#ID(s) interactor A ID(s) interactor B Alt. ID(s) interactor A Alt. ID(s) interactor B Alias(es) interactor A Alias(es) interactor B Interaction detection method(s) Publication 1st author(s) Publication Identifier(s) Taxid interactor A Taxid interactor B Interaction type(s) Source database(s) Interaction identifier(s) Confidence value(s) Expansion method(s) Biological role(s) interactor A Biological role(s) interactor B Experimental role(s) interactor A Experimental role(s) interactor B Type(s) interactor A Type(s) interactor B Xref(s) interactor A Xref(s) interactor B Interaction Xref(s) Annotation(s) interactor A Annotation(s) interactor B Interaction annotation(s) Host organism(s) Interaction parameter(s) Creation date Update date Checksum(s) interactor A Checksum(s) interactor B Interaction Checksum(s) Negative Feature(s) interactor A Feature(s) interactor B Stoichiometry(s) interactor A Stoichiometry(s) interactor B Identification method participant A Identification method participant B intact:EBI-11413715 uniprotkb:P0C074 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-970846|uniprotkb:D6W143|ensemblfungi:YNL138W-A psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:ysf3_yeast(display_long)|uniprotkb:YSF3(gene name)|psi-mi:YSF3(display_short)|uniprotkb:RCP10(gene name synonym)|uniprotkb:YNL138W-A(locus name)|uniprotkb:Splicing factor 3b subunit(gene name synonym) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616232|imex:IM-25038-1 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - go:"GO:0000398"(mRNA splicing, via spliceosome)|go:"GO:0005686"(U2 snRNP)|go:"GO:0071011"(precatalytic spliceosome)|ensemblfungi:YNL138W-A(gene)|ensemblfungi:YNL138W-A_mRNA(transcript)|go:"GO:0000245"(spliceosomal complex assembly)|refseq:NP_878153.1|sgd:S000028509|interpro:IPR009846(Splicing factor 3B subunit 5/RDS3 complex subunit 10)|rcsb pdb:7DCO|rcsb pdb:5GM6|rcsb pdb:5LQW|rcsb pdb:5ZWM|rcsb pdb:5ZWO|rcsb pdb:6G90|rcsb pdb:5NRL - - - figure legend:Fig 3, Table 1|comment:The spiceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate B complex 0.05mM ATP was added to stall the splicing reaction.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2016/04/01 - rogid:+ifBAgC9wh9hksXMFWXrcwu8eOU559292 - false tag:1-1|32p radiolabel:?-? - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:P54999 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-770|uniprotkb:D6W4I2|ensemblfungi:YPR182W psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:ruxf_yeast(display_long)|uniprotkb:SMX3(gene name)|psi-mi:SMX3(display_short)|uniprotkb:YPR182W(locus name)|uniprotkb:P9705.4(orf name)|uniprotkb:Sm protein F(gene name synonym) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616232|imex:IM-25038-1 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - refseq:NP_015508.1|sgd:S000006386|dip:DIP-1634N|mint:P54999|ensemblfungi:YPR182W(gene)|ensemblfungi:YPR182W_mRNA(transcript)|go:"GO:0000387"(spliceosomal snRNP assembly)|go:"GO:0000398"(mRNA splicing, via spliceosome)|go:"GO:0003723"(RNA binding)|go:"GO:0005682"(U5 snRNP)|go:"GO:0005685"(U1 snRNP)|go:"GO:0005686"(U2 snRNP)|go:"GO:0008266"("poly(U) RNA binding")|go:"GO:0046540"(U4/U6 x U5 tri-snRNP complex)|go:"GO:0071013"(catalytic step 2 spliceosome)|go:"GO:1990935"(splicing factor binding)|interpro:IPR001163(Like-Sm ribonucleoprotein, core)|interpro:IPR010920(Like-Sm ribonucleoprotein-related, core)|interpro:IPR016487(Small nuclear ribonucleoprotein SmF)|interpro:IPR034100|rcsb pdb:1N9R|rcsb pdb:1N9S|rcsb pdb:3JCM|rcsb pdb:5GAM|rcsb pdb:5GAN|rcsb pdb:5GAO|rcsb pdb:5GM6|rcsb pdb:5GMK|rcsb pdb:5LJ3|rcsb pdb:5LJ5|rcsb pdb:5LQW|rcsb pdb:5MPS|rcsb pdb:5MQ0|rcsb pdb:5NRL|rcsb pdb:5WSG|rcsb pdb:5Y88|rcsb pdb:5YLZ|rcsb pdb:5ZWM|rcsb pdb:5ZWN|rcsb pdb:5ZWO|rcsb pdb:6BK8|rcsb pdb:6EXN|rcsb pdb:6G90|rcsb pdb:6J6G|rcsb pdb:6J6H|rcsb pdb:6J6N|rcsb pdb:6J6Q|rcsb pdb:6N7P|rcsb pdb:6N7R|rcsb pdb:6N7X|rcsb pdb:7B9V - - crc64:1640D3237597B2D8 figure legend:Fig 3, Table 1|comment:The spiceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate B complex 0.05mM ATP was added to stall the splicing reaction.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2016/04/01 - rogid:iyBbVNf2fgOIqhnOg9Etn3P2r3Q559292 - false tag:1-1|32p radiolabel:?-? - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:P20053 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-219|uniprotkb:D6W4H8|ensemblfungi:YPR178W psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:prp4_yeast(display_long)|uniprotkb:PRP4(gene name)|psi-mi:PRP4(display_short)|uniprotkb:RNA4(gene name synonym)|uniprotkb:YPR178W(locus name)|uniprotkb:P9705.6(orf name)|uniprotkb:Pre-mRNA-processing protein 4(gene name synonym) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616232|imex:IM-25038-1 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - refseq:NP_015504.1|sgd:S000006382|mint:P20053|dip:DIP-2607N|ensemblfungi:YPR178W(gene)|ensemblfungi:YPR178W_mRNA(transcript)|go:"GO:0000393"(spliceosomal conformational changes to generate catalytic conformation)|go:"GO:0000398"(mRNA splicing, via spliceosome)|go:"GO:0005681"(spliceosomal complex)|go:"GO:0017070"(U6 snRNA binding)|go:"GO:0030621"(U4 snRNA binding)|go:"GO:0034247"(snoRNA splicing)|go:"GO:0046540"(U4/U6 x U5 tri-snRNP complex)|interpro:IPR001680(WD40 repeat)|interpro:IPR014906("Pre-mRNA processing factor 4 (PRP4) like")|interpro:IPR015943(WD40/YVTN repeat-like)|interpro:IPR019775|interpro:IPR020472|interpro:IPR036322|rcsb pdb:3JCM|rcsb pdb:5GAN|rcsb pdb:5GAP|rcsb pdb:5NRL|rcsb pdb:5ZWM|rcsb pdb:5ZWO - - crc64:7CCCA3A92AC0CE78 figure legend:Fig 3, Table 1|comment:The spiceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate B complex 0.05mM ATP was added to stall the splicing reaction.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2016/04/01 - rogid:vvz5G0rqqyrugTvAPelDKit9FpQ559292 - false tag:1-1|32p radiolabel:?-? - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:Q06525 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-35138|uniprotkb:D6W4E8|ensemblfungi:YPR152C psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:urn1_yeast(display_long)|uniprotkb:URN1(gene name)|psi-mi:URN1(display_short)|uniprotkb:U2-U5-U6 snRNP, RES complex and NTC-interacting pre-mRNA-splicing factor 1(gene name synonym)|uniprotkb:YPR152C(locus name) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616232|imex:IM-25038-1 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - refseq:NP_015478.1|sgd:S000006356|mint:Q06525|dip:DIP-2017N|ensemblfungi:YPR152C(gene)|ensemblfungi:YPR152C_mRNA(transcript)|go:"GO:0000398"(mRNA splicing, via spliceosome)|go:"GO:0003723"(RNA binding)|go:"GO:0005634"(nucleus)|go:"GO:0005685"(U1 snRNP)|go:"GO:0071004"(U2-type prespliceosome)|go:"GO:0071010"(prespliceosome)|interpro:IPR001202(WW/Rsp5/WWP)|interpro:IPR002713(FF)|interpro:IPR036020|interpro:IPR036517|rcsb pdb:2JUC - - - figure legend:Fig 3, Table 1|comment:The spiceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate B complex 0.05mM ATP was added to stall the splicing reaction.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2016/04/01 - rogid:DJmq8bwerafwnFLeSGjF+0hTpPE559292 - false tag:1-1|32p radiolabel:?-? - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:Q06091 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-818|uniprotkb:D6W499|ensemblfungi:YPR101W psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:sn309_yeast(display_long)|uniprotkb:YPR101W(locus name)|uniprotkb:SNT309(gene name)|psi-mi:SNT309(display_short)|uniprotkb:NTC25(gene name synonym)|uniprotkb:Synergistic to PRP19 mutation protein 309(gene name synonym)|uniprotkb:PRP19-associated complex protein 25(gene name synonym) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616232|imex:IM-25038-1 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - sgd:S000006305|refseq:NP_015426.1|dip:DIP-2864N|mint:Q06091|ensemblfungi:YPR101W(gene)|ensemblfungi:YPR101W_mRNA(transcript)|go:"GO:0000398"(mRNA splicing, via spliceosome)|go:"GO:0000974"(Prp19 complex)|go:"GO:0071006"(U2-type catalytic step 1 spliceosome)|rcsb pdb:5GM6|rcsb pdb:5GMK|rcsb pdb:5LJ5|rcsb pdb:5MQ0|rcsb pdb:5WSG|rcsb pdb:5Y88|rcsb pdb:5YLZ|rcsb pdb:6BK8|rcsb pdb:6EXN|rcsb pdb:6J6G|rcsb pdb:6J6H|rcsb pdb:6J6N|rcsb pdb:6J6Q - - crc64:B781CA65BC9A1918 figure legend:Fig 3, Table 1|comment:The spiceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate B complex 0.05mM ATP was added to stall the splicing reaction.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2016/04/01 - rogid:ELyyYlQDmNRzWKQx/63xvHuAG8M559292 - false tag:1-1|32p radiolabel:?-? - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:Q06819 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-166|ensemblfungi:YPR082C|uniprotkb:D6W484 psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:dib1_yeast(display_long)|uniprotkb:DIB1(gene name)|psi-mi:DIB1(display_short)|uniprotkb:CDH1(gene name synonym)|uniprotkb:SNU16(gene name synonym)|uniprotkb:YPR082C(locus name)|uniprotkb:P9513.12(orf name) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616232|imex:IM-25038-1 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - refseq:NP_015407.1|sgd:S000006286|ensemblfungi:YPR082C(gene)|ensemblfungi:YPR082C_mRNA(transcript)|go:"GO:0000398"(mRNA splicing, via spliceosome)|go:"GO:0005681"(spliceosomal complex)|go:"GO:0005682"(U5 snRNP)|go:"GO:0046540"(U4/U6 x U5 tri-snRNP complex)|interpro:IPR004123(mRNA splicing factor, thioredoxin-like U5 snRNP)|interpro:IPR036249|rcsb pdb:3JCM|rcsb pdb:5GAN|rcsb pdb:5GAP|rcsb pdb:5NRL|rcsb pdb:5ZWM|rcsb pdb:5ZWO|dip:DIP-1289N - - crc64:B8C11278571C57E5 figure legend:Fig 3, Table 1|comment:The spiceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate B complex 0.05mM ATP was added to stall the splicing reaction.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2016/04/01 - rogid:mRibIpinKPlRNdh60VJj/Z0tjUc559292 - false tag:1-1|32p radiolabel:?-? - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:Q08963 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-594|uniprotkb:D6W3F7|ensemblfungi:YPL213W psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:ru2a_yeast(display_long)|uniprotkb:YPL213W(locus name)|uniprotkb:LEA1(gene name)|psi-mi:LEA1(display_short)|uniprotkb:Looks exceptionally like U2A protein 1(gene name synonym) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616232|imex:IM-25038-1 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - refseq:NP_015111.1|sgd:S000006134|dip:DIP-2399N|mint:Q08963|ensemblfungi:YPL213W(gene)|ensemblfungi:YPL213W_mRNA(transcript)|go:"GO:0000398"(mRNA splicing, via spliceosome)|go:"GO:0005686"(U2 snRNP)|go:"GO:0005737"(cytoplasm)|go:"GO:0030620"(U2 snRNA binding)|go:"GO:0071004"(U2-type prespliceosome)|interpro:IPR001611(Leucine-rich repeat)|interpro:IPR003603(U2A'/phosphoprotein 32 family A, C-terminal)|interpro:IPR032675|interpro:IPR044640|rcsb pdb:5GMK|rcsb pdb:5LJ3|rcsb pdb:5LJ5|rcsb pdb:5MQ0|rcsb pdb:5NRL|rcsb pdb:5WSG|rcsb pdb:5Y88|rcsb pdb:5YLZ|rcsb pdb:5ZWM|rcsb pdb:5ZWO|rcsb pdb:6BK8|rcsb pdb:6EXN|rcsb pdb:6G90|rcsb pdb:6J6G|rcsb pdb:6J6H|rcsb pdb:6J6N|rcsb pdb:6J6Q - - - figure legend:Fig 3, Table 1|comment:The spiceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate B complex 0.05mM ATP was added to stall the splicing reaction.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2016/04/01 - rogid:PlKwD+gRYbhUBE4GcxTaP0wt8zM559292 - false tag:1-1|32p radiolabel:?-? - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:Q12417 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-710|uniprotkb:D6W3L8|ensemblfungi:YPL151C psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:prp46_yeast(display_long)|uniprotkb:CWC1(gene name synonym)|uniprotkb:PRP nineteen-associated complex protein 50(gene name synonym)|uniprotkb:PRP19-associated complex protein 50(gene name synonym)|uniprotkb:P2594(orf name)|uniprotkb:YPL151C(locus name)|uniprotkb:PRP46(gene name)|psi-mi:PRP46(display_short)|uniprotkb:NTC50(gene name synonym)|uniprotkb:Pre-mRNA-processing protein 46(gene name synonym)|uniprotkb:Complexed with CEF1 protein 1(gene name synonym) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616232|imex:IM-25038-1 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - refseq:NP_015174.1|sgd:S000006072|ensemblfungi:YPL151C(gene)|ensemblfungi:YPL151C_mRNA(transcript)|go:"GO:0000398"(mRNA splicing, via spliceosome)|go:"GO:0000974"(Prp19 complex)|go:"GO:0005681"(spliceosomal complex)|go:"GO:0005737"(cytoplasm)|go:"GO:0071013"(catalytic step 2 spliceosome)|go:"GO:0080008"(Cul4-RING E3 ubiquitin ligase complex)|interpro:IPR001680(WD40 repeat)|interpro:IPR015943(WD40/YVTN repeat-like)|interpro:IPR019775|interpro:IPR020472|interpro:IPR036322|dip:DIP-3994N|mint:Q12417|rcsb pdb:5GM6|rcsb pdb:5GMK|rcsb pdb:5LJ3|rcsb pdb:5LJ5|rcsb pdb:5LQW|rcsb pdb:5MPS|rcsb pdb:5MQ0|rcsb pdb:5WSG|rcsb pdb:5Y88|rcsb pdb:5YLZ|rcsb pdb:6BK8|rcsb pdb:6EXN|rcsb pdb:6J6G|rcsb pdb:6J6H|rcsb pdb:6J6N|rcsb pdb:6J6Q - - crc64:0E888F566A5414BD figure legend:Fig 3, Table 1|comment:The spiceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate B complex 0.05mM ATP was added to stall the splicing reaction.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2016/04/01 - rogid:016ynVER9V3d44NwkqRG7TIvf9w559292 - false tag:1-1|32p radiolabel:?-? - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:Q99181 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-8579|uniprotkb:D6W317|ensemblfungi:YOR319W psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:hsh49_yeast(display_long)|uniprotkb:O6142(orf name)|uniprotkb:YOR319W(locus name)|uniprotkb:HSH49(gene name)|psi-mi:HSH49(display_short) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616232|imex:IM-25038-1 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - refseq:NP_014964.1|sgd:S000005846|mint:Q99181|dip:DIP-930N|ensemblfungi:YOR319W(gene)|ensemblfungi:YOR319W_mRNA(transcript)|go:"GO:0000398"(mRNA splicing, via spliceosome)|go:"GO:0003723"(RNA binding)|go:"GO:0003730"(mRNA 3'-UTR binding)|go:"GO:0005634"(nucleus)|go:"GO:0005686"(U2 snRNP)|go:"GO:0005829"(cytosol)|go:"GO:0008143"("poly(A) binding")|go:"GO:0008266"("poly(U) RNA binding")|go:"GO:0071004"(U2-type prespliceosome)|go:"GO:1990904"(ribonucleoprotein complex)|interpro:IPR000504(RNA recognition motif, RNP-1)|interpro:IPR012677(Nucleotide-binding, alpha-beta plait)|interpro:IPR034158|interpro:IPR035979|rcsb pdb:5GM6|rcsb pdb:5LSB|rcsb pdb:5LSL|rcsb pdb:5NRL|rcsb pdb:5ZWM|rcsb pdb:5ZWO|rcsb pdb:6G90 - - crc64:F6FC44083094B176 figure legend:Fig 3, Table 1|comment:The spiceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate B complex 0.05mM ATP was added to stall the splicing reaction.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2016/04/01 - rogid:LWcxvibHcl0Oyi1OidqUtMnOLP8559292 - false tag:1-1|32p radiolabel:?-? - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:Q12420 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-260|uniprotkb:D6W308|ensemblfungi:YOR308C psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:snu66_yeast(display_long)|uniprotkb:SNU66(gene name)|psi-mi:SNU66(display_short)|uniprotkb:05667(orf name)|uniprotkb:YOR308C(locus name) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616232|imex:IM-25038-1 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - sgd:S000005835|refseq:NP_014953.3|mint:Q12420|dip:DIP-5216N|ensemblfungi:YOR308C(gene)|ensemblfungi:YOR308C_mRNA(transcript)|go:"GO:0000398"(mRNA splicing, via spliceosome)|go:"GO:0000481"(maturation of 5S rRNA)|go:"GO:0005634"(nucleus)|go:"GO:0005681"(spliceosomal complex)|go:"GO:0045292"(mRNA cis splicing, via spliceosome)|go:"GO:0046540"(U4/U6 x U5 tri-snRNP complex)|interpro:IPR005011(SART-1 protein)|rcsb pdb:3PLU|rcsb pdb:3PLV|rcsb pdb:5GAN|rcsb pdb:5GAO|rcsb pdb:5NRL|rcsb pdb:5ZWM|rcsb pdb:5ZWO - - crc64:154BC3873DD6B0FA figure legend:Fig 3, Table 1|comment:The spiceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate B complex 0.05mM ATP was added to stall the splicing reaction.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2016/04/01 - rogid:+h6+o43YeiJlMZ1bVF45QdZ8Y00559292 - false tag:1-1|32p radiolabel:?-? - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:Q12330 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-16359|uniprotkb:D6W2L6|ensemblfungi:YOR159C psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:ruxe_yeast(display_long)|uniprotkb:SME1(gene name)|psi-mi:SME1(display_short)|uniprotkb:YOR159C(locus name)|uniprotkb:Sm protein E(gene name synonym) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616232|imex:IM-25038-1 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - ensemblfungi:YOR159C(gene)|ensemblfungi:YOR159C_mRNA(transcript)|go:"GO:0000387"(spliceosomal snRNP assembly)|go:"GO:0000398"(mRNA splicing, via spliceosome)|go:"GO:0003723"(RNA binding)|go:"GO:0005682"(U5 snRNP)|go:"GO:0005685"(U1 snRNP)|go:"GO:0005686"(U2 snRNP)|go:"GO:0005687"(U4 snRNP)|go:"GO:0005737"(cytoplasm)|go:"GO:0046540"(U4/U6 x U5 tri-snRNP complex)|go:"GO:0071004"(U2-type prespliceosome)|go:"GO:0071011"(precatalytic spliceosome)|go:"GO:1990935"(splicing factor binding)|interpro:IPR001163(Like-Sm ribonucleoprotein, core)|interpro:IPR010920(Like-Sm ribonucleoprotein-related, core)|interpro:IPR027078|rcsb pdb:3JCM|rcsb pdb:5GAM|rcsb pdb:5GAN|rcsb pdb:5GAO|rcsb pdb:5GM6|rcsb pdb:5GMK|sgd:S000005685|rcsb pdb:5LJ3|rcsb pdb:5LJ5|rcsb pdb:5LQW|rcsb pdb:5MPS|rcsb pdb:5MQ0|rcsb pdb:5NRL|rcsb pdb:5WSG|rcsb pdb:5Y88|rcsb pdb:5YLZ|rcsb pdb:5ZWM|rcsb pdb:5ZWN|rcsb pdb:5ZWO|rcsb pdb:6BK8|rcsb pdb:6EXN|rcsb pdb:6G90|rcsb pdb:6J6G|rcsb pdb:6J6H|rcsb pdb:6J6N|rcsb pdb:6J6Q|rcsb pdb:6N7P|rcsb pdb:6N7R|rcsb pdb:6N7X|rcsb pdb:7B9V|refseq:NP_014802.3|dip:DIP-1633N|mint:Q12330 - - crc64:B76D72DA8456F3B5 figure legend:Fig 3, Table 1|comment:The spiceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate B complex 0.05mM ATP was added to stall the splicing reaction.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2016/04/01 - rogid:IiV/AXz4zJjC7A2iuBixxiLYo9E559292 - false tag:1-1|32p radiolabel:?-? - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:P53905 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-141|uniprotkb:D6W135|ensemblfungi:YNL147W psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:lsm7_yeast(display_long)|uniprotkb:LSM7(gene name)|psi-mi:LSM7(display_short)|uniprotkb:N1202(orf name)|uniprotkb:N1780(orf name)|uniprotkb:YNL147W(locus name) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616232|imex:IM-25038-1 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - refseq:NP_014252.2|sgd:S000005091|mint:P53905|dip:DIP-1419N|go:"GO:0005689"(U12-type spliceosomal complex)|go:"GO:0005730"(nucleolus)|go:"GO:0000956"(nuclear-transcribed mRNA catabolic process)|go:"GO:0003723"(RNA binding)|go:"GO:0005634"(nucleus)|go:"GO:0005688"(U6 snRNP)|ensemblfungi:YNL147W(gene)|ensemblfungi:YNL147W_mRNA(transcript)|go:"GO:0000398"(mRNA splicing, via spliceosome)|go:"GO:0005732"("sno(s)RNA-containing ribonucleoprotein complex")|go:"GO:0005737"(cytoplasm)|go:"GO:0008033"(tRNA processing)|go:"GO:0030490"(maturation of SSU-rRNA)|go:"GO:0046540"(U4/U6 x U5 tri-snRNP complex)|go:"GO:0071004"(U2-type prespliceosome)|go:"GO:0071013"(catalytic step 2 spliceosome)|go:"GO:0097526"(spliceosomal tri-snRNP complex)|go:"GO:1990726"(Lsm1-7-Pat1 complex)|go:"GO:1990904"(ribonucleoprotein complex)|interpro:IPR001163(Like-Sm ribonucleoprotein, core)|interpro:IPR010920(Like-Sm ribonucleoprotein-related, core)|interpro:IPR017132(U6 snRNA-associated Sm-like protein LSm7)|interpro:IPR044641|rcsb pdb:3JCM|rcsb pdb:4C8Q|rcsb pdb:4C92|rcsb pdb:4M75|rcsb pdb:4M77|rcsb pdb:4M78|rcsb pdb:4M7A|rcsb pdb:4M7D|rcsb pdb:5GAN|rcsb pdb:5NRL|rcsb pdb:5VSU|rcsb pdb:5ZWM|rcsb pdb:5ZWO|rcsb pdb:6ASO|reactome:R-SCE-430039 - - crc64:EA1D019FC5D1B5C5 figure legend:Fig 3, Table 1|comment:The spiceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate B complex 0.05mM ATP was added to stall the splicing reaction.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2016/04/01 - rogid:LBD0KzUVuBCAGeCVPV5bSS8Hodg559292 - false tag:1-1|32p radiolabel:?-? - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:P49955 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-664|uniprotkb:D6W0B5|ensemblfungi:YMR288W psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:sf3b1_yeast(display_long)|uniprotkb:YMR288W(locus name)|uniprotkb:YM8021.14(orf name)|uniprotkb:HSH155(gene name)|psi-mi:HSH155(display_short) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616232|imex:IM-25038-1 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - refseq:NP_014015.1|sgd:S000004901|dip:DIP-2628N|mint:P49955|ensemblfungi:YMR288W(gene)|ensemblfungi:YMR288W_mRNA(transcript)|go:"GO:0000245"(spliceosomal complex assembly)|go:"GO:0000398"(mRNA splicing, via spliceosome)|go:"GO:0003729"(mRNA binding)|go:"GO:0005686"(U2 snRNP)|go:"GO:0071004"(U2-type prespliceosome)|go:"GO:0071013"(catalytic step 2 spliceosome)|interpro:IPR011989(Armadillo-like helical)|interpro:IPR016024(Armadillo-type fold)|interpro:IPR038737|rcsb pdb:5GM6|rcsb pdb:5LQW|rcsb pdb:5NRL|rcsb pdb:5ZWM|rcsb pdb:5ZWO|rcsb pdb:6G90 - - crc64:27D26E4252A788E2 figure legend:Fig 3, Table 1|comment:The spiceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate B complex 0.05mM ATP was added to stall the splicing reaction.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2016/04/01 - rogid:t0fwQOOum/AO/xL8vhZdF2EGymo559292 - false tag:1-1|32p radiolabel:?-? - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:Q02554 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-654|uniprotkb:Q04012|uniprotkb:D6W066|ensemblfungi:YMR240C psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:cus1_yeast(display_long)|uniprotkb:YM9408.02C(orf name)|uniprotkb:YMR240C(locus name)|uniprotkb:CUS1(gene name)|psi-mi:CUS1(display_short) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616232|imex:IM-25038-1 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - refseq:NP_013967.1|sgd:S000004853|dip:DIP-910N|mint:Q02554|ensemblfungi:YMR240C(gene)|ensemblfungi:YMR240C_mRNA(transcript)|go:"GO:0000245"(spliceosomal complex assembly)|go:"GO:0000398"(mRNA splicing, via spliceosome)|go:"GO:0003723"(RNA binding)|go:"GO:0005684"(U2-type spliceosomal complex)|go:"GO:0005686"(U2 snRNP)|go:"GO:0071004"(U2-type prespliceosome)|go:"GO:0071011"(precatalytic spliceosome)|go:"GO:0071013"(catalytic step 2 spliceosome)|interpro:IPR006568(PSP, proline-rich)|interpro:IPR007180(Protein of unknown function DUF382)|rcsb pdb:5GM6|rcsb pdb:5LSB|rcsb pdb:5LSL|rcsb pdb:5NRL|rcsb pdb:5ZWM|rcsb pdb:5ZWO|rcsb pdb:6G90 - - crc64:0204B3A666AF7336 figure legend:Fig 3, Table 1|comment:The spiceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate B complex 0.05mM ATP was added to stall the splicing reaction.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2016/04/01 - rogid:4LtSdsLH2EISJjPGM78b73TD0yw559292 - false tag:1-1|32p radiolabel:?-? - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:Q03654 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-476|uniprotkb:Q6B1D8|ensemblfungi:YMR213W|uniprotkb:D6W038 psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:cef1_yeast(display_long)|uniprotkb:CEF1(gene name)|psi-mi:CEF1(display_short)|uniprotkb:YMR213W(locus name)|uniprotkb:YM8261.07(orf name)|uniprotkb:NTC85(gene name synonym)|uniprotkb:PRP nineteen-associated complex protein 85(gene name synonym)|uniprotkb:PRP19-associated complex protein 85(gene name synonym) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616232|imex:IM-25038-1 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - refseq:NP_013940.1|sgd:S000004826|ensemblfungi:YMR213W(gene)|ensemblfungi:YMR213W_mRNA(transcript)|go:"GO:0000350"(generation of catalytic spliceosome for second transesterification step)|go:"GO:0000398"(mRNA splicing, via spliceosome)|go:"GO:0000974"(Prp19 complex)|go:"GO:0003677"(DNA binding)|go:"GO:0005681"(spliceosomal complex)|go:"GO:0005737"(cytoplasm)|go:"GO:0071006"(U2-type catalytic step 1 spliceosome)|interpro:IPR001005(SANT, DNA-binding)|interpro:IPR009057(Homeodomain-like)|interpro:IPR017930|interpro:IPR021786|rcsb pdb:5GM6|rcsb pdb:5GMK|rcsb pdb:5LJ3|rcsb pdb:5LJ5|rcsb pdb:5LQW|rcsb pdb:5MPS|rcsb pdb:5MQ0|rcsb pdb:5WSG|rcsb pdb:5Y88|rcsb pdb:5YLZ|rcsb pdb:6BK8|rcsb pdb:6EXN|rcsb pdb:6J6G|rcsb pdb:6J6H|rcsb pdb:6J6N|rcsb pdb:6J6Q|rcsb pdb:7DCO|dip:DIP-1113N|mint:Q03654 - - crc64:A4C417A8B330EA9C figure legend:Fig 3, Table 1|comment:The spiceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate B complex 0.05mM ATP was added to stall the splicing reaction.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2016/04/01 - rogid:AsbV7RtNk+8mCzLk2wZYqbULOFg559292 - false tag:1-1|32p radiolabel:?-? - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:Q04693 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-519|ensemblfungi:YML049C|uniprotkb:D6VZC6 psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:rse1_yeast(display_long)|uniprotkb:RSE1(gene name)|psi-mi:RSE1(display_short)|uniprotkb:YML049C(locus name)|uniprotkb:YM9827.03C(orf name)|uniprotkb:SAP130(gene name synonym)|uniprotkb:RNA splicing and ER to Golgi transport factor 1(gene name synonym)|uniprotkb:Spliceosome-associated protein 130(gene name synonym) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616232|imex:IM-25038-1 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - refseq:NP_013663.1|sgd:S000004513|ensemblfungi:YML049C(gene)|ensemblfungi:YML049C_mRNA(transcript)|go:"GO:0000245"(spliceosomal complex assembly)|go:"GO:0000398"(mRNA splicing, via spliceosome)|go:"GO:0005634"(nucleus)|go:"GO:0005686"(U2 snRNP)|go:"GO:0030620"(U2 snRNA binding)|go:"GO:0071004"(U2-type prespliceosome)|interpro:IPR004871(Cleavage and polyadenylation specificity factor, A subunit, C-terminal)|interpro:IPR015943(WD40/YVTN repeat-like)|interpro:IPR018846|rcsb pdb:5GM6|rcsb pdb:5LQW|rcsb pdb:5NRL|rcsb pdb:5ZWM|rcsb pdb:5ZWO|rcsb pdb:6G90|dip:DIP-856N|mint:Q04693 - - crc64:4D92837F5C267D67 figure legend:Fig 3, Table 1|comment:The spiceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate B complex 0.05mM ATP was added to stall the splicing reaction.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2016/04/01 - rogid:L2J86R3nXgMd8CE/6Yd2jxA/sx4559292 - false tag:1-1|32p radiolabel:?-? - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:P39682 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-612|uniprotkb:D6VZC9|uniprotkb:E9P8Z1|ensemblfungi:YML046W psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:prp39_yeast(display_long)|uniprotkb:YM9827.06(orf name)|uniprotkb:YML046W(locus name)|uniprotkb:PRP39(gene name)|psi-mi:PRP39(display_short) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616232|imex:IM-25038-1 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - refseq:NP_013667.1|sgd:S000004509|dip:DIP-812N|mint:P39682|ensemblfungi:YML046W(gene)|ensemblfungi:YML046W_mRNA(transcript)|go:"GO:0000243"(commitment complex)|go:"GO:0000395"(mRNA 5'-splice site recognition)|go:"GO:0005685"(U1 snRNP)|go:"GO:0071004"(U2-type prespliceosome)|interpro:IPR003107(RNA-processing protein, HAT helix)|interpro:IPR011990(Tetratricopeptide-like helical)|rcsb pdb:5ZWN|rcsb pdb:6G90|rcsb pdb:6N7P|rcsb pdb:6N7R|rcsb pdb:6N7X - - - figure legend:Fig 3, Table 1|comment:The spiceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate B complex 0.05mM ATP was added to stall the splicing reaction.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2016/04/01 - rogid:urVLzQFKnYT1bYOs3yRd9dd7lMk559292 - false tag:1-1|32p radiolabel:?-? - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:Q06217 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-235|uniprotkb:D6VYS2|ensemblfungi:YLR275W psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:smd2_yeast(display_long)|uniprotkb:SMD2(gene name)|psi-mi:SMD2(display_short)|uniprotkb:YLR275W(locus name)|uniprotkb:L9328.5(orf name)|uniprotkb:snRNP core protein D2(gene name synonym) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616232|imex:IM-25038-1 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - sgd:S000004265|refseq:NP_013377.1|mint:Q06217|dip:DIP-1421N|ensemblfungi:YLR275W(gene)|ensemblfungi:YLR275W_mRNA(transcript)|go:"GO:0000387"(spliceosomal snRNP assembly)|go:"GO:0003723"(RNA binding)|go:"GO:0005682"(U5 snRNP)|go:"GO:0005685"(U1 snRNP)|go:"GO:0005686"(U2 snRNP)|go:"GO:0046540"(U4/U6 x U5 tri-snRNP complex)|go:"GO:0071004"(U2-type prespliceosome)|go:"GO:0071011"(precatalytic spliceosome)|go:"GO:0071013"(catalytic step 2 spliceosome)|interpro:IPR001163(Like-Sm ribonucleoprotein, core)|interpro:IPR010920(Like-Sm ribonucleoprotein-related, core)|interpro:IPR027248|rcsb pdb:3JCM|rcsb pdb:5GAM|rcsb pdb:5GAN|rcsb pdb:5GAO|rcsb pdb:5GM6|rcsb pdb:5GMK|rcsb pdb:5LJ3|rcsb pdb:5LJ5|rcsb pdb:5LQW|rcsb pdb:5MPS|rcsb pdb:5MQ0|rcsb pdb:5NRL|rcsb pdb:5WSG|rcsb pdb:5Y88|rcsb pdb:5YLZ|rcsb pdb:5ZWM|rcsb pdb:5ZWN|rcsb pdb:5ZWO|rcsb pdb:6BK8|rcsb pdb:6EXN|rcsb pdb:6G90|rcsb pdb:6J6G|rcsb pdb:6J6H|rcsb pdb:6J6N|rcsb pdb:6J6Q|rcsb pdb:6N7P|rcsb pdb:6N7R|rcsb pdb:6N7X|rcsb pdb:7B9V - - crc64:7287070B10E090C0 figure legend:Fig 3, Table 1|comment:The spiceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate B complex 0.05mM ATP was added to stall the splicing reaction.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2016/04/01 - rogid:rglZ3AQuJfa1rFYIeZLqiYj+L4U559292 - false tag:1-1|32p radiolabel:?-? - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:P43321 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-529|ensemblfungi:YLR147C|uniprotkb:D6VYE2|uniprotkb:E9P8W3 psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:smd3_yeast(display_long)|uniprotkb:SMD3(gene name)|psi-mi:SMD3(display_short)|uniprotkb:YLR147C(locus name)|uniprotkb:L9634.6(orf name)|uniprotkb:snRNP core protein D3(gene name synonym) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616232|imex:IM-25038-1 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - refseq:NP_013248.1|sgd:S000004137|rcsb pdb:5YLZ|rcsb pdb:5ZWM|rcsb pdb:5ZWN|rcsb pdb:5ZWO|rcsb pdb:6BK8|rcsb pdb:6EXN|rcsb pdb:6G90|rcsb pdb:6J6G|rcsb pdb:6J6H|rcsb pdb:6J6N|rcsb pdb:6J6Q|rcsb pdb:6N7P|rcsb pdb:6N7R|rcsb pdb:6N7X|rcsb pdb:7B9V|ensemblfungi:YLR147C(gene)|ensemblfungi:YLR147C_mRNA(transcript)|go:"GO:0000243"(commitment complex)|go:"GO:0000387"(spliceosomal snRNP assembly)|go:"GO:0000398"(mRNA splicing, via spliceosome)|go:"GO:0003723"(RNA binding)|go:"GO:0003729"(mRNA binding)|go:"GO:0005682"(U5 snRNP)|go:"GO:0005685"(U1 snRNP)|go:"GO:0005686"(U2 snRNP)|go:"GO:0005687"(U4 snRNP)|go:"GO:0005689"(U12-type spliceosomal complex)|go:"GO:0034715"(pICln-Sm protein complex)|go:"GO:0034719"(SMN-Sm protein complex)|go:"GO:0046540"(U4/U6 x U5 tri-snRNP complex)|go:"GO:0071004"(U2-type prespliceosome)|go:"GO:0071011"(precatalytic spliceosome)|go:"GO:0071013"(catalytic step 2 spliceosome)|go:"GO:0097526"(spliceosomal tri-snRNP complex)|interpro:IPR001163(Like-Sm ribonucleoprotein, core)|interpro:IPR010920(Like-Sm ribonucleoprotein-related, core)|interpro:IPR027141|interpro:IPR034099|rcsb pdb:3JCM|rcsb pdb:5GAM|rcsb pdb:5GAN|rcsb pdb:5GAO|rcsb pdb:5GM6|rcsb pdb:5GMK|rcsb pdb:5LJ3|rcsb pdb:5LJ5|rcsb pdb:5LQW|rcsb pdb:5MPS|rcsb pdb:5MQ0|rcsb pdb:5NRL|rcsb pdb:5WSG|rcsb pdb:5Y88|dip:DIP-715N|mint:P43321 - - crc64:BB02A56C5AD70CF2 figure legend:Fig 3, Table 1|comment:The spiceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate B complex 0.05mM ATP was added to stall the splicing reaction.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2016/04/01 - rogid:ueJ4D76t8ilvz/p9qMzIGiCzqWk559292 - false tag:1-1|32p radiolabel:?-? - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:Q12309 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-484|uniprotkb:D6VYB5|ensemblfungi:YLR117C psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:clf1_yeast(display_long)|uniprotkb:CLF1(gene name)|psi-mi:CLF1(display_short)|uniprotkb:NTC77(gene name synonym)|uniprotkb:YLR117C(locus name)|uniprotkb:SYF3(gene name synonym)|uniprotkb:L2952(orf name)|uniprotkb:Crooked neck-like factor 1(gene name synonym)|uniprotkb:PRP19-associated complex protein 77(gene name synonym)|uniprotkb:Synthetic lethal with CDC40 protein 3(gene name synonym) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616232|imex:IM-25038-1 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - refseq:NP_013218.1|sgd:S000004107|rcsb pdb:6J6H|rcsb pdb:6J6N|rcsb pdb:6J6Q|rcsb pdb:5GM6|rcsb pdb:5GMK|rcsb pdb:5LJ3|rcsb pdb:5LJ5|rcsb pdb:5LQW|rcsb pdb:5MPS|rcsb pdb:5MQ0|rcsb pdb:5WSG|rcsb pdb:5Y88|rcsb pdb:5YLZ|rcsb pdb:6BK8|rcsb pdb:6EXN|rcsb pdb:6J6G|ensemblfungi:YLR117C(gene)|ensemblfungi:YLR117C_mRNA(transcript)|go:"GO:0000245"(spliceosomal complex assembly)|go:"GO:0000354"(cis assembly of pre-catalytic spliceosome)|go:"GO:0000398"(mRNA splicing, via spliceosome)|go:"GO:0000785"(chromatin)|go:"GO:0000974"(Prp19 complex)|go:"GO:0003682"(chromatin binding)|go:"GO:0003688"(DNA replication origin binding)|go:"GO:0006270"(DNA replication initiation)|go:"GO:0071004"(U2-type prespliceosome)|go:"GO:0071006"(U2-type catalytic step 1 spliceosome)|go:"GO:0071007"(U2-type catalytic step 2 spliceosome)|go:"GO:0071008"(U2-type post-mRNA release spliceosomal complex)|go:"GO:0071014"(post-mRNA release spliceosomal complex)|interpro:IPR003107(RNA-processing protein, HAT helix)|interpro:IPR011990(Tetratricopeptide-like helical)|dip:DIP-1685N|mint:Q12309 - - crc64:AE7D5EC5B3979E00 figure legend:Fig 3, Table 1|comment:The spiceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate B complex 0.05mM ATP was added to stall the splicing reaction.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2016/04/01 - rogid:VbNHO9YGUushIXHTgscFu4xCzC4559292 - false tag:1-1|32p radiolabel:?-? - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:P32523 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-493|uniprotkb:Q07870|ensemblfungi:YLL036C|uniprotkb:D6VXW9 psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:prp19_yeast(display_long)|uniprotkb:PRP19(gene name)|psi-mi:PRP19(display_short)|uniprotkb:PSO4(gene name synonym)|uniprotkb:RING-type E3 ubiquitin transferase PRP19(gene name synonym)|uniprotkb:YLL036C(locus name) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616232|imex:IM-25038-1 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - refseq:NP_013064.1|sgd:S000003959|rcsb pdb:6EXN|rcsb pdb:6J6G|rcsb pdb:6J6H|rcsb pdb:6J6N|rcsb pdb:6J6Q|reactome:R-SCE-6781823|reactome:R-SCE-6782135|reactome:R-SCE-6782210|interpro:IPR015943(WD40/YVTN repeat-like)|interpro:IPR038959|rcsb pdb:1N87|rcsb pdb:2BAY|rcsb pdb:3LRV|rcsb pdb:4ZB4|rcsb pdb:5GM6|rcsb pdb:5GMK|rcsb pdb:5LJ5|rcsb pdb:5M8C|rcsb pdb:5MQ0|ensemblfungi:YLL036C(gene)|ensemblfungi:YLL036C_mRNA(transcript)|go:"GO:0000349"(generation of catalytic spliceosome for first transesterification step)|go:"GO:0000974"(Prp19 complex)|go:"GO:0004842"(ubiquitin-protein transferase activity)|go:"GO:0005634"(nucleus)|go:"GO:0005737"(cytoplasm)|go:"GO:0005739"(mitochondrion)|go:"GO:0005829"(cytosol)|go:"GO:0006281"(DNA repair)|go:"GO:0042802"(identical protein binding)|go:"GO:0061630"(ubiquitin protein ligase activity)|go:"GO:0070534"(protein K63-linked ubiquitination)|go:"GO:0071006"(U2-type catalytic step 1 spliceosome)|go:"GO:0071013"(catalytic step 2 spliceosome)|interpro:IPR003613(U box)|interpro:IPR011047(Quinonprotein alcohol dehydrogenase-like)|interpro:IPR013083(Zinc finger, RING/FYVE/PHD-type)|interpro:IPR013915(Pre-mRNA-splicing factor 19)|rcsb pdb:5WSG|rcsb pdb:5Y88|rcsb pdb:5YLZ|rcsb pdb:6BK8|dip:DIP-1112N|mint:P32523 - - crc64:A44D8C7DD6F64185 figure legend:Fig 3, Table 1|comment:The spiceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate B complex 0.05mM ATP was added to stall the splicing reaction.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2016/04/01 - rogid:/oQc2rJZ7WcCIx7cnqE6XSW/3Vk559292 - false tag:1-1|32p radiolabel:?-? - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:P21374 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-9382|ensemblfungi:YJR050W|uniprotkb:D6VWM1 psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:isy1_yeast(display_long)|uniprotkb:ISY1(gene name)|psi-mi:ISY1(display_short)|uniprotkb:UTR3(gene name synonym)|uniprotkb:YJR050W(locus name)|uniprotkb:J1657(orf name)|uniprotkb:NTC30(gene name synonym)|uniprotkb:Interactor of SYF1(gene name synonym)|uniprotkb:PRP19-associated complex protein 30(gene name synonym) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616232|imex:IM-25038-1 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - ensemblfungi:YJR050W(gene)|ensemblfungi:YJR050W_mRNA(transcript)|go:"GO:0000350"(generation of catalytic spliceosome for second transesterification step)|go:"GO:0000389"(mRNA 3'-splice site recognition)|go:"GO:0000974"(Prp19 complex)|go:"GO:0005737"(cytoplasm)|go:"GO:0071006"(U2-type catalytic step 1 spliceosome)|go:"GO:0071007"(U2-type catalytic step 2 spliceosome)|go:"GO:0071013"(catalytic step 2 spliceosome)|go:"GO:0071014"(post-mRNA release spliceosomal complex)|go:"GO:0071020"(post-spliceosomal complex)|interpro:IPR009360(Isy1-like splicing)|interpro:IPR029012|interpro:IPR037200|rcsb pdb:5GMK|rcsb pdb:5LJ3|rcsb pdb:5LJ5|rcsb pdb:6J6G|rcsb pdb:6J6H|rcsb pdb:6J6N|rcsb pdb:6J6Q|reactome:R-SCE-6781823|reactome:R-SCE-6782135|reactome:R-SCE-6782210|sgd:S000003811|refseq:NP_012584.3|mint:P21374|dip:DIP-1682N - - - figure legend:Fig 3, Table 1|comment:The spiceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate B complex 0.05mM ATP was added to stall the splicing reaction.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2016/04/01 - rogid:NHLAtd+wLxwtWQD8PsyzyQsfJOg559292 - false tag:1-1|32p radiolabel:?-? - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:P47093 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-313|uniprotkb:D6VWJ6|ensemblfungi:YJR022W psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:lsm8_yeast(display_long)|uniprotkb:LSM8(gene name)|psi-mi:LSM8(display_short)|uniprotkb:J1464(orf name)|uniprotkb:YJR83.16(orf name)|uniprotkb:YJR022W(locus name) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616232|imex:IM-25038-1 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - refseq:NP_012556.2|sgd:S000003783|ensemblfungi:YJR022W(gene)|ensemblfungi:YJR022W_mRNA(transcript)|go:"GO:0000398"(mRNA splicing, via spliceosome)|go:"GO:0003723"(RNA binding)|go:"GO:0005634"(nucleus)|go:"GO:0005688"(U6 snRNP)|go:"GO:0005730"(nucleolus)|go:"GO:0005737"(cytoplasm)|go:"GO:0006364"(rRNA processing)|go:"GO:0008033"(tRNA processing)|go:"GO:0016070"(RNA metabolic process)|go:"GO:0046540"(U4/U6 x U5 tri-snRNP complex)|go:"GO:0071011"(precatalytic spliceosome)|interpro:IPR001163(Like-Sm ribonucleoprotein, core)|interpro:IPR010920(Like-Sm ribonucleoprotein-related, core)|interpro:IPR034103|interpro:IPR044642|dip:DIP-903N|rcsb pdb:3JCM|rcsb pdb:4M77|rcsb pdb:4M78|rcsb pdb:4M7A|rcsb pdb:4M7D|rcsb pdb:5GAN|rcsb pdb:5NRL|rcsb pdb:5VSU|rcsb pdb:5ZWM|rcsb pdb:5ZWO|rcsb pdb:6ASO|mint:P47093 - - crc64:B6E37F585B6292EA figure legend:Fig 3, Table 1|comment:The spiceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate B complex 0.05mM ATP was added to stall the splicing reaction.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2016/04/01 - rogid:+X6fjvb8flf36BcPKxtclUwNMSY559292 - false tag:1-1|32p radiolabel:?-? - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:P32524 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-603|intact:EBI-464832|uniprotkb:Q6Q5G7|uniprotkb:D6VVZ1|ensemblfungi:YJL203W psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:prp21_yeast(display_long)|uniprotkb:PRP21(gene name)|psi-mi:PRP21(display_short)|uniprotkb:SPP91(gene name synonym)|uniprotkb:YJL203W(locus name)|uniprotkb:J0322(orf name) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616232|imex:IM-25038-1 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - refseq:NP_012332.1|sgd:S000003739|dip:DIP-689N|mint:P32524|ensemblfungi:YJL203W(gene)|ensemblfungi:YJL203W_mRNA(transcript)|go:"GO:0000398"(mRNA splicing, via spliceosome)|go:"GO:0003723"(RNA binding)|go:"GO:0005686"(U2 snRNP)|go:"GO:0071004"(U2-type prespliceosome)|go:"GO:0071013"(catalytic step 2 spliceosome)|interpro:IPR000061(SWAP/Surp)|interpro:IPR022030|interpro:IPR035967|rcsb pdb:4DGW|rcsb pdb:5NRL|rcsb pdb:5ZWM|rcsb pdb:5ZWO|rcsb pdb:6G90|rcsb pdb:7DCO - - crc64:2E9DBA6F06759B3F figure legend:Fig 3, Table 1|comment:The spiceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate B complex 0.05mM ATP was added to stall the splicing reaction.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2016/04/01 - rogid:mOta2u2zOwtuZB+8s4z9DBL9iI0559292 - false tag:1-1|32p radiolabel:?-? - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:P53277 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-23308|uniprotkb:D6VUR1|ensemblfungi:YGR129W psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:syf2_yeast(display_long)|uniprotkb:SYF2(gene name)|psi-mi:SYF2(display_short)|uniprotkb:NTC31(gene name synonym)|uniprotkb:Synthetic lethal with CDC40 protein 2(gene name synonym)|uniprotkb:PRP19 complex protein 31(gene name synonym)|uniprotkb:YGR129W(locus name) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616232|imex:IM-25038-1 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - sgd:S000003361|refseq:NP_011645.3|dip:DIP-1684N|mint:P53277|interpro:IPR013260(mRNA splicing factor SYF2)|ensemblfungi:YGR129W(gene)|rcsb pdb:5GM6|rcsb pdb:5GMK|rcsb pdb:5MPS|rcsb pdb:5MQ0|rcsb pdb:5WSG|rcsb pdb:5Y88|rcsb pdb:5YLZ|rcsb pdb:6BK8|rcsb pdb:6EXN|rcsb pdb:6J6G|rcsb pdb:6J6H|rcsb pdb:6J6N|rcsb pdb:6J6Q|ensemblfungi:YGR129W_mRNA(transcript)|go:"GO:0000398"(mRNA splicing, via spliceosome)|go:"GO:0000974"(Prp19 complex)|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0071004"(U2-type prespliceosome)|go:"GO:0071006"(U2-type catalytic step 1 spliceosome)|go:"GO:0071007"(U2-type catalytic step 2 spliceosome)|go:"GO:0071008"(U2-type post-mRNA release spliceosomal complex) - - - figure legend:Fig 3, Table 1|comment:The spiceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate B complex 0.05mM ATP was added to stall the splicing reaction.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2016/04/01 - rogid:uVAPXi98XAE2k7IPXvd4d+KNknw559292 - false tag:1-1|32p radiolabel:?-? - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:P49704 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-347|uniprotkb:Q66R99|uniprotkb:D6VUM3|ensemblfungi:YGR091W psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:prp31_yeast(display_long)|uniprotkb:PRP31(gene name)|psi-mi:PRP31(display_short)|uniprotkb:YGR091W(locus name) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616232|imex:IM-25038-1 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - refseq:NP_011605.1|sgd:S000003323|dip:DIP-5355N|mint:P49704|ensemblfungi:YGR091W(gene)|ensemblfungi:YGR091W_mRNA(transcript)|go:"GO:0000244"(spliceosomal tri-snRNP complex assembly)|go:"GO:0000387"(spliceosomal snRNP assembly)|go:"GO:0000398"(mRNA splicing, via spliceosome)|go:"GO:0003723"(RNA binding)|go:"GO:0005687"(U4 snRNP)|go:"GO:0005739"(mitochondrion)|go:"GO:0046540"(U4/U6 x U5 tri-snRNP complex)|go:"GO:0071011"(precatalytic spliceosome)|go:"GO:0097526"(spliceosomal tri-snRNP complex)|interpro:IPR002687(Pre-mRNA processing ribonucleoprotein, binding region)|interpro:IPR012976(NOSIC)|interpro:IPR019175|interpro:IPR027105|interpro:IPR036070|interpro:IPR042239|rcsb pdb:3JCM|rcsb pdb:5GAN|rcsb pdb:5GAP|rcsb pdb:5NRL|rcsb pdb:5ZWM|rcsb pdb:5ZWO - - crc64:E39AC6247859FB3B figure legend:Fig 3, Table 1|comment:The spiceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate B complex 0.05mM ATP was added to stall the splicing reaction.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2016/04/01 - rogid:H41JrfockxRzf+U5GKVgGxBTrW0559292 - false tag:1-1|32p radiolabel:?-? - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:Q00723 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-841|uniprotkb:D6VUK7|ensemblfungi:YGR075C psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:prp38_yeast(display_long)|uniprotkb:YGR075C(locus name)|uniprotkb:PRP38(gene name)|psi-mi:PRP38(display_short)|uniprotkb:Pre-mRNA-processing factor 38(gene name synonym) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616232|imex:IM-25038-1 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - sgd:S000003307|refseq:NP_011589.3|dip:DIP-1648N|mint:Q00723|ensemblfungi:YGR075C(gene)|ensemblfungi:YGR075C_mRNA(transcript)|go:"GO:0000388"("spliceosome conformational change to release U4 (or U4atac) and U1 (or U11)")|go:"GO:0046540"(U4/U6 x U5 tri-snRNP complex)|go:"GO:0071011"(precatalytic spliceosome)|interpro:IPR005037(PRP38)|rcsb pdb:5NRL|rcsb pdb:5ZWO - - crc64:62EB225947AF4349 figure legend:Fig 3, Table 1|comment:The spiceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate B complex 0.05mM ATP was added to stall the splicing reaction.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2016/04/01 - rogid:2C+lYBvjAO0jl1CAy0k1Q+Qv7ew559292 - false tag:1-1|32p radiolabel:?-? - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:Q02260 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-585|uniprotkb:D6VUK6|ensemblfungi:YGR074W psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:smd1_yeast(display_long)|uniprotkb:YGR074W(locus name)|uniprotkb:SMD1(gene name)|psi-mi:SMD1(display_short)|uniprotkb:snRNP core protein D1(gene name synonym) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616232|imex:IM-25038-1 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - sgd:S000003306|refseq:NP_011588.3|dip:DIP-227N|mint:Q02260|ensemblfungi:YGR074W(gene)|ensemblfungi:YGR074W_mRNA(transcript)|go:"GO:0000243"(commitment complex)|go:"GO:0000387"(spliceosomal snRNP assembly)|go:"GO:0000398"(mRNA splicing, via spliceosome)|go:"GO:0003723"(RNA binding)|go:"GO:0003729"(mRNA binding)|go:"GO:0005634"(nucleus)|go:"GO:0005682"(U5 snRNP)|go:"GO:0005685"(U1 snRNP)|go:"GO:0005686"(U2 snRNP)|go:"GO:0005689"(U12-type spliceosomal complex)|go:"GO:0005829"(cytosol)|go:"GO:0030532"(small nuclear ribonucleoprotein complex)|go:"GO:0034715"(pICln-Sm protein complex)|go:"GO:0005687"(U4 snRNP)|go:"GO:0034719"(SMN-Sm protein complex)|go:"GO:0046540"(U4/U6 x U5 tri-snRNP complex)|go:"GO:0071004"(U2-type prespliceosome)|go:"GO:0071011"(precatalytic spliceosome)|go:"GO:0071013"(catalytic step 2 spliceosome)|go:"GO:0097526"(spliceosomal tri-snRNP complex)|interpro:IPR001163(Like-Sm ribonucleoprotein, core)|interpro:IPR010920(Like-Sm ribonucleoprotein-related, core)|interpro:IPR027141|rcsb pdb:3JCM|rcsb pdb:5GAM|rcsb pdb:5GAN|rcsb pdb:5GAO|rcsb pdb:5GM6|rcsb pdb:5GMK|rcsb pdb:5LJ3|rcsb pdb:5LJ5|rcsb pdb:5LQW|rcsb pdb:5MPS|rcsb pdb:5MQ0|rcsb pdb:5NRL|rcsb pdb:5WSG|rcsb pdb:5Y88|rcsb pdb:5YLZ|rcsb pdb:5ZWM|rcsb pdb:5ZWN|rcsb pdb:5ZWO|rcsb pdb:6BK8|rcsb pdb:6EXN|rcsb pdb:6G90|rcsb pdb:6J6G|rcsb pdb:6J6H|rcsb pdb:6J6N|rcsb pdb:6J6Q|rcsb pdb:6N7P|rcsb pdb:6N7R|rcsb pdb:6N7X|rcsb pdb:7B9V - - crc64:9929F21EB9218447 figure legend:Fig 3, Table 1|comment:The spiceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate B complex 0.05mM ATP was added to stall the splicing reaction.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2016/04/01 - rogid:SvOKDbY05xgJXKZ8uS+keXsC41I559292 - false tag:1-1|32p radiolabel:?-? - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:P53207 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-736|uniprotkb:D6VUE9|ensemblfungi:YGR013W psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:snu71_yeast(display_long)|uniprotkb:YGR013W(locus name)|uniprotkb:SNU71(gene name)|psi-mi:SNU71(display_short) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616232|imex:IM-25038-1 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - refseq:NP_011527.1|sgd:S000003245|dip:DIP-5507N|mint:P53207|ensemblfungi:YGR013W(gene)|ensemblfungi:YGR013W_mRNA(transcript)|go:"GO:0000398"(mRNA splicing, via spliceosome)|go:"GO:0003723"(RNA binding)|go:"GO:0005685"(U1 snRNP)|go:"GO:0005737"(cytoplasm)|go:"GO:0071004"(U2-type prespliceosome)|interpro:IPR002483(Splicing factor PWI)|rcsb pdb:5ZWN|rcsb pdb:6G90|rcsb pdb:6N7P|rcsb pdb:6N7R|rcsb pdb:6N7X - - crc64:2659AAA62C4993D5 figure legend:Fig 3, Table 1|comment:The spiceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate B complex 0.05mM ATP was added to stall the splicing reaction.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2016/04/01 - rogid:/kqpx5vNl2twhBp8xThkSEXX8Wo559292 - false tag:1-1|32p radiolabel:?-? - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:P46947 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-24073|uniprotkb:D6VTX8|ensemblfungi:YGL174W psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:cwc26_yeast(display_long)|uniprotkb:YGL174W(locus name)|uniprotkb:G1642(orf name)|uniprotkb:BUD13(gene name)|psi-mi:BUD13(display_short)|uniprotkb:SLC7(gene name synonym)|uniprotkb:Complexed with CEF1 protein 26(gene name synonym)|uniprotkb:Bud site selection protein 13(gene name synonym)|uniprotkb:Synthetic lethal with CLF1 protein 7(gene name synonym)|uniprotkb:CWC26(gene name synonym) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616232|imex:IM-25038-1 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - sgd:S000003142|refseq:NP_011341.3|dip:DIP-2075N|mint:P46947|ensemblfungi:YGL174W(gene)|ensemblfungi:YGL174W_mRNA(transcript)|go:"GO:0000398"(mRNA splicing, via spliceosome)|go:"GO:0005634"(nucleus)|go:"GO:0005684"(U2-type spliceosomal complex)|go:"GO:0005737"(cytoplasm)|go:"GO:0070274"(RES complex)|interpro:IPR018609|rcsb pdb:2MKC|rcsb pdb:2MY2|rcsb pdb:4UQT|rcsb pdb:5GM6|rcsb pdb:5LQW|rcsb pdb:5ZWM|rcsb pdb:5ZWO - - crc64:1584B390121325CD figure legend:Fig 3, Table 1|comment:The spiceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate B complex 0.05mM ATP was added to stall the splicing reaction.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2016/04/01 - rogid:JGmiLd4cWl/n2wZVMju48AdYsNk559292 - false tag:1-1|32p radiolabel:?-? - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:P40204 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-637|uniprotkb:D6VTL2|ensemblfungi:YFL017W-A psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:ruxg_yeast(display_long)|uniprotkb:SNP2(gene name synonym)|uniprotkb:YFL017Bw(orf name)|uniprotkb:YFL017W-A(locus name)|uniprotkb:SMX2(gene name)|psi-mi:SMX2(display_short)|uniprotkb:Sm protein G(gene name synonym) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616232|imex:IM-25038-1 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - refseq:NP_116636.1|sgd:S000002965|dip:DIP-6683N|mint:P40204|ensemblfungi:YFL017W-A(gene)|ensemblfungi:YFL017W-A_mRNA(transcript)|go:"GO:0000387"(spliceosomal snRNP assembly)|go:"GO:0000398"(mRNA splicing, via spliceosome)|go:"GO:0003723"(RNA binding)|go:"GO:0005682"(U5 snRNP)|go:"GO:0005685"(U1 snRNP)|go:"GO:0005686"(U2 snRNP)|go:"GO:0005687"(U4 snRNP)|go:"GO:0005689"(U12-type spliceosomal complex)|go:"GO:0034719"(SMN-Sm protein complex)|go:"GO:0046540"(U4/U6 x U5 tri-snRNP complex)|go:"GO:0071004"(U2-type prespliceosome)|go:"GO:0071011"(precatalytic spliceosome)|go:"GO:0071013"(catalytic step 2 spliceosome)|go:"GO:0097526"(spliceosomal tri-snRNP complex)|go:"GO:1990904"(ribonucleoprotein complex)|interpro:IPR001163(Like-Sm ribonucleoprotein, core)|interpro:IPR010920(Like-Sm ribonucleoprotein-related, core)|interpro:IPR034098|interpro:IPR044641|rcsb pdb:3JCM|rcsb pdb:5GAM|rcsb pdb:5GAN|rcsb pdb:5GAO|rcsb pdb:5GM6|rcsb pdb:5GMK|rcsb pdb:5LJ3|rcsb pdb:5LJ5|rcsb pdb:5LQW|rcsb pdb:5MPS|rcsb pdb:5MQ0|rcsb pdb:5NRL|rcsb pdb:5WSG|rcsb pdb:5Y88|rcsb pdb:5YLZ|rcsb pdb:5ZWM|rcsb pdb:5ZWN|rcsb pdb:5ZWO|rcsb pdb:6BK8|rcsb pdb:6EXN|rcsb pdb:6G90|rcsb pdb:6J6G|rcsb pdb:6J6H|rcsb pdb:6J6N|rcsb pdb:6J6Q|rcsb pdb:6N7P|rcsb pdb:6N7R|rcsb pdb:6N7X|rcsb pdb:7B9V|rcsb pdb:7DCO - - crc64:5C6208B8C5A9AB33 figure legend:Fig 3, Table 1|comment:The spiceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate B complex 0.05mM ATP was added to stall the splicing reaction.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2016/04/01 - rogid:Wh37rGoSJFpJ5o+CuNkBnpiu/5s559292 - false tag:1-1|32p radiolabel:?-? - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:Q03338 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-421|uniprotkb:D6VT97|ensemblfungi:YDR473C psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:prp3_yeast(display_long)|uniprotkb:PRP3(gene name)|psi-mi:PRP3(display_short)|uniprotkb:RNA3(gene name synonym)|uniprotkb:YDR473C(locus name)|uniprotkb:D8035.16(orf name)|uniprotkb:Pre-mRNA-splicing factor 3(gene name synonym) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616232|imex:IM-25038-1 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - sgd:S000002881|refseq:NP_010761.3|ensemblfungi:YDR473C(gene)|ensemblfungi:YDR473C_mRNA(transcript)|go:"GO:0000387"(spliceosomal snRNP assembly)|go:"GO:0000398"(mRNA splicing, via spliceosome)|go:"GO:0005681"(spliceosomal complex)|go:"GO:0046540"(U4/U6 x U5 tri-snRNP complex)|interpro:IPR010541(Protein of unknown function DUF1115)|interpro:IPR013881(Pre-mRNA-splicing factor 3)|interpro:IPR027104|rcsb pdb:3JCM|rcsb pdb:4YHU|rcsb pdb:4YHV|rcsb pdb:4YHW|rcsb pdb:5GAN|rcsb pdb:5GAP|rcsb pdb:5NRL|rcsb pdb:5ZWM|rcsb pdb:5ZWO|dip:DIP-5266N|mint:Q03338 - - crc64:E977FD9F708BDD91 figure legend:Fig 3, Table 1|comment:The spiceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate B complex 0.05mM ATP was added to stall the splicing reaction.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2016/04/01 - rogid:KE0Y/kRXTq9LXt4PGsKnRcY7Sqg559292 - false tag:1-1|32p radiolabel:?-? - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:Q04048 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-540|uniprotkb:D6VT47|ensemblfungi:YDR416W psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:syf1_yeast(display_long)|uniprotkb:SYF1(gene name)|psi-mi:SYF1(display_short)|uniprotkb:NTC90(gene name synonym)|uniprotkb:Synthetic lethal with CDC40 protein 1(gene name synonym)|uniprotkb:PRP19-associated complex protein 90(gene name synonym)|uniprotkb:YDR416W(locus name) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616232|imex:IM-25038-1 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - refseq:NP_010704.1|sgd:S000002824|rcsb pdb:5YLZ|rcsb pdb:6BK8|rcsb pdb:6EXN|rcsb pdb:6J6G|rcsb pdb:6J6H|rcsb pdb:6J6N|rcsb pdb:6J6Q|reactome:R-SCE-6781823|reactome:R-SCE-6782135|reactome:R-SCE-6782210|rcsb pdb:5Y88|ensemblfungi:YDR416W(gene)|ensemblfungi:YDR416W_mRNA(transcript)|go:"GO:0000349"(generation of catalytic spliceosome for first transesterification step)|go:"GO:0000398"(mRNA splicing, via spliceosome)|go:"GO:0000974"(Prp19 complex)|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0071004"(U2-type prespliceosome)|go:"GO:0071006"(U2-type catalytic step 1 spliceosome)|go:"GO:0071007"(U2-type catalytic step 2 spliceosome)|go:"GO:0071008"(U2-type post-mRNA release spliceosomal complex)|go:"GO:0071014"(post-mRNA release spliceosomal complex)|interpro:IPR003107(RNA-processing protein, HAT helix)|interpro:IPR011990(Tetratricopeptide-like helical)|rcsb pdb:5GM6|rcsb pdb:5GMK|rcsb pdb:5LJ3|rcsb pdb:5LJ5|rcsb pdb:5LQW|rcsb pdb:5MPS|rcsb pdb:5MQ0|rcsb pdb:5WSG|dip:DIP-1681N|mint:Q04048 - - crc64:ED4368A624A40E7C figure legend:Fig 3, Table 1|comment:The spiceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate B complex 0.05mM ATP was added to stall the splicing reaction.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2016/04/01 - rogid:+1TwiqsQv1IcWxuoIWcJGtTCUFU559292 - false tag:1-1|32p radiolabel:?-? - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:Q06406 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-196|uniprotkb:D6VT10|ensemblfungi:YDR378C psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:lsm6_yeast(display_long)|uniprotkb:LSM6(gene name)|psi-mi:LSM6(display_short)|uniprotkb:D9481.18(orf name)|uniprotkb:YDR378C(locus name) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616232|imex:IM-25038-1 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - refseq:NP_010666.2|sgd:S000002786|mint:Q06406|dip:DIP-1418N|ensemblfungi:YDR378C(gene)|ensemblfungi:YDR378C_mRNA(transcript)|go:"GO:0000398"(mRNA splicing, via spliceosome)|go:"GO:0000932"(P-body)|go:"GO:0003723"(RNA binding)|go:"GO:0005681"(spliceosomal complex)|go:"GO:0005688"(U6 snRNP)|go:"GO:0005730"(nucleolus)|go:"GO:0005732"("sno(s)RNA-containing ribonucleoprotein complex")|go:"GO:0008033"(tRNA processing)|go:"GO:0030490"(maturation of SSU-rRNA)|go:"GO:0046540"(U4/U6 x U5 tri-snRNP complex)|go:"GO:1990726"(Lsm1-7-Pat1 complex)|interpro:IPR001163(Like-Sm ribonucleoprotein, core)|interpro:IPR010920(Like-Sm ribonucleoprotein-related, core)|interpro:IPR016487(Small nuclear ribonucleoprotein SmF)|rcsb pdb:3JCM|rcsb pdb:4C8Q|rcsb pdb:4C92|rcsb pdb:4M75|rcsb pdb:4M77|rcsb pdb:4M78|rcsb pdb:4M7A|rcsb pdb:4M7D|rcsb pdb:5GAN|rcsb pdb:5NRL|rcsb pdb:5VSU|rcsb pdb:5ZWM|rcsb pdb:5ZWO|rcsb pdb:6ASO|reactome:R-SCE-430039 - - crc64:74FE40A4509CEF33 figure legend:Fig 3, Table 1|comment:The spiceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate B complex 0.05mM ATP was added to stall the splicing reaction.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2016/04/01 - rogid:JQtrhOkngO6APIPCFMRnKogB96c559292 - false tag:1-1|32p radiolabel:?-? - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:Q03782 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-627|uniprotkb:D6VSM1|ensemblfungi:YDR240C psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:snu56_yeast(display_long)|uniprotkb:YDR240C(locus name)|uniprotkb:SNU56(gene name)|psi-mi:SNU56(display_short)|uniprotkb:MUD10(gene name synonym) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616232|imex:IM-25038-1 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - sgd:S000002648|refseq:NP_010526.1|dip:DIP-6514N|mint:Q03782|ensemblfungi:YDR240C(gene)|ensemblfungi:YDR240C_mRNA(transcript)|go:"GO:0000243"(commitment complex)|go:"GO:0000398"(mRNA splicing, via spliceosome)|go:"GO:0003729"(mRNA binding)|go:"GO:0005685"(U1 snRNP)|go:"GO:0071004"(U2-type prespliceosome)|interpro:IPR043954|rcsb pdb:5ZWN|rcsb pdb:6G90|rcsb pdb:6N7P|rcsb pdb:6N7R|rcsb pdb:6N7X - - crc64:5E0C7A1FD577808F figure legend:Fig 3, Table 1|comment:The spiceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate B complex 0.05mM ATP was added to stall the splicing reaction.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2016/04/01 - rogid:gz3aUh/8pFF7Yn8XS9gdhSWrVNs559292 - false tag:1-1|32p radiolabel:?-? - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:Q03776 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-802|uniprotkb:D6VSL6|ensemblfungi:YDR235W psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:prp42_yeast(display_long)|uniprotkb:PRP42(gene name)|psi-mi:PRP42(display_short)|uniprotkb:MUD16(gene name synonym)|uniprotkb:SNU65(gene name synonym)|uniprotkb:YD8419.02(orf name)|uniprotkb:YDR235W(locus name)|uniprotkb:Pre-mRNA-processing factor 42(gene name synonym)|uniprotkb:65 kDa snRNP protein(gene name synonym) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616232|imex:IM-25038-1 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - sgd:S000002643|refseq:NP_010521.1|dip:DIP-2836N|mint:Q03776|ensemblfungi:YDR235W(gene)|ensemblfungi:YDR235W_mRNA(transcript)|go:"GO:0000243"(commitment complex)|go:"GO:0000395"(mRNA 5'-splice site recognition)|go:"GO:0000398"(mRNA splicing, via spliceosome)|go:"GO:0003723"(RNA binding)|go:"GO:0005685"(U1 snRNP)|go:"GO:0071004"(U2-type prespliceosome)|interpro:IPR003107(RNA-processing protein, HAT helix)|interpro:IPR011990(Tetratricopeptide-like helical)|rcsb pdb:5ZWN|rcsb pdb:6G90|rcsb pdb:6N7P|rcsb pdb:6N7R|rcsb pdb:6N7X - - - figure legend:Fig 3, Table 1|comment:The spiceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate B complex 0.05mM ATP was added to stall the splicing reaction.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2016/04/01 - rogid:eYeDKyEQBF/JyFBIWmgx0Hlg1dU559292 - false tag:1-1|32p radiolabel:?-? - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:Q12368 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-252|uniprotkb:D6VRQ2|ensemblfungi:YDL098C psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:snu23_yeast(display_long)|uniprotkb:SNU23(gene name)|psi-mi:SNU23(display_short)|uniprotkb:D2378(orf name)|uniprotkb:YDL098C(locus name) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616232|imex:IM-25038-1 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - refseq:NP_010185.1|sgd:S000002256|mint:Q12368|dip:DIP-1647N|ensemblfungi:YDL098C(gene)|ensemblfungi:YDL098C_mRNA(transcript)|go:"GO:0000398"(mRNA splicing, via spliceosome)|go:"GO:0005681"(spliceosomal complex)|go:"GO:0046540"(U4/U6 x U5 tri-snRNP complex)|go:"GO:0046872"(metal ion binding)|interpro:IPR013087(Zinc finger, C2H2-type/integrase, DNA-binding)|interpro:IPR036236|interpro:IPR040107|rcsb pdb:5NRL|rcsb pdb:5ZWO - - crc64:4F1C0288149B91FB figure legend:Fig 3, Table 1|comment:The spiceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate B complex 0.05mM ATP was added to stall the splicing reaction.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2016/04/01 - rogid:zowIlDofJXGllNBfJuMZay/jlnE559292 - false tag:1-1|32p radiolabel:?-? - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:Q07350 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-688|uniprotkb:Q05434|ensemblfungi:YDL043C|uniprotkb:D6VRV3 psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:prp11_yeast(display_long)|uniprotkb:PRP11(gene name)|psi-mi:PRP11(display_short)|uniprotkb:PRP10(gene name synonym)|uniprotkb:RNA11(gene name synonym)|uniprotkb:YDL043C(locus name)|uniprotkb:D2711(orf name) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616232|imex:IM-25038-1 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - refseq:NP_010241.1|sgd:S000002201|ensemblfungi:YDL043C(gene)|ensemblfungi:YDL043C_mRNA(transcript)|go:"GO:0000245"(spliceosomal complex assembly)|go:"GO:0003723"(RNA binding)|go:"GO:0005686"(U2 snRNP)|go:"GO:0008270"(zinc ion binding)|go:"GO:0071004"(U2-type prespliceosome)|go:"GO:0071013"(catalytic step 2 spliceosome)|interpro:IPR003604(Zinc finger, U1-type)|interpro:IPR031781|interpro:IPR036236|rcsb pdb:4DGW|rcsb pdb:5GM6|rcsb pdb:5NRL|rcsb pdb:5ZWM|rcsb pdb:5ZWO|rcsb pdb:6G90|dip:DIP-1001N|mint:Q07350 - - crc64:D8BA3BB27F36F06F figure legend:Fig 3, Table 1|comment:The spiceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate B complex 0.05mM ATP was added to stall the splicing reaction.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2016/04/01 - rogid:xyunpB0FKbtjXifS4hyBHPmL4zs559292 - false tag:1-1|32p radiolabel:?-? - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:P19736 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-511|ensemblfungi:YDL030W|uniprotkb:D6VRW2 psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:prp9_yeast(display_long)|uniprotkb:PRP9(gene name)|psi-mi:PRP9(display_short)|uniprotkb:D2773(orf name)|uniprotkb:YDL030W(locus name) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616232|imex:IM-25038-1 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - ensemblfungi:YDL030W(gene)|ensemblfungi:YDL030W_mRNA(transcript)|go:"GO:0000395"(mRNA 5'-splice site recognition)|go:"GO:0000398"(mRNA splicing, via spliceosome)|go:"GO:0003723"(RNA binding)|go:"GO:0005681"(spliceosomal complex)|go:"GO:0008270"(zinc ion binding)|go:"GO:0071004"(U2-type prespliceosome)|interpro:IPR000690(Zinc finger, C2H2-type matrin)|interpro:IPR003604(Zinc finger, U1-type)|interpro:IPR013087(Zinc finger, C2H2-type/integrase, DNA-binding)|interpro:IPR022755|interpro:IPR024598|interpro:IPR031590|interpro:IPR031774|interpro:IPR031776|interpro:IPR036236|refseq:NP_010254.1|sgd:S000002188|rcsb pdb:4DGW|rcsb pdb:5NRL|rcsb pdb:5ZWM|rcsb pdb:5ZWO|rcsb pdb:6G90|dip:DIP-651N|mint:P19736 - - crc64:9020885E620488F3 figure legend:Fig 3, Table 1|comment:The spiceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate B complex 0.05mM ATP was added to stall the splicing reaction.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2016/04/01 - rogid:gdiY5/Azr3xdR4IiaMsyAcxJyXA559292 - false tag:1-1|32p radiolabel:?-? - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:P36048 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-243|uniprotkb:D6VX27|ensemblfungi:YKL173W psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:sn114_yeast(display_long)|uniprotkb:GIN10(gene name synonym)|uniprotkb:YKL173W(locus name)|uniprotkb:YKL637(orf name)|uniprotkb:SNU114(gene name)|psi-mi:SNU114(display_short)|uniprotkb:Growth inhibitory protein 10(gene name synonym)|uniprotkb:114 kDa U5 small nuclear ribonucleoprotein component(gene name synonym) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616232|imex:IM-25038-1 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - refseq:NP_012748.1|sgd:S000001656|mint:P36048|dip:DIP-759N|ensemblfungi:YKL173W(gene)|ensemblfungi:YKL173W_mRNA(transcript)|go:"GO:0000244"(spliceosomal tri-snRNP complex assembly)|go:"GO:0000349"(generation of catalytic spliceosome for first transesterification step)|go:"GO:0000388"("spliceosome conformational change to release U4 (or U4atac) and U1 (or U11)")|go:"GO:0000398"(mRNA splicing, via spliceosome)|go:"GO:0003924"(GTPase activity)|go:"GO:0005525"(GTP binding)|go:"GO:0005634"(nucleus)|go:"GO:0005682"(U5 snRNP)|go:"GO:0030623"(U5 snRNA binding)|go:"GO:0046540"(U4/U6 x U5 tri-snRNP complex)|go:"GO:0071007"(U2-type catalytic step 2 spliceosome)|go:"GO:1990904"(ribonucleoprotein complex)|interpro:IPR000640(Translation elongation factor EFG/EF2, C-terminal)|interpro:IPR000795(Protein synthesis factor, GTP-binding)|interpro:IPR004161(Translation elongation factor EFTu/EF1A, domain 2)|interpro:IPR005517(Translation elongation factor EFG/EF2, domain IV)|interpro:IPR009000(Translation elongation and initiation factors/Ribosomal, beta-barrel)|interpro:IPR014721(Ribosomal protein S5 domain 2-type fold)|interpro:IPR020568|interpro:IPR027417|interpro:IPR031950|interpro:IPR035647|interpro:IPR035655|interpro:IPR044121|rcsb pdb:3JCM|rcsb pdb:5GAM|rcsb pdb:5GAN|rcsb pdb:5GM6|rcsb pdb:5GMK|rcsb pdb:5LJ3|rcsb pdb:5LJ5|rcsb pdb:5LQW|rcsb pdb:5MPS|rcsb pdb:5MQ0|rcsb pdb:5NRL|rcsb pdb:5WSG|rcsb pdb:5Y88|rcsb pdb:5YLZ|rcsb pdb:5ZWM|rcsb pdb:5ZWO|rcsb pdb:6BK8|rcsb pdb:6EXN|rcsb pdb:6J6G|rcsb pdb:6J6H|rcsb pdb:6J6N|rcsb pdb:6J6Q|rcsb pdb:6TEO - - crc64:5CE30CEFF154D013 figure legend:Fig 3, Table 1|comment:The spiceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate B complex 0.05mM ATP was added to stall the splicing reaction.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2016/04/01 - rogid:eHDUPadk87+Qb525xpL1tMWb3S4559292 - false tag:1-1|32p radiolabel:?-? - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:P33203 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-701|uniprotkb:D6VXS4|ensemblfungi:YKL012W psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:prp40_yeast(display_long)|uniprotkb:YKL165(orf name)|uniprotkb:YKL012W(locus name)|uniprotkb:PRP40(gene name)|psi-mi:PRP40(display_short) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616232|imex:IM-25038-1 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - sgd:S000001495|refseq:NP_012913.3|ensemblfungi:YKL012W(gene)|ensemblfungi:YKL012W_mRNA(transcript)|go:"GO:0000398"(mRNA splicing, via spliceosome)|go:"GO:0003723"(RNA binding)|go:"GO:0005634"(nucleus)|go:"GO:0005685"(U1 snRNP)|go:"GO:0045292"(mRNA cis splicing, via spliceosome)|go:"GO:0071004"(U2-type prespliceosome)|interpro:IPR001202(WW/Rsp5/WWP)|interpro:IPR002713(FF)|interpro:IPR036020|interpro:IPR036517|interpro:IPR039726|rcsb pdb:1O6W|rcsb pdb:2B7E|rcsb pdb:2KFD|rcsb pdb:6N7P|dip:DIP-1620N|mint:P33203 - - crc64:BA1C1C91D532524C figure legend:Fig 3, Table 1|comment:The spiceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate B complex 0.05mM ATP was added to stall the splicing reaction.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2016/04/01 - rogid:y4tc4ChQBe85CA6638S4K0Bt1U8559292 - false tag:1-1|32p radiolabel:?-? - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:Q00916 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-724|uniprotkb:D6VVM3|ensemblfungi:YIL061C psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:ru17_yeast(display_long)|uniprotkb:YIL061C(locus name)|uniprotkb:SNP1(gene name)|psi-mi:SNP1(display_short)|uniprotkb:U1 small nuclear ribonucleoprotein SNP1(gene name synonym) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616232|imex:IM-25038-1 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - refseq:NP_012203.1|sgd:S000001323|dip:DIP-663N|mint:Q00916|ensemblfungi:YIL061C(gene)|ensemblfungi:YIL061C_mRNA(transcript)|go:"GO:0000243"(commitment complex)|go:"GO:0000398"(mRNA splicing, via spliceosome)|go:"GO:0003729"(mRNA binding)|go:"GO:0005685"(U1 snRNP)|go:"GO:0017069"(snRNA binding)|go:"GO:0030619"(U1 snRNA binding)|go:"GO:0071004"(U2-type prespliceosome)|interpro:IPR000504(RNA recognition motif, RNP-1)|interpro:IPR012677(Nucleotide-binding, alpha-beta plait)|interpro:IPR022023|interpro:IPR034143|interpro:IPR035979|rcsb pdb:5ZWN|rcsb pdb:6G90|rcsb pdb:6N7P|rcsb pdb:6N7R|rcsb pdb:6N7X - - crc64:7D9E47BE66FE1EB8 figure legend:Fig 3, Table 1|comment:The spiceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate B complex 0.05mM ATP was added to stall the splicing reaction.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2016/04/01 - rogid:U9mfzw792DuNErqlM1vuG+QDjzE559292 - false tag:1-1|32p radiolabel:?-? - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:P33334 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-465|uniprotkb:D3DLB4|ensemblfungi:YHR165C psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:prp8_yeast(display_long)|uniprotkb:PRP8(gene name)|psi-mi:PRP8(display_short)|uniprotkb:DBF3(gene name synonym)|uniprotkb:DNA39(gene name synonym)|uniprotkb:RNA8(gene name synonym)|uniprotkb:SLT21(gene name synonym)|uniprotkb:USA2(gene name synonym)|uniprotkb:YHR165C(locus name) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616232|imex:IM-25038-1 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - refseq:NP_012035.1|sgd:S000001208|rcsb pdb:4ILJ|rcsb pdb:5DCA|rcsb pdb:5GAM|rcsb pdb:5GAN|rcsb pdb:5GAO|rcsb pdb:5GAP|rcsb pdb:5GM6|rcsb pdb:5GMK|rcsb pdb:5LJ3|rcsb pdb:5LJ5|rcsb pdb:5LQW|rcsb pdb:5M52|rcsb pdb:5M5P|rcsb pdb:5MPS|rcsb pdb:5MQ0|rcsb pdb:5NRL|rcsb pdb:5QY1|rcsb pdb:5QY2|rcsb pdb:5QY3|rcsb pdb:5QY4|rcsb pdb:5QY5|rcsb pdb:5QY6|rcsb pdb:5QY7|rcsb pdb:5QY8|rcsb pdb:5QY9|rcsb pdb:5QYA|rcsb pdb:5QYB|rcsb pdb:5QYC|rcsb pdb:5QYD|rcsb pdb:5QYE|rcsb pdb:5QYF|rcsb pdb:5QYG|rcsb pdb:5QYH|rcsb pdb:5QYI|rcsb pdb:5QYJ|rcsb pdb:5QYK|rcsb pdb:5QYL|rcsb pdb:5QYM|rcsb pdb:5QYN|rcsb pdb:5QYO|rcsb pdb:5QYP|rcsb pdb:5QYQ|rcsb pdb:5QYR|rcsb pdb:5QYS|rcsb pdb:5QYT|rcsb pdb:5QYU|rcsb pdb:5QYV|rcsb pdb:5QYW|rcsb pdb:5QYX|rcsb pdb:5QYY|rcsb pdb:5QYZ|rcsb pdb:5QZ0|rcsb pdb:5QZ1|rcsb pdb:5QZ2|rcsb pdb:5QZ3|rcsb pdb:5QZ4|rcsb pdb:5QZ5|rcsb pdb:5QZ6|rcsb pdb:5QZ7|rcsb pdb:5QZ8|rcsb pdb:5QZ9|rcsb pdb:5QZA|rcsb pdb:5QZB|rcsb pdb:5QZC|rcsb pdb:5QZD|rcsb pdb:5QZE|rcsb pdb:5QZF|rcsb pdb:5QZG|rcsb pdb:5QZH|rcsb pdb:5QZI|rcsb pdb:5QZJ|rcsb pdb:5QZK|rcsb pdb:5QZL|rcsb pdb:5QZM|rcsb pdb:5QZN|rcsb pdb:5QZO|rcsb pdb:5QZP|rcsb pdb:5R14|rcsb pdb:5R15|rcsb pdb:5R16|rcsb pdb:5R17|rcsb pdb:5R18|rcsb pdb:5R19|rcsb pdb:5R1A|rcsb pdb:5R1B|rcsb pdb:5R1C|rcsb pdb:5R1D|rcsb pdb:5R1E|rcsb pdb:5R1F|rcsb pdb:5R1G|rcsb pdb:5R1H|rcsb pdb:5R1I|rcsb pdb:5R1J|rcsb pdb:5R1K|rcsb pdb:5R1L|rcsb pdb:5R1M|rcsb pdb:5R1N|rcsb pdb:5R1O|rcsb pdb:5R1P|rcsb pdb:5R1Q|rcsb pdb:5R1S|rcsb pdb:5WSG|rcsb pdb:5Y88|rcsb pdb:5YLZ|rcsb pdb:5ZWM|rcsb pdb:5ZWO|rcsb pdb:6BK8|rcsb pdb:6EXN|rcsb pdb:6J6G|rcsb pdb:6J6H|rcsb pdb:6J6N|rcsb pdb:6J6Q|rcsb pdb:6TEO|rcsb pdb:7B9V|rcsb pdb:5QZQ|rcsb pdb:5QZR|rcsb pdb:5QZS|rcsb pdb:5QZT|rcsb pdb:5QZU|rcsb pdb:5QZV|rcsb pdb:5QZW|rcsb pdb:5QZX|rcsb pdb:5QZY|rcsb pdb:5QZZ|rcsb pdb:5R00|rcsb pdb:5R01|rcsb pdb:5R02|rcsb pdb:5R03|rcsb pdb:5R04|rcsb pdb:5R05|rcsb pdb:5R06|rcsb pdb:5R07|rcsb pdb:5R08|rcsb pdb:5R09|rcsb pdb:5R0A|rcsb pdb:5R0B|rcsb pdb:5R0C|rcsb pdb:5R0D|rcsb pdb:5R0E|rcsb pdb:5R0F|rcsb pdb:5R0G|rcsb pdb:5R0H|rcsb pdb:5R0I|rcsb pdb:5R0J|rcsb pdb:5R0K|rcsb pdb:5R0L|rcsb pdb:5R0M|rcsb pdb:5R0N|rcsb pdb:5R0O|rcsb pdb:5R0P|rcsb pdb:5R0Q|rcsb pdb:5R0R|rcsb pdb:5R0S|rcsb pdb:5R0T|rcsb pdb:5R0U|rcsb pdb:5R0V|rcsb pdb:5R0W|rcsb pdb:5R0X|rcsb pdb:5R0Y|rcsb pdb:5R0Z|rcsb pdb:5R10|rcsb pdb:5R11|rcsb pdb:5R12|rcsb pdb:5R13|ensemblfungi:YHR165C(gene)|ensemblfungi:YHR165C_mRNA(transcript)|go:"GO:0000244"(spliceosomal tri-snRNP complex assembly)|go:"GO:0000350"(generation of catalytic spliceosome for second transesterification step)|go:"GO:0000389"(mRNA 3'-splice site recognition)|go:"GO:0000395"(mRNA 5'-splice site recognition)|go:"GO:0003729"(mRNA binding)|go:"GO:0005634"(nucleus)|go:"GO:0005682"(U5 snRNP)|go:"GO:0008237"(metallopeptidase activity)|go:"GO:0017070"(U6 snRNA binding)|go:"GO:0030619"(U1 snRNA binding)|go:"GO:0030620"(U2 snRNA binding)|go:"GO:0030623"(U5 snRNA binding)|go:"GO:0046540"(U4/U6 x U5 tri-snRNP complex)|go:"GO:0070122"(isopeptidase activity)|go:"GO:0071013"(catalytic step 2 spliceosome)|rcsb pdb:3SBT|rcsb pdb:3ZEF|rcsb pdb:4BGD|rcsb pdb:4I43|go:"GO:0097157"(pre-mRNA intronic binding)|interpro:IPR000555(Mov34/MPN/PAD-1)|interpro:IPR012337(Polynucleotidyl transferase, Ribonuclease H fold)|interpro:IPR012591(PRO8NT)|interpro:IPR012592(PROCN)|interpro:IPR012984(PRO, C-terminal)|interpro:IPR019580|interpro:IPR019581|interpro:IPR019582|interpro:IPR021983|interpro:IPR027652|interpro:IPR037518|interpro:IPR042516|interpro:IPR043172|interpro:IPR043173|rcsb pdb:2OG4|rcsb pdb:3E66|rcsb pdb:3E9O|rcsb pdb:3E9P|rcsb pdb:3JCM|rcsb pdb:3SBG|rcsb pdb:4ILG|rcsb pdb:4ILH|dip:DIP-2427N|mint:P33334 - - crc64:8F4F6F89D34D3508 figure legend:Fig 3, Table 1|comment:The spiceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate B complex 0.05mM ATP was added to stall the splicing reaction.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2016/04/01 - rogid:0JoVp7ULlbib7N5+GOPksHStSmY559292 - false tag:1-1|32p radiolabel:?-? - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:Q00539 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-11835|ensemblfungi:YHR086W|uniprotkb:D3DL37 psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:nam8_yeast(display_long)|uniprotkb:NAM8(gene name)|psi-mi:NAM8(display_short)|uniprotkb:MRE2(gene name synonym)|uniprotkb:YHR086W(locus name) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616232|imex:IM-25038-1 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - ensemblfungi:YHR086W(gene)|ensemblfungi:YHR086W_mRNA(transcript)|go:"GO:0000243"(commitment complex)|go:"GO:0000398"(mRNA splicing, via spliceosome)|go:"GO:0003729"(mRNA binding)|go:"GO:0005634"(nucleus)|go:"GO:0005737"(cytoplasm)|go:"GO:0005739"(mitochondrion)|go:"GO:0005829"(cytosol)|go:"GO:0006376"(mRNA splice site selection)|go:"GO:0048026"(positive regulation of mRNA splicing, via spliceosome)|go:"GO:0071004"(U2-type prespliceosome)|interpro:IPR000504(RNA recognition motif, RNP-1)|interpro:IPR012677(Nucleotide-binding, alpha-beta plait)|interpro:IPR035979|rcsb pdb:5ZWN|rcsb pdb:6G90|rcsb pdb:6N7P|rcsb pdb:6N7R|rcsb pdb:6N7X|go:"GO:0005685"(U1 snRNP)|refseq:NP_011954.1|sgd:S000001128|dip:DIP-2749N|mint:Q00539 - - crc64:64F198EEFB32A909 figure legend:Fig 3, Table 1|comment:The spiceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate B complex 0.05mM ATP was added to stall the splicing reaction.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2016/04/01 - rogid:WMVF9Z4Ro2MK2beb2IJy/CZ3fmA559292 - false tag:1-1|32p radiolabel:?-? - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:P32639 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-861|uniprotkb:D3DM80|ensemblfungi:YER172C psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:brr2_yeast(display_long)|uniprotkb:BRR2(gene name)|psi-mi:BRR2(display_short)|uniprotkb:RSS1(gene name synonym)|uniprotkb:SNU246(gene name synonym)|uniprotkb:YER172C(locus name)|uniprotkb:SYGP-ORF66(orf name)|uniprotkb:Protein Snu246(gene name synonym) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616232|imex:IM-25038-1 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - refseq:NP_011099.1|sgd:S000000974|dip:DIP-850N|mint:P32639|ensemblfungi:YER172C(gene)|ensemblfungi:YER172C_mRNA(transcript)|go:"GO:0000388"("spliceosome conformational change to release U4 (or U4atac) and U1 (or U11)")|go:"GO:0003723"(RNA binding)|go:"GO:0003724"(RNA helicase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005634"(nucleus)|go:"GO:0005681"(spliceosomal complex)|go:"GO:0005682"(U5 snRNP)|go:"GO:0042802"(identical protein binding)|go:"GO:0046540"(U4/U6 x U5 tri-snRNP complex)|go:"GO:0140603"(obsolete ATP hydrolysis activity)|interpro:IPR001650(DNA/RNA helicase, C-terminal)|interpro:IPR004179(Sec63)|interpro:IPR011545(DNA/RNA helicase, DEAD/DEAH box type, N-terminal)|interpro:IPR014001(DEAD-like helicase, N-terminal)|interpro:IPR014756(Immunoglobulin E-set)|interpro:IPR027417|interpro:IPR035892|interpro:IPR036388|interpro:IPR036390|interpro:IPR041094|rcsb pdb:3HIB|rcsb pdb:3IM1|rcsb pdb:3IM2|rcsb pdb:3JCM|rcsb pdb:4BGD|rcsb pdb:5DCA|rcsb pdb:5GAN|rcsb pdb:5GAO|rcsb pdb:5GAP|rcsb pdb:5GM6|rcsb pdb:5LJ5|rcsb pdb:5LQW|rcsb pdb:5M52|rcsb pdb:5M5P|rcsb pdb:5NRL|rcsb pdb:5ZWM|rcsb pdb:5ZWO|rcsb pdb:7B9V - - crc64:DFAF7E3B7168D944 figure legend:Fig 3, Table 1|comment:The spiceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate B complex 0.05mM ATP was added to stall the splicing reaction.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2016/04/01 - rogid:2bNoVkou3QatkNCtPIiSdvPdgEo559292 - false tag:1-1|32p radiolabel:?-? - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:P40089 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-10236|uniprotkb:D3DM53|ensemblfungi:YER146W psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:lsm5_yeast(display_long)|uniprotkb:LSM5(gene name)|psi-mi:LSM5(display_short)|uniprotkb:YER146W(locus name) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616232|imex:IM-25038-1 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - refseq:NP_011073.1|sgd:S000000948|ensemblfungi:YER146W(gene)|ensemblfungi:YER146W_mRNA(transcript)|go:"GO:0000398"(mRNA splicing, via spliceosome)|go:"GO:0000956"(nuclear-transcribed mRNA catabolic process)|go:"GO:0003723"(RNA binding)|go:"GO:0005681"(spliceosomal complex)|go:"GO:0005688"(U6 snRNP)|go:"GO:0005730"(nucleolus)|go:"GO:0005732"("sno(s)RNA-containing ribonucleoprotein complex")|go:"GO:0006364"(rRNA processing)|go:"GO:0008033"(tRNA processing)|go:"GO:0046540"(U4/U6 x U5 tri-snRNP complex)|go:"GO:1990726"(Lsm1-7-Pat1 complex)|interpro:IPR001163(Like-Sm ribonucleoprotein, core)|interpro:IPR010920(Like-Sm ribonucleoprotein-related, core)|interpro:IPR033871|rcsb pdb:4C8Q|rcsb pdb:3JCM|rcsb pdb:4C92|rcsb pdb:4M75|rcsb pdb:4M77|rcsb pdb:4M78|rcsb pdb:4M7A|rcsb pdb:4M7D|rcsb pdb:5GAN|rcsb pdb:5NRL|rcsb pdb:5VSU|rcsb pdb:5ZWM|rcsb pdb:5ZWO|rcsb pdb:6ASO|reactome:R-SCE-430039|dip:DIP-1417N|mint:P40089 - - crc64:84E8A1E8E2F078FE figure legend:Fig 3, Table 1|comment:The spiceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate B complex 0.05mM ATP was added to stall the splicing reaction.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2016/04/01 - rogid:+Q2uxsm6FVpx5lehw62szhlGh8U559292 - false tag:1-1|32p radiolabel:?-? - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:P40070 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-188|uniprotkb:Q07091|uniprotkb:D3DM18|ensemblfungi:YER112W psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:lsm4_yeast(display_long)|uniprotkb:LSM4(gene name)|psi-mi:LSM4(display_short)|uniprotkb:SDB23(gene name synonym)|uniprotkb:USS1(gene name synonym)|uniprotkb:YER112W(locus name) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616232|imex:IM-25038-1 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - sgd:S000000914|refseq:NP_011037.3|mint:P40070|dip:DIP-848N|ensemblfungi:YER112W(gene)|ensemblfungi:YER112W_mRNA(transcript)|go:"GO:0000387"(spliceosomal snRNP assembly)|go:"GO:0000398"(mRNA splicing, via spliceosome)|go:"GO:0000932"(P-body)|go:"GO:0000956"(nuclear-transcribed mRNA catabolic process)|go:"GO:0003723"(RNA binding)|go:"GO:0005634"(nucleus)|go:"GO:0005681"(spliceosomal complex)|go:"GO:0005688"(U6 snRNP)|go:"GO:0005730"(nucleolus)|go:"GO:0005732"("sno(s)RNA-containing ribonucleoprotein complex")|go:"GO:0005737"(cytoplasm)|go:"GO:0006364"(rRNA processing)|go:"GO:0008033"(tRNA processing)|go:"GO:0017070"(U6 snRNA binding)|go:"GO:0033962"(P-body assembly)|go:"GO:0046540"(U4/U6 x U5 tri-snRNP complex)|go:"GO:0097526"(spliceosomal tri-snRNP complex)|go:"GO:1990726"(Lsm1-7-Pat1 complex)|interpro:IPR001163(Like-Sm ribonucleoprotein, core)|interpro:IPR010920(Like-Sm ribonucleoprotein-related, core)|interpro:IPR027141|interpro:IPR034101|rcsb pdb:3JCM|rcsb pdb:4C8Q|rcsb pdb:4C92|rcsb pdb:4M75|rcsb pdb:4M77|rcsb pdb:4M78|rcsb pdb:4M7A|rcsb pdb:4M7D|rcsb pdb:5GAN|rcsb pdb:5NRL|rcsb pdb:5VSU|rcsb pdb:5ZWM|rcsb pdb:5ZWO|rcsb pdb:6ASO|reactome:R-SCE-430039 - - crc64:69CAC1F01D5BFA98 figure legend:Fig 3, Table 1|comment:The spiceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate B complex 0.05mM ATP was added to stall the splicing reaction.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2016/04/01 - rogid:Sz8HLaybwgxCRbeCESby3YThqeM559292 - false tag:1-1|32p radiolabel:?-? - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:P40018 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-432|uniprotkb:D3DLS8|ensemblfungi:YER029C psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:rsmb_yeast(display_long)|uniprotkb:SMB1(gene name)|psi-mi:SMB1(display_short)|uniprotkb:Sm protein B(gene name synonym)|uniprotkb:YER029C(locus name) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616232|imex:IM-25038-1 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - sgd:S000000831|rcsb pdb:5MPS|rcsb pdb:5MQ0|rcsb pdb:5NRL|rcsb pdb:5WSG|rcsb pdb:5Y88|rcsb pdb:5YLZ|rcsb pdb:5ZWM|rcsb pdb:5ZWN|rcsb pdb:5ZWO|rcsb pdb:6BK8|rcsb pdb:6EXN|rcsb pdb:6G90|rcsb pdb:6J6G|rcsb pdb:6J6H|rcsb pdb:6J6N|rcsb pdb:6J6Q|rcsb pdb:6N7P|rcsb pdb:6N7R|rcsb pdb:6N7X|rcsb pdb:7B9V|ensemblfungi:YER029C(gene)|ensemblfungi:YER029C_mRNA(transcript)|go:"GO:0000398"(mRNA splicing, via spliceosome)|go:"GO:0003723"(RNA binding)|go:"GO:0005682"(U5 snRNP)|go:"GO:0005685"(U1 snRNP)|go:"GO:0005686"(U2 snRNP)|go:"GO:0005687"(U4 snRNP)|go:"GO:0005737"(cytoplasm)|go:"GO:0046540"(U4/U6 x U5 tri-snRNP complex)|go:"GO:0071004"(U2-type prespliceosome)|go:"GO:0071013"(catalytic step 2 spliceosome)|interpro:IPR001163(Like-Sm ribonucleoprotein, core)|interpro:IPR010920(Like-Sm ribonucleoprotein-related, core)|rcsb pdb:3JCM|rcsb pdb:5GAM|rcsb pdb:5GAN|rcsb pdb:5GAO|rcsb pdb:5GM6|refseq:NP_010946.3|rcsb pdb:5GMK|rcsb pdb:5LJ3|rcsb pdb:5LJ5|rcsb pdb:5LQW|dip:DIP-843N|mint:P40018 - - crc64:0A1078D5AA241430 figure legend:Fig 3, Table 1|comment:The spiceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate B complex 0.05mM ATP was added to stall the splicing reaction.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2016/04/01 - rogid:b62+dBOdvQgeE0oup4kbXbviS1U559292 - false tag:1-1|32p radiolabel:?-? - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:P21372 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-13824|ensemblfungi:YBR237W|uniprotkb:D6VQN3 psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:prp5_yeast(display_long)|uniprotkb:PRP5(gene name)|psi-mi:PRP5(display_short)|uniprotkb:RNA5(gene name synonym)|uniprotkb:YBR237W(locus name)|uniprotkb:YBR1603(orf name) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616232|imex:IM-25038-1 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - refseq:NP_009796.1|sgd:S000000441|ensemblfungi:YBR237W(gene)|ensemblfungi:YBR237W_mRNA(transcript)|go:"GO:0000348"(mRNA branch site recognition)|go:"GO:0000398"(mRNA splicing, via spliceosome)|go:"GO:0003676"(nucleic acid binding)|go:"GO:0003724"(RNA helicase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005634"(nucleus)|go:"GO:0008186"(ATP-dependent activity, acting on RNA)|go:"GO:0140603"(obsolete ATP hydrolysis activity)|interpro:IPR000629(RNA helicase, ATP-dependent, DEAD-box, conserved site)|interpro:IPR001650(DNA/RNA helicase, C-terminal)|interpro:IPR011545(DNA/RNA helicase, DEAD/DEAH box type, N-terminal)|interpro:IPR014001(DEAD-like helicase, N-terminal)|interpro:IPR014014(RNA helicase, DEAD-box type, Q motif)|interpro:IPR027417|rcsb pdb:4LJY|rcsb pdb:4LK2|mint:P21372|dip:DIP-88N - - - figure legend:Fig 3, Table 1|comment:The spiceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate B complex 0.05mM ATP was added to stall the splicing reaction.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2016/04/01 - rogid:0aE2/P83FZO9bSFTxeCB7o5NY2w559292 - false tag:1-1|32p radiolabel:?-? - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:P38302 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-20921|uniprotkb:D6VQI3|ensemblfungi:YBR188C psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:ntc20_yeast(display_long)|uniprotkb:YBR188C(locus name)|uniprotkb:YBR1316(orf name)|uniprotkb:NTC20(gene name)|psi-mi:NTC20(display_short)|uniprotkb:SNT384(gene name synonym)|uniprotkb:PRP19-associated complex protein 20(gene name synonym)|uniprotkb:Synergistic to PRP19 mutation protein 384(gene name synonym) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616232|imex:IM-25038-1 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - refseq:NP_009747.3|sgd:S000000392|mint:P38302|ensemblfungi:YBR188C(gene)|ensemblfungi:YBR188C_mRNA(transcript)|go:"GO:0000398"(mRNA splicing, via spliceosome)|go:"GO:0000974"(Prp19 complex)|go:"GO:0071006"(U2-type catalytic step 1 spliceosome)|dip:DIP-1683N - - crc64:15C5234B25213223 figure legend:Fig 3, Table 1|comment:The spiceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate B complex 0.05mM ATP was added to stall the splicing reaction.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2016/04/01 - rogid:Ucc4R0lhhhAQfrsXvs+BtCnuJQg559292 - false tag:1-1|32p radiolabel:?-? - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:P38282 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-443|uniprotkb:D6VQE7|ensemblfungi:YBR152W psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:sp381_yeast(display_long)|uniprotkb:YBR152W(locus name)|uniprotkb:YBR1202(orf name)|uniprotkb:SPP381(gene name)|psi-mi:SPP381(display_short)|uniprotkb:Suppressor of PRP38-1 mutation(gene name synonym) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616232|imex:IM-25038-1 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - sgd:S000000356|ensemblfungi:YBR152W(gene)|ensemblfungi:YBR152W_mRNA(transcript)|go:"GO:0000349"(generation of catalytic spliceosome for first transesterification step)|go:"GO:0000398"(mRNA splicing, via spliceosome)|go:"GO:0003723"(RNA binding)|go:"GO:0005634"(nucleus)|go:"GO:0005681"(spliceosomal complex)|go:"GO:0005829"(cytosol)|go:"GO:0046540"(U4/U6 x U5 tri-snRNP complex)|rcsb pdb:5NRL|rcsb pdb:5ZWO|refseq:NP_009710.3|dip:DIP-2461N|mint:P38282 - - crc64:B65D0F00DE860A83 figure legend:Fig 3, Table 1|comment:The spiceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate B complex 0.05mM ATP was added to stall the splicing reaction.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2016/04/01 - rogid:7cMApDnLJrd3RVfvA9nJw8baEAA559292 - false tag:1-1|32p radiolabel:?-? - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:P32605 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-680|uniprotkb:D6VQB7|ensemblfungi:YBR119W psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:ru1a_yeast(display_long)|uniprotkb:MUD1(gene name)|psi-mi:MUD1(display_short)|uniprotkb:YBR119W(locus name)|uniprotkb:YBR0915(orf name)|uniprotkb:Mutant U1 die protein 1(gene name synonym) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616232|imex:IM-25038-1 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - refseq:NP_009677.1|sgd:S000000323|dip:DIP-223N|mint:P32605|ensemblfungi:YBR119W(gene)|ensemblfungi:YBR119W_mRNA(transcript)|go:"GO:0000398"(mRNA splicing, via spliceosome)|go:"GO:0005685"(U1 snRNP)|go:"GO:0030619"(U1 snRNA binding)|go:"GO:0071004"(U2-type prespliceosome)|interpro:IPR000504(RNA recognition motif, RNP-1)|interpro:IPR012677(Nucleotide-binding, alpha-beta plait)|interpro:IPR035979|rcsb pdb:5ZWN|rcsb pdb:6G90|rcsb pdb:6N7P|rcsb pdb:6N7R|rcsb pdb:6N7X - - - figure legend:Fig 3, Table 1|comment:The spiceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate B complex 0.05mM ATP was added to stall the splicing reaction.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2016/04/01 - rogid:Kdy7HrGf/niY7yMw1XnEnvtoyec559292 - false tag:1-1|32p radiolabel:?-? - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:P19735 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-227|uniprotkb:D6VQ54|ensemblfungi:YBR055C psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:prp6_yeast(display_long)|uniprotkb:PRP6(gene name)|psi-mi:PRP6(display_short)|uniprotkb:RNA6(gene name synonym)|uniprotkb:YBR055C(locus name)|uniprotkb:YBR0508(orf name) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616232|imex:IM-25038-1 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - refseq:NP_009611.1|sgd:S000000259|mint:P19735|dip:DIP-1290N|ensemblfungi:YBR055C(gene)|ensemblfungi:YBR055C_mRNA(transcript)|go:"GO:0000244"(spliceosomal tri-snRNP complex assembly)|go:"GO:0000398"(mRNA splicing, via spliceosome)|go:"GO:0046540"(U4/U6 x U5 tri-snRNP complex)|go:"GO:0071001"(U4/U6 snRNP)|go:"GO:0071013"(catalytic step 2 spliceosome)|interpro:IPR003107(RNA-processing protein, HAT helix)|interpro:IPR010491(PRP1 splicing factor, N-terminal)|interpro:IPR011990(Tetratricopeptide-like helical)|interpro:IPR019734|interpro:IPR027108|rcsb pdb:3JCM|rcsb pdb:5GAN|rcsb pdb:5GAP|rcsb pdb:5NRL|rcsb pdb:5ZWM|rcsb pdb:5ZWO - - crc64:3E87F8AF63EAAC41 figure legend:Fig 3, Table 1|comment:The spiceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate B complex 0.05mM ATP was added to stall the splicing reaction.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2016/04/01 - rogid:v3xgQmlk5z/P4uu09sipcLZmlpE559292 - false tag:1-1|32p radiolabel:?-? - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:P38203 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-180|ensemblfungi:YBL026W|uniprotkb:D6VPX4 psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:lsm2_yeast(display_long)|uniprotkb:LSM2(gene name)|psi-mi:LSM2(display_short)|uniprotkb:Small nuclear ribonucleoprotein D homolog SNP3(gene name synonym)|uniprotkb:SMX5(gene name synonym)|uniprotkb:SNP3(gene name synonym)|uniprotkb:YBL0425(orf name)|uniprotkb:YBL026W(locus name) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616232|imex:IM-25038-1 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - refseq:NP_009527.1|sgd:S000000122|go:"GO:0005688"(U6 snRNP)|go:"GO:0005730"(nucleolus)|go:"GO:0005732"("sno(s)RNA-containing ribonucleoprotein complex")|go:"GO:0005737"(cytoplasm)|go:"GO:0006364"(rRNA processing)|go:"GO:0008033"(tRNA processing)|go:"GO:0046540"(U4/U6 x U5 tri-snRNP complex)|go:"GO:0071011"(precatalytic spliceosome)|go:"GO:0071013"(catalytic step 2 spliceosome)|go:"GO:1990726"(Lsm1-7-Pat1 complex)|interpro:IPR001163(Like-Sm ribonucleoprotein, core)|interpro:IPR010920(Like-Sm ribonucleoprotein-related, core)|interpro:IPR016654(U6 snRNA-associated Sm-like protein LSm2)|rcsb pdb:3JCM|rcsb pdb:4C8Q|rcsb pdb:4C92|rcsb pdb:4M75|rcsb pdb:4M77|ensemblfungi:YBL026W(gene)|ensemblfungi:YBL026W_mRNA(transcript)|go:"GO:0000398"(mRNA splicing, via spliceosome)|go:"GO:0000932"(P-body)|go:"GO:0003723"(RNA binding)|go:"GO:0005634"(nucleus)|mint:P38203|dip:DIP-794N|rcsb pdb:4M78|rcsb pdb:4M7A|rcsb pdb:4M7D|rcsb pdb:4N0A|rcsb pdb:5GAN|rcsb pdb:5NRL|rcsb pdb:5VSU|rcsb pdb:5ZWM|rcsb pdb:5ZWO|rcsb pdb:6ASO|reactome:R-SCE-430039 - - crc64:C23856268CEE92E2 figure legend:Fig 3, Table 1|comment:The spiceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate B complex 0.05mM ATP was added to stall the splicing reaction.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2016/04/01 - rogid:3E5c7Mzg433awFPmamamsKjtJmY559292 - false tag:1-1|32p radiolabel:?-? - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:P28004 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-20640|uniprotkb:D6VPI6|ensemblfungi:YAL032C psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:prp45_yeast(display_long)|uniprotkb:YAL032C(locus name)|uniprotkb:PRP45(gene name)|psi-mi:PRP45(display_short)|uniprotkb:FUN20(gene name synonym) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616232|imex:IM-25038-1 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - refseq:NP_009370.1|sgd:S000000030|ensemblfungi:YAL032C(gene)|ensemblfungi:YAL032C_mRNA(transcript)|go:"GO:0000350"(generation of catalytic spliceosome for second transesterification step)|go:"GO:0005634"(nucleus)|go:"GO:0071006"(U2-type catalytic step 1 spliceosome)|go:"GO:0071007"(U2-type catalytic step 2 spliceosome)|go:"GO:0071014"(post-mRNA release spliceosomal complex)|interpro:IPR004015(SKIP/SNW)|interpro:IPR017862|rcsb pdb:5GM6|rcsb pdb:5GMK|rcsb pdb:5LJ3|rcsb pdb:5LJ5|rcsb pdb:5LQW|rcsb pdb:5MPS|rcsb pdb:5MQ0|rcsb pdb:5WSG|rcsb pdb:5Y88|rcsb pdb:5YLZ|rcsb pdb:6BK8|rcsb pdb:6EXN|rcsb pdb:6J6G|rcsb pdb:6J6H|rcsb pdb:6J6N|rcsb pdb:6J6Q|dip:DIP-2774N|mint:P28004 - - crc64:FE5A724186D7FE84 figure legend:Fig 3, Table 1|comment:The spiceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate B complex 0.05mM ATP was added to stall the splicing reaction.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2016/04/01 - rogid:qoHF1UsO3k+JOTOfigSuy2P84/k559292 - false tag:1-1|32p radiolabel:?-? - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 intact:EBI-10824938 ensembl:YFL039C.1|sgd:YFL039C rnacentral:URS00006184A9_559292 psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:snru6_yeast(display_short)|psi-mi:EBI-10824938(display_long) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616232|imex:IM-25038-1 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0607"(small nuclear rna) - - - - - figure legend:Fig 3, Table 1|comment:The spiceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate B complex 0.05mM ATP was added to stall the splicing reaction.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2016/04/01 - - - false tag:1-1|32p radiolabel:?-? - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0929"(northern blot) intact:EBI-11413715 intact:EBI-10860871 ensembl:YFL039C.1|sgd:YFL039C rnacentral:URS000023C730_559292 psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:lsr1_snr20_u2 yeast(display_short)|psi-mi:EBI-10860871(display_long) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616232|imex:IM-25038-1 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0607"(small nuclear rna) - - - - - figure legend:Fig 3, Table 1|comment:The spiceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate B complex 0.05mM ATP was added to stall the splicing reaction.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2016/04/01 - - - false tag:1-1|32p radiolabel:?-? - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0929"(northern blot) intact:EBI-11413715 intact:EBI-10825220 ensembl:YFL039C.1|sgd:YFL039C rnacentral:URS0000514318_559292 psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:snr7s_u5_yeast(display_short)|psi-mi:EBI-10825220(display_long) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616232|imex:IM-25038-1 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0607"(small nuclear rna) - - - - - figure legend:Fig 3, Table 1|comment:The spiceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate B complex 0.05mM ATP was added to stall the splicing reaction.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2016/04/01 - - - false tag:1-1|32p radiolabel:?-? - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0929"(northern blot) intact:EBI-11413715 intact:EBI-10054797 ensembl:YFL039C.1|sgd:YFL039C rnacentral:URS00001143F5_559292 psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:snr14_u4_yeast(display_short)|psi-mi:EBI-10054797(display_long) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616232|imex:IM-25038-1 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0607"(small nuclear rna) - - - - - figure legend:Fig 3, Table 1|comment:The spiceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate B complex 0.05mM ATP was added to stall the splicing reaction.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2016/04/01 - - - false tag:1-1|32p radiolabel:?-? - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 intact:EBI-10054789 ensembl:YFL039C.1|sgd:YFL039C rnacentral:URS000012D749_559292 psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:snr19_u1_yeast(display_short)|psi-mi:EBI-10054789(display_long) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616232|imex:IM-25038-1 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0607"(small nuclear rna) - - - - - figure legend:Fig 3, Table 1|comment:The spiceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate B complex 0.05mM ATP was added to stall the splicing reaction.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2016/04/01 - - - false tag:1-1|32p radiolabel:?-? - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:P0C074 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-970846|uniprotkb:D6W143|ensemblfungi:YNL138W-A psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:ysf3_yeast(display_long)|uniprotkb:YSF3(gene name)|psi-mi:YSF3(display_short)|uniprotkb:RCP10(gene name synonym)|uniprotkb:YNL138W-A(locus name)|uniprotkb:Splicing factor 3b subunit(gene name synonym) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616300|imex:IM-25038-2 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - go:"GO:0000398"(mRNA splicing, via spliceosome)|go:"GO:0005686"(U2 snRNP)|go:"GO:0071011"(precatalytic spliceosome)|ensemblfungi:YNL138W-A(gene)|ensemblfungi:YNL138W-A_mRNA(transcript)|go:"GO:0000245"(spliceosomal complex assembly)|refseq:NP_878153.1|sgd:S000028509|interpro:IPR009846(Splicing factor 3B subunit 5/RDS3 complex subunit 10)|rcsb pdb:7DCO|rcsb pdb:5GM6|rcsb pdb:5LQW|rcsb pdb:5ZWM|rcsb pdb:5ZWO|rcsb pdb:6G90|rcsb pdb:5NRL - - - figure legend:Table 1, Suppl. Fig 3B|comment:The spliceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate B act complex 2mM ATP was added and a deleted mutant of actin mRNA was used to stall the splicing reaction.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2016/04/01 - rogid:+ifBAgC9wh9hksXMFWXrcwu8eOU559292 - false mutation:284-1437|tag:1-1|32p radiolabel:?-? - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 intact:EBI-10825220 ensembl:YFL039C.1|sgd:YFL039C rnacentral:URS0000514318_559292 psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:snr7s_u5_yeast(display_short)|psi-mi:EBI-10825220(display_long) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616300|imex:IM-25038-2 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0607"(small nuclear rna) - - - - - figure legend:Table 1, Suppl. Fig 3B|comment:The spliceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate B act complex 2mM ATP was added and a deleted mutant of actin mRNA was used to stall the splicing reaction.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2016/04/01 - - - false mutation:284-1437|tag:1-1|32p radiolabel:?-? - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0929"(northern blot) intact:EBI-11413715 intact:EBI-10824938 ensembl:YFL039C.1|sgd:YFL039C rnacentral:URS00006184A9_559292 psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:snru6_yeast(display_short)|psi-mi:EBI-10824938(display_long) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616300|imex:IM-25038-2 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0607"(small nuclear rna) - - - - - figure legend:Table 1, Suppl. Fig 3B|comment:The spliceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate B act complex 2mM ATP was added and a deleted mutant of actin mRNA was used to stall the splicing reaction.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2016/04/01 - - - false mutation:284-1437|tag:1-1|32p radiolabel:?-? - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0929"(northern blot) intact:EBI-11413715 intact:EBI-10860871 ensembl:YFL039C.1|sgd:YFL039C rnacentral:URS000023C730_559292 psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:lsr1_snr20_u2 yeast(display_short)|psi-mi:EBI-10860871(display_long) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616300|imex:IM-25038-2 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0607"(small nuclear rna) - - - - - figure legend:Table 1, Suppl. Fig 3B|comment:The spliceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate B act complex 2mM ATP was added and a deleted mutant of actin mRNA was used to stall the splicing reaction.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2016/04/01 - - - false mutation:284-1437|tag:1-1|32p radiolabel:?-? - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0929"(northern blot) intact:EBI-11413715 uniprotkb:Q04693 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-519|ensemblfungi:YML049C|uniprotkb:D6VZC6 psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:rse1_yeast(display_long)|uniprotkb:RSE1(gene name)|psi-mi:RSE1(display_short)|uniprotkb:YML049C(locus name)|uniprotkb:YM9827.03C(orf name)|uniprotkb:SAP130(gene name synonym)|uniprotkb:RNA splicing and ER to Golgi transport factor 1(gene name synonym)|uniprotkb:Spliceosome-associated protein 130(gene name synonym) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616300|imex:IM-25038-2 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - refseq:NP_013663.1|sgd:S000004513|ensemblfungi:YML049C(gene)|ensemblfungi:YML049C_mRNA(transcript)|go:"GO:0000245"(spliceosomal complex assembly)|go:"GO:0000398"(mRNA splicing, via spliceosome)|go:"GO:0005634"(nucleus)|go:"GO:0005686"(U2 snRNP)|go:"GO:0030620"(U2 snRNA binding)|go:"GO:0071004"(U2-type prespliceosome)|interpro:IPR004871(Cleavage and polyadenylation specificity factor, A subunit, C-terminal)|interpro:IPR015943(WD40/YVTN repeat-like)|interpro:IPR018846|rcsb pdb:5GM6|rcsb pdb:5LQW|rcsb pdb:5NRL|rcsb pdb:5ZWM|rcsb pdb:5ZWO|rcsb pdb:6G90|dip:DIP-856N|mint:Q04693 - - crc64:4D92837F5C267D67 figure legend:Table 1, Suppl. Fig 3B|comment:The spliceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate B act complex 2mM ATP was added and a deleted mutant of actin mRNA was used to stall the splicing reaction.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2016/04/01 - rogid:L2J86R3nXgMd8CE/6Yd2jxA/sx4559292 - false mutation:284-1437|tag:1-1|32p radiolabel:?-? - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:P54999 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-770|uniprotkb:D6W4I2|ensemblfungi:YPR182W psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:ruxf_yeast(display_long)|uniprotkb:SMX3(gene name)|psi-mi:SMX3(display_short)|uniprotkb:YPR182W(locus name)|uniprotkb:P9705.4(orf name)|uniprotkb:Sm protein F(gene name synonym) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616300|imex:IM-25038-2 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - refseq:NP_015508.1|sgd:S000006386|dip:DIP-1634N|mint:P54999|ensemblfungi:YPR182W(gene)|ensemblfungi:YPR182W_mRNA(transcript)|go:"GO:0000387"(spliceosomal snRNP assembly)|go:"GO:0000398"(mRNA splicing, via spliceosome)|go:"GO:0003723"(RNA binding)|go:"GO:0005682"(U5 snRNP)|go:"GO:0005685"(U1 snRNP)|go:"GO:0005686"(U2 snRNP)|go:"GO:0008266"("poly(U) RNA binding")|go:"GO:0046540"(U4/U6 x U5 tri-snRNP complex)|go:"GO:0071013"(catalytic step 2 spliceosome)|go:"GO:1990935"(splicing factor binding)|interpro:IPR001163(Like-Sm ribonucleoprotein, core)|interpro:IPR010920(Like-Sm ribonucleoprotein-related, core)|interpro:IPR016487(Small nuclear ribonucleoprotein SmF)|interpro:IPR034100|rcsb pdb:1N9R|rcsb pdb:1N9S|rcsb pdb:3JCM|rcsb pdb:5GAM|rcsb pdb:5GAN|rcsb pdb:5GAO|rcsb pdb:5GM6|rcsb pdb:5GMK|rcsb pdb:5LJ3|rcsb pdb:5LJ5|rcsb pdb:5LQW|rcsb pdb:5MPS|rcsb pdb:5MQ0|rcsb pdb:5NRL|rcsb pdb:5WSG|rcsb pdb:5Y88|rcsb pdb:5YLZ|rcsb pdb:5ZWM|rcsb pdb:5ZWN|rcsb pdb:5ZWO|rcsb pdb:6BK8|rcsb pdb:6EXN|rcsb pdb:6G90|rcsb pdb:6J6G|rcsb pdb:6J6H|rcsb pdb:6J6N|rcsb pdb:6J6Q|rcsb pdb:6N7P|rcsb pdb:6N7R|rcsb pdb:6N7X|rcsb pdb:7B9V - - crc64:1640D3237597B2D8 figure legend:Table 1, Suppl. Fig 3B|comment:The spliceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate B act complex 2mM ATP was added and a deleted mutant of actin mRNA was used to stall the splicing reaction.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2016/04/01 - rogid:iyBbVNf2fgOIqhnOg9Etn3P2r3Q559292 - false mutation:284-1437|tag:1-1|32p radiolabel:?-? - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:Q06091 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-818|uniprotkb:D6W499|ensemblfungi:YPR101W psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:sn309_yeast(display_long)|uniprotkb:YPR101W(locus name)|uniprotkb:SNT309(gene name)|psi-mi:SNT309(display_short)|uniprotkb:NTC25(gene name synonym)|uniprotkb:Synergistic to PRP19 mutation protein 309(gene name synonym)|uniprotkb:PRP19-associated complex protein 25(gene name synonym) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616300|imex:IM-25038-2 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - sgd:S000006305|refseq:NP_015426.1|dip:DIP-2864N|mint:Q06091|ensemblfungi:YPR101W(gene)|ensemblfungi:YPR101W_mRNA(transcript)|go:"GO:0000398"(mRNA splicing, via spliceosome)|go:"GO:0000974"(Prp19 complex)|go:"GO:0071006"(U2-type catalytic step 1 spliceosome)|rcsb pdb:5GM6|rcsb pdb:5GMK|rcsb pdb:5LJ5|rcsb pdb:5MQ0|rcsb pdb:5WSG|rcsb pdb:5Y88|rcsb pdb:5YLZ|rcsb pdb:6BK8|rcsb pdb:6EXN|rcsb pdb:6J6G|rcsb pdb:6J6H|rcsb pdb:6J6N|rcsb pdb:6J6Q - - crc64:B781CA65BC9A1918 figure legend:Table 1, Suppl. Fig 3B|comment:The spliceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate B act complex 2mM ATP was added and a deleted mutant of actin mRNA was used to stall the splicing reaction.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2016/04/01 - rogid:ELyyYlQDmNRzWKQx/63xvHuAG8M559292 - false mutation:284-1437|tag:1-1|32p radiolabel:?-? - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:Q06835 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-29456|uniprotkb:D6W493|ensemblfungi:YPR094W psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:rds3_yeast(display_long)|uniprotkb:Regulator of drug sensitivity 3(gene name synonym)|uniprotkb:RDS3(gene name)|psi-mi:RDS3(display_short)|uniprotkb:YPR094W(locus name)|uniprotkb:P9513.2(orf name) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616300|imex:IM-25038-2 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - refseq:NP_015419.1|sgd:S000006298|dip:DIP-3822N|ensemblfungi:YPR094W(gene)|ensemblfungi:YPR094W_mRNA(transcript)|go:"GO:0000245"(spliceosomal complex assembly)|go:"GO:0000398"(mRNA splicing, via spliceosome)|go:"GO:0005686"(U2 snRNP)|go:"GO:0009410"(response to xenobiotic stimulus)|go:"GO:0071011"(precatalytic spliceosome)|interpro:IPR005345(PHF5-like)|rcsb pdb:2K0A|rcsb pdb:5GM6|rcsb pdb:5LQW|rcsb pdb:5NRL|rcsb pdb:5ZWM|rcsb pdb:5ZWO|rcsb pdb:6G90 - - - figure legend:Table 1, Suppl. Fig 3B|comment:The spliceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate B act complex 2mM ATP was added and a deleted mutant of actin mRNA was used to stall the splicing reaction.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2016/04/01 - rogid:2nInG/eG4Kui0I5TGy2Wh2fxy8o559292 - false mutation:284-1437|tag:1-1|32p radiolabel:?-? - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:Q08963 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-594|uniprotkb:D6W3F7|ensemblfungi:YPL213W psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:ru2a_yeast(display_long)|uniprotkb:YPL213W(locus name)|uniprotkb:LEA1(gene name)|psi-mi:LEA1(display_short)|uniprotkb:Looks exceptionally like U2A protein 1(gene name synonym) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616300|imex:IM-25038-2 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - refseq:NP_015111.1|sgd:S000006134|dip:DIP-2399N|mint:Q08963|ensemblfungi:YPL213W(gene)|ensemblfungi:YPL213W_mRNA(transcript)|go:"GO:0000398"(mRNA splicing, via spliceosome)|go:"GO:0005686"(U2 snRNP)|go:"GO:0005737"(cytoplasm)|go:"GO:0030620"(U2 snRNA binding)|go:"GO:0071004"(U2-type prespliceosome)|interpro:IPR001611(Leucine-rich repeat)|interpro:IPR003603(U2A'/phosphoprotein 32 family A, C-terminal)|interpro:IPR032675|interpro:IPR044640|rcsb pdb:5GMK|rcsb pdb:5LJ3|rcsb pdb:5LJ5|rcsb pdb:5MQ0|rcsb pdb:5NRL|rcsb pdb:5WSG|rcsb pdb:5Y88|rcsb pdb:5YLZ|rcsb pdb:5ZWM|rcsb pdb:5ZWO|rcsb pdb:6BK8|rcsb pdb:6EXN|rcsb pdb:6G90|rcsb pdb:6J6G|rcsb pdb:6J6H|rcsb pdb:6J6N|rcsb pdb:6J6Q - - - figure legend:Table 1, Suppl. Fig 3B|comment:The spliceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate B act complex 2mM ATP was added and a deleted mutant of actin mRNA was used to stall the splicing reaction.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2016/04/01 - rogid:PlKwD+gRYbhUBE4GcxTaP0wt8zM559292 - false mutation:284-1437|tag:1-1|32p radiolabel:?-? - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:Q12417 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-710|uniprotkb:D6W3L8|ensemblfungi:YPL151C psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:prp46_yeast(display_long)|uniprotkb:CWC1(gene name synonym)|uniprotkb:PRP nineteen-associated complex protein 50(gene name synonym)|uniprotkb:PRP19-associated complex protein 50(gene name synonym)|uniprotkb:P2594(orf name)|uniprotkb:YPL151C(locus name)|uniprotkb:PRP46(gene name)|psi-mi:PRP46(display_short)|uniprotkb:NTC50(gene name synonym)|uniprotkb:Pre-mRNA-processing protein 46(gene name synonym)|uniprotkb:Complexed with CEF1 protein 1(gene name synonym) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616300|imex:IM-25038-2 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - refseq:NP_015174.1|sgd:S000006072|ensemblfungi:YPL151C(gene)|ensemblfungi:YPL151C_mRNA(transcript)|go:"GO:0000398"(mRNA splicing, via spliceosome)|go:"GO:0000974"(Prp19 complex)|go:"GO:0005681"(spliceosomal complex)|go:"GO:0005737"(cytoplasm)|go:"GO:0071013"(catalytic step 2 spliceosome)|go:"GO:0080008"(Cul4-RING E3 ubiquitin ligase complex)|interpro:IPR001680(WD40 repeat)|interpro:IPR015943(WD40/YVTN repeat-like)|interpro:IPR019775|interpro:IPR020472|interpro:IPR036322|dip:DIP-3994N|mint:Q12417|rcsb pdb:5GM6|rcsb pdb:5GMK|rcsb pdb:5LJ3|rcsb pdb:5LJ5|rcsb pdb:5LQW|rcsb pdb:5MPS|rcsb pdb:5MQ0|rcsb pdb:5WSG|rcsb pdb:5Y88|rcsb pdb:5YLZ|rcsb pdb:6BK8|rcsb pdb:6EXN|rcsb pdb:6J6G|rcsb pdb:6J6H|rcsb pdb:6J6N|rcsb pdb:6J6Q - - crc64:0E888F566A5414BD figure legend:Table 1, Suppl. Fig 3B|comment:The spliceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate B act complex 2mM ATP was added and a deleted mutant of actin mRNA was used to stall the splicing reaction.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2016/04/01 - rogid:016ynVER9V3d44NwkqRG7TIvf9w559292 - false mutation:284-1437|tag:1-1|32p radiolabel:?-? - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:Q02770 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-35484|uniprotkb:D6W3V1|ensemblfungi:YPL064C psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:cwc27_yeast(display_long)|uniprotkb:CWC27(gene name)|psi-mi:CWC27(display_short)|uniprotkb:Complexed with CEF1 protein 27(gene name synonym)|uniprotkb:Rotamase CWC27(gene name synonym)|uniprotkb:CYP7(gene name synonym)|uniprotkb:YPL064C(locus name) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616300|imex:IM-25038-2 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - refseq:NP_015261.1|sgd:S000005985|dip:DIP-2778N|ensemblfungi:YPL064C(gene)|ensemblfungi:YPL064C_mRNA(transcript)|go:"GO:0000413"(protein peptidyl-prolyl isomerization)|go:"GO:0003755"(peptidyl-prolyl cis-trans isomerase activity)|go:"GO:0005684"(U2-type spliceosomal complex)|go:"GO:0005737"(cytoplasm)|go:"GO:0006397"(mRNA processing)|go:"GO:0008380"(RNA splicing)|interpro:IPR002130(Peptidyl-prolyl cis-trans isomerase, cyclophilin-type)|interpro:IPR029000|interpro:IPR044666|rcsb pdb:5GM6 - - - figure legend:Table 1, Suppl. Fig 3B|comment:The spliceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate B act complex 2mM ATP was added and a deleted mutant of actin mRNA was used to stall the splicing reaction.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2016/04/01 - rogid:evoBtq7Y4Qzpk0toZEmGq1ECihM559292 - false mutation:284-1437|tag:1-1|32p radiolabel:?-? - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:Q99181 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-8579|uniprotkb:D6W317|ensemblfungi:YOR319W psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:hsh49_yeast(display_long)|uniprotkb:O6142(orf name)|uniprotkb:YOR319W(locus name)|uniprotkb:HSH49(gene name)|psi-mi:HSH49(display_short) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616300|imex:IM-25038-2 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - refseq:NP_014964.1|sgd:S000005846|mint:Q99181|dip:DIP-930N|ensemblfungi:YOR319W(gene)|ensemblfungi:YOR319W_mRNA(transcript)|go:"GO:0000398"(mRNA splicing, via spliceosome)|go:"GO:0003723"(RNA binding)|go:"GO:0003730"(mRNA 3'-UTR binding)|go:"GO:0005634"(nucleus)|go:"GO:0005686"(U2 snRNP)|go:"GO:0005829"(cytosol)|go:"GO:0008143"("poly(A) binding")|go:"GO:0008266"("poly(U) RNA binding")|go:"GO:0071004"(U2-type prespliceosome)|go:"GO:1990904"(ribonucleoprotein complex)|interpro:IPR000504(RNA recognition motif, RNP-1)|interpro:IPR012677(Nucleotide-binding, alpha-beta plait)|interpro:IPR034158|interpro:IPR035979|rcsb pdb:5GM6|rcsb pdb:5LSB|rcsb pdb:5LSL|rcsb pdb:5NRL|rcsb pdb:5ZWM|rcsb pdb:5ZWO|rcsb pdb:6G90 - - crc64:F6FC44083094B176 figure legend:Table 1, Suppl. Fig 3B|comment:The spliceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate B act complex 2mM ATP was added and a deleted mutant of actin mRNA was used to stall the splicing reaction.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2016/04/01 - rogid:LWcxvibHcl0Oyi1OidqUtMnOLP8559292 - false mutation:284-1437|tag:1-1|32p radiolabel:?-? - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:Q12330 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-16359|uniprotkb:D6W2L6|ensemblfungi:YOR159C psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:ruxe_yeast(display_long)|uniprotkb:SME1(gene name)|psi-mi:SME1(display_short)|uniprotkb:YOR159C(locus name)|uniprotkb:Sm protein E(gene name synonym) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616300|imex:IM-25038-2 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - ensemblfungi:YOR159C(gene)|ensemblfungi:YOR159C_mRNA(transcript)|go:"GO:0000387"(spliceosomal snRNP assembly)|go:"GO:0000398"(mRNA splicing, via spliceosome)|go:"GO:0003723"(RNA binding)|go:"GO:0005682"(U5 snRNP)|go:"GO:0005685"(U1 snRNP)|go:"GO:0005686"(U2 snRNP)|go:"GO:0005687"(U4 snRNP)|go:"GO:0005737"(cytoplasm)|go:"GO:0046540"(U4/U6 x U5 tri-snRNP complex)|go:"GO:0071004"(U2-type prespliceosome)|go:"GO:0071011"(precatalytic spliceosome)|go:"GO:1990935"(splicing factor binding)|interpro:IPR001163(Like-Sm ribonucleoprotein, core)|interpro:IPR010920(Like-Sm ribonucleoprotein-related, core)|interpro:IPR027078|rcsb pdb:3JCM|rcsb pdb:5GAM|rcsb pdb:5GAN|rcsb pdb:5GAO|rcsb pdb:5GM6|rcsb pdb:5GMK|sgd:S000005685|rcsb pdb:5LJ3|rcsb pdb:5LJ5|rcsb pdb:5LQW|rcsb pdb:5MPS|rcsb pdb:5MQ0|rcsb pdb:5NRL|rcsb pdb:5WSG|rcsb pdb:5Y88|rcsb pdb:5YLZ|rcsb pdb:5ZWM|rcsb pdb:5ZWN|rcsb pdb:5ZWO|rcsb pdb:6BK8|rcsb pdb:6EXN|rcsb pdb:6G90|rcsb pdb:6J6G|rcsb pdb:6J6H|rcsb pdb:6J6N|rcsb pdb:6J6Q|rcsb pdb:6N7P|rcsb pdb:6N7R|rcsb pdb:6N7X|rcsb pdb:7B9V|refseq:NP_014802.3|dip:DIP-1633N|mint:Q12330 - - crc64:B76D72DA8456F3B5 figure legend:Table 1, Suppl. Fig 3B|comment:The spliceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate B act complex 2mM ATP was added and a deleted mutant of actin mRNA was used to stall the splicing reaction.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2016/04/01 - rogid:IiV/AXz4zJjC7A2iuBixxiLYo9E559292 - false mutation:284-1437|tag:1-1|32p radiolabel:?-? - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:Q02521 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-17866|uniprotkb:Q12353|uniprotkb:D6W2K5|ensemblfungi:YOR148C psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:spp2_yeast(display_long)|uniprotkb:SPP2(gene name)|psi-mi:SPP2(display_short)|uniprotkb:Spliceosome maturation protein SPP2(gene name synonym)|uniprotkb:Suppressor of PRP protein 2(gene name synonym)|uniprotkb:YOR148C(locus name) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616300|imex:IM-25038-2 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - refseq:NP_014791.1|sgd:S000005674|go:"GO:0000398"(mRNA splicing, via spliceosome)|go:"GO:0001671"(ATPase activator activity)|go:"GO:0003676"(nucleic acid binding)|go:"GO:0005681"(spliceosomal complex)|go:"GO:0005737"(cytoplasm)|go:"GO:0032781"(positive regulation of ATP-dependent activity)|interpro:IPR000467(D111/G-patch)|interpro:IPR026822|rcsb pdb:7DCO|rcsb pdb:7DCP|rcsb pdb:7DCR|rcsb pdb:7DD3|ensemblfungi:YOR148C(gene)|ensemblfungi:YOR148C_mRNA(transcript)|dip:DIP-4140N|mint:Q02521 - - - figure legend:Table 1, Suppl. Fig 3B|comment:The spliceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate B act complex 2mM ATP was added and a deleted mutant of actin mRNA was used to stall the splicing reaction.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2016/04/01 - rogid:dKji+Gn2/e+tF0W2O37A2HeG4Cc559292 - false mutation:284-1437|tag:1-1|32p radiolabel:?-? - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:P20095 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-13820|ensemblfungi:YNR011C|uniprotkb:D6W1I6 psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:prp2_yeast(display_long)|uniprotkb:PRP2(gene name)|psi-mi:PRP2(display_short)|uniprotkb:RNA2(gene name synonym)|uniprotkb:YNR011C(locus name)|uniprotkb:N2048(orf name)|uniprotkb:Pre-mRNA-processing protein 2(gene name synonym) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616300|imex:IM-25038-2 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - refseq:NP_014408.1|sgd:S000005294|interpro:IPR001650(DNA/RNA helicase, C-terminal)|interpro:IPR002464(DNA/RNA helicase, ATP-dependent, DEAH-box type, conserved site)|interpro:IPR007502(Helicase-associated region)|interpro:IPR011545(DNA/RNA helicase, DEAD/DEAH box type, N-terminal)|interpro:IPR011709(Region of unknown function DUF1605)|interpro:IPR014001(DEAD-like helicase, N-terminal)|interpro:IPR027417|rcsb pdb:5GM6|rcsb pdb:5LQW|rcsb pdb:7DCO|rcsb pdb:7DCP|rcsb pdb:7DCQ|rcsb pdb:7DCR|rcsb pdb:7DD3|go:"GO:0140603"(obsolete ATP hydrolysis activity)|ensemblfungi:YNR011C(gene)|ensemblfungi:YNR011C_mRNA(transcript)|go:"GO:0000349"(generation of catalytic spliceosome for first transesterification step)|go:"GO:0003723"(RNA binding)|go:"GO:0003724"(RNA helicase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0008186"(ATP-dependent activity, acting on RNA)|go:"GO:0034247"(snoRNA splicing)|go:"GO:0071006"(U2-type catalytic step 1 spliceosome)|mint:P20095|dip:DIP-3961N - - crc64:7EB3E1EE93215F57 figure legend:Table 1, Suppl. Fig 3B|comment:The spliceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate B act complex 2mM ATP was added and a deleted mutant of actin mRNA was used to stall the splicing reaction.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2016/04/01 - rogid:wfkc/eidpcEF1HlVabj2p/arPiY559292 - false mutation:284-1437|tag:1-1|32p radiolabel:?-? - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:P49955 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-664|uniprotkb:D6W0B5|ensemblfungi:YMR288W psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:sf3b1_yeast(display_long)|uniprotkb:YMR288W(locus name)|uniprotkb:YM8021.14(orf name)|uniprotkb:HSH155(gene name)|psi-mi:HSH155(display_short) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616300|imex:IM-25038-2 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - refseq:NP_014015.1|sgd:S000004901|dip:DIP-2628N|mint:P49955|ensemblfungi:YMR288W(gene)|ensemblfungi:YMR288W_mRNA(transcript)|go:"GO:0000245"(spliceosomal complex assembly)|go:"GO:0000398"(mRNA splicing, via spliceosome)|go:"GO:0003729"(mRNA binding)|go:"GO:0005686"(U2 snRNP)|go:"GO:0071004"(U2-type prespliceosome)|go:"GO:0071013"(catalytic step 2 spliceosome)|interpro:IPR011989(Armadillo-like helical)|interpro:IPR016024(Armadillo-type fold)|interpro:IPR038737|rcsb pdb:5GM6|rcsb pdb:5LQW|rcsb pdb:5NRL|rcsb pdb:5ZWM|rcsb pdb:5ZWO|rcsb pdb:6G90 - - crc64:27D26E4252A788E2 figure legend:Table 1, Suppl. Fig 3B|comment:The spliceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate B act complex 2mM ATP was added and a deleted mutant of actin mRNA was used to stall the splicing reaction.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2016/04/01 - rogid:t0fwQOOum/AO/xL8vhZdF2EGymo559292 - false mutation:284-1437|tag:1-1|32p radiolabel:?-? - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:Q02554 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-654|uniprotkb:Q04012|uniprotkb:D6W066|ensemblfungi:YMR240C psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:cus1_yeast(display_long)|uniprotkb:YM9408.02C(orf name)|uniprotkb:YMR240C(locus name)|uniprotkb:CUS1(gene name)|psi-mi:CUS1(display_short) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616300|imex:IM-25038-2 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - refseq:NP_013967.1|sgd:S000004853|dip:DIP-910N|mint:Q02554|ensemblfungi:YMR240C(gene)|ensemblfungi:YMR240C_mRNA(transcript)|go:"GO:0000245"(spliceosomal complex assembly)|go:"GO:0000398"(mRNA splicing, via spliceosome)|go:"GO:0003723"(RNA binding)|go:"GO:0005684"(U2-type spliceosomal complex)|go:"GO:0005686"(U2 snRNP)|go:"GO:0071004"(U2-type prespliceosome)|go:"GO:0071011"(precatalytic spliceosome)|go:"GO:0071013"(catalytic step 2 spliceosome)|interpro:IPR006568(PSP, proline-rich)|interpro:IPR007180(Protein of unknown function DUF382)|rcsb pdb:5GM6|rcsb pdb:5LSB|rcsb pdb:5LSL|rcsb pdb:5NRL|rcsb pdb:5ZWM|rcsb pdb:5ZWO|rcsb pdb:6G90 - - crc64:0204B3A666AF7336 figure legend:Table 1, Suppl. Fig 3B|comment:The spliceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate B act complex 2mM ATP was added and a deleted mutant of actin mRNA was used to stall the splicing reaction.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2016/04/01 - rogid:4LtSdsLH2EISJjPGM78b73TD0yw559292 - false mutation:284-1437|tag:1-1|32p radiolabel:?-? - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:Q03654 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-476|uniprotkb:Q6B1D8|ensemblfungi:YMR213W|uniprotkb:D6W038 psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:cef1_yeast(display_long)|uniprotkb:CEF1(gene name)|psi-mi:CEF1(display_short)|uniprotkb:YMR213W(locus name)|uniprotkb:YM8261.07(orf name)|uniprotkb:NTC85(gene name synonym)|uniprotkb:PRP nineteen-associated complex protein 85(gene name synonym)|uniprotkb:PRP19-associated complex protein 85(gene name synonym) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616300|imex:IM-25038-2 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - refseq:NP_013940.1|sgd:S000004826|ensemblfungi:YMR213W(gene)|ensemblfungi:YMR213W_mRNA(transcript)|go:"GO:0000350"(generation of catalytic spliceosome for second transesterification step)|go:"GO:0000398"(mRNA splicing, via spliceosome)|go:"GO:0000974"(Prp19 complex)|go:"GO:0003677"(DNA binding)|go:"GO:0005681"(spliceosomal complex)|go:"GO:0005737"(cytoplasm)|go:"GO:0071006"(U2-type catalytic step 1 spliceosome)|interpro:IPR001005(SANT, DNA-binding)|interpro:IPR009057(Homeodomain-like)|interpro:IPR017930|interpro:IPR021786|rcsb pdb:5GM6|rcsb pdb:5GMK|rcsb pdb:5LJ3|rcsb pdb:5LJ5|rcsb pdb:5LQW|rcsb pdb:5MPS|rcsb pdb:5MQ0|rcsb pdb:5WSG|rcsb pdb:5Y88|rcsb pdb:5YLZ|rcsb pdb:6BK8|rcsb pdb:6EXN|rcsb pdb:6J6G|rcsb pdb:6J6H|rcsb pdb:6J6N|rcsb pdb:6J6Q|rcsb pdb:7DCO|dip:DIP-1113N|mint:Q03654 - - crc64:A4C417A8B330EA9C figure legend:Table 1, Suppl. Fig 3B|comment:The spliceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate B act complex 2mM ATP was added and a deleted mutant of actin mRNA was used to stall the splicing reaction.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2016/04/01 - rogid:AsbV7RtNk+8mCzLk2wZYqbULOFg559292 - false mutation:284-1437|tag:1-1|32p radiolabel:?-? - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:P38302 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-20921|uniprotkb:D6VQI3|ensemblfungi:YBR188C psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:ntc20_yeast(display_long)|uniprotkb:YBR188C(locus name)|uniprotkb:YBR1316(orf name)|uniprotkb:NTC20(gene name)|psi-mi:NTC20(display_short)|uniprotkb:SNT384(gene name synonym)|uniprotkb:PRP19-associated complex protein 20(gene name synonym)|uniprotkb:Synergistic to PRP19 mutation protein 384(gene name synonym) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616300|imex:IM-25038-2 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - refseq:NP_009747.3|sgd:S000000392|mint:P38302|ensemblfungi:YBR188C(gene)|ensemblfungi:YBR188C_mRNA(transcript)|go:"GO:0000398"(mRNA splicing, via spliceosome)|go:"GO:0000974"(Prp19 complex)|go:"GO:0071006"(U2-type catalytic step 1 spliceosome)|dip:DIP-1683N - - crc64:15C5234B25213223 figure legend:Table 1, Suppl. Fig 3B|comment:The spliceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate B act complex 2mM ATP was added and a deleted mutant of actin mRNA was used to stall the splicing reaction.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2016/04/01 - rogid:Ucc4R0lhhhAQfrsXvs+BtCnuJQg559292 - false mutation:284-1437|tag:1-1|32p radiolabel:?-? - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:P53769 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-27128|uniprotkb:D6VYW5|ensemblfungi:YLR323C psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:cwc24_yeast(display_long)|uniprotkb:YLR323C(locus name)|uniprotkb:L8543.3(orf name)|uniprotkb:Complexed with CEF1 protein 24(gene name synonym)|uniprotkb:CWC24(gene name)|psi-mi:CWC24(display_short) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616300|imex:IM-25038-2 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - refseq:NP_013427.1|sgd:S000004315|dip:DIP-1391N|ensemblfungi:YLR323C(gene)|ensemblfungi:YLR323C_mRNA(transcript)|go:"GO:0000349"(generation of catalytic spliceosome for first transesterification step)|go:"GO:0000398"(mRNA splicing, via spliceosome)|go:"GO:0003677"(DNA binding)|go:"GO:0005634"(nucleus)|go:"GO:0005681"(spliceosomal complex)|go:"GO:0005684"(U2-type spliceosomal complex)|go:"GO:0034247"(snoRNA splicing)|go:"GO:0046872"(metal ion binding)|interpro:IPR000571(Zinc finger, CCCH-type)|interpro:IPR001841(Zinc finger, RING-type)|interpro:IPR013083(Zinc finger, RING/FYVE/PHD-type)|interpro:IPR036855|interpro:IPR039971|rcsb pdb:5GM6 - - crc64:4371454CCB6F13D1 figure legend:Table 1, Suppl. Fig 3B|comment:The spliceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate B act complex 2mM ATP was added and a deleted mutant of actin mRNA was used to stall the splicing reaction.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2016/04/01 - rogid:wehPlmPZY5Cp2VSmkkqc3wZifig559292 - false mutation:284-1437|tag:1-1|32p radiolabel:?-? - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:Q06217 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-235|uniprotkb:D6VYS2|ensemblfungi:YLR275W psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:smd2_yeast(display_long)|uniprotkb:SMD2(gene name)|psi-mi:SMD2(display_short)|uniprotkb:YLR275W(locus name)|uniprotkb:L9328.5(orf name)|uniprotkb:snRNP core protein D2(gene name synonym) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616300|imex:IM-25038-2 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - sgd:S000004265|refseq:NP_013377.1|mint:Q06217|dip:DIP-1421N|ensemblfungi:YLR275W(gene)|ensemblfungi:YLR275W_mRNA(transcript)|go:"GO:0000387"(spliceosomal snRNP assembly)|go:"GO:0003723"(RNA binding)|go:"GO:0005682"(U5 snRNP)|go:"GO:0005685"(U1 snRNP)|go:"GO:0005686"(U2 snRNP)|go:"GO:0046540"(U4/U6 x U5 tri-snRNP complex)|go:"GO:0071004"(U2-type prespliceosome)|go:"GO:0071011"(precatalytic spliceosome)|go:"GO:0071013"(catalytic step 2 spliceosome)|interpro:IPR001163(Like-Sm ribonucleoprotein, core)|interpro:IPR010920(Like-Sm ribonucleoprotein-related, core)|interpro:IPR027248|rcsb pdb:3JCM|rcsb pdb:5GAM|rcsb pdb:5GAN|rcsb pdb:5GAO|rcsb pdb:5GM6|rcsb pdb:5GMK|rcsb pdb:5LJ3|rcsb pdb:5LJ5|rcsb pdb:5LQW|rcsb pdb:5MPS|rcsb pdb:5MQ0|rcsb pdb:5NRL|rcsb pdb:5WSG|rcsb pdb:5Y88|rcsb pdb:5YLZ|rcsb pdb:5ZWM|rcsb pdb:5ZWN|rcsb pdb:5ZWO|rcsb pdb:6BK8|rcsb pdb:6EXN|rcsb pdb:6G90|rcsb pdb:6J6G|rcsb pdb:6J6H|rcsb pdb:6J6N|rcsb pdb:6J6Q|rcsb pdb:6N7P|rcsb pdb:6N7R|rcsb pdb:6N7X|rcsb pdb:7B9V - - crc64:7287070B10E090C0 figure legend:Table 1, Suppl. Fig 3B|comment:The spliceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate B act complex 2mM ATP was added and a deleted mutant of actin mRNA was used to stall the splicing reaction.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2016/04/01 - rogid:rglZ3AQuJfa1rFYIeZLqiYj+L4U559292 - false mutation:284-1437|tag:1-1|32p radiolabel:?-? - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:P43321 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-529|ensemblfungi:YLR147C|uniprotkb:D6VYE2|uniprotkb:E9P8W3 psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:smd3_yeast(display_long)|uniprotkb:SMD3(gene name)|psi-mi:SMD3(display_short)|uniprotkb:YLR147C(locus name)|uniprotkb:L9634.6(orf name)|uniprotkb:snRNP core protein D3(gene name synonym) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616300|imex:IM-25038-2 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - refseq:NP_013248.1|sgd:S000004137|rcsb pdb:5YLZ|rcsb pdb:5ZWM|rcsb pdb:5ZWN|rcsb pdb:5ZWO|rcsb pdb:6BK8|rcsb pdb:6EXN|rcsb pdb:6G90|rcsb pdb:6J6G|rcsb pdb:6J6H|rcsb pdb:6J6N|rcsb pdb:6J6Q|rcsb pdb:6N7P|rcsb pdb:6N7R|rcsb pdb:6N7X|rcsb pdb:7B9V|ensemblfungi:YLR147C(gene)|ensemblfungi:YLR147C_mRNA(transcript)|go:"GO:0000243"(commitment complex)|go:"GO:0000387"(spliceosomal snRNP assembly)|go:"GO:0000398"(mRNA splicing, via spliceosome)|go:"GO:0003723"(RNA binding)|go:"GO:0003729"(mRNA binding)|go:"GO:0005682"(U5 snRNP)|go:"GO:0005685"(U1 snRNP)|go:"GO:0005686"(U2 snRNP)|go:"GO:0005687"(U4 snRNP)|go:"GO:0005689"(U12-type spliceosomal complex)|go:"GO:0034715"(pICln-Sm protein complex)|go:"GO:0034719"(SMN-Sm protein complex)|go:"GO:0046540"(U4/U6 x U5 tri-snRNP complex)|go:"GO:0071004"(U2-type prespliceosome)|go:"GO:0071011"(precatalytic spliceosome)|go:"GO:0071013"(catalytic step 2 spliceosome)|go:"GO:0097526"(spliceosomal tri-snRNP complex)|interpro:IPR001163(Like-Sm ribonucleoprotein, core)|interpro:IPR010920(Like-Sm ribonucleoprotein-related, core)|interpro:IPR027141|interpro:IPR034099|rcsb pdb:3JCM|rcsb pdb:5GAM|rcsb pdb:5GAN|rcsb pdb:5GAO|rcsb pdb:5GM6|rcsb pdb:5GMK|rcsb pdb:5LJ3|rcsb pdb:5LJ5|rcsb pdb:5LQW|rcsb pdb:5MPS|rcsb pdb:5MQ0|rcsb pdb:5NRL|rcsb pdb:5WSG|rcsb pdb:5Y88|dip:DIP-715N|mint:P43321 - - crc64:BB02A56C5AD70CF2 figure legend:Table 1, Suppl. Fig 3B|comment:The spliceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate B act complex 2mM ATP was added and a deleted mutant of actin mRNA was used to stall the splicing reaction.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2016/04/01 - rogid:ueJ4D76t8ilvz/p9qMzIGiCzqWk559292 - false mutation:284-1437|tag:1-1|32p radiolabel:?-? - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:Q12309 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-484|uniprotkb:D6VYB5|ensemblfungi:YLR117C psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:clf1_yeast(display_long)|uniprotkb:CLF1(gene name)|psi-mi:CLF1(display_short)|uniprotkb:NTC77(gene name synonym)|uniprotkb:YLR117C(locus name)|uniprotkb:SYF3(gene name synonym)|uniprotkb:L2952(orf name)|uniprotkb:Crooked neck-like factor 1(gene name synonym)|uniprotkb:PRP19-associated complex protein 77(gene name synonym)|uniprotkb:Synthetic lethal with CDC40 protein 3(gene name synonym) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616300|imex:IM-25038-2 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - refseq:NP_013218.1|sgd:S000004107|rcsb pdb:6J6H|rcsb pdb:6J6N|rcsb pdb:6J6Q|rcsb pdb:5GM6|rcsb pdb:5GMK|rcsb pdb:5LJ3|rcsb pdb:5LJ5|rcsb pdb:5LQW|rcsb pdb:5MPS|rcsb pdb:5MQ0|rcsb pdb:5WSG|rcsb pdb:5Y88|rcsb pdb:5YLZ|rcsb pdb:6BK8|rcsb pdb:6EXN|rcsb pdb:6J6G|ensemblfungi:YLR117C(gene)|ensemblfungi:YLR117C_mRNA(transcript)|go:"GO:0000245"(spliceosomal complex assembly)|go:"GO:0000354"(cis assembly of pre-catalytic spliceosome)|go:"GO:0000398"(mRNA splicing, via spliceosome)|go:"GO:0000785"(chromatin)|go:"GO:0000974"(Prp19 complex)|go:"GO:0003682"(chromatin binding)|go:"GO:0003688"(DNA replication origin binding)|go:"GO:0006270"(DNA replication initiation)|go:"GO:0071004"(U2-type prespliceosome)|go:"GO:0071006"(U2-type catalytic step 1 spliceosome)|go:"GO:0071007"(U2-type catalytic step 2 spliceosome)|go:"GO:0071008"(U2-type post-mRNA release spliceosomal complex)|go:"GO:0071014"(post-mRNA release spliceosomal complex)|interpro:IPR003107(RNA-processing protein, HAT helix)|interpro:IPR011990(Tetratricopeptide-like helical)|dip:DIP-1685N|mint:Q12309 - - crc64:AE7D5EC5B3979E00 figure legend:Table 1, Suppl. Fig 3B|comment:The spliceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate B act complex 2mM ATP was added and a deleted mutant of actin mRNA was used to stall the splicing reaction.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2016/04/01 - rogid:VbNHO9YGUushIXHTgscFu4xCzC4559292 - false mutation:284-1437|tag:1-1|32p radiolabel:?-? - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:Q07930 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-27110|uniprotkb:Q06901|uniprotkb:D6VY18|ensemblfungi:YLR016C psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:pml1_yeast(display_long)|uniprotkb:L1591(orf name)|uniprotkb:YLR016C(locus name)|uniprotkb:PML1(gene name)|psi-mi:PML1(display_short) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616300|imex:IM-25038-2 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - refseq:NP_013116.1|sgd:S000004006|dip:DIP-2149N|go:"GO:0005737"(cytoplasm)|go:"GO:0006406"(mRNA export from nucleus)|go:"GO:0051237"(maintenance of RNA location)|go:"GO:0070274"(RES complex)|interpro:IPR000253(Forkhead-associated)|interpro:IPR008984(SMAD/FHA domain)|rcsb pdb:2JKD|rcsb pdb:2MKC|rcsb pdb:2MY3|rcsb pdb:3ELS|rcsb pdb:3ELV|rcsb pdb:5GM6|rcsb pdb:5LQW|rcsb pdb:5ZWM|rcsb pdb:5ZWO|rcsb pdb:7DCO|ensemblfungi:YLR016C(gene)|ensemblfungi:YLR016C_mRNA(transcript)|go:"GO:0000398"(mRNA splicing, via spliceosome)|go:"GO:0003729"(mRNA binding)|go:"GO:0005634"(nucleus) - - crc64:DA39B4465EFFD378 figure legend:Table 1, Suppl. Fig 3B|comment:The spliceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate B act complex 2mM ATP was added and a deleted mutant of actin mRNA was used to stall the splicing reaction.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2016/04/01 - rogid:Q4q9xCj+AyRl430bDbu8yLp/Vak559292 - false mutation:284-1437|tag:1-1|32p radiolabel:?-? - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:P32523 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-493|uniprotkb:Q07870|ensemblfungi:YLL036C|uniprotkb:D6VXW9 psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:prp19_yeast(display_long)|uniprotkb:PRP19(gene name)|psi-mi:PRP19(display_short)|uniprotkb:PSO4(gene name synonym)|uniprotkb:RING-type E3 ubiquitin transferase PRP19(gene name synonym)|uniprotkb:YLL036C(locus name) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616300|imex:IM-25038-2 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - refseq:NP_013064.1|sgd:S000003959|rcsb pdb:6EXN|rcsb pdb:6J6G|rcsb pdb:6J6H|rcsb pdb:6J6N|rcsb pdb:6J6Q|reactome:R-SCE-6781823|reactome:R-SCE-6782135|reactome:R-SCE-6782210|interpro:IPR015943(WD40/YVTN repeat-like)|interpro:IPR038959|rcsb pdb:1N87|rcsb pdb:2BAY|rcsb pdb:3LRV|rcsb pdb:4ZB4|rcsb pdb:5GM6|rcsb pdb:5GMK|rcsb pdb:5LJ5|rcsb pdb:5M8C|rcsb pdb:5MQ0|ensemblfungi:YLL036C(gene)|ensemblfungi:YLL036C_mRNA(transcript)|go:"GO:0000349"(generation of catalytic spliceosome for first transesterification step)|go:"GO:0000974"(Prp19 complex)|go:"GO:0004842"(ubiquitin-protein transferase activity)|go:"GO:0005634"(nucleus)|go:"GO:0005737"(cytoplasm)|go:"GO:0005739"(mitochondrion)|go:"GO:0005829"(cytosol)|go:"GO:0006281"(DNA repair)|go:"GO:0042802"(identical protein binding)|go:"GO:0061630"(ubiquitin protein ligase activity)|go:"GO:0070534"(protein K63-linked ubiquitination)|go:"GO:0071006"(U2-type catalytic step 1 spliceosome)|go:"GO:0071013"(catalytic step 2 spliceosome)|interpro:IPR003613(U box)|interpro:IPR011047(Quinonprotein alcohol dehydrogenase-like)|interpro:IPR013083(Zinc finger, RING/FYVE/PHD-type)|interpro:IPR013915(Pre-mRNA-splicing factor 19)|rcsb pdb:5WSG|rcsb pdb:5Y88|rcsb pdb:5YLZ|rcsb pdb:6BK8|dip:DIP-1112N|mint:P32523 - - crc64:A44D8C7DD6F64185 figure legend:Table 1, Suppl. Fig 3B|comment:The spliceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate B act complex 2mM ATP was added and a deleted mutant of actin mRNA was used to stall the splicing reaction.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2016/04/01 - rogid:/oQc2rJZ7WcCIx7cnqE6XSW/3Vk559292 - false mutation:284-1437|tag:1-1|32p radiolabel:?-? - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:P21374 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-9382|ensemblfungi:YJR050W|uniprotkb:D6VWM1 psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:isy1_yeast(display_long)|uniprotkb:ISY1(gene name)|psi-mi:ISY1(display_short)|uniprotkb:UTR3(gene name synonym)|uniprotkb:YJR050W(locus name)|uniprotkb:J1657(orf name)|uniprotkb:NTC30(gene name synonym)|uniprotkb:Interactor of SYF1(gene name synonym)|uniprotkb:PRP19-associated complex protein 30(gene name synonym) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616300|imex:IM-25038-2 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - ensemblfungi:YJR050W(gene)|ensemblfungi:YJR050W_mRNA(transcript)|go:"GO:0000350"(generation of catalytic spliceosome for second transesterification step)|go:"GO:0000389"(mRNA 3'-splice site recognition)|go:"GO:0000974"(Prp19 complex)|go:"GO:0005737"(cytoplasm)|go:"GO:0071006"(U2-type catalytic step 1 spliceosome)|go:"GO:0071007"(U2-type catalytic step 2 spliceosome)|go:"GO:0071013"(catalytic step 2 spliceosome)|go:"GO:0071014"(post-mRNA release spliceosomal complex)|go:"GO:0071020"(post-spliceosomal complex)|interpro:IPR009360(Isy1-like splicing)|interpro:IPR029012|interpro:IPR037200|rcsb pdb:5GMK|rcsb pdb:5LJ3|rcsb pdb:5LJ5|rcsb pdb:6J6G|rcsb pdb:6J6H|rcsb pdb:6J6N|rcsb pdb:6J6Q|reactome:R-SCE-6781823|reactome:R-SCE-6782135|reactome:R-SCE-6782210|sgd:S000003811|refseq:NP_012584.3|mint:P21374|dip:DIP-1682N - - - figure legend:Table 1, Suppl. Fig 3B|comment:The spliceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate B act complex 2mM ATP was added and a deleted mutant of actin mRNA was used to stall the splicing reaction.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2016/04/01 - rogid:NHLAtd+wLxwtWQD8PsyzyQsfJOg559292 - false mutation:284-1437|tag:1-1|32p radiolabel:?-? - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:P32524 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-603|intact:EBI-464832|uniprotkb:Q6Q5G7|uniprotkb:D6VVZ1|ensemblfungi:YJL203W psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:prp21_yeast(display_long)|uniprotkb:PRP21(gene name)|psi-mi:PRP21(display_short)|uniprotkb:SPP91(gene name synonym)|uniprotkb:YJL203W(locus name)|uniprotkb:J0322(orf name) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616300|imex:IM-25038-2 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - refseq:NP_012332.1|sgd:S000003739|dip:DIP-689N|mint:P32524|ensemblfungi:YJL203W(gene)|ensemblfungi:YJL203W_mRNA(transcript)|go:"GO:0000398"(mRNA splicing, via spliceosome)|go:"GO:0003723"(RNA binding)|go:"GO:0005686"(U2 snRNP)|go:"GO:0071004"(U2-type prespliceosome)|go:"GO:0071013"(catalytic step 2 spliceosome)|interpro:IPR000061(SWAP/Surp)|interpro:IPR022030|interpro:IPR035967|rcsb pdb:4DGW|rcsb pdb:5NRL|rcsb pdb:5ZWM|rcsb pdb:5ZWO|rcsb pdb:6G90|rcsb pdb:7DCO - - crc64:2E9DBA6F06759B3F figure legend:Table 1, Suppl. Fig 3B|comment:The spliceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate B act complex 2mM ATP was added and a deleted mutant of actin mRNA was used to stall the splicing reaction.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2016/04/01 - rogid:mOta2u2zOwtuZB+8s4z9DBL9iI0559292 - false mutation:284-1437|tag:1-1|32p radiolabel:?-? - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:P53333 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-565|uniprotkb:D6VV55|ensemblfungi:YGR278W psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:cwc22_yeast(display_long)|uniprotkb:YGR278W(locus name)|uniprotkb:CWC22(gene name)|psi-mi:CWC22(display_short)|uniprotkb:Complexed with CEF1 protein 22(gene name synonym) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616300|imex:IM-25038-2 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - sgd:S000003510|refseq:NP_011794.3|ensemblfungi:YGR278W(gene)|ensemblfungi:YGR278W_mRNA(transcript)|go:"GO:0000398"(mRNA splicing, via spliceosome)|go:"GO:0003723"(RNA binding)|go:"GO:0005684"(U2-type spliceosomal complex)|go:"GO:0005737"(cytoplasm)|go:"GO:0071013"(catalytic step 2 spliceosome)|interpro:IPR003890(MIF4G-like, type 3)|interpro:IPR003891(Initiation factor eIF-4 gamma, MA3)|interpro:IPR016021(MIF4-like, type 1/2/3)|interpro:IPR016024(Armadillo-type fold)|rcsb pdb:5GM6|rcsb pdb:5GMK|rcsb pdb:5LQW|rcsb pdb:5MPS|rcsb pdb:5MQ0|rcsb pdb:5WSG|rcsb pdb:5YLZ|rcsb pdb:6BK8|rcsb pdb:6EXN|dip:DIP-1869N|mint:P53333|rcsb pdb:6J6H|rcsb pdb:6J6N|rcsb pdb:6J6Q|rcsb pdb:6J6G - - crc64:773E33C71E4A29D0 figure legend:Table 1, Suppl. Fig 3B|comment:The spliceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate B act complex 2mM ATP was added and a deleted mutant of actin mRNA was used to stall the splicing reaction.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2016/04/01 - rogid:V/pQcpK6QPwE9cfqwmQZ0uqGpZk559292 - false mutation:284-1437|tag:1-1|32p radiolabel:?-? - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:P53277 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-23308|uniprotkb:D6VUR1|ensemblfungi:YGR129W psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:syf2_yeast(display_long)|uniprotkb:SYF2(gene name)|psi-mi:SYF2(display_short)|uniprotkb:NTC31(gene name synonym)|uniprotkb:Synthetic lethal with CDC40 protein 2(gene name synonym)|uniprotkb:PRP19 complex protein 31(gene name synonym)|uniprotkb:YGR129W(locus name) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616300|imex:IM-25038-2 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - sgd:S000003361|refseq:NP_011645.3|dip:DIP-1684N|mint:P53277|interpro:IPR013260(mRNA splicing factor SYF2)|ensemblfungi:YGR129W(gene)|rcsb pdb:5GM6|rcsb pdb:5GMK|rcsb pdb:5MPS|rcsb pdb:5MQ0|rcsb pdb:5WSG|rcsb pdb:5Y88|rcsb pdb:5YLZ|rcsb pdb:6BK8|rcsb pdb:6EXN|rcsb pdb:6J6G|rcsb pdb:6J6H|rcsb pdb:6J6N|rcsb pdb:6J6Q|ensemblfungi:YGR129W_mRNA(transcript)|go:"GO:0000398"(mRNA splicing, via spliceosome)|go:"GO:0000974"(Prp19 complex)|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0071004"(U2-type prespliceosome)|go:"GO:0071006"(U2-type catalytic step 1 spliceosome)|go:"GO:0071007"(U2-type catalytic step 2 spliceosome)|go:"GO:0071008"(U2-type post-mRNA release spliceosomal complex) - - - figure legend:Table 1, Suppl. Fig 3B|comment:The spliceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate B act complex 2mM ATP was added and a deleted mutant of actin mRNA was used to stall the splicing reaction.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2016/04/01 - rogid:uVAPXi98XAE2k7IPXvd4d+KNknw559292 - false mutation:284-1437|tag:1-1|32p radiolabel:?-? - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:Q00723 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-841|uniprotkb:D6VUK7|ensemblfungi:YGR075C psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:prp38_yeast(display_long)|uniprotkb:YGR075C(locus name)|uniprotkb:PRP38(gene name)|psi-mi:PRP38(display_short)|uniprotkb:Pre-mRNA-processing factor 38(gene name synonym) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616300|imex:IM-25038-2 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - sgd:S000003307|refseq:NP_011589.3|dip:DIP-1648N|mint:Q00723|ensemblfungi:YGR075C(gene)|ensemblfungi:YGR075C_mRNA(transcript)|go:"GO:0000388"("spliceosome conformational change to release U4 (or U4atac) and U1 (or U11)")|go:"GO:0046540"(U4/U6 x U5 tri-snRNP complex)|go:"GO:0071011"(precatalytic spliceosome)|interpro:IPR005037(PRP38)|rcsb pdb:5NRL|rcsb pdb:5ZWO - - crc64:62EB225947AF4349 figure legend:Table 1, Suppl. Fig 3B|comment:The spliceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate B act complex 2mM ATP was added and a deleted mutant of actin mRNA was used to stall the splicing reaction.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2016/04/01 - rogid:2C+lYBvjAO0jl1CAy0k1Q+Qv7ew559292 - false mutation:284-1437|tag:1-1|32p radiolabel:?-? - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:Q02260 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-585|uniprotkb:D6VUK6|ensemblfungi:YGR074W psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:smd1_yeast(display_long)|uniprotkb:YGR074W(locus name)|uniprotkb:SMD1(gene name)|psi-mi:SMD1(display_short)|uniprotkb:snRNP core protein D1(gene name synonym) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616300|imex:IM-25038-2 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - sgd:S000003306|refseq:NP_011588.3|dip:DIP-227N|mint:Q02260|ensemblfungi:YGR074W(gene)|ensemblfungi:YGR074W_mRNA(transcript)|go:"GO:0000243"(commitment complex)|go:"GO:0000387"(spliceosomal snRNP assembly)|go:"GO:0000398"(mRNA splicing, via spliceosome)|go:"GO:0003723"(RNA binding)|go:"GO:0003729"(mRNA binding)|go:"GO:0005634"(nucleus)|go:"GO:0005682"(U5 snRNP)|go:"GO:0005685"(U1 snRNP)|go:"GO:0005686"(U2 snRNP)|go:"GO:0005689"(U12-type spliceosomal complex)|go:"GO:0005829"(cytosol)|go:"GO:0030532"(small nuclear ribonucleoprotein complex)|go:"GO:0034715"(pICln-Sm protein complex)|go:"GO:0005687"(U4 snRNP)|go:"GO:0034719"(SMN-Sm protein complex)|go:"GO:0046540"(U4/U6 x U5 tri-snRNP complex)|go:"GO:0071004"(U2-type prespliceosome)|go:"GO:0071011"(precatalytic spliceosome)|go:"GO:0071013"(catalytic step 2 spliceosome)|go:"GO:0097526"(spliceosomal tri-snRNP complex)|interpro:IPR001163(Like-Sm ribonucleoprotein, core)|interpro:IPR010920(Like-Sm ribonucleoprotein-related, core)|interpro:IPR027141|rcsb pdb:3JCM|rcsb pdb:5GAM|rcsb pdb:5GAN|rcsb pdb:5GAO|rcsb pdb:5GM6|rcsb pdb:5GMK|rcsb pdb:5LJ3|rcsb pdb:5LJ5|rcsb pdb:5LQW|rcsb pdb:5MPS|rcsb pdb:5MQ0|rcsb pdb:5NRL|rcsb pdb:5WSG|rcsb pdb:5Y88|rcsb pdb:5YLZ|rcsb pdb:5ZWM|rcsb pdb:5ZWN|rcsb pdb:5ZWO|rcsb pdb:6BK8|rcsb pdb:6EXN|rcsb pdb:6G90|rcsb pdb:6J6G|rcsb pdb:6J6H|rcsb pdb:6J6N|rcsb pdb:6J6Q|rcsb pdb:6N7P|rcsb pdb:6N7R|rcsb pdb:6N7X|rcsb pdb:7B9V - - crc64:9929F21EB9218447 figure legend:Table 1, Suppl. Fig 3B|comment:The spliceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate B act complex 2mM ATP was added and a deleted mutant of actin mRNA was used to stall the splicing reaction.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2016/04/01 - rogid:SvOKDbY05xgJXKZ8uS+keXsC41I559292 - false mutation:284-1437|tag:1-1|32p radiolabel:?-? - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:P53207 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-736|uniprotkb:D6VUE9|ensemblfungi:YGR013W psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:snu71_yeast(display_long)|uniprotkb:YGR013W(locus name)|uniprotkb:SNU71(gene name)|psi-mi:SNU71(display_short) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616300|imex:IM-25038-2 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - refseq:NP_011527.1|sgd:S000003245|dip:DIP-5507N|mint:P53207|ensemblfungi:YGR013W(gene)|ensemblfungi:YGR013W_mRNA(transcript)|go:"GO:0000398"(mRNA splicing, via spliceosome)|go:"GO:0003723"(RNA binding)|go:"GO:0005685"(U1 snRNP)|go:"GO:0005737"(cytoplasm)|go:"GO:0071004"(U2-type prespliceosome)|interpro:IPR002483(Splicing factor PWI)|rcsb pdb:5ZWN|rcsb pdb:6G90|rcsb pdb:6N7P|rcsb pdb:6N7R|rcsb pdb:6N7X - - crc64:2659AAA62C4993D5 figure legend:Table 1, Suppl. Fig 3B|comment:The spliceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate B act complex 2mM ATP was added and a deleted mutant of actin mRNA was used to stall the splicing reaction.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2016/04/01 - rogid:/kqpx5vNl2twhBp8xThkSEXX8Wo559292 - false mutation:284-1437|tag:1-1|32p radiolabel:?-? - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:P46947 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-24073|uniprotkb:D6VTX8|ensemblfungi:YGL174W psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:cwc26_yeast(display_long)|uniprotkb:YGL174W(locus name)|uniprotkb:G1642(orf name)|uniprotkb:BUD13(gene name)|psi-mi:BUD13(display_short)|uniprotkb:SLC7(gene name synonym)|uniprotkb:Complexed with CEF1 protein 26(gene name synonym)|uniprotkb:Bud site selection protein 13(gene name synonym)|uniprotkb:Synthetic lethal with CLF1 protein 7(gene name synonym)|uniprotkb:CWC26(gene name synonym) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616300|imex:IM-25038-2 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - sgd:S000003142|refseq:NP_011341.3|dip:DIP-2075N|mint:P46947|ensemblfungi:YGL174W(gene)|ensemblfungi:YGL174W_mRNA(transcript)|go:"GO:0000398"(mRNA splicing, via spliceosome)|go:"GO:0005634"(nucleus)|go:"GO:0005684"(U2-type spliceosomal complex)|go:"GO:0005737"(cytoplasm)|go:"GO:0070274"(RES complex)|interpro:IPR018609|rcsb pdb:2MKC|rcsb pdb:2MY2|rcsb pdb:4UQT|rcsb pdb:5GM6|rcsb pdb:5LQW|rcsb pdb:5ZWM|rcsb pdb:5ZWO - - crc64:1584B390121325CD figure legend:Table 1, Suppl. Fig 3B|comment:The spliceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate B act complex 2mM ATP was added and a deleted mutant of actin mRNA was used to stall the splicing reaction.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2016/04/01 - rogid:JGmiLd4cWl/n2wZVMju48AdYsNk559292 - false mutation:284-1437|tag:1-1|32p radiolabel:?-? - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:P40204 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-637|uniprotkb:D6VTL2|ensemblfungi:YFL017W-A psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:ruxg_yeast(display_long)|uniprotkb:SNP2(gene name synonym)|uniprotkb:YFL017Bw(orf name)|uniprotkb:YFL017W-A(locus name)|uniprotkb:SMX2(gene name)|psi-mi:SMX2(display_short)|uniprotkb:Sm protein G(gene name synonym) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616300|imex:IM-25038-2 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - refseq:NP_116636.1|sgd:S000002965|dip:DIP-6683N|mint:P40204|ensemblfungi:YFL017W-A(gene)|ensemblfungi:YFL017W-A_mRNA(transcript)|go:"GO:0000387"(spliceosomal snRNP assembly)|go:"GO:0000398"(mRNA splicing, via spliceosome)|go:"GO:0003723"(RNA binding)|go:"GO:0005682"(U5 snRNP)|go:"GO:0005685"(U1 snRNP)|go:"GO:0005686"(U2 snRNP)|go:"GO:0005687"(U4 snRNP)|go:"GO:0005689"(U12-type spliceosomal complex)|go:"GO:0034719"(SMN-Sm protein complex)|go:"GO:0046540"(U4/U6 x U5 tri-snRNP complex)|go:"GO:0071004"(U2-type prespliceosome)|go:"GO:0071011"(precatalytic spliceosome)|go:"GO:0071013"(catalytic step 2 spliceosome)|go:"GO:0097526"(spliceosomal tri-snRNP complex)|go:"GO:1990904"(ribonucleoprotein complex)|interpro:IPR001163(Like-Sm ribonucleoprotein, core)|interpro:IPR010920(Like-Sm ribonucleoprotein-related, core)|interpro:IPR034098|interpro:IPR044641|rcsb pdb:3JCM|rcsb pdb:5GAM|rcsb pdb:5GAN|rcsb pdb:5GAO|rcsb pdb:5GM6|rcsb pdb:5GMK|rcsb pdb:5LJ3|rcsb pdb:5LJ5|rcsb pdb:5LQW|rcsb pdb:5MPS|rcsb pdb:5MQ0|rcsb pdb:5NRL|rcsb pdb:5WSG|rcsb pdb:5Y88|rcsb pdb:5YLZ|rcsb pdb:5ZWM|rcsb pdb:5ZWN|rcsb pdb:5ZWO|rcsb pdb:6BK8|rcsb pdb:6EXN|rcsb pdb:6G90|rcsb pdb:6J6G|rcsb pdb:6J6H|rcsb pdb:6J6N|rcsb pdb:6J6Q|rcsb pdb:6N7P|rcsb pdb:6N7R|rcsb pdb:6N7X|rcsb pdb:7B9V|rcsb pdb:7DCO - - crc64:5C6208B8C5A9AB33 figure legend:Table 1, Suppl. Fig 3B|comment:The spliceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate B act complex 2mM ATP was added and a deleted mutant of actin mRNA was used to stall the splicing reaction.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2016/04/01 - rogid:Wh37rGoSJFpJ5o+CuNkBnpiu/5s559292 - false mutation:284-1437|tag:1-1|32p radiolabel:?-? - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:Q03375 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-30409|uniprotkb:D6VTA5|ensemblfungi:YDR482C psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:cwc21_yeast(display_long)|uniprotkb:YDR482C(locus name)|uniprotkb:Complexed with CEF1 protein 21(gene name synonym)|uniprotkb:CWC21(gene name)|psi-mi:CWC21(display_short) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616300|imex:IM-25038-2 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - sgd:S000002890|refseq:NP_010770.3|dip:DIP-1847N|mint:Q03375|ensemblfungi:YDR482C(gene)|ensemblfungi:YDR482C_mRNA(transcript)|go:"GO:0000398"(mRNA splicing, via spliceosome)|go:"GO:0005684"(U2-type spliceosomal complex)|go:"GO:0005737"(cytoplasm)|interpro:IPR013170(mRNA splicing factor, Cwf21)|rcsb pdb:5GM6|rcsb pdb:5GMK|rcsb pdb:5LJ3|rcsb pdb:5LJ5|rcsb pdb:5MPS|rcsb pdb:5MQ0|rcsb pdb:5WSG|rcsb pdb:5YLZ|rcsb pdb:6BK8|rcsb pdb:6EXN|rcsb pdb:6J6G|rcsb pdb:6J6H|rcsb pdb:6J6N|rcsb pdb:6J6Q - - crc64:9723E1C0A393508B figure legend:Table 1, Suppl. Fig 3B|comment:The spliceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate B act complex 2mM ATP was added and a deleted mutant of actin mRNA was used to stall the splicing reaction.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2016/04/01 - rogid:dXmh6RsUTTtGZ8d1CnRgs1Ma+VI559292 - false mutation:284-1437|tag:1-1|32p radiolabel:?-? - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:Q04048 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-540|uniprotkb:D6VT47|ensemblfungi:YDR416W psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:syf1_yeast(display_long)|uniprotkb:SYF1(gene name)|psi-mi:SYF1(display_short)|uniprotkb:NTC90(gene name synonym)|uniprotkb:Synthetic lethal with CDC40 protein 1(gene name synonym)|uniprotkb:PRP19-associated complex protein 90(gene name synonym)|uniprotkb:YDR416W(locus name) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616300|imex:IM-25038-2 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - refseq:NP_010704.1|sgd:S000002824|rcsb pdb:5YLZ|rcsb pdb:6BK8|rcsb pdb:6EXN|rcsb pdb:6J6G|rcsb pdb:6J6H|rcsb pdb:6J6N|rcsb pdb:6J6Q|reactome:R-SCE-6781823|reactome:R-SCE-6782135|reactome:R-SCE-6782210|rcsb pdb:5Y88|ensemblfungi:YDR416W(gene)|ensemblfungi:YDR416W_mRNA(transcript)|go:"GO:0000349"(generation of catalytic spliceosome for first transesterification step)|go:"GO:0000398"(mRNA splicing, via spliceosome)|go:"GO:0000974"(Prp19 complex)|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0071004"(U2-type prespliceosome)|go:"GO:0071006"(U2-type catalytic step 1 spliceosome)|go:"GO:0071007"(U2-type catalytic step 2 spliceosome)|go:"GO:0071008"(U2-type post-mRNA release spliceosomal complex)|go:"GO:0071014"(post-mRNA release spliceosomal complex)|interpro:IPR003107(RNA-processing protein, HAT helix)|interpro:IPR011990(Tetratricopeptide-like helical)|rcsb pdb:5GM6|rcsb pdb:5GMK|rcsb pdb:5LJ3|rcsb pdb:5LJ5|rcsb pdb:5LQW|rcsb pdb:5MPS|rcsb pdb:5MQ0|rcsb pdb:5WSG|dip:DIP-1681N|mint:Q04048 - - crc64:ED4368A624A40E7C figure legend:Table 1, Suppl. Fig 3B|comment:The spliceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate B act complex 2mM ATP was added and a deleted mutant of actin mRNA was used to stall the splicing reaction.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2016/04/01 - rogid:+1TwiqsQv1IcWxuoIWcJGtTCUFU559292 - false mutation:284-1437|tag:1-1|32p radiolabel:?-? - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:P40968 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-13834|uniprotkb:Q06341|ensemblfungi:YDR364C|uniprotkb:D6VSZ3 psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:prp17_yeast(display_long)|uniprotkb:CDC40(gene name)|psi-mi:CDC40(display_short)|uniprotkb:PRP17(gene name synonym)|uniprotkb:SLU4(gene name synonym)|uniprotkb:XRS2(gene name synonym)|uniprotkb:YDR364C(locus name)|uniprotkb:D9481.11(orf name)|uniprotkb:Cell division control protein 40(gene name synonym) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616300|imex:IM-25038-2 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - ensemblfungi:YDR364C(gene)|ensemblfungi:YDR364C_mRNA(transcript)|go:"GO:0000348"(mRNA branch site recognition)|go:"GO:0000350"(generation of catalytic spliceosome for second transesterification step)|go:"GO:0000389"(mRNA 3'-splice site recognition)|go:"GO:0005681"(spliceosomal complex)|go:"GO:0034399"(nuclear periphery)|go:"GO:0071013"(catalytic step 2 spliceosome)|interpro:IPR001680(WD40 repeat)|interpro:IPR015943(WD40/YVTN repeat-like)|interpro:IPR020472|interpro:IPR032847|interpro:IPR036322|rcsb pdb:5GM6|rcsb pdb:5GMK|rcsb pdb:5MPS|rcsb pdb:5MQ0|rcsb pdb:5WSG|rcsb pdb:5Y88|rcsb pdb:5YLZ|rcsb pdb:6BK8|rcsb pdb:6EXN|rcsb pdb:6J6G|rcsb pdb:6J6H|rcsb pdb:6J6N|rcsb pdb:6J6Q|sgd:S000002772|refseq:NP_010652.3|dip:DIP-1111N - - crc64:40AED5DB35319147 figure legend:Table 1, Suppl. Fig 3B|comment:The spliceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate B act complex 2mM ATP was added and a deleted mutant of actin mRNA was used to stall the splicing reaction.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2016/04/01 - rogid:KoJjWDas71e2U/bQRs+yQFpbTk4559292 - false mutation:284-1437|tag:1-1|32p radiolabel:?-? - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:Q03772 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-36780|uniprotkb:D6VSE3|ensemblfungi:YDR163W psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:cwc15_yeast(display_long)|uniprotkb:CWC15(gene name)|psi-mi:CWC15(display_short)|uniprotkb:Complexed with CEF1 protein 15(gene name synonym)|uniprotkb:YDR163W(locus name)|uniprotkb:YD8358.17(orf name) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616300|imex:IM-25038-2 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - sgd:S000002570|interpro:IPR006973(Cwf15/Cwc15 cell cycle control protein)|refseq:NP_010447.3|rcsb pdb:6J6G|rcsb pdb:6J6H|rcsb pdb:6J6N|rcsb pdb:6J6Q|rcsb pdb:5MPS|rcsb pdb:5MQ0|rcsb pdb:5WSG|rcsb pdb:5GM6|rcsb pdb:5GMK|rcsb pdb:5LJ3|rcsb pdb:6BK8|rcsb pdb:6EXN|rcsb pdb:5Y88|rcsb pdb:5YLZ|ensemblfungi:YDR163W(gene)|ensemblfungi:YDR163W_mRNA(transcript)|go:"GO:0000398"(mRNA splicing, via spliceosome)|go:"GO:0003723"(RNA binding)|go:"GO:0005634"(nucleus)|go:"GO:0005684"(U2-type spliceosomal complex)|go:"GO:0045292"(mRNA cis splicing, via spliceosome)|go:"GO:0071013"(catalytic step 2 spliceosome)|rcsb pdb:7DCO - - - figure legend:Table 1, Suppl. Fig 3B|comment:The spliceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate B act complex 2mM ATP was added and a deleted mutant of actin mRNA was used to stall the splicing reaction.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2016/04/01 - rogid:raWAr5hiD2qEEeUG/lcMNpo1lhM559292 - false mutation:284-1437|tag:1-1|32p radiolabel:?-? - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:Q12046 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-553|ensemblfungi:YDL209C|uniprotkb:D6VRE5 psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:cwc2_yeast(display_long)|uniprotkb:CWC2(gene name)|psi-mi:CWC2(display_short)|uniprotkb:YDL209C(locus name)|uniprotkb:D1041(orf name)|uniprotkb:NTC40(gene name synonym)|uniprotkb:SLC3(gene name synonym)|uniprotkb:Complexed with CEF1 protein 2(gene name synonym)|uniprotkb:PRP19-associated complex protein 40(gene name synonym)|uniprotkb:Synthetic lethal with CLF1 protein 3(gene name synonym) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616300|imex:IM-25038-2 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - interpro:IPR000571(Zinc finger, CCCH-type)|interpro:IPR012677(Nucleotide-binding, alpha-beta plait)|interpro:IPR032297|interpro:IPR034181|interpro:IPR035979|interpro:IPR039171|interpro:IPR039173|rcsb pdb:3TP2|rcsb pdb:3U1L|rcsb pdb:3U1M|rcsb pdb:5GM6|rcsb pdb:5GMK|rcsb pdb:5LJ3|rcsb pdb:5LJ5|rcsb pdb:5LQW|rcsb pdb:5MPS|rcsb pdb:5MQ0|rcsb pdb:5WSG|rcsb pdb:5Y88|rcsb pdb:5YLZ|rcsb pdb:6BK8|rcsb pdb:6EXN|rcsb pdb:6J6G|rcsb pdb:6J6H|rcsb pdb:6J6N|refseq:NP_010072.1|sgd:S000002368|ensemblfungi:YDL209C(gene)|ensemblfungi:YDL209C_mRNA(transcript)|go:"GO:0000387"(spliceosomal snRNP assembly)|go:"GO:0000398"(mRNA splicing, via spliceosome)|go:"GO:0000974"(Prp19 complex)|go:"GO:0007049"(cell cycle)|go:"GO:0017070"(U6 snRNA binding)|go:"GO:0033120"(positive regulation of RNA splicing)|go:"GO:0036002"(pre-mRNA binding)|go:"GO:0045292"(mRNA cis splicing, via spliceosome)|go:"GO:0045787"(positive regulation of cell cycle)|go:"GO:0046872"(metal ion binding)|go:"GO:0071006"(U2-type catalytic step 1 spliceosome)|go:"GO:0071007"(U2-type catalytic step 2 spliceosome)|interpro:IPR000504(RNA recognition motif, RNP-1)|rcsb pdb:6J6Q|dip:DIP-5164N - - crc64:371734A11DFDB339 figure legend:Table 1, Suppl. Fig 3B|comment:The spliceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate B act complex 2mM ATP was added and a deleted mutant of actin mRNA was used to stall the splicing reaction.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2016/04/01 - rogid:bCvRFfQxZ4QaBZqqOyQP7ye/UeQ559292 - false mutation:284-1437|tag:1-1|32p radiolabel:?-? - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:Q07350 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-688|uniprotkb:Q05434|ensemblfungi:YDL043C|uniprotkb:D6VRV3 psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:prp11_yeast(display_long)|uniprotkb:PRP11(gene name)|psi-mi:PRP11(display_short)|uniprotkb:PRP10(gene name synonym)|uniprotkb:RNA11(gene name synonym)|uniprotkb:YDL043C(locus name)|uniprotkb:D2711(orf name) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616300|imex:IM-25038-2 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - refseq:NP_010241.1|sgd:S000002201|ensemblfungi:YDL043C(gene)|ensemblfungi:YDL043C_mRNA(transcript)|go:"GO:0000245"(spliceosomal complex assembly)|go:"GO:0003723"(RNA binding)|go:"GO:0005686"(U2 snRNP)|go:"GO:0008270"(zinc ion binding)|go:"GO:0071004"(U2-type prespliceosome)|go:"GO:0071013"(catalytic step 2 spliceosome)|interpro:IPR003604(Zinc finger, U1-type)|interpro:IPR031781|interpro:IPR036236|rcsb pdb:4DGW|rcsb pdb:5GM6|rcsb pdb:5NRL|rcsb pdb:5ZWM|rcsb pdb:5ZWO|rcsb pdb:6G90|dip:DIP-1001N|mint:Q07350 - - crc64:D8BA3BB27F36F06F figure legend:Table 1, Suppl. Fig 3B|comment:The spliceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate B act complex 2mM ATP was added and a deleted mutant of actin mRNA was used to stall the splicing reaction.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2016/04/01 - rogid:xyunpB0FKbtjXifS4hyBHPmL4zs559292 - false mutation:284-1437|tag:1-1|32p radiolabel:?-? - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:P19736 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-511|ensemblfungi:YDL030W|uniprotkb:D6VRW2 psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:prp9_yeast(display_long)|uniprotkb:PRP9(gene name)|psi-mi:PRP9(display_short)|uniprotkb:D2773(orf name)|uniprotkb:YDL030W(locus name) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616300|imex:IM-25038-2 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - ensemblfungi:YDL030W(gene)|ensemblfungi:YDL030W_mRNA(transcript)|go:"GO:0000395"(mRNA 5'-splice site recognition)|go:"GO:0000398"(mRNA splicing, via spliceosome)|go:"GO:0003723"(RNA binding)|go:"GO:0005681"(spliceosomal complex)|go:"GO:0008270"(zinc ion binding)|go:"GO:0071004"(U2-type prespliceosome)|interpro:IPR000690(Zinc finger, C2H2-type matrin)|interpro:IPR003604(Zinc finger, U1-type)|interpro:IPR013087(Zinc finger, C2H2-type/integrase, DNA-binding)|interpro:IPR022755|interpro:IPR024598|interpro:IPR031590|interpro:IPR031774|interpro:IPR031776|interpro:IPR036236|refseq:NP_010254.1|sgd:S000002188|rcsb pdb:4DGW|rcsb pdb:5NRL|rcsb pdb:5ZWM|rcsb pdb:5ZWO|rcsb pdb:6G90|dip:DIP-651N|mint:P19736 - - crc64:9020885E620488F3 figure legend:Table 1, Suppl. Fig 3B|comment:The spliceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate B act complex 2mM ATP was added and a deleted mutant of actin mRNA was used to stall the splicing reaction.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2016/04/01 - rogid:gdiY5/Azr3xdR4IiaMsyAcxJyXA559292 - false mutation:284-1437|tag:1-1|32p radiolabel:?-? - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:P36048 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-243|uniprotkb:D6VX27|ensemblfungi:YKL173W psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:sn114_yeast(display_long)|uniprotkb:GIN10(gene name synonym)|uniprotkb:YKL173W(locus name)|uniprotkb:YKL637(orf name)|uniprotkb:SNU114(gene name)|psi-mi:SNU114(display_short)|uniprotkb:Growth inhibitory protein 10(gene name synonym)|uniprotkb:114 kDa U5 small nuclear ribonucleoprotein component(gene name synonym) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616300|imex:IM-25038-2 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - refseq:NP_012748.1|sgd:S000001656|mint:P36048|dip:DIP-759N|ensemblfungi:YKL173W(gene)|ensemblfungi:YKL173W_mRNA(transcript)|go:"GO:0000244"(spliceosomal tri-snRNP complex assembly)|go:"GO:0000349"(generation of catalytic spliceosome for first transesterification step)|go:"GO:0000388"("spliceosome conformational change to release U4 (or U4atac) and U1 (or U11)")|go:"GO:0000398"(mRNA splicing, via spliceosome)|go:"GO:0003924"(GTPase activity)|go:"GO:0005525"(GTP binding)|go:"GO:0005634"(nucleus)|go:"GO:0005682"(U5 snRNP)|go:"GO:0030623"(U5 snRNA binding)|go:"GO:0046540"(U4/U6 x U5 tri-snRNP complex)|go:"GO:0071007"(U2-type catalytic step 2 spliceosome)|go:"GO:1990904"(ribonucleoprotein complex)|interpro:IPR000640(Translation elongation factor EFG/EF2, C-terminal)|interpro:IPR000795(Protein synthesis factor, GTP-binding)|interpro:IPR004161(Translation elongation factor EFTu/EF1A, domain 2)|interpro:IPR005517(Translation elongation factor EFG/EF2, domain IV)|interpro:IPR009000(Translation elongation and initiation factors/Ribosomal, beta-barrel)|interpro:IPR014721(Ribosomal protein S5 domain 2-type fold)|interpro:IPR020568|interpro:IPR027417|interpro:IPR031950|interpro:IPR035647|interpro:IPR035655|interpro:IPR044121|rcsb pdb:3JCM|rcsb pdb:5GAM|rcsb pdb:5GAN|rcsb pdb:5GM6|rcsb pdb:5GMK|rcsb pdb:5LJ3|rcsb pdb:5LJ5|rcsb pdb:5LQW|rcsb pdb:5MPS|rcsb pdb:5MQ0|rcsb pdb:5NRL|rcsb pdb:5WSG|rcsb pdb:5Y88|rcsb pdb:5YLZ|rcsb pdb:5ZWM|rcsb pdb:5ZWO|rcsb pdb:6BK8|rcsb pdb:6EXN|rcsb pdb:6J6G|rcsb pdb:6J6H|rcsb pdb:6J6N|rcsb pdb:6J6Q|rcsb pdb:6TEO - - crc64:5CE30CEFF154D013 figure legend:Table 1, Suppl. Fig 3B|comment:The spliceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate B act complex 2mM ATP was added and a deleted mutant of actin mRNA was used to stall the splicing reaction.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2016/04/01 - rogid:eHDUPadk87+Qb525xpL1tMWb3S4559292 - false mutation:284-1437|tag:1-1|32p radiolabel:?-? - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:P28320 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-26795|uniprotkb:D6VXJ3|ensemblfungi:YKL095W psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:yju2_yeast(display_long)|uniprotkb:YKL095W(locus name)|uniprotkb:YKL442(orf name)|uniprotkb:YJU2(gene name)|psi-mi:YJU2(display_short)|uniprotkb:CWC16(gene name synonym) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616300|imex:IM-25038-2 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - refseq:NP_012828.1|sgd:S000001578|mint:P28320|dip:DIP-1915N|ensemblfungi:YKL095W(gene)|ensemblfungi:YKL095W_mRNA(transcript)|go:"GO:0000349"(generation of catalytic spliceosome for first transesterification step)|go:"GO:0000350"(generation of catalytic spliceosome for second transesterification step)|go:"GO:0005634"(nucleus)|go:"GO:0008380"(RNA splicing)|go:"GO:0030620"(U2 snRNA binding)|go:"GO:0046872"(metal ion binding)|go:"GO:0071006"(U2-type catalytic step 1 spliceosome)|go:"GO:0071007"(U2-type catalytic step 2 spliceosome)|interpro:IPR007590(Protein of unknown function DUF572)|interpro:IPR043701|rcsb pdb:5GMK|rcsb pdb:5LJ3|rcsb pdb:5LJ5|rcsb pdb:5Y88|rcsb pdb:6EXN|rcsb pdb:6J6Q - - crc64:B57F3A173A2113FD figure legend:Table 1, Suppl. Fig 3B|comment:The spliceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate B act complex 2mM ATP was added and a deleted mutant of actin mRNA was used to stall the splicing reaction.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2016/04/01 - rogid:RB1yjFkujiRVEYwnEtk3x0Beo88559292 - false mutation:284-1437|tag:1-1|32p radiolabel:?-? - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:P33203 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-701|uniprotkb:D6VXS4|ensemblfungi:YKL012W psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:prp40_yeast(display_long)|uniprotkb:YKL165(orf name)|uniprotkb:YKL012W(locus name)|uniprotkb:PRP40(gene name)|psi-mi:PRP40(display_short) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616300|imex:IM-25038-2 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - sgd:S000001495|refseq:NP_012913.3|ensemblfungi:YKL012W(gene)|ensemblfungi:YKL012W_mRNA(transcript)|go:"GO:0000398"(mRNA splicing, via spliceosome)|go:"GO:0003723"(RNA binding)|go:"GO:0005634"(nucleus)|go:"GO:0005685"(U1 snRNP)|go:"GO:0045292"(mRNA cis splicing, via spliceosome)|go:"GO:0071004"(U2-type prespliceosome)|interpro:IPR001202(WW/Rsp5/WWP)|interpro:IPR002713(FF)|interpro:IPR036020|interpro:IPR036517|interpro:IPR039726|rcsb pdb:1O6W|rcsb pdb:2B7E|rcsb pdb:2KFD|rcsb pdb:6N7P|dip:DIP-1620N|mint:P33203 - - crc64:BA1C1C91D532524C figure legend:Table 1, Suppl. Fig 3B|comment:The spliceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate B act complex 2mM ATP was added and a deleted mutant of actin mRNA was used to stall the splicing reaction.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2016/04/01 - rogid:y4tc4ChQBe85CA6638S4K0Bt1U8559292 - false mutation:284-1437|tag:1-1|32p radiolabel:?-? - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:P40567 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-789|uniprotkb:D6VVT9|ensemblfungi:YIR009W psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:msl1_yeast(display_long)|uniprotkb:YIB9W(orf name)|uniprotkb:YIR009W(locus name)|uniprotkb:MSL1(gene name)|psi-mi:MSL1(display_short) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616300|imex:IM-25038-2 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - refseq:NP_012274.1|sgd:S000001448|rcsb pdb:6BK8|ensemblfungi:YIR009W(gene)|ensemblfungi:YIR009W_mRNA(transcript)|go:"GO:0000398"(mRNA splicing, via spliceosome)|go:"GO:0005685"(U1 snRNP)|go:"GO:0030619"(U1 snRNA binding)|go:"GO:0030620"(U2 snRNA binding)|go:"GO:0071004"(U2-type prespliceosome)|interpro:IPR000504(RNA recognition motif, RNP-1)|interpro:IPR012677(Nucleotide-binding, alpha-beta plait)|interpro:IPR035979|rcsb pdb:5GMK|rcsb pdb:5LJ3|rcsb pdb:5LJ5|rcsb pdb:5MQ0|rcsb pdb:5NRL|rcsb pdb:5WSG|rcsb pdb:5Y88|rcsb pdb:5YLZ|rcsb pdb:5ZWM|rcsb pdb:5ZWO|dip:DIP-816N|mint:P40567|rcsb pdb:6EXN|rcsb pdb:6G90|rcsb pdb:6J6G|rcsb pdb:6J6H|rcsb pdb:6J6N|rcsb pdb:6J6Q - - - figure legend:Table 1, Suppl. Fig 3B|comment:The spliceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate B act complex 2mM ATP was added and a deleted mutant of actin mRNA was used to stall the splicing reaction.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2016/04/01 - rogid:GAiHZKcbfNjbem3+i1cZXFm4NfE559292 - false mutation:284-1437|tag:1-1|32p radiolabel:?-? - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:P40565 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-25387|uniprotkb:D6VVT5|ensemblfungi:YIR005W psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:ist3_yeast(display_long)|uniprotkb:IST3(gene name)|psi-mi:IST3(display_short)|uniprotkb:SNU17(gene name synonym)|uniprotkb:YIR005W(locus name)|uniprotkb:YIB5W(orf name)|uniprotkb:U2 snRNP protein SNU17(gene name synonym)|uniprotkb:Increased sodium tolerance protein 3(gene name synonym) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616300|imex:IM-25038-2 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - refseq:NP_012270.1|sgd:S000001444|dip:DIP-2076N|ensemblfungi:YIR005W(gene)|ensemblfungi:YIR005W_mRNA(transcript)|go:"GO:0000245"(spliceosomal complex assembly)|go:"GO:0000349"(generation of catalytic spliceosome for first transesterification step)|go:"GO:0000398"(mRNA splicing, via spliceosome)|go:"GO:0003723"(RNA binding)|go:"GO:0005686"(U2 snRNP)|go:"GO:0005737"(cytoplasm)|go:"GO:0006406"(mRNA export from nucleus)|go:"GO:0070274"(RES complex)|go:"GO:0071011"(precatalytic spliceosome)|interpro:IPR000504(RNA recognition motif, RNP-1)|interpro:IPR012677(Nucleotide-binding, alpha-beta plait)|interpro:IPR035979|rcsb pdb:2MKC|rcsb pdb:2MY2|rcsb pdb:2MY3|rcsb pdb:4UQT|rcsb pdb:5GM6|rcsb pdb:5LQW|rcsb pdb:5ZWM|rcsb pdb:5ZWO - - crc64:9D51C3043AB823D2 figure legend:Table 1, Suppl. Fig 3B|comment:The spliceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate B act complex 2mM ATP was added and a deleted mutant of actin mRNA was used to stall the splicing reaction.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2016/04/01 - rogid:UlibOhZ4aPt5J8Ptg+Z1zA1VJXs559292 - false mutation:284-1437|tag:1-1|32p radiolabel:?-? - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:P33334 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-465|uniprotkb:D3DLB4|ensemblfungi:YHR165C psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:prp8_yeast(display_long)|uniprotkb:PRP8(gene name)|psi-mi:PRP8(display_short)|uniprotkb:DBF3(gene name synonym)|uniprotkb:DNA39(gene name synonym)|uniprotkb:RNA8(gene name synonym)|uniprotkb:SLT21(gene name synonym)|uniprotkb:USA2(gene name synonym)|uniprotkb:YHR165C(locus name) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616300|imex:IM-25038-2 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - refseq:NP_012035.1|sgd:S000001208|rcsb pdb:4ILJ|rcsb pdb:5DCA|rcsb pdb:5GAM|rcsb pdb:5GAN|rcsb pdb:5GAO|rcsb pdb:5GAP|rcsb pdb:5GM6|rcsb pdb:5GMK|rcsb pdb:5LJ3|rcsb pdb:5LJ5|rcsb pdb:5LQW|rcsb pdb:5M52|rcsb pdb:5M5P|rcsb pdb:5MPS|rcsb pdb:5MQ0|rcsb pdb:5NRL|rcsb pdb:5QY1|rcsb pdb:5QY2|rcsb pdb:5QY3|rcsb pdb:5QY4|rcsb pdb:5QY5|rcsb pdb:5QY6|rcsb pdb:5QY7|rcsb pdb:5QY8|rcsb pdb:5QY9|rcsb pdb:5QYA|rcsb pdb:5QYB|rcsb pdb:5QYC|rcsb pdb:5QYD|rcsb pdb:5QYE|rcsb pdb:5QYF|rcsb pdb:5QYG|rcsb pdb:5QYH|rcsb pdb:5QYI|rcsb pdb:5QYJ|rcsb pdb:5QYK|rcsb pdb:5QYL|rcsb pdb:5QYM|rcsb pdb:5QYN|rcsb pdb:5QYO|rcsb pdb:5QYP|rcsb pdb:5QYQ|rcsb pdb:5QYR|rcsb pdb:5QYS|rcsb pdb:5QYT|rcsb pdb:5QYU|rcsb pdb:5QYV|rcsb pdb:5QYW|rcsb pdb:5QYX|rcsb pdb:5QYY|rcsb pdb:5QYZ|rcsb pdb:5QZ0|rcsb pdb:5QZ1|rcsb pdb:5QZ2|rcsb pdb:5QZ3|rcsb pdb:5QZ4|rcsb pdb:5QZ5|rcsb pdb:5QZ6|rcsb pdb:5QZ7|rcsb pdb:5QZ8|rcsb pdb:5QZ9|rcsb pdb:5QZA|rcsb pdb:5QZB|rcsb pdb:5QZC|rcsb pdb:5QZD|rcsb pdb:5QZE|rcsb pdb:5QZF|rcsb pdb:5QZG|rcsb pdb:5QZH|rcsb pdb:5QZI|rcsb pdb:5QZJ|rcsb pdb:5QZK|rcsb pdb:5QZL|rcsb pdb:5QZM|rcsb pdb:5QZN|rcsb pdb:5QZO|rcsb pdb:5QZP|rcsb pdb:5R14|rcsb pdb:5R15|rcsb pdb:5R16|rcsb pdb:5R17|rcsb pdb:5R18|rcsb pdb:5R19|rcsb pdb:5R1A|rcsb pdb:5R1B|rcsb pdb:5R1C|rcsb pdb:5R1D|rcsb pdb:5R1E|rcsb pdb:5R1F|rcsb pdb:5R1G|rcsb pdb:5R1H|rcsb pdb:5R1I|rcsb pdb:5R1J|rcsb pdb:5R1K|rcsb pdb:5R1L|rcsb pdb:5R1M|rcsb pdb:5R1N|rcsb pdb:5R1O|rcsb pdb:5R1P|rcsb pdb:5R1Q|rcsb pdb:5R1S|rcsb pdb:5WSG|rcsb pdb:5Y88|rcsb pdb:5YLZ|rcsb pdb:5ZWM|rcsb pdb:5ZWO|rcsb pdb:6BK8|rcsb pdb:6EXN|rcsb pdb:6J6G|rcsb pdb:6J6H|rcsb pdb:6J6N|rcsb pdb:6J6Q|rcsb pdb:6TEO|rcsb pdb:7B9V|rcsb pdb:5QZQ|rcsb pdb:5QZR|rcsb pdb:5QZS|rcsb pdb:5QZT|rcsb pdb:5QZU|rcsb pdb:5QZV|rcsb pdb:5QZW|rcsb pdb:5QZX|rcsb pdb:5QZY|rcsb pdb:5QZZ|rcsb pdb:5R00|rcsb pdb:5R01|rcsb pdb:5R02|rcsb pdb:5R03|rcsb pdb:5R04|rcsb pdb:5R05|rcsb pdb:5R06|rcsb pdb:5R07|rcsb pdb:5R08|rcsb pdb:5R09|rcsb pdb:5R0A|rcsb pdb:5R0B|rcsb pdb:5R0C|rcsb pdb:5R0D|rcsb pdb:5R0E|rcsb pdb:5R0F|rcsb pdb:5R0G|rcsb pdb:5R0H|rcsb pdb:5R0I|rcsb pdb:5R0J|rcsb pdb:5R0K|rcsb pdb:5R0L|rcsb pdb:5R0M|rcsb pdb:5R0N|rcsb pdb:5R0O|rcsb pdb:5R0P|rcsb pdb:5R0Q|rcsb pdb:5R0R|rcsb pdb:5R0S|rcsb pdb:5R0T|rcsb pdb:5R0U|rcsb pdb:5R0V|rcsb pdb:5R0W|rcsb pdb:5R0X|rcsb pdb:5R0Y|rcsb pdb:5R0Z|rcsb pdb:5R10|rcsb pdb:5R11|rcsb pdb:5R12|rcsb pdb:5R13|ensemblfungi:YHR165C(gene)|ensemblfungi:YHR165C_mRNA(transcript)|go:"GO:0000244"(spliceosomal tri-snRNP complex assembly)|go:"GO:0000350"(generation of catalytic spliceosome for second transesterification step)|go:"GO:0000389"(mRNA 3'-splice site recognition)|go:"GO:0000395"(mRNA 5'-splice site recognition)|go:"GO:0003729"(mRNA binding)|go:"GO:0005634"(nucleus)|go:"GO:0005682"(U5 snRNP)|go:"GO:0008237"(metallopeptidase activity)|go:"GO:0017070"(U6 snRNA binding)|go:"GO:0030619"(U1 snRNA binding)|go:"GO:0030620"(U2 snRNA binding)|go:"GO:0030623"(U5 snRNA binding)|go:"GO:0046540"(U4/U6 x U5 tri-snRNP complex)|go:"GO:0070122"(isopeptidase activity)|go:"GO:0071013"(catalytic step 2 spliceosome)|rcsb pdb:3SBT|rcsb pdb:3ZEF|rcsb pdb:4BGD|rcsb pdb:4I43|go:"GO:0097157"(pre-mRNA intronic binding)|interpro:IPR000555(Mov34/MPN/PAD-1)|interpro:IPR012337(Polynucleotidyl transferase, Ribonuclease H fold)|interpro:IPR012591(PRO8NT)|interpro:IPR012592(PROCN)|interpro:IPR012984(PRO, C-terminal)|interpro:IPR019580|interpro:IPR019581|interpro:IPR019582|interpro:IPR021983|interpro:IPR027652|interpro:IPR037518|interpro:IPR042516|interpro:IPR043172|interpro:IPR043173|rcsb pdb:2OG4|rcsb pdb:3E66|rcsb pdb:3E9O|rcsb pdb:3E9P|rcsb pdb:3JCM|rcsb pdb:3SBG|rcsb pdb:4ILG|rcsb pdb:4ILH|dip:DIP-2427N|mint:P33334 - - crc64:8F4F6F89D34D3508 figure legend:Table 1, Suppl. Fig 3B|comment:The spliceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate B act complex 2mM ATP was added and a deleted mutant of actin mRNA was used to stall the splicing reaction.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2016/04/01 - rogid:0JoVp7ULlbib7N5+GOPksHStSmY559292 - false mutation:284-1437|tag:1-1|32p radiolabel:?-? - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:Q00539 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-11835|ensemblfungi:YHR086W|uniprotkb:D3DL37 psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:nam8_yeast(display_long)|uniprotkb:NAM8(gene name)|psi-mi:NAM8(display_short)|uniprotkb:MRE2(gene name synonym)|uniprotkb:YHR086W(locus name) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616300|imex:IM-25038-2 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - ensemblfungi:YHR086W(gene)|ensemblfungi:YHR086W_mRNA(transcript)|go:"GO:0000243"(commitment complex)|go:"GO:0000398"(mRNA splicing, via spliceosome)|go:"GO:0003729"(mRNA binding)|go:"GO:0005634"(nucleus)|go:"GO:0005737"(cytoplasm)|go:"GO:0005739"(mitochondrion)|go:"GO:0005829"(cytosol)|go:"GO:0006376"(mRNA splice site selection)|go:"GO:0048026"(positive regulation of mRNA splicing, via spliceosome)|go:"GO:0071004"(U2-type prespliceosome)|interpro:IPR000504(RNA recognition motif, RNP-1)|interpro:IPR012677(Nucleotide-binding, alpha-beta plait)|interpro:IPR035979|rcsb pdb:5ZWN|rcsb pdb:6G90|rcsb pdb:6N7P|rcsb pdb:6N7R|rcsb pdb:6N7X|go:"GO:0005685"(U1 snRNP)|refseq:NP_011954.1|sgd:S000001128|dip:DIP-2749N|mint:Q00539 - - crc64:64F198EEFB32A909 figure legend:Table 1, Suppl. Fig 3B|comment:The spliceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate B act complex 2mM ATP was added and a deleted mutant of actin mRNA was used to stall the splicing reaction.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2016/04/01 - rogid:WMVF9Z4Ro2MK2beb2IJy/CZ3fmA559292 - false mutation:284-1437|tag:1-1|32p radiolabel:?-? - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:P32639 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-861|uniprotkb:D3DM80|ensemblfungi:YER172C psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:brr2_yeast(display_long)|uniprotkb:BRR2(gene name)|psi-mi:BRR2(display_short)|uniprotkb:RSS1(gene name synonym)|uniprotkb:SNU246(gene name synonym)|uniprotkb:YER172C(locus name)|uniprotkb:SYGP-ORF66(orf name)|uniprotkb:Protein Snu246(gene name synonym) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616300|imex:IM-25038-2 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - refseq:NP_011099.1|sgd:S000000974|dip:DIP-850N|mint:P32639|ensemblfungi:YER172C(gene)|ensemblfungi:YER172C_mRNA(transcript)|go:"GO:0000388"("spliceosome conformational change to release U4 (or U4atac) and U1 (or U11)")|go:"GO:0003723"(RNA binding)|go:"GO:0003724"(RNA helicase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005634"(nucleus)|go:"GO:0005681"(spliceosomal complex)|go:"GO:0005682"(U5 snRNP)|go:"GO:0042802"(identical protein binding)|go:"GO:0046540"(U4/U6 x U5 tri-snRNP complex)|go:"GO:0140603"(obsolete ATP hydrolysis activity)|interpro:IPR001650(DNA/RNA helicase, C-terminal)|interpro:IPR004179(Sec63)|interpro:IPR011545(DNA/RNA helicase, DEAD/DEAH box type, N-terminal)|interpro:IPR014001(DEAD-like helicase, N-terminal)|interpro:IPR014756(Immunoglobulin E-set)|interpro:IPR027417|interpro:IPR035892|interpro:IPR036388|interpro:IPR036390|interpro:IPR041094|rcsb pdb:3HIB|rcsb pdb:3IM1|rcsb pdb:3IM2|rcsb pdb:3JCM|rcsb pdb:4BGD|rcsb pdb:5DCA|rcsb pdb:5GAN|rcsb pdb:5GAO|rcsb pdb:5GAP|rcsb pdb:5GM6|rcsb pdb:5LJ5|rcsb pdb:5LQW|rcsb pdb:5M52|rcsb pdb:5M5P|rcsb pdb:5NRL|rcsb pdb:5ZWM|rcsb pdb:5ZWO|rcsb pdb:7B9V - - crc64:DFAF7E3B7168D944 figure legend:Table 1, Suppl. Fig 3B|comment:The spliceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate B act complex 2mM ATP was added and a deleted mutant of actin mRNA was used to stall the splicing reaction.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2016/04/01 - rogid:2bNoVkou3QatkNCtPIiSdvPdgEo559292 - false mutation:284-1437|tag:1-1|32p radiolabel:?-? - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:P40018 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-432|uniprotkb:D3DLS8|ensemblfungi:YER029C psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:rsmb_yeast(display_long)|uniprotkb:SMB1(gene name)|psi-mi:SMB1(display_short)|uniprotkb:Sm protein B(gene name synonym)|uniprotkb:YER029C(locus name) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616300|imex:IM-25038-2 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - sgd:S000000831|rcsb pdb:5MPS|rcsb pdb:5MQ0|rcsb pdb:5NRL|rcsb pdb:5WSG|rcsb pdb:5Y88|rcsb pdb:5YLZ|rcsb pdb:5ZWM|rcsb pdb:5ZWN|rcsb pdb:5ZWO|rcsb pdb:6BK8|rcsb pdb:6EXN|rcsb pdb:6G90|rcsb pdb:6J6G|rcsb pdb:6J6H|rcsb pdb:6J6N|rcsb pdb:6J6Q|rcsb pdb:6N7P|rcsb pdb:6N7R|rcsb pdb:6N7X|rcsb pdb:7B9V|ensemblfungi:YER029C(gene)|ensemblfungi:YER029C_mRNA(transcript)|go:"GO:0000398"(mRNA splicing, via spliceosome)|go:"GO:0003723"(RNA binding)|go:"GO:0005682"(U5 snRNP)|go:"GO:0005685"(U1 snRNP)|go:"GO:0005686"(U2 snRNP)|go:"GO:0005687"(U4 snRNP)|go:"GO:0005737"(cytoplasm)|go:"GO:0046540"(U4/U6 x U5 tri-snRNP complex)|go:"GO:0071004"(U2-type prespliceosome)|go:"GO:0071013"(catalytic step 2 spliceosome)|interpro:IPR001163(Like-Sm ribonucleoprotein, core)|interpro:IPR010920(Like-Sm ribonucleoprotein-related, core)|rcsb pdb:3JCM|rcsb pdb:5GAM|rcsb pdb:5GAN|rcsb pdb:5GAO|rcsb pdb:5GM6|refseq:NP_010946.3|rcsb pdb:5GMK|rcsb pdb:5LJ3|rcsb pdb:5LJ5|rcsb pdb:5LQW|dip:DIP-843N|mint:P40018 - - crc64:0A1078D5AA241430 figure legend:Table 1, Suppl. Fig 3B|comment:The spliceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate B act complex 2mM ATP was added and a deleted mutant of actin mRNA was used to stall the splicing reaction.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2016/04/01 - rogid:b62+dBOdvQgeE0oup4kbXbviS1U559292 - false mutation:284-1437|tag:1-1|32p radiolabel:?-? - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:P25337 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-547|ensemblfungi:YCR063W|uniprotkb:D6VR67 psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:bud31_yeast(display_long)|uniprotkb:BUD31(gene name)|psi-mi:BUD31(display_short)|uniprotkb:Bud site selection protein 31(gene name synonym)|uniprotkb:CWC14(gene name synonym)|uniprotkb:Complexed with CEF1 protein 14(gene name synonym)|uniprotkb:YCR63W(orf name)|uniprotkb:YCR903(orf name)|uniprotkb:YCR063W(locus name) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616300|imex:IM-25038-2 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - refseq:NP_009990.1|sgd:S000000659|go:"GO:0000398"(mRNA splicing, via spliceosome)|go:"GO:0005634"(nucleus)|go:"GO:0005681"(spliceosomal complex)|go:"GO:0005686"(U2 snRNP)|interpro:IPR001748(G10 protein)|interpro:IPR018230|rcsb pdb:2MY1|ensemblfungi:YCR063W(gene)|ensemblfungi:YCR063W_mRNA(transcript)|go:"GO:0000282"(cellular bud site selection)|rcsb pdb:5GM6|rcsb pdb:5GMK|rcsb pdb:5LJ3|rcsb pdb:5LJ5|rcsb pdb:5LQW|rcsb pdb:5MPS|rcsb pdb:5MQ0|rcsb pdb:5WSG|rcsb pdb:5Y88|rcsb pdb:5YLZ|rcsb pdb:6BK8|rcsb pdb:6EXN|rcsb pdb:6J6G|rcsb pdb:6J6H|rcsb pdb:6J6N|rcsb pdb:6J6Q|dip:DIP-1180N|mint:P25337 - - crc64:70A8B15F8DA6F85D figure legend:Table 1, Suppl. Fig 3B|comment:The spliceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate B act complex 2mM ATP was added and a deleted mutant of actin mRNA was used to stall the splicing reaction.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2016/04/01 - rogid:DImrf7jgUrSuQtfiOvBeJr5Cl/w559292 - false mutation:284-1437|tag:1-1|32p radiolabel:?-? - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:P38241 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-780|uniprotkb:P89497|uniprotkb:D6VQ64|ensemblfungi:YBR065C psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:slt11_yeast(display_long)|uniprotkb:ECM2(gene name)|psi-mi:ECM2(display_short)|uniprotkb:SLT11(gene name synonym)|uniprotkb:YBR065C(locus name)|uniprotkb:YBR0614(orf name)|uniprotkb:Synthetic lethality with U2 protein 11(gene name synonym)|uniprotkb:Extracellular mutant protein 2(gene name synonym) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616300|imex:IM-25038-2 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - sgd:S000000269|refseq:NP_009621.3|dip:DIP-5359N|mint:P38241|ensemblfungi:YBR065C(gene)|ensemblfungi:YBR065C_mRNA(transcript)|go:"GO:0000398"(mRNA splicing, via spliceosome)|go:"GO:0000974"(Prp19 complex)|go:"GO:0005634"(nucleus)|go:"GO:0017070"(U6 snRNA binding)|go:"GO:0036002"(pre-mRNA binding)|go:"GO:0071006"(U2-type catalytic step 1 spliceosome)|go:"GO:0071007"(U2-type catalytic step 2 spliceosome)|interpro:IPR034356|interpro:IPR039171|rcsb pdb:5GM6|rcsb pdb:5GMK|rcsb pdb:5LJ3|rcsb pdb:5LJ5|rcsb pdb:5LQW|rcsb pdb:5MPS|rcsb pdb:5MQ0|rcsb pdb:5WSG|rcsb pdb:5Y88|rcsb pdb:5YLZ|rcsb pdb:6BK8|rcsb pdb:6EXN|rcsb pdb:6J6G|rcsb pdb:6J6H|rcsb pdb:6J6N|rcsb pdb:6J6Q - - crc64:BF21E687817A42B4 figure legend:Table 1, Suppl. Fig 3B|comment:The spliceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate B act complex 2mM ATP was added and a deleted mutant of actin mRNA was used to stall the splicing reaction.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2016/04/01 - rogid:ioGlVp9msutMpWXME0tICQn8GDQ559292 - false mutation:284-1437|tag:1-1|32p radiolabel:?-? - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:P28004 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-20640|uniprotkb:D6VPI6|ensemblfungi:YAL032C psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:prp45_yeast(display_long)|uniprotkb:YAL032C(locus name)|uniprotkb:PRP45(gene name)|psi-mi:PRP45(display_short)|uniprotkb:FUN20(gene name synonym) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616300|imex:IM-25038-2 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - refseq:NP_009370.1|sgd:S000000030|ensemblfungi:YAL032C(gene)|ensemblfungi:YAL032C_mRNA(transcript)|go:"GO:0000350"(generation of catalytic spliceosome for second transesterification step)|go:"GO:0005634"(nucleus)|go:"GO:0071006"(U2-type catalytic step 1 spliceosome)|go:"GO:0071007"(U2-type catalytic step 2 spliceosome)|go:"GO:0071014"(post-mRNA release spliceosomal complex)|interpro:IPR004015(SKIP/SNW)|interpro:IPR017862|rcsb pdb:5GM6|rcsb pdb:5GMK|rcsb pdb:5LJ3|rcsb pdb:5LJ5|rcsb pdb:5LQW|rcsb pdb:5MPS|rcsb pdb:5MQ0|rcsb pdb:5WSG|rcsb pdb:5Y88|rcsb pdb:5YLZ|rcsb pdb:6BK8|rcsb pdb:6EXN|rcsb pdb:6J6G|rcsb pdb:6J6H|rcsb pdb:6J6N|rcsb pdb:6J6Q|dip:DIP-2774N|mint:P28004 - - crc64:FE5A724186D7FE84 figure legend:Table 1, Suppl. Fig 3B|comment:The spliceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate B act complex 2mM ATP was added and a deleted mutant of actin mRNA was used to stall the splicing reaction.|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2016/04/01 - rogid:qoHF1UsO3k+JOTOfigSuy2P84/k559292 - false mutation:284-1437|tag:1-1|32p radiolabel:?-? - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:P0C074 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-970846|uniprotkb:D6W143|ensemblfungi:YNL138W-A psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:ysf3_yeast(display_long)|uniprotkb:YSF3(gene name)|psi-mi:YSF3(display_short)|uniprotkb:RCP10(gene name synonym)|uniprotkb:YNL138W-A(locus name)|uniprotkb:Splicing factor 3b subunit(gene name synonym) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616364|imex:IM-25038-3 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - go:"GO:0000398"(mRNA splicing, via spliceosome)|go:"GO:0005686"(U2 snRNP)|go:"GO:0071011"(precatalytic spliceosome)|ensemblfungi:YNL138W-A(gene)|ensemblfungi:YNL138W-A_mRNA(transcript)|go:"GO:0000245"(spliceosomal complex assembly)|refseq:NP_878153.1|sgd:S000028509|interpro:IPR009846(Splicing factor 3B subunit 5/RDS3 complex subunit 10)|rcsb pdb:7DCO|rcsb pdb:5GM6|rcsb pdb:5LQW|rcsb pdb:5ZWM|rcsb pdb:5ZWO|rcsb pdb:6G90|rcsb pdb:5NRL - - - figure legend:Table 1, Suppl Fig4|comment:The spiceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate C complex 2mM ATP was added and a deletion mutant of actin premRNA was used|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2017/12/02 - rogid:+ifBAgC9wh9hksXMFWXrcwu8eOU559292 - false 32p radiolabel:?-?|tag:1-1|mutation:1-308 - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 intact:EBI-10860871 ensembl:YFL039C.1|sgd:YFL039C rnacentral:URS000023C730_559292 psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:lsr1_snr20_u2 yeast(display_short)|psi-mi:EBI-10860871(display_long) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616364|imex:IM-25038-3 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0607"(small nuclear rna) - - - - - figure legend:Table 1, Suppl Fig4|comment:The spiceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate C complex 2mM ATP was added and a deletion mutant of actin premRNA was used|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2017/12/02 - - - false 32p radiolabel:?-?|tag:1-1|mutation:1-308 - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0929"(northern blot) intact:EBI-11413715 intact:EBI-10825220 ensembl:YFL039C.1|sgd:YFL039C rnacentral:URS0000514318_559292 psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:snr7s_u5_yeast(display_short)|psi-mi:EBI-10825220(display_long) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616364|imex:IM-25038-3 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0607"(small nuclear rna) - - - - - figure legend:Table 1, Suppl Fig4|comment:The spiceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate C complex 2mM ATP was added and a deletion mutant of actin premRNA was used|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2017/12/02 - - - false 32p radiolabel:?-?|tag:1-1|mutation:1-308 - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0929"(northern blot) intact:EBI-11413715 uniprotkb:P54999 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-770|uniprotkb:D6W4I2|ensemblfungi:YPR182W psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:ruxf_yeast(display_long)|uniprotkb:SMX3(gene name)|psi-mi:SMX3(display_short)|uniprotkb:YPR182W(locus name)|uniprotkb:P9705.4(orf name)|uniprotkb:Sm protein F(gene name synonym) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616364|imex:IM-25038-3 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - refseq:NP_015508.1|sgd:S000006386|dip:DIP-1634N|mint:P54999|ensemblfungi:YPR182W(gene)|ensemblfungi:YPR182W_mRNA(transcript)|go:"GO:0000387"(spliceosomal snRNP assembly)|go:"GO:0000398"(mRNA splicing, via spliceosome)|go:"GO:0003723"(RNA binding)|go:"GO:0005682"(U5 snRNP)|go:"GO:0005685"(U1 snRNP)|go:"GO:0005686"(U2 snRNP)|go:"GO:0008266"("poly(U) RNA binding")|go:"GO:0046540"(U4/U6 x U5 tri-snRNP complex)|go:"GO:0071013"(catalytic step 2 spliceosome)|go:"GO:1990935"(splicing factor binding)|interpro:IPR001163(Like-Sm ribonucleoprotein, core)|interpro:IPR010920(Like-Sm ribonucleoprotein-related, core)|interpro:IPR016487(Small nuclear ribonucleoprotein SmF)|interpro:IPR034100|rcsb pdb:1N9R|rcsb pdb:1N9S|rcsb pdb:3JCM|rcsb pdb:5GAM|rcsb pdb:5GAN|rcsb pdb:5GAO|rcsb pdb:5GM6|rcsb pdb:5GMK|rcsb pdb:5LJ3|rcsb pdb:5LJ5|rcsb pdb:5LQW|rcsb pdb:5MPS|rcsb pdb:5MQ0|rcsb pdb:5NRL|rcsb pdb:5WSG|rcsb pdb:5Y88|rcsb pdb:5YLZ|rcsb pdb:5ZWM|rcsb pdb:5ZWN|rcsb pdb:5ZWO|rcsb pdb:6BK8|rcsb pdb:6EXN|rcsb pdb:6G90|rcsb pdb:6J6G|rcsb pdb:6J6H|rcsb pdb:6J6N|rcsb pdb:6J6Q|rcsb pdb:6N7P|rcsb pdb:6N7R|rcsb pdb:6N7X|rcsb pdb:7B9V - - crc64:1640D3237597B2D8 figure legend:Table 1, Suppl Fig4|comment:The spiceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate C complex 2mM ATP was added and a deletion mutant of actin premRNA was used|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2017/12/02 - rogid:iyBbVNf2fgOIqhnOg9Etn3P2r3Q559292 - false 32p radiolabel:?-?|tag:1-1|mutation:1-308 - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:Q06091 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-818|uniprotkb:D6W499|ensemblfungi:YPR101W psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:sn309_yeast(display_long)|uniprotkb:YPR101W(locus name)|uniprotkb:SNT309(gene name)|psi-mi:SNT309(display_short)|uniprotkb:NTC25(gene name synonym)|uniprotkb:Synergistic to PRP19 mutation protein 309(gene name synonym)|uniprotkb:PRP19-associated complex protein 25(gene name synonym) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616364|imex:IM-25038-3 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - sgd:S000006305|refseq:NP_015426.1|dip:DIP-2864N|mint:Q06091|ensemblfungi:YPR101W(gene)|ensemblfungi:YPR101W_mRNA(transcript)|go:"GO:0000398"(mRNA splicing, via spliceosome)|go:"GO:0000974"(Prp19 complex)|go:"GO:0071006"(U2-type catalytic step 1 spliceosome)|rcsb pdb:5GM6|rcsb pdb:5GMK|rcsb pdb:5LJ5|rcsb pdb:5MQ0|rcsb pdb:5WSG|rcsb pdb:5Y88|rcsb pdb:5YLZ|rcsb pdb:6BK8|rcsb pdb:6EXN|rcsb pdb:6J6G|rcsb pdb:6J6H|rcsb pdb:6J6N|rcsb pdb:6J6Q - - crc64:B781CA65BC9A1918 figure legend:Table 1, Suppl Fig4|comment:The spiceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate C complex 2mM ATP was added and a deletion mutant of actin premRNA was used|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2017/12/02 - rogid:ELyyYlQDmNRzWKQx/63xvHuAG8M559292 - false 32p radiolabel:?-?|tag:1-1|mutation:1-308 - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:Q08963 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-594|uniprotkb:D6W3F7|ensemblfungi:YPL213W psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:ru2a_yeast(display_long)|uniprotkb:YPL213W(locus name)|uniprotkb:LEA1(gene name)|psi-mi:LEA1(display_short)|uniprotkb:Looks exceptionally like U2A protein 1(gene name synonym) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616364|imex:IM-25038-3 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - refseq:NP_015111.1|sgd:S000006134|dip:DIP-2399N|mint:Q08963|ensemblfungi:YPL213W(gene)|ensemblfungi:YPL213W_mRNA(transcript)|go:"GO:0000398"(mRNA splicing, via spliceosome)|go:"GO:0005686"(U2 snRNP)|go:"GO:0005737"(cytoplasm)|go:"GO:0030620"(U2 snRNA binding)|go:"GO:0071004"(U2-type prespliceosome)|interpro:IPR001611(Leucine-rich repeat)|interpro:IPR003603(U2A'/phosphoprotein 32 family A, C-terminal)|interpro:IPR032675|interpro:IPR044640|rcsb pdb:5GMK|rcsb pdb:5LJ3|rcsb pdb:5LJ5|rcsb pdb:5MQ0|rcsb pdb:5NRL|rcsb pdb:5WSG|rcsb pdb:5Y88|rcsb pdb:5YLZ|rcsb pdb:5ZWM|rcsb pdb:5ZWO|rcsb pdb:6BK8|rcsb pdb:6EXN|rcsb pdb:6G90|rcsb pdb:6J6G|rcsb pdb:6J6H|rcsb pdb:6J6N|rcsb pdb:6J6Q - - - figure legend:Table 1, Suppl Fig4|comment:The spiceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate C complex 2mM ATP was added and a deletion mutant of actin premRNA was used|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2017/12/02 - rogid:PlKwD+gRYbhUBE4GcxTaP0wt8zM559292 - false 32p radiolabel:?-?|tag:1-1|mutation:1-308 - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:Q12417 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-710|uniprotkb:D6W3L8|ensemblfungi:YPL151C psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:prp46_yeast(display_long)|uniprotkb:CWC1(gene name synonym)|uniprotkb:PRP nineteen-associated complex protein 50(gene name synonym)|uniprotkb:PRP19-associated complex protein 50(gene name synonym)|uniprotkb:P2594(orf name)|uniprotkb:YPL151C(locus name)|uniprotkb:PRP46(gene name)|psi-mi:PRP46(display_short)|uniprotkb:NTC50(gene name synonym)|uniprotkb:Pre-mRNA-processing protein 46(gene name synonym)|uniprotkb:Complexed with CEF1 protein 1(gene name synonym) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616364|imex:IM-25038-3 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - refseq:NP_015174.1|sgd:S000006072|ensemblfungi:YPL151C(gene)|ensemblfungi:YPL151C_mRNA(transcript)|go:"GO:0000398"(mRNA splicing, via spliceosome)|go:"GO:0000974"(Prp19 complex)|go:"GO:0005681"(spliceosomal complex)|go:"GO:0005737"(cytoplasm)|go:"GO:0071013"(catalytic step 2 spliceosome)|go:"GO:0080008"(Cul4-RING E3 ubiquitin ligase complex)|interpro:IPR001680(WD40 repeat)|interpro:IPR015943(WD40/YVTN repeat-like)|interpro:IPR019775|interpro:IPR020472|interpro:IPR036322|dip:DIP-3994N|mint:Q12417|rcsb pdb:5GM6|rcsb pdb:5GMK|rcsb pdb:5LJ3|rcsb pdb:5LJ5|rcsb pdb:5LQW|rcsb pdb:5MPS|rcsb pdb:5MQ0|rcsb pdb:5WSG|rcsb pdb:5Y88|rcsb pdb:5YLZ|rcsb pdb:6BK8|rcsb pdb:6EXN|rcsb pdb:6J6G|rcsb pdb:6J6H|rcsb pdb:6J6N|rcsb pdb:6J6Q - - crc64:0E888F566A5414BD figure legend:Table 1, Suppl Fig4|comment:The spiceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate C complex 2mM ATP was added and a deletion mutant of actin premRNA was used|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2017/12/02 - rogid:016ynVER9V3d44NwkqRG7TIvf9w559292 - false 32p radiolabel:?-?|tag:1-1|mutation:1-308 - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:Q02770 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-35484|uniprotkb:D6W3V1|ensemblfungi:YPL064C psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:cwc27_yeast(display_long)|uniprotkb:CWC27(gene name)|psi-mi:CWC27(display_short)|uniprotkb:Complexed with CEF1 protein 27(gene name synonym)|uniprotkb:Rotamase CWC27(gene name synonym)|uniprotkb:CYP7(gene name synonym)|uniprotkb:YPL064C(locus name) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616364|imex:IM-25038-3 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - refseq:NP_015261.1|sgd:S000005985|dip:DIP-2778N|ensemblfungi:YPL064C(gene)|ensemblfungi:YPL064C_mRNA(transcript)|go:"GO:0000413"(protein peptidyl-prolyl isomerization)|go:"GO:0003755"(peptidyl-prolyl cis-trans isomerase activity)|go:"GO:0005684"(U2-type spliceosomal complex)|go:"GO:0005737"(cytoplasm)|go:"GO:0006397"(mRNA processing)|go:"GO:0008380"(RNA splicing)|interpro:IPR002130(Peptidyl-prolyl cis-trans isomerase, cyclophilin-type)|interpro:IPR029000|interpro:IPR044666|rcsb pdb:5GM6 - - - figure legend:Table 1, Suppl Fig4|comment:The spiceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate C complex 2mM ATP was added and a deletion mutant of actin premRNA was used|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2017/12/02 - rogid:evoBtq7Y4Qzpk0toZEmGq1ECihM559292 - false 32p radiolabel:?-?|tag:1-1|mutation:1-308 - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:Q99181 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-8579|uniprotkb:D6W317|ensemblfungi:YOR319W psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:hsh49_yeast(display_long)|uniprotkb:O6142(orf name)|uniprotkb:YOR319W(locus name)|uniprotkb:HSH49(gene name)|psi-mi:HSH49(display_short) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616364|imex:IM-25038-3 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - refseq:NP_014964.1|sgd:S000005846|mint:Q99181|dip:DIP-930N|ensemblfungi:YOR319W(gene)|ensemblfungi:YOR319W_mRNA(transcript)|go:"GO:0000398"(mRNA splicing, via spliceosome)|go:"GO:0003723"(RNA binding)|go:"GO:0003730"(mRNA 3'-UTR binding)|go:"GO:0005634"(nucleus)|go:"GO:0005686"(U2 snRNP)|go:"GO:0005829"(cytosol)|go:"GO:0008143"("poly(A) binding")|go:"GO:0008266"("poly(U) RNA binding")|go:"GO:0071004"(U2-type prespliceosome)|go:"GO:1990904"(ribonucleoprotein complex)|interpro:IPR000504(RNA recognition motif, RNP-1)|interpro:IPR012677(Nucleotide-binding, alpha-beta plait)|interpro:IPR034158|interpro:IPR035979|rcsb pdb:5GM6|rcsb pdb:5LSB|rcsb pdb:5LSL|rcsb pdb:5NRL|rcsb pdb:5ZWM|rcsb pdb:5ZWO|rcsb pdb:6G90 - - crc64:F6FC44083094B176 figure legend:Table 1, Suppl Fig4|comment:The spiceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate C complex 2mM ATP was added and a deletion mutant of actin premRNA was used|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2017/12/02 - rogid:LWcxvibHcl0Oyi1OidqUtMnOLP8559292 - false 32p radiolabel:?-?|tag:1-1|mutation:1-308 - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:Q12330 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-16359|uniprotkb:D6W2L6|ensemblfungi:YOR159C psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:ruxe_yeast(display_long)|uniprotkb:SME1(gene name)|psi-mi:SME1(display_short)|uniprotkb:YOR159C(locus name)|uniprotkb:Sm protein E(gene name synonym) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616364|imex:IM-25038-3 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - ensemblfungi:YOR159C(gene)|ensemblfungi:YOR159C_mRNA(transcript)|go:"GO:0000387"(spliceosomal snRNP assembly)|go:"GO:0000398"(mRNA splicing, via spliceosome)|go:"GO:0003723"(RNA binding)|go:"GO:0005682"(U5 snRNP)|go:"GO:0005685"(U1 snRNP)|go:"GO:0005686"(U2 snRNP)|go:"GO:0005687"(U4 snRNP)|go:"GO:0005737"(cytoplasm)|go:"GO:0046540"(U4/U6 x U5 tri-snRNP complex)|go:"GO:0071004"(U2-type prespliceosome)|go:"GO:0071011"(precatalytic spliceosome)|go:"GO:1990935"(splicing factor binding)|interpro:IPR001163(Like-Sm ribonucleoprotein, core)|interpro:IPR010920(Like-Sm ribonucleoprotein-related, core)|interpro:IPR027078|rcsb pdb:3JCM|rcsb pdb:5GAM|rcsb pdb:5GAN|rcsb pdb:5GAO|rcsb pdb:5GM6|rcsb pdb:5GMK|sgd:S000005685|rcsb pdb:5LJ3|rcsb pdb:5LJ5|rcsb pdb:5LQW|rcsb pdb:5MPS|rcsb pdb:5MQ0|rcsb pdb:5NRL|rcsb pdb:5WSG|rcsb pdb:5Y88|rcsb pdb:5YLZ|rcsb pdb:5ZWM|rcsb pdb:5ZWN|rcsb pdb:5ZWO|rcsb pdb:6BK8|rcsb pdb:6EXN|rcsb pdb:6G90|rcsb pdb:6J6G|rcsb pdb:6J6H|rcsb pdb:6J6N|rcsb pdb:6J6Q|rcsb pdb:6N7P|rcsb pdb:6N7R|rcsb pdb:6N7X|rcsb pdb:7B9V|refseq:NP_014802.3|dip:DIP-1633N|mint:Q12330 - - crc64:B76D72DA8456F3B5 figure legend:Table 1, Suppl Fig4|comment:The spiceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate C complex 2mM ATP was added and a deletion mutant of actin premRNA was used|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2017/12/02 - rogid:IiV/AXz4zJjC7A2iuBixxiLYo9E559292 - false 32p radiolabel:?-?|tag:1-1|mutation:1-308 - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:Q02521 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-17866|uniprotkb:Q12353|uniprotkb:D6W2K5|ensemblfungi:YOR148C psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:spp2_yeast(display_long)|uniprotkb:SPP2(gene name)|psi-mi:SPP2(display_short)|uniprotkb:Spliceosome maturation protein SPP2(gene name synonym)|uniprotkb:Suppressor of PRP protein 2(gene name synonym)|uniprotkb:YOR148C(locus name) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616364|imex:IM-25038-3 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - refseq:NP_014791.1|sgd:S000005674|go:"GO:0000398"(mRNA splicing, via spliceosome)|go:"GO:0001671"(ATPase activator activity)|go:"GO:0003676"(nucleic acid binding)|go:"GO:0005681"(spliceosomal complex)|go:"GO:0005737"(cytoplasm)|go:"GO:0032781"(positive regulation of ATP-dependent activity)|interpro:IPR000467(D111/G-patch)|interpro:IPR026822|rcsb pdb:7DCO|rcsb pdb:7DCP|rcsb pdb:7DCR|rcsb pdb:7DD3|ensemblfungi:YOR148C(gene)|ensemblfungi:YOR148C_mRNA(transcript)|dip:DIP-4140N|mint:Q02521 - - - figure legend:Table 1, Suppl Fig4|comment:The spiceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate C complex 2mM ATP was added and a deletion mutant of actin premRNA was used|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2017/12/02 - rogid:dKji+Gn2/e+tF0W2O37A2HeG4Cc559292 - false 32p radiolabel:?-?|tag:1-1|mutation:1-308 - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:P20095 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-13820|ensemblfungi:YNR011C|uniprotkb:D6W1I6 psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:prp2_yeast(display_long)|uniprotkb:PRP2(gene name)|psi-mi:PRP2(display_short)|uniprotkb:RNA2(gene name synonym)|uniprotkb:YNR011C(locus name)|uniprotkb:N2048(orf name)|uniprotkb:Pre-mRNA-processing protein 2(gene name synonym) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616364|imex:IM-25038-3 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - refseq:NP_014408.1|sgd:S000005294|interpro:IPR001650(DNA/RNA helicase, C-terminal)|interpro:IPR002464(DNA/RNA helicase, ATP-dependent, DEAH-box type, conserved site)|interpro:IPR007502(Helicase-associated region)|interpro:IPR011545(DNA/RNA helicase, DEAD/DEAH box type, N-terminal)|interpro:IPR011709(Region of unknown function DUF1605)|interpro:IPR014001(DEAD-like helicase, N-terminal)|interpro:IPR027417|rcsb pdb:5GM6|rcsb pdb:5LQW|rcsb pdb:7DCO|rcsb pdb:7DCP|rcsb pdb:7DCQ|rcsb pdb:7DCR|rcsb pdb:7DD3|go:"GO:0140603"(obsolete ATP hydrolysis activity)|ensemblfungi:YNR011C(gene)|ensemblfungi:YNR011C_mRNA(transcript)|go:"GO:0000349"(generation of catalytic spliceosome for first transesterification step)|go:"GO:0003723"(RNA binding)|go:"GO:0003724"(RNA helicase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0008186"(ATP-dependent activity, acting on RNA)|go:"GO:0034247"(snoRNA splicing)|go:"GO:0071006"(U2-type catalytic step 1 spliceosome)|mint:P20095|dip:DIP-3961N - - crc64:7EB3E1EE93215F57 figure legend:Table 1, Suppl Fig4|comment:The spiceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate C complex 2mM ATP was added and a deletion mutant of actin premRNA was used|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2017/12/02 - rogid:wfkc/eidpcEF1HlVabj2p/arPiY559292 - false 32p radiolabel:?-?|tag:1-1|mutation:1-308 - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:P53854 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-29221|uniprotkb:D6W0U8|ensemblfungi:YNL245C psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:cwc25_yeast(display_long)|uniprotkb:Complexed with CEF1 protein 25(gene name synonym)|uniprotkb:YNL245C(locus name)|uniprotkb:N0901(orf name)|uniprotkb:CWC25(gene name)|psi-mi:CWC25(display_short) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616364|imex:IM-25038-3 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - refseq:NP_014154.1|sgd:S000005189|dip:DIP-2781N|ensemblfungi:YNL245C(gene)|ensemblfungi:YNL245C_mRNA(transcript)|go:"GO:0000398"(mRNA splicing, via spliceosome)|go:"GO:0005684"(U2-type spliceosomal complex)|interpro:IPR019339|interpro:IPR022209|rcsb pdb:5GMK|rcsb pdb:5LJ3|rcsb pdb:5LJ5 - - - figure legend:Table 1, Suppl Fig4|comment:The spiceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate C complex 2mM ATP was added and a deletion mutant of actin premRNA was used|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2017/12/02 - rogid:6bRgoCHPWEJzKP26V+7zR6CtCMY559292 - false 32p radiolabel:?-?|tag:1-1|mutation:1-308 - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:P49955 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-664|uniprotkb:D6W0B5|ensemblfungi:YMR288W psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:sf3b1_yeast(display_long)|uniprotkb:YMR288W(locus name)|uniprotkb:YM8021.14(orf name)|uniprotkb:HSH155(gene name)|psi-mi:HSH155(display_short) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616364|imex:IM-25038-3 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - refseq:NP_014015.1|sgd:S000004901|dip:DIP-2628N|mint:P49955|ensemblfungi:YMR288W(gene)|ensemblfungi:YMR288W_mRNA(transcript)|go:"GO:0000245"(spliceosomal complex assembly)|go:"GO:0000398"(mRNA splicing, via spliceosome)|go:"GO:0003729"(mRNA binding)|go:"GO:0005686"(U2 snRNP)|go:"GO:0071004"(U2-type prespliceosome)|go:"GO:0071013"(catalytic step 2 spliceosome)|interpro:IPR011989(Armadillo-like helical)|interpro:IPR016024(Armadillo-type fold)|interpro:IPR038737|rcsb pdb:5GM6|rcsb pdb:5LQW|rcsb pdb:5NRL|rcsb pdb:5ZWM|rcsb pdb:5ZWO|rcsb pdb:6G90 - - crc64:27D26E4252A788E2 figure legend:Table 1, Suppl Fig4|comment:The spiceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate C complex 2mM ATP was added and a deletion mutant of actin premRNA was used|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2017/12/02 - rogid:t0fwQOOum/AO/xL8vhZdF2EGymo559292 - false 32p radiolabel:?-?|tag:1-1|mutation:1-308 - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:Q02554 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-654|uniprotkb:Q04012|uniprotkb:D6W066|ensemblfungi:YMR240C psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:cus1_yeast(display_long)|uniprotkb:YM9408.02C(orf name)|uniprotkb:YMR240C(locus name)|uniprotkb:CUS1(gene name)|psi-mi:CUS1(display_short) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616364|imex:IM-25038-3 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - refseq:NP_013967.1|sgd:S000004853|dip:DIP-910N|mint:Q02554|ensemblfungi:YMR240C(gene)|ensemblfungi:YMR240C_mRNA(transcript)|go:"GO:0000245"(spliceosomal complex assembly)|go:"GO:0000398"(mRNA splicing, via spliceosome)|go:"GO:0003723"(RNA binding)|go:"GO:0005684"(U2-type spliceosomal complex)|go:"GO:0005686"(U2 snRNP)|go:"GO:0071004"(U2-type prespliceosome)|go:"GO:0071011"(precatalytic spliceosome)|go:"GO:0071013"(catalytic step 2 spliceosome)|interpro:IPR006568(PSP, proline-rich)|interpro:IPR007180(Protein of unknown function DUF382)|rcsb pdb:5GM6|rcsb pdb:5LSB|rcsb pdb:5LSL|rcsb pdb:5NRL|rcsb pdb:5ZWM|rcsb pdb:5ZWO|rcsb pdb:6G90 - - crc64:0204B3A666AF7336 figure legend:Table 1, Suppl Fig4|comment:The spiceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate C complex 2mM ATP was added and a deletion mutant of actin premRNA was used|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2017/12/02 - rogid:4LtSdsLH2EISJjPGM78b73TD0yw559292 - false 32p radiolabel:?-?|tag:1-1|mutation:1-308 - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:Q03654 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-476|uniprotkb:Q6B1D8|ensemblfungi:YMR213W|uniprotkb:D6W038 psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:cef1_yeast(display_long)|uniprotkb:CEF1(gene name)|psi-mi:CEF1(display_short)|uniprotkb:YMR213W(locus name)|uniprotkb:YM8261.07(orf name)|uniprotkb:NTC85(gene name synonym)|uniprotkb:PRP nineteen-associated complex protein 85(gene name synonym)|uniprotkb:PRP19-associated complex protein 85(gene name synonym) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616364|imex:IM-25038-3 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - refseq:NP_013940.1|sgd:S000004826|ensemblfungi:YMR213W(gene)|ensemblfungi:YMR213W_mRNA(transcript)|go:"GO:0000350"(generation of catalytic spliceosome for second transesterification step)|go:"GO:0000398"(mRNA splicing, via spliceosome)|go:"GO:0000974"(Prp19 complex)|go:"GO:0003677"(DNA binding)|go:"GO:0005681"(spliceosomal complex)|go:"GO:0005737"(cytoplasm)|go:"GO:0071006"(U2-type catalytic step 1 spliceosome)|interpro:IPR001005(SANT, DNA-binding)|interpro:IPR009057(Homeodomain-like)|interpro:IPR017930|interpro:IPR021786|rcsb pdb:5GM6|rcsb pdb:5GMK|rcsb pdb:5LJ3|rcsb pdb:5LJ5|rcsb pdb:5LQW|rcsb pdb:5MPS|rcsb pdb:5MQ0|rcsb pdb:5WSG|rcsb pdb:5Y88|rcsb pdb:5YLZ|rcsb pdb:6BK8|rcsb pdb:6EXN|rcsb pdb:6J6G|rcsb pdb:6J6H|rcsb pdb:6J6N|rcsb pdb:6J6Q|rcsb pdb:7DCO|dip:DIP-1113N|mint:Q03654 - - crc64:A4C417A8B330EA9C figure legend:Table 1, Suppl Fig4|comment:The spiceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate C complex 2mM ATP was added and a deletion mutant of actin premRNA was used|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2017/12/02 - rogid:AsbV7RtNk+8mCzLk2wZYqbULOFg559292 - false 32p radiolabel:?-?|tag:1-1|mutation:1-308 - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:Q04693 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-519|ensemblfungi:YML049C|uniprotkb:D6VZC6 psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:rse1_yeast(display_long)|uniprotkb:RSE1(gene name)|psi-mi:RSE1(display_short)|uniprotkb:YML049C(locus name)|uniprotkb:YM9827.03C(orf name)|uniprotkb:SAP130(gene name synonym)|uniprotkb:RNA splicing and ER to Golgi transport factor 1(gene name synonym)|uniprotkb:Spliceosome-associated protein 130(gene name synonym) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616364|imex:IM-25038-3 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - refseq:NP_013663.1|sgd:S000004513|ensemblfungi:YML049C(gene)|ensemblfungi:YML049C_mRNA(transcript)|go:"GO:0000245"(spliceosomal complex assembly)|go:"GO:0000398"(mRNA splicing, via spliceosome)|go:"GO:0005634"(nucleus)|go:"GO:0005686"(U2 snRNP)|go:"GO:0030620"(U2 snRNA binding)|go:"GO:0071004"(U2-type prespliceosome)|interpro:IPR004871(Cleavage and polyadenylation specificity factor, A subunit, C-terminal)|interpro:IPR015943(WD40/YVTN repeat-like)|interpro:IPR018846|rcsb pdb:5GM6|rcsb pdb:5LQW|rcsb pdb:5NRL|rcsb pdb:5ZWM|rcsb pdb:5ZWO|rcsb pdb:6G90|dip:DIP-856N|mint:Q04693 - - crc64:4D92837F5C267D67 figure legend:Table 1, Suppl Fig4|comment:The spiceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate C complex 2mM ATP was added and a deletion mutant of actin premRNA was used|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2017/12/02 - rogid:L2J86R3nXgMd8CE/6Yd2jxA/sx4559292 - false 32p radiolabel:?-?|tag:1-1|mutation:1-308 - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:Q06411 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-576|uniprotkb:D6VZ60|ensemblfungi:YLR424W psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:sp382_yeast(display_long)|uniprotkb:NTR1(gene name synonym)|uniprotkb:YLR424W(locus name)|uniprotkb:SPP382(gene name)|psi-mi:SPP382(display_short)|uniprotkb:CCF8(gene name synonym)|uniprotkb:Suppressor of PRP38 protein 2(gene name synonym)|uniprotkb:Nineteen complex-related protein 1(gene name synonym)|uniprotkb:CLF1 complex factor 8(gene name synonym) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616364|imex:IM-25038-3 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - refseq:NP_013528.1|sgd:S000004416|dip:DIP-1491N|mint:Q06411|ensemblfungi:YLR424W(gene)|ensemblfungi:YLR424W_mRNA(transcript)|go:"GO:0000349"(generation of catalytic spliceosome for first transesterification step)|go:"GO:0000390"(spliceosomal complex disassembly)|go:"GO:0000781"(chromosome, telomeric region)|go:"GO:0003676"(nucleic acid binding)|go:"GO:0005634"(nucleus)|go:"GO:0005681"(spliceosomal complex)|go:"GO:0005730"(nucleolus)|go:"GO:0005737"(cytoplasm)|go:"GO:0005739"(mitochondrion)|go:"GO:0031333"(negative regulation of protein-containing complex assembly)|go:"GO:0032091"(negative regulation of protein binding)|go:"GO:0071008"(U2-type post-mRNA release spliceosomal complex)|go:"GO:1904876"(negative regulation of DNA ligase activity)|go:"GO:2001033"(negative regulation of double-strand break repair via nonhomologous end joining)|interpro:IPR000467(D111/G-patch)|rcsb pdb:5Y88 - - crc64:9986B547B9582338 figure legend:Table 1, Suppl Fig4|comment:The spiceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate C complex 2mM ATP was added and a deletion mutant of actin premRNA was used|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2017/12/02 - rogid:HrG54vTE2zs7jxPSzi+9eeA23/k559292 - false 32p radiolabel:?-?|tag:1-1|mutation:1-308 - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:P53769 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-27128|uniprotkb:D6VYW5|ensemblfungi:YLR323C psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:cwc24_yeast(display_long)|uniprotkb:YLR323C(locus name)|uniprotkb:L8543.3(orf name)|uniprotkb:Complexed with CEF1 protein 24(gene name synonym)|uniprotkb:CWC24(gene name)|psi-mi:CWC24(display_short) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616364|imex:IM-25038-3 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - refseq:NP_013427.1|sgd:S000004315|dip:DIP-1391N|ensemblfungi:YLR323C(gene)|ensemblfungi:YLR323C_mRNA(transcript)|go:"GO:0000349"(generation of catalytic spliceosome for first transesterification step)|go:"GO:0000398"(mRNA splicing, via spliceosome)|go:"GO:0003677"(DNA binding)|go:"GO:0005634"(nucleus)|go:"GO:0005681"(spliceosomal complex)|go:"GO:0005684"(U2-type spliceosomal complex)|go:"GO:0034247"(snoRNA splicing)|go:"GO:0046872"(metal ion binding)|interpro:IPR000571(Zinc finger, CCCH-type)|interpro:IPR001841(Zinc finger, RING-type)|interpro:IPR013083(Zinc finger, RING/FYVE/PHD-type)|interpro:IPR036855|interpro:IPR039971|rcsb pdb:5GM6 - - crc64:4371454CCB6F13D1 figure legend:Table 1, Suppl Fig4|comment:The spiceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate C complex 2mM ATP was added and a deletion mutant of actin premRNA was used|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2017/12/02 - rogid:wehPlmPZY5Cp2VSmkkqc3wZifig559292 - false 32p radiolabel:?-?|tag:1-1|mutation:1-308 - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:Q06217 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-235|uniprotkb:D6VYS2|ensemblfungi:YLR275W psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:smd2_yeast(display_long)|uniprotkb:SMD2(gene name)|psi-mi:SMD2(display_short)|uniprotkb:YLR275W(locus name)|uniprotkb:L9328.5(orf name)|uniprotkb:snRNP core protein D2(gene name synonym) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616364|imex:IM-25038-3 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - sgd:S000004265|refseq:NP_013377.1|mint:Q06217|dip:DIP-1421N|ensemblfungi:YLR275W(gene)|ensemblfungi:YLR275W_mRNA(transcript)|go:"GO:0000387"(spliceosomal snRNP assembly)|go:"GO:0003723"(RNA binding)|go:"GO:0005682"(U5 snRNP)|go:"GO:0005685"(U1 snRNP)|go:"GO:0005686"(U2 snRNP)|go:"GO:0046540"(U4/U6 x U5 tri-snRNP complex)|go:"GO:0071004"(U2-type prespliceosome)|go:"GO:0071011"(precatalytic spliceosome)|go:"GO:0071013"(catalytic step 2 spliceosome)|interpro:IPR001163(Like-Sm ribonucleoprotein, core)|interpro:IPR010920(Like-Sm ribonucleoprotein-related, core)|interpro:IPR027248|rcsb pdb:3JCM|rcsb pdb:5GAM|rcsb pdb:5GAN|rcsb pdb:5GAO|rcsb pdb:5GM6|rcsb pdb:5GMK|rcsb pdb:5LJ3|rcsb pdb:5LJ5|rcsb pdb:5LQW|rcsb pdb:5MPS|rcsb pdb:5MQ0|rcsb pdb:5NRL|rcsb pdb:5WSG|rcsb pdb:5Y88|rcsb pdb:5YLZ|rcsb pdb:5ZWM|rcsb pdb:5ZWN|rcsb pdb:5ZWO|rcsb pdb:6BK8|rcsb pdb:6EXN|rcsb pdb:6G90|rcsb pdb:6J6G|rcsb pdb:6J6H|rcsb pdb:6J6N|rcsb pdb:6J6Q|rcsb pdb:6N7P|rcsb pdb:6N7R|rcsb pdb:6N7X|rcsb pdb:7B9V - - crc64:7287070B10E090C0 figure legend:Table 1, Suppl Fig4|comment:The spiceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate C complex 2mM ATP was added and a deletion mutant of actin premRNA was used|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2017/12/02 - rogid:rglZ3AQuJfa1rFYIeZLqiYj+L4U559292 - false 32p radiolabel:?-?|tag:1-1|mutation:1-308 - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:P43321 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-529|ensemblfungi:YLR147C|uniprotkb:D6VYE2|uniprotkb:E9P8W3 psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:smd3_yeast(display_long)|uniprotkb:SMD3(gene name)|psi-mi:SMD3(display_short)|uniprotkb:YLR147C(locus name)|uniprotkb:L9634.6(orf name)|uniprotkb:snRNP core protein D3(gene name synonym) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616364|imex:IM-25038-3 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - refseq:NP_013248.1|sgd:S000004137|rcsb pdb:5YLZ|rcsb pdb:5ZWM|rcsb pdb:5ZWN|rcsb pdb:5ZWO|rcsb pdb:6BK8|rcsb pdb:6EXN|rcsb pdb:6G90|rcsb pdb:6J6G|rcsb pdb:6J6H|rcsb pdb:6J6N|rcsb pdb:6J6Q|rcsb pdb:6N7P|rcsb pdb:6N7R|rcsb pdb:6N7X|rcsb pdb:7B9V|ensemblfungi:YLR147C(gene)|ensemblfungi:YLR147C_mRNA(transcript)|go:"GO:0000243"(commitment complex)|go:"GO:0000387"(spliceosomal snRNP assembly)|go:"GO:0000398"(mRNA splicing, via spliceosome)|go:"GO:0003723"(RNA binding)|go:"GO:0003729"(mRNA binding)|go:"GO:0005682"(U5 snRNP)|go:"GO:0005685"(U1 snRNP)|go:"GO:0005686"(U2 snRNP)|go:"GO:0005687"(U4 snRNP)|go:"GO:0005689"(U12-type spliceosomal complex)|go:"GO:0034715"(pICln-Sm protein complex)|go:"GO:0034719"(SMN-Sm protein complex)|go:"GO:0046540"(U4/U6 x U5 tri-snRNP complex)|go:"GO:0071004"(U2-type prespliceosome)|go:"GO:0071011"(precatalytic spliceosome)|go:"GO:0071013"(catalytic step 2 spliceosome)|go:"GO:0097526"(spliceosomal tri-snRNP complex)|interpro:IPR001163(Like-Sm ribonucleoprotein, core)|interpro:IPR010920(Like-Sm ribonucleoprotein-related, core)|interpro:IPR027141|interpro:IPR034099|rcsb pdb:3JCM|rcsb pdb:5GAM|rcsb pdb:5GAN|rcsb pdb:5GAO|rcsb pdb:5GM6|rcsb pdb:5GMK|rcsb pdb:5LJ3|rcsb pdb:5LJ5|rcsb pdb:5LQW|rcsb pdb:5MPS|rcsb pdb:5MQ0|rcsb pdb:5NRL|rcsb pdb:5WSG|rcsb pdb:5Y88|dip:DIP-715N|mint:P43321 - - crc64:BB02A56C5AD70CF2 figure legend:Table 1, Suppl Fig4|comment:The spiceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate C complex 2mM ATP was added and a deletion mutant of actin premRNA was used|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2017/12/02 - rogid:ueJ4D76t8ilvz/p9qMzIGiCzqWk559292 - false 32p radiolabel:?-?|tag:1-1|mutation:1-308 - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:Q12309 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-484|uniprotkb:D6VYB5|ensemblfungi:YLR117C psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:clf1_yeast(display_long)|uniprotkb:CLF1(gene name)|psi-mi:CLF1(display_short)|uniprotkb:NTC77(gene name synonym)|uniprotkb:YLR117C(locus name)|uniprotkb:SYF3(gene name synonym)|uniprotkb:L2952(orf name)|uniprotkb:Crooked neck-like factor 1(gene name synonym)|uniprotkb:PRP19-associated complex protein 77(gene name synonym)|uniprotkb:Synthetic lethal with CDC40 protein 3(gene name synonym) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616364|imex:IM-25038-3 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - refseq:NP_013218.1|sgd:S000004107|rcsb pdb:6J6H|rcsb pdb:6J6N|rcsb pdb:6J6Q|rcsb pdb:5GM6|rcsb pdb:5GMK|rcsb pdb:5LJ3|rcsb pdb:5LJ5|rcsb pdb:5LQW|rcsb pdb:5MPS|rcsb pdb:5MQ0|rcsb pdb:5WSG|rcsb pdb:5Y88|rcsb pdb:5YLZ|rcsb pdb:6BK8|rcsb pdb:6EXN|rcsb pdb:6J6G|ensemblfungi:YLR117C(gene)|ensemblfungi:YLR117C_mRNA(transcript)|go:"GO:0000245"(spliceosomal complex assembly)|go:"GO:0000354"(cis assembly of pre-catalytic spliceosome)|go:"GO:0000398"(mRNA splicing, via spliceosome)|go:"GO:0000785"(chromatin)|go:"GO:0000974"(Prp19 complex)|go:"GO:0003682"(chromatin binding)|go:"GO:0003688"(DNA replication origin binding)|go:"GO:0006270"(DNA replication initiation)|go:"GO:0071004"(U2-type prespliceosome)|go:"GO:0071006"(U2-type catalytic step 1 spliceosome)|go:"GO:0071007"(U2-type catalytic step 2 spliceosome)|go:"GO:0071008"(U2-type post-mRNA release spliceosomal complex)|go:"GO:0071014"(post-mRNA release spliceosomal complex)|interpro:IPR003107(RNA-processing protein, HAT helix)|interpro:IPR011990(Tetratricopeptide-like helical)|dip:DIP-1685N|mint:Q12309 - - crc64:AE7D5EC5B3979E00 figure legend:Table 1, Suppl Fig4|comment:The spiceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate C complex 2mM ATP was added and a deletion mutant of actin premRNA was used|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2017/12/02 - rogid:VbNHO9YGUushIXHTgscFu4xCzC4559292 - false 32p radiolabel:?-?|tag:1-1|mutation:1-308 - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:Q07930 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-27110|uniprotkb:Q06901|uniprotkb:D6VY18|ensemblfungi:YLR016C psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:pml1_yeast(display_long)|uniprotkb:L1591(orf name)|uniprotkb:YLR016C(locus name)|uniprotkb:PML1(gene name)|psi-mi:PML1(display_short) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616364|imex:IM-25038-3 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - refseq:NP_013116.1|sgd:S000004006|dip:DIP-2149N|go:"GO:0005737"(cytoplasm)|go:"GO:0006406"(mRNA export from nucleus)|go:"GO:0051237"(maintenance of RNA location)|go:"GO:0070274"(RES complex)|interpro:IPR000253(Forkhead-associated)|interpro:IPR008984(SMAD/FHA domain)|rcsb pdb:2JKD|rcsb pdb:2MKC|rcsb pdb:2MY3|rcsb pdb:3ELS|rcsb pdb:3ELV|rcsb pdb:5GM6|rcsb pdb:5LQW|rcsb pdb:5ZWM|rcsb pdb:5ZWO|rcsb pdb:7DCO|ensemblfungi:YLR016C(gene)|ensemblfungi:YLR016C_mRNA(transcript)|go:"GO:0000398"(mRNA splicing, via spliceosome)|go:"GO:0003729"(mRNA binding)|go:"GO:0005634"(nucleus) - - crc64:DA39B4465EFFD378 figure legend:Table 1, Suppl Fig4|comment:The spiceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate C complex 2mM ATP was added and a deletion mutant of actin premRNA was used|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2017/12/02 - rogid:Q4q9xCj+AyRl430bDbu8yLp/Vak559292 - false 32p radiolabel:?-?|tag:1-1|mutation:1-308 - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:P32523 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-493|uniprotkb:Q07870|ensemblfungi:YLL036C|uniprotkb:D6VXW9 psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:prp19_yeast(display_long)|uniprotkb:PRP19(gene name)|psi-mi:PRP19(display_short)|uniprotkb:PSO4(gene name synonym)|uniprotkb:RING-type E3 ubiquitin transferase PRP19(gene name synonym)|uniprotkb:YLL036C(locus name) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616364|imex:IM-25038-3 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - refseq:NP_013064.1|sgd:S000003959|rcsb pdb:6EXN|rcsb pdb:6J6G|rcsb pdb:6J6H|rcsb pdb:6J6N|rcsb pdb:6J6Q|reactome:R-SCE-6781823|reactome:R-SCE-6782135|reactome:R-SCE-6782210|interpro:IPR015943(WD40/YVTN repeat-like)|interpro:IPR038959|rcsb pdb:1N87|rcsb pdb:2BAY|rcsb pdb:3LRV|rcsb pdb:4ZB4|rcsb pdb:5GM6|rcsb pdb:5GMK|rcsb pdb:5LJ5|rcsb pdb:5M8C|rcsb pdb:5MQ0|ensemblfungi:YLL036C(gene)|ensemblfungi:YLL036C_mRNA(transcript)|go:"GO:0000349"(generation of catalytic spliceosome for first transesterification step)|go:"GO:0000974"(Prp19 complex)|go:"GO:0004842"(ubiquitin-protein transferase activity)|go:"GO:0005634"(nucleus)|go:"GO:0005737"(cytoplasm)|go:"GO:0005739"(mitochondrion)|go:"GO:0005829"(cytosol)|go:"GO:0006281"(DNA repair)|go:"GO:0042802"(identical protein binding)|go:"GO:0061630"(ubiquitin protein ligase activity)|go:"GO:0070534"(protein K63-linked ubiquitination)|go:"GO:0071006"(U2-type catalytic step 1 spliceosome)|go:"GO:0071013"(catalytic step 2 spliceosome)|interpro:IPR003613(U box)|interpro:IPR011047(Quinonprotein alcohol dehydrogenase-like)|interpro:IPR013083(Zinc finger, RING/FYVE/PHD-type)|interpro:IPR013915(Pre-mRNA-splicing factor 19)|rcsb pdb:5WSG|rcsb pdb:5Y88|rcsb pdb:5YLZ|rcsb pdb:6BK8|dip:DIP-1112N|mint:P32523 - - crc64:A44D8C7DD6F64185 figure legend:Table 1, Suppl Fig4|comment:The spiceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate C complex 2mM ATP was added and a deletion mutant of actin premRNA was used|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2017/12/02 - rogid:/oQc2rJZ7WcCIx7cnqE6XSW/3Vk559292 - false 32p radiolabel:?-?|tag:1-1|mutation:1-308 - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:P21374 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-9382|ensemblfungi:YJR050W|uniprotkb:D6VWM1 psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:isy1_yeast(display_long)|uniprotkb:ISY1(gene name)|psi-mi:ISY1(display_short)|uniprotkb:UTR3(gene name synonym)|uniprotkb:YJR050W(locus name)|uniprotkb:J1657(orf name)|uniprotkb:NTC30(gene name synonym)|uniprotkb:Interactor of SYF1(gene name synonym)|uniprotkb:PRP19-associated complex protein 30(gene name synonym) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616364|imex:IM-25038-3 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - ensemblfungi:YJR050W(gene)|ensemblfungi:YJR050W_mRNA(transcript)|go:"GO:0000350"(generation of catalytic spliceosome for second transesterification step)|go:"GO:0000389"(mRNA 3'-splice site recognition)|go:"GO:0000974"(Prp19 complex)|go:"GO:0005737"(cytoplasm)|go:"GO:0071006"(U2-type catalytic step 1 spliceosome)|go:"GO:0071007"(U2-type catalytic step 2 spliceosome)|go:"GO:0071013"(catalytic step 2 spliceosome)|go:"GO:0071014"(post-mRNA release spliceosomal complex)|go:"GO:0071020"(post-spliceosomal complex)|interpro:IPR009360(Isy1-like splicing)|interpro:IPR029012|interpro:IPR037200|rcsb pdb:5GMK|rcsb pdb:5LJ3|rcsb pdb:5LJ5|rcsb pdb:6J6G|rcsb pdb:6J6H|rcsb pdb:6J6N|rcsb pdb:6J6Q|reactome:R-SCE-6781823|reactome:R-SCE-6782135|reactome:R-SCE-6782210|sgd:S000003811|refseq:NP_012584.3|mint:P21374|dip:DIP-1682N - - - figure legend:Table 1, Suppl Fig4|comment:The spiceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate C complex 2mM ATP was added and a deletion mutant of actin premRNA was used|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2017/12/02 - rogid:NHLAtd+wLxwtWQD8PsyzyQsfJOg559292 - false 32p radiolabel:?-?|tag:1-1|mutation:1-308 - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:P32524 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-603|intact:EBI-464832|uniprotkb:Q6Q5G7|uniprotkb:D6VVZ1|ensemblfungi:YJL203W psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:prp21_yeast(display_long)|uniprotkb:PRP21(gene name)|psi-mi:PRP21(display_short)|uniprotkb:SPP91(gene name synonym)|uniprotkb:YJL203W(locus name)|uniprotkb:J0322(orf name) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616364|imex:IM-25038-3 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - refseq:NP_012332.1|sgd:S000003739|dip:DIP-689N|mint:P32524|ensemblfungi:YJL203W(gene)|ensemblfungi:YJL203W_mRNA(transcript)|go:"GO:0000398"(mRNA splicing, via spliceosome)|go:"GO:0003723"(RNA binding)|go:"GO:0005686"(U2 snRNP)|go:"GO:0071004"(U2-type prespliceosome)|go:"GO:0071013"(catalytic step 2 spliceosome)|interpro:IPR000061(SWAP/Surp)|interpro:IPR022030|interpro:IPR035967|rcsb pdb:4DGW|rcsb pdb:5NRL|rcsb pdb:5ZWM|rcsb pdb:5ZWO|rcsb pdb:6G90|rcsb pdb:7DCO - - crc64:2E9DBA6F06759B3F figure legend:Table 1, Suppl Fig4|comment:The spiceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate C complex 2mM ATP was added and a deletion mutant of actin premRNA was used|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2017/12/02 - rogid:mOta2u2zOwtuZB+8s4z9DBL9iI0559292 - false 32p radiolabel:?-?|tag:1-1|mutation:1-308 - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:P53333 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-565|uniprotkb:D6VV55|ensemblfungi:YGR278W psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:cwc22_yeast(display_long)|uniprotkb:YGR278W(locus name)|uniprotkb:CWC22(gene name)|psi-mi:CWC22(display_short)|uniprotkb:Complexed with CEF1 protein 22(gene name synonym) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616364|imex:IM-25038-3 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - sgd:S000003510|refseq:NP_011794.3|ensemblfungi:YGR278W(gene)|ensemblfungi:YGR278W_mRNA(transcript)|go:"GO:0000398"(mRNA splicing, via spliceosome)|go:"GO:0003723"(RNA binding)|go:"GO:0005684"(U2-type spliceosomal complex)|go:"GO:0005737"(cytoplasm)|go:"GO:0071013"(catalytic step 2 spliceosome)|interpro:IPR003890(MIF4G-like, type 3)|interpro:IPR003891(Initiation factor eIF-4 gamma, MA3)|interpro:IPR016021(MIF4-like, type 1/2/3)|interpro:IPR016024(Armadillo-type fold)|rcsb pdb:5GM6|rcsb pdb:5GMK|rcsb pdb:5LQW|rcsb pdb:5MPS|rcsb pdb:5MQ0|rcsb pdb:5WSG|rcsb pdb:5YLZ|rcsb pdb:6BK8|rcsb pdb:6EXN|dip:DIP-1869N|mint:P53333|rcsb pdb:6J6H|rcsb pdb:6J6N|rcsb pdb:6J6Q|rcsb pdb:6J6G - - crc64:773E33C71E4A29D0 figure legend:Table 1, Suppl Fig4|comment:The spiceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate C complex 2mM ATP was added and a deletion mutant of actin premRNA was used|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2017/12/02 - rogid:V/pQcpK6QPwE9cfqwmQZ0uqGpZk559292 - false 32p radiolabel:?-?|tag:1-1|mutation:1-308 - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:P53277 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-23308|uniprotkb:D6VUR1|ensemblfungi:YGR129W psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:syf2_yeast(display_long)|uniprotkb:SYF2(gene name)|psi-mi:SYF2(display_short)|uniprotkb:NTC31(gene name synonym)|uniprotkb:Synthetic lethal with CDC40 protein 2(gene name synonym)|uniprotkb:PRP19 complex protein 31(gene name synonym)|uniprotkb:YGR129W(locus name) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616364|imex:IM-25038-3 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - sgd:S000003361|refseq:NP_011645.3|dip:DIP-1684N|mint:P53277|interpro:IPR013260(mRNA splicing factor SYF2)|ensemblfungi:YGR129W(gene)|rcsb pdb:5GM6|rcsb pdb:5GMK|rcsb pdb:5MPS|rcsb pdb:5MQ0|rcsb pdb:5WSG|rcsb pdb:5Y88|rcsb pdb:5YLZ|rcsb pdb:6BK8|rcsb pdb:6EXN|rcsb pdb:6J6G|rcsb pdb:6J6H|rcsb pdb:6J6N|rcsb pdb:6J6Q|ensemblfungi:YGR129W_mRNA(transcript)|go:"GO:0000398"(mRNA splicing, via spliceosome)|go:"GO:0000974"(Prp19 complex)|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0071004"(U2-type prespliceosome)|go:"GO:0071006"(U2-type catalytic step 1 spliceosome)|go:"GO:0071007"(U2-type catalytic step 2 spliceosome)|go:"GO:0071008"(U2-type post-mRNA release spliceosomal complex) - - - figure legend:Table 1, Suppl Fig4|comment:The spiceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate C complex 2mM ATP was added and a deletion mutant of actin premRNA was used|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2017/12/02 - rogid:uVAPXi98XAE2k7IPXvd4d+KNknw559292 - false 32p radiolabel:?-?|tag:1-1|mutation:1-308 - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:Q00723 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-841|uniprotkb:D6VUK7|ensemblfungi:YGR075C psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:prp38_yeast(display_long)|uniprotkb:YGR075C(locus name)|uniprotkb:PRP38(gene name)|psi-mi:PRP38(display_short)|uniprotkb:Pre-mRNA-processing factor 38(gene name synonym) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616364|imex:IM-25038-3 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - sgd:S000003307|refseq:NP_011589.3|dip:DIP-1648N|mint:Q00723|ensemblfungi:YGR075C(gene)|ensemblfungi:YGR075C_mRNA(transcript)|go:"GO:0000388"("spliceosome conformational change to release U4 (or U4atac) and U1 (or U11)")|go:"GO:0046540"(U4/U6 x U5 tri-snRNP complex)|go:"GO:0071011"(precatalytic spliceosome)|interpro:IPR005037(PRP38)|rcsb pdb:5NRL|rcsb pdb:5ZWO - - crc64:62EB225947AF4349 figure legend:Table 1, Suppl Fig4|comment:The spiceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate C complex 2mM ATP was added and a deletion mutant of actin premRNA was used|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2017/12/02 - rogid:2C+lYBvjAO0jl1CAy0k1Q+Qv7ew559292 - false 32p radiolabel:?-?|tag:1-1|mutation:1-308 - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:Q02260 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-585|uniprotkb:D6VUK6|ensemblfungi:YGR074W psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:smd1_yeast(display_long)|uniprotkb:YGR074W(locus name)|uniprotkb:SMD1(gene name)|psi-mi:SMD1(display_short)|uniprotkb:snRNP core protein D1(gene name synonym) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616364|imex:IM-25038-3 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - sgd:S000003306|refseq:NP_011588.3|dip:DIP-227N|mint:Q02260|ensemblfungi:YGR074W(gene)|ensemblfungi:YGR074W_mRNA(transcript)|go:"GO:0000243"(commitment complex)|go:"GO:0000387"(spliceosomal snRNP assembly)|go:"GO:0000398"(mRNA splicing, via spliceosome)|go:"GO:0003723"(RNA binding)|go:"GO:0003729"(mRNA binding)|go:"GO:0005634"(nucleus)|go:"GO:0005682"(U5 snRNP)|go:"GO:0005685"(U1 snRNP)|go:"GO:0005686"(U2 snRNP)|go:"GO:0005689"(U12-type spliceosomal complex)|go:"GO:0005829"(cytosol)|go:"GO:0030532"(small nuclear ribonucleoprotein complex)|go:"GO:0034715"(pICln-Sm protein complex)|go:"GO:0005687"(U4 snRNP)|go:"GO:0034719"(SMN-Sm protein complex)|go:"GO:0046540"(U4/U6 x U5 tri-snRNP complex)|go:"GO:0071004"(U2-type prespliceosome)|go:"GO:0071011"(precatalytic spliceosome)|go:"GO:0071013"(catalytic step 2 spliceosome)|go:"GO:0097526"(spliceosomal tri-snRNP complex)|interpro:IPR001163(Like-Sm ribonucleoprotein, core)|interpro:IPR010920(Like-Sm ribonucleoprotein-related, core)|interpro:IPR027141|rcsb pdb:3JCM|rcsb pdb:5GAM|rcsb pdb:5GAN|rcsb pdb:5GAO|rcsb pdb:5GM6|rcsb pdb:5GMK|rcsb pdb:5LJ3|rcsb pdb:5LJ5|rcsb pdb:5LQW|rcsb pdb:5MPS|rcsb pdb:5MQ0|rcsb pdb:5NRL|rcsb pdb:5WSG|rcsb pdb:5Y88|rcsb pdb:5YLZ|rcsb pdb:5ZWM|rcsb pdb:5ZWN|rcsb pdb:5ZWO|rcsb pdb:6BK8|rcsb pdb:6EXN|rcsb pdb:6G90|rcsb pdb:6J6G|rcsb pdb:6J6H|rcsb pdb:6J6N|rcsb pdb:6J6Q|rcsb pdb:6N7P|rcsb pdb:6N7R|rcsb pdb:6N7X|rcsb pdb:7B9V - - crc64:9929F21EB9218447 figure legend:Table 1, Suppl Fig4|comment:The spiceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate C complex 2mM ATP was added and a deletion mutant of actin premRNA was used|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2017/12/02 - rogid:SvOKDbY05xgJXKZ8uS+keXsC41I559292 - false 32p radiolabel:?-?|tag:1-1|mutation:1-308 - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:P33411 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-13844|uniprotkb:Q02459|uniprotkb:Q05724|ensemblfungi:YGR006W|uniprotkb:D6VUE3 psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:prp18_yeast(display_long)|uniprotkb:PRP18(gene name)|psi-mi:PRP18(display_short)|uniprotkb:YGR006W(locus name) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616364|imex:IM-25038-3 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - ensemblfungi:YGR006W(gene)|ensemblfungi:YGR006W_mRNA(transcript)|go:"GO:0000350"(generation of catalytic spliceosome for second transesterification step)|go:"GO:0000398"(mRNA splicing, via spliceosome)|go:"GO:0005682"(U5 snRNP)|go:"GO:0046540"(U4/U6 x U5 tri-snRNP complex)|go:"GO:0071021"(U2-type post-spliceosomal complex)|go:"GO:0071048"|interpro:IPR004098(Prp18)|interpro:IPR039979|rcsb pdb:1DVK|rcsb pdb:5MPS|rcsb pdb:5MQ0|rcsb pdb:5WSG|rcsb pdb:5YLZ|rcsb pdb:6BK8|rcsb pdb:6EXN|refseq:NP_011520.2|sgd:S000003238|mint:P33411|dip:DIP-1110N - - - figure legend:Table 1, Suppl Fig4|comment:The spiceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate C complex 2mM ATP was added and a deletion mutant of actin premRNA was used|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2017/12/02 - rogid:3OaVOaHslViUjLZSTUDVIVMo4WY559292 - false 32p radiolabel:?-?|tag:1-1|mutation:1-308 - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:P46947 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-24073|uniprotkb:D6VTX8|ensemblfungi:YGL174W psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:cwc26_yeast(display_long)|uniprotkb:YGL174W(locus name)|uniprotkb:G1642(orf name)|uniprotkb:BUD13(gene name)|psi-mi:BUD13(display_short)|uniprotkb:SLC7(gene name synonym)|uniprotkb:Complexed with CEF1 protein 26(gene name synonym)|uniprotkb:Bud site selection protein 13(gene name synonym)|uniprotkb:Synthetic lethal with CLF1 protein 7(gene name synonym)|uniprotkb:CWC26(gene name synonym) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616364|imex:IM-25038-3 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - sgd:S000003142|refseq:NP_011341.3|dip:DIP-2075N|mint:P46947|ensemblfungi:YGL174W(gene)|ensemblfungi:YGL174W_mRNA(transcript)|go:"GO:0000398"(mRNA splicing, via spliceosome)|go:"GO:0005634"(nucleus)|go:"GO:0005684"(U2-type spliceosomal complex)|go:"GO:0005737"(cytoplasm)|go:"GO:0070274"(RES complex)|interpro:IPR018609|rcsb pdb:2MKC|rcsb pdb:2MY2|rcsb pdb:4UQT|rcsb pdb:5GM6|rcsb pdb:5LQW|rcsb pdb:5ZWM|rcsb pdb:5ZWO - - crc64:1584B390121325CD figure legend:Table 1, Suppl Fig4|comment:The spiceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate C complex 2mM ATP was added and a deletion mutant of actin premRNA was used|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2017/12/02 - rogid:JGmiLd4cWl/n2wZVMju48AdYsNk559292 - false 32p radiolabel:?-?|tag:1-1|mutation:1-308 - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:P52868 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-560|uniprotkb:Q6B1S2|uniprotkb:D6VU20|ensemblfungi:YGL128C psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:cwc23_yeast(display_long)|uniprotkb:YGL128C(locus name)|uniprotkb:G2861(orf name)|uniprotkb:CWC23(gene name)|psi-mi:CWC23(display_short)|uniprotkb:Complexed with CEF1 protein 23(gene name synonym) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616364|imex:IM-25038-3 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - refseq:NP_011387.2|sgd:S000003096|ensemblfungi:YGL128C(gene)|ensemblfungi:YGL128C_mRNA(transcript)|go:"GO:0000390"(spliceosomal complex disassembly)|go:"GO:0005684"(U2-type spliceosomal complex)|go:"GO:0005737"(cytoplasm)|interpro:IPR001623(Heat shock protein DnaJ, N-terminal)|interpro:IPR018253|interpro:IPR036869|rcsb pdb:5Y88|dip:DIP-6831N|mint:P52868 - - crc64:AA1D0894328CAEA5 figure legend:Table 1, Suppl Fig4|comment:The spiceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate C complex 2mM ATP was added and a deletion mutant of actin premRNA was used|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2017/12/02 - rogid:eRgXchjfvYBUTrkj+wWdEiWX3o8559292 - false 32p radiolabel:?-?|tag:1-1|mutation:1-308 - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:P53131 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-505|ensemblfungi:YGL120C|uniprotkb:D6VU28 psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:prp43_yeast(display_long)|uniprotkb:PRP43(gene name)|psi-mi:PRP43(display_short)|uniprotkb:Helicase JA1(gene name synonym)|uniprotkb:YGL120C(locus name) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616364|imex:IM-25038-3 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - go:"GO:0071014"(post-mRNA release spliceosomal complex)|go:"GO:0140603"(obsolete ATP hydrolysis activity)|interpro:IPR001650(DNA/RNA helicase, C-terminal)|interpro:IPR002464(DNA/RNA helicase, ATP-dependent, DEAH-box type, conserved site)|interpro:IPR007502(Helicase-associated region)|interpro:IPR011545(DNA/RNA helicase, DEAD/DEAH box type, N-terminal)|interpro:IPR011709(Region of unknown function DUF1605)|interpro:IPR014001(DEAD-like helicase, N-terminal)|interpro:IPR027417|interpro:IPR044756|rcsb pdb:2XAU|rcsb pdb:3KX2|rcsb pdb:5I8Q|rcsb pdb:5JPT|rcsb pdb:5Y88|refseq:NP_011395.1|sgd:S000003088|ensemblfungi:YGL120C(gene)|ensemblfungi:YGL120C_mRNA(transcript)|go:"GO:0000390"(spliceosomal complex disassembly)|go:"GO:0000462"("maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)")|go:"GO:0000463"("maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)")|go:"GO:0000466"("maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)")|go:"GO:0003723"(RNA binding)|go:"GO:0003724"(RNA helicase activity)|go:"GO:0003729"(mRNA binding)|go:"GO:0005524"(ATP binding)|go:"GO:0005681"(spliceosomal complex)|go:"GO:0005739"(mitochondrion)|go:"GO:0006364"(rRNA processing)|go:"GO:0030490"(maturation of SSU-rRNA)|go:"GO:0030686"(90S preribosome)|go:"GO:0042273"(ribosomal large subunit biogenesis)|dip:DIP-5152N|mint:P53131 - - crc64:DA8A085EC50FF430 figure legend:Table 1, Suppl Fig4|comment:The spiceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate C complex 2mM ATP was added and a deletion mutant of actin premRNA was used|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2017/12/02 - rogid:45MX/uTINAjulwkB0vGKeimS+8c559292 - false 32p radiolabel:?-?|tag:1-1|mutation:1-308 - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:P40204 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-637|uniprotkb:D6VTL2|ensemblfungi:YFL017W-A psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:ruxg_yeast(display_long)|uniprotkb:SNP2(gene name synonym)|uniprotkb:YFL017Bw(orf name)|uniprotkb:YFL017W-A(locus name)|uniprotkb:SMX2(gene name)|psi-mi:SMX2(display_short)|uniprotkb:Sm protein G(gene name synonym) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616364|imex:IM-25038-3 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - refseq:NP_116636.1|sgd:S000002965|dip:DIP-6683N|mint:P40204|ensemblfungi:YFL017W-A(gene)|ensemblfungi:YFL017W-A_mRNA(transcript)|go:"GO:0000387"(spliceosomal snRNP assembly)|go:"GO:0000398"(mRNA splicing, via spliceosome)|go:"GO:0003723"(RNA binding)|go:"GO:0005682"(U5 snRNP)|go:"GO:0005685"(U1 snRNP)|go:"GO:0005686"(U2 snRNP)|go:"GO:0005687"(U4 snRNP)|go:"GO:0005689"(U12-type spliceosomal complex)|go:"GO:0034719"(SMN-Sm protein complex)|go:"GO:0046540"(U4/U6 x U5 tri-snRNP complex)|go:"GO:0071004"(U2-type prespliceosome)|go:"GO:0071011"(precatalytic spliceosome)|go:"GO:0071013"(catalytic step 2 spliceosome)|go:"GO:0097526"(spliceosomal tri-snRNP complex)|go:"GO:1990904"(ribonucleoprotein complex)|interpro:IPR001163(Like-Sm ribonucleoprotein, core)|interpro:IPR010920(Like-Sm ribonucleoprotein-related, core)|interpro:IPR034098|interpro:IPR044641|rcsb pdb:3JCM|rcsb pdb:5GAM|rcsb pdb:5GAN|rcsb pdb:5GAO|rcsb pdb:5GM6|rcsb pdb:5GMK|rcsb pdb:5LJ3|rcsb pdb:5LJ5|rcsb pdb:5LQW|rcsb pdb:5MPS|rcsb pdb:5MQ0|rcsb pdb:5NRL|rcsb pdb:5WSG|rcsb pdb:5Y88|rcsb pdb:5YLZ|rcsb pdb:5ZWM|rcsb pdb:5ZWN|rcsb pdb:5ZWO|rcsb pdb:6BK8|rcsb pdb:6EXN|rcsb pdb:6G90|rcsb pdb:6J6G|rcsb pdb:6J6H|rcsb pdb:6J6N|rcsb pdb:6J6Q|rcsb pdb:6N7P|rcsb pdb:6N7R|rcsb pdb:6N7X|rcsb pdb:7B9V|rcsb pdb:7DCO - - crc64:5C6208B8C5A9AB33 figure legend:Table 1, Suppl Fig4|comment:The spiceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate C complex 2mM ATP was added and a deletion mutant of actin premRNA was used|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2017/12/02 - rogid:Wh37rGoSJFpJ5o+CuNkBnpiu/5s559292 - false 32p radiolabel:?-?|tag:1-1|mutation:1-308 - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:Q03375 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-30409|uniprotkb:D6VTA5|ensemblfungi:YDR482C psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:cwc21_yeast(display_long)|uniprotkb:YDR482C(locus name)|uniprotkb:Complexed with CEF1 protein 21(gene name synonym)|uniprotkb:CWC21(gene name)|psi-mi:CWC21(display_short) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616364|imex:IM-25038-3 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - sgd:S000002890|refseq:NP_010770.3|dip:DIP-1847N|mint:Q03375|ensemblfungi:YDR482C(gene)|ensemblfungi:YDR482C_mRNA(transcript)|go:"GO:0000398"(mRNA splicing, via spliceosome)|go:"GO:0005684"(U2-type spliceosomal complex)|go:"GO:0005737"(cytoplasm)|interpro:IPR013170(mRNA splicing factor, Cwf21)|rcsb pdb:5GM6|rcsb pdb:5GMK|rcsb pdb:5LJ3|rcsb pdb:5LJ5|rcsb pdb:5MPS|rcsb pdb:5MQ0|rcsb pdb:5WSG|rcsb pdb:5YLZ|rcsb pdb:6BK8|rcsb pdb:6EXN|rcsb pdb:6J6G|rcsb pdb:6J6H|rcsb pdb:6J6N|rcsb pdb:6J6Q - - crc64:9723E1C0A393508B figure legend:Table 1, Suppl Fig4|comment:The spiceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate C complex 2mM ATP was added and a deletion mutant of actin premRNA was used|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2017/12/02 - rogid:dXmh6RsUTTtGZ8d1CnRgs1Ma+VI559292 - false 32p radiolabel:?-?|tag:1-1|mutation:1-308 - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:Q04048 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-540|uniprotkb:D6VT47|ensemblfungi:YDR416W psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:syf1_yeast(display_long)|uniprotkb:SYF1(gene name)|psi-mi:SYF1(display_short)|uniprotkb:NTC90(gene name synonym)|uniprotkb:Synthetic lethal with CDC40 protein 1(gene name synonym)|uniprotkb:PRP19-associated complex protein 90(gene name synonym)|uniprotkb:YDR416W(locus name) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616364|imex:IM-25038-3 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - refseq:NP_010704.1|sgd:S000002824|rcsb pdb:5YLZ|rcsb pdb:6BK8|rcsb pdb:6EXN|rcsb pdb:6J6G|rcsb pdb:6J6H|rcsb pdb:6J6N|rcsb pdb:6J6Q|reactome:R-SCE-6781823|reactome:R-SCE-6782135|reactome:R-SCE-6782210|rcsb pdb:5Y88|ensemblfungi:YDR416W(gene)|ensemblfungi:YDR416W_mRNA(transcript)|go:"GO:0000349"(generation of catalytic spliceosome for first transesterification step)|go:"GO:0000398"(mRNA splicing, via spliceosome)|go:"GO:0000974"(Prp19 complex)|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0071004"(U2-type prespliceosome)|go:"GO:0071006"(U2-type catalytic step 1 spliceosome)|go:"GO:0071007"(U2-type catalytic step 2 spliceosome)|go:"GO:0071008"(U2-type post-mRNA release spliceosomal complex)|go:"GO:0071014"(post-mRNA release spliceosomal complex)|interpro:IPR003107(RNA-processing protein, HAT helix)|interpro:IPR011990(Tetratricopeptide-like helical)|rcsb pdb:5GM6|rcsb pdb:5GMK|rcsb pdb:5LJ3|rcsb pdb:5LJ5|rcsb pdb:5LQW|rcsb pdb:5MPS|rcsb pdb:5MQ0|rcsb pdb:5WSG|dip:DIP-1681N|mint:Q04048 - - crc64:ED4368A624A40E7C figure legend:Table 1, Suppl Fig4|comment:The spiceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate C complex 2mM ATP was added and a deletion mutant of actin premRNA was used|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2017/12/02 - rogid:+1TwiqsQv1IcWxuoIWcJGtTCUFU559292 - false 32p radiolabel:?-?|tag:1-1|mutation:1-308 - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:P40968 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-13834|uniprotkb:Q06341|ensemblfungi:YDR364C|uniprotkb:D6VSZ3 psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:prp17_yeast(display_long)|uniprotkb:CDC40(gene name)|psi-mi:CDC40(display_short)|uniprotkb:PRP17(gene name synonym)|uniprotkb:SLU4(gene name synonym)|uniprotkb:XRS2(gene name synonym)|uniprotkb:YDR364C(locus name)|uniprotkb:D9481.11(orf name)|uniprotkb:Cell division control protein 40(gene name synonym) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616364|imex:IM-25038-3 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - ensemblfungi:YDR364C(gene)|ensemblfungi:YDR364C_mRNA(transcript)|go:"GO:0000348"(mRNA branch site recognition)|go:"GO:0000350"(generation of catalytic spliceosome for second transesterification step)|go:"GO:0000389"(mRNA 3'-splice site recognition)|go:"GO:0005681"(spliceosomal complex)|go:"GO:0034399"(nuclear periphery)|go:"GO:0071013"(catalytic step 2 spliceosome)|interpro:IPR001680(WD40 repeat)|interpro:IPR015943(WD40/YVTN repeat-like)|interpro:IPR020472|interpro:IPR032847|interpro:IPR036322|rcsb pdb:5GM6|rcsb pdb:5GMK|rcsb pdb:5MPS|rcsb pdb:5MQ0|rcsb pdb:5WSG|rcsb pdb:5Y88|rcsb pdb:5YLZ|rcsb pdb:6BK8|rcsb pdb:6EXN|rcsb pdb:6J6G|rcsb pdb:6J6H|rcsb pdb:6J6N|rcsb pdb:6J6Q|sgd:S000002772|refseq:NP_010652.3|dip:DIP-1111N - - crc64:40AED5DB35319147 figure legend:Table 1, Suppl Fig4|comment:The spiceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate C complex 2mM ATP was added and a deletion mutant of actin premRNA was used|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2017/12/02 - rogid:KoJjWDas71e2U/bQRs+yQFpbTk4559292 - false 32p radiolabel:?-?|tag:1-1|mutation:1-308 - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:Q03772 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-36780|uniprotkb:D6VSE3|ensemblfungi:YDR163W psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:cwc15_yeast(display_long)|uniprotkb:CWC15(gene name)|psi-mi:CWC15(display_short)|uniprotkb:Complexed with CEF1 protein 15(gene name synonym)|uniprotkb:YDR163W(locus name)|uniprotkb:YD8358.17(orf name) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616364|imex:IM-25038-3 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - sgd:S000002570|interpro:IPR006973(Cwf15/Cwc15 cell cycle control protein)|refseq:NP_010447.3|rcsb pdb:6J6G|rcsb pdb:6J6H|rcsb pdb:6J6N|rcsb pdb:6J6Q|rcsb pdb:5MPS|rcsb pdb:5MQ0|rcsb pdb:5WSG|rcsb pdb:5GM6|rcsb pdb:5GMK|rcsb pdb:5LJ3|rcsb pdb:6BK8|rcsb pdb:6EXN|rcsb pdb:5Y88|rcsb pdb:5YLZ|ensemblfungi:YDR163W(gene)|ensemblfungi:YDR163W_mRNA(transcript)|go:"GO:0000398"(mRNA splicing, via spliceosome)|go:"GO:0003723"(RNA binding)|go:"GO:0005634"(nucleus)|go:"GO:0005684"(U2-type spliceosomal complex)|go:"GO:0045292"(mRNA cis splicing, via spliceosome)|go:"GO:0071013"(catalytic step 2 spliceosome)|rcsb pdb:7DCO - - - figure legend:Table 1, Suppl Fig4|comment:The spiceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate C complex 2mM ATP was added and a deletion mutant of actin premRNA was used|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2017/12/02 - rogid:raWAr5hiD2qEEeUG/lcMNpo1lhM559292 - false 32p radiolabel:?-?|tag:1-1|mutation:1-308 - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:Q02775 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-17379|uniprotkb:P89902|uniprotkb:Q6Q5U3|uniprotkb:D6VS75|ensemblfungi:YDR088C psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:slu7_yeast(display_long)|uniprotkb:SLU7(gene name)|psi-mi:SLU7(display_short)|uniprotkb:YDR088C(locus name)|uniprotkb:D4483(orf name)|uniprotkb:SLT17(gene name synonym)|uniprotkb:Synthetic lethal with U5 snRNA protein 7(gene name synonym)|uniprotkb:Synthetic lethal with U2 snRNA protein 17(gene name synonym) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616364|imex:IM-25038-3 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - refseq:NP_010373.3|sgd:S000002495|ensemblfungi:YDR088C(gene)|ensemblfungi:YDR088C_mRNA(transcript)|go:"GO:0000350"(generation of catalytic spliceosome for second transesterification step)|go:"GO:0000386"(second spliceosomal transesterification activity)|go:"GO:0005681"(spliceosomal complex)|go:"GO:0008270"(zinc ion binding)|go:"GO:0008380"(RNA splicing)|go:"GO:0030628"(pre-mRNA 3'-splice site binding)|go:"GO:0071021"(U2-type post-spliceosomal complex)|interpro:IPR001878(Zinc finger, CCHC-type)|interpro:IPR021715|interpro:IPR036875|interpro:IPR039974|rcsb pdb:5MPS|rcsb pdb:5MQ0|rcsb pdb:6BK8|rcsb pdb:6EXN|rcsb pdb:5YLZ|mint:Q02775|dip:DIP-220N - - crc64:6405458FC367A7D3 figure legend:Table 1, Suppl Fig4|comment:The spiceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate C complex 2mM ATP was added and a deletion mutant of actin premRNA was used|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2017/12/02 - rogid:an3IJpPrRlLX0cALtTMg4C2JxUI559292 - false 32p radiolabel:?-?|tag:1-1|mutation:1-308 - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:Q12046 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-553|ensemblfungi:YDL209C|uniprotkb:D6VRE5 psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:cwc2_yeast(display_long)|uniprotkb:CWC2(gene name)|psi-mi:CWC2(display_short)|uniprotkb:YDL209C(locus name)|uniprotkb:D1041(orf name)|uniprotkb:NTC40(gene name synonym)|uniprotkb:SLC3(gene name synonym)|uniprotkb:Complexed with CEF1 protein 2(gene name synonym)|uniprotkb:PRP19-associated complex protein 40(gene name synonym)|uniprotkb:Synthetic lethal with CLF1 protein 3(gene name synonym) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616364|imex:IM-25038-3 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - interpro:IPR000571(Zinc finger, CCCH-type)|interpro:IPR012677(Nucleotide-binding, alpha-beta plait)|interpro:IPR032297|interpro:IPR034181|interpro:IPR035979|interpro:IPR039171|interpro:IPR039173|rcsb pdb:3TP2|rcsb pdb:3U1L|rcsb pdb:3U1M|rcsb pdb:5GM6|rcsb pdb:5GMK|rcsb pdb:5LJ3|rcsb pdb:5LJ5|rcsb pdb:5LQW|rcsb pdb:5MPS|rcsb pdb:5MQ0|rcsb pdb:5WSG|rcsb pdb:5Y88|rcsb pdb:5YLZ|rcsb pdb:6BK8|rcsb pdb:6EXN|rcsb pdb:6J6G|rcsb pdb:6J6H|rcsb pdb:6J6N|refseq:NP_010072.1|sgd:S000002368|ensemblfungi:YDL209C(gene)|ensemblfungi:YDL209C_mRNA(transcript)|go:"GO:0000387"(spliceosomal snRNP assembly)|go:"GO:0000398"(mRNA splicing, via spliceosome)|go:"GO:0000974"(Prp19 complex)|go:"GO:0007049"(cell cycle)|go:"GO:0017070"(U6 snRNA binding)|go:"GO:0033120"(positive regulation of RNA splicing)|go:"GO:0036002"(pre-mRNA binding)|go:"GO:0045292"(mRNA cis splicing, via spliceosome)|go:"GO:0045787"(positive regulation of cell cycle)|go:"GO:0046872"(metal ion binding)|go:"GO:0071006"(U2-type catalytic step 1 spliceosome)|go:"GO:0071007"(U2-type catalytic step 2 spliceosome)|interpro:IPR000504(RNA recognition motif, RNP-1)|rcsb pdb:6J6Q|dip:DIP-5164N - - crc64:371734A11DFDB339 figure legend:Table 1, Suppl Fig4|comment:The spiceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate C complex 2mM ATP was added and a deletion mutant of actin premRNA was used|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2017/12/02 - rogid:bCvRFfQxZ4QaBZqqOyQP7ye/UeQ559292 - false 32p radiolabel:?-?|tag:1-1|mutation:1-308 - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:Q07350 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-688|uniprotkb:Q05434|ensemblfungi:YDL043C|uniprotkb:D6VRV3 psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:prp11_yeast(display_long)|uniprotkb:PRP11(gene name)|psi-mi:PRP11(display_short)|uniprotkb:PRP10(gene name synonym)|uniprotkb:RNA11(gene name synonym)|uniprotkb:YDL043C(locus name)|uniprotkb:D2711(orf name) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616364|imex:IM-25038-3 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - refseq:NP_010241.1|sgd:S000002201|ensemblfungi:YDL043C(gene)|ensemblfungi:YDL043C_mRNA(transcript)|go:"GO:0000245"(spliceosomal complex assembly)|go:"GO:0003723"(RNA binding)|go:"GO:0005686"(U2 snRNP)|go:"GO:0008270"(zinc ion binding)|go:"GO:0071004"(U2-type prespliceosome)|go:"GO:0071013"(catalytic step 2 spliceosome)|interpro:IPR003604(Zinc finger, U1-type)|interpro:IPR031781|interpro:IPR036236|rcsb pdb:4DGW|rcsb pdb:5GM6|rcsb pdb:5NRL|rcsb pdb:5ZWM|rcsb pdb:5ZWO|rcsb pdb:6G90|dip:DIP-1001N|mint:Q07350 - - crc64:D8BA3BB27F36F06F figure legend:Table 1, Suppl Fig4|comment:The spiceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate C complex 2mM ATP was added and a deletion mutant of actin premRNA was used|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2017/12/02 - rogid:xyunpB0FKbtjXifS4hyBHPmL4zs559292 - false 32p radiolabel:?-?|tag:1-1|mutation:1-308 - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:P19736 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-511|ensemblfungi:YDL030W|uniprotkb:D6VRW2 psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:prp9_yeast(display_long)|uniprotkb:PRP9(gene name)|psi-mi:PRP9(display_short)|uniprotkb:D2773(orf name)|uniprotkb:YDL030W(locus name) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616364|imex:IM-25038-3 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - ensemblfungi:YDL030W(gene)|ensemblfungi:YDL030W_mRNA(transcript)|go:"GO:0000395"(mRNA 5'-splice site recognition)|go:"GO:0000398"(mRNA splicing, via spliceosome)|go:"GO:0003723"(RNA binding)|go:"GO:0005681"(spliceosomal complex)|go:"GO:0008270"(zinc ion binding)|go:"GO:0071004"(U2-type prespliceosome)|interpro:IPR000690(Zinc finger, C2H2-type matrin)|interpro:IPR003604(Zinc finger, U1-type)|interpro:IPR013087(Zinc finger, C2H2-type/integrase, DNA-binding)|interpro:IPR022755|interpro:IPR024598|interpro:IPR031590|interpro:IPR031774|interpro:IPR031776|interpro:IPR036236|refseq:NP_010254.1|sgd:S000002188|rcsb pdb:4DGW|rcsb pdb:5NRL|rcsb pdb:5ZWM|rcsb pdb:5ZWO|rcsb pdb:6G90|dip:DIP-651N|mint:P19736 - - crc64:9020885E620488F3 figure legend:Table 1, Suppl Fig4|comment:The spiceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate C complex 2mM ATP was added and a deletion mutant of actin premRNA was used|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2017/12/02 - rogid:gdiY5/Azr3xdR4IiaMsyAcxJyXA559292 - false 32p radiolabel:?-?|tag:1-1|mutation:1-308 - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:P36048 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-243|uniprotkb:D6VX27|ensemblfungi:YKL173W psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:sn114_yeast(display_long)|uniprotkb:GIN10(gene name synonym)|uniprotkb:YKL173W(locus name)|uniprotkb:YKL637(orf name)|uniprotkb:SNU114(gene name)|psi-mi:SNU114(display_short)|uniprotkb:Growth inhibitory protein 10(gene name synonym)|uniprotkb:114 kDa U5 small nuclear ribonucleoprotein component(gene name synonym) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616364|imex:IM-25038-3 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - refseq:NP_012748.1|sgd:S000001656|mint:P36048|dip:DIP-759N|ensemblfungi:YKL173W(gene)|ensemblfungi:YKL173W_mRNA(transcript)|go:"GO:0000244"(spliceosomal tri-snRNP complex assembly)|go:"GO:0000349"(generation of catalytic spliceosome for first transesterification step)|go:"GO:0000388"("spliceosome conformational change to release U4 (or U4atac) and U1 (or U11)")|go:"GO:0000398"(mRNA splicing, via spliceosome)|go:"GO:0003924"(GTPase activity)|go:"GO:0005525"(GTP binding)|go:"GO:0005634"(nucleus)|go:"GO:0005682"(U5 snRNP)|go:"GO:0030623"(U5 snRNA binding)|go:"GO:0046540"(U4/U6 x U5 tri-snRNP complex)|go:"GO:0071007"(U2-type catalytic step 2 spliceosome)|go:"GO:1990904"(ribonucleoprotein complex)|interpro:IPR000640(Translation elongation factor EFG/EF2, C-terminal)|interpro:IPR000795(Protein synthesis factor, GTP-binding)|interpro:IPR004161(Translation elongation factor EFTu/EF1A, domain 2)|interpro:IPR005517(Translation elongation factor EFG/EF2, domain IV)|interpro:IPR009000(Translation elongation and initiation factors/Ribosomal, beta-barrel)|interpro:IPR014721(Ribosomal protein S5 domain 2-type fold)|interpro:IPR020568|interpro:IPR027417|interpro:IPR031950|interpro:IPR035647|interpro:IPR035655|interpro:IPR044121|rcsb pdb:3JCM|rcsb pdb:5GAM|rcsb pdb:5GAN|rcsb pdb:5GM6|rcsb pdb:5GMK|rcsb pdb:5LJ3|rcsb pdb:5LJ5|rcsb pdb:5LQW|rcsb pdb:5MPS|rcsb pdb:5MQ0|rcsb pdb:5NRL|rcsb pdb:5WSG|rcsb pdb:5Y88|rcsb pdb:5YLZ|rcsb pdb:5ZWM|rcsb pdb:5ZWO|rcsb pdb:6BK8|rcsb pdb:6EXN|rcsb pdb:6J6G|rcsb pdb:6J6H|rcsb pdb:6J6N|rcsb pdb:6J6Q|rcsb pdb:6TEO - - crc64:5CE30CEFF154D013 figure legend:Table 1, Suppl Fig4|comment:The spiceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate C complex 2mM ATP was added and a deletion mutant of actin premRNA was used|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2017/12/02 - rogid:eHDUPadk87+Qb525xpL1tMWb3S4559292 - false 32p radiolabel:?-?|tag:1-1|mutation:1-308 - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:P28320 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-26795|uniprotkb:D6VXJ3|ensemblfungi:YKL095W psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:yju2_yeast(display_long)|uniprotkb:YKL095W(locus name)|uniprotkb:YKL442(orf name)|uniprotkb:YJU2(gene name)|psi-mi:YJU2(display_short)|uniprotkb:CWC16(gene name synonym) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616364|imex:IM-25038-3 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - refseq:NP_012828.1|sgd:S000001578|mint:P28320|dip:DIP-1915N|ensemblfungi:YKL095W(gene)|ensemblfungi:YKL095W_mRNA(transcript)|go:"GO:0000349"(generation of catalytic spliceosome for first transesterification step)|go:"GO:0000350"(generation of catalytic spliceosome for second transesterification step)|go:"GO:0005634"(nucleus)|go:"GO:0008380"(RNA splicing)|go:"GO:0030620"(U2 snRNA binding)|go:"GO:0046872"(metal ion binding)|go:"GO:0071006"(U2-type catalytic step 1 spliceosome)|go:"GO:0071007"(U2-type catalytic step 2 spliceosome)|interpro:IPR007590(Protein of unknown function DUF572)|interpro:IPR043701|rcsb pdb:5GMK|rcsb pdb:5LJ3|rcsb pdb:5LJ5|rcsb pdb:5Y88|rcsb pdb:6EXN|rcsb pdb:6J6Q - - crc64:B57F3A173A2113FD figure legend:Table 1, Suppl Fig4|comment:The spiceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate C complex 2mM ATP was added and a deletion mutant of actin premRNA was used|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2017/12/02 - rogid:RB1yjFkujiRVEYwnEtk3x0Beo88559292 - false 32p radiolabel:?-?|tag:1-1|mutation:1-308 - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:P40567 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-789|uniprotkb:D6VVT9|ensemblfungi:YIR009W psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:msl1_yeast(display_long)|uniprotkb:YIB9W(orf name)|uniprotkb:YIR009W(locus name)|uniprotkb:MSL1(gene name)|psi-mi:MSL1(display_short) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616364|imex:IM-25038-3 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - refseq:NP_012274.1|sgd:S000001448|rcsb pdb:6BK8|ensemblfungi:YIR009W(gene)|ensemblfungi:YIR009W_mRNA(transcript)|go:"GO:0000398"(mRNA splicing, via spliceosome)|go:"GO:0005685"(U1 snRNP)|go:"GO:0030619"(U1 snRNA binding)|go:"GO:0030620"(U2 snRNA binding)|go:"GO:0071004"(U2-type prespliceosome)|interpro:IPR000504(RNA recognition motif, RNP-1)|interpro:IPR012677(Nucleotide-binding, alpha-beta plait)|interpro:IPR035979|rcsb pdb:5GMK|rcsb pdb:5LJ3|rcsb pdb:5LJ5|rcsb pdb:5MQ0|rcsb pdb:5NRL|rcsb pdb:5WSG|rcsb pdb:5Y88|rcsb pdb:5YLZ|rcsb pdb:5ZWM|rcsb pdb:5ZWO|dip:DIP-816N|mint:P40567|rcsb pdb:6EXN|rcsb pdb:6G90|rcsb pdb:6J6G|rcsb pdb:6J6H|rcsb pdb:6J6N|rcsb pdb:6J6Q - - - figure legend:Table 1, Suppl Fig4|comment:The spiceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate C complex 2mM ATP was added and a deletion mutant of actin premRNA was used|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2017/12/02 - rogid:GAiHZKcbfNjbem3+i1cZXFm4NfE559292 - false 32p radiolabel:?-?|tag:1-1|mutation:1-308 - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:P40565 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-25387|uniprotkb:D6VVT5|ensemblfungi:YIR005W psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:ist3_yeast(display_long)|uniprotkb:IST3(gene name)|psi-mi:IST3(display_short)|uniprotkb:SNU17(gene name synonym)|uniprotkb:YIR005W(locus name)|uniprotkb:YIB5W(orf name)|uniprotkb:U2 snRNP protein SNU17(gene name synonym)|uniprotkb:Increased sodium tolerance protein 3(gene name synonym) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616364|imex:IM-25038-3 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - refseq:NP_012270.1|sgd:S000001444|dip:DIP-2076N|ensemblfungi:YIR005W(gene)|ensemblfungi:YIR005W_mRNA(transcript)|go:"GO:0000245"(spliceosomal complex assembly)|go:"GO:0000349"(generation of catalytic spliceosome for first transesterification step)|go:"GO:0000398"(mRNA splicing, via spliceosome)|go:"GO:0003723"(RNA binding)|go:"GO:0005686"(U2 snRNP)|go:"GO:0005737"(cytoplasm)|go:"GO:0006406"(mRNA export from nucleus)|go:"GO:0070274"(RES complex)|go:"GO:0071011"(precatalytic spliceosome)|interpro:IPR000504(RNA recognition motif, RNP-1)|interpro:IPR012677(Nucleotide-binding, alpha-beta plait)|interpro:IPR035979|rcsb pdb:2MKC|rcsb pdb:2MY2|rcsb pdb:2MY3|rcsb pdb:4UQT|rcsb pdb:5GM6|rcsb pdb:5LQW|rcsb pdb:5ZWM|rcsb pdb:5ZWO - - crc64:9D51C3043AB823D2 figure legend:Table 1, Suppl Fig4|comment:The spiceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate C complex 2mM ATP was added and a deletion mutant of actin premRNA was used|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2017/12/02 - rogid:UlibOhZ4aPt5J8Ptg+Z1zA1VJXs559292 - false 32p radiolabel:?-?|tag:1-1|mutation:1-308 - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:P33334 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-465|uniprotkb:D3DLB4|ensemblfungi:YHR165C psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:prp8_yeast(display_long)|uniprotkb:PRP8(gene name)|psi-mi:PRP8(display_short)|uniprotkb:DBF3(gene name synonym)|uniprotkb:DNA39(gene name synonym)|uniprotkb:RNA8(gene name synonym)|uniprotkb:SLT21(gene name synonym)|uniprotkb:USA2(gene name synonym)|uniprotkb:YHR165C(locus name) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616364|imex:IM-25038-3 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - refseq:NP_012035.1|sgd:S000001208|rcsb pdb:4ILJ|rcsb pdb:5DCA|rcsb pdb:5GAM|rcsb pdb:5GAN|rcsb pdb:5GAO|rcsb pdb:5GAP|rcsb pdb:5GM6|rcsb pdb:5GMK|rcsb pdb:5LJ3|rcsb pdb:5LJ5|rcsb pdb:5LQW|rcsb pdb:5M52|rcsb pdb:5M5P|rcsb pdb:5MPS|rcsb pdb:5MQ0|rcsb pdb:5NRL|rcsb pdb:5QY1|rcsb pdb:5QY2|rcsb pdb:5QY3|rcsb pdb:5QY4|rcsb pdb:5QY5|rcsb pdb:5QY6|rcsb pdb:5QY7|rcsb pdb:5QY8|rcsb pdb:5QY9|rcsb pdb:5QYA|rcsb pdb:5QYB|rcsb pdb:5QYC|rcsb pdb:5QYD|rcsb pdb:5QYE|rcsb pdb:5QYF|rcsb pdb:5QYG|rcsb pdb:5QYH|rcsb pdb:5QYI|rcsb pdb:5QYJ|rcsb pdb:5QYK|rcsb pdb:5QYL|rcsb pdb:5QYM|rcsb pdb:5QYN|rcsb pdb:5QYO|rcsb pdb:5QYP|rcsb pdb:5QYQ|rcsb pdb:5QYR|rcsb pdb:5QYS|rcsb pdb:5QYT|rcsb pdb:5QYU|rcsb pdb:5QYV|rcsb pdb:5QYW|rcsb pdb:5QYX|rcsb pdb:5QYY|rcsb pdb:5QYZ|rcsb pdb:5QZ0|rcsb pdb:5QZ1|rcsb pdb:5QZ2|rcsb pdb:5QZ3|rcsb pdb:5QZ4|rcsb pdb:5QZ5|rcsb pdb:5QZ6|rcsb pdb:5QZ7|rcsb pdb:5QZ8|rcsb pdb:5QZ9|rcsb pdb:5QZA|rcsb pdb:5QZB|rcsb pdb:5QZC|rcsb pdb:5QZD|rcsb pdb:5QZE|rcsb pdb:5QZF|rcsb pdb:5QZG|rcsb pdb:5QZH|rcsb pdb:5QZI|rcsb pdb:5QZJ|rcsb pdb:5QZK|rcsb pdb:5QZL|rcsb pdb:5QZM|rcsb pdb:5QZN|rcsb pdb:5QZO|rcsb pdb:5QZP|rcsb pdb:5R14|rcsb pdb:5R15|rcsb pdb:5R16|rcsb pdb:5R17|rcsb pdb:5R18|rcsb pdb:5R19|rcsb pdb:5R1A|rcsb pdb:5R1B|rcsb pdb:5R1C|rcsb pdb:5R1D|rcsb pdb:5R1E|rcsb pdb:5R1F|rcsb pdb:5R1G|rcsb pdb:5R1H|rcsb pdb:5R1I|rcsb pdb:5R1J|rcsb pdb:5R1K|rcsb pdb:5R1L|rcsb pdb:5R1M|rcsb pdb:5R1N|rcsb pdb:5R1O|rcsb pdb:5R1P|rcsb pdb:5R1Q|rcsb pdb:5R1S|rcsb pdb:5WSG|rcsb pdb:5Y88|rcsb pdb:5YLZ|rcsb pdb:5ZWM|rcsb pdb:5ZWO|rcsb pdb:6BK8|rcsb pdb:6EXN|rcsb pdb:6J6G|rcsb pdb:6J6H|rcsb pdb:6J6N|rcsb pdb:6J6Q|rcsb pdb:6TEO|rcsb pdb:7B9V|rcsb pdb:5QZQ|rcsb pdb:5QZR|rcsb pdb:5QZS|rcsb pdb:5QZT|rcsb pdb:5QZU|rcsb pdb:5QZV|rcsb pdb:5QZW|rcsb pdb:5QZX|rcsb pdb:5QZY|rcsb pdb:5QZZ|rcsb pdb:5R00|rcsb pdb:5R01|rcsb pdb:5R02|rcsb pdb:5R03|rcsb pdb:5R04|rcsb pdb:5R05|rcsb pdb:5R06|rcsb pdb:5R07|rcsb pdb:5R08|rcsb pdb:5R09|rcsb pdb:5R0A|rcsb pdb:5R0B|rcsb pdb:5R0C|rcsb pdb:5R0D|rcsb pdb:5R0E|rcsb pdb:5R0F|rcsb pdb:5R0G|rcsb pdb:5R0H|rcsb pdb:5R0I|rcsb pdb:5R0J|rcsb pdb:5R0K|rcsb pdb:5R0L|rcsb pdb:5R0M|rcsb pdb:5R0N|rcsb pdb:5R0O|rcsb pdb:5R0P|rcsb pdb:5R0Q|rcsb pdb:5R0R|rcsb pdb:5R0S|rcsb pdb:5R0T|rcsb pdb:5R0U|rcsb pdb:5R0V|rcsb pdb:5R0W|rcsb pdb:5R0X|rcsb pdb:5R0Y|rcsb pdb:5R0Z|rcsb pdb:5R10|rcsb pdb:5R11|rcsb pdb:5R12|rcsb pdb:5R13|ensemblfungi:YHR165C(gene)|ensemblfungi:YHR165C_mRNA(transcript)|go:"GO:0000244"(spliceosomal tri-snRNP complex assembly)|go:"GO:0000350"(generation of catalytic spliceosome for second transesterification step)|go:"GO:0000389"(mRNA 3'-splice site recognition)|go:"GO:0000395"(mRNA 5'-splice site recognition)|go:"GO:0003729"(mRNA binding)|go:"GO:0005634"(nucleus)|go:"GO:0005682"(U5 snRNP)|go:"GO:0008237"(metallopeptidase activity)|go:"GO:0017070"(U6 snRNA binding)|go:"GO:0030619"(U1 snRNA binding)|go:"GO:0030620"(U2 snRNA binding)|go:"GO:0030623"(U5 snRNA binding)|go:"GO:0046540"(U4/U6 x U5 tri-snRNP complex)|go:"GO:0070122"(isopeptidase activity)|go:"GO:0071013"(catalytic step 2 spliceosome)|rcsb pdb:3SBT|rcsb pdb:3ZEF|rcsb pdb:4BGD|rcsb pdb:4I43|go:"GO:0097157"(pre-mRNA intronic binding)|interpro:IPR000555(Mov34/MPN/PAD-1)|interpro:IPR012337(Polynucleotidyl transferase, Ribonuclease H fold)|interpro:IPR012591(PRO8NT)|interpro:IPR012592(PROCN)|interpro:IPR012984(PRO, C-terminal)|interpro:IPR019580|interpro:IPR019581|interpro:IPR019582|interpro:IPR021983|interpro:IPR027652|interpro:IPR037518|interpro:IPR042516|interpro:IPR043172|interpro:IPR043173|rcsb pdb:2OG4|rcsb pdb:3E66|rcsb pdb:3E9O|rcsb pdb:3E9P|rcsb pdb:3JCM|rcsb pdb:3SBG|rcsb pdb:4ILG|rcsb pdb:4ILH|dip:DIP-2427N|mint:P33334 - - crc64:8F4F6F89D34D3508 figure legend:Table 1, Suppl Fig4|comment:The spiceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate C complex 2mM ATP was added and a deletion mutant of actin premRNA was used|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2017/12/02 - rogid:0JoVp7ULlbib7N5+GOPksHStSmY559292 - false 32p radiolabel:?-?|tag:1-1|mutation:1-308 - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:P32639 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-861|uniprotkb:D3DM80|ensemblfungi:YER172C psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:brr2_yeast(display_long)|uniprotkb:BRR2(gene name)|psi-mi:BRR2(display_short)|uniprotkb:RSS1(gene name synonym)|uniprotkb:SNU246(gene name synonym)|uniprotkb:YER172C(locus name)|uniprotkb:SYGP-ORF66(orf name)|uniprotkb:Protein Snu246(gene name synonym) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616364|imex:IM-25038-3 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - refseq:NP_011099.1|sgd:S000000974|dip:DIP-850N|mint:P32639|ensemblfungi:YER172C(gene)|ensemblfungi:YER172C_mRNA(transcript)|go:"GO:0000388"("spliceosome conformational change to release U4 (or U4atac) and U1 (or U11)")|go:"GO:0003723"(RNA binding)|go:"GO:0003724"(RNA helicase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005634"(nucleus)|go:"GO:0005681"(spliceosomal complex)|go:"GO:0005682"(U5 snRNP)|go:"GO:0042802"(identical protein binding)|go:"GO:0046540"(U4/U6 x U5 tri-snRNP complex)|go:"GO:0140603"(obsolete ATP hydrolysis activity)|interpro:IPR001650(DNA/RNA helicase, C-terminal)|interpro:IPR004179(Sec63)|interpro:IPR011545(DNA/RNA helicase, DEAD/DEAH box type, N-terminal)|interpro:IPR014001(DEAD-like helicase, N-terminal)|interpro:IPR014756(Immunoglobulin E-set)|interpro:IPR027417|interpro:IPR035892|interpro:IPR036388|interpro:IPR036390|interpro:IPR041094|rcsb pdb:3HIB|rcsb pdb:3IM1|rcsb pdb:3IM2|rcsb pdb:3JCM|rcsb pdb:4BGD|rcsb pdb:5DCA|rcsb pdb:5GAN|rcsb pdb:5GAO|rcsb pdb:5GAP|rcsb pdb:5GM6|rcsb pdb:5LJ5|rcsb pdb:5LQW|rcsb pdb:5M52|rcsb pdb:5M5P|rcsb pdb:5NRL|rcsb pdb:5ZWM|rcsb pdb:5ZWO|rcsb pdb:7B9V - - crc64:DFAF7E3B7168D944 figure legend:Table 1, Suppl Fig4|comment:The spiceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate C complex 2mM ATP was added and a deletion mutant of actin premRNA was used|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2017/12/02 - rogid:2bNoVkou3QatkNCtPIiSdvPdgEo559292 - false 32p radiolabel:?-?|tag:1-1|mutation:1-308 - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:P40018 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-432|uniprotkb:D3DLS8|ensemblfungi:YER029C psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:rsmb_yeast(display_long)|uniprotkb:SMB1(gene name)|psi-mi:SMB1(display_short)|uniprotkb:Sm protein B(gene name synonym)|uniprotkb:YER029C(locus name) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616364|imex:IM-25038-3 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - sgd:S000000831|rcsb pdb:5MPS|rcsb pdb:5MQ0|rcsb pdb:5NRL|rcsb pdb:5WSG|rcsb pdb:5Y88|rcsb pdb:5YLZ|rcsb pdb:5ZWM|rcsb pdb:5ZWN|rcsb pdb:5ZWO|rcsb pdb:6BK8|rcsb pdb:6EXN|rcsb pdb:6G90|rcsb pdb:6J6G|rcsb pdb:6J6H|rcsb pdb:6J6N|rcsb pdb:6J6Q|rcsb pdb:6N7P|rcsb pdb:6N7R|rcsb pdb:6N7X|rcsb pdb:7B9V|ensemblfungi:YER029C(gene)|ensemblfungi:YER029C_mRNA(transcript)|go:"GO:0000398"(mRNA splicing, via spliceosome)|go:"GO:0003723"(RNA binding)|go:"GO:0005682"(U5 snRNP)|go:"GO:0005685"(U1 snRNP)|go:"GO:0005686"(U2 snRNP)|go:"GO:0005687"(U4 snRNP)|go:"GO:0005737"(cytoplasm)|go:"GO:0046540"(U4/U6 x U5 tri-snRNP complex)|go:"GO:0071004"(U2-type prespliceosome)|go:"GO:0071013"(catalytic step 2 spliceosome)|interpro:IPR001163(Like-Sm ribonucleoprotein, core)|interpro:IPR010920(Like-Sm ribonucleoprotein-related, core)|rcsb pdb:3JCM|rcsb pdb:5GAM|rcsb pdb:5GAN|rcsb pdb:5GAO|rcsb pdb:5GM6|refseq:NP_010946.3|rcsb pdb:5GMK|rcsb pdb:5LJ3|rcsb pdb:5LJ5|rcsb pdb:5LQW|dip:DIP-843N|mint:P40018 - - crc64:0A1078D5AA241430 figure legend:Table 1, Suppl Fig4|comment:The spiceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate C complex 2mM ATP was added and a deletion mutant of actin premRNA was used|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2017/12/02 - rogid:b62+dBOdvQgeE0oup4kbXbviS1U559292 - false 32p radiolabel:?-?|tag:1-1|mutation:1-308 - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:P24384 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-13853|ensemblfungi:YER013W|uniprotkb:D3DLR0 psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:prp22_yeast(display_long)|uniprotkb:PRP22(gene name)|psi-mi:PRP22(display_short)|uniprotkb:YER013W(locus name) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616364|imex:IM-25038-3 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - sgd:S000000815|go:"GO:0000390"(spliceosomal complex disassembly)|ensemblfungi:YER013W(gene)|go:"GO:0003723"(RNA binding)|ensemblfungi:YER013W_mRNA(transcript)|go:"GO:0000350"(generation of catalytic spliceosome for second transesterification step)|go:"GO:0003724"(RNA helicase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0071007"(U2-type catalytic step 2 spliceosome)|go:"GO:0071013"(catalytic step 2 spliceosome)|go:"GO:0071021"(U2-type post-spliceosomal complex)|go:"GO:0140603"(obsolete ATP hydrolysis activity)|interpro:IPR001650(DNA/RNA helicase, C-terminal)|interpro:IPR002464(DNA/RNA helicase, ATP-dependent, DEAH-box type, conserved site)|interpro:IPR003029(S1, RNA binding)|interpro:IPR007502(Helicase-associated region)|interpro:IPR011709(Region of unknown function DUF1605)|interpro:IPR012340(Nucleic acid-binding, OB-fold)|interpro:IPR014001(DEAD-like helicase, N-terminal)|interpro:IPR022967|interpro:IPR027417|interpro:IPR044762|rcsb pdb:5YLZ|rcsb pdb:6BK8|rcsb pdb:6EXN|rcsb pdb:5MQ0|refseq:NP_010929.3|mint:P24384|dip:DIP-655N - - crc64:D92DBD620531B9A7 figure legend:Table 1, Suppl Fig4|comment:The spiceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate C complex 2mM ATP was added and a deletion mutant of actin premRNA was used|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2017/12/02 - rogid:iDMheQvPn9vaU/Fect6WlVsApZM559292 - false 32p radiolabel:?-?|tag:1-1|mutation:1-308 - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:P25337 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-547|ensemblfungi:YCR063W|uniprotkb:D6VR67 psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:bud31_yeast(display_long)|uniprotkb:BUD31(gene name)|psi-mi:BUD31(display_short)|uniprotkb:Bud site selection protein 31(gene name synonym)|uniprotkb:CWC14(gene name synonym)|uniprotkb:Complexed with CEF1 protein 14(gene name synonym)|uniprotkb:YCR63W(orf name)|uniprotkb:YCR903(orf name)|uniprotkb:YCR063W(locus name) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616364|imex:IM-25038-3 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - refseq:NP_009990.1|sgd:S000000659|go:"GO:0000398"(mRNA splicing, via spliceosome)|go:"GO:0005634"(nucleus)|go:"GO:0005681"(spliceosomal complex)|go:"GO:0005686"(U2 snRNP)|interpro:IPR001748(G10 protein)|interpro:IPR018230|rcsb pdb:2MY1|ensemblfungi:YCR063W(gene)|ensemblfungi:YCR063W_mRNA(transcript)|go:"GO:0000282"(cellular bud site selection)|rcsb pdb:5GM6|rcsb pdb:5GMK|rcsb pdb:5LJ3|rcsb pdb:5LJ5|rcsb pdb:5LQW|rcsb pdb:5MPS|rcsb pdb:5MQ0|rcsb pdb:5WSG|rcsb pdb:5Y88|rcsb pdb:5YLZ|rcsb pdb:6BK8|rcsb pdb:6EXN|rcsb pdb:6J6G|rcsb pdb:6J6H|rcsb pdb:6J6N|rcsb pdb:6J6Q|dip:DIP-1180N|mint:P25337 - - crc64:70A8B15F8DA6F85D figure legend:Table 1, Suppl Fig4|comment:The spiceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate C complex 2mM ATP was added and a deletion mutant of actin premRNA was used|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2017/12/02 - rogid:DImrf7jgUrSuQtfiOvBeJr5Cl/w559292 - false 32p radiolabel:?-?|tag:1-1|mutation:1-308 - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:P38302 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-20921|uniprotkb:D6VQI3|ensemblfungi:YBR188C psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:ntc20_yeast(display_long)|uniprotkb:YBR188C(locus name)|uniprotkb:YBR1316(orf name)|uniprotkb:NTC20(gene name)|psi-mi:NTC20(display_short)|uniprotkb:SNT384(gene name synonym)|uniprotkb:PRP19-associated complex protein 20(gene name synonym)|uniprotkb:Synergistic to PRP19 mutation protein 384(gene name synonym) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616364|imex:IM-25038-3 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - refseq:NP_009747.3|sgd:S000000392|mint:P38302|ensemblfungi:YBR188C(gene)|ensemblfungi:YBR188C_mRNA(transcript)|go:"GO:0000398"(mRNA splicing, via spliceosome)|go:"GO:0000974"(Prp19 complex)|go:"GO:0071006"(U2-type catalytic step 1 spliceosome)|dip:DIP-1683N - - crc64:15C5234B25213223 figure legend:Table 1, Suppl Fig4|comment:The spiceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate C complex 2mM ATP was added and a deletion mutant of actin premRNA was used|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2017/12/02 - rogid:Ucc4R0lhhhAQfrsXvs+BtCnuJQg559292 - false 32p radiolabel:?-?|tag:1-1|mutation:1-308 - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:P38241 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-780|uniprotkb:P89497|uniprotkb:D6VQ64|ensemblfungi:YBR065C psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:slt11_yeast(display_long)|uniprotkb:ECM2(gene name)|psi-mi:ECM2(display_short)|uniprotkb:SLT11(gene name synonym)|uniprotkb:YBR065C(locus name)|uniprotkb:YBR0614(orf name)|uniprotkb:Synthetic lethality with U2 protein 11(gene name synonym)|uniprotkb:Extracellular mutant protein 2(gene name synonym) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616364|imex:IM-25038-3 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - sgd:S000000269|refseq:NP_009621.3|dip:DIP-5359N|mint:P38241|ensemblfungi:YBR065C(gene)|ensemblfungi:YBR065C_mRNA(transcript)|go:"GO:0000398"(mRNA splicing, via spliceosome)|go:"GO:0000974"(Prp19 complex)|go:"GO:0005634"(nucleus)|go:"GO:0017070"(U6 snRNA binding)|go:"GO:0036002"(pre-mRNA binding)|go:"GO:0071006"(U2-type catalytic step 1 spliceosome)|go:"GO:0071007"(U2-type catalytic step 2 spliceosome)|interpro:IPR034356|interpro:IPR039171|rcsb pdb:5GM6|rcsb pdb:5GMK|rcsb pdb:5LJ3|rcsb pdb:5LJ5|rcsb pdb:5LQW|rcsb pdb:5MPS|rcsb pdb:5MQ0|rcsb pdb:5WSG|rcsb pdb:5Y88|rcsb pdb:5YLZ|rcsb pdb:6BK8|rcsb pdb:6EXN|rcsb pdb:6J6G|rcsb pdb:6J6H|rcsb pdb:6J6N|rcsb pdb:6J6Q - - crc64:BF21E687817A42B4 figure legend:Table 1, Suppl Fig4|comment:The spiceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate C complex 2mM ATP was added and a deletion mutant of actin premRNA was used|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2017/12/02 - rogid:ioGlVp9msutMpWXME0tICQn8GDQ559292 - false 32p radiolabel:?-?|tag:1-1|mutation:1-308 - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:P19735 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-227|uniprotkb:D6VQ54|ensemblfungi:YBR055C psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:prp6_yeast(display_long)|uniprotkb:PRP6(gene name)|psi-mi:PRP6(display_short)|uniprotkb:RNA6(gene name synonym)|uniprotkb:YBR055C(locus name)|uniprotkb:YBR0508(orf name) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616364|imex:IM-25038-3 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - refseq:NP_009611.1|sgd:S000000259|mint:P19735|dip:DIP-1290N|ensemblfungi:YBR055C(gene)|ensemblfungi:YBR055C_mRNA(transcript)|go:"GO:0000244"(spliceosomal tri-snRNP complex assembly)|go:"GO:0000398"(mRNA splicing, via spliceosome)|go:"GO:0046540"(U4/U6 x U5 tri-snRNP complex)|go:"GO:0071001"(U4/U6 snRNP)|go:"GO:0071013"(catalytic step 2 spliceosome)|interpro:IPR003107(RNA-processing protein, HAT helix)|interpro:IPR010491(PRP1 splicing factor, N-terminal)|interpro:IPR011990(Tetratricopeptide-like helical)|interpro:IPR019734|interpro:IPR027108|rcsb pdb:3JCM|rcsb pdb:5GAN|rcsb pdb:5GAP|rcsb pdb:5NRL|rcsb pdb:5ZWM|rcsb pdb:5ZWO - - crc64:3E87F8AF63EAAC41 figure legend:Table 1, Suppl Fig4|comment:The spiceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate C complex 2mM ATP was added and a deletion mutant of actin premRNA was used|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2017/12/02 - rogid:v3xgQmlk5z/P4uu09sipcLZmlpE559292 - false 32p radiolabel:?-?|tag:1-1|mutation:1-308 - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 uniprotkb:P28004 ensembl:YFL039C.1|sgd:YFL039C intact:EBI-20640|uniprotkb:D6VPI6|ensemblfungi:YAL032C psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:prp45_yeast(display_long)|uniprotkb:YAL032C(locus name)|uniprotkb:PRP45(gene name)|psi-mi:PRP45(display_short)|uniprotkb:FUN20(gene name synonym) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616364|imex:IM-25038-3 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0326"(protein) - refseq:NP_009370.1|sgd:S000000030|ensemblfungi:YAL032C(gene)|ensemblfungi:YAL032C_mRNA(transcript)|go:"GO:0000350"(generation of catalytic spliceosome for second transesterification step)|go:"GO:0005634"(nucleus)|go:"GO:0071006"(U2-type catalytic step 1 spliceosome)|go:"GO:0071007"(U2-type catalytic step 2 spliceosome)|go:"GO:0071014"(post-mRNA release spliceosomal complex)|interpro:IPR004015(SKIP/SNW)|interpro:IPR017862|rcsb pdb:5GM6|rcsb pdb:5GMK|rcsb pdb:5LJ3|rcsb pdb:5LJ5|rcsb pdb:5LQW|rcsb pdb:5MPS|rcsb pdb:5MQ0|rcsb pdb:5WSG|rcsb pdb:5Y88|rcsb pdb:5YLZ|rcsb pdb:6BK8|rcsb pdb:6EXN|rcsb pdb:6J6G|rcsb pdb:6J6H|rcsb pdb:6J6N|rcsb pdb:6J6Q|dip:DIP-2774N|mint:P28004 - - crc64:FE5A724186D7FE84 figure legend:Table 1, Suppl Fig4|comment:The spiceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate C complex 2mM ATP was added and a deletion mutant of actin premRNA was used|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2017/12/02 - rogid:qoHF1UsO3k+JOTOfigSuy2P84/k559292 - false 32p radiolabel:?-?|tag:1-1|mutation:1-308 - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) intact:EBI-11413715 intact:EBI-10824938 ensembl:YFL039C.1|sgd:YFL039C rnacentral:URS00006184A9_559292 psi-mi:premrna actin yeast(display_short)|psi-mi:EBI-11413715(display_long) psi-mi:snru6_yeast(display_short)|psi-mi:EBI-10824938(display_long) psi-mi:"MI:0096"(pull down) Fabrizio et al. (2009) imex:IM-25038|pubmed:19941820 taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae) psi-mi:"MI:0914"(association) psi-mi:"MI:0469"(IntAct) intact:EBI-11616364|imex:IM-25038-3 - psi-mi:"MI:1060"(spoke expansion) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0499"(unspecified role) psi-mi:"MI:0496"(bait) psi-mi:"MI:0498"(prey) psi-mi:"MI:0320"(ribonucleic acid) psi-mi:"MI:0607"(small nuclear rna) - - - - - figure legend:Table 1, Suppl Fig4|comment:The spiceosome was affinity purified with the actin mRNA and separated on glycerol gradient. To isolate C complex 2mM ATP was added and a deletion mutant of actin premRNA was used|full coverage:Only protein-protein interactions|curation depth:imex curation taxid:-1(in vitro)|taxid:-1(In vitro) - 2015/11/11 2017/12/02 - - - false 32p radiolabel:?-?|tag:1-1|mutation:1-308 - - - psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry) psi-mi:"MI:0032"(de novo protein sequencing by mass spectrometry)