#ID(s) interactor A	ID(s) interactor B	Alt. ID(s) interactor A	Alt. ID(s) interactor B	Alias(es) interactor A	Alias(es) interactor B	Interaction detection method(s)	Publication 1st author(s)	Publication Identifier(s)	Taxid interactor A	Taxid interactor B	Interaction type(s)	Source database(s)	Interaction identifier(s)	Confidence value(s)	Expansion method(s)	Biological role(s) interactor A	Biological role(s) interactor B	Experimental role(s) interactor A	Experimental role(s) interactor B	Type(s) interactor A	Type(s) interactor B	Xref(s) interactor A	Xref(s) interactor B	Interaction Xref(s)	Annotation(s) interactor A	Annotation(s) interactor B	Interaction annotation(s)	Host organism(s)	Interaction parameter(s)	Creation date	Update date	Checksum(s) interactor A	Checksum(s) interactor B	Interaction Checksum(s)	Negative	Feature(s) interactor A	Feature(s) interactor B	Stoichiometry(s) interactor A	Stoichiometry(s) interactor B	Identification method participant A	Identification method participant B
uniprotkb:Q04226	uniprotkb:P13393	intact:EBI-18884|uniprotkb:D6VZF9|ensemblfungi:YML015C	intact:EBI-19129|uniprotkb:D3DM55|ensemblfungi:YER148W	psi-mi:taf11_yeast(display_long)|uniprotkb:TAF11(gene name)|psi-mi:TAF11(display_short)|uniprotkb:TAF40(gene name synonym)|uniprotkb:YML015C(locus name)|uniprotkb:YM9571.03C(orf name)|uniprotkb:TBP-associated factor 11(gene name synonym)|uniprotkb:TBP-associated factor 40 kDa(gene name synonym)|uniprotkb:TAFII-40(gene name synonym)	psi-mi:tbp_yeast(display_long)|uniprotkb:YER148W(locus name)|uniprotkb:SPT15(gene name)|psi-mi:SPT15(display_short)|uniprotkb:BTF1(gene name synonym)|uniprotkb:TBP1(gene name synonym)|uniprotkb:TATA-box factor(gene name synonym)|uniprotkb:TATA-binding factor(gene name synonym)|uniprotkb:TATA sequence-binding protein(gene name synonym)|uniprotkb:Transcription factor D(gene name synonym)|uniprotkb:Transcription initiation factor TFIID TBP subunit(gene name synonym)	psi-mi:"MI:0007"(anti tag coimmunoprecipitation)	Saint et al. (2014)	pubmed:24550006|imex:IM-24168	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-10821508|imex:IM-24168-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_013697.1|sgd:S000004477|go:"GO:0006366"(transcription by RNA polymerase II)|go:"GO:0046982"(protein heterodimerization activity)|go:"GO:0051123"(RNA polymerase II preinitiation complex assembly)|interpro:IPR006809(TAFII28-like protein)|interpro:IPR009072(Histone-fold)|reactome:R-SCE-674695|ensemblfungi:YML015C(gene)|ensemblfungi:YML015C_mRNA(transcript)|go:"GO:0003682"(chromatin binding)|go:"GO:0003713"(transcription coactivator activity)|go:"GO:0005669"(transcription factor TFIID complex)|reactome:R-SCE-73776|reactome:R-SCE-73779|reactome:R-SCE-75953|reactome:R-SCE-76042|reactome:R-SCE-6807505|dip:DIP-1686N|mint:Q04226	sgd:S000000950|ensemblfungi:YER148W(gene)|ensemblfungi:YER148W_mRNA(transcript)|go:"GO:0000126"(transcription factor TFIIIB complex)|go:"GO:0000979"(RNA polymerase II core promoter sequence-specific DNA binding)|go:"GO:0000995"(RNA polymerase III general transcription initiation factor activity)|go:"GO:0001016"(RNA polymerase III transcription regulatory region sequence-specific DNA binding)|go:"GO:0001092"(TFIIA-class transcription factor complex binding)|go:"GO:0001179"(RNA polymerase I general transcription initiation factor binding)|go:"GO:0001188"(RNA polymerase I preinitiation complex assembly)|go:"GO:0003682"(chromatin binding)|go:"GO:0005634"(nucleus)|go:"GO:0005654"(nucleoplasm)|go:"GO:0005669"(transcription factor TFIID complex)|go:"GO:0005672"(transcription factor TFIIA complex)|go:"GO:0006352"(DNA-templated transcription, initiation)|go:"GO:0008301"(DNA binding, bending)|go:"GO:0016251"(RNA polymerase II general transcription initiation factor activity)|go:"GO:0032991"(protein-containing complex)|go:"GO:0042790"(nucleolar large rRNA transcription by RNA polymerase I)|go:"GO:0051123"(RNA polymerase II preinitiation complex assembly)|go:"GO:0061629"(RNA polymerase II-specific DNA-binding transcription factor binding)|go:"GO:0070893"(transposon integration)|go:"GO:0070898"(RNA polymerase III preinitiation complex assembly)|go:"GO:0097718"(disordered domain specific binding)|go:"GO:0140223"(general transcription initiation factor activity)|go:"GO:0140297"(DNA-binding transcription factor binding)|interpro:IPR000814(TATA-box binding)|interpro:IPR012295(Beta2-adaptin/TATA-box binding, C-terminal)|interpro:IPR030491|interpro:IPR033710|rcsb pdb:1NGM|rcsb pdb:1NH2|rcsb pdb:1RM1|rcsb pdb:1TBA|rcsb pdb:1TBP|rcsb pdb:1YTB|rcsb pdb:1YTF|rcsb pdb:4B0A|rcsb pdb:4V1N|rcsb pdb:4V1O|rcsb pdb:5FMF|rcsb pdb:5FYW|rcsb pdb:5FZ5|rcsb pdb:5OQJ|rcsb pdb:5OQM|rcsb pdb:5SVA|rcsb pdb:6CNB|rcsb pdb:6CNC|rcsb pdb:6CND|rcsb pdb:6CNF|rcsb pdb:6E16|rcsb pdb:6E24|rcsb pdb:6EU0|rcsb pdb:6F40|rcsb pdb:6F41|rcsb pdb:6F42|rcsb pdb:6F44|rcsb pdb:6GYK|rcsb pdb:6GYL|rcsb pdb:6GYM|rcsb pdb:6TBM|rcsb pdb:7O4I|rcsb pdb:7O4J|rcsb pdb:7O72|rcsb pdb:7O73|rcsb pdb:7O75|reactome:R-SCE-674695|reactome:R-SCE-6807505|reactome:R-SCE-73772|reactome:R-SCE-73776|reactome:R-SCE-73779|reactome:R-SCE-75953|reactome:R-SCE-76042|reactome:R-SCE-76061|reactome:R-SCE-76066|reactome:R-SCE-9018519|refseq:NP_011075.3|dip:DIP-44N|mint:P13393	-	-	crc64:50365FFF1D3718CE	figure legend:f5c|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	-	2015/05/19	2015/05/19	rogid:xgyBQglZA6b6xk5ElCLwnKK4CpU559292	rogid:fVZPztzKW8tuxDYYpZ7ZTFlq3Do559292	rigid:yfo1JVGlscKN4ixrAPBKeO06DW4	false	myc tag:?-?	-	-	-	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)
uniprotkb:Q04226	uniprotkb:Q03761	intact:EBI-18884|uniprotkb:D6VZF9|ensemblfungi:YML015C	intact:EBI-35097|uniprotkb:D6VSC8|ensemblfungi:YDR145W	psi-mi:taf11_yeast(display_long)|uniprotkb:TAF11(gene name)|psi-mi:TAF11(display_short)|uniprotkb:TAF40(gene name synonym)|uniprotkb:YML015C(locus name)|uniprotkb:YM9571.03C(orf name)|uniprotkb:TBP-associated factor 11(gene name synonym)|uniprotkb:TBP-associated factor 40 kDa(gene name synonym)|uniprotkb:TAFII-40(gene name synonym)	psi-mi:taf12_yeast(display_long)|uniprotkb:TAF12(gene name)|psi-mi:TAF12(display_short)|uniprotkb:TAF61(gene name synonym)|uniprotkb:TAF68(gene name synonym)|uniprotkb:YDR145W(locus name)|uniprotkb:YD8358.02(orf name)|uniprotkb:TBP-associated factor 12(gene name synonym)|uniprotkb:TBP-associated factor 61 kDa(gene name synonym)|uniprotkb:TAFII-61(gene name synonym)|uniprotkb:TAFII-68(gene name synonym)	psi-mi:"MI:0007"(anti tag coimmunoprecipitation)	Saint et al. (2014)	pubmed:24550006|imex:IM-24168	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-10821508|imex:IM-24168-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_013697.1|sgd:S000004477|go:"GO:0006366"(transcription by RNA polymerase II)|go:"GO:0046982"(protein heterodimerization activity)|go:"GO:0051123"(RNA polymerase II preinitiation complex assembly)|interpro:IPR006809(TAFII28-like protein)|interpro:IPR009072(Histone-fold)|reactome:R-SCE-674695|ensemblfungi:YML015C(gene)|ensemblfungi:YML015C_mRNA(transcript)|go:"GO:0003682"(chromatin binding)|go:"GO:0003713"(transcription coactivator activity)|go:"GO:0005669"(transcription factor TFIID complex)|reactome:R-SCE-73776|reactome:R-SCE-73779|reactome:R-SCE-75953|reactome:R-SCE-76042|reactome:R-SCE-6807505|dip:DIP-1686N|mint:Q04226	refseq:NP_010429.1|sgd:S000002552|mint:Q03761|dip:DIP-1302N|ensemblfungi:YDR145W(gene)|ensemblfungi:YDR145W_mRNA(transcript)|go:"GO:0000124"(SAGA complex)|go:"GO:0003677"(DNA binding)|go:"GO:0003682"(chromatin binding)|go:"GO:0005669"(transcription factor TFIID complex)|go:"GO:0006325"(chromatin organization)|go:"GO:0006366"(transcription by RNA polymerase II)|go:"GO:0016573"(histone acetylation)|go:"GO:0017025"(TBP-class protein binding)|go:"GO:0042802"(identical protein binding)|go:"GO:0046695"("SLIK (SAGA-like) complex")|go:"GO:0046982"(protein heterodimerization activity)|go:"GO:0051123"(RNA polymerase II preinitiation complex assembly)|go:"GO:0060090"(molecular adaptor activity)|go:"GO:0061629"(RNA polymerase II-specific DNA-binding transcription factor binding)|interpro:IPR003228(Transcription initiation factor TFIID)|interpro:IPR009072(Histone-fold)|interpro:IPR037794|rcsb pdb:6T9I|rcsb pdb:6T9J|rcsb pdb:6T9K|reactome:R-SCE-674695|reactome:R-SCE-73776|reactome:R-SCE-73779|reactome:R-SCE-75953|reactome:R-SCE-76042	-	-	crc64:A8FC84E46F8370D3	figure legend:f5c|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	-	2015/05/19	2015/05/19	rogid:xgyBQglZA6b6xk5ElCLwnKK4CpU559292	rogid:DCTuNehYD4fhp7PRgtHZyNIDbH4559292	rigid:yfo1JVGlscKN4ixrAPBKeO06DW4	false	myc tag:?-?	-	-	-	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)
uniprotkb:Q04226	uniprotkb:P38129	intact:EBI-18884|uniprotkb:D6VZF9|ensemblfungi:YML015C	intact:EBI-18868|ensemblfungi:YBR198C|uniprotkb:D6VQJ4	psi-mi:taf11_yeast(display_long)|uniprotkb:TAF11(gene name)|psi-mi:TAF11(display_short)|uniprotkb:TAF40(gene name synonym)|uniprotkb:YML015C(locus name)|uniprotkb:YM9571.03C(orf name)|uniprotkb:TBP-associated factor 11(gene name synonym)|uniprotkb:TBP-associated factor 40 kDa(gene name synonym)|uniprotkb:TAFII-40(gene name synonym)	psi-mi:taf5_yeast(display_long)|uniprotkb:TAF5(gene name)|psi-mi:TAF5(display_short)|uniprotkb:TAF90(gene name synonym)|uniprotkb:YBR198C(locus name)|uniprotkb:YBR1410(orf name)|uniprotkb:TBP-associated factor 5(gene name synonym)|uniprotkb:TBP-associated factor 90 kDa(gene name synonym)|uniprotkb:TAFII-90(gene name synonym)	psi-mi:"MI:0007"(anti tag coimmunoprecipitation)	Saint et al. (2014)	pubmed:24550006|imex:IM-24168	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-10821508|imex:IM-24168-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_013697.1|sgd:S000004477|go:"GO:0006366"(transcription by RNA polymerase II)|go:"GO:0046982"(protein heterodimerization activity)|go:"GO:0051123"(RNA polymerase II preinitiation complex assembly)|interpro:IPR006809(TAFII28-like protein)|interpro:IPR009072(Histone-fold)|reactome:R-SCE-674695|ensemblfungi:YML015C(gene)|ensemblfungi:YML015C_mRNA(transcript)|go:"GO:0003682"(chromatin binding)|go:"GO:0003713"(transcription coactivator activity)|go:"GO:0005669"(transcription factor TFIID complex)|reactome:R-SCE-73776|reactome:R-SCE-73779|reactome:R-SCE-75953|reactome:R-SCE-76042|reactome:R-SCE-6807505|dip:DIP-1686N|mint:Q04226	ensemblfungi:YBR198C(gene)|ensemblfungi:YBR198C_mRNA(transcript)|go:"GO:0000124"(SAGA complex)|go:"GO:0003682"(chromatin binding)|go:"GO:0005669"(transcription factor TFIID complex)|go:"GO:0006325"(chromatin organization)|go:"GO:0006355"(regulation of transcription, DNA-templated)|go:"GO:0006366"(transcription by RNA polymerase II)|go:"GO:0006367"(transcription initiation from RNA polymerase II promoter)|go:"GO:0016573"(histone acetylation)|go:"GO:0042802"(identical protein binding)|go:"GO:0043130"(ubiquitin binding)|go:"GO:0043966"(histone H3 acetylation)|go:"GO:0046695"("SLIK (SAGA-like) complex")|refseq:NP_009757.1|sgd:S000000402|go:"GO:0060090"(molecular adaptor activity)|interpro:IPR001680(WD40 repeat)|interpro:IPR006594(LisH dimerisation motif)|interpro:IPR007582(WD40 associated region in TFIID subunit)|interpro:IPR015943(WD40/YVTN repeat-like)|interpro:IPR019775|interpro:IPR020472|interpro:IPR036322|interpro:IPR037264|interpro:IPR037783|rcsb pdb:2J49|rcsb pdb:6T9I|rcsb pdb:6T9K|reactome:R-SCE-674695|reactome:R-SCE-6807505|reactome:R-SCE-73776|reactome:R-SCE-73779|reactome:R-SCE-75953|reactome:R-SCE-76042|go:"GO:0051123"(RNA polymerase II preinitiation complex assembly)|dip:DIP-740N|mint:P38129	-	-	crc64:B42315B8C752D0B6	figure legend:f5c|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	-	2015/05/19	2015/05/19	rogid:xgyBQglZA6b6xk5ElCLwnKK4CpU559292	rogid:QA3hzlcvZh26ejkE7QNXNFP/l2Y559292	rigid:yfo1JVGlscKN4ixrAPBKeO06DW4	false	myc tag:?-?	-	-	-	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)
uniprotkb:Q04226	uniprotkb:P53040	intact:EBI-18884|uniprotkb:D6VZF9|ensemblfungi:YML015C	intact:EBI-18876|uniprotkb:D6VU35|ensemblfungi:YGL112C	psi-mi:taf11_yeast(display_long)|uniprotkb:TAF11(gene name)|psi-mi:TAF11(display_short)|uniprotkb:TAF40(gene name synonym)|uniprotkb:YML015C(locus name)|uniprotkb:YM9571.03C(orf name)|uniprotkb:TBP-associated factor 11(gene name synonym)|uniprotkb:TBP-associated factor 40 kDa(gene name synonym)|uniprotkb:TAFII-40(gene name synonym)	psi-mi:taf6_yeast(display_long)|uniprotkb:TAF6(gene name)|psi-mi:TAF6(display_short)|uniprotkb:TAF60(gene name synonym)|uniprotkb:YGL112C(locus name)|uniprotkb:G2985(orf name)|uniprotkb:TBP-associated factor 6(gene name synonym)|uniprotkb:TBP-associated factor 60 kDa(gene name synonym)	psi-mi:"MI:0007"(anti tag coimmunoprecipitation)	Saint et al. (2014)	pubmed:24550006|imex:IM-24168	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-10821508|imex:IM-24168-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_013697.1|sgd:S000004477|go:"GO:0006366"(transcription by RNA polymerase II)|go:"GO:0046982"(protein heterodimerization activity)|go:"GO:0051123"(RNA polymerase II preinitiation complex assembly)|interpro:IPR006809(TAFII28-like protein)|interpro:IPR009072(Histone-fold)|reactome:R-SCE-674695|ensemblfungi:YML015C(gene)|ensemblfungi:YML015C_mRNA(transcript)|go:"GO:0003682"(chromatin binding)|go:"GO:0003713"(transcription coactivator activity)|go:"GO:0005669"(transcription factor TFIID complex)|reactome:R-SCE-73776|reactome:R-SCE-73779|reactome:R-SCE-75953|reactome:R-SCE-76042|reactome:R-SCE-6807505|dip:DIP-1686N|mint:Q04226	refseq:NP_011403.1|sgd:S000003080|interpro:IPR004823("TATA box binding protein associated factor (TAF)")|interpro:IPR009072(Histone-fold)|interpro:IPR011442(Protein of unknown function DUF1546)|go:"GO:2000144"(positive regulation of DNA-templated transcription, initiation)|interpro:IPR016024(Armadillo-type fold)|interpro:IPR037796|rcsb pdb:6T9I|rcsb pdb:6T9K|reactome:R-SCE-674695|reactome:R-SCE-6807505|reactome:R-SCE-73776|reactome:R-SCE-73779|reactome:R-SCE-75953|reactome:R-SCE-76042|ensemblfungi:YGL112C(gene)|ensemblfungi:YGL112C_mRNA(transcript)|go:"GO:0000124"(SAGA complex)|go:"GO:0003682"(chromatin binding)|go:"GO:0003713"(transcription coactivator activity)|go:"GO:0005634"(nucleus)|go:"GO:0005669"(transcription factor TFIID complex)|go:"GO:0005829"(cytosol)|go:"GO:0006325"(chromatin organization)|go:"GO:0006366"(transcription by RNA polymerase II)|go:"GO:0006367"(transcription initiation from RNA polymerase II promoter)|go:"GO:0016251"(RNA polymerase II general transcription initiation factor activity)|go:"GO:0016573"(histone acetylation)|go:"GO:0042802"(identical protein binding)|go:"GO:0045944"(positive regulation of transcription by RNA polymerase II)|go:"GO:0046695"("SLIK (SAGA-like) complex")|go:"GO:0046982"(protein heterodimerization activity)|go:"GO:0051123"(RNA polymerase II preinitiation complex assembly)|go:"GO:0060090"(molecular adaptor activity)|go:"GO:0061629"(RNA polymerase II-specific DNA-binding transcription factor binding)|dip:DIP-981N|mint:P53040	-	-	crc64:14B590E02C16F289	figure legend:f5c|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	-	2015/05/19	2015/05/19	rogid:xgyBQglZA6b6xk5ElCLwnKK4CpU559292	rogid:qmzBE/xAfQskdJqoI8oIqAIa3+A559292	rigid:yfo1JVGlscKN4ixrAPBKeO06DW4	false	myc tag:?-?	-	-	-	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)
uniprotkb:Q04226	uniprotkb:Q05027	intact:EBI-18884|uniprotkb:D6VZF9|ensemblfungi:YML015C	intact:EBI-27500|uniprotkb:D6W062|ensemblfungi:YMR236W	psi-mi:taf11_yeast(display_long)|uniprotkb:TAF11(gene name)|psi-mi:TAF11(display_short)|uniprotkb:TAF40(gene name synonym)|uniprotkb:YML015C(locus name)|uniprotkb:YM9571.03C(orf name)|uniprotkb:TBP-associated factor 11(gene name synonym)|uniprotkb:TBP-associated factor 40 kDa(gene name synonym)|uniprotkb:TAFII-40(gene name synonym)	psi-mi:taf9_yeast(display_long)|uniprotkb:TAF9(gene name)|psi-mi:TAF9(display_short)|uniprotkb:TAF17(gene name synonym)|uniprotkb:YMR236W(locus name)|uniprotkb:YM9959.18(orf name)|uniprotkb:TBP-associated factor 9(gene name synonym)|uniprotkb:TBP-associated factor 17 kDa(gene name synonym)|uniprotkb:TAFII-17(gene name synonym)|uniprotkb:TAFII20(gene name synonym)	psi-mi:"MI:0007"(anti tag coimmunoprecipitation)	Saint et al. (2014)	pubmed:24550006|imex:IM-24168	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-10821508|imex:IM-24168-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_013697.1|sgd:S000004477|go:"GO:0006366"(transcription by RNA polymerase II)|go:"GO:0046982"(protein heterodimerization activity)|go:"GO:0051123"(RNA polymerase II preinitiation complex assembly)|interpro:IPR006809(TAFII28-like protein)|interpro:IPR009072(Histone-fold)|reactome:R-SCE-674695|ensemblfungi:YML015C(gene)|ensemblfungi:YML015C_mRNA(transcript)|go:"GO:0003682"(chromatin binding)|go:"GO:0003713"(transcription coactivator activity)|go:"GO:0005669"(transcription factor TFIID complex)|reactome:R-SCE-73776|reactome:R-SCE-73779|reactome:R-SCE-75953|reactome:R-SCE-76042|reactome:R-SCE-6807505|dip:DIP-1686N|mint:Q04226	mint:Q05027|refseq:NP_013963.1|sgd:S000004849|dip:DIP-925N|ensemblfungi:YMR236W(gene)|ensemblfungi:YMR236W_mRNA(transcript)|go:"GO:0000124"(SAGA complex)|go:"GO:0003682"(chromatin binding)|go:"GO:0003713"(transcription coactivator activity)|go:"GO:0005669"(transcription factor TFIID complex)|go:"GO:0006325"(chromatin organization)|go:"GO:0006366"(transcription by RNA polymerase II)|go:"GO:0016573"(histone acetylation)|go:"GO:0042802"(identical protein binding)|go:"GO:0043966"(histone H3 acetylation)|go:"GO:0045944"(positive regulation of transcription by RNA polymerase II)|go:"GO:0046695"("SLIK (SAGA-like) complex")|go:"GO:0046982"(protein heterodimerization activity)|go:"GO:0051123"(RNA polymerase II preinitiation complex assembly)|go:"GO:0060090"(molecular adaptor activity)|interpro:IPR003162(Transcription factor TAFII-31)|interpro:IPR009072(Histone-fold)|rcsb pdb:6T9I|rcsb pdb:6T9K|reactome:R-SCE-5689880|reactome:R-SCE-674695|reactome:R-SCE-6807505|reactome:R-SCE-73776|reactome:R-SCE-73779|reactome:R-SCE-75953|reactome:R-SCE-76042	-	-	crc64:D1C97EDDCA468EC5	figure legend:f5c|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	-	2015/05/19	2015/05/19	rogid:xgyBQglZA6b6xk5ElCLwnKK4CpU559292	rogid:3+S7ozz3r1gCMvBUUfkGpeTCy/k559292	rigid:yfo1JVGlscKN4ixrAPBKeO06DW4	false	myc tag:?-?	binding-associated region:1-131|ha tag:?-?	-	-	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)
uniprotkb:P35177	uniprotkb:Q03330	intact:EBI-17958|uniprotkb:D6VQ80|ensemblfungi:YBR081C	intact:EBI-7458|uniprotkb:Q6B165|uniprotkb:D6VV32|ensemblfungi:YGR252W	psi-mi:spt7_yeast(display_long)|uniprotkb:YBR0739(orf name)|uniprotkb:YBR081C(locus name)|uniprotkb:SPT7(gene name)|psi-mi:SPT7(display_short)	psi-mi:gcn5_yeast(display_long)|uniprotkb:YGR252W(locus name)|uniprotkb:GCN5(gene name)|psi-mi:GCN5(display_short)|uniprotkb:ADA4(gene name synonym)|uniprotkb:SWI9(gene name synonym)	psi-mi:"MI:0007"(anti tag coimmunoprecipitation)	Saint et al. (2014)	pubmed:24550006|imex:IM-24168	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-10821522|imex:IM-24168-3	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_009637.1|sgd:S000000285|ensemblfungi:YBR081C(gene)|ensemblfungi:YBR081C_mRNA(transcript)|go:"GO:0000124"(SAGA complex)|go:"GO:0000747"(conjugation with cellular fusion)|go:"GO:0005198"(structural molecule activity)|go:"GO:0005739"(mitochondrion)|go:"GO:0006325"(chromatin organization)|go:"GO:0006357"(regulation of transcription by RNA polymerase II)|go:"GO:0016573"(histone acetylation)|go:"GO:0034622"|go:"GO:0046695"("SLIK (SAGA-like) complex")|go:"GO:0046982"(protein heterodimerization activity)|interpro:IPR001487(Bromodomain)|interpro:IPR009072(Histone-fold)|interpro:IPR018359|interpro:IPR036427|interpro:IPR037782|rcsb pdb:6T9I|rcsb pdb:6T9K|dip:DIP-771N|mint:P35177	refseq:NP_011768.1|sgd:S000003484|mint:Q03330|dip:DIP-710N|ensemblfungi:YGR252W(gene)|ensemblfungi:YGR252W_mRNA(transcript)|go:"GO:0000124"(SAGA complex)|go:"GO:0000775"(chromosome, centromeric region)|go:"GO:0003712"(transcription coregulator activity)|go:"GO:0004402"(histone acetyltransferase activity)|go:"GO:0005634"(nucleus)|go:"GO:0005671"(obsolete Ada2/Gcn5/Ada3 transcription activator complex)|go:"GO:0005829"(cytosol)|go:"GO:0006325"(chromatin organization)|go:"GO:0006338"(chromatin remodeling)|go:"GO:0010484"(H3 histone acetyltransferase activity)|go:"GO:0016573"(histone acetylation)|go:"GO:0032968"(positive regulation of transcription elongation from RNA polymerase II promoter)|go:"GO:0046695"("SLIK (SAGA-like) complex")|go:"GO:0070577"(lysine-acetylated histone binding)|go:"GO:0140068"(histone crotonyltransferase activity)|interpro:IPR000182(GCN5-related N-acetyltransferase)|interpro:IPR001487(Bromodomain)|interpro:IPR016181(Acyl-CoA N-acyltransferase)|interpro:IPR018359|interpro:IPR036427|interpro:IPR037800|rcsb pdb:1E6I|rcsb pdb:1YGH|rcsb pdb:6CW2|rcsb pdb:6CW3|reactome:R-SCE-5689880|reactome:R-SCE-9018519	-	crc64:083B63624669244F	crc64:3200730DDC7EF70D	figure legend:f5d|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	-	2015/05/19	2015/05/19	rogid:U3k+xA1SuVJRX240wH99s5ijrN0559292	rogid:Y8SHidiW4dJo0oxEDksur8MC/L0559292	rigid:xYh8U/N3WbiIlK5YW3xt1jD5MSM	false	myc tag:?-?	-	-	-	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)
uniprotkb:P35177	uniprotkb:Q03761	intact:EBI-17958|uniprotkb:D6VQ80|ensemblfungi:YBR081C	intact:EBI-35097|uniprotkb:D6VSC8|ensemblfungi:YDR145W	psi-mi:spt7_yeast(display_long)|uniprotkb:YBR0739(orf name)|uniprotkb:YBR081C(locus name)|uniprotkb:SPT7(gene name)|psi-mi:SPT7(display_short)	psi-mi:taf12_yeast(display_long)|uniprotkb:TAF12(gene name)|psi-mi:TAF12(display_short)|uniprotkb:TAF61(gene name synonym)|uniprotkb:TAF68(gene name synonym)|uniprotkb:YDR145W(locus name)|uniprotkb:YD8358.02(orf name)|uniprotkb:TBP-associated factor 12(gene name synonym)|uniprotkb:TBP-associated factor 61 kDa(gene name synonym)|uniprotkb:TAFII-61(gene name synonym)|uniprotkb:TAFII-68(gene name synonym)	psi-mi:"MI:0007"(anti tag coimmunoprecipitation)	Saint et al. (2014)	pubmed:24550006|imex:IM-24168	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-10821522|imex:IM-24168-3	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_009637.1|sgd:S000000285|ensemblfungi:YBR081C(gene)|ensemblfungi:YBR081C_mRNA(transcript)|go:"GO:0000124"(SAGA complex)|go:"GO:0000747"(conjugation with cellular fusion)|go:"GO:0005198"(structural molecule activity)|go:"GO:0005739"(mitochondrion)|go:"GO:0006325"(chromatin organization)|go:"GO:0006357"(regulation of transcription by RNA polymerase II)|go:"GO:0016573"(histone acetylation)|go:"GO:0034622"|go:"GO:0046695"("SLIK (SAGA-like) complex")|go:"GO:0046982"(protein heterodimerization activity)|interpro:IPR001487(Bromodomain)|interpro:IPR009072(Histone-fold)|interpro:IPR018359|interpro:IPR036427|interpro:IPR037782|rcsb pdb:6T9I|rcsb pdb:6T9K|dip:DIP-771N|mint:P35177	refseq:NP_010429.1|sgd:S000002552|mint:Q03761|dip:DIP-1302N|ensemblfungi:YDR145W(gene)|ensemblfungi:YDR145W_mRNA(transcript)|go:"GO:0000124"(SAGA complex)|go:"GO:0003677"(DNA binding)|go:"GO:0003682"(chromatin binding)|go:"GO:0005669"(transcription factor TFIID complex)|go:"GO:0006325"(chromatin organization)|go:"GO:0006366"(transcription by RNA polymerase II)|go:"GO:0016573"(histone acetylation)|go:"GO:0017025"(TBP-class protein binding)|go:"GO:0042802"(identical protein binding)|go:"GO:0046695"("SLIK (SAGA-like) complex")|go:"GO:0046982"(protein heterodimerization activity)|go:"GO:0051123"(RNA polymerase II preinitiation complex assembly)|go:"GO:0060090"(molecular adaptor activity)|go:"GO:0061629"(RNA polymerase II-specific DNA-binding transcription factor binding)|interpro:IPR003228(Transcription initiation factor TFIID)|interpro:IPR009072(Histone-fold)|interpro:IPR037794|rcsb pdb:6T9I|rcsb pdb:6T9J|rcsb pdb:6T9K|reactome:R-SCE-674695|reactome:R-SCE-73776|reactome:R-SCE-73779|reactome:R-SCE-75953|reactome:R-SCE-76042	-	crc64:083B63624669244F	crc64:A8FC84E46F8370D3	figure legend:f5d|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	-	2015/05/19	2015/05/19	rogid:U3k+xA1SuVJRX240wH99s5ijrN0559292	rogid:DCTuNehYD4fhp7PRgtHZyNIDbH4559292	rigid:xYh8U/N3WbiIlK5YW3xt1jD5MSM	false	myc tag:?-?	-	-	-	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)
uniprotkb:P35177	uniprotkb:P38129	intact:EBI-17958|uniprotkb:D6VQ80|ensemblfungi:YBR081C	intact:EBI-18868|ensemblfungi:YBR198C|uniprotkb:D6VQJ4	psi-mi:spt7_yeast(display_long)|uniprotkb:YBR0739(orf name)|uniprotkb:YBR081C(locus name)|uniprotkb:SPT7(gene name)|psi-mi:SPT7(display_short)	psi-mi:taf5_yeast(display_long)|uniprotkb:TAF5(gene name)|psi-mi:TAF5(display_short)|uniprotkb:TAF90(gene name synonym)|uniprotkb:YBR198C(locus name)|uniprotkb:YBR1410(orf name)|uniprotkb:TBP-associated factor 5(gene name synonym)|uniprotkb:TBP-associated factor 90 kDa(gene name synonym)|uniprotkb:TAFII-90(gene name synonym)	psi-mi:"MI:0007"(anti tag coimmunoprecipitation)	Saint et al. (2014)	pubmed:24550006|imex:IM-24168	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-10821522|imex:IM-24168-3	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_009637.1|sgd:S000000285|ensemblfungi:YBR081C(gene)|ensemblfungi:YBR081C_mRNA(transcript)|go:"GO:0000124"(SAGA complex)|go:"GO:0000747"(conjugation with cellular fusion)|go:"GO:0005198"(structural molecule activity)|go:"GO:0005739"(mitochondrion)|go:"GO:0006325"(chromatin organization)|go:"GO:0006357"(regulation of transcription by RNA polymerase II)|go:"GO:0016573"(histone acetylation)|go:"GO:0034622"|go:"GO:0046695"("SLIK (SAGA-like) complex")|go:"GO:0046982"(protein heterodimerization activity)|interpro:IPR001487(Bromodomain)|interpro:IPR009072(Histone-fold)|interpro:IPR018359|interpro:IPR036427|interpro:IPR037782|rcsb pdb:6T9I|rcsb pdb:6T9K|dip:DIP-771N|mint:P35177	ensemblfungi:YBR198C(gene)|ensemblfungi:YBR198C_mRNA(transcript)|go:"GO:0000124"(SAGA complex)|go:"GO:0003682"(chromatin binding)|go:"GO:0005669"(transcription factor TFIID complex)|go:"GO:0006325"(chromatin organization)|go:"GO:0006355"(regulation of transcription, DNA-templated)|go:"GO:0006366"(transcription by RNA polymerase II)|go:"GO:0006367"(transcription initiation from RNA polymerase II promoter)|go:"GO:0016573"(histone acetylation)|go:"GO:0042802"(identical protein binding)|go:"GO:0043130"(ubiquitin binding)|go:"GO:0043966"(histone H3 acetylation)|go:"GO:0046695"("SLIK (SAGA-like) complex")|refseq:NP_009757.1|sgd:S000000402|go:"GO:0060090"(molecular adaptor activity)|interpro:IPR001680(WD40 repeat)|interpro:IPR006594(LisH dimerisation motif)|interpro:IPR007582(WD40 associated region in TFIID subunit)|interpro:IPR015943(WD40/YVTN repeat-like)|interpro:IPR019775|interpro:IPR020472|interpro:IPR036322|interpro:IPR037264|interpro:IPR037783|rcsb pdb:2J49|rcsb pdb:6T9I|rcsb pdb:6T9K|reactome:R-SCE-674695|reactome:R-SCE-6807505|reactome:R-SCE-73776|reactome:R-SCE-73779|reactome:R-SCE-75953|reactome:R-SCE-76042|go:"GO:0051123"(RNA polymerase II preinitiation complex assembly)|dip:DIP-740N|mint:P38129	-	crc64:083B63624669244F	crc64:B42315B8C752D0B6	figure legend:f5d|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	-	2015/05/19	2015/05/19	rogid:U3k+xA1SuVJRX240wH99s5ijrN0559292	rogid:QA3hzlcvZh26ejkE7QNXNFP/l2Y559292	rigid:xYh8U/N3WbiIlK5YW3xt1jD5MSM	false	myc tag:?-?	-	-	-	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)
uniprotkb:P35177	uniprotkb:P53040	intact:EBI-17958|uniprotkb:D6VQ80|ensemblfungi:YBR081C	intact:EBI-18876|uniprotkb:D6VU35|ensemblfungi:YGL112C	psi-mi:spt7_yeast(display_long)|uniprotkb:YBR0739(orf name)|uniprotkb:YBR081C(locus name)|uniprotkb:SPT7(gene name)|psi-mi:SPT7(display_short)	psi-mi:taf6_yeast(display_long)|uniprotkb:TAF6(gene name)|psi-mi:TAF6(display_short)|uniprotkb:TAF60(gene name synonym)|uniprotkb:YGL112C(locus name)|uniprotkb:G2985(orf name)|uniprotkb:TBP-associated factor 6(gene name synonym)|uniprotkb:TBP-associated factor 60 kDa(gene name synonym)	psi-mi:"MI:0007"(anti tag coimmunoprecipitation)	Saint et al. (2014)	pubmed:24550006|imex:IM-24168	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-10821522|imex:IM-24168-3	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_009637.1|sgd:S000000285|ensemblfungi:YBR081C(gene)|ensemblfungi:YBR081C_mRNA(transcript)|go:"GO:0000124"(SAGA complex)|go:"GO:0000747"(conjugation with cellular fusion)|go:"GO:0005198"(structural molecule activity)|go:"GO:0005739"(mitochondrion)|go:"GO:0006325"(chromatin organization)|go:"GO:0006357"(regulation of transcription by RNA polymerase II)|go:"GO:0016573"(histone acetylation)|go:"GO:0034622"|go:"GO:0046695"("SLIK (SAGA-like) complex")|go:"GO:0046982"(protein heterodimerization activity)|interpro:IPR001487(Bromodomain)|interpro:IPR009072(Histone-fold)|interpro:IPR018359|interpro:IPR036427|interpro:IPR037782|rcsb pdb:6T9I|rcsb pdb:6T9K|dip:DIP-771N|mint:P35177	refseq:NP_011403.1|sgd:S000003080|interpro:IPR004823("TATA box binding protein associated factor (TAF)")|interpro:IPR009072(Histone-fold)|interpro:IPR011442(Protein of unknown function DUF1546)|go:"GO:2000144"(positive regulation of DNA-templated transcription, initiation)|interpro:IPR016024(Armadillo-type fold)|interpro:IPR037796|rcsb pdb:6T9I|rcsb pdb:6T9K|reactome:R-SCE-674695|reactome:R-SCE-6807505|reactome:R-SCE-73776|reactome:R-SCE-73779|reactome:R-SCE-75953|reactome:R-SCE-76042|ensemblfungi:YGL112C(gene)|ensemblfungi:YGL112C_mRNA(transcript)|go:"GO:0000124"(SAGA complex)|go:"GO:0003682"(chromatin binding)|go:"GO:0003713"(transcription coactivator activity)|go:"GO:0005634"(nucleus)|go:"GO:0005669"(transcription factor TFIID complex)|go:"GO:0005829"(cytosol)|go:"GO:0006325"(chromatin organization)|go:"GO:0006366"(transcription by RNA polymerase II)|go:"GO:0006367"(transcription initiation from RNA polymerase II promoter)|go:"GO:0016251"(RNA polymerase II general transcription initiation factor activity)|go:"GO:0016573"(histone acetylation)|go:"GO:0042802"(identical protein binding)|go:"GO:0045944"(positive regulation of transcription by RNA polymerase II)|go:"GO:0046695"("SLIK (SAGA-like) complex")|go:"GO:0046982"(protein heterodimerization activity)|go:"GO:0051123"(RNA polymerase II preinitiation complex assembly)|go:"GO:0060090"(molecular adaptor activity)|go:"GO:0061629"(RNA polymerase II-specific DNA-binding transcription factor binding)|dip:DIP-981N|mint:P53040	-	crc64:083B63624669244F	crc64:14B590E02C16F289	figure legend:f5d|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	-	2015/05/19	2015/05/19	rogid:U3k+xA1SuVJRX240wH99s5ijrN0559292	rogid:qmzBE/xAfQskdJqoI8oIqAIa3+A559292	rigid:xYh8U/N3WbiIlK5YW3xt1jD5MSM	false	myc tag:?-?	-	-	-	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)
uniprotkb:P35177	uniprotkb:Q05027	intact:EBI-17958|uniprotkb:D6VQ80|ensemblfungi:YBR081C	intact:EBI-27500|uniprotkb:D6W062|ensemblfungi:YMR236W	psi-mi:spt7_yeast(display_long)|uniprotkb:YBR0739(orf name)|uniprotkb:YBR081C(locus name)|uniprotkb:SPT7(gene name)|psi-mi:SPT7(display_short)	psi-mi:taf9_yeast(display_long)|uniprotkb:TAF9(gene name)|psi-mi:TAF9(display_short)|uniprotkb:TAF17(gene name synonym)|uniprotkb:YMR236W(locus name)|uniprotkb:YM9959.18(orf name)|uniprotkb:TBP-associated factor 9(gene name synonym)|uniprotkb:TBP-associated factor 17 kDa(gene name synonym)|uniprotkb:TAFII-17(gene name synonym)|uniprotkb:TAFII20(gene name synonym)	psi-mi:"MI:0007"(anti tag coimmunoprecipitation)	Saint et al. (2014)	pubmed:24550006|imex:IM-24168	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-10821522|imex:IM-24168-3	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_009637.1|sgd:S000000285|ensemblfungi:YBR081C(gene)|ensemblfungi:YBR081C_mRNA(transcript)|go:"GO:0000124"(SAGA complex)|go:"GO:0000747"(conjugation with cellular fusion)|go:"GO:0005198"(structural molecule activity)|go:"GO:0005739"(mitochondrion)|go:"GO:0006325"(chromatin organization)|go:"GO:0006357"(regulation of transcription by RNA polymerase II)|go:"GO:0016573"(histone acetylation)|go:"GO:0034622"|go:"GO:0046695"("SLIK (SAGA-like) complex")|go:"GO:0046982"(protein heterodimerization activity)|interpro:IPR001487(Bromodomain)|interpro:IPR009072(Histone-fold)|interpro:IPR018359|interpro:IPR036427|interpro:IPR037782|rcsb pdb:6T9I|rcsb pdb:6T9K|dip:DIP-771N|mint:P35177	mint:Q05027|refseq:NP_013963.1|sgd:S000004849|dip:DIP-925N|ensemblfungi:YMR236W(gene)|ensemblfungi:YMR236W_mRNA(transcript)|go:"GO:0000124"(SAGA complex)|go:"GO:0003682"(chromatin binding)|go:"GO:0003713"(transcription coactivator activity)|go:"GO:0005669"(transcription factor TFIID complex)|go:"GO:0006325"(chromatin organization)|go:"GO:0006366"(transcription by RNA polymerase II)|go:"GO:0016573"(histone acetylation)|go:"GO:0042802"(identical protein binding)|go:"GO:0043966"(histone H3 acetylation)|go:"GO:0045944"(positive regulation of transcription by RNA polymerase II)|go:"GO:0046695"("SLIK (SAGA-like) complex")|go:"GO:0046982"(protein heterodimerization activity)|go:"GO:0051123"(RNA polymerase II preinitiation complex assembly)|go:"GO:0060090"(molecular adaptor activity)|interpro:IPR003162(Transcription factor TAFII-31)|interpro:IPR009072(Histone-fold)|rcsb pdb:6T9I|rcsb pdb:6T9K|reactome:R-SCE-5689880|reactome:R-SCE-674695|reactome:R-SCE-6807505|reactome:R-SCE-73776|reactome:R-SCE-73779|reactome:R-SCE-75953|reactome:R-SCE-76042	-	crc64:083B63624669244F	crc64:D1C97EDDCA468EC5	figure legend:f5d|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	-	2015/05/19	2015/05/19	rogid:U3k+xA1SuVJRX240wH99s5ijrN0559292	rogid:3+S7ozz3r1gCMvBUUfkGpeTCy/k559292	rigid:xYh8U/N3WbiIlK5YW3xt1jD5MSM	false	myc tag:?-?	binding-associated region:1-131|ha tag:?-?	-	-	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)
uniprotkb:P35177	uniprotkb:P38811	intact:EBI-17958|uniprotkb:D6VQ80|ensemblfungi:YBR081C	intact:EBI-24638|uniprotkb:D3DL49|ensemblfungi:YHR099W	psi-mi:spt7_yeast(display_long)|uniprotkb:YBR0739(orf name)|uniprotkb:YBR081C(locus name)|uniprotkb:SPT7(gene name)|psi-mi:SPT7(display_short)	psi-mi:tra1_yeast(display_long)|uniprotkb:TRA1(gene name)|psi-mi:TRA1(display_short)|uniprotkb:p400 kDa component of SAGA(gene name synonym)|uniprotkb:YHR099W(locus name)	psi-mi:"MI:0007"(anti tag coimmunoprecipitation)	Saint et al. (2014)	pubmed:24550006|imex:IM-24168	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-10821522|imex:IM-24168-3	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_009637.1|sgd:S000000285|ensemblfungi:YBR081C(gene)|ensemblfungi:YBR081C_mRNA(transcript)|go:"GO:0000124"(SAGA complex)|go:"GO:0000747"(conjugation with cellular fusion)|go:"GO:0005198"(structural molecule activity)|go:"GO:0005739"(mitochondrion)|go:"GO:0006325"(chromatin organization)|go:"GO:0006357"(regulation of transcription by RNA polymerase II)|go:"GO:0016573"(histone acetylation)|go:"GO:0034622"|go:"GO:0046695"("SLIK (SAGA-like) complex")|go:"GO:0046982"(protein heterodimerization activity)|interpro:IPR001487(Bromodomain)|interpro:IPR009072(Histone-fold)|interpro:IPR018359|interpro:IPR036427|interpro:IPR037782|rcsb pdb:6T9I|rcsb pdb:6T9K|dip:DIP-771N|mint:P35177	refseq:NP_011967.1|sgd:S000001141|dip:DIP-805N|mint:P38811|ensemblfungi:YHR099W(gene)|ensemblfungi:YHR099W_mRNA(transcript)|go:"GO:0000124"(SAGA complex)|go:"GO:0003712"(transcription coregulator activity)|go:"GO:0005634"(nucleus)|go:"GO:0006281"(DNA repair)|go:"GO:0006355"(regulation of transcription, DNA-templated)|go:"GO:0006468"(protein phosphorylation)|go:"GO:0016301"(kinase activity)|go:"GO:0016573"(histone acetylation)|go:"GO:0035267"(NuA4 histone acetyltransferase complex)|go:"GO:0045944"(positive regulation of transcription by RNA polymerase II)|go:"GO:0046695"("SLIK (SAGA-like) complex")|go:"GO:0070209"(ASTRA complex)|interpro:IPR000403(Phosphatidylinositol 3- and 4-kinase, catalytic)|interpro:IPR003151(PIK-related kinase, FAT)|interpro:IPR003152(PIK-related kinase, FATC)|interpro:IPR011009(Protein kinase-like)|interpro:IPR014009(PIK-related kinase)|interpro:IPR016024(Armadillo-type fold)|interpro:IPR033317|interpro:IPR036940|rcsb pdb:5OJS|rcsb pdb:6IG9|rcsb pdb:6T9I|rcsb pdb:6T9J	-	crc64:083B63624669244F	crc64:AE3588676F5D5777	figure legend:f5d|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	-	2015/05/19	2015/05/19	rogid:U3k+xA1SuVJRX240wH99s5ijrN0559292	rogid:B/rkvJoIvK1Xi2SoNtFnFOdcp2c559292	rigid:xYh8U/N3WbiIlK5YW3xt1jD5MSM	false	myc tag:?-?	-	-	-	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)
uniprotkb:P03069	uniprotkb:P13393	intact:EBI-7450|uniprotkb:P03068|uniprotkb:Q70D88|uniprotkb:Q70D91|uniprotkb:Q70D96|uniprotkb:Q70D99|uniprotkb:Q70DA0|uniprotkb:Q96UT3|uniprotkb:D3DLN9|ensemblfungi:YEL009C	intact:EBI-19129|uniprotkb:D3DM55|ensemblfungi:YER148W	psi-mi:gcn4_yeast(display_long)|uniprotkb:ARG9(gene name synonym)|uniprotkb:GCN4(gene name)|psi-mi:GCN4(display_short)|uniprotkb:AAS3(gene name synonym)|uniprotkb:Amino acid biosynthesis regulatory protein(gene name synonym)|uniprotkb:AAS101(gene name synonym)|uniprotkb:General control protein GCN4(gene name synonym)|uniprotkb:YEL009C(locus name)	psi-mi:tbp_yeast(display_long)|uniprotkb:YER148W(locus name)|uniprotkb:SPT15(gene name)|psi-mi:SPT15(display_short)|uniprotkb:BTF1(gene name synonym)|uniprotkb:TBP1(gene name synonym)|uniprotkb:TATA-box factor(gene name synonym)|uniprotkb:TATA-binding factor(gene name synonym)|uniprotkb:TATA sequence-binding protein(gene name synonym)|uniprotkb:Transcription factor D(gene name synonym)|uniprotkb:Transcription initiation factor TFIID TBP subunit(gene name synonym)	psi-mi:"MI:0096"(pull down)	Saint et al. (2014)	pubmed:24550006|imex:IM-24168	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-10823079|imex:IM-24168-4	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	sgd:S000000735|refseq:NP_010907.3|mint:P03069|dip:DIP-591N|ensemblfungi:YEL009C(gene)|ensemblfungi:YEL009C_mRNA(transcript)|go:"GO:0000122"(negative regulation of transcription by RNA polymerase II)|go:"GO:0000978"(RNA polymerase II cis-regulatory region sequence-specific DNA binding)|go:"GO:0000981"(DNA-binding transcription factor activity, RNA polymerase II-specific)|go:"GO:0001080"(nitrogen catabolite activation of transcription from RNA polymerase II promoter)|go:"GO:0001228"(DNA-binding transcription activator activity, RNA polymerase II-specific)|go:"GO:0003682"(chromatin binding)|go:"GO:0003700"(DNA-binding transcription factor activity)|go:"GO:0005634"(nucleus)|go:"GO:0005667"(transcription regulator complex)|go:"GO:0006357"(regulation of transcription by RNA polymerase II)|go:"GO:0006914"(autophagy)|go:"GO:0008652"(cellular amino acid biosynthetic process)|go:"GO:0010508"(positive regulation of autophagy)|go:"GO:0010691"(negative regulation of ribosomal protein gene transcription from RNA polymerase II promoter in response to nutrient levels)|go:"GO:0035556"(intracellular signal transduction)|go:"GO:0036278"(positive regulation of transcription from RNA polymerase II promoter in response to nitrogen starvation)|go:"GO:0042802"(identical protein binding)|go:"GO:0043565"(sequence-specific DNA binding)|go:"GO:0043621"(protein self-association)|go:"GO:0045899"(positive regulation of RNA polymerase II transcription preinitiation complex assembly)|go:"GO:0045944"(positive regulation of transcription by RNA polymerase II)|go:"GO:0060261"(positive regulation of transcription initiation from RNA polymerase II promoter)|go:"GO:0061412"(positive regulation of transcription from RNA polymerase II promoter in response to amino acid starvation)|go:"GO:0061629"(RNA polymerase II-specific DNA-binding transcription factor binding)|go:"GO:0090575"(RNA polymerase II transcription regulator complex)|go:"GO:1903833"(positive regulation of cellular response to amino acid starvation)|go:"GO:1990139"(protein localization to nuclear periphery)|go:"GO:1990928"(response to amino acid starvation)|interpro:IPR004827("Basic-leucine zipper (bZIP) transcription factor")|rcsb pdb:1CE9|rcsb pdb:1DGC|rcsb pdb:1ENV|rcsb pdb:1FAV|rcsb pdb:1FMH|rcsb pdb:1GCL|rcsb pdb:1GCM|rcsb pdb:1GK6|rcsb pdb:1GZL|rcsb pdb:1IHQ|rcsb pdb:1IJ0|rcsb pdb:1IJ1|rcsb pdb:1IJ2|rcsb pdb:1IJ3|rcsb pdb:1KQL|rcsb pdb:1LD4|rcsb pdb:1LLM|rcsb pdb:1NKN|rcsb pdb:1PIQ|rcsb pdb:1RB4|rcsb pdb:1RB5|rcsb pdb:1RB6|rcsb pdb:1SWI|rcsb pdb:1TMZ|rcsb pdb:1UNT|rcsb pdb:1UNU|rcsb pdb:1UNV|rcsb pdb:1UNW|rcsb pdb:1UNX|rcsb pdb:1UNY|rcsb pdb:1UNZ|rcsb pdb:1UO0|rcsb pdb:1UO1|rcsb pdb:1UO2|rcsb pdb:1UO3|rcsb pdb:1UO4|rcsb pdb:1UO5|rcsb pdb:1W5G|rcsb pdb:1W5H|rcsb pdb:1W5I|rcsb pdb:1W5J|rcsb pdb:1W5K|rcsb pdb:1W5L|rcsb pdb:1YSA|rcsb pdb:1ZII|rcsb pdb:1ZIJ|rcsb pdb:1ZIK|rcsb pdb:1ZIL|rcsb pdb:1ZIM|rcsb pdb:1ZTA|rcsb pdb:2AHP|rcsb pdb:2B1F|rcsb pdb:2B22|rcsb pdb:2BNI|rcsb pdb:2CCE|rcsb pdb:2CCF|rcsb pdb:2CCN|rcsb pdb:2D3E|rcsb pdb:2DGC|rcsb pdb:2EFR|rcsb pdb:2EFS|rcsb pdb:2G9J|rcsb pdb:2HY6|rcsb pdb:2IPZ|rcsb pdb:2K8X|rcsb pdb:2LPB|rcsb pdb:2N9B|rcsb pdb:2NRN|rcsb pdb:2O7H|rcsb pdb:2OVN|rcsb pdb:2VKY|rcsb pdb:2VNL|rcsb pdb:2WG5|rcsb pdb:2WG6|rcsb pdb:2WPY|rcsb pdb:2WPZ|rcsb pdb:2WQ0|rcsb pdb:2WQ1|rcsb pdb:2WQ2|rcsb pdb:2WQ3|rcsb pdb:2YNY|rcsb pdb:2YNZ|rcsb pdb:2YO0|rcsb pdb:2YO1|rcsb pdb:2YO2|rcsb pdb:2YO3|rcsb pdb:2Z5H|rcsb pdb:2Z5I|rcsb pdb:2ZTA|rcsb pdb:3AZD|rcsb pdb:3BAS|rcsb pdb:3BAT|rcsb pdb:3CK4|rcsb pdb:3CRP|rcsb pdb:3G9R|rcsb pdb:3GJP|rcsb pdb:3I1G|rcsb pdb:3I5C|rcsb pdb:3K7Z|rcsb pdb:3M48|rcsb pdb:3P8M|rcsb pdb:3ZMF|rcsb pdb:4C46|rcsb pdb:4DMD|rcsb pdb:4G2K|rcsb pdb:4HU5|rcsb pdb:4HU6|rcsb pdb:4NIZ|rcsb pdb:4NJ0|rcsb pdb:4NJ1|rcsb pdb:4NJ2|rcsb pdb:4OWI|rcsb pdb:4TL1|rcsb pdb:5APP|rcsb pdb:5APQ|rcsb pdb:5APS|rcsb pdb:5APT|rcsb pdb:5APU|rcsb pdb:5APV|rcsb pdb:5APW|rcsb pdb:5APX|rcsb pdb:5APY|rcsb pdb:5APZ|rcsb pdb:5IEW|rcsb pdb:5IIR|rcsb pdb:5IIV|rcsb pdb:5KHT|rcsb pdb:6E52|rcsb pdb:6E95|rcsb pdb:6H9M|rcsb pdb:6HN4|rcsb pdb:6HN5|rcsb pdb:6O2E|rcsb pdb:6O2F|rcsb pdb:6PSA|rcsb pdb:6UT2|rcsb pdb:6VWG|rcsb pdb:6VWH|rcsb pdb:6VWI|rcsb pdb:6VWJ|reactome:R-SCE-2559580|reactome:R-SCE-2871796|reactome:R-SCE-450341	sgd:S000000950|ensemblfungi:YER148W(gene)|ensemblfungi:YER148W_mRNA(transcript)|go:"GO:0000126"(transcription factor TFIIIB complex)|go:"GO:0000979"(RNA polymerase II core promoter sequence-specific DNA binding)|go:"GO:0000995"(RNA polymerase III general transcription initiation factor activity)|go:"GO:0001016"(RNA polymerase III transcription regulatory region sequence-specific DNA binding)|go:"GO:0001092"(TFIIA-class transcription factor complex binding)|go:"GO:0001179"(RNA polymerase I general transcription initiation factor binding)|go:"GO:0001188"(RNA polymerase I preinitiation complex assembly)|go:"GO:0003682"(chromatin binding)|go:"GO:0005634"(nucleus)|go:"GO:0005654"(nucleoplasm)|go:"GO:0005669"(transcription factor TFIID complex)|go:"GO:0005672"(transcription factor TFIIA complex)|go:"GO:0006352"(DNA-templated transcription, initiation)|go:"GO:0008301"(DNA binding, bending)|go:"GO:0016251"(RNA polymerase II general transcription initiation factor activity)|go:"GO:0032991"(protein-containing complex)|go:"GO:0042790"(nucleolar large rRNA transcription by RNA polymerase I)|go:"GO:0051123"(RNA polymerase II preinitiation complex assembly)|go:"GO:0061629"(RNA polymerase II-specific DNA-binding transcription factor binding)|go:"GO:0070893"(transposon integration)|go:"GO:0070898"(RNA polymerase III preinitiation complex assembly)|go:"GO:0097718"(disordered domain specific binding)|go:"GO:0140223"(general transcription initiation factor activity)|go:"GO:0140297"(DNA-binding transcription factor binding)|interpro:IPR000814(TATA-box binding)|interpro:IPR012295(Beta2-adaptin/TATA-box binding, C-terminal)|interpro:IPR030491|interpro:IPR033710|rcsb pdb:1NGM|rcsb pdb:1NH2|rcsb pdb:1RM1|rcsb pdb:1TBA|rcsb pdb:1TBP|rcsb pdb:1YTB|rcsb pdb:1YTF|rcsb pdb:4B0A|rcsb pdb:4V1N|rcsb pdb:4V1O|rcsb pdb:5FMF|rcsb pdb:5FYW|rcsb pdb:5FZ5|rcsb pdb:5OQJ|rcsb pdb:5OQM|rcsb pdb:5SVA|rcsb pdb:6CNB|rcsb pdb:6CNC|rcsb pdb:6CND|rcsb pdb:6CNF|rcsb pdb:6E16|rcsb pdb:6E24|rcsb pdb:6EU0|rcsb pdb:6F40|rcsb pdb:6F41|rcsb pdb:6F42|rcsb pdb:6F44|rcsb pdb:6GYK|rcsb pdb:6GYL|rcsb pdb:6GYM|rcsb pdb:6TBM|rcsb pdb:7O4I|rcsb pdb:7O4J|rcsb pdb:7O72|rcsb pdb:7O73|rcsb pdb:7O75|reactome:R-SCE-674695|reactome:R-SCE-6807505|reactome:R-SCE-73772|reactome:R-SCE-73776|reactome:R-SCE-73779|reactome:R-SCE-75953|reactome:R-SCE-76042|reactome:R-SCE-76061|reactome:R-SCE-76066|reactome:R-SCE-9018519|refseq:NP_011075.3|dip:DIP-44N|mint:P13393	-	crc64:2ED1B8E35D509578	crc64:50365FFF1D3718CE	figure legend:f5e|comment:"we tested whether the lack of the Taf9 CRD  impaired the interaction of Gcn4 with the SAGA complex. GST  pulldown assays (9, 23) using GST-Gcn4 showed interaction of  Spt7, Gcn5, Tra1, Taf12, and Taf9 from the TAF9-HA3 extract in a  manner dependent on the WT Gcn4 activation domain (Fig. 5E).  As expected from our previous study (9), the SWI/SNF subunit  Snf6, used as a control here, was specifically pulled down. Whereas  no interaction with Taf1 was seen, interaction with TBP did not  show specificity for the Gcn4 activation domain, as observed previously  (9, 23). The pulldown assays conducted from the tCRD2-  HA3 extract also showed the pulldown of the same SAGA subunits,  albeit with reduced recovery of the Gcn5 subunit (Fig. 5E).  Importantly, the interaction of Gcn4 with Tra1 and Taf12 was not  impaired in the tCRD2-HA3 extract (Fig. 5E). Together, these results  indicate that the interactions of the Gcn4 activation domain  with the mutant SAGA complex are largely intact in vitro."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2015/05/19	2015/05/21	rogid:MFj9JcYccWKTayvnuaN95djsHd0559292	rogid:fVZPztzKW8tuxDYYpZ7ZTFlq3Do559292	rigid:h7fKB+ckA1LiSRUrDgYoc5WOKhE	false	glutathione s tranferase tag:?-?	-	-	-	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)
uniprotkb:P03069	uniprotkb:P18888	intact:EBI-7450|uniprotkb:P03068|uniprotkb:Q70D88|uniprotkb:Q70D91|uniprotkb:Q70D96|uniprotkb:Q70D99|uniprotkb:Q70DA0|uniprotkb:Q96UT3|uniprotkb:D3DLN9|ensemblfungi:YEL009C	intact:EBI-17550|uniprotkb:D3DKU3|ensemblfungi:YHL025W	psi-mi:gcn4_yeast(display_long)|uniprotkb:ARG9(gene name synonym)|uniprotkb:GCN4(gene name)|psi-mi:GCN4(display_short)|uniprotkb:AAS3(gene name synonym)|uniprotkb:Amino acid biosynthesis regulatory protein(gene name synonym)|uniprotkb:AAS101(gene name synonym)|uniprotkb:General control protein GCN4(gene name synonym)|uniprotkb:YEL009C(locus name)	psi-mi:snf6_yeast(display_long)|uniprotkb:SNF6(gene name)|psi-mi:SNF6(display_short)|uniprotkb:YHL025W(locus name)|uniprotkb:SWI/SNF complex component SNF6(gene name synonym)	psi-mi:"MI:0096"(pull down)	Saint et al. (2014)	pubmed:24550006|imex:IM-24168	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-10823079|imex:IM-24168-4	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	sgd:S000000735|refseq:NP_010907.3|mint:P03069|dip:DIP-591N|ensemblfungi:YEL009C(gene)|ensemblfungi:YEL009C_mRNA(transcript)|go:"GO:0000122"(negative regulation of transcription by RNA polymerase II)|go:"GO:0000978"(RNA polymerase II cis-regulatory region sequence-specific DNA binding)|go:"GO:0000981"(DNA-binding transcription factor activity, RNA polymerase II-specific)|go:"GO:0001080"(nitrogen catabolite activation of transcription from RNA polymerase II promoter)|go:"GO:0001228"(DNA-binding transcription activator activity, RNA polymerase II-specific)|go:"GO:0003682"(chromatin binding)|go:"GO:0003700"(DNA-binding transcription factor activity)|go:"GO:0005634"(nucleus)|go:"GO:0005667"(transcription regulator complex)|go:"GO:0006357"(regulation of transcription by RNA polymerase II)|go:"GO:0006914"(autophagy)|go:"GO:0008652"(cellular amino acid biosynthetic process)|go:"GO:0010508"(positive regulation of autophagy)|go:"GO:0010691"(negative regulation of ribosomal protein gene transcription from RNA polymerase II promoter in response to nutrient levels)|go:"GO:0035556"(intracellular signal transduction)|go:"GO:0036278"(positive regulation of transcription from RNA polymerase II promoter in response to nitrogen starvation)|go:"GO:0042802"(identical protein binding)|go:"GO:0043565"(sequence-specific DNA binding)|go:"GO:0043621"(protein self-association)|go:"GO:0045899"(positive regulation of RNA polymerase II transcription preinitiation complex assembly)|go:"GO:0045944"(positive regulation of transcription by RNA polymerase II)|go:"GO:0060261"(positive regulation of transcription initiation from RNA polymerase II promoter)|go:"GO:0061412"(positive regulation of transcription from RNA polymerase II promoter in response to amino acid starvation)|go:"GO:0061629"(RNA polymerase II-specific DNA-binding transcription factor binding)|go:"GO:0090575"(RNA polymerase II transcription regulator complex)|go:"GO:1903833"(positive regulation of cellular response to amino acid starvation)|go:"GO:1990139"(protein localization to nuclear periphery)|go:"GO:1990928"(response to amino acid starvation)|interpro:IPR004827("Basic-leucine zipper (bZIP) transcription factor")|rcsb pdb:1CE9|rcsb pdb:1DGC|rcsb pdb:1ENV|rcsb pdb:1FAV|rcsb pdb:1FMH|rcsb pdb:1GCL|rcsb pdb:1GCM|rcsb pdb:1GK6|rcsb pdb:1GZL|rcsb pdb:1IHQ|rcsb pdb:1IJ0|rcsb pdb:1IJ1|rcsb pdb:1IJ2|rcsb pdb:1IJ3|rcsb pdb:1KQL|rcsb pdb:1LD4|rcsb pdb:1LLM|rcsb pdb:1NKN|rcsb pdb:1PIQ|rcsb pdb:1RB4|rcsb pdb:1RB5|rcsb pdb:1RB6|rcsb pdb:1SWI|rcsb pdb:1TMZ|rcsb pdb:1UNT|rcsb pdb:1UNU|rcsb pdb:1UNV|rcsb pdb:1UNW|rcsb pdb:1UNX|rcsb pdb:1UNY|rcsb pdb:1UNZ|rcsb pdb:1UO0|rcsb pdb:1UO1|rcsb pdb:1UO2|rcsb pdb:1UO3|rcsb pdb:1UO4|rcsb pdb:1UO5|rcsb pdb:1W5G|rcsb pdb:1W5H|rcsb pdb:1W5I|rcsb pdb:1W5J|rcsb pdb:1W5K|rcsb pdb:1W5L|rcsb pdb:1YSA|rcsb pdb:1ZII|rcsb pdb:1ZIJ|rcsb pdb:1ZIK|rcsb pdb:1ZIL|rcsb pdb:1ZIM|rcsb pdb:1ZTA|rcsb pdb:2AHP|rcsb pdb:2B1F|rcsb pdb:2B22|rcsb pdb:2BNI|rcsb pdb:2CCE|rcsb pdb:2CCF|rcsb pdb:2CCN|rcsb pdb:2D3E|rcsb pdb:2DGC|rcsb pdb:2EFR|rcsb pdb:2EFS|rcsb pdb:2G9J|rcsb pdb:2HY6|rcsb pdb:2IPZ|rcsb pdb:2K8X|rcsb pdb:2LPB|rcsb pdb:2N9B|rcsb pdb:2NRN|rcsb pdb:2O7H|rcsb pdb:2OVN|rcsb pdb:2VKY|rcsb pdb:2VNL|rcsb pdb:2WG5|rcsb pdb:2WG6|rcsb pdb:2WPY|rcsb pdb:2WPZ|rcsb pdb:2WQ0|rcsb pdb:2WQ1|rcsb pdb:2WQ2|rcsb pdb:2WQ3|rcsb pdb:2YNY|rcsb pdb:2YNZ|rcsb pdb:2YO0|rcsb pdb:2YO1|rcsb pdb:2YO2|rcsb pdb:2YO3|rcsb pdb:2Z5H|rcsb pdb:2Z5I|rcsb pdb:2ZTA|rcsb pdb:3AZD|rcsb pdb:3BAS|rcsb pdb:3BAT|rcsb pdb:3CK4|rcsb pdb:3CRP|rcsb pdb:3G9R|rcsb pdb:3GJP|rcsb pdb:3I1G|rcsb pdb:3I5C|rcsb pdb:3K7Z|rcsb pdb:3M48|rcsb pdb:3P8M|rcsb pdb:3ZMF|rcsb pdb:4C46|rcsb pdb:4DMD|rcsb pdb:4G2K|rcsb pdb:4HU5|rcsb pdb:4HU6|rcsb pdb:4NIZ|rcsb pdb:4NJ0|rcsb pdb:4NJ1|rcsb pdb:4NJ2|rcsb pdb:4OWI|rcsb pdb:4TL1|rcsb pdb:5APP|rcsb pdb:5APQ|rcsb pdb:5APS|rcsb pdb:5APT|rcsb pdb:5APU|rcsb pdb:5APV|rcsb pdb:5APW|rcsb pdb:5APX|rcsb pdb:5APY|rcsb pdb:5APZ|rcsb pdb:5IEW|rcsb pdb:5IIR|rcsb pdb:5IIV|rcsb pdb:5KHT|rcsb pdb:6E52|rcsb pdb:6E95|rcsb pdb:6H9M|rcsb pdb:6HN4|rcsb pdb:6HN5|rcsb pdb:6O2E|rcsb pdb:6O2F|rcsb pdb:6PSA|rcsb pdb:6UT2|rcsb pdb:6VWG|rcsb pdb:6VWH|rcsb pdb:6VWI|rcsb pdb:6VWJ|reactome:R-SCE-2559580|reactome:R-SCE-2871796|reactome:R-SCE-450341	go:"GO:0005829"(cytosol)|refseq:NP_011838.1|sgd:S000001017|go:"GO:0006338"(chromatin remodeling)|go:"GO:0016514"(SWI/SNF complex)|go:"GO:0045943"(positive regulation of transcription by RNA polymerase I)|go:"GO:0045944"(positive regulation of transcription by RNA polymerase II)|rcsb pdb:6UXV|rcsb pdb:6UXW|rcsb pdb:7C4J|go:"GO:0006289"(nucleotide-excision repair)|ensemblfungi:YHL025W(gene)|ensemblfungi:YHL025W_mRNA(transcript)|go:"GO:0000182"(rDNA binding)|go:"GO:0000470"(maturation of LSU-rRNA)|go:"GO:0005634"(nucleus)|dip:DIP-1226N|mint:P18888	-	crc64:2ED1B8E35D509578	crc64:ED9EA9F313F9079F	figure legend:f5e|comment:"we tested whether the lack of the Taf9 CRD  impaired the interaction of Gcn4 with the SAGA complex. GST  pulldown assays (9, 23) using GST-Gcn4 showed interaction of  Spt7, Gcn5, Tra1, Taf12, and Taf9 from the TAF9-HA3 extract in a  manner dependent on the WT Gcn4 activation domain (Fig. 5E).  As expected from our previous study (9), the SWI/SNF subunit  Snf6, used as a control here, was specifically pulled down. Whereas  no interaction with Taf1 was seen, interaction with TBP did not  show specificity for the Gcn4 activation domain, as observed previously  (9, 23). The pulldown assays conducted from the tCRD2-  HA3 extract also showed the pulldown of the same SAGA subunits,  albeit with reduced recovery of the Gcn5 subunit (Fig. 5E).  Importantly, the interaction of Gcn4 with Tra1 and Taf12 was not  impaired in the tCRD2-HA3 extract (Fig. 5E). Together, these results  indicate that the interactions of the Gcn4 activation domain  with the mutant SAGA complex are largely intact in vitro."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2015/05/19	2015/05/21	rogid:MFj9JcYccWKTayvnuaN95djsHd0559292	rogid:OjRVJEikcmyHBr/qDYYwQ1TgP1c559292	rigid:h7fKB+ckA1LiSRUrDgYoc5WOKhE	false	glutathione s tranferase tag:?-?	-	-	-	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)
uniprotkb:P03069	uniprotkb:P38811	intact:EBI-7450|uniprotkb:P03068|uniprotkb:Q70D88|uniprotkb:Q70D91|uniprotkb:Q70D96|uniprotkb:Q70D99|uniprotkb:Q70DA0|uniprotkb:Q96UT3|uniprotkb:D3DLN9|ensemblfungi:YEL009C	intact:EBI-24638|uniprotkb:D3DL49|ensemblfungi:YHR099W	psi-mi:gcn4_yeast(display_long)|uniprotkb:ARG9(gene name synonym)|uniprotkb:GCN4(gene name)|psi-mi:GCN4(display_short)|uniprotkb:AAS3(gene name synonym)|uniprotkb:Amino acid biosynthesis regulatory protein(gene name synonym)|uniprotkb:AAS101(gene name synonym)|uniprotkb:General control protein GCN4(gene name synonym)|uniprotkb:YEL009C(locus name)	psi-mi:tra1_yeast(display_long)|uniprotkb:TRA1(gene name)|psi-mi:TRA1(display_short)|uniprotkb:p400 kDa component of SAGA(gene name synonym)|uniprotkb:YHR099W(locus name)	psi-mi:"MI:0096"(pull down)	Saint et al. (2014)	pubmed:24550006|imex:IM-24168	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-10823079|imex:IM-24168-4	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	sgd:S000000735|refseq:NP_010907.3|mint:P03069|dip:DIP-591N|ensemblfungi:YEL009C(gene)|ensemblfungi:YEL009C_mRNA(transcript)|go:"GO:0000122"(negative regulation of transcription by RNA polymerase II)|go:"GO:0000978"(RNA polymerase II cis-regulatory region sequence-specific DNA binding)|go:"GO:0000981"(DNA-binding transcription factor activity, RNA polymerase II-specific)|go:"GO:0001080"(nitrogen catabolite activation of transcription from RNA polymerase II promoter)|go:"GO:0001228"(DNA-binding transcription activator activity, RNA polymerase II-specific)|go:"GO:0003682"(chromatin binding)|go:"GO:0003700"(DNA-binding transcription factor activity)|go:"GO:0005634"(nucleus)|go:"GO:0005667"(transcription regulator complex)|go:"GO:0006357"(regulation of transcription by RNA polymerase II)|go:"GO:0006914"(autophagy)|go:"GO:0008652"(cellular amino acid biosynthetic process)|go:"GO:0010508"(positive regulation of autophagy)|go:"GO:0010691"(negative regulation of ribosomal protein gene transcription from RNA polymerase II promoter in response to nutrient levels)|go:"GO:0035556"(intracellular signal transduction)|go:"GO:0036278"(positive regulation of transcription from RNA polymerase II promoter in response to nitrogen starvation)|go:"GO:0042802"(identical protein binding)|go:"GO:0043565"(sequence-specific DNA binding)|go:"GO:0043621"(protein self-association)|go:"GO:0045899"(positive regulation of RNA polymerase II transcription preinitiation complex assembly)|go:"GO:0045944"(positive regulation of transcription by RNA polymerase II)|go:"GO:0060261"(positive regulation of transcription initiation from RNA polymerase II promoter)|go:"GO:0061412"(positive regulation of transcription from RNA polymerase II promoter in response to amino acid starvation)|go:"GO:0061629"(RNA polymerase II-specific DNA-binding transcription factor binding)|go:"GO:0090575"(RNA polymerase II transcription regulator complex)|go:"GO:1903833"(positive regulation of cellular response to amino acid starvation)|go:"GO:1990139"(protein localization to nuclear periphery)|go:"GO:1990928"(response to amino acid starvation)|interpro:IPR004827("Basic-leucine zipper (bZIP) transcription factor")|rcsb pdb:1CE9|rcsb pdb:1DGC|rcsb pdb:1ENV|rcsb pdb:1FAV|rcsb pdb:1FMH|rcsb pdb:1GCL|rcsb pdb:1GCM|rcsb pdb:1GK6|rcsb pdb:1GZL|rcsb pdb:1IHQ|rcsb pdb:1IJ0|rcsb pdb:1IJ1|rcsb pdb:1IJ2|rcsb pdb:1IJ3|rcsb pdb:1KQL|rcsb pdb:1LD4|rcsb pdb:1LLM|rcsb pdb:1NKN|rcsb pdb:1PIQ|rcsb pdb:1RB4|rcsb pdb:1RB5|rcsb pdb:1RB6|rcsb pdb:1SWI|rcsb pdb:1TMZ|rcsb pdb:1UNT|rcsb pdb:1UNU|rcsb pdb:1UNV|rcsb pdb:1UNW|rcsb pdb:1UNX|rcsb pdb:1UNY|rcsb pdb:1UNZ|rcsb pdb:1UO0|rcsb pdb:1UO1|rcsb pdb:1UO2|rcsb pdb:1UO3|rcsb pdb:1UO4|rcsb pdb:1UO5|rcsb pdb:1W5G|rcsb pdb:1W5H|rcsb pdb:1W5I|rcsb pdb:1W5J|rcsb pdb:1W5K|rcsb pdb:1W5L|rcsb pdb:1YSA|rcsb pdb:1ZII|rcsb pdb:1ZIJ|rcsb pdb:1ZIK|rcsb pdb:1ZIL|rcsb pdb:1ZIM|rcsb pdb:1ZTA|rcsb pdb:2AHP|rcsb pdb:2B1F|rcsb pdb:2B22|rcsb pdb:2BNI|rcsb pdb:2CCE|rcsb pdb:2CCF|rcsb pdb:2CCN|rcsb pdb:2D3E|rcsb pdb:2DGC|rcsb pdb:2EFR|rcsb pdb:2EFS|rcsb pdb:2G9J|rcsb pdb:2HY6|rcsb pdb:2IPZ|rcsb pdb:2K8X|rcsb pdb:2LPB|rcsb pdb:2N9B|rcsb pdb:2NRN|rcsb pdb:2O7H|rcsb pdb:2OVN|rcsb pdb:2VKY|rcsb pdb:2VNL|rcsb pdb:2WG5|rcsb pdb:2WG6|rcsb pdb:2WPY|rcsb pdb:2WPZ|rcsb pdb:2WQ0|rcsb pdb:2WQ1|rcsb pdb:2WQ2|rcsb pdb:2WQ3|rcsb pdb:2YNY|rcsb pdb:2YNZ|rcsb pdb:2YO0|rcsb pdb:2YO1|rcsb pdb:2YO2|rcsb pdb:2YO3|rcsb pdb:2Z5H|rcsb pdb:2Z5I|rcsb pdb:2ZTA|rcsb pdb:3AZD|rcsb pdb:3BAS|rcsb pdb:3BAT|rcsb pdb:3CK4|rcsb pdb:3CRP|rcsb pdb:3G9R|rcsb pdb:3GJP|rcsb pdb:3I1G|rcsb pdb:3I5C|rcsb pdb:3K7Z|rcsb pdb:3M48|rcsb pdb:3P8M|rcsb pdb:3ZMF|rcsb pdb:4C46|rcsb pdb:4DMD|rcsb pdb:4G2K|rcsb pdb:4HU5|rcsb pdb:4HU6|rcsb pdb:4NIZ|rcsb pdb:4NJ0|rcsb pdb:4NJ1|rcsb pdb:4NJ2|rcsb pdb:4OWI|rcsb pdb:4TL1|rcsb pdb:5APP|rcsb pdb:5APQ|rcsb pdb:5APS|rcsb pdb:5APT|rcsb pdb:5APU|rcsb pdb:5APV|rcsb pdb:5APW|rcsb pdb:5APX|rcsb pdb:5APY|rcsb pdb:5APZ|rcsb pdb:5IEW|rcsb pdb:5IIR|rcsb pdb:5IIV|rcsb pdb:5KHT|rcsb pdb:6E52|rcsb pdb:6E95|rcsb pdb:6H9M|rcsb pdb:6HN4|rcsb pdb:6HN5|rcsb pdb:6O2E|rcsb pdb:6O2F|rcsb pdb:6PSA|rcsb pdb:6UT2|rcsb pdb:6VWG|rcsb pdb:6VWH|rcsb pdb:6VWI|rcsb pdb:6VWJ|reactome:R-SCE-2559580|reactome:R-SCE-2871796|reactome:R-SCE-450341	refseq:NP_011967.1|sgd:S000001141|dip:DIP-805N|mint:P38811|ensemblfungi:YHR099W(gene)|ensemblfungi:YHR099W_mRNA(transcript)|go:"GO:0000124"(SAGA complex)|go:"GO:0003712"(transcription coregulator activity)|go:"GO:0005634"(nucleus)|go:"GO:0006281"(DNA repair)|go:"GO:0006355"(regulation of transcription, DNA-templated)|go:"GO:0006468"(protein phosphorylation)|go:"GO:0016301"(kinase activity)|go:"GO:0016573"(histone acetylation)|go:"GO:0035267"(NuA4 histone acetyltransferase complex)|go:"GO:0045944"(positive regulation of transcription by RNA polymerase II)|go:"GO:0046695"("SLIK (SAGA-like) complex")|go:"GO:0070209"(ASTRA complex)|interpro:IPR000403(Phosphatidylinositol 3- and 4-kinase, catalytic)|interpro:IPR003151(PIK-related kinase, FAT)|interpro:IPR003152(PIK-related kinase, FATC)|interpro:IPR011009(Protein kinase-like)|interpro:IPR014009(PIK-related kinase)|interpro:IPR016024(Armadillo-type fold)|interpro:IPR033317|interpro:IPR036940|rcsb pdb:5OJS|rcsb pdb:6IG9|rcsb pdb:6T9I|rcsb pdb:6T9J	-	crc64:2ED1B8E35D509578	crc64:AE3588676F5D5777	figure legend:f5e|comment:"we tested whether the lack of the Taf9 CRD  impaired the interaction of Gcn4 with the SAGA complex. GST  pulldown assays (9, 23) using GST-Gcn4 showed interaction of  Spt7, Gcn5, Tra1, Taf12, and Taf9 from the TAF9-HA3 extract in a  manner dependent on the WT Gcn4 activation domain (Fig. 5E).  As expected from our previous study (9), the SWI/SNF subunit  Snf6, used as a control here, was specifically pulled down. Whereas  no interaction with Taf1 was seen, interaction with TBP did not  show specificity for the Gcn4 activation domain, as observed previously  (9, 23). The pulldown assays conducted from the tCRD2-  HA3 extract also showed the pulldown of the same SAGA subunits,  albeit with reduced recovery of the Gcn5 subunit (Fig. 5E).  Importantly, the interaction of Gcn4 with Tra1 and Taf12 was not  impaired in the tCRD2-HA3 extract (Fig. 5E). Together, these results  indicate that the interactions of the Gcn4 activation domain  with the mutant SAGA complex are largely intact in vitro."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2015/05/19	2015/05/21	rogid:MFj9JcYccWKTayvnuaN95djsHd0559292	rogid:B/rkvJoIvK1Xi2SoNtFnFOdcp2c559292	rigid:h7fKB+ckA1LiSRUrDgYoc5WOKhE	false	glutathione s tranferase tag:?-?	-	-	-	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)
uniprotkb:P03069	uniprotkb:Q05027	intact:EBI-7450|uniprotkb:P03068|uniprotkb:Q70D88|uniprotkb:Q70D91|uniprotkb:Q70D96|uniprotkb:Q70D99|uniprotkb:Q70DA0|uniprotkb:Q96UT3|uniprotkb:D3DLN9|ensemblfungi:YEL009C	intact:EBI-27500|uniprotkb:D6W062|ensemblfungi:YMR236W	psi-mi:gcn4_yeast(display_long)|uniprotkb:ARG9(gene name synonym)|uniprotkb:GCN4(gene name)|psi-mi:GCN4(display_short)|uniprotkb:AAS3(gene name synonym)|uniprotkb:Amino acid biosynthesis regulatory protein(gene name synonym)|uniprotkb:AAS101(gene name synonym)|uniprotkb:General control protein GCN4(gene name synonym)|uniprotkb:YEL009C(locus name)	psi-mi:taf9_yeast(display_long)|uniprotkb:TAF9(gene name)|psi-mi:TAF9(display_short)|uniprotkb:TAF17(gene name synonym)|uniprotkb:YMR236W(locus name)|uniprotkb:YM9959.18(orf name)|uniprotkb:TBP-associated factor 9(gene name synonym)|uniprotkb:TBP-associated factor 17 kDa(gene name synonym)|uniprotkb:TAFII-17(gene name synonym)|uniprotkb:TAFII20(gene name synonym)	psi-mi:"MI:0096"(pull down)	Saint et al. (2014)	pubmed:24550006|imex:IM-24168	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-10823079|imex:IM-24168-4	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	sgd:S000000735|refseq:NP_010907.3|mint:P03069|dip:DIP-591N|ensemblfungi:YEL009C(gene)|ensemblfungi:YEL009C_mRNA(transcript)|go:"GO:0000122"(negative regulation of transcription by RNA polymerase II)|go:"GO:0000978"(RNA polymerase II cis-regulatory region sequence-specific DNA binding)|go:"GO:0000981"(DNA-binding transcription factor activity, RNA polymerase II-specific)|go:"GO:0001080"(nitrogen catabolite activation of transcription from RNA polymerase II promoter)|go:"GO:0001228"(DNA-binding transcription activator activity, RNA polymerase II-specific)|go:"GO:0003682"(chromatin binding)|go:"GO:0003700"(DNA-binding transcription factor activity)|go:"GO:0005634"(nucleus)|go:"GO:0005667"(transcription regulator complex)|go:"GO:0006357"(regulation of transcription by RNA polymerase II)|go:"GO:0006914"(autophagy)|go:"GO:0008652"(cellular amino acid biosynthetic process)|go:"GO:0010508"(positive regulation of autophagy)|go:"GO:0010691"(negative regulation of ribosomal protein gene transcription from RNA polymerase II promoter in response to nutrient levels)|go:"GO:0035556"(intracellular signal transduction)|go:"GO:0036278"(positive regulation of transcription from RNA polymerase II promoter in response to nitrogen starvation)|go:"GO:0042802"(identical protein binding)|go:"GO:0043565"(sequence-specific DNA binding)|go:"GO:0043621"(protein self-association)|go:"GO:0045899"(positive regulation of RNA polymerase II transcription preinitiation complex assembly)|go:"GO:0045944"(positive regulation of transcription by RNA polymerase II)|go:"GO:0060261"(positive regulation of transcription initiation from RNA polymerase II promoter)|go:"GO:0061412"(positive regulation of transcription from RNA polymerase II promoter in response to amino acid starvation)|go:"GO:0061629"(RNA polymerase II-specific DNA-binding transcription factor binding)|go:"GO:0090575"(RNA polymerase II transcription regulator complex)|go:"GO:1903833"(positive regulation of cellular response to amino acid starvation)|go:"GO:1990139"(protein localization to nuclear periphery)|go:"GO:1990928"(response to amino acid starvation)|interpro:IPR004827("Basic-leucine zipper (bZIP) transcription factor")|rcsb pdb:1CE9|rcsb pdb:1DGC|rcsb pdb:1ENV|rcsb pdb:1FAV|rcsb pdb:1FMH|rcsb pdb:1GCL|rcsb pdb:1GCM|rcsb pdb:1GK6|rcsb pdb:1GZL|rcsb pdb:1IHQ|rcsb pdb:1IJ0|rcsb pdb:1IJ1|rcsb pdb:1IJ2|rcsb pdb:1IJ3|rcsb pdb:1KQL|rcsb pdb:1LD4|rcsb pdb:1LLM|rcsb pdb:1NKN|rcsb pdb:1PIQ|rcsb pdb:1RB4|rcsb pdb:1RB5|rcsb pdb:1RB6|rcsb pdb:1SWI|rcsb pdb:1TMZ|rcsb pdb:1UNT|rcsb pdb:1UNU|rcsb pdb:1UNV|rcsb pdb:1UNW|rcsb pdb:1UNX|rcsb pdb:1UNY|rcsb pdb:1UNZ|rcsb pdb:1UO0|rcsb pdb:1UO1|rcsb pdb:1UO2|rcsb pdb:1UO3|rcsb pdb:1UO4|rcsb pdb:1UO5|rcsb pdb:1W5G|rcsb pdb:1W5H|rcsb pdb:1W5I|rcsb pdb:1W5J|rcsb pdb:1W5K|rcsb pdb:1W5L|rcsb pdb:1YSA|rcsb pdb:1ZII|rcsb pdb:1ZIJ|rcsb pdb:1ZIK|rcsb pdb:1ZIL|rcsb pdb:1ZIM|rcsb pdb:1ZTA|rcsb pdb:2AHP|rcsb pdb:2B1F|rcsb pdb:2B22|rcsb pdb:2BNI|rcsb pdb:2CCE|rcsb pdb:2CCF|rcsb pdb:2CCN|rcsb pdb:2D3E|rcsb pdb:2DGC|rcsb pdb:2EFR|rcsb pdb:2EFS|rcsb pdb:2G9J|rcsb pdb:2HY6|rcsb pdb:2IPZ|rcsb pdb:2K8X|rcsb pdb:2LPB|rcsb pdb:2N9B|rcsb pdb:2NRN|rcsb pdb:2O7H|rcsb pdb:2OVN|rcsb pdb:2VKY|rcsb pdb:2VNL|rcsb pdb:2WG5|rcsb pdb:2WG6|rcsb pdb:2WPY|rcsb pdb:2WPZ|rcsb pdb:2WQ0|rcsb pdb:2WQ1|rcsb pdb:2WQ2|rcsb pdb:2WQ3|rcsb pdb:2YNY|rcsb pdb:2YNZ|rcsb pdb:2YO0|rcsb pdb:2YO1|rcsb pdb:2YO2|rcsb pdb:2YO3|rcsb pdb:2Z5H|rcsb pdb:2Z5I|rcsb pdb:2ZTA|rcsb pdb:3AZD|rcsb pdb:3BAS|rcsb pdb:3BAT|rcsb pdb:3CK4|rcsb pdb:3CRP|rcsb pdb:3G9R|rcsb pdb:3GJP|rcsb pdb:3I1G|rcsb pdb:3I5C|rcsb pdb:3K7Z|rcsb pdb:3M48|rcsb pdb:3P8M|rcsb pdb:3ZMF|rcsb pdb:4C46|rcsb pdb:4DMD|rcsb pdb:4G2K|rcsb pdb:4HU5|rcsb pdb:4HU6|rcsb pdb:4NIZ|rcsb pdb:4NJ0|rcsb pdb:4NJ1|rcsb pdb:4NJ2|rcsb pdb:4OWI|rcsb pdb:4TL1|rcsb pdb:5APP|rcsb pdb:5APQ|rcsb pdb:5APS|rcsb pdb:5APT|rcsb pdb:5APU|rcsb pdb:5APV|rcsb pdb:5APW|rcsb pdb:5APX|rcsb pdb:5APY|rcsb pdb:5APZ|rcsb pdb:5IEW|rcsb pdb:5IIR|rcsb pdb:5IIV|rcsb pdb:5KHT|rcsb pdb:6E52|rcsb pdb:6E95|rcsb pdb:6H9M|rcsb pdb:6HN4|rcsb pdb:6HN5|rcsb pdb:6O2E|rcsb pdb:6O2F|rcsb pdb:6PSA|rcsb pdb:6UT2|rcsb pdb:6VWG|rcsb pdb:6VWH|rcsb pdb:6VWI|rcsb pdb:6VWJ|reactome:R-SCE-2559580|reactome:R-SCE-2871796|reactome:R-SCE-450341	mint:Q05027|refseq:NP_013963.1|sgd:S000004849|dip:DIP-925N|ensemblfungi:YMR236W(gene)|ensemblfungi:YMR236W_mRNA(transcript)|go:"GO:0000124"(SAGA complex)|go:"GO:0003682"(chromatin binding)|go:"GO:0003713"(transcription coactivator activity)|go:"GO:0005669"(transcription factor TFIID complex)|go:"GO:0006325"(chromatin organization)|go:"GO:0006366"(transcription by RNA polymerase II)|go:"GO:0016573"(histone acetylation)|go:"GO:0042802"(identical protein binding)|go:"GO:0043966"(histone H3 acetylation)|go:"GO:0045944"(positive regulation of transcription by RNA polymerase II)|go:"GO:0046695"("SLIK (SAGA-like) complex")|go:"GO:0046982"(protein heterodimerization activity)|go:"GO:0051123"(RNA polymerase II preinitiation complex assembly)|go:"GO:0060090"(molecular adaptor activity)|interpro:IPR003162(Transcription factor TAFII-31)|interpro:IPR009072(Histone-fold)|rcsb pdb:6T9I|rcsb pdb:6T9K|reactome:R-SCE-5689880|reactome:R-SCE-674695|reactome:R-SCE-6807505|reactome:R-SCE-73776|reactome:R-SCE-73779|reactome:R-SCE-75953|reactome:R-SCE-76042	-	crc64:2ED1B8E35D509578	crc64:D1C97EDDCA468EC5	figure legend:f5e|comment:"we tested whether the lack of the Taf9 CRD  impaired the interaction of Gcn4 with the SAGA complex. GST  pulldown assays (9, 23) using GST-Gcn4 showed interaction of  Spt7, Gcn5, Tra1, Taf12, and Taf9 from the TAF9-HA3 extract in a  manner dependent on the WT Gcn4 activation domain (Fig. 5E).  As expected from our previous study (9), the SWI/SNF subunit  Snf6, used as a control here, was specifically pulled down. Whereas  no interaction with Taf1 was seen, interaction with TBP did not  show specificity for the Gcn4 activation domain, as observed previously  (9, 23). The pulldown assays conducted from the tCRD2-  HA3 extract also showed the pulldown of the same SAGA subunits,  albeit with reduced recovery of the Gcn5 subunit (Fig. 5E).  Importantly, the interaction of Gcn4 with Tra1 and Taf12 was not  impaired in the tCRD2-HA3 extract (Fig. 5E). Together, these results  indicate that the interactions of the Gcn4 activation domain  with the mutant SAGA complex are largely intact in vitro."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2015/05/19	2015/05/21	rogid:MFj9JcYccWKTayvnuaN95djsHd0559292	rogid:3+S7ozz3r1gCMvBUUfkGpeTCy/k559292	rigid:h7fKB+ckA1LiSRUrDgYoc5WOKhE	false	glutathione s tranferase tag:?-?	binding-associated region:1-131	-	-	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)
uniprotkb:P03069	uniprotkb:Q03761	intact:EBI-7450|uniprotkb:P03068|uniprotkb:Q70D88|uniprotkb:Q70D91|uniprotkb:Q70D96|uniprotkb:Q70D99|uniprotkb:Q70DA0|uniprotkb:Q96UT3|uniprotkb:D3DLN9|ensemblfungi:YEL009C	intact:EBI-35097|uniprotkb:D6VSC8|ensemblfungi:YDR145W	psi-mi:gcn4_yeast(display_long)|uniprotkb:ARG9(gene name synonym)|uniprotkb:GCN4(gene name)|psi-mi:GCN4(display_short)|uniprotkb:AAS3(gene name synonym)|uniprotkb:Amino acid biosynthesis regulatory protein(gene name synonym)|uniprotkb:AAS101(gene name synonym)|uniprotkb:General control protein GCN4(gene name synonym)|uniprotkb:YEL009C(locus name)	psi-mi:taf12_yeast(display_long)|uniprotkb:TAF12(gene name)|psi-mi:TAF12(display_short)|uniprotkb:TAF61(gene name synonym)|uniprotkb:TAF68(gene name synonym)|uniprotkb:YDR145W(locus name)|uniprotkb:YD8358.02(orf name)|uniprotkb:TBP-associated factor 12(gene name synonym)|uniprotkb:TBP-associated factor 61 kDa(gene name synonym)|uniprotkb:TAFII-61(gene name synonym)|uniprotkb:TAFII-68(gene name synonym)	psi-mi:"MI:0096"(pull down)	Saint et al. (2014)	pubmed:24550006|imex:IM-24168	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-10823079|imex:IM-24168-4	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	sgd:S000000735|refseq:NP_010907.3|mint:P03069|dip:DIP-591N|ensemblfungi:YEL009C(gene)|ensemblfungi:YEL009C_mRNA(transcript)|go:"GO:0000122"(negative regulation of transcription by RNA polymerase II)|go:"GO:0000978"(RNA polymerase II cis-regulatory region sequence-specific DNA binding)|go:"GO:0000981"(DNA-binding transcription factor activity, RNA polymerase II-specific)|go:"GO:0001080"(nitrogen catabolite activation of transcription from RNA polymerase II promoter)|go:"GO:0001228"(DNA-binding transcription activator activity, RNA polymerase II-specific)|go:"GO:0003682"(chromatin binding)|go:"GO:0003700"(DNA-binding transcription factor activity)|go:"GO:0005634"(nucleus)|go:"GO:0005667"(transcription regulator complex)|go:"GO:0006357"(regulation of transcription by RNA polymerase II)|go:"GO:0006914"(autophagy)|go:"GO:0008652"(cellular amino acid biosynthetic process)|go:"GO:0010508"(positive regulation of autophagy)|go:"GO:0010691"(negative regulation of ribosomal protein gene transcription from RNA polymerase II promoter in response to nutrient levels)|go:"GO:0035556"(intracellular signal transduction)|go:"GO:0036278"(positive regulation of transcription from RNA polymerase II promoter in response to nitrogen starvation)|go:"GO:0042802"(identical protein binding)|go:"GO:0043565"(sequence-specific DNA binding)|go:"GO:0043621"(protein self-association)|go:"GO:0045899"(positive regulation of RNA polymerase II transcription preinitiation complex assembly)|go:"GO:0045944"(positive regulation of transcription by RNA polymerase II)|go:"GO:0060261"(positive regulation of transcription initiation from RNA polymerase II promoter)|go:"GO:0061412"(positive regulation of transcription from RNA polymerase II promoter in response to amino acid starvation)|go:"GO:0061629"(RNA polymerase II-specific DNA-binding transcription factor binding)|go:"GO:0090575"(RNA polymerase II transcription regulator complex)|go:"GO:1903833"(positive regulation of cellular response to amino acid starvation)|go:"GO:1990139"(protein localization to nuclear periphery)|go:"GO:1990928"(response to amino acid starvation)|interpro:IPR004827("Basic-leucine zipper (bZIP) transcription factor")|rcsb pdb:1CE9|rcsb pdb:1DGC|rcsb pdb:1ENV|rcsb pdb:1FAV|rcsb pdb:1FMH|rcsb pdb:1GCL|rcsb pdb:1GCM|rcsb pdb:1GK6|rcsb pdb:1GZL|rcsb pdb:1IHQ|rcsb pdb:1IJ0|rcsb pdb:1IJ1|rcsb pdb:1IJ2|rcsb pdb:1IJ3|rcsb pdb:1KQL|rcsb pdb:1LD4|rcsb pdb:1LLM|rcsb pdb:1NKN|rcsb pdb:1PIQ|rcsb pdb:1RB4|rcsb pdb:1RB5|rcsb pdb:1RB6|rcsb pdb:1SWI|rcsb pdb:1TMZ|rcsb pdb:1UNT|rcsb pdb:1UNU|rcsb pdb:1UNV|rcsb pdb:1UNW|rcsb pdb:1UNX|rcsb pdb:1UNY|rcsb pdb:1UNZ|rcsb pdb:1UO0|rcsb pdb:1UO1|rcsb pdb:1UO2|rcsb pdb:1UO3|rcsb pdb:1UO4|rcsb pdb:1UO5|rcsb pdb:1W5G|rcsb pdb:1W5H|rcsb pdb:1W5I|rcsb pdb:1W5J|rcsb pdb:1W5K|rcsb pdb:1W5L|rcsb pdb:1YSA|rcsb pdb:1ZII|rcsb pdb:1ZIJ|rcsb pdb:1ZIK|rcsb pdb:1ZIL|rcsb pdb:1ZIM|rcsb pdb:1ZTA|rcsb pdb:2AHP|rcsb pdb:2B1F|rcsb pdb:2B22|rcsb pdb:2BNI|rcsb pdb:2CCE|rcsb pdb:2CCF|rcsb pdb:2CCN|rcsb pdb:2D3E|rcsb pdb:2DGC|rcsb pdb:2EFR|rcsb pdb:2EFS|rcsb pdb:2G9J|rcsb pdb:2HY6|rcsb pdb:2IPZ|rcsb pdb:2K8X|rcsb pdb:2LPB|rcsb pdb:2N9B|rcsb pdb:2NRN|rcsb pdb:2O7H|rcsb pdb:2OVN|rcsb pdb:2VKY|rcsb pdb:2VNL|rcsb pdb:2WG5|rcsb pdb:2WG6|rcsb pdb:2WPY|rcsb pdb:2WPZ|rcsb pdb:2WQ0|rcsb pdb:2WQ1|rcsb pdb:2WQ2|rcsb pdb:2WQ3|rcsb pdb:2YNY|rcsb pdb:2YNZ|rcsb pdb:2YO0|rcsb pdb:2YO1|rcsb pdb:2YO2|rcsb pdb:2YO3|rcsb pdb:2Z5H|rcsb pdb:2Z5I|rcsb pdb:2ZTA|rcsb pdb:3AZD|rcsb pdb:3BAS|rcsb pdb:3BAT|rcsb pdb:3CK4|rcsb pdb:3CRP|rcsb pdb:3G9R|rcsb pdb:3GJP|rcsb pdb:3I1G|rcsb pdb:3I5C|rcsb pdb:3K7Z|rcsb pdb:3M48|rcsb pdb:3P8M|rcsb pdb:3ZMF|rcsb pdb:4C46|rcsb pdb:4DMD|rcsb pdb:4G2K|rcsb pdb:4HU5|rcsb pdb:4HU6|rcsb pdb:4NIZ|rcsb pdb:4NJ0|rcsb pdb:4NJ1|rcsb pdb:4NJ2|rcsb pdb:4OWI|rcsb pdb:4TL1|rcsb pdb:5APP|rcsb pdb:5APQ|rcsb pdb:5APS|rcsb pdb:5APT|rcsb pdb:5APU|rcsb pdb:5APV|rcsb pdb:5APW|rcsb pdb:5APX|rcsb pdb:5APY|rcsb pdb:5APZ|rcsb pdb:5IEW|rcsb pdb:5IIR|rcsb pdb:5IIV|rcsb pdb:5KHT|rcsb pdb:6E52|rcsb pdb:6E95|rcsb pdb:6H9M|rcsb pdb:6HN4|rcsb pdb:6HN5|rcsb pdb:6O2E|rcsb pdb:6O2F|rcsb pdb:6PSA|rcsb pdb:6UT2|rcsb pdb:6VWG|rcsb pdb:6VWH|rcsb pdb:6VWI|rcsb pdb:6VWJ|reactome:R-SCE-2559580|reactome:R-SCE-2871796|reactome:R-SCE-450341	refseq:NP_010429.1|sgd:S000002552|mint:Q03761|dip:DIP-1302N|ensemblfungi:YDR145W(gene)|ensemblfungi:YDR145W_mRNA(transcript)|go:"GO:0000124"(SAGA complex)|go:"GO:0003677"(DNA binding)|go:"GO:0003682"(chromatin binding)|go:"GO:0005669"(transcription factor TFIID complex)|go:"GO:0006325"(chromatin organization)|go:"GO:0006366"(transcription by RNA polymerase II)|go:"GO:0016573"(histone acetylation)|go:"GO:0017025"(TBP-class protein binding)|go:"GO:0042802"(identical protein binding)|go:"GO:0046695"("SLIK (SAGA-like) complex")|go:"GO:0046982"(protein heterodimerization activity)|go:"GO:0051123"(RNA polymerase II preinitiation complex assembly)|go:"GO:0060090"(molecular adaptor activity)|go:"GO:0061629"(RNA polymerase II-specific DNA-binding transcription factor binding)|interpro:IPR003228(Transcription initiation factor TFIID)|interpro:IPR009072(Histone-fold)|interpro:IPR037794|rcsb pdb:6T9I|rcsb pdb:6T9J|rcsb pdb:6T9K|reactome:R-SCE-674695|reactome:R-SCE-73776|reactome:R-SCE-73779|reactome:R-SCE-75953|reactome:R-SCE-76042	-	crc64:2ED1B8E35D509578	crc64:A8FC84E46F8370D3	figure legend:f5e|comment:"we tested whether the lack of the Taf9 CRD  impaired the interaction of Gcn4 with the SAGA complex. GST  pulldown assays (9, 23) using GST-Gcn4 showed interaction of  Spt7, Gcn5, Tra1, Taf12, and Taf9 from the TAF9-HA3 extract in a  manner dependent on the WT Gcn4 activation domain (Fig. 5E).  As expected from our previous study (9), the SWI/SNF subunit  Snf6, used as a control here, was specifically pulled down. Whereas  no interaction with Taf1 was seen, interaction with TBP did not  show specificity for the Gcn4 activation domain, as observed previously  (9, 23). The pulldown assays conducted from the tCRD2-  HA3 extract also showed the pulldown of the same SAGA subunits,  albeit with reduced recovery of the Gcn5 subunit (Fig. 5E).  Importantly, the interaction of Gcn4 with Tra1 and Taf12 was not  impaired in the tCRD2-HA3 extract (Fig. 5E). Together, these results  indicate that the interactions of the Gcn4 activation domain  with the mutant SAGA complex are largely intact in vitro."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2015/05/19	2015/05/21	rogid:MFj9JcYccWKTayvnuaN95djsHd0559292	rogid:DCTuNehYD4fhp7PRgtHZyNIDbH4559292	rigid:h7fKB+ckA1LiSRUrDgYoc5WOKhE	false	glutathione s tranferase tag:?-?	-	-	-	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)
uniprotkb:P03069	uniprotkb:Q03330	intact:EBI-7450|uniprotkb:P03068|uniprotkb:Q70D88|uniprotkb:Q70D91|uniprotkb:Q70D96|uniprotkb:Q70D99|uniprotkb:Q70DA0|uniprotkb:Q96UT3|uniprotkb:D3DLN9|ensemblfungi:YEL009C	intact:EBI-7458|uniprotkb:Q6B165|uniprotkb:D6VV32|ensemblfungi:YGR252W	psi-mi:gcn4_yeast(display_long)|uniprotkb:ARG9(gene name synonym)|uniprotkb:GCN4(gene name)|psi-mi:GCN4(display_short)|uniprotkb:AAS3(gene name synonym)|uniprotkb:Amino acid biosynthesis regulatory protein(gene name synonym)|uniprotkb:AAS101(gene name synonym)|uniprotkb:General control protein GCN4(gene name synonym)|uniprotkb:YEL009C(locus name)	psi-mi:gcn5_yeast(display_long)|uniprotkb:YGR252W(locus name)|uniprotkb:GCN5(gene name)|psi-mi:GCN5(display_short)|uniprotkb:ADA4(gene name synonym)|uniprotkb:SWI9(gene name synonym)	psi-mi:"MI:0096"(pull down)	Saint et al. (2014)	pubmed:24550006|imex:IM-24168	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-10823079|imex:IM-24168-4	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	sgd:S000000735|refseq:NP_010907.3|mint:P03069|dip:DIP-591N|ensemblfungi:YEL009C(gene)|ensemblfungi:YEL009C_mRNA(transcript)|go:"GO:0000122"(negative regulation of transcription by RNA polymerase II)|go:"GO:0000978"(RNA polymerase II cis-regulatory region sequence-specific DNA binding)|go:"GO:0000981"(DNA-binding transcription factor activity, RNA polymerase II-specific)|go:"GO:0001080"(nitrogen catabolite activation of transcription from RNA polymerase II promoter)|go:"GO:0001228"(DNA-binding transcription activator activity, RNA polymerase II-specific)|go:"GO:0003682"(chromatin binding)|go:"GO:0003700"(DNA-binding transcription factor activity)|go:"GO:0005634"(nucleus)|go:"GO:0005667"(transcription regulator complex)|go:"GO:0006357"(regulation of transcription by RNA polymerase II)|go:"GO:0006914"(autophagy)|go:"GO:0008652"(cellular amino acid biosynthetic process)|go:"GO:0010508"(positive regulation of autophagy)|go:"GO:0010691"(negative regulation of ribosomal protein gene transcription from RNA polymerase II promoter in response to nutrient levels)|go:"GO:0035556"(intracellular signal transduction)|go:"GO:0036278"(positive regulation of transcription from RNA polymerase II promoter in response to nitrogen starvation)|go:"GO:0042802"(identical protein binding)|go:"GO:0043565"(sequence-specific DNA binding)|go:"GO:0043621"(protein self-association)|go:"GO:0045899"(positive regulation of RNA polymerase II transcription preinitiation complex assembly)|go:"GO:0045944"(positive regulation of transcription by RNA polymerase II)|go:"GO:0060261"(positive regulation of transcription initiation from RNA polymerase II promoter)|go:"GO:0061412"(positive regulation of transcription from RNA polymerase II promoter in response to amino acid starvation)|go:"GO:0061629"(RNA polymerase II-specific DNA-binding transcription factor binding)|go:"GO:0090575"(RNA polymerase II transcription regulator complex)|go:"GO:1903833"(positive regulation of cellular response to amino acid starvation)|go:"GO:1990139"(protein localization to nuclear periphery)|go:"GO:1990928"(response to amino acid starvation)|interpro:IPR004827("Basic-leucine zipper (bZIP) transcription factor")|rcsb pdb:1CE9|rcsb pdb:1DGC|rcsb pdb:1ENV|rcsb pdb:1FAV|rcsb pdb:1FMH|rcsb pdb:1GCL|rcsb pdb:1GCM|rcsb pdb:1GK6|rcsb pdb:1GZL|rcsb pdb:1IHQ|rcsb pdb:1IJ0|rcsb pdb:1IJ1|rcsb pdb:1IJ2|rcsb pdb:1IJ3|rcsb pdb:1KQL|rcsb pdb:1LD4|rcsb pdb:1LLM|rcsb pdb:1NKN|rcsb pdb:1PIQ|rcsb pdb:1RB4|rcsb pdb:1RB5|rcsb pdb:1RB6|rcsb pdb:1SWI|rcsb pdb:1TMZ|rcsb pdb:1UNT|rcsb pdb:1UNU|rcsb pdb:1UNV|rcsb pdb:1UNW|rcsb pdb:1UNX|rcsb pdb:1UNY|rcsb pdb:1UNZ|rcsb pdb:1UO0|rcsb pdb:1UO1|rcsb pdb:1UO2|rcsb pdb:1UO3|rcsb pdb:1UO4|rcsb pdb:1UO5|rcsb pdb:1W5G|rcsb pdb:1W5H|rcsb pdb:1W5I|rcsb pdb:1W5J|rcsb pdb:1W5K|rcsb pdb:1W5L|rcsb pdb:1YSA|rcsb pdb:1ZII|rcsb pdb:1ZIJ|rcsb pdb:1ZIK|rcsb pdb:1ZIL|rcsb pdb:1ZIM|rcsb pdb:1ZTA|rcsb pdb:2AHP|rcsb pdb:2B1F|rcsb pdb:2B22|rcsb pdb:2BNI|rcsb pdb:2CCE|rcsb pdb:2CCF|rcsb pdb:2CCN|rcsb pdb:2D3E|rcsb pdb:2DGC|rcsb pdb:2EFR|rcsb pdb:2EFS|rcsb pdb:2G9J|rcsb pdb:2HY6|rcsb pdb:2IPZ|rcsb pdb:2K8X|rcsb pdb:2LPB|rcsb pdb:2N9B|rcsb pdb:2NRN|rcsb pdb:2O7H|rcsb pdb:2OVN|rcsb pdb:2VKY|rcsb pdb:2VNL|rcsb pdb:2WG5|rcsb pdb:2WG6|rcsb pdb:2WPY|rcsb pdb:2WPZ|rcsb pdb:2WQ0|rcsb pdb:2WQ1|rcsb pdb:2WQ2|rcsb pdb:2WQ3|rcsb pdb:2YNY|rcsb pdb:2YNZ|rcsb pdb:2YO0|rcsb pdb:2YO1|rcsb pdb:2YO2|rcsb pdb:2YO3|rcsb pdb:2Z5H|rcsb pdb:2Z5I|rcsb pdb:2ZTA|rcsb pdb:3AZD|rcsb pdb:3BAS|rcsb pdb:3BAT|rcsb pdb:3CK4|rcsb pdb:3CRP|rcsb pdb:3G9R|rcsb pdb:3GJP|rcsb pdb:3I1G|rcsb pdb:3I5C|rcsb pdb:3K7Z|rcsb pdb:3M48|rcsb pdb:3P8M|rcsb pdb:3ZMF|rcsb pdb:4C46|rcsb pdb:4DMD|rcsb pdb:4G2K|rcsb pdb:4HU5|rcsb pdb:4HU6|rcsb pdb:4NIZ|rcsb pdb:4NJ0|rcsb pdb:4NJ1|rcsb pdb:4NJ2|rcsb pdb:4OWI|rcsb pdb:4TL1|rcsb pdb:5APP|rcsb pdb:5APQ|rcsb pdb:5APS|rcsb pdb:5APT|rcsb pdb:5APU|rcsb pdb:5APV|rcsb pdb:5APW|rcsb pdb:5APX|rcsb pdb:5APY|rcsb pdb:5APZ|rcsb pdb:5IEW|rcsb pdb:5IIR|rcsb pdb:5IIV|rcsb pdb:5KHT|rcsb pdb:6E52|rcsb pdb:6E95|rcsb pdb:6H9M|rcsb pdb:6HN4|rcsb pdb:6HN5|rcsb pdb:6O2E|rcsb pdb:6O2F|rcsb pdb:6PSA|rcsb pdb:6UT2|rcsb pdb:6VWG|rcsb pdb:6VWH|rcsb pdb:6VWI|rcsb pdb:6VWJ|reactome:R-SCE-2559580|reactome:R-SCE-2871796|reactome:R-SCE-450341	refseq:NP_011768.1|sgd:S000003484|mint:Q03330|dip:DIP-710N|ensemblfungi:YGR252W(gene)|ensemblfungi:YGR252W_mRNA(transcript)|go:"GO:0000124"(SAGA complex)|go:"GO:0000775"(chromosome, centromeric region)|go:"GO:0003712"(transcription coregulator activity)|go:"GO:0004402"(histone acetyltransferase activity)|go:"GO:0005634"(nucleus)|go:"GO:0005671"(obsolete Ada2/Gcn5/Ada3 transcription activator complex)|go:"GO:0005829"(cytosol)|go:"GO:0006325"(chromatin organization)|go:"GO:0006338"(chromatin remodeling)|go:"GO:0010484"(H3 histone acetyltransferase activity)|go:"GO:0016573"(histone acetylation)|go:"GO:0032968"(positive regulation of transcription elongation from RNA polymerase II promoter)|go:"GO:0046695"("SLIK (SAGA-like) complex")|go:"GO:0070577"(lysine-acetylated histone binding)|go:"GO:0140068"(histone crotonyltransferase activity)|interpro:IPR000182(GCN5-related N-acetyltransferase)|interpro:IPR001487(Bromodomain)|interpro:IPR016181(Acyl-CoA N-acyltransferase)|interpro:IPR018359|interpro:IPR036427|interpro:IPR037800|rcsb pdb:1E6I|rcsb pdb:1YGH|rcsb pdb:6CW2|rcsb pdb:6CW3|reactome:R-SCE-5689880|reactome:R-SCE-9018519	-	crc64:2ED1B8E35D509578	crc64:3200730DDC7EF70D	figure legend:f5e|comment:"we tested whether the lack of the Taf9 CRD  impaired the interaction of Gcn4 with the SAGA complex. GST  pulldown assays (9, 23) using GST-Gcn4 showed interaction of  Spt7, Gcn5, Tra1, Taf12, and Taf9 from the TAF9-HA3 extract in a  manner dependent on the WT Gcn4 activation domain (Fig. 5E).  As expected from our previous study (9), the SWI/SNF subunit  Snf6, used as a control here, was specifically pulled down. Whereas  no interaction with Taf1 was seen, interaction with TBP did not  show specificity for the Gcn4 activation domain, as observed previously  (9, 23). The pulldown assays conducted from the tCRD2-  HA3 extract also showed the pulldown of the same SAGA subunits,  albeit with reduced recovery of the Gcn5 subunit (Fig. 5E).  Importantly, the interaction of Gcn4 with Tra1 and Taf12 was not  impaired in the tCRD2-HA3 extract (Fig. 5E). Together, these results  indicate that the interactions of the Gcn4 activation domain  with the mutant SAGA complex are largely intact in vitro."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2015/05/19	2015/05/21	rogid:MFj9JcYccWKTayvnuaN95djsHd0559292	rogid:Y8SHidiW4dJo0oxEDksur8MC/L0559292	rigid:h7fKB+ckA1LiSRUrDgYoc5WOKhE	false	glutathione s tranferase tag:?-?	-	-	-	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)
uniprotkb:P03069	uniprotkb:P35177	intact:EBI-7450|uniprotkb:P03068|uniprotkb:Q70D88|uniprotkb:Q70D91|uniprotkb:Q70D96|uniprotkb:Q70D99|uniprotkb:Q70DA0|uniprotkb:Q96UT3|uniprotkb:D3DLN9|ensemblfungi:YEL009C	intact:EBI-17958|uniprotkb:D6VQ80|ensemblfungi:YBR081C	psi-mi:gcn4_yeast(display_long)|uniprotkb:ARG9(gene name synonym)|uniprotkb:GCN4(gene name)|psi-mi:GCN4(display_short)|uniprotkb:AAS3(gene name synonym)|uniprotkb:Amino acid biosynthesis regulatory protein(gene name synonym)|uniprotkb:AAS101(gene name synonym)|uniprotkb:General control protein GCN4(gene name synonym)|uniprotkb:YEL009C(locus name)	psi-mi:spt7_yeast(display_long)|uniprotkb:YBR0739(orf name)|uniprotkb:YBR081C(locus name)|uniprotkb:SPT7(gene name)|psi-mi:SPT7(display_short)	psi-mi:"MI:0096"(pull down)	Saint et al. (2014)	pubmed:24550006|imex:IM-24168	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-10823079|imex:IM-24168-4	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	sgd:S000000735|refseq:NP_010907.3|mint:P03069|dip:DIP-591N|ensemblfungi:YEL009C(gene)|ensemblfungi:YEL009C_mRNA(transcript)|go:"GO:0000122"(negative regulation of transcription by RNA polymerase II)|go:"GO:0000978"(RNA polymerase II cis-regulatory region sequence-specific DNA binding)|go:"GO:0000981"(DNA-binding transcription factor activity, RNA polymerase II-specific)|go:"GO:0001080"(nitrogen catabolite activation of transcription from RNA polymerase II promoter)|go:"GO:0001228"(DNA-binding transcription activator activity, RNA polymerase II-specific)|go:"GO:0003682"(chromatin binding)|go:"GO:0003700"(DNA-binding transcription factor activity)|go:"GO:0005634"(nucleus)|go:"GO:0005667"(transcription regulator complex)|go:"GO:0006357"(regulation of transcription by RNA polymerase II)|go:"GO:0006914"(autophagy)|go:"GO:0008652"(cellular amino acid biosynthetic process)|go:"GO:0010508"(positive regulation of autophagy)|go:"GO:0010691"(negative regulation of ribosomal protein gene transcription from RNA polymerase II promoter in response to nutrient levels)|go:"GO:0035556"(intracellular signal transduction)|go:"GO:0036278"(positive regulation of transcription from RNA polymerase II promoter in response to nitrogen starvation)|go:"GO:0042802"(identical protein binding)|go:"GO:0043565"(sequence-specific DNA binding)|go:"GO:0043621"(protein self-association)|go:"GO:0045899"(positive regulation of RNA polymerase II transcription preinitiation complex assembly)|go:"GO:0045944"(positive regulation of transcription by RNA polymerase II)|go:"GO:0060261"(positive regulation of transcription initiation from RNA polymerase II promoter)|go:"GO:0061412"(positive regulation of transcription from RNA polymerase II promoter in response to amino acid starvation)|go:"GO:0061629"(RNA polymerase II-specific DNA-binding transcription factor binding)|go:"GO:0090575"(RNA polymerase II transcription regulator complex)|go:"GO:1903833"(positive regulation of cellular response to amino acid starvation)|go:"GO:1990139"(protein localization to nuclear periphery)|go:"GO:1990928"(response to amino acid starvation)|interpro:IPR004827("Basic-leucine zipper (bZIP) transcription factor")|rcsb pdb:1CE9|rcsb pdb:1DGC|rcsb pdb:1ENV|rcsb pdb:1FAV|rcsb pdb:1FMH|rcsb pdb:1GCL|rcsb pdb:1GCM|rcsb pdb:1GK6|rcsb pdb:1GZL|rcsb pdb:1IHQ|rcsb pdb:1IJ0|rcsb pdb:1IJ1|rcsb pdb:1IJ2|rcsb pdb:1IJ3|rcsb pdb:1KQL|rcsb pdb:1LD4|rcsb pdb:1LLM|rcsb pdb:1NKN|rcsb pdb:1PIQ|rcsb pdb:1RB4|rcsb pdb:1RB5|rcsb pdb:1RB6|rcsb pdb:1SWI|rcsb pdb:1TMZ|rcsb pdb:1UNT|rcsb pdb:1UNU|rcsb pdb:1UNV|rcsb pdb:1UNW|rcsb pdb:1UNX|rcsb pdb:1UNY|rcsb pdb:1UNZ|rcsb pdb:1UO0|rcsb pdb:1UO1|rcsb pdb:1UO2|rcsb pdb:1UO3|rcsb pdb:1UO4|rcsb pdb:1UO5|rcsb pdb:1W5G|rcsb pdb:1W5H|rcsb pdb:1W5I|rcsb pdb:1W5J|rcsb pdb:1W5K|rcsb pdb:1W5L|rcsb pdb:1YSA|rcsb pdb:1ZII|rcsb pdb:1ZIJ|rcsb pdb:1ZIK|rcsb pdb:1ZIL|rcsb pdb:1ZIM|rcsb pdb:1ZTA|rcsb pdb:2AHP|rcsb pdb:2B1F|rcsb pdb:2B22|rcsb pdb:2BNI|rcsb pdb:2CCE|rcsb pdb:2CCF|rcsb pdb:2CCN|rcsb pdb:2D3E|rcsb pdb:2DGC|rcsb pdb:2EFR|rcsb pdb:2EFS|rcsb pdb:2G9J|rcsb pdb:2HY6|rcsb pdb:2IPZ|rcsb pdb:2K8X|rcsb pdb:2LPB|rcsb pdb:2N9B|rcsb pdb:2NRN|rcsb pdb:2O7H|rcsb pdb:2OVN|rcsb pdb:2VKY|rcsb pdb:2VNL|rcsb pdb:2WG5|rcsb pdb:2WG6|rcsb pdb:2WPY|rcsb pdb:2WPZ|rcsb pdb:2WQ0|rcsb pdb:2WQ1|rcsb pdb:2WQ2|rcsb pdb:2WQ3|rcsb pdb:2YNY|rcsb pdb:2YNZ|rcsb pdb:2YO0|rcsb pdb:2YO1|rcsb pdb:2YO2|rcsb pdb:2YO3|rcsb pdb:2Z5H|rcsb pdb:2Z5I|rcsb pdb:2ZTA|rcsb pdb:3AZD|rcsb pdb:3BAS|rcsb pdb:3BAT|rcsb pdb:3CK4|rcsb pdb:3CRP|rcsb pdb:3G9R|rcsb pdb:3GJP|rcsb pdb:3I1G|rcsb pdb:3I5C|rcsb pdb:3K7Z|rcsb pdb:3M48|rcsb pdb:3P8M|rcsb pdb:3ZMF|rcsb pdb:4C46|rcsb pdb:4DMD|rcsb pdb:4G2K|rcsb pdb:4HU5|rcsb pdb:4HU6|rcsb pdb:4NIZ|rcsb pdb:4NJ0|rcsb pdb:4NJ1|rcsb pdb:4NJ2|rcsb pdb:4OWI|rcsb pdb:4TL1|rcsb pdb:5APP|rcsb pdb:5APQ|rcsb pdb:5APS|rcsb pdb:5APT|rcsb pdb:5APU|rcsb pdb:5APV|rcsb pdb:5APW|rcsb pdb:5APX|rcsb pdb:5APY|rcsb pdb:5APZ|rcsb pdb:5IEW|rcsb pdb:5IIR|rcsb pdb:5IIV|rcsb pdb:5KHT|rcsb pdb:6E52|rcsb pdb:6E95|rcsb pdb:6H9M|rcsb pdb:6HN4|rcsb pdb:6HN5|rcsb pdb:6O2E|rcsb pdb:6O2F|rcsb pdb:6PSA|rcsb pdb:6UT2|rcsb pdb:6VWG|rcsb pdb:6VWH|rcsb pdb:6VWI|rcsb pdb:6VWJ|reactome:R-SCE-2559580|reactome:R-SCE-2871796|reactome:R-SCE-450341	refseq:NP_009637.1|sgd:S000000285|ensemblfungi:YBR081C(gene)|ensemblfungi:YBR081C_mRNA(transcript)|go:"GO:0000124"(SAGA complex)|go:"GO:0000747"(conjugation with cellular fusion)|go:"GO:0005198"(structural molecule activity)|go:"GO:0005739"(mitochondrion)|go:"GO:0006325"(chromatin organization)|go:"GO:0006357"(regulation of transcription by RNA polymerase II)|go:"GO:0016573"(histone acetylation)|go:"GO:0034622"|go:"GO:0046695"("SLIK (SAGA-like) complex")|go:"GO:0046982"(protein heterodimerization activity)|interpro:IPR001487(Bromodomain)|interpro:IPR009072(Histone-fold)|interpro:IPR018359|interpro:IPR036427|interpro:IPR037782|rcsb pdb:6T9I|rcsb pdb:6T9K|dip:DIP-771N|mint:P35177	-	crc64:2ED1B8E35D509578	crc64:083B63624669244F	figure legend:f5e|comment:"we tested whether the lack of the Taf9 CRD  impaired the interaction of Gcn4 with the SAGA complex. GST  pulldown assays (9, 23) using GST-Gcn4 showed interaction of  Spt7, Gcn5, Tra1, Taf12, and Taf9 from the TAF9-HA3 extract in a  manner dependent on the WT Gcn4 activation domain (Fig. 5E).  As expected from our previous study (9), the SWI/SNF subunit  Snf6, used as a control here, was specifically pulled down. Whereas  no interaction with Taf1 was seen, interaction with TBP did not  show specificity for the Gcn4 activation domain, as observed previously  (9, 23). The pulldown assays conducted from the tCRD2-  HA3 extract also showed the pulldown of the same SAGA subunits,  albeit with reduced recovery of the Gcn5 subunit (Fig. 5E).  Importantly, the interaction of Gcn4 with Tra1 and Taf12 was not  impaired in the tCRD2-HA3 extract (Fig. 5E). Together, these results  indicate that the interactions of the Gcn4 activation domain  with the mutant SAGA complex are largely intact in vitro."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2015/05/19	2015/05/21	rogid:MFj9JcYccWKTayvnuaN95djsHd0559292	rogid:U3k+xA1SuVJRX240wH99s5ijrN0559292	rigid:h7fKB+ckA1LiSRUrDgYoc5WOKhE	false	glutathione s tranferase tag:?-?	-	-	-	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)