunoglobulin mediated immune response)|go:"GO:0019815"(B cell receptor complex)|go:"GO:0019882"(antigen processing and presentation)|go:"GO:0022604"(regulation of cell morphogenesis)|go:"GO:0030890"(positive regulation of B cell proliferation)|go:"GO:0034987"(immunoglobulin receptor binding)|go:"GO:0042571"(immunoglobulin complex, circulating)|go:"GO:0042742"(defense response to bacterium)|go:"GO:0042802"(identical protein binding)|go:"GO:0043410"(positive regulation of MAPK cascade)|go:"GO:0045022"(early endosome to late endosome transport)|go:"GO:0045087"(innate immune response)|go:"GO:0045807"(positive regulation of endocytosis)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0050731"(positive regulation of peptidyl-tyrosine phosphorylation)|go:"GO:0050778"(positive regulation of immune response)|go:"GO:0050829"(defense response to Gram-negative bacterium)|go:"GO:0050853"(B cell receptor signaling pathway)|go:"GO:0050871"(positive regulation of B cell activation)|rcsb pdb:4BA8|rcsb pdb:4JVU|rcsb pdb:4JVW|reactome:R-MMU-202733|reactome:R-MMU-5690714|reactome:R-MMU-983695|interpro:IPR036179|mint:P01872 interpro:IPR007110(Immunoglobulin-like)|interpro:IPR013783(Immunoglobulin-like fold)|interpro:IPR003006(Immunoglobulin/major histocompatibility complex, conserved site)|interpro:IPR003597(Immunoglobulin C1-set)|go:"GO:0002344"(B cell affinity maturation)|go:"GO:0002455"(humoral immune response mediated by circulating immunoglobulin)|go:"GO:0002637"(regulation of immunoglobulin production)|go:"GO:0003823"(antigen binding)|go:"GO:0004888"(transmembrane signaling receptor activity)|go:"GO:0005615"(extracellular space)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0006910"(phagocytosis, recognition)|go:"GO:0006911"(phagocytosis, engulfment)|go:"GO:0006958"(complement activation, classical pathway)|go:"GO:0009897"(external side of plasma membrane)|go:"GO:0009986"(cell surface)|go:"GO:0016020"(membrane)|go:"GO:0016021"(integral component of membrane)|go:"GO:0016064"(immunoglobulin mediated immune response)|go:"GO:0019815"(B cell receptor complex)|go:"GO:0019882"(antigen processing and presentation)|go:"GO:0022604"(regulation of cell morphogenesis)|go:"GO:0030890"(positive regulation of B cell proliferation)|go:"GO:0034987"(immunoglobulin receptor binding)|go:"GO:0042571"(immunoglobulin complex, circulating)|go:"GO:0042742"(defense response to bacterium)|go:"GO:0042802"(identical protein binding)|go:"GO:0043410"(positive regulation of MAPK cascade)|go:"GO:0045022"(early endosome to late endosome transport)|go:"GO:0045087"(innate immune response)|go:"GO:0045807"(positive regulation of endocytosis)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0050731"(positive regulation of peptidyl-tyrosine phosphorylation)|go:"GO:0050778"(positive regulation of immune response)|go:"GO:0050829"(defense response to Gram-negative bacterium)|go:"GO:0050853"(B cell receptor signaling pathway)|go:"GO:0050871"(positive regulation of B cell activation)|rcsb pdb:4BA8|rcsb pdb:4JVU|rcsb pdb:4JVW|reactome:R-MMU-202733|reactome:R-MMU-5690714|reactome:R-MMU-983695|interpro:IPR036179|mint:P01872 - - - figure legend:2C-2|comment:1-PLA detects both soluble forms of IgM equally well - monomers and pentamers.|dataset:Affinomics - Interactions curated for the Affinomics consortium.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/02/13 2015/04/07 rogid:4+PnUlhyUfPZS43gGZMtyePhlCw10090 rogid:4+PnUlhyUfPZS43gGZMtyePhlCw10090 intact-crc:6834DF365C1C016F|rigid:N/cK5ET0O9C0NgHLucUfovmg9nc false - - 5 5 psi-mi:"MI:0396"(predetermined participant) psi-mi:"MI:0396"(predetermined participant) uniprotkb:P15391 uniprotkb:P01871 intact:EBI-79902|uniprotkb:A0N0P9|ensembl:ENSP00000437940|uniprotkb:Q96S68|uniprotkb:Q9BRD6|uniprotkb:F5H635 intact:EBI-953797|intact:EBI-28974488|uniprotkb:P20769|uniprotkb:A0A075B6N9|uniprotkb:A0A0G2JQL4|uniprotkb:P04220|ensembl:ENSP00000375001|ensembl:ENSP00000485962 psi-mi:cd19_human(display_long)|uniprotkb:CD19(gene name)|psi-mi:CD19(display_short)|uniprotkb:Differentiation antigen CD19(gene name synonym)|uniprotkb:B-lymphocyte surface antigen B4(gene name synonym)|uniprotkb:T-cell surface antigen Leu-12(gene name synonym) psi-mi:ighm_human(display_long)|uniprotkb:Ig mu chain C region BOT(gene name synonym)|uniprotkb:IGHM(gene name)|psi-mi:IGHM(display_short)|uniprotkb:Ig mu chain C region(gene name synonym)|uniprotkb:Ig mu chain C region GAL(gene name synonym)|uniprotkb:Ig mu chain C region OU(gene name synonym) psi-mi:"MI:0813"(proximity ligation assay) Kläsener et al. (2014) imex:IM-23964|pubmed:24963139 taxid:9606(human)|taxid:9606(Homo sapiens) taxid:9606(human)|taxid:9606(Homo sapiens) psi-mi:"MI:2364"(proximity) psi-mi:"MI:0469"(IntAct) intact:EBI-10683777|imex:IM-23964-9 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) ensembl:ENSG00000177455(gene)|ensembl:ENST00000538922(transcript)|go:"GO:0001923"(B-1 B cell differentiation)|go:"GO:0002322"(B cell proliferation involved in immune response)|go:"GO:0005886"(plasma membrane)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0009897"(external side of plasma membrane)|go:"GO:0016064"(immunoglobulin mediated immune response)|go:"GO:0032991"(protein-containing complex)|go:"GO:0043552"(positive regulation of phosphatidylinositol 3-kinase activity)|go:"GO:0045121"(membrane raft)|go:"GO:0050851"(antigen receptor-mediated signaling pathway)|go:"GO:0050853"(B cell receptor signaling pathway)|go:"GO:0050855"(regulation of B cell receptor signaling pathway)|go:"GO:0050864"(regulation of B cell activation)|go:"GO:0051281"(positive regulation of release of sequestered calcium ion into cytosol)|go:"GO:0070062"(extracellular exosome)|interpro:IPR003599(Immunoglobulin subtype)|interpro:IPR007110(Immunoglobulin-like)|interpro:IPR013783(Immunoglobulin-like fold)|interpro:IPR036179|interpro:IPR042341|mint:P15391|rcsb pdb:6AL5|rcsb pdb:7JIC|reactome:R-HSA-1257604|reactome:R-HSA-198933|reactome:R-HSA-2219530|reactome:R-HSA-6811558|reactome:R-HSA-977606|reactome:R-HSA-983695|refseq:NP_001171569.1|refseq:NP_001761.3 ensembl:ENSP00000375001|ensembl:ENSP00000485962|ensembl:ENST00000637539|ensembl:ENSP00000490253|go:"GO:0009897"(external side of plasma membrane)|go:"GO:0009986"(cell surface)|go:"GO:0016021"(integral component of membrane)|go:"GO:0019731"(antibacterial humoral response)|go:"GO:0042571"(immunoglobulin complex, circulating)|go:"GO:0042742"(defense response to bacterium)|go:"GO:0045087"(innate immune response)|go:"GO:0050829"(defense response to Gram-negative bacterium)|go:"GO:0050853"(B cell receptor signaling pathway)|go:"GO:0050871"(positive regulation of B cell activation)|go:"GO:0070062"(extracellular exosome)|go:"GO:0071756"(pentameric IgM immunoglobulin complex)|go:"GO:0071757"(hexameric IgM immunoglobulin complex)|go:"GO:0072562"(blood microparticle)|interpro:IPR003006(Immunoglobulin/major histocompatibility complex, conserved site)|interpro:IPR003597(Immunoglobulin C1-set)|interpro:IPR007110(Immunoglobulin-like)|interpro:IPR013783(Immunoglobulin-like fold)|interpro:IPR036179|mint:P01871|rcsb pdb:1HEZ|rcsb pdb:2AGJ|rcsb pdb:2RCJ|rcsb pdb:6KXS|rcsb pdb:7K0C|reactome:R-HSA-202733|reactome:R-HSA-5690714|reactome:R-HSA-983695|ensembl:ENSG00000211899(gene)|ensembl:ENSG00000282657(gene)|ensembl:ENST00000390559(transcript)|ensembl:ENST00000626472(transcript)|go:"GO:0002250"(adaptive immune response)|go:"GO:0003823"(antigen binding)|go:"GO:0005615"(extracellular space)|go:"GO:0005886"(plasma membrane)|go:"GO:0006910"(phagocytosis, recognition)|go:"GO:0006911"(phagocytosis, engulfment)|go:"GO:0006958"(complement activation, classical pathway) - - - figure legend:7C|comment:CD19 is only found in close association with the activated but not with the resting IgM-BCR.|agonist:Specific antigen- NIP|dataset:Affinomics - Interactions curated for the Affinomics consortium.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:9606(human-b-cell)|taxid:9606(Homo sapiens B cell) - 2015/02/13 2017/03/30 rogid:RXBn8jYrGc1LM3POARtQeEzKWoI9606 rogid:UMG2n3hbO3ecgM7+WS7M/l5b/Eg9606 intact-crc:5B6A136D1CAEFA70|rigid:9dSRXU3/gEs1zioyQrj612Lkm8Q false - - - - psi-mi:"MI:0421"(identification by antibody) psi-mi:"MI:0421"(identification by antibody) uniprotkb:P15391 uniprotkb:P01880 intact:EBI-79902|uniprotkb:A0N0P9|ensembl:ENSP00000437940|uniprotkb:Q96S68|uniprotkb:Q9BRD6|uniprotkb:F5H635 intact:EBI-10683716|uniprotkb:A0A087WUS7|uniprotkb:Q6P4I8|uniprotkb:Q8WU38 psi-mi:cd19_human(display_long)|uniprotkb:CD19(gene name)|psi-mi:CD19(display_short)|uniprotkb:Differentiation antigen CD19(gene name synonym)|uniprotkb:B-lymphocyte surface antigen B4(gene name synonym)|uniprotkb:T-cell surface antigen Leu-12(gene name synonym) psi-mi:ighd_human(display_long)|uniprotkb:IGHD(gene name)|psi-mi:IGHD(display_short)|uniprotkb:Ig delta chain C region(gene name synonym)|uniprotkb:Ig delta chain C region NIG-65(gene name synonym)|uniprotkb:Ig delta chain C region WAH(gene name synonym) psi-mi:"MI:0813"(proximity ligation assay) Kläsener et al. (2014) imex:IM-23964|pubmed:24963139 taxid:9606(human)|taxid:9606(Homo sapiens) taxid:9606(human)|taxid:9606(Homo sapiens) psi-mi:"MI:2364"(proximity) psi-mi:"MI:0469"(IntAct) intact:EBI-10683823|imex:IM-23964-13 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) ensembl:ENSG00000177455(gene)|ensembl:ENST00000538922(transcript)|go:"GO:0001923"(B-1 B cell differentiation)|go:"GO:0002322"(B cell proliferation involved in immune response)|go:"GO:0005886"(plasma membrane)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0009897"(external side of plasma membrane)|go:"GO:0016064"(immunoglobulin mediated immune response)|go:"GO:0032991"(protein-containing complex)|go:"GO:0043552"(positive regulation of phosphatidylinositol 3-kinase activity)|go:"GO:0045121"(membrane raft)|go:"GO:0050851"(antigen receptor-mediated signaling pathway)|go:"GO:0050853"(B cell receptor signaling pathway)|go:"GO:0050855"(regulation of B cell receptor signaling pathway)|go:"GO:0050864"(regulation of B cell activation)|go:"GO:0051281"(positive regulation of release of sequestered calcium ion into cytosol)|go:"GO:0070062"(extracellular exosome)|interpro:IPR003599(Immunoglobulin subtype)|interpro:IPR007110(Immunoglobulin-like)|interpro:IPR013783(Immunoglobulin-like fold)|interpro:IPR036179|interpro:IPR042341|mint:P15391|rcsb pdb:6AL5|rcsb pdb:7JIC|reactome:R-HSA-1257604|reactome:R-HSA-198933|reactome:R-HSA-2219530|reactome:R-HSA-6811558|reactome:R-HSA-977606|reactome:R-HSA-983695|refseq:NP_001171569.1|refseq:NP_001761.3 reactome:R-HSA-5690714|interpro:IPR007110(Immunoglobulin-like)|interpro:IPR013783(Immunoglobulin-like fold)|interpro:IPR003006(Immunoglobulin/major histocompatibility complex, conserved site)|interpro:IPR003597(Immunoglobulin C1-set)|interpro:IPR013151(Immunoglobulin)|rcsb pdb:1ZVO|reactome:R-HSA-983695|go:"GO:0003823"(antigen binding)|go:"GO:0005886"(plasma membrane)|go:"GO:0006910"(phagocytosis, recognition)|go:"GO:0006911"(phagocytosis, engulfment)|go:"GO:0006955"(immune response)|go:"GO:0006958"(complement activation, classical pathway)|go:"GO:0009897"(external side of plasma membrane)|go:"GO:0016021"(integral component of membrane)|go:"GO:0032732"(positive regulation of interleukin-1 production)|go:"GO:0034987"(immunoglobulin receptor binding)|go:"GO:0042571"(immunoglobulin complex, circulating)|go:"GO:0042742"(defense response to bacterium)|go:"GO:0045087"(innate immune response)|go:"GO:0050853"(B cell receptor signaling pathway)|go:"GO:0050871"(positive regulation of B cell activation)|go:"GO:0070062"(extracellular exosome)|go:"GO:0072562"(blood microparticle)|interpro:IPR036179 - - - figure legend:7D|comment:CD19 and 20 are found together with the resting IgD-BCR and dissociate from this receptor upon BCR activation|antagonist:Specific antigen- NIP|dataset:Affinomics - Interactions curated for the Affinomics consortium.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:9606(human-b-cell)|taxid:9606(Homo sapiens B cell) - 2015/02/13 2017/03/30 rogid:RXBn8jYrGc1LM3POARtQeEzKWoI9606 rogid:VsBOFKcu7mePDLkfpASjGVloqx89606 intact-crc:B71E298B63B4CACE|rigid:FSOHl5vK3Q2tI5qTOAmvq0rR9R8 false - - - - psi-mi:"MI:0421"(identification by antibody) psi-mi:"MI:0421"(identification by antibody) uniprotkb:P01880 uniprotkb:P11836 intact:EBI-10683716|uniprotkb:A0A087WUS7|uniprotkb:Q6P4I8|uniprotkb:Q8WU38 intact:EBI-2808234|ensembl:ENSP00000314620|ensembl:ENSP00000374589|ensembl:ENSP00000433277|ensembl:ENSP00000501369|uniprotkb:A6NMS4|uniprotkb:P08984|uniprotkb:Q13963|uniprotkb:B4DT24 psi-mi:ighd_human(display_long)|uniprotkb:IGHD(gene name)|psi-mi:IGHD(display_short)|uniprotkb:Ig delta chain C region(gene name synonym)|uniprotkb:Ig delta chain C region NIG-65(gene name synonym)|uniprotkb:Ig delta chain C region WAH(gene name synonym) psi-mi:cd20_human(display_long)|uniprotkb:MS4A1(gene name)|psi-mi:MS4A1(display_short)|uniprotkb:CD20(gene name synonym)|uniprotkb:B-lymphocyte surface antigen B1(gene name synonym)|uniprotkb:Bp35(gene name synonym)|uniprotkb:Leukocyte surface antigen Leu-16(gene name synonym)|uniprotkb:Membrane-spanning 4-domains subfamily A member 1(gene name synonym) psi-mi:"MI:0813"(proximity ligation assay) Kläsener et al. (2014) imex:IM-23964|pubmed:24963139 taxid:9606(human)|taxid:9606(Homo sapiens) taxid:9606(human)|taxid:9606(Homo sapiens) psi-mi:"MI:2364"(proximity) psi-mi:"MI:0469"(IntAct) intact:EBI-10683817|imex:IM-23964-53 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) reactome:R-HSA-5690714|interpro:IPR007110(Immunoglobulin-like)|interpro:IPR013783(Immunoglobulin-like fold)|interpro:IPR003006(Immunoglobulin/major histocompatibility complex, conserved site)|interpro:IPR003597(Immunoglobulin C1-set)|interpro:IPR013151(Immunoglobulin)|rcsb pdb:1ZVO|reactome:R-HSA-983695|go:"GO:0003823"(antigen binding)|go:"GO:0005886"(plasma membrane)|go:"GO:0006910"(phagocytosis, recognition)|go:"GO:0006911"(phagocytosis, engulfment)|go:"GO:0006955"(immune response)|go:"GO:0006958"(complement activation, classical pathway)|go:"GO:0009897"(external side of plasma membrane)|go:"GO:0016021"(integral component of membrane)|go:"GO:0032732"(positive regulation of interleukin-1 production)|go:"GO:0034987"(immunoglobulin receptor binding)|go:"GO:0042571"(immunoglobulin complex, circulating)|go:"GO:0042742"(defense response to bacterium)|go:"GO:0045087"(innate immune response)|go:"GO:0050853"(B cell receptor signaling pathway)|go:"GO:0050871"(positive regulation of B cell activation)|go:"GO:0070062"(extracellular exosome)|go:"GO:0072562"(blood microparticle)|interpro:IPR036179 ensembl:ENSG00000156738(gene)|ensembl:ENST00000345732(transcript)|ensembl:ENST00000389939(transcript)|ensembl:ENST00000534668(transcript)|ensembl:ENST00000674194(transcript)|go:"GO:0002115"(store-operated calcium entry)|go:"GO:0005154"(epidermal growth factor receptor binding)|go:"GO:0005615"(extracellular space)|go:"GO:0005654"(nucleoplasm)|go:"GO:0005886"(plasma membrane)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0006959"(humoral immune response)|go:"GO:0009617"(response to bacterium)|go:"GO:0009897"(external side of plasma membrane)|go:"GO:0009986"(cell surface)|go:"GO:0019865"(immunoglobulin binding)|go:"GO:0023026"(MHC class II protein complex binding)|go:"GO:0030183"(B cell differentiation)|go:"GO:0042100"(B cell proliferation)|go:"GO:0042113"(B cell activation)|go:"GO:0042802"(identical protein binding)|go:"GO:0044853"(plasma membrane raft)|go:"GO:0050853"(B cell receptor signaling pathway)|go:"GO:0051262"(protein tetramerization)|go:"GO:0070062"(extracellular exosome)|go:"GO:1902656"(calcium ion import into cytosol)|interpro:IPR007237(CD20/IgE Fc receptor beta subunit)|interpro:IPR030417|interpro:IPR030418|rcsb pdb:1S8B|rcsb pdb:2OSL|rcsb pdb:3BKY|rcsb pdb:3PP4|rcsb pdb:6VJA|rcsb pdb:6Y90|rcsb pdb:6Y92|rcsb pdb:6Y97|rcsb pdb:6Y9A|refseq:NP_068769.2|refseq:NP_690605.1 - - - figure legend:7D-2|comment:CD19 and 20 are found together with the resting IgD-BCR and dissociate from this receptor upon BCR activation|antagonist:Specific antigen- NIP|dataset:Affinomics - Interactions curated for the Affinomics consortium.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:9606(human-b-cell)|taxid:9606(Homo sapiens B cell) - 2015/02/13 2017/03/30 rogid:VsBOFKcu7mePDLkfpASjGVloqx89606 rogid:AnlKRZ3H77etWyyZIldbXkJDRkk9606 intact-crc:8502A1BBD55A1260|rigid:rB7h/wo6VMYjieJ+MpqJVUDMV7Q false - - - - psi-mi:"MI:0421"(identification by antibody) psi-mi:"MI:0421"(identification by antibody) uniprotkb:P01871 uniprotkb:P11836 intact:EBI-953797|intact:EBI-28974488|uniprotkb:P20769|uniprotkb:A0A075B6N9|uniprotkb:A0A0G2JQL4|uniprotkb:P04220|ensembl:ENSP00000375001|ensembl:ENSP00000485962 intact:EBI-2808234|ensembl:ENSP00000314620|ensembl:ENSP00000374589|ensembl:ENSP00000433277|ensembl:ENSP00000501369|uniprotkb:A6NMS4|uniprotkb:P08984|uniprotkb:Q13963|uniprotkb:B4DT24 psi-mi:ighm_human(display_long)|uniprotkb:Ig mu chain C region BOT(gene name synonym)|uniprotkb:IGHM(gene name)|psi-mi:IGHM(display_short)|uniprotkb:Ig mu chain C region(gene name synonym)|uniprotkb:Ig mu chain C region GAL(gene name synonym)|uniprotkb:Ig mu chain C region OU(gene name synonym) psi-mi:cd20_human(display_long)|uniprotkb:MS4A1(gene name)|psi-mi:MS4A1(display_short)|uniprotkb:CD20(gene name synonym)|uniprotkb:B-lymphocyte surface antigen B1(gene name synonym)|uniprotkb:Bp35(gene name synonym)|uniprotkb:Leukocyte surface antigen Leu-16(gene name synonym)|uniprotkb:Membrane-spanning 4-domains subfamily A member 1(gene name synonym) psi-mi:"MI:0813"(proximity ligation assay) Kläsener et al. (2014) imex:IM-23964|pubmed:24963139 taxid:9606(human)|taxid:9606(Homo sapiens) taxid:9606(human)|taxid:9606(Homo sapiens) psi-mi:"MI:2364"(proximity) psi-mi:"MI:0469"(IntAct) intact:EBI-10683771|imex:IM-23964-7 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) ensembl:ENSP00000375001|ensembl:ENSP00000485962|ensembl:ENST00000637539|ensembl:ENSP00000490253|go:"GO:0009897"(external side of plasma membrane)|go:"GO:0009986"(cell surface)|go:"GO:0016021"(integral component of membrane)|go:"GO:0019731"(antibacterial humoral response)|go:"GO:0042571"(immunoglobulin complex, circulating)|go:"GO:0042742"(defense response to bacterium)|go:"GO:0045087"(innate immune response)|go:"GO:0050829"(defense response to Gram-negative bacterium)|go:"GO:0050853"(B cell receptor signaling pathway)|go:"GO:0050871"(positive regulation of B cell activation)|go:"GO:0070062"(extracellular exosome)|go:"GO:0071756"(pentameric IgM immunoglobulin complex)|go:"GO:0071757"(hexameric IgM immunoglobulin complex)|go:"GO:0072562"(blood microparticle)|interpro:IPR003006(Immunoglobulin/major histocompatibility complex, conserved site)|interpro:IPR003597(Immunoglobulin C1-set)|interpro:IPR007110(Immunoglobulin-like)|interpro:IPR013783(Immunoglobulin-like fold)|interpro:IPR036179|mint:P01871|rcsb pdb:1HEZ|rcsb pdb:2AGJ|rcsb pdb:2RCJ|rcsb pdb:6KXS|rcsb pdb:7K0C|reactome:R-HSA-202733|reactome:R-HSA-5690714|reactome:R-HSA-983695|ensembl:ENSG00000211899(gene)|ensembl:ENSG00000282657(gene)|ensembl:ENST00000390559(transcript)|ensembl:ENST00000626472(transcript)|go:"GO:0002250"(adaptive immune response)|go:"GO:0003823"(antigen binding)|go:"GO:0005615"(extracellular space)|go:"GO:0005886"(plasma membrane)|go:"GO:0006910"(phagocytosis, recognition)|go:"GO:0006911"(phagocytosis, engulfment)|go:"GO:0006958"(complement activation, classical pathway) ensembl:ENSG00000156738(gene)|ensembl:ENST00000345732(transcript)|ensembl:ENST00000389939(transcript)|ensembl:ENST00000534668(transcript)|ensembl:ENST00000674194(transcript)|go:"GO:0002115"(store-operated calcium entry)|go:"GO:0005154"(epidermal growth factor receptor binding)|go:"GO:0005615"(extracellular space)|go:"GO:0005654"(nucleoplasm)|go:"GO:0005886"(plasma membrane)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0006959"(humoral immune response)|go:"GO:0009617"(response to bacterium)|go:"GO:0009897"(external side of plasma membrane)|go:"GO:0009986"(cell surface)|go:"GO:0019865"(immunoglobulin binding)|go:"GO:0023026"(MHC class II protein complex binding)|go:"GO:0030183"(B cell differentiation)|go:"GO:0042100"(B cell proliferation)|go:"GO:0042113"(B cell activation)|go:"GO:0042802"(identical protein binding)|go:"GO:0044853"(plasma membrane raft)|go:"GO:0050853"(B cell receptor signaling pathway)|go:"GO:0051262"(protein tetramerization)|go:"GO:0070062"(extracellular exosome)|go:"GO:1902656"(calcium ion import into cytosol)|interpro:IPR007237(CD20/IgE Fc receptor beta subunit)|interpro:IPR030417|interpro:IPR030418|rcsb pdb:1S8B|rcsb pdb:2OSL|rcsb pdb:3BKY|rcsb pdb:3PP4|rcsb pdb:6VJA|rcsb pdb:6Y90|rcsb pdb:6Y92|rcsb pdb:6Y97|rcsb pdb:6Y9A|refseq:NP_068769.2|refseq:NP_690605.1 - - - figure legend:7C-2|comment:CD20 is only found in close association with the activated but not with the resting IgM-BCR.|agonist:Specific antigen- NIP|dataset:Affinomics - Interactions curated for the Affinomics consortium.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:9606(human-b-cell)|taxid:9606(Homo sapiens B cell) - 2015/02/13 2017/03/30 rogid:UMG2n3hbO3ecgM7+WS7M/l5b/Eg9606 rogid:AnlKRZ3H77etWyyZIldbXkJDRkk9606 intact-crc:8CD3CF692CCB3A92|rigid:exotuCO+jAP/kFPhAvYGDKERQ/Q false - - - - psi-mi:"MI:0421"(identification by antibody) psi-mi:"MI:0421"(identification by antibody) uniprotkb:P01871 uniprotkb:P01871 intact:EBI-953797|intact:EBI-28974488|uniprotkb:P20769|uniprotkb:A0A075B6N9|uniprotkb:A0A0G2JQL4|uniprotkb:P04220|ensembl:ENSP00000375001|ensembl:ENSP00000485962 intact:EBI-953797|intact:EBI-28974488|uniprotkb:P20769|uniprotkb:A0A075B6N9|uniprotkb:A0A0G2JQL4|uniprotkb:P04220|ensembl:ENSP00000375001|ensembl:ENSP00000485962 psi-mi:ighm_human(display_long)|uniprotkb:Ig mu chain C region BOT(gene name synonym)|uniprotkb:IGHM(gene name)|psi-mi:IGHM(display_short)|uniprotkb:Ig mu chain C region(gene name synonym)|uniprotkb:Ig mu chain C region GAL(gene name synonym)|uniprotkb:Ig mu chain C region OU(gene name synonym) psi-mi:ighm_human(display_long)|uniprotkb:Ig mu chain C region BOT(gene name synonym)|uniprotkb:IGHM(gene name)|psi-mi:IGHM(display_short)|uniprotkb:Ig mu chain C region(gene name synonym)|uniprotkb:Ig mu chain C region GAL(gene name synonym)|uniprotkb:Ig mu chain C region OU(gene name synonym) psi-mi:"MI:0813"(proximity ligation assay) Kläsener et al. (2014) imex:IM-23964|pubmed:24963139 taxid:9606(human)|taxid:9606(Homo sapiens) taxid:9606(human)|taxid:9606(Homo sapiens) psi-mi:"MI:2364"(proximity) psi-mi:"MI:0469"(IntAct) intact:EBI-10683535|imex:IM-23964-45 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) ensembl:ENSP00000375001|ensembl:ENSP00000485962|ensembl:ENST00000637539|ensembl:ENSP00000490253|go:"GO:0009897"(external side of plasma membrane)|go:"GO:0009986"(cell surface)|go:"GO:0016021"(integral component of membrane)|go:"GO:0019731"(antibacterial humoral response)|go:"GO:0042571"(immunoglobulin complex, circulating)|go:"GO:0042742"(defense response to bacterium)|go:"GO:0045087"(innate immune response)|go:"GO:0050829"(defense response to Gram-negative bacterium)|go:"GO:0050853"(B cell receptor signaling pathway)|go:"GO:0050871"(positive regulation of B cell activation)|go:"GO:0070062"(extracellular exosome)|go:"GO:0071756"(pentameric IgM immunoglobulin complex)|go:"GO:0071757"(hexameric IgM immunoglobulin complex)|go:"GO:0072562"(blood microparticle)|interpro:IPR003006(Immunoglobulin/major histocompatibility complex, conserved site)|interpro:IPR003597(Immunoglobulin C1-set)|interpro:IPR007110(Immunoglobulin-like)|interpro:IPR013783(Immunoglobulin-like fold)|interpro:IPR036179|mint:P01871|rcsb pdb:1HEZ|rcsb pdb:2AGJ|rcsb pdb:2RCJ|rcsb pdb:6KXS|rcsb pdb:7K0C|reactome:R-HSA-202733|reactome:R-HSA-5690714|reactome:R-HSA-983695|ensembl:ENSG00000211899(gene)|ensembl:ENSG00000282657(gene)|ensembl:ENST00000390559(transcript)|ensembl:ENST00000626472(transcript)|go:"GO:0002250"(adaptive immune response)|go:"GO:0003823"(antigen binding)|go:"GO:0005615"(extracellular space)|go:"GO:0005886"(plasma membrane)|go:"GO:0006910"(phagocytosis, recognition)|go:"GO:0006911"(phagocytosis, engulfment)|go:"GO:0006958"(complement activation, classical pathway) ensembl:ENSP00000375001|ensembl:ENSP00000485962|ensembl:ENST00000637539|ensembl:ENSP00000490253|go:"GO:0009897"(external side of plasma membrane)|go:"GO:0009986"(cell surface)|go:"GO:0016021"(integral component of membrane)|go:"GO:0019731"(antibacterial humoral response)|go:"GO:0042571"(immunoglobulin complex, circulating)|go:"GO:0042742"(defense response to bacterium)|go:"GO:0045087"(innate immune response)|go:"GO:0050829"(defense response to Gram-negative bacterium)|go:"GO:0050853"(B cell receptor signaling pathway)|go:"GO:0050871"(positive regulation of B cell activation)|go:"GO:0070062"(extracellular exosome)|go:"GO:0071756"(pentameric IgM immunoglobulin complex)|go:"GO:0071757"(hexameric IgM immunoglobulin complex)|go:"GO:0072562"(blood microparticle)|interpro:IPR003006(Immunoglobulin/major histocompatibility complex, conserved site)|interpro:IPR003597(Immunoglobulin C1-set)|interpro:IPR007110(Immunoglobulin-like)|interpro:IPR013783(Immunoglobulin-like fold)|interpro:IPR036179|mint:P01871|rcsb pdb:1HEZ|rcsb pdb:2AGJ|rcsb pdb:2RCJ|rcsb pdb:6KXS|rcsb pdb:7K0C|reactome:R-HSA-202733|reactome:R-HSA-5690714|reactome:R-HSA-983695|ensembl:ENSG00000211899(gene)|ensembl:ENSG00000282657(gene)|ensembl:ENST00000390559(transcript)|ensembl:ENST00000626472(transcript)|go:"GO:0002250"(adaptive immune response)|go:"GO:0003823"(antigen binding)|go:"GO:0005615"(extracellular space)|go:"GO:0005886"(plasma membrane)|go:"GO:0006910"(phagocytosis, recognition)|go:"GO:0006911"(phagocytosis, engulfment)|go:"GO:0006958"(complement activation, classical pathway) - - - figure legend:4D|antagonist:Pervanadate, Lat A;BCR-activating drugs|dataset:Affinomics - Interactions curated for the Affinomics consortium.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:9606(human-b-cell)|taxid:9606(Homo sapiens B cell) - 2015/02/13 2017/03/30 rogid:UMG2n3hbO3ecgM7+WS7M/l5b/Eg9606 rogid:UMG2n3hbO3ecgM7+WS7M/l5b/Eg9606 intact-crc:193F08C4A219E295|rigid:YeHk3LKdcx984E6HU1pXkzsn3Cw false - - - - psi-mi:"MI:0421"(identification by antibody) psi-mi:"MI:0421"(identification by antibody) uniprotkb:P01880 uniprotkb:P01880 intact:EBI-10683716|uniprotkb:A0A087WUS7|uniprotkb:Q6P4I8|uniprotkb:Q8WU38 intact:EBI-10683716|uniprotkb:A0A087WUS7|uniprotkb:Q6P4I8|uniprotkb:Q8WU38 psi-mi:ighd_human(display_long)|uniprotkb:IGHD(gene name)|psi-mi:IGHD(display_short)|uniprotkb:Ig delta chain C region(gene name synonym)|uniprotkb:Ig delta chain C region NIG-65(gene name synonym)|uniprotkb:Ig delta chain C region WAH(gene name synonym) psi-mi:ighd_human(display_long)|uniprotkb:IGHD(gene name)|psi-mi:IGHD(display_short)|uniprotkb:Ig delta chain C region(gene name synonym)|uniprotkb:Ig delta chain C region NIG-65(gene name synonym)|uniprotkb:Ig delta chain C region WAH(gene name synonym) psi-mi:"MI:0813"(proximity ligation assay) Kläsener et al. (2014) imex:IM-23964|pubmed:24963139 taxid:9606(human)|taxid:9606(Homo sapiens) taxid:9606(human)|taxid:9606(Homo sapiens) psi-mi:"MI:2364"(proximity) psi-mi:"MI:0469"(IntAct) intact:EBI-10683711|imex:IM-23964-5 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) reactome:R-HSA-5690714|interpro:IPR007110(Immunoglobulin-like)|interpro:IPR013783(Immunoglobulin-like fold)|interpro:IPR003006(Immunoglobulin/major histocompatibility complex, conserved site)|interpro:IPR003597(Immunoglobulin C1-set)|interpro:IPR013151(Immunoglobulin)|rcsb pdb:1ZVO|reactome:R-HSA-983695|go:"GO:0003823"(antigen binding)|go:"GO:0005886"(plasma membrane)|go:"GO:0006910"(phagocytosis, recognition)|go:"GO:0006911"(phagocytosis, engulfment)|go:"GO:0006955"(immune response)|go:"GO:0006958"(complement activation, classical pathway)|go:"GO:0009897"(external side of plasma membrane)|go:"GO:0016021"(integral component of membrane)|go:"GO:0032732"(positive regulation of interleukin-1 production)|go:"GO:0034987"(immunoglobulin receptor binding)|go:"GO:0042571"(immunoglobulin complex, circulating)|go:"GO:0042742"(defense response to bacterium)|go:"GO:0045087"(innate immune response)|go:"GO:0050853"(B cell receptor signaling pathway)|go:"GO:0050871"(positive regulation of B cell activation)|go:"GO:0070062"(extracellular exosome)|go:"GO:0072562"(blood microparticle)|interpro:IPR036179 reactome:R-HSA-5690714|interpro:IPR007110(Immunoglobulin-like)|interpro:IPR013783(Immunoglobulin-like fold)|interpro:IPR003006(Immunoglobulin/major histocompatibility complex, conserved site)|interpro:IPR003597(Immunoglobulin C1-set)|interpro:IPR013151(Immunoglobulin)|rcsb pdb:1ZVO|reactome:R-HSA-983695|go:"GO:0003823"(antigen binding)|go:"GO:0005886"(plasma membrane)|go:"GO:0006910"(phagocytosis, recognition)|go:"GO:0006911"(phagocytosis, engulfment)|go:"GO:0006955"(immune response)|go:"GO:0006958"(complement activation, classical pathway)|go:"GO:0009897"(external side of plasma membrane)|go:"GO:0016021"(integral component of membrane)|go:"GO:0032732"(positive regulation of interleukin-1 production)|go:"GO:0034987"(immunoglobulin receptor binding)|go:"GO:0042571"(immunoglobulin complex, circulating)|go:"GO:0042742"(defense response to bacterium)|go:"GO:0045087"(innate immune response)|go:"GO:0050853"(B cell receptor signaling pathway)|go:"GO:0050871"(positive regulation of B cell activation)|go:"GO:0070062"(extracellular exosome)|go:"GO:0072562"(blood microparticle)|interpro:IPR036179 - - - figure legend:4D|comment:The Fab-PLA signal is lost upon exposure of B cells to antigen, or in presence of BCR-activating drugs.|antagonist:"specific antigen-NIP, pervanadate (Perv) and Lat-A - activators of the BCR."|dataset:Affinomics - Interactions curated for the Affinomics consortium.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:9606(human-b-cell)|taxid:9606(Homo sapiens B cell) - 2015/02/13 2017/03/30 rogid:VsBOFKcu7mePDLkfpASjGVloqx89606 rogid:VsBOFKcu7mePDLkfpASjGVloqx89606 intact-crc:0228A41B6E8867E3|rigid:GZ4FysJdB/KOxyqu+4Uncnmih04 false - - - - psi-mi:"MI:0421"(identification by antibody) psi-mi:"MI:0421"(identification by antibody) uniprotkb:P01882 uniprotkb:P01882 intact:EBI-10683684 intact:EBI-10683684 psi-mi:ighdm_mouse(display_long) psi-mi:ighdm_mouse(display_long) psi-mi:"MI:0813"(proximity ligation assay) Kläsener et al. (2014) imex:IM-23964|pubmed:24963139 taxid:10090(mouse)|taxid:10090(Mus musculus) taxid:10090(mouse)|taxid:10090(Mus musculus) psi-mi:"MI:2364"(proximity) psi-mi:"MI:0469"(IntAct) intact:EBI-10683682|imex:IM-23964-63 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) interpro:IPR007110(Immunoglobulin-like)|interpro:IPR013783(Immunoglobulin-like fold)|interpro:IPR003006(Immunoglobulin/major histocompatibility complex, conserved site)|interpro:IPR003597(Immunoglobulin C1-set)|go:"GO:0005886"(plasma membrane)|go:"GO:0016021"(integral component of membrane)|interpro:IPR036179 interpro:IPR007110(Immunoglobulin-like)|interpro:IPR013783(Immunoglobulin-like fold)|interpro:IPR003006(Immunoglobulin/major histocompatibility complex, conserved site)|interpro:IPR003597(Immunoglobulin C1-set)|go:"GO:0005886"(plasma membrane)|go:"GO:0016021"(integral component of membrane)|interpro:IPR036179 - - - figure legend:3B|comment:"No oligomerisation of IgD was seen when an IgD mutant was transfected. (exact mutations are not stated.)"|dataset:Affinomics - Interactions curated for the Affinomics consortium.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:7227(drome-schneider_2)|taxid:7227(Drosophila melanogaster late embryo cells) - 2015/02/13 2015/02/24 rogid:WPb0XrDhxuYDQ0jFtnq2QDd6u1c10090 rogid:WPb0XrDhxuYDQ0jFtnq2QDd6u1c10090 intact-crc:578EE51DB8A1D304|rigid:Kbyvm7r3c+tkujEdaJCVrV1B61A false - - - 2 psi-mi:"MI:0421"(identification by antibody) psi-mi:"MI:0421"(identification by antibody) uniprotkb:P01872 uniprotkb:P01872 intact:EBI-645833|uniprotkb:P01873 intact:EBI-645833|uniprotkb:P01873 psi-mi:ighm_mouse(display_long)|uniprotkb:Ighm(gene name)|psi-mi:Ighm(display_short)|uniprotkb:Igh-6(gene name synonym) psi-mi:ighm_mouse(display_long)|uniprotkb:Ighm(gene name)|psi-mi:Ighm(display_short)|uniprotkb:Igh-6(gene name synonym) psi-mi:"MI:0071"(molecular sieving) Kläsener et al. (2014) imex:IM-23964|pubmed:24963139 taxid:10090(mouse)|taxid:10090(Mus musculus) taxid:10090(mouse)|taxid:10090(Mus musculus) psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-10758548|imex:IM-23964-72 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) interpro:IPR007110(Immunoglobulin-like)|interpro:IPR013783(Immunoglobulin-like fold)|interpro:IPR003006(Immunoglobulin/major histocompatibility complex, conserved site)|interpro:IPR003597(Immunoglobulin C1-set)|go:"GO:0002344"(B cell affinity maturation)|go:"GO:0002455"(humoral immune response mediated by circulating immunoglobulin)|go:"GO:0002637"(regulation of immunoglobulin production)|go:"GO:0003823"(antigen binding)|go:"GO:0004888"(transmembrane signaling receptor activity)|go:"GO:0005615"(extracellular space)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0006910"(phagocytosis, recognition)|go:"GO:0006911"(phagocytosis, engulfment)|go:"GO:0006958"(complement activation, classical pathway)|go:"GO:0009897"(external side of plasma membrane)|go:"GO:0009986"(cell surface)|go:"GO:0016020"(membrane)|go:"GO:0016021"(integral component of membrane)|go:"GO:0016064"(immunoglobulin mediated immune response)|go:"GO:0019815"(B cell receptor complex)|go:"GO:0019882"(antigen processing and presentation)|go:"GO:0022604"(regulation of cell morphogenesis)|go:"GO:0030890"(positive regulation of B cell proliferation)|go:"GO:0034987"(immunoglobulin receptor binding)|go:"GO:0042571"(immunoglobulin complex, circulating)|go:"GO:0042742"(defense response to bacterium)|go:"GO:0042802"(identical protein binding)|go:"GO:0043410"(positive regulation of MAPK cascade)|go:"GO:0045022"(early endosome to late endosome transport)|go:"GO:0045087"(innate immune response)|go:"GO:0045807"(positive regulation of endocytosis)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0050731"(positive regulation of peptidyl-tyrosine phosphorylation)|go:"GO:0050778"(positive regulation of immune response)|go:"GO:0050829"(defense response to Gram-negative bacterium)|go:"GO:0050853"(B cell receptor signaling pathway)|go:"GO:0050871"(positive regulation of B cell activation)|rcsb pdb:4BA8|rcsb pdb:4JVU|rcsb pdb:4JVW|reactome:R-MMU-202733|reactome:R-MMU-5690714|reactome:R-MMU-983695|interpro:IPR036179|mint:P01872 interpro:IPR007110(Immunoglobulin-like)|interpro:IPR013783(Immunoglobulin-like fold)|interpro:IPR003006(Immunoglobulin/major histocompatibility complex, conserved site)|interpro:IPR003597(Immunoglobulin C1-set)|go:"GO:0002344"(B cell affinity maturation)|go:"GO:0002455"(humoral immune response mediated by circulating immunoglobulin)|go:"GO:0002637"(regulation of immunoglobulin production)|go:"GO:0003823"(antigen binding)|go:"GO:0004888"(transmembrane signaling receptor activity)|go:"GO:0005615"(extracellular space)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0006910"(phagocytosis, recognition)|go:"GO:0006911"(phagocytosis, engulfment)|go:"GO:0006958"(complement activation, classical pathway)|go:"GO:0009897"(external side of plasma membrane)|go:"GO:0009986"(cell surface)|go:"GO:0016020"(membrane)|go:"GO:0016021"(integral component of membrane)|go:"GO:0016064"(immunoglobulin mediated immune response)|go:"GO:0019815"(B cell receptor complex)|go:"GO:0019882"(antigen processing and presentation)|go:"GO:0022604"(regulation of cell morphogenesis)|go:"GO:0030890"(positive regulation of B cell proliferation)|go:"GO:0034987"(immunoglobulin receptor binding)|go:"GO:0042571"(immunoglobulin complex, circulating)|go:"GO:0042742"(defense response to bacterium)|go:"GO:0042802"(identical protein binding)|go:"GO:0043410"(positive regulation of MAPK cascade)|go:"GO:0045022"(early endosome to late endosome transport)|go:"GO:0045087"(innate immune response)|go:"GO:0045807"(positive regulation of endocytosis)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0050731"(positive regulation of peptidyl-tyrosine phosphorylation)|go:"GO:0050778"(positive regulation of immune response)|go:"GO:0050829"(defense response to Gram-negative bacterium)|go:"GO:0050853"(B cell receptor signaling pathway)|go:"GO:0050871"(positive regulation of B cell activation)|rcsb pdb:4BA8|rcsb pdb:4JVU|rcsb pdb:4JVW|reactome:R-MMU-202733|reactome:R-MMU-5690714|reactome:R-MMU-983695|interpro:IPR036179|mint:P01872 - - - figure legend:2B|dataset:Affinomics - Interactions curated for the Affinomics consortium.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/02/13 2015/03/27 rogid:4+PnUlhyUfPZS43gGZMtyePhlCw10090 rogid:4+PnUlhyUfPZS43gGZMtyePhlCw10090 rigid:Ds7woaMkohaLVQdhA+Oztdzk4Vk false - - 5 0 psi-mi:"MI:0396"(predetermined participant) psi-mi:"MI:0396"(predetermined participant)