#ID(s) interactor A	ID(s) interactor B	Alt. ID(s) interactor A	Alt. ID(s) interactor B	Alias(es) interactor A	Alias(es) interactor B	Interaction detection method(s)	Publication 1st author(s)	Publication Identifier(s)	Taxid interactor A	Taxid interactor B	Interaction type(s)	Source database(s)	Interaction identifier(s)	Confidence value(s)	Expansion method(s)	Biological role(s) interactor A	Biological role(s) interactor B	Experimental role(s) interactor A	Experimental role(s) interactor B	Type(s) interactor A	Type(s) interactor B	Xref(s) interactor A	Xref(s) interactor B	Interaction Xref(s)	Annotation(s) interactor A	Annotation(s) interactor B	Interaction annotation(s)	Host organism(s)	Interaction parameter(s)	Creation date	Update date	Checksum(s) interactor A	Checksum(s) interactor B	Interaction Checksum(s)	Negative	Feature(s) interactor A	Feature(s) interactor B	Stoichiometry(s) interactor A	Stoichiometry(s) interactor B	Identification method participant A	Identification method participant B
uniprotkb:P29295	uniprotkb:Q12292	intact:EBI-8536|uniprotkb:D6W3G5|ensemblfungi:YPL204W	intact:EBI-36362|uniprotkb:D6W1Y5|ensemblfungi:YOL083W	psi-mi:hrr25_yeast(display_long)|uniprotkb:YPL204W(locus name)|uniprotkb:HRR25(gene name)|psi-mi:HRR25(display_short)	psi-mi:atg34_yeast(display_long)|uniprotkb:ATG34(gene name)|psi-mi:ATG34(display_short)|uniprotkb:O0957(orf name)|uniprotkb:YOL083W(locus name)|uniprotkb:ATG19-B(gene name synonym)	psi-mi:"MI:0424"(protein kinase assay)	Mochida et al. (2014)	pubmed:25281559|imex:IM-23187	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	psi-mi:"MI:0217"(phosphorylation reaction)	psi-mi:"MI:0471"(MINT)	intact:EBI-9823956|imex:IM-23187-1	-	-	psi-mi:"MI:0501"(enzyme)	psi-mi:"MI:0502"(enzyme target)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_015120.1|sgd:S000006125|mint:P29295|dip:DIP-157N|ensemblfungi:YPL204W(gene)|ensemblfungi:YPL204W_mRNA(transcript)|go:"GO:0000407"(phagophore assembly site)|go:"GO:0000775"(chromosome, centromeric region)|go:"GO:0002098"(tRNA wobble uridine modification)|go:"GO:0004672"(protein kinase activity)|go:"GO:0004674"(protein serine/threonine kinase activity)|go:"GO:0004713"(protein tyrosine kinase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005634"(nucleus)|go:"GO:0005654"(nucleoplasm)|go:"GO:0005730"(nucleolus)|go:"GO:0005737"(cytoplasm)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005816"(spindle pole body)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0005934"(cellular bud tip)|go:"GO:0005935"(cellular bud neck)|go:"GO:0006281"(DNA repair)|go:"GO:0006468"(protein phosphorylation)|go:"GO:0006897"(endocytosis)|go:"GO:0018105"(peptidyl-serine phosphorylation)|go:"GO:0018108"(peptidyl-tyrosine phosphorylation)|go:"GO:0030242"(autophagy of peroxisome)|go:"GO:0030688"(preribosome, small subunit precursor)|go:"GO:0032880"(regulation of protein localization)|go:"GO:0033551"(monopolin complex)|go:"GO:0038083"(peptidyl-tyrosine autophosphorylation)|go:"GO:0042273"(ribosomal large subunit biogenesis)|go:"GO:0042274"(ribosomal small subunit biogenesis)|go:"GO:0042802"(identical protein binding)|go:"GO:0051455"(monopolar spindle attachment to meiosis I kinetochore)|go:"GO:0060628"(regulation of ER to Golgi vesicle-mediated transport)|go:"GO:0106214"(regulation of vesicle fusion with Golgi apparatus)|go:"GO:0106310"(protein serine kinase activity)|go:"GO:0106311"|go:"GO:2000370"(positive regulation of clathrin-dependent endocytosis)|go:"GO:2000785"(regulation of autophagosome assembly)|go:"GO:2001159"(regulation of protein localization by the Cvt pathway)|interpro:IPR000719(Protein kinase, core)|interpro:IPR008271(Serine/threonine protein kinase, active site)|interpro:IPR011009(Protein kinase-like)|interpro:IPR017441(Protein kinase ATP binding, conserved site)|interpro:IPR030509|rcsb pdb:4XHL|rcsb pdb:5CYZ|rcsb pdb:5CZO|reactome:R-SCE-204005|reactome:R-SCE-6791226|reactome:R-SCE-9013405	refseq:NP_014558.1|sgd:S000005443|dip:DIP-1598N|ensemblfungi:YOL083W(gene)|ensemblfungi:YOL083W_mRNA(transcript)|go:"GO:0005829"(cytosol)|go:"GO:0030674"(protein-macromolecule adaptor activity)|go:"GO:0034045"(phagophore assembly site membrane)|go:"GO:0034270"(Cvt complex)|go:"GO:0034622"|go:"GO:0034629"|go:"GO:0071211"(protein targeting to vacuole involved in autophagy)|interpro:IPR024543|rcsb pdb:2KZK	-	crc64:C4D3107A470E8BAF	crc64:5F339CC6AC2824D5	figure legend:f1c|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2014/09/22	2014/10/16	rogid:tS44OZjZHYS5GJDZM43ljVtw9SA559292	rogid:Kc60pek1GGfNXsRDx1GTvwkgk6o559292	intact-crc:DE4C1FDE5FD367DE|rigid:MsAV+p4fXsrId1HtSt84zIYMSgw	false	mutation disrupting interaction:38-38	glutathione s tranferase tag:?-?	-	-	psi-mi:"MI:0705"(anti tag western blot)	psi-mi:"MI:0421"(identification by antibody)
uniprotkb:Q12292	uniprotkb:P22855	intact:EBI-36362|uniprotkb:D6W1Y5|ensemblfungi:YOL083W	intact:EBI-10398|ensemblfungi:YGL156W|uniprotkb:D6VTZ5	psi-mi:atg34_yeast(display_long)|uniprotkb:ATG34(gene name)|psi-mi:ATG34(display_short)|uniprotkb:O0957(orf name)|uniprotkb:YOL083W(locus name)|uniprotkb:ATG19-B(gene name synonym)	psi-mi:man1_yeast(display_long)|uniprotkb:AMS1(gene name)|psi-mi:AMS1(display_short)|uniprotkb:YGL156W(locus name)|uniprotkb:G1861(orf name)|uniprotkb:Alpha-D-mannoside mannohydrolase(gene name synonym)	psi-mi:"MI:0416"(fluorescence microscopy)	Mochida et al. (2014)	pubmed:25281559|imex:IM-23187	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	psi-mi:"MI:0403"(colocalization)	psi-mi:"MI:0471"(MINT)	intact:EBI-9823974|imex:IM-23187-2	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_014558.1|sgd:S000005443|dip:DIP-1598N|ensemblfungi:YOL083W(gene)|ensemblfungi:YOL083W_mRNA(transcript)|go:"GO:0005829"(cytosol)|go:"GO:0030674"(protein-macromolecule adaptor activity)|go:"GO:0034045"(phagophore assembly site membrane)|go:"GO:0034270"(Cvt complex)|go:"GO:0034622"|go:"GO:0034629"|go:"GO:0071211"(protein targeting to vacuole involved in autophagy)|interpro:IPR024543|rcsb pdb:2KZK	refseq:NP_011359.1|sgd:S000003124|rcsb pdb:5JM0|interpro:IPR015341(Glycoside hydrolase, family 38, central region)|interpro:IPR027291|interpro:IPR028995|interpro:IPR037094|interpro:IPR041147|ensemblfungi:YGL156W(gene)|ensemblfungi:YGL156W_mRNA(transcript)|go:"GO:0000329"(fungal-type vacuole membrane)|go:"GO:0004559"(alpha-mannosidase activity)|go:"GO:0009313"(oligosaccharide catabolic process)|go:"GO:0019309"(mannose catabolic process)|go:"GO:0030246"(carbohydrate binding)|go:"GO:0034270"(Cvt complex)|go:"GO:0046872"(metal ion binding)|interpro:IPR000602(Glycoside hydrolase, family 38, core)|interpro:IPR011013(Glycoside hydrolase-type carbohydrate-binding)|interpro:IPR011330(Glycoside hydrolase/deacetylase, beta/alpha-barrel)|interpro:IPR011682(Glycosyl hydrolases 38, C-terminal)|reactome:R-SCE-8853383|dip:DIP-5497N	-	crc64:5F339CC6AC2824D5	crc64:624C818040FF5127	figure legend:f3b|comment:"We next asked what function of Atg34 requires Hrr25. During the autophagic transport of Ams1,  Atg34 first interacts with Ams1 oligomers to form a higher order assembly called the Ams1  complex, which is observed as dots in fluorescence microscopy of yeast cells expressing  Ams1–GFP [6,9]. Hrr25 knockdown affected neither formation of Ams1–GFP dots (Fig. 3A)  nor the colocalization of Ams1–mCherry and Atg34–GFP (Fig. 3B)."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	-	2014/09/22	2014/10/16	rogid:Kc60pek1GGfNXsRDx1GTvwkgk6o559292	rogid:OO5jp/V3itlWjql42JFHWTe1jfY559292	intact-crc:B472E99321E53365|rigid:mlMXpjMDd3egGlzT/PwfRbaHMm8	false	green fluorescent protein tag:?-?	mcherry fluorescent protein tag:?-?	-	-	psi-mi:"MI:0867"(tag visualisation by fluorescence)	psi-mi:"MI:0867"(tag visualisation by fluorescence)
uniprotkb:P22855	uniprotkb:Q12292	intact:EBI-10398|ensemblfungi:YGL156W|uniprotkb:D6VTZ5	intact:EBI-36362|uniprotkb:D6W1Y5|ensemblfungi:YOL083W	psi-mi:man1_yeast(display_long)|uniprotkb:AMS1(gene name)|psi-mi:AMS1(display_short)|uniprotkb:YGL156W(locus name)|uniprotkb:G1861(orf name)|uniprotkb:Alpha-D-mannoside mannohydrolase(gene name synonym)	psi-mi:atg34_yeast(display_long)|uniprotkb:ATG34(gene name)|psi-mi:ATG34(display_short)|uniprotkb:O0957(orf name)|uniprotkb:YOL083W(locus name)|uniprotkb:ATG19-B(gene name synonym)	psi-mi:"MI:0007"(anti tag coimmunoprecipitation)	Mochida et al. (2014)	pubmed:25281559|imex:IM-23187	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0471"(MINT)	intact:EBI-9824005|imex:IM-23187-3	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_011359.1|sgd:S000003124|rcsb pdb:5JM0|interpro:IPR015341(Glycoside hydrolase, family 38, central region)|interpro:IPR027291|interpro:IPR028995|interpro:IPR037094|interpro:IPR041147|ensemblfungi:YGL156W(gene)|ensemblfungi:YGL156W_mRNA(transcript)|go:"GO:0000329"(fungal-type vacuole membrane)|go:"GO:0004559"(alpha-mannosidase activity)|go:"GO:0009313"(oligosaccharide catabolic process)|go:"GO:0019309"(mannose catabolic process)|go:"GO:0030246"(carbohydrate binding)|go:"GO:0034270"(Cvt complex)|go:"GO:0046872"(metal ion binding)|interpro:IPR000602(Glycoside hydrolase, family 38, core)|interpro:IPR011013(Glycoside hydrolase-type carbohydrate-binding)|interpro:IPR011330(Glycoside hydrolase/deacetylase, beta/alpha-barrel)|interpro:IPR011682(Glycosyl hydrolases 38, C-terminal)|reactome:R-SCE-8853383|dip:DIP-5497N	refseq:NP_014558.1|sgd:S000005443|dip:DIP-1598N|ensemblfungi:YOL083W(gene)|ensemblfungi:YOL083W_mRNA(transcript)|go:"GO:0005829"(cytosol)|go:"GO:0030674"(protein-macromolecule adaptor activity)|go:"GO:0034045"(phagophore assembly site membrane)|go:"GO:0034270"(Cvt complex)|go:"GO:0034622"|go:"GO:0034629"|go:"GO:0071211"(protein targeting to vacuole involved in autophagy)|interpro:IPR024543|rcsb pdb:2KZK	-	crc64:624C818040FF5127	crc64:5F339CC6AC2824D5	figure legend:f3c|comment:"We next asked what function of Atg34 requires Hrr25. During the autophagic transport of Ams1,  Atg34 first interacts with Ams1 oligomers to form a higher order assembly called the Ams1  complex, which is observed as dots in fluorescence microscopy of yeast cells expressing  Ams1–GFP [6,9]. Hrr25 knockdown affected neither formation of Ams1–GFP dots (Fig. 3A)  nor the colocalization of Ams1–mCherry and Atg34–GFP (Fig. 3B)."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	-	2014/09/22	2014/10/16	rogid:OO5jp/V3itlWjql42JFHWTe1jfY559292	rogid:Kc60pek1GGfNXsRDx1GTvwkgk6o559292	intact-crc:D8BC47EF688830E1|rigid:mlMXpjMDd3egGlzT/PwfRbaHMm8	false	-	green fluorescent protein tag:?-?	-	-	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0705"(anti tag western blot)
uniprotkb:Q12292	uniprotkb:Q12527	intact:EBI-36362|uniprotkb:D6W1Y5|ensemblfungi:YOL083W	intact:EBI-31977|uniprotkb:D6W457|ensemblfungi:YPR049C	psi-mi:atg34_yeast(display_long)|uniprotkb:ATG34(gene name)|psi-mi:ATG34(display_short)|uniprotkb:O0957(orf name)|uniprotkb:YOL083W(locus name)|uniprotkb:ATG19-B(gene name synonym)	psi-mi:atg11_yeast(display_long)|uniprotkb:Cytoplasm to vacuole targeting protein 9(gene name synonym)|uniprotkb:ATG11(gene name)|psi-mi:ATG11(display_short)|uniprotkb:CVT9(gene name synonym)|uniprotkb:YPR049C(locus name)|uniprotkb:YP9499.07c(orf name)	psi-mi:"MI:0030"(cross-linking study)	Mochida et al. (2014)	pubmed:25281559|imex:IM-23187	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0471"(MINT)	intact:EBI-9824038|imex:IM-23187-4	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_014558.1|sgd:S000005443|dip:DIP-1598N|ensemblfungi:YOL083W(gene)|ensemblfungi:YOL083W_mRNA(transcript)|go:"GO:0005829"(cytosol)|go:"GO:0030674"(protein-macromolecule adaptor activity)|go:"GO:0034045"(phagophore assembly site membrane)|go:"GO:0034270"(Cvt complex)|go:"GO:0034622"|go:"GO:0034629"|go:"GO:0071211"(protein targeting to vacuole involved in autophagy)|interpro:IPR024543|rcsb pdb:2KZK	refseq:NP_015374.1|sgd:S000006253|dip:DIP-1492N|mint:Q12527|ensemblfungi:YPR049C(gene)|ensemblfungi:YPR049C_mRNA(transcript)|go:"GO:0000045"(autophagosome assembly)|go:"GO:0000149"(SNARE binding)|go:"GO:0000407"(phagophore assembly site)|go:"GO:0000422"(autophagy of mitochondrion)|go:"GO:0001934"(positive regulation of protein phosphorylation)|go:"GO:0005774"(vacuolar membrane)|go:"GO:0006914"(autophagy)|go:"GO:0019898"(extrinsic component of membrane)|go:"GO:0019901"(protein kinase binding)|go:"GO:0030242"(autophagy of peroxisome)|go:"GO:0032258"(cytoplasm to vacuole transport by the Cvt pathway)|go:"GO:0034045"(phagophore assembly site membrane)|go:"GO:0034497"(protein localization to phagophore assembly site)|go:"GO:0034629"|go:"GO:0034727"(piecemeal microautophagy of the nucleus)|go:"GO:0060090"(molecular adaptor activity)|go:"GO:0061709"(reticulophagy)|go:"GO:0140255"(regulation of cellular response to phosphate starvation)|go:"GO:1990316"(Atg1/ULK1 kinase complex)|go:"GO:2000786"(positive regulation of autophagosome assembly)|interpro:IPR019460|interpro:IPR040040|rcsb pdb:6VZF|reactome:R-SCE-1632852	-	crc64:5F339CC6AC2824D5	crc64:A904B92E9AE79449	figure legend:f4a|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:559292(yeast)|taxid:559292(Saccharomyces cerevisiae)	-	2014/09/22	2014/10/16	rogid:Kc60pek1GGfNXsRDx1GTvwkgk6o559292	rogid:26PElwvrl3YKwoQU7YdkmR0ln3o559292	intact-crc:02F905589283247B|rigid:x7ya7seqZC9iTjurLtQjek4luCg	false	-	green fluorescent protein tag:?-?	-	-	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0705"(anti tag western blot)