#ID(s) interactor A ID(s) interactor B Alt. ID(s) interactor A Alt. ID(s) interactor B Alias(es) interactor A Alias(es) interactor B Interaction detection method(s) Publication 1st author(s) Publication Identifier(s) Taxid interactor A Taxid interactor B Interaction type(s) Source database(s) Interaction identifier(s) Confidence value(s) Expansion method(s) Biological role(s) interactor A Biological role(s) interactor B Experimental role(s) interactor A Experimental role(s) interactor B Type(s) interactor A Type(s) interactor B Xref(s) interactor A Xref(s) interactor B Interaction Xref(s) Annotation(s) interactor A Annotation(s) interactor B Interaction annotation(s) Host organism(s) Interaction parameter(s) Creation date Update date Checksum(s) interactor A Checksum(s) interactor B Interaction Checksum(s) Negative Feature(s) interactor A Feature(s) interactor B Stoichiometry(s) interactor A Stoichiometry(s) interactor B Identification method participant A Identification method participant B uniprotkb:P77357 uniprotkb:P76052 intact:EBI-9155452|uniprotkb:P76847|ensemblbacteria:AAC74420|ensemblbacteria:BAA14940 intact:EBI-1123629|uniprotkb:Q2MBF2|ensemblbacteria:AAC74419|ensemblbacteria:BAE76404 psi-mi:abga_ecoli(display_long)|uniprotkb:abgA(gene name)|psi-mi:abgA(display_short)|uniprotkb:PABA-GLU hydrolase(gene name synonym)|uniprotkb:ydaJ(gene name synonym)|uniprotkb:JW5205(locus name)|uniprotkb:b1338(locus name) psi-mi:abgb_ecoli(display_long)|uniprotkb:abgB(gene name)|psi-mi:abgB(display_short)|uniprotkb:b1337(locus name)|uniprotkb:JW1331(locus name)|uniprotkb:ECK1333(orf name)|uniprotkb:ydaI(gene name synonym)|uniprotkb:PABA-GLU hydrolase(gene name synonym) psi-mi:"MI:0096"(pull down) Green et al. (2010) pubmed:20190044|imex:IM-23670 taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)") taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)") psi-mi:"MI:0915"(physical association) psi-mi:"MI:0469"(IntAct) intact:EBI-10053383|imex:IM-23670-1 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0498"(prey) psi-mi:"MI:0496"(bait) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) interpro:IPR002933(Peptidase M20)|interpro:IPR011650(Peptidase M20, dimerisation)|interpro:IPR017439(Peptidase M20D, mername-AA028/carboxypeptidase Ss1)|refseq:NP_415854.4|refseq:WP_000444929.1|interpro:IPR033845|interpro:IPR036264|ensemblbacteria:AAC74420(transcript)|ensemblbacteria:b1338(gene)|ensemblbacteria:BAA14940(gene)|ensemblbacteria:BAA14940(transcript)|go:"GO:0005737"(cytoplasm)|go:"GO:0016805"(dipeptidase activity)|go:"GO:0046657"(folic acid catabolic process)|go:"GO:0046982"(protein heterodimerization activity)|go:"GO:0071713"(para-aminobenzoyl-glutamate hydrolase activity) refseq:NP_415853.1|interpro:IPR017439(Peptidase M20D, mername-AA028/carboxypeptidase Ss1)|interpro:IPR017145(Aminobenzoyl-glutamate utilization protein B)|interpro:IPR002933(Peptidase M20)|refseq:WP_001156451.1|ensemblbacteria:AAC74419(transcript)|ensemblbacteria:b1337(gene)|ensemblbacteria:BAE76404(gene)|ensemblbacteria:BAE76404(transcript)|go:"GO:0005737"(cytoplasm)|go:"GO:0016805"(dipeptidase activity)|go:"GO:0046657"(folic acid catabolic process)|go:"GO:0046982"(protein heterodimerization activity)|go:"GO:0071713"(para-aminobenzoyl-glutamate hydrolase activity)|interpro:IPR036264 - - - comment:Stoichiometry determined by amino acid analysis.|figure legend:Fig. 4|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)") - 2015/01/08 2015/01/08 rogid:cWbrubYs0ulyvNw205CNH8Yu/Iw83333 rogid:BvNfFJCHUWJhGw6gSUUIoWofYaw83333 intact-crc:AEC2C4FAD1094216|rigid:RNAc0Ggj8CaQBfe8MIKBtNkj8p4 false - his tag:c-c 1 1 psi-mi:"MI:0433"(partial identification of protein sequence) psi-mi:"MI:0433"(partial identification of protein sequence) chebi:"CHEBI:60903" intenz:3.5.1 intact:EBI-10053528 intact:EBI-10053413 psi-mi:"n-(4-aminobenzoyl)-l-glutamate"(display_short)|psi-mi:"CHEBI:60903"(display_long)|intact:"(2S)-2-[(4-aminobenzoyl)amino]pentanedioate"(synonym)|intact:4-aminobenzoyl-glutamate(synonym)|intact:"N-(4-aminobenzoyl)-L-glutamate"(synonym)|intact:"N-(4-aminobenzoyl)-L-glutamic acid(2-)"(synonym)|intact:p-aminobenzoyl-glutamate(synonym)|intact:para-aminobenzoyl-glutamate(synonym)|intact:"N-(4-aminobenzoyl)-L-glutamate"(iupac name) psi-mi:abgab_ecoli(display_short)|psi-mi:3.5.1(display_long)|intact:p-aminobenzoyl-glutamate hydrolase complex(complex recommended name)|intact:"abgA:abgB"(complex systematic name)|intact:PABA-GLU hydrolase(complex synonym)|intact:PGH(complex synonym) psi-mi:"MI:0990"(cleavage assay) Green et al. (2010) pubmed:20190044|imex:IM-23670 taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)") taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)") psi-mi:"MI:0194"(cleavage reaction) psi-mi:"MI:0469"(IntAct) intact:EBI-10053527|imex:IM-23670-3 - - psi-mi:"MI:0502"(enzyme target) psi-mi:"MI:0501"(enzyme) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0328"(small molecule) psi-mi:"MI:1302"(stable complex) - evidence ontology:"ECO:0000353"|complex portal:CPX-28(complex-primary)|intact:EBI-10053396(exp-evidence)|pubmed:20190044(see-also)|go:"GO:0071713"(para-aminobenzoyl-glutamate hydrolase activity)|go:"GO:0046657"(folic acid catabolic process)|go:"GO:0030145"(manganese ion binding)|go:"GO:1902494"(catalytic complex) - inchi key:GADGMZDHLQLZRI-VIFPVBQESA-L curated-complex:"Catalyzes the cleavage of the folate breakdown product p-aminobenzoyl-glutamate (PABA-GLU) to form p-aminobenzoate (PABA) and glutamate. Reduced derivatives of folic acid are required for biosynthesis of DNA, RNA, amino acids, and other important cellular components."|complex-properties:Molecular weight = 206 kDa. A secondary 400-500 kDa species has also been observed. Km value for PABA-GLU = 60 uM, specific activity = 63,300 nmol/min/mg.|complex-assembly:Heterodimer figure legend:Figs 2,3|comment:Interaction was manganese dependent.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) km:6.0x10^-5(molar) 2015/01/08 2015/01/08 - - intact-crc:BA17774BDAC36B5A false - - - - psi-mi:"MI:0396"(predetermined participant) psi-mi:"MI:0396"(predetermined participant) uniprotkb:P77357 uniprotkb:P76052 intact:EBI-9155452|uniprotkb:P76847|ensemblbacteria:AAC74420|ensemblbacteria:BAA14940 intact:EBI-1123629|uniprotkb:Q2MBF2|ensemblbacteria:AAC74419|ensemblbacteria:BAE76404 psi-mi:abga_ecoli(display_long)|uniprotkb:abgA(gene name)|psi-mi:abgA(display_short)|uniprotkb:PABA-GLU hydrolase(gene name synonym)|uniprotkb:ydaJ(gene name synonym)|uniprotkb:JW5205(locus name)|uniprotkb:b1338(locus name) psi-mi:abgb_ecoli(display_long)|uniprotkb:abgB(gene name)|psi-mi:abgB(display_short)|uniprotkb:b1337(locus name)|uniprotkb:JW1331(locus name)|uniprotkb:ECK1333(orf name)|uniprotkb:ydaI(gene name synonym)|uniprotkb:PABA-GLU hydrolase(gene name synonym) psi-mi:"MI:0071"(molecular sieving) Green et al. (2010) pubmed:20190044|imex:IM-23670 taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)") taxid:83333(ecoli)|taxid:83333("Escherichia coli (strain K12)") psi-mi:"MI:0407"(direct interaction) psi-mi:"MI:0469"(IntAct) intact:EBI-10053396|imex:IM-23670-2 - - psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0497"(neutral component) psi-mi:"MI:0326"(protein) psi-mi:"MI:0326"(protein) interpro:IPR002933(Peptidase M20)|interpro:IPR011650(Peptidase M20, dimerisation)|interpro:IPR017439(Peptidase M20D, mername-AA028/carboxypeptidase Ss1)|refseq:NP_415854.4|refseq:WP_000444929.1|interpro:IPR033845|interpro:IPR036264|ensemblbacteria:AAC74420(transcript)|ensemblbacteria:b1338(gene)|ensemblbacteria:BAA14940(gene)|ensemblbacteria:BAA14940(transcript)|go:"GO:0005737"(cytoplasm)|go:"GO:0016805"(dipeptidase activity)|go:"GO:0046657"(folic acid catabolic process)|go:"GO:0046982"(protein heterodimerization activity)|go:"GO:0071713"(para-aminobenzoyl-glutamate hydrolase activity) refseq:NP_415853.1|interpro:IPR017439(Peptidase M20D, mername-AA028/carboxypeptidase Ss1)|interpro:IPR017145(Aminobenzoyl-glutamate utilization protein B)|interpro:IPR002933(Peptidase M20)|refseq:WP_001156451.1|ensemblbacteria:AAC74419(transcript)|ensemblbacteria:b1337(gene)|ensemblbacteria:BAE76404(gene)|ensemblbacteria:BAE76404(transcript)|go:"GO:0005737"(cytoplasm)|go:"GO:0016805"(dipeptidase activity)|go:"GO:0046657"(folic acid catabolic process)|go:"GO:0046982"(protein heterodimerization activity)|go:"GO:0071713"(para-aminobenzoyl-glutamate hydrolase activity)|interpro:IPR036264 - - - comment:Complex first purified by pulldown. Molecular mass of complex = 206 kDa secondary peak associated with a mass between 400 and 500 kDa also observed. SDS-PAGE revealed that fractions containing activity were composed of both AbgA and AbgB|figure legend:Fig. 5|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/01/08 2015/01/15 rogid:cWbrubYs0ulyvNw205CNH8Yu/Iw83333 rogid:BvNfFJCHUWJhGw6gSUUIoWofYaw83333 intact-crc:4F45A7CAB5CDA2D2|rigid:RNAc0Ggj8CaQBfe8MIKBtNkj8p4 false - his tag:c-c - - psi-mi:"MI:0396"(predetermined participant) psi-mi:"MI:0396"(predetermined participant)