#ID(s) interactor A	ID(s) interactor B	Alt. ID(s) interactor A	Alt. ID(s) interactor B	Alias(es) interactor A	Alias(es) interactor B	Interaction detection method(s)	Publication 1st author(s)	Publication Identifier(s)	Taxid interactor A	Taxid interactor B	Interaction type(s)	Source database(s)	Interaction identifier(s)	Confidence value(s)	Expansion method(s)	Biological role(s) interactor A	Biological role(s) interactor B	Experimental role(s) interactor A	Experimental role(s) interactor B	Type(s) interactor A	Type(s) interactor B	Xref(s) interactor A	Xref(s) interactor B	Interaction Xref(s)	Annotation(s) interactor A	Annotation(s) interactor B	Interaction annotation(s)	Host organism(s)	Interaction parameter(s)	Creation date	Update date	Checksum(s) interactor A	Checksum(s) interactor B	Interaction Checksum(s)	Negative	Feature(s) interactor A	Feature(s) interactor B	Stoichiometry(s) interactor A	Stoichiometry(s) interactor B	Identification method participant A	Identification method participant B
uniprotkb:Q99697-3	intact:EBI-9847176	intact:EBI-1175243|ensembl:ENSP00000347192|ensembl:ENSP00000484909	-	psi-mi:q99697-3(display_long)|uniprotkb:ARP1A(isoform synonym)|uniprotkb:PITX2(gene name)|psi-mi:PITX2(display_short)|uniprotkb:ARP1(gene name synonym)|uniprotkb:RGS(gene name synonym)|uniprotkb:RIEG(gene name synonym)|uniprotkb:RIEG1(gene name synonym)|uniprotkb:ALL1-responsive protein ARP1(gene name synonym)|uniprotkb:Homeobox protein PITX2(gene name synonym)|uniprotkb:Paired-like homeodomain transcription factor 2(gene name synonym)|uniprotkb:RIEG bicoid-related homeobox transcription factor(gene name synonym)|uniprotkb:Solurshin(gene name synonym)	psi-mi:bcd_probe(display_short)|psi-mi:EBI-9847176(display_long)	psi-mi:"MI:0413"(electrophoretic mobility shift assay)	Amendt et al. (1998)	pubmed:9685346|imex:IM-23271	taxid:9606(human)|taxid:9606(Homo sapiens)	taxid:32630(synthetic construct)|taxid:32630(synthetic construct)	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:1332"(bhf-ucl)	intact:EBI-9847112|imex:IM-23271-4	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0680"(single stranded deoxyribonucleic acid)	intact:EBI-1175211(pitx2_human)|ensembl:ENSG00000164093(gene)|ensembl:ENST00000355080(transcript)|ensembl:ENST00000616641(transcript)	-	-	isoform-comment:"Variant in position: 58:F->L (in ASGD4)."	-	figure legend:Fig. 1C, 2A, 2C, 6|agonist:"Pit-1  (P10037)"|dataset:Cardiac - Interactions involving cardiac related proteins|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	kd:50.0x10^-9(molar)	2014/10/13	2014/11/10	rogid:lbwqpXFBKq9v3kQyno+c46b48Wo9606	-	intact-crc:DA0B14D379EAD779	false	-	32p radiolabel:?-?	-	-	psi-mi:"MI:0833"(autoradiography)	psi-mi:"MI:0833"(autoradiography)
intact:EBI-9847176	uniprotkb:Q99697-3	-	intact:EBI-1175243|ensembl:ENSP00000347192|ensembl:ENSP00000484909	psi-mi:bcd_probe(display_short)|psi-mi:EBI-9847176(display_long)	psi-mi:q99697-3(display_long)|uniprotkb:ARP1A(isoform synonym)|uniprotkb:PITX2(gene name)|psi-mi:PITX2(display_short)|uniprotkb:ARP1(gene name synonym)|uniprotkb:RGS(gene name synonym)|uniprotkb:RIEG(gene name synonym)|uniprotkb:RIEG1(gene name synonym)|uniprotkb:ALL1-responsive protein ARP1(gene name synonym)|uniprotkb:Homeobox protein PITX2(gene name synonym)|uniprotkb:Paired-like homeodomain transcription factor 2(gene name synonym)|uniprotkb:RIEG bicoid-related homeobox transcription factor(gene name synonym)|uniprotkb:Solurshin(gene name synonym)	psi-mi:"MI:0413"(electrophoretic mobility shift assay)	Amendt et al. (1998)	pubmed:9685346|imex:IM-23271	taxid:32630(synthetic construct)|taxid:32630(synthetic construct)	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:1332"(bhf-ucl)	intact:EBI-9867920|imex:IM-23271-2	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0680"(single stranded deoxyribonucleic acid)	psi-mi:"MI:0326"(protein)	-	intact:EBI-1175211(pitx2_human)|ensembl:ENSG00000164093(gene)|ensembl:ENST00000355080(transcript)|ensembl:ENST00000616641(transcript)	-	-	isoform-comment:"Variant in position: 58:F->L (in ASGD4)."	figure legend:Fig. 6|comment:Pit-1 increased the amount of Pitx2-DNA bound complex approximately 2–3-fold.  The Pit-1-enhanced binding of Pitx2 was competed by unlabeled bicoid DNA|dataset:Cardiac - Interactions involving cardiac related proteins|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2014/10/13	2014/11/10	-	rogid:lbwqpXFBKq9v3kQyno+c46b48Wo9606	intact-crc:423444F907E0BAF0	false	32p radiolabel:?-?	-	-	-	psi-mi:"MI:0833"(autoradiography)	psi-mi:"MI:0833"(autoradiography)
intact:EBI-9847176	uniprotkb:P10037	-	intact:EBI-9825525|uniprotkb:Q63431|ensembl:ENSRNOP00000000905	psi-mi:bcd_probe(display_short)|psi-mi:EBI-9847176(display_long)	psi-mi:pit1_rat(display_long)|uniprotkb:Pou1f1(gene name)|psi-mi:Pou1f1(display_short)|uniprotkb:Ghf-1(gene name synonym)|uniprotkb:Pit-1(gene name synonym)|uniprotkb:Pit1(gene name synonym)|uniprotkb:Growth hormone factor 1(gene name synonym)	psi-mi:"MI:0413"(electrophoretic mobility shift assay)	Amendt et al. (1998)	pubmed:9685346|imex:IM-23271	taxid:32630(synthetic construct)|taxid:32630(synthetic construct)	taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)")	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:1332"(bhf-ucl)	intact:EBI-9867920|imex:IM-23271-2	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0840"(stimulator)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0680"(single stranded deoxyribonucleic acid)	psi-mi:"MI:0326"(protein)	-	rgd:3367|refseq:XP_006248036.1|ensembl:ENSRNOG00000000715(gene)|ensembl:ENSRNOT00000000905(transcript)|go:"GO:0000122"(negative regulation of transcription by RNA polymerase II)|go:"GO:0000978"(RNA polymerase II cis-regulatory region sequence-specific DNA binding)|go:"GO:0000981"(DNA-binding transcription factor activity, RNA polymerase II-specific)|go:"GO:0001228"(DNA-binding transcription activator activity, RNA polymerase II-specific)|go:"GO:0001708"(cell fate specification)|go:"GO:0003677"(DNA binding)|go:"GO:0003682"(chromatin binding)|go:"GO:0003700"(DNA-binding transcription factor activity)|go:"GO:0005634"(nucleus)|go:"GO:0005654"(nucleoplasm)|go:"GO:0005829"(cytosol)|go:"GO:0006355"(regulation of transcription, DNA-templated)|go:"GO:0006357"(regulation of transcription by RNA polymerase II)|go:"GO:0008284"(positive regulation of cell population proliferation)|go:"GO:0008340"(determination of adult lifespan)|go:"GO:0021983"(pituitary gland development)|go:"GO:0021984"(adenohypophysis development)|go:"GO:0030183"(B cell differentiation)|go:"GO:0032962"(positive regulation of inositol trisphosphate biosynthetic process)|go:"GO:0040018"(positive regulation of multicellular organism growth)|go:"GO:0043565"(sequence-specific DNA binding)|go:"GO:0043567"(regulation of insulin-like growth factor receptor signaling pathway)|go:"GO:0045893"(positive regulation of transcription, DNA-templated)|go:"GO:0045944"(positive regulation of transcription by RNA polymerase II)|go:"GO:0060126"(somatotropin secreting cell differentiation)|go:"GO:0060133"(somatotropin secreting cell development)|go:"GO:0061629"(RNA polymerase II-specific DNA-binding transcription factor binding)|go:"GO:1990837"(sequence-specific double-stranded DNA binding)|interpro:IPR000327(POU-specific)|interpro:IPR001356(Homeobox)|interpro:IPR009057(Homeodomain-like)|interpro:IPR010982(Lambda repressor-like, DNA-binding)|interpro:IPR013847(POU)|interpro:IPR015586(Pituitary-specific positive transcription factor 1)|interpro:IPR017970|rcsb pdb:1AU7	-	-	-	figure legend:Fig. 6|comment:Pit-1 increased the amount of Pitx2-DNA bound complex approximately 2–3-fold.  The Pit-1-enhanced binding of Pitx2 was competed by unlabeled bicoid DNA|dataset:Cardiac - Interactions involving cardiac related proteins|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2014/10/13	2014/11/10	-	rogid:qGfEZ60egM4JNpLKiVLMXKgLphU10116	intact-crc:423444F907E0BAF0	false	32p radiolabel:?-?	-	-	2	psi-mi:"MI:0833"(autoradiography)	psi-mi:"MI:0833"(autoradiography)
intact:EBI-9847176	uniprotkb:P10037	-	intact:EBI-9825525|uniprotkb:Q63431|ensembl:ENSRNOP00000000905	psi-mi:bcd_probe(display_short)|psi-mi:EBI-9847176(display_long)	psi-mi:pit1_rat(display_long)|uniprotkb:Pou1f1(gene name)|psi-mi:Pou1f1(display_short)|uniprotkb:Ghf-1(gene name synonym)|uniprotkb:Pit-1(gene name synonym)|uniprotkb:Pit1(gene name synonym)|uniprotkb:Growth hormone factor 1(gene name synonym)	psi-mi:"MI:0413"(electrophoretic mobility shift assay)	Amendt et al. (1998)	pubmed:9685346|imex:IM-23271	taxid:32630(synthetic construct)|taxid:32630(synthetic construct)	taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)")	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:1332"(bhf-ucl)	intact:EBI-9974116|imex:IM-23271-3	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0840"(stimulator)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0680"(single stranded deoxyribonucleic acid)	psi-mi:"MI:0326"(protein)	-	rgd:3367|refseq:XP_006248036.1|ensembl:ENSRNOG00000000715(gene)|ensembl:ENSRNOT00000000905(transcript)|go:"GO:0000122"(negative regulation of transcription by RNA polymerase II)|go:"GO:0000978"(RNA polymerase II cis-regulatory region sequence-specific DNA binding)|go:"GO:0000981"(DNA-binding transcription factor activity, RNA polymerase II-specific)|go:"GO:0001228"(DNA-binding transcription activator activity, RNA polymerase II-specific)|go:"GO:0001708"(cell fate specification)|go:"GO:0003677"(DNA binding)|go:"GO:0003682"(chromatin binding)|go:"GO:0003700"(DNA-binding transcription factor activity)|go:"GO:0005634"(nucleus)|go:"GO:0005654"(nucleoplasm)|go:"GO:0005829"(cytosol)|go:"GO:0006355"(regulation of transcription, DNA-templated)|go:"GO:0006357"(regulation of transcription by RNA polymerase II)|go:"GO:0008284"(positive regulation of cell population proliferation)|go:"GO:0008340"(determination of adult lifespan)|go:"GO:0021983"(pituitary gland development)|go:"GO:0021984"(adenohypophysis development)|go:"GO:0030183"(B cell differentiation)|go:"GO:0032962"(positive regulation of inositol trisphosphate biosynthetic process)|go:"GO:0040018"(positive regulation of multicellular organism growth)|go:"GO:0043565"(sequence-specific DNA binding)|go:"GO:0043567"(regulation of insulin-like growth factor receptor signaling pathway)|go:"GO:0045893"(positive regulation of transcription, DNA-templated)|go:"GO:0045944"(positive regulation of transcription by RNA polymerase II)|go:"GO:0060126"(somatotropin secreting cell differentiation)|go:"GO:0060133"(somatotropin secreting cell development)|go:"GO:0061629"(RNA polymerase II-specific DNA-binding transcription factor binding)|go:"GO:1990837"(sequence-specific double-stranded DNA binding)|interpro:IPR000327(POU-specific)|interpro:IPR001356(Homeobox)|interpro:IPR009057(Homeodomain-like)|interpro:IPR010982(Lambda repressor-like, DNA-binding)|interpro:IPR013847(POU)|interpro:IPR015586(Pituitary-specific positive transcription factor 1)|interpro:IPR017970|rcsb pdb:1AU7	-	-	-	figure legend:Fig. 6|comment:Pit-1 had a 2-3 fold stimulatory effect on the binding of the mutant  Pitx2 T68P to the bicoid probe.|dataset:Cardiac - Interactions involving cardiac related proteins|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2014/10/13	2014/11/10	-	rogid:qGfEZ60egM4JNpLKiVLMXKgLphU10116	intact-crc:10335537CF22AA7B	false	32p radiolabel:?-?	-	-	2	psi-mi:"MI:0833"(autoradiography)	psi-mi:"MI:0833"(autoradiography)
intact:EBI-9847176	uniprotkb:Q99697-3	-	intact:EBI-1175243|ensembl:ENSP00000347192|ensembl:ENSP00000484909	psi-mi:bcd_probe(display_short)|psi-mi:EBI-9847176(display_long)	psi-mi:q99697-3(display_long)|uniprotkb:ARP1A(isoform synonym)|uniprotkb:PITX2(gene name)|psi-mi:PITX2(display_short)|uniprotkb:ARP1(gene name synonym)|uniprotkb:RGS(gene name synonym)|uniprotkb:RIEG(gene name synonym)|uniprotkb:RIEG1(gene name synonym)|uniprotkb:ALL1-responsive protein ARP1(gene name synonym)|uniprotkb:Homeobox protein PITX2(gene name synonym)|uniprotkb:Paired-like homeodomain transcription factor 2(gene name synonym)|uniprotkb:RIEG bicoid-related homeobox transcription factor(gene name synonym)|uniprotkb:Solurshin(gene name synonym)	psi-mi:"MI:0413"(electrophoretic mobility shift assay)	Amendt et al. (1998)	pubmed:9685346|imex:IM-23271	taxid:32630(synthetic construct)|taxid:32630(synthetic construct)	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:1332"(bhf-ucl)	intact:EBI-9974116|imex:IM-23271-3	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0680"(single stranded deoxyribonucleic acid)	psi-mi:"MI:0326"(protein)	-	intact:EBI-1175211(pitx2_human)|ensembl:ENSG00000164093(gene)|ensembl:ENST00000355080(transcript)|ensembl:ENST00000616641(transcript)	-	-	isoform-comment:"Variant in position: 58:F->L (in ASGD4)."	figure legend:Fig. 6|comment:Pit-1 had a 2-3 fold stimulatory effect on the binding of the mutant  Pitx2 T68P to the bicoid probe.|dataset:Cardiac - Interactions involving cardiac related proteins|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2014/10/13	2014/11/10	-	rogid:lbwqpXFBKq9v3kQyno+c46b48Wo9606	intact-crc:10335537CF22AA7B	false	32p radiolabel:?-?	mutation:68-68	-	-	psi-mi:"MI:0833"(autoradiography)	psi-mi:"MI:0833"(autoradiography)
intact:EBI-9847176	uniprotkb:Q99697-3	-	intact:EBI-1175243|ensembl:ENSP00000347192|ensembl:ENSP00000484909	psi-mi:bcd_probe(display_short)|psi-mi:EBI-9847176(display_long)	psi-mi:q99697-3(display_long)|uniprotkb:ARP1A(isoform synonym)|uniprotkb:PITX2(gene name)|psi-mi:PITX2(display_short)|uniprotkb:ARP1(gene name synonym)|uniprotkb:RGS(gene name synonym)|uniprotkb:RIEG(gene name synonym)|uniprotkb:RIEG1(gene name synonym)|uniprotkb:ALL1-responsive protein ARP1(gene name synonym)|uniprotkb:Homeobox protein PITX2(gene name synonym)|uniprotkb:Paired-like homeodomain transcription factor 2(gene name synonym)|uniprotkb:RIEG bicoid-related homeobox transcription factor(gene name synonym)|uniprotkb:Solurshin(gene name synonym)	psi-mi:"MI:0413"(electrophoretic mobility shift assay)	Amendt et al. (1998)	pubmed:9685346|imex:IM-23271	taxid:32630(synthetic construct)|taxid:32630(synthetic construct)	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:1332"(bhf-ucl)	intact:EBI-9867786|imex:IM-23271-9	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0680"(single stranded deoxyribonucleic acid)	psi-mi:"MI:0326"(protein)	-	intact:EBI-1175211(pitx2_human)|ensembl:ENSG00000164093(gene)|ensembl:ENST00000355080(transcript)|ensembl:ENST00000616641(transcript)	-	-	isoform-comment:"Variant in position: 58:F->L (in ASGD4)."	figure legend:Fig. 3A, 3B|comment:"The mutant Pitx2 T68P protein showed an overall increase in binding to all of the competitor DNAs (except the unlabeled bicoid DNA). The ability of these nonbicoid DNA competitors to bind Pitx2 T68P demonstrates a slight loss of binding specificity by this mutant."|dataset:Cardiac - Interactions involving cardiac related proteins|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2014/10/13	2014/11/10	-	rogid:lbwqpXFBKq9v3kQyno+c46b48Wo9606	intact-crc:EABB7E1EEB26B672	false	32p radiolabel:?-?	mutation:68-68	-	-	psi-mi:"MI:0833"(autoradiography)	psi-mi:"MI:0833"(autoradiography)
intact:EBI-9847176	intact:EBI-9847176	-	-	psi-mi:bcd_probe(display_short)|psi-mi:EBI-9847176(display_long)	psi-mi:bcd_probe(display_short)|psi-mi:EBI-9847176(display_long)	psi-mi:"MI:0413"(electrophoretic mobility shift assay)	Amendt et al. (1998)	pubmed:9685346|imex:IM-23271	taxid:32630(synthetic construct)|taxid:32630(synthetic construct)	taxid:32630(synthetic construct)|taxid:32630(synthetic construct)	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:1332"(bhf-ucl)	intact:EBI-9867786|imex:IM-23271-9	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0941"(competitor)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0680"(single stranded deoxyribonucleic acid)	psi-mi:"MI:0680"(single stranded deoxyribonucleic acid)	-	-	-	-	-	figure legend:Fig. 3A, 3B|comment:"The mutant Pitx2 T68P protein showed an overall increase in binding to all of the competitor DNAs (except the unlabeled bicoid DNA). The ability of these nonbicoid DNA competitors to bind Pitx2 T68P demonstrates a slight loss of binding specificity by this mutant."|dataset:Cardiac - Interactions involving cardiac related proteins|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2014/10/13	2014/11/10	-	-	intact-crc:EABB7E1EEB26B672	false	32p radiolabel:?-?	-	-	-	psi-mi:"MI:0833"(autoradiography)	psi-mi:"MI:0833"(autoradiography)
intact:EBI-9847176	uniprotkb:Q99697-3	-	intact:EBI-1175243|ensembl:ENSP00000347192|ensembl:ENSP00000484909	psi-mi:bcd_probe(display_short)|psi-mi:EBI-9847176(display_long)	psi-mi:q99697-3(display_long)|uniprotkb:ARP1A(isoform synonym)|uniprotkb:PITX2(gene name)|psi-mi:PITX2(display_short)|uniprotkb:ARP1(gene name synonym)|uniprotkb:RGS(gene name synonym)|uniprotkb:RIEG(gene name synonym)|uniprotkb:RIEG1(gene name synonym)|uniprotkb:ALL1-responsive protein ARP1(gene name synonym)|uniprotkb:Homeobox protein PITX2(gene name synonym)|uniprotkb:Paired-like homeodomain transcription factor 2(gene name synonym)|uniprotkb:RIEG bicoid-related homeobox transcription factor(gene name synonym)|uniprotkb:Solurshin(gene name synonym)	psi-mi:"MI:0413"(electrophoretic mobility shift assay)	Amendt et al. (1998)	pubmed:9685346|imex:IM-23271	taxid:32630(synthetic construct)|taxid:32630(synthetic construct)	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:1332"(bhf-ucl)	intact:EBI-9867762|imex:IM-23271-7	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0680"(single stranded deoxyribonucleic acid)	psi-mi:"MI:0326"(protein)	-	intact:EBI-1175211(pitx2_human)|ensembl:ENSG00000164093(gene)|ensembl:ENST00000355080(transcript)|ensembl:ENST00000616641(transcript)	-	-	isoform-comment:"Variant in position: 58:F->L (in ASGD4)."	figure legend:Fig. 2A, 2B|comment:"Unlabeled bicoid DNA (Bcd) caused more than 85% inhibition."|dataset:Cardiac - Interactions involving cardiac related proteins|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2014/10/13	2014/11/10	-	rogid:lbwqpXFBKq9v3kQyno+c46b48Wo9606	intact-crc:4D0AB241BB04D4B5	false	32p radiolabel:?-?	-	-	-	psi-mi:"MI:0833"(autoradiography)	psi-mi:"MI:0833"(autoradiography)
intact:EBI-9847176	intact:EBI-9847176	-	-	psi-mi:bcd_probe(display_short)|psi-mi:EBI-9847176(display_long)	psi-mi:bcd_probe(display_short)|psi-mi:EBI-9847176(display_long)	psi-mi:"MI:0413"(electrophoretic mobility shift assay)	Amendt et al. (1998)	pubmed:9685346|imex:IM-23271	taxid:32630(synthetic construct)|taxid:32630(synthetic construct)	taxid:32630(synthetic construct)|taxid:32630(synthetic construct)	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:1332"(bhf-ucl)	intact:EBI-9867762|imex:IM-23271-7	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0941"(competitor)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0680"(single stranded deoxyribonucleic acid)	psi-mi:"MI:0680"(single stranded deoxyribonucleic acid)	-	-	-	-	-	figure legend:Fig. 2A, 2B|comment:"Unlabeled bicoid DNA (Bcd) caused more than 85% inhibition."|dataset:Cardiac - Interactions involving cardiac related proteins|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2014/10/13	2014/11/10	-	-	intact-crc:4D0AB241BB04D4B5	false	32p radiolabel:?-?	-	-	-	psi-mi:"MI:0833"(autoradiography)	psi-mi:"MI:0833"(autoradiography)
intact:EBI-9847176	uniprotkb:Q99697-3	-	intact:EBI-1175243|ensembl:ENSP00000347192|ensembl:ENSP00000484909	psi-mi:bcd_probe(display_short)|psi-mi:EBI-9847176(display_long)	psi-mi:q99697-3(display_long)|uniprotkb:ARP1A(isoform synonym)|uniprotkb:PITX2(gene name)|psi-mi:PITX2(display_short)|uniprotkb:ARP1(gene name synonym)|uniprotkb:RGS(gene name synonym)|uniprotkb:RIEG(gene name synonym)|uniprotkb:RIEG1(gene name synonym)|uniprotkb:ALL1-responsive protein ARP1(gene name synonym)|uniprotkb:Homeobox protein PITX2(gene name synonym)|uniprotkb:Paired-like homeodomain transcription factor 2(gene name synonym)|uniprotkb:RIEG bicoid-related homeobox transcription factor(gene name synonym)|uniprotkb:Solurshin(gene name synonym)	psi-mi:"MI:0413"(electrophoretic mobility shift assay)	Amendt et al. (1998)	pubmed:9685346|imex:IM-23271	taxid:32630(synthetic construct)|taxid:32630(synthetic construct)	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:1332"(bhf-ucl)	intact:EBI-9867774|imex:IM-23271-8	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0680"(single stranded deoxyribonucleic acid)	psi-mi:"MI:0326"(protein)	-	intact:EBI-1175211(pitx2_human)|ensembl:ENSG00000164093(gene)|ensembl:ENST00000355080(transcript)|ensembl:ENST00000616641(transcript)	-	-	isoform-comment:"Variant in position: 58:F->L (in ASGD4)."	figure legend:Fig. 2A, 2B|comment:Unlabeled Prd caused less than 20% inhibition.|dataset:Cardiac - Interactions involving cardiac related proteins|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2014/10/13	2014/11/10	-	rogid:lbwqpXFBKq9v3kQyno+c46b48Wo9606	intact-crc:053C6F7A8DB2973D	false	32p radiolabel:?-?	-	-	-	psi-mi:"MI:0833"(autoradiography)	psi-mi:"MI:0833"(autoradiography)
intact:EBI-9847176	intact:EBI-9847313	-	-	psi-mi:bcd_probe(display_short)|psi-mi:EBI-9847176(display_long)	psi-mi:prd(display_short)|psi-mi:EBI-9847313(display_long)	psi-mi:"MI:0413"(electrophoretic mobility shift assay)	Amendt et al. (1998)	pubmed:9685346|imex:IM-23271	taxid:32630(synthetic construct)|taxid:32630(synthetic construct)	taxid:32630(synthetic construct)|taxid:32630(synthetic construct)	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:1332"(bhf-ucl)	intact:EBI-9867774|imex:IM-23271-8	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0941"(competitor)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0680"(single stranded deoxyribonucleic acid)	psi-mi:"MI:0680"(single stranded deoxyribonucleic acid)	-	pubmed:9685346(primary-reference)	-	-	-	figure legend:Fig. 2A, 2B|comment:Unlabeled Prd caused less than 20% inhibition.|dataset:Cardiac - Interactions involving cardiac related proteins|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2014/10/13	2014/11/10	-	-	intact-crc:053C6F7A8DB2973D	false	32p radiolabel:?-?	-	-	-	psi-mi:"MI:0833"(autoradiography)	psi-mi:"MI:0833"(autoradiography)
intact:EBI-9847176	uniprotkb:Q99697-3	-	intact:EBI-1175243|ensembl:ENSP00000347192|ensembl:ENSP00000484909	psi-mi:bcd_probe(display_short)|psi-mi:EBI-9847176(display_long)	psi-mi:q99697-3(display_long)|uniprotkb:ARP1A(isoform synonym)|uniprotkb:PITX2(gene name)|psi-mi:PITX2(display_short)|uniprotkb:ARP1(gene name synonym)|uniprotkb:RGS(gene name synonym)|uniprotkb:RIEG(gene name synonym)|uniprotkb:RIEG1(gene name synonym)|uniprotkb:ALL1-responsive protein ARP1(gene name synonym)|uniprotkb:Homeobox protein PITX2(gene name synonym)|uniprotkb:Paired-like homeodomain transcription factor 2(gene name synonym)|uniprotkb:RIEG bicoid-related homeobox transcription factor(gene name synonym)|uniprotkb:Solurshin(gene name synonym)	psi-mi:"MI:0413"(electrophoretic mobility shift assay)	Amendt et al. (1998)	pubmed:9685346|imex:IM-23271	taxid:32630(synthetic construct)|taxid:32630(synthetic construct)	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:1332"(bhf-ucl)	intact:EBI-9847352|imex:IM-23271-5	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0680"(single stranded deoxyribonucleic acid)	psi-mi:"MI:0326"(protein)	-	intact:EBI-1175211(pitx2_human)|ensembl:ENSG00000164093(gene)|ensembl:ENST00000355080(transcript)|ensembl:ENST00000616641(transcript)	-	-	isoform-comment:"Variant in position: 58:F->L (in ASGD4)."	figure legend:Fig. 2A, 2B|comment:Unlabeled Pit-1 DNA binding element caused 34% inhibition.|dataset:Cardiac - Interactions involving cardiac related proteins|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2014/10/13	2014/11/10	-	rogid:lbwqpXFBKq9v3kQyno+c46b48Wo9606	intact-crc:1627E73AB9C0A803	false	32p radiolabel:?-?	-	-	-	psi-mi:"MI:0833"(autoradiography)	psi-mi:"MI:0833"(autoradiography)
intact:EBI-9847176	intact:EBI-9847291	-	-	psi-mi:bcd_probe(display_short)|psi-mi:EBI-9847176(display_long)	psi-mi:pit-1(display_short)|psi-mi:EBI-9847291(display_long)	psi-mi:"MI:0413"(electrophoretic mobility shift assay)	Amendt et al. (1998)	pubmed:9685346|imex:IM-23271	taxid:32630(synthetic construct)|taxid:32630(synthetic construct)	taxid:32630(synthetic construct)|taxid:32630(synthetic construct)	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:1332"(bhf-ucl)	intact:EBI-9847352|imex:IM-23271-5	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0941"(competitor)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0680"(single stranded deoxyribonucleic acid)	psi-mi:"MI:0680"(single stranded deoxyribonucleic acid)	-	pubmed:9685346(primary-reference)	-	-	-	figure legend:Fig. 2A, 2B|comment:Unlabeled Pit-1 DNA binding element caused 34% inhibition.|dataset:Cardiac - Interactions involving cardiac related proteins|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2014/10/13	2014/11/10	-	-	intact-crc:1627E73AB9C0A803	false	32p radiolabel:?-?	-	-	-	psi-mi:"MI:0833"(autoradiography)	psi-mi:"MI:0833"(autoradiography)
intact:EBI-9847176	uniprotkb:Q99697-3	-	intact:EBI-1175243|ensembl:ENSP00000347192|ensembl:ENSP00000484909	psi-mi:bcd_probe(display_short)|psi-mi:EBI-9847176(display_long)	psi-mi:q99697-3(display_long)|uniprotkb:ARP1A(isoform synonym)|uniprotkb:PITX2(gene name)|psi-mi:PITX2(display_short)|uniprotkb:ARP1(gene name synonym)|uniprotkb:RGS(gene name synonym)|uniprotkb:RIEG(gene name synonym)|uniprotkb:RIEG1(gene name synonym)|uniprotkb:ALL1-responsive protein ARP1(gene name synonym)|uniprotkb:Homeobox protein PITX2(gene name synonym)|uniprotkb:Paired-like homeodomain transcription factor 2(gene name synonym)|uniprotkb:RIEG bicoid-related homeobox transcription factor(gene name synonym)|uniprotkb:Solurshin(gene name synonym)	psi-mi:"MI:0413"(electrophoretic mobility shift assay)	Amendt et al. (1998)	pubmed:9685346|imex:IM-23271	taxid:32630(synthetic construct)|taxid:32630(synthetic construct)	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:1332"(bhf-ucl)	intact:EBI-9867861|imex:IM-23271-13	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0680"(single stranded deoxyribonucleic acid)	psi-mi:"MI:0326"(protein)	-	intact:EBI-1175211(pitx2_human)|ensembl:ENSG00000164093(gene)|ensembl:ENST00000355080(transcript)|ensembl:ENST00000616641(transcript)	-	-	isoform-comment:"Variant in position: 58:F->L (in ASGD4)."	figure legend:Fig. 3A, 3B|comment:"The mutant Pitx2 T68P protein showed an overall increase in binding to all of the competitor DNAs (except the unlabeled bicoid DNA). The ability of these nonbicoid DNA competitors to bind Pitx2 T68P demonstrates a slight loss of binding specificity by this mutant."|dataset:Cardiac - Interactions involving cardiac related proteins|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2014/10/13	2014/11/10	-	rogid:lbwqpXFBKq9v3kQyno+c46b48Wo9606	intact-crc:A0DC346A718E0E76	false	32p radiolabel:?-?	mutation:68-68	-	-	psi-mi:"MI:0833"(autoradiography)	psi-mi:"MI:0833"(autoradiography)
intact:EBI-9847176	intact:EBI-9847511	-	-	psi-mi:bcd_probe(display_short)|psi-mi:EBI-9847176(display_long)	psi-mi:nkx-2.1(display_short)|psi-mi:EBI-9847511(display_long)	psi-mi:"MI:0413"(electrophoretic mobility shift assay)	Amendt et al. (1998)	pubmed:9685346|imex:IM-23271	taxid:32630(synthetic construct)|taxid:32630(synthetic construct)	taxid:32630(synthetic construct)|taxid:32630(synthetic construct)	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:1332"(bhf-ucl)	intact:EBI-9867861|imex:IM-23271-13	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0941"(competitor)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0680"(single stranded deoxyribonucleic acid)	psi-mi:"MI:0680"(single stranded deoxyribonucleic acid)	-	pubmed:9685346(primary-reference)	-	-	-	figure legend:Fig. 3A, 3B|comment:"The mutant Pitx2 T68P protein showed an overall increase in binding to all of the competitor DNAs (except the unlabeled bicoid DNA). The ability of these nonbicoid DNA competitors to bind Pitx2 T68P demonstrates a slight loss of binding specificity by this mutant."|dataset:Cardiac - Interactions involving cardiac related proteins|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2014/10/13	2014/11/10	-	-	intact-crc:A0DC346A718E0E76	false	32p radiolabel:?-?	-	-	-	psi-mi:"MI:0833"(autoradiography)	psi-mi:"MI:0833"(autoradiography)
intact:EBI-9847176	uniprotkb:Q99697-3	-	intact:EBI-1175243|ensembl:ENSP00000347192|ensembl:ENSP00000484909	psi-mi:bcd_probe(display_short)|psi-mi:EBI-9847176(display_long)	psi-mi:q99697-3(display_long)|uniprotkb:ARP1A(isoform synonym)|uniprotkb:PITX2(gene name)|psi-mi:PITX2(display_short)|uniprotkb:ARP1(gene name synonym)|uniprotkb:RGS(gene name synonym)|uniprotkb:RIEG(gene name synonym)|uniprotkb:RIEG1(gene name synonym)|uniprotkb:ALL1-responsive protein ARP1(gene name synonym)|uniprotkb:Homeobox protein PITX2(gene name synonym)|uniprotkb:Paired-like homeodomain transcription factor 2(gene name synonym)|uniprotkb:RIEG bicoid-related homeobox transcription factor(gene name synonym)|uniprotkb:Solurshin(gene name synonym)	psi-mi:"MI:0413"(electrophoretic mobility shift assay)	Amendt et al. (1998)	pubmed:9685346|imex:IM-23271	taxid:32630(synthetic construct)|taxid:32630(synthetic construct)	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:1332"(bhf-ucl)	intact:EBI-9867838|imex:IM-23271-12	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0680"(single stranded deoxyribonucleic acid)	psi-mi:"MI:0326"(protein)	-	intact:EBI-1175211(pitx2_human)|ensembl:ENSG00000164093(gene)|ensembl:ENST00000355080(transcript)|ensembl:ENST00000616641(transcript)	-	-	isoform-comment:"Variant in position: 58:F->L (in ASGD4)."	figure legend:Fig. 3A, 3B|comment:"The mutant Pitx2 T68P protein showed an overall increase in binding to all of the competitor DNAs (except the unlabeled bicoid DNA). The ability of these nonbicoid DNA competitors to bind Pitx2 T68P demonstrates a slight loss of binding specificity by this mutant."|dataset:Cardiac - Interactions involving cardiac related proteins|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2014/10/13	2014/11/10	-	rogid:lbwqpXFBKq9v3kQyno+c46b48Wo9606	intact-crc:9FB00DD43BE8E05B	false	32p radiolabel:?-?	mutation:68-68	-	-	psi-mi:"MI:0833"(autoradiography)	psi-mi:"MI:0833"(autoradiography)
intact:EBI-9847176	intact:EBI-9847535	-	-	psi-mi:bcd_probe(display_short)|psi-mi:EBI-9847176(display_long)	psi-mi:ftz(display_short)|psi-mi:EBI-9847535(display_long)	psi-mi:"MI:0413"(electrophoretic mobility shift assay)	Amendt et al. (1998)	pubmed:9685346|imex:IM-23271	taxid:32630(synthetic construct)|taxid:32630(synthetic construct)	taxid:32630(synthetic construct)|taxid:32630(synthetic construct)	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:1332"(bhf-ucl)	intact:EBI-9867838|imex:IM-23271-12	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0941"(competitor)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0680"(single stranded deoxyribonucleic acid)	psi-mi:"MI:0680"(single stranded deoxyribonucleic acid)	-	pubmed:9685346(primary-reference)	-	-	-	figure legend:Fig. 3A, 3B|comment:"The mutant Pitx2 T68P protein showed an overall increase in binding to all of the competitor DNAs (except the unlabeled bicoid DNA). The ability of these nonbicoid DNA competitors to bind Pitx2 T68P demonstrates a slight loss of binding specificity by this mutant."|dataset:Cardiac - Interactions involving cardiac related proteins|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2014/10/13	2014/11/10	-	-	intact-crc:9FB00DD43BE8E05B	false	32p radiolabel:?-?	-	-	-	psi-mi:"MI:0833"(autoradiography)	psi-mi:"MI:0833"(autoradiography)
intact:EBI-9847176	uniprotkb:Q99697-3	-	intact:EBI-1175243|ensembl:ENSP00000347192|ensembl:ENSP00000484909	psi-mi:bcd_probe(display_short)|psi-mi:EBI-9847176(display_long)	psi-mi:q99697-3(display_long)|uniprotkb:ARP1A(isoform synonym)|uniprotkb:PITX2(gene name)|psi-mi:PITX2(display_short)|uniprotkb:ARP1(gene name synonym)|uniprotkb:RGS(gene name synonym)|uniprotkb:RIEG(gene name synonym)|uniprotkb:RIEG1(gene name synonym)|uniprotkb:ALL1-responsive protein ARP1(gene name synonym)|uniprotkb:Homeobox protein PITX2(gene name synonym)|uniprotkb:Paired-like homeodomain transcription factor 2(gene name synonym)|uniprotkb:RIEG bicoid-related homeobox transcription factor(gene name synonym)|uniprotkb:Solurshin(gene name synonym)	psi-mi:"MI:0413"(electrophoretic mobility shift assay)	Amendt et al. (1998)	pubmed:9685346|imex:IM-23271	taxid:32630(synthetic construct)|taxid:32630(synthetic construct)	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:1332"(bhf-ucl)	intact:EBI-9867822|imex:IM-23271-11	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0680"(single stranded deoxyribonucleic acid)	psi-mi:"MI:0326"(protein)	-	intact:EBI-1175211(pitx2_human)|ensembl:ENSG00000164093(gene)|ensembl:ENST00000355080(transcript)|ensembl:ENST00000616641(transcript)	-	-	isoform-comment:"Variant in position: 58:F->L (in ASGD4)."	figure legend:Fig. 3A, 3B|comment:"The mutant Pitx2 T68P protein showed an overall increase in binding to all of the competitor DNAs (except the unlabeled bicoid DNA). The ability of these nonbicoid DNA competitors to bind Pitx2 T68P demonstrates a slight loss of binding specificity by this mutant."|dataset:Cardiac - Interactions involving cardiac related proteins|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2014/10/13	2014/11/10	-	rogid:lbwqpXFBKq9v3kQyno+c46b48Wo9606	intact-crc:3C75764254167B84	false	32p radiolabel:?-?	mutation:68-68	-	-	psi-mi:"MI:0833"(autoradiography)	psi-mi:"MI:0833"(autoradiography)
intact:EBI-9847176	intact:EBI-9847291	-	-	psi-mi:bcd_probe(display_short)|psi-mi:EBI-9847176(display_long)	psi-mi:pit-1(display_short)|psi-mi:EBI-9847291(display_long)	psi-mi:"MI:0413"(electrophoretic mobility shift assay)	Amendt et al. (1998)	pubmed:9685346|imex:IM-23271	taxid:32630(synthetic construct)|taxid:32630(synthetic construct)	taxid:32630(synthetic construct)|taxid:32630(synthetic construct)	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:1332"(bhf-ucl)	intact:EBI-9867822|imex:IM-23271-11	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0941"(competitor)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0680"(single stranded deoxyribonucleic acid)	psi-mi:"MI:0680"(single stranded deoxyribonucleic acid)	-	pubmed:9685346(primary-reference)	-	-	-	figure legend:Fig. 3A, 3B|comment:"The mutant Pitx2 T68P protein showed an overall increase in binding to all of the competitor DNAs (except the unlabeled bicoid DNA). The ability of these nonbicoid DNA competitors to bind Pitx2 T68P demonstrates a slight loss of binding specificity by this mutant."|dataset:Cardiac - Interactions involving cardiac related proteins|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2014/10/13	2014/11/10	-	-	intact-crc:3C75764254167B84	false	32p radiolabel:?-?	-	-	-	psi-mi:"MI:0833"(autoradiography)	psi-mi:"MI:0833"(autoradiography)
intact:EBI-9847176	uniprotkb:Q99697-3	-	intact:EBI-1175243|ensembl:ENSP00000347192|ensembl:ENSP00000484909	psi-mi:bcd_probe(display_short)|psi-mi:EBI-9847176(display_long)	psi-mi:q99697-3(display_long)|uniprotkb:ARP1A(isoform synonym)|uniprotkb:PITX2(gene name)|psi-mi:PITX2(display_short)|uniprotkb:ARP1(gene name synonym)|uniprotkb:RGS(gene name synonym)|uniprotkb:RIEG(gene name synonym)|uniprotkb:RIEG1(gene name synonym)|uniprotkb:ALL1-responsive protein ARP1(gene name synonym)|uniprotkb:Homeobox protein PITX2(gene name synonym)|uniprotkb:Paired-like homeodomain transcription factor 2(gene name synonym)|uniprotkb:RIEG bicoid-related homeobox transcription factor(gene name synonym)|uniprotkb:Solurshin(gene name synonym)	psi-mi:"MI:0413"(electrophoretic mobility shift assay)	Amendt et al. (1998)	pubmed:9685346|imex:IM-23271	taxid:32630(synthetic construct)|taxid:32630(synthetic construct)	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:1332"(bhf-ucl)	intact:EBI-9847486|imex:IM-23271-6	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0680"(single stranded deoxyribonucleic acid)	psi-mi:"MI:0326"(protein)	-	intact:EBI-1175211(pitx2_human)|ensembl:ENSG00000164093(gene)|ensembl:ENST00000355080(transcript)|ensembl:ENST00000616641(transcript)	-	-	isoform-comment:"Variant in position: 58:F->L (in ASGD4)."	figure legend:Fig. 3A, 3B|comment:"The mutant Pitx2 T68P protein showed an overall increase in binding to all of the competitor DNAs (except the unlabeled bicoid DNA). The ability of these nonbicoid DNA competitors to bind Pitx2 T68P demonstrates a slight loss of binding specificity by this mutant."|dataset:Cardiac - Interactions involving cardiac related proteins|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2014/10/13	2014/11/10	-	rogid:lbwqpXFBKq9v3kQyno+c46b48Wo9606	intact-crc:F16C4BC6D78224F7	false	32p radiolabel:?-?	mutation:68-68	-	-	psi-mi:"MI:0833"(autoradiography)	psi-mi:"MI:0833"(autoradiography)
intact:EBI-9847176	intact:EBI-9847527	-	-	psi-mi:bcd_probe(display_short)|psi-mi:EBI-9847176(display_long)	psi-mi:mu 9(display_short)|psi-mi:EBI-9847527(display_long)	psi-mi:"MI:0413"(electrophoretic mobility shift assay)	Amendt et al. (1998)	pubmed:9685346|imex:IM-23271	taxid:32630(synthetic construct)|taxid:32630(synthetic construct)	taxid:32630(synthetic construct)|taxid:32630(synthetic construct)	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:1332"(bhf-ucl)	intact:EBI-9847486|imex:IM-23271-6	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0941"(competitor)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0680"(single stranded deoxyribonucleic acid)	psi-mi:"MI:0680"(single stranded deoxyribonucleic acid)	-	pubmed:9685346(primary-reference)	-	-	-	figure legend:Fig. 3A, 3B|comment:"The mutant Pitx2 T68P protein showed an overall increase in binding to all of the competitor DNAs (except the unlabeled bicoid DNA). The ability of these nonbicoid DNA competitors to bind Pitx2 T68P demonstrates a slight loss of binding specificity by this mutant."|dataset:Cardiac - Interactions involving cardiac related proteins|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2014/10/13	2014/11/10	-	-	intact-crc:F16C4BC6D78224F7	false	32p radiolabel:?-?	-	-	-	psi-mi:"MI:0833"(autoradiography)	psi-mi:"MI:0833"(autoradiography)
intact:EBI-9847176	uniprotkb:Q99697-3	-	intact:EBI-1175243|ensembl:ENSP00000347192|ensembl:ENSP00000484909	psi-mi:bcd_probe(display_short)|psi-mi:EBI-9847176(display_long)	psi-mi:q99697-3(display_long)|uniprotkb:ARP1A(isoform synonym)|uniprotkb:PITX2(gene name)|psi-mi:PITX2(display_short)|uniprotkb:ARP1(gene name synonym)|uniprotkb:RGS(gene name synonym)|uniprotkb:RIEG(gene name synonym)|uniprotkb:RIEG1(gene name synonym)|uniprotkb:ALL1-responsive protein ARP1(gene name synonym)|uniprotkb:Homeobox protein PITX2(gene name synonym)|uniprotkb:Paired-like homeodomain transcription factor 2(gene name synonym)|uniprotkb:RIEG bicoid-related homeobox transcription factor(gene name synonym)|uniprotkb:Solurshin(gene name synonym)	psi-mi:"MI:0413"(electrophoretic mobility shift assay)	Amendt et al. (1998)	pubmed:9685346|imex:IM-23271	taxid:32630(synthetic construct)|taxid:32630(synthetic construct)	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:1332"(bhf-ucl)	intact:EBI-9867802|imex:IM-23271-10	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0680"(single stranded deoxyribonucleic acid)	psi-mi:"MI:0326"(protein)	-	intact:EBI-1175211(pitx2_human)|ensembl:ENSG00000164093(gene)|ensembl:ENST00000355080(transcript)|ensembl:ENST00000616641(transcript)	-	-	isoform-comment:"Variant in position: 58:F->L (in ASGD4)."	figure legend:Fig. 3A, 3B|comment:"The mutant Pitx2 T68P protein showed an overall increase in binding to all of the competitor DNAs (except the unlabeled bicoid DNA). The ability of these nonbicoid DNA competitors to bind Pitx2 T68P demonstrates a slight loss of binding specificity by this mutant."|dataset:Cardiac - Interactions involving cardiac related proteins|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2014/10/13	2014/11/10	-	rogid:lbwqpXFBKq9v3kQyno+c46b48Wo9606	intact-crc:A2FA07A6048E795F	false	32p radiolabel:?-?	mutation:68-68	-	-	psi-mi:"MI:0833"(autoradiography)	psi-mi:"MI:0833"(autoradiography)
intact:EBI-9847176	intact:EBI-9847313	-	-	psi-mi:bcd_probe(display_short)|psi-mi:EBI-9847176(display_long)	psi-mi:prd(display_short)|psi-mi:EBI-9847313(display_long)	psi-mi:"MI:0413"(electrophoretic mobility shift assay)	Amendt et al. (1998)	pubmed:9685346|imex:IM-23271	taxid:32630(synthetic construct)|taxid:32630(synthetic construct)	taxid:32630(synthetic construct)|taxid:32630(synthetic construct)	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:1332"(bhf-ucl)	intact:EBI-9867802|imex:IM-23271-10	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0941"(competitor)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0680"(single stranded deoxyribonucleic acid)	psi-mi:"MI:0680"(single stranded deoxyribonucleic acid)	-	pubmed:9685346(primary-reference)	-	-	-	figure legend:Fig. 3A, 3B|comment:"The mutant Pitx2 T68P protein showed an overall increase in binding to all of the competitor DNAs (except the unlabeled bicoid DNA). The ability of these nonbicoid DNA competitors to bind Pitx2 T68P demonstrates a slight loss of binding specificity by this mutant."|dataset:Cardiac - Interactions involving cardiac related proteins|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2014/10/13	2014/11/10	-	-	intact-crc:A2FA07A6048E795F	false	32p radiolabel:?-?	-	-	-	psi-mi:"MI:0833"(autoradiography)	psi-mi:"MI:0833"(autoradiography)
uniprotkb:Q99697-3	intact:EBI-9847176	intact:EBI-1175243|ensembl:ENSP00000347192|ensembl:ENSP00000484909	-	psi-mi:q99697-3(display_long)|uniprotkb:ARP1A(isoform synonym)|uniprotkb:PITX2(gene name)|psi-mi:PITX2(display_short)|uniprotkb:ARP1(gene name synonym)|uniprotkb:RGS(gene name synonym)|uniprotkb:RIEG(gene name synonym)|uniprotkb:RIEG1(gene name synonym)|uniprotkb:ALL1-responsive protein ARP1(gene name synonym)|uniprotkb:Homeobox protein PITX2(gene name synonym)|uniprotkb:Paired-like homeodomain transcription factor 2(gene name synonym)|uniprotkb:RIEG bicoid-related homeobox transcription factor(gene name synonym)|uniprotkb:Solurshin(gene name synonym)	psi-mi:bcd_probe(display_short)|psi-mi:EBI-9847176(display_long)	psi-mi:"MI:0413"(electrophoretic mobility shift assay)	Amendt et al. (1998)	pubmed:9685346|imex:IM-23271	taxid:9606(human)|taxid:9606(Homo sapiens)	taxid:32630(synthetic construct)|taxid:32630(synthetic construct)	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:1332"(bhf-ucl)	intact:EBI-9867883|imex:IM-23271-14	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0680"(single stranded deoxyribonucleic acid)	intact:EBI-1175211(pitx2_human)|ensembl:ENSG00000164093(gene)|ensembl:ENST00000355080(transcript)|ensembl:ENST00000616641(transcript)	-	-	isoform-comment:"Variant in position: 58:F->L (in ASGD4)."	-	figure legend:Fig. 1C, 3A, 3C, 6|agonist:"Pit-1 (P10037)"|comment:"EMSAs demonstrated binding of Pitx2 and Pitx2 T68P mutant to the bicoid element (Fig. 1C).   Binding to Pitx2 L54Q  mutant was not detected most likely because of protein instability."|dataset:Cardiac - Interactions involving cardiac related proteins|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	kd:58.0x10^-9(molar)	2014/10/13	2014/11/10	rogid:lbwqpXFBKq9v3kQyno+c46b48Wo9606	-	intact-crc:709D28BF43D18262	false	mutation:68-68	32p radiolabel:?-?	-	-	psi-mi:"MI:0833"(autoradiography)	psi-mi:"MI:0833"(autoradiography)
uniprotkb:Q99697-3	uniprotkb:P10037	intact:EBI-1175243|ensembl:ENSP00000347192|ensembl:ENSP00000484909	intact:EBI-9825525|uniprotkb:Q63431|ensembl:ENSRNOP00000000905	psi-mi:q99697-3(display_long)|uniprotkb:ARP1A(isoform synonym)|uniprotkb:PITX2(gene name)|psi-mi:PITX2(display_short)|uniprotkb:ARP1(gene name synonym)|uniprotkb:RGS(gene name synonym)|uniprotkb:RIEG(gene name synonym)|uniprotkb:RIEG1(gene name synonym)|uniprotkb:ALL1-responsive protein ARP1(gene name synonym)|uniprotkb:Homeobox protein PITX2(gene name synonym)|uniprotkb:Paired-like homeodomain transcription factor 2(gene name synonym)|uniprotkb:RIEG bicoid-related homeobox transcription factor(gene name synonym)|uniprotkb:Solurshin(gene name synonym)	psi-mi:pit1_rat(display_long)|uniprotkb:Pou1f1(gene name)|psi-mi:Pou1f1(display_short)|uniprotkb:Ghf-1(gene name synonym)|uniprotkb:Pit-1(gene name synonym)|uniprotkb:Pit1(gene name synonym)|uniprotkb:Growth hormone factor 1(gene name synonym)	psi-mi:"MI:0096"(pull down)	Amendt et al. (1998)	pubmed:9685346|imex:IM-23271	taxid:9606(human)|taxid:9606(Homo sapiens)	taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)")	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:1332"(bhf-ucl)	intact:EBI-9847553|imex:IM-23271-1	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	intact:EBI-1175211(pitx2_human)|ensembl:ENSG00000164093(gene)|ensembl:ENST00000355080(transcript)|ensembl:ENST00000616641(transcript)	rgd:3367|refseq:XP_006248036.1|ensembl:ENSRNOG00000000715(gene)|ensembl:ENSRNOT00000000905(transcript)|go:"GO:0000122"(negative regulation of transcription by RNA polymerase II)|go:"GO:0000978"(RNA polymerase II cis-regulatory region sequence-specific DNA binding)|go:"GO:0000981"(DNA-binding transcription factor activity, RNA polymerase II-specific)|go:"GO:0001228"(DNA-binding transcription activator activity, RNA polymerase II-specific)|go:"GO:0001708"(cell fate specification)|go:"GO:0003677"(DNA binding)|go:"GO:0003682"(chromatin binding)|go:"GO:0003700"(DNA-binding transcription factor activity)|go:"GO:0005634"(nucleus)|go:"GO:0005654"(nucleoplasm)|go:"GO:0005829"(cytosol)|go:"GO:0006355"(regulation of transcription, DNA-templated)|go:"GO:0006357"(regulation of transcription by RNA polymerase II)|go:"GO:0008284"(positive regulation of cell population proliferation)|go:"GO:0008340"(determination of adult lifespan)|go:"GO:0021983"(pituitary gland development)|go:"GO:0021984"(adenohypophysis development)|go:"GO:0030183"(B cell differentiation)|go:"GO:0032962"(positive regulation of inositol trisphosphate biosynthetic process)|go:"GO:0040018"(positive regulation of multicellular organism growth)|go:"GO:0043565"(sequence-specific DNA binding)|go:"GO:0043567"(regulation of insulin-like growth factor receptor signaling pathway)|go:"GO:0045893"(positive regulation of transcription, DNA-templated)|go:"GO:0045944"(positive regulation of transcription by RNA polymerase II)|go:"GO:0060126"(somatotropin secreting cell differentiation)|go:"GO:0060133"(somatotropin secreting cell development)|go:"GO:0061629"(RNA polymerase II-specific DNA-binding transcription factor binding)|go:"GO:1990837"(sequence-specific double-stranded DNA binding)|interpro:IPR000327(POU-specific)|interpro:IPR001356(Homeobox)|interpro:IPR009057(Homeodomain-like)|interpro:IPR010982(Lambda repressor-like, DNA-binding)|interpro:IPR013847(POU)|interpro:IPR015586(Pituitary-specific positive transcription factor 1)|interpro:IPR017970|rcsb pdb:1AU7	-	isoform-comment:"Variant in position: 58:F->L (in ASGD4)."	-	figure legend:Fig. 7|causality statement:O93385-2 and P10037 increase expression at RNA level of  ENSRNOG00000017374.|dataset:Cardiac - Interactions involving cardiac related proteins|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2014/10/13	2014/11/10	rogid:lbwqpXFBKq9v3kQyno+c46b48Wo9606	rogid:qGfEZ60egM4JNpLKiVLMXKgLphU10116	intact-crc:2A243333310725F0|rigid:g7AFreyB5pfKKOp5gizIabvxXzI	false	glutathione s tranferase tag:n-n	-	-	-	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)