#ID(s) interactor A	ID(s) interactor B	Alt. ID(s) interactor A	Alt. ID(s) interactor B	Alias(es) interactor A	Alias(es) interactor B	Interaction detection method(s)	Publication 1st author(s)	Publication Identifier(s)	Taxid interactor A	Taxid interactor B	Interaction type(s)	Source database(s)	Interaction identifier(s)	Confidence value(s)	Expansion method(s)	Biological role(s) interactor A	Biological role(s) interactor B	Experimental role(s) interactor A	Experimental role(s) interactor B	Type(s) interactor A	Type(s) interactor B	Xref(s) interactor A	Xref(s) interactor B	Interaction Xref(s)	Annotation(s) interactor A	Annotation(s) interactor B	Interaction annotation(s)	Host organism(s)	Interaction parameter(s)	Creation date	Update date	Checksum(s) interactor A	Checksum(s) interactor B	Interaction Checksum(s)	Negative	Feature(s) interactor A	Feature(s) interactor B	Stoichiometry(s) interactor A	Stoichiometry(s) interactor B	Identification method participant A	Identification method participant B
uniprotkb:P02699	uniprotkb:P63000	intact:EBI-8592832|ensembl:ENSBTAP00000001730|intact:MINT-1507462	intact:EBI-413628|ensembl:ENSP00000258737|uniprotkb:O95501|uniprotkb:P15154|uniprotkb:Q3Y4D3|uniprotkb:Q5JAA8|uniprotkb:Q9BTB4	psi-mi:opsd_bovin(display_long)|uniprotkb:RHO(gene name)|psi-mi:RHO(display_short)	psi-mi:rac1_human(display_long)|uniprotkb:RAC1(gene name)|psi-mi:RAC1(display_short)|uniprotkb:MIG5(orf name)|uniprotkb:p21-Rac1(gene name synonym)|uniprotkb:Ras-like protein TC25(gene name synonym)|uniprotkb:Cell migration-inducing gene 5 protein(gene name synonym)|uniprotkb:TC25(gene name synonym)	psi-mi:"MI:0107"(surface plasmon resonance)	Köster et al. (2014)	pubmed:25243418|imex:IM-23185	taxid:9913(bovin)|taxid:9913("Bos taurus (Bovine)")	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0471"(MINT)	intact:EBI-9822518|imex:IM-23185-1	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	ensembl:ENSBTAG00000001310(gene)|ensembl:ENSBTAT00000001730(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0001750"(photoreceptor outer segment)|go:"GO:0001965"(G-protein alpha-subunit binding)|go:"GO:0002046"(opsin binding)|go:"GO:0005085"(guanyl-nucleotide exchange factor activity)|go:"GO:0005502"(11-cis retinal binding)|go:"GO:0005886"(plasma membrane)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0005911"(cell-cell junction)|go:"GO:0006468"(protein phosphorylation)|go:"GO:0007186"(G protein-coupled receptor signaling pathway)|go:"GO:0007601"(visual perception)|go:"GO:0007602"(phototransduction)|go:"GO:0007603"(phototransduction, visible light)|go:"GO:0008020"(G protein-coupled photoreceptor activity)|go:"GO:0008270"(zinc ion binding)|go:"GO:0009416"(response to light stimulus)|go:"GO:0016021"(integral component of membrane)|go:"GO:0016038"(absorption of visible light)|go:"GO:0016056"(rhodopsin mediated signaling pathway)|go:"GO:0018298"(protein-chromophore linkage)|go:"GO:0019867"(outer membrane)|go:"GO:0042622"(photoreceptor outer segment membrane)|go:"GO:0042802"(identical protein binding)|go:"GO:0043052"(thermotaxis)|go:"GO:0045494"(photoreceptor cell maintenance)|go:"GO:0050960"(detection of temperature stimulus involved in thermoception)|go:"GO:0060041"(retina development in camera-type eye)|go:"GO:0060342"(photoreceptor inner segment membrane)|go:"GO:0071482"(cellular response to light stimulus)|go:"GO:0097225"(sperm midpiece)|go:"GO:0097381"(photoreceptor disc membrane)|go:"GO:0097648"(G protein-coupled receptor complex)|go:"GO:1990763"(arrestin family protein binding)|go:"GO:1990913"(sperm head plasma membrane)|interpro:IPR000276(GPCR, rhodopsin-like)|interpro:IPR000732(Rhodopsin)|interpro:IPR001760(Opsin)|rcsb pdb:1NZS|rcsb pdb:1OV0|rcsb pdb:1OV1|rcsb pdb:1U19|interpro:IPR017452(GPCR, rhodopsin-like superfamily)|rcsb pdb:1VQX|rcsb pdb:2G87|rcsb pdb:2HPY|interpro:IPR019477|rcsb pdb:2I35|interpro:IPR027430|mint:P02699|rcsb pdb:1BOJ|rcsb pdb:1BOK|rcsb pdb:1EDS|rcsb pdb:1EDV|rcsb pdb:1EDW|rcsb pdb:2I36|rcsb pdb:2I37|rcsb pdb:2IQK|rcsb pdb:2J4Y|rcsb pdb:1EDX|rcsb pdb:2PED|rcsb pdb:2X72|rcsb pdb:3C9L|rcsb pdb:3C9M|rcsb pdb:3CAP|rcsb pdb:3DQB|rcsb pdb:3OAX|rcsb pdb:3PQR|rcsb pdb:3PXO|rcsb pdb:4A4M|rcsb pdb:4BEY|rcsb pdb:1F88|rcsb pdb:4BEZ|rcsb pdb:1FDF|rcsb pdb:1GZM|rcsb pdb:4J4Q|rcsb pdb:4PXF|rcsb pdb:4X1H|rcsb pdb:5DYS|rcsb pdb:5EN0|rcsb pdb:5TE3|rcsb pdb:5TE5|rcsb pdb:5WKT|rcsb pdb:6FK6|rcsb pdb:6FK7|rcsb pdb:6FK8|rcsb pdb:6FK9|rcsb pdb:6FKA|rcsb pdb:6FKB|rcsb pdb:6FKC|rcsb pdb:6FKD|rcsb pdb:6FUF|rcsb pdb:6NWE|rcsb pdb:6OFJ|rcsb pdb:6OY9|rcsb pdb:6OYA|rcsb pdb:6PEL|rcsb pdb:6PGS|rcsb pdb:6PH7|rcsb pdb:6QNO|reactome:R-BTA-2453902|reactome:R-BTA-2485179|reactome:R-BTA-2514859|reactome:R-BTA-418594|reactome:R-BTA-419771|reactome:R-BTA-5620916|rcsb pdb:1HZX|rcsb pdb:1JFP|rcsb pdb:1L9H|rcsb pdb:1LN6|rcsb pdb:1N3M|refseq:NP_001014890.1|dip:DIP-29225N	ensembl:ENSG00000136238(gene)|ensembl:ENST00000348035(transcript)|go:"GO:0000139"(Golgi membrane)|go:"GO:0001764"(neuron migration)|go:"GO:0001934"(positive regulation of protein phosphorylation)|go:"GO:0002551"(mast cell chemotaxis)|go:"GO:0003376"(sphingosine-1-phosphate receptor signaling pathway)|go:"GO:0003924"(GTPase activity)|go:"GO:0003925"(G protein activity)|go:"GO:0005525"(GTP binding)|go:"GO:0005737"(cytoplasm)|go:"GO:0005789"(endoplasmic reticulum membrane)|go:"GO:0005802"(trans-Golgi network)|go:"GO:0005829"(cytosol)|go:"GO:0005856"(cytoskeleton)|go:"GO:0005886"(plasma membrane)|go:"GO:0005925"(focal adhesion)|go:"GO:0005938"(cell cortex)|go:"GO:0006954"(inflammatory response)|go:"GO:0030334"(regulation of cell migration)|go:"GO:0030667"(secretory granule membrane)|go:"GO:0030865"(cortical cytoskeleton organization)|go:"GO:0031116"(positive regulation of microtubule polymerization)|go:"GO:0030041"(actin filament polymerization)|go:"GO:0031234"(extrinsic component of cytoplasmic side of plasma membrane)|go:"GO:0031267"(small GTPase binding)|go:"GO:0031410"(cytoplasmic vesicle)|go:"GO:0031529"(ruffle organization)|go:"GO:0031996"(thioesterase binding)|go:"GO:0032587"(ruffle membrane)|go:"GO:0032707"(negative regulation of interleukin-23 production)|go:"GO:0032956"(regulation of actin cytoskeleton organization)|go:"GO:0034446"(substrate adhesion-dependent cell spreading)|go:"GO:0035025"(positive regulation of Rho protein signal transduction)|go:"GO:0035556"(intracellular signal transduction)|go:"GO:0035774"(positive regulation of insulin secretion involved in cellular response to glucose stimulus)|go:"GO:0036464"(cytoplasmic ribonucleoprotein granule)|go:"GO:0007015"(actin filament organization)|go:"GO:0007155"(cell adhesion)|go:"GO:0007160"(cell-matrix adhesion)|go:"GO:0007163"(establishment or maintenance of cell polarity)|go:"GO:0007264"(small GTPase mediated signal transduction)|go:"GO:0008045"(motor neuron axon guidance)|go:"GO:0008283"(cell population proliferation)|go:"GO:0008360"(regulation of cell shape)|go:"GO:0008361"(regulation of cell size)|go:"GO:0009611"(response to wounding)|go:"GO:0009653"(anatomical structure morphogenesis)|go:"GO:0010310"(regulation of hydrogen peroxide metabolic process)|go:"GO:0010591"(regulation of lamellipodium assembly)|go:"GO:0010592"(positive regulation of lamellipodium assembly)|go:"GO:0010595"(positive regulation of endothelial cell migration)|go:"GO:0010764"(negative regulation of fibroblast migration)|go:"GO:0010811"(positive regulation of cell-substrate adhesion)|go:"GO:0016020"(membrane)|go:"GO:0016477"(cell migration)|go:"GO:0016601"(Rac protein signal transduction)|go:"GO:0019899"(enzyme binding)|go:"GO:0019901"(protein kinase binding)|go:"GO:0030027"(lamellipodium)|go:"GO:0030031"(cell projection assembly)|go:"GO:0030032"(lamellipodium assembly)|go:"GO:0030036"(actin cytoskeleton organization)|go:"GO:0042470"(melanosome)|reactome:R-HSA-2424491|refseq:NP_008839.2|refseq:NP_061485.1|go:"GO:0051496"(positive regulation of stress fiber assembly)|go:"GO:0051668"(localization within membrane)|go:"GO:0051894"(positive regulation of focal adhesion assembly)|go:"GO:0055038"(recycling endosome membrane)|go:"GO:0060071"(Wnt signaling pathway, planar cell polarity pathway)|go:"GO:0060263"(regulation of respiratory burst)|go:"GO:0070062"(extracellular exosome)|go:"GO:0071260"(cellular response to mechanical stimulus)|go:"GO:0071526"(semaphorin-plexin signaling pathway)|go:"GO:0090023"(positive regulation of neutrophil chemotaxis)|go:"GO:0097178"(ruffle assembly)|go:"GO:0098794"(postsynapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0101003"(ficolin-1-rich granule membrane)|go:"GO:1900026"(positive regulation of substrate adhesion-dependent cell spreading)|go:"GO:1902622"(regulation of neutrophil migration)|interpro:IPR001806(Ras GTPase)|interpro:IPR003578(Ras small GTPase, Rho type)|interpro:IPR005225(Small GTP-binding protein)|interpro:IPR027417|mint:P63000|rcsb pdb:1E96|rcsb pdb:1FOE|rcsb pdb:1G4U|rcsb pdb:1HE1|rcsb pdb:1HH4|rcsb pdb:1I4D|rcsb pdb:1I4L|rcsb pdb:1I4T|rcsb pdb:1MH1|rcsb pdb:1RYF|rcsb pdb:1RYH|rcsb pdb:2FJU|rcsb pdb:2H7V|rcsb pdb:2NZ8|rcsb pdb:2P2L|rcsb pdb:2RMK|rcsb pdb:2VRW|rcsb pdb:2WKP|rcsb pdb:2WKQ|rcsb pdb:2WKR|rcsb pdb:2YIN|rcsb pdb:3B13|rcsb pdb:3BJI|rcsb pdb:3RYT|rcsb pdb:3SBD|rcsb pdb:3SBE|rcsb pdb:3SU8|rcsb pdb:3SUA|rcsb pdb:3TH5|rcsb pdb:4GZL|rcsb pdb:4GZM|rcsb pdb:4YON|rcsb pdb:5FI0|rcsb pdb:5HZH|rcsb pdb:5N6O|rcsb pdb:5O33|rcsb pdb:5QQD|rcsb pdb:5QQE|rcsb pdb:5QQF|rcsb pdb:5QQG|rcsb pdb:5QQH|rcsb pdb:5QQI|rcsb pdb:5QQJ|rcsb pdb:5QQK|rcsb pdb:5QQL|rcsb pdb:5QQM|rcsb pdb:5QQN|rcsb pdb:5QU9|rcsb pdb:6AGP|rcsb pdb:6BC1|rcsb pdb:6TGC|rcsb pdb:6X1G|rcsb pdb:7AJK|reactome:R-HSA-114604|reactome:R-HSA-1257604|reactome:R-HSA-1433557|reactome:R-HSA-1445148|reactome:R-HSA-164944|reactome:R-HSA-193648|reactome:R-HSA-2029482|reactome:R-HSA-2219530|go:"GO:0042826"(histone deacetylase binding)|go:"GO:0042995"(cell projection)|go:"GO:0043197"(dendritic spine)|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0043652"(engulfment of apoptotic cell)|go:"GO:0044877"(protein-containing complex binding)|go:"GO:0045428"(regulation of nitric oxide biosynthetic process)|go:"GO:0045453"(bone resorption)|go:"GO:0045740"(positive regulation of DNA replication)|go:"GO:0048012"(hepatocyte growth factor receptor signaling pathway)|go:"GO:0048261"(negative regulation of receptor-mediated endocytosis)|go:"GO:0048870"(cell motility)|go:"GO:0051022"(Rho GDP-dissociation inhibitor binding)|go:"GO:0051117"(ATPase binding)|go:"GO:0051492"(regulation of stress fiber assembly)|reactome:R-HSA-3928662|reactome:R-HSA-3928664|reactome:R-HSA-3928665|reactome:R-HSA-389359|reactome:R-HSA-399954|reactome:R-HSA-399955|reactome:R-HSA-4086400|reactome:R-HSA-416550|reactome:R-HSA-418885|reactome:R-HSA-428540|reactome:R-HSA-428543|reactome:R-HSA-4420097|reactome:R-HSA-445144|reactome:R-HSA-5218920|reactome:R-HSA-5625740|reactome:R-HSA-5625900|reactome:R-HSA-5625970|reactome:R-HSA-5626467|reactome:R-HSA-5627123|reactome:R-HSA-5663213|reactome:R-HSA-5663220|reactome:R-HSA-5668599|reactome:R-HSA-5687128|reactome:R-HSA-6798695|reactome:R-HSA-6811558|reactome:R-HSA-8849471|reactome:R-HSA-8875555|reactome:R-HSA-9013149|reactome:R-HSA-9032759|reactome:R-HSA-9032845|reactome:R-HSA-9619229|reactome:R-HSA-9664422|reactome:R-HSA-9673324|reactome:R-HSA-983231|reactome:R-HSA-2871796|reactome:R-HSA-376172|dip:DIP-29260N	-	comment:mint|function:Photoreceptor required for image-forming vision at low light intensity. Required for photoreceptor cell viability after birth. Light-induced isomerization of 11-cis to all-trans retinal triggers a conformational change leading to G-protein activation and release of all-trans retinal	-	figure legend:f3|comment:"Rac1 bound to immobilized rhodopsin in a  concentration dependent manner (Figure 3A)."|comment:"The binding process was not triggered by light, since Rac1 bound to dark-adapted rhodopsin  in a similar manner as to light-activated rhodopsin and binding of Rac1 to rhodopsin was not  reversed by adding GTPγS (Figure 3B), which can be used to rapidly dissociate Gt from  rhodopsin that is immobilized on a sensor chip surface"|comment:Further, the amount of Rac1 interacting with rhodopsin was not increased by illumination as  can be seen in Figure 3C, where rhodopsin was bleached after Rac1 was injected into the flow  cell and the sensorgram was compared with a recording done in darkness|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2014/09/22	2014/10/16	rogid:ntvPNbJU0fcn+gajwUZzvfd/YCI9913	rogid:8rkYyZSFL5tIfbRNKZgP/UDxD+A9606	intact-crc:74FDFE3E9912E183|rigid:JE4TX3gaN3r/vC9rho0ErKAoBW4	false	-	-	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)