#ID(s) interactor A	ID(s) interactor B	Alt. ID(s) interactor A	Alt. ID(s) interactor B	Alias(es) interactor A	Alias(es) interactor B	Interaction detection method(s)	Publication 1st author(s)	Publication Identifier(s)	Taxid interactor A	Taxid interactor B	Interaction type(s)	Source database(s)	Interaction identifier(s)	Confidence value(s)	Expansion method(s)	Biological role(s) interactor A	Biological role(s) interactor B	Experimental role(s) interactor A	Experimental role(s) interactor B	Type(s) interactor A	Type(s) interactor B	Xref(s) interactor A	Xref(s) interactor B	Interaction Xref(s)	Annotation(s) interactor A	Annotation(s) interactor B	Interaction annotation(s)	Host organism(s)	Interaction parameter(s)	Creation date	Update date	Checksum(s) interactor A	Checksum(s) interactor B	Interaction Checksum(s)	Negative	Feature(s) interactor A	Feature(s) interactor B	Stoichiometry(s) interactor A	Stoichiometry(s) interactor B	Identification method participant A	Identification method participant B
uniprotkb:P51579	uniprotkb:P51577	intact:EBI-9511515|uniprotkb:R9PXR5|ensembl:ENSRNOP00000002558	intact:EBI-9511617|ensembl:ENSRNOP00000001752	psi-mi:p2rx6_rat(display_long)|uniprotkb:P2rx6(gene name)|psi-mi:P2rx6(display_short)|uniprotkb:P2rxl1(gene name synonym)|uniprotkb:ATP receptor(gene name synonym)|uniprotkb:P2XM(gene name synonym)|uniprotkb:Purinergic receptor(gene name synonym)|uniprotkb:Purinergic receptor P2X-like 1(gene name synonym)	psi-mi:p2rx4_rat(display_long)|uniprotkb:P2rx4(gene name)|psi-mi:P2rx4(display_short)|uniprotkb:ATP receptor(gene name synonym)|uniprotkb:Purinergic receptor(gene name synonym)	psi-mi:"MI:0416"(fluorescence microscopy)	Antonio et al. (2014)	pubmed:24815693|imex:IM-22591	taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)")	taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)")	psi-mi:"MI:0403"(colocalization)	psi-mi:"MI:0471"(MINT)	intact:EBI-9511606|imex:IM-22591-2	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	rgd:3243|refseq:NP_036853.2|ensembl:ENSRNOG00000001873(gene)|ensembl:ENSRNOT00000002558(transcript)|go:"GO:0001614"(purinergic nucleotide receptor activity)|go:"GO:0004931"(extracellularly ATP-gated cation channel activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005639"(integral component of nuclear inner membrane)|go:"GO:0005737"(cytoplasm)|go:"GO:0014069"(postsynaptic density)|go:"GO:0016020"(membrane)|go:"GO:0030054"(cell junction)|go:"GO:0033198"(response to ATP)|go:"GO:0043025"(neuronal cell body)|go:"GO:0043197"(dendritic spine)|go:"GO:0043235"(receptor complex)|go:"GO:0044877"(protein-containing complex binding)|go:"GO:0098688"(parallel fiber to Purkinje cell synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099060"(integral component of postsynaptic specialization membrane)|interpro:IPR001429(P2X purinoreceptor)|interpro:IPR003049(P2X6 purinoceptor)|interpro:IPR027309|reactome:R-RNO-139853|reactome:R-RNO-418346	rgd:62073|refseq:NP_113782.1|dip:DIP-48411N|ensembl:ENSRNOG00000001300(gene)|ensembl:ENSRNOT00000001752(transcript)|go:"GO:0001614"(purinergic nucleotide receptor activity)|go:"GO:0002028"(regulation of sodium ion transport)|go:"GO:0002931"(response to ischemia)|go:"GO:0004931"(extracellularly ATP-gated cation channel activity)|go:"GO:0005102"(signaling receptor binding)|go:"GO:0005507"(copper ion binding)|go:"GO:0005524"(ATP binding)|go:"GO:0005639"(integral component of nuclear inner membrane)|go:"GO:0005886"(plasma membrane)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0006809"(nitric oxide biosynthetic process)|go:"GO:0006816"(calcium ion transport)|go:"GO:0007165"(signal transduction)|go:"GO:0008217"(regulation of blood pressure)|go:"GO:0008270"(zinc ion binding)|go:"GO:0010524"(positive regulation of calcium ion transport into cytosol)|go:"GO:0010614"(negative regulation of cardiac muscle hypertrophy)|go:"GO:0014069"(postsynaptic density)|go:"GO:0016020"(membrane)|go:"GO:0019228"(neuronal action potential)|go:"GO:0019233"(sensory perception of pain)|go:"GO:0030054"(cell junction)|go:"GO:0030424"(axon)|go:"GO:0030425"(dendrite)|go:"GO:0033198"(response to ATP)|go:"GO:0034220"(ion transmembrane transport)|go:"GO:0034405"(response to fluid shear stress)|go:"GO:0035590"(purinergic nucleotide receptor signaling pathway)|go:"GO:0042311"(vasodilation)|go:"GO:0042802"(identical protein binding)|go:"GO:0043025"(neuronal cell body)|go:"GO:0043195"(terminal bouton)|go:"GO:0043197"(dendritic spine)|go:"GO:0043536"(positive regulation of blood vessel endothelial cell migration)|go:"GO:0044297"(cell body)|go:"GO:0045177"(apical part of cell)|go:"GO:0045202"(synapse)|go:"GO:0045296"(cadherin binding)|go:"GO:0048266"(behavioral response to pain)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0048678"(response to axon injury)|go:"GO:0050850"(positive regulation of calcium-mediated signaling)|go:"GO:0050920"(regulation of chemotaxis)|go:"GO:0050975"(sensory perception of touch)|go:"GO:0051897"(positive regulation of protein kinase B signaling)|go:"GO:0051899"(membrane depolarization)|go:"GO:0055117"(regulation of cardiac muscle contraction)|go:"GO:0055119"(relaxation of cardiac muscle)|go:"GO:0060079"(excitatory postsynaptic potential)|go:"GO:0070588"(calcium ion transmembrane transport)|go:"GO:0071294"(cellular response to zinc ion)|go:"GO:0071318"(cellular response to ATP)|go:"GO:0097190"(apoptotic signaling pathway)|go:"GO:0098662"(inorganic cation transmembrane transport)|go:"GO:0099604"(ligand-gated calcium channel activity)|go:"GO:1904141"(positive regulation of microglial cell migration)|go:"GO:2001028"(positive regulation of endothelial cell chemotaxis)|interpro:IPR001429(P2X purinoreceptor)|interpro:IPR003047(P2X4 purinoceptor)|interpro:IPR027309|rcsb pdb:2BP5|rcsb pdb:2RUP|reactome:R-RNO-139853|reactome:R-RNO-418346	-	-	-	figure legend:f1|comment:"Cells expressing various  combinations of P2X receptor subunits (P2X2/P2X4, P2X2/P2X6, P2X4/P2X6 and  P2X2/P2X4/P2X6) were fixed, permeabilized and incubated with appropriate primary  antibodies, followed by fluorophore-conjugated secondary antibodies (Sigma). Cells were  imaged by confocal laser scanning microscopy. As shown in Fig. 1, the immunostaining and  transfection patterns coincided exactly, and the absence of a particular subunit led to a  negative staining signal. The majority of co-transfected cells expressed each of the  transfected subunits, and in all cases the proteins appeared to be present throughout the cell."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:9606(human-tsa201)|taxid:9606(Homo sapiens subclone of human embryonic kidney 293 cell line that expresses simian virus 40 T antigen)	-	2014/04/28	2018/02/26	rogid:cTu+Raxv9pYr4tIXWDZiTfNHMl410116	rogid:ogsHtvyxaAmmFUflijf6YfBuW7U10116	intact-crc:66942CD53144EA91|rigid:Jk4CuU9vY633IomwwhnsYEzn5/o	false	his tag:?-?	ha tag:?-?	-	-	psi-mi:"MI:0707"(anti tag immunostaining)	psi-mi:"MI:0707"(anti tag immunostaining)
uniprotkb:P49653	uniprotkb:P51577	intact:EBI-9511543|uniprotkb:O54868	intact:EBI-9511617|ensembl:ENSRNOP00000001752	psi-mi:p2rx2_rat(display_long)|uniprotkb:P2rx2(gene name)|psi-mi:P2rx2(display_short)|uniprotkb:ATP receptor(gene name synonym)|uniprotkb:Purinergic receptor(gene name synonym)	psi-mi:p2rx4_rat(display_long)|uniprotkb:P2rx4(gene name)|psi-mi:P2rx4(display_short)|uniprotkb:ATP receptor(gene name synonym)|uniprotkb:Purinergic receptor(gene name synonym)	psi-mi:"MI:0416"(fluorescence microscopy)	Antonio et al. (2014)	pubmed:24815693|imex:IM-22591	taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)")	taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)")	psi-mi:"MI:0403"(colocalization)	psi-mi:"MI:0471"(MINT)	intact:EBI-9511670|imex:IM-22591-4	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	reactome:R-RNO-139853|reactome:R-RNO-418346|rgd:620251|refseq:NP_446108.2|dip:DIP-48329N|go:"GO:0001614"(purinergic nucleotide receptor activity)|go:"GO:0001666"(response to hypoxia)|go:"GO:0002931"(response to ischemia)|go:"GO:0003029"(detection of hypoxic conditions in blood by carotid body chemoreceptor signaling)|go:"GO:0004931"(extracellularly ATP-gated cation channel activity)|go:"GO:0005507"(copper ion binding)|go:"GO:0005524"(ATP binding)|go:"GO:0005639"(integral component of nuclear inner membrane)|go:"GO:0005886"(plasma membrane)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0007268"(chemical synaptic transmission)|go:"GO:0007274"(neuromuscular synaptic transmission)|go:"GO:0007528"(neuromuscular junction development)|go:"GO:0007605"(sensory perception of sound)|go:"GO:0008270"(zinc ion binding)|go:"GO:0009743"(response to carbohydrate)|go:"GO:0009986"(cell surface)|go:"GO:0010033"(response to organic substance)|go:"GO:0014069"(postsynaptic density)|go:"GO:0014832"(urinary bladder smooth muscle contraction)|go:"GO:0015276"(ligand-gated ion channel activity)|go:"GO:0016020"(membrane)|go:"GO:0016021"(integral component of membrane)|go:"GO:0016151"(nickel cation binding)|go:"GO:0016324"(apical plasma membrane)|go:"GO:0019228"(neuronal action potential)|go:"GO:0030432"(peristalsis)|go:"GO:0033198"(response to ATP)|go:"GO:0035091"(phosphatidylinositol binding)|go:"GO:0042734"(presynaptic membrane)|go:"GO:0042802"(identical protein binding)|go:"GO:0043025"(neuronal cell body)|go:"GO:0043195"(terminal bouton)|go:"GO:0043197"(dendritic spine)|go:"GO:0043235"(receptor complex)|go:"GO:0044877"(protein-containing complex binding)|go:"GO:0045340"(mercury ion binding)|go:"GO:0046870"(cadmium ion binding)|go:"GO:0048266"(behavioral response to pain)|go:"GO:0048741"(skeletal muscle fiber development)|go:"GO:0050897"(cobalt ion binding)|go:"GO:0050909"(sensory perception of taste)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099059"(integral component of presynaptic active zone membrane)|go:"GO:0099509"(regulation of presynaptic cytosolic calcium ion concentration)|go:"GO:2000300"(regulation of synaptic vesicle exocytosis)|interpro:IPR001429(P2X purinoreceptor)|interpro:IPR003045(P2X2 purinoceptor)|interpro:IPR027309	rgd:62073|refseq:NP_113782.1|dip:DIP-48411N|ensembl:ENSRNOG00000001300(gene)|ensembl:ENSRNOT00000001752(transcript)|go:"GO:0001614"(purinergic nucleotide receptor activity)|go:"GO:0002028"(regulation of sodium ion transport)|go:"GO:0002931"(response to ischemia)|go:"GO:0004931"(extracellularly ATP-gated cation channel activity)|go:"GO:0005102"(signaling receptor binding)|go:"GO:0005507"(copper ion binding)|go:"GO:0005524"(ATP binding)|go:"GO:0005639"(integral component of nuclear inner membrane)|go:"GO:0005886"(plasma membrane)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0006809"(nitric oxide biosynthetic process)|go:"GO:0006816"(calcium ion transport)|go:"GO:0007165"(signal transduction)|go:"GO:0008217"(regulation of blood pressure)|go:"GO:0008270"(zinc ion binding)|go:"GO:0010524"(positive regulation of calcium ion transport into cytosol)|go:"GO:0010614"(negative regulation of cardiac muscle hypertrophy)|go:"GO:0014069"(postsynaptic density)|go:"GO:0016020"(membrane)|go:"GO:0019228"(neuronal action potential)|go:"GO:0019233"(sensory perception of pain)|go:"GO:0030054"(cell junction)|go:"GO:0030424"(axon)|go:"GO:0030425"(dendrite)|go:"GO:0033198"(response to ATP)|go:"GO:0034220"(ion transmembrane transport)|go:"GO:0034405"(response to fluid shear stress)|go:"GO:0035590"(purinergic nucleotide receptor signaling pathway)|go:"GO:0042311"(vasodilation)|go:"GO:0042802"(identical protein binding)|go:"GO:0043025"(neuronal cell body)|go:"GO:0043195"(terminal bouton)|go:"GO:0043197"(dendritic spine)|go:"GO:0043536"(positive regulation of blood vessel endothelial cell migration)|go:"GO:0044297"(cell body)|go:"GO:0045177"(apical part of cell)|go:"GO:0045202"(synapse)|go:"GO:0045296"(cadherin binding)|go:"GO:0048266"(behavioral response to pain)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0048678"(response to axon injury)|go:"GO:0050850"(positive regulation of calcium-mediated signaling)|go:"GO:0050920"(regulation of chemotaxis)|go:"GO:0050975"(sensory perception of touch)|go:"GO:0051897"(positive regulation of protein kinase B signaling)|go:"GO:0051899"(membrane depolarization)|go:"GO:0055117"(regulation of cardiac muscle contraction)|go:"GO:0055119"(relaxation of cardiac muscle)|go:"GO:0060079"(excitatory postsynaptic potential)|go:"GO:0070588"(calcium ion transmembrane transport)|go:"GO:0071294"(cellular response to zinc ion)|go:"GO:0071318"(cellular response to ATP)|go:"GO:0097190"(apoptotic signaling pathway)|go:"GO:0098662"(inorganic cation transmembrane transport)|go:"GO:0099604"(ligand-gated calcium channel activity)|go:"GO:1904141"(positive regulation of microglial cell migration)|go:"GO:2001028"(positive regulation of endothelial cell chemotaxis)|interpro:IPR001429(P2X purinoreceptor)|interpro:IPR003047(P2X4 purinoceptor)|interpro:IPR027309|rcsb pdb:2BP5|rcsb pdb:2RUP|reactome:R-RNO-139853|reactome:R-RNO-418346	-	-	-	figure legend:f1|comment:"Cells expressing various  combinations of P2X receptor subunits (P2X2/P2X4, P2X2/P2X6, P2X4/P2X6 and  P2X2/P2X4/P2X6) were fixed, permeabilized and incubated with appropriate primary  antibodies, followed by fluorophore-conjugated secondary antibodies (Sigma). Cells were  imaged by confocal laser scanning microscopy. As shown in Fig. 1, the immunostaining and  transfection patterns coincided exactly, and the absence of a particular subunit led to a  negative staining signal. The majority of co-transfected cells expressed each of the  transfected subunits, and in all cases the proteins appeared to be present throughout the cell."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:9606(human-tsa201)|taxid:9606(Homo sapiens subclone of human embryonic kidney 293 cell line that expresses simian virus 40 T antigen)	-	2014/04/28	2018/02/26	rogid:rHLp0w8RIFBzocD4Xjwd4vX7tJM10116	rogid:ogsHtvyxaAmmFUflijf6YfBuW7U10116	intact-crc:F6BE8378F380BA7C|rigid:xof/JpuCYhg9LAbOLA4Soop2ZMU	false	green fluorescent protein tag:?-?	ha tag:?-?	-	-	psi-mi:"MI:0867"(tag visualisation by fluorescence)	psi-mi:"MI:0707"(anti tag immunostaining)
uniprotkb:P49653	uniprotkb:P51579	intact:EBI-9511543|uniprotkb:O54868	intact:EBI-9511515|uniprotkb:R9PXR5|ensembl:ENSRNOP00000002558	psi-mi:p2rx2_rat(display_long)|uniprotkb:P2rx2(gene name)|psi-mi:P2rx2(display_short)|uniprotkb:ATP receptor(gene name synonym)|uniprotkb:Purinergic receptor(gene name synonym)	psi-mi:p2rx6_rat(display_long)|uniprotkb:P2rx6(gene name)|psi-mi:P2rx6(display_short)|uniprotkb:P2rxl1(gene name synonym)|uniprotkb:ATP receptor(gene name synonym)|uniprotkb:P2XM(gene name synonym)|uniprotkb:Purinergic receptor(gene name synonym)|uniprotkb:Purinergic receptor P2X-like 1(gene name synonym)	psi-mi:"MI:0416"(fluorescence microscopy)	Antonio et al. (2014)	pubmed:24815693|imex:IM-22591	taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)")	taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)")	psi-mi:"MI:0403"(colocalization)	psi-mi:"MI:0471"(MINT)	intact:EBI-9511670|imex:IM-22591-4	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	reactome:R-RNO-139853|reactome:R-RNO-418346|rgd:620251|refseq:NP_446108.2|dip:DIP-48329N|go:"GO:0001614"(purinergic nucleotide receptor activity)|go:"GO:0001666"(response to hypoxia)|go:"GO:0002931"(response to ischemia)|go:"GO:0003029"(detection of hypoxic conditions in blood by carotid body chemoreceptor signaling)|go:"GO:0004931"(extracellularly ATP-gated cation channel activity)|go:"GO:0005507"(copper ion binding)|go:"GO:0005524"(ATP binding)|go:"GO:0005639"(integral component of nuclear inner membrane)|go:"GO:0005886"(plasma membrane)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0007268"(chemical synaptic transmission)|go:"GO:0007274"(neuromuscular synaptic transmission)|go:"GO:0007528"(neuromuscular junction development)|go:"GO:0007605"(sensory perception of sound)|go:"GO:0008270"(zinc ion binding)|go:"GO:0009743"(response to carbohydrate)|go:"GO:0009986"(cell surface)|go:"GO:0010033"(response to organic substance)|go:"GO:0014069"(postsynaptic density)|go:"GO:0014832"(urinary bladder smooth muscle contraction)|go:"GO:0015276"(ligand-gated ion channel activity)|go:"GO:0016020"(membrane)|go:"GO:0016021"(integral component of membrane)|go:"GO:0016151"(nickel cation binding)|go:"GO:0016324"(apical plasma membrane)|go:"GO:0019228"(neuronal action potential)|go:"GO:0030432"(peristalsis)|go:"GO:0033198"(response to ATP)|go:"GO:0035091"(phosphatidylinositol binding)|go:"GO:0042734"(presynaptic membrane)|go:"GO:0042802"(identical protein binding)|go:"GO:0043025"(neuronal cell body)|go:"GO:0043195"(terminal bouton)|go:"GO:0043197"(dendritic spine)|go:"GO:0043235"(receptor complex)|go:"GO:0044877"(protein-containing complex binding)|go:"GO:0045340"(mercury ion binding)|go:"GO:0046870"(cadmium ion binding)|go:"GO:0048266"(behavioral response to pain)|go:"GO:0048741"(skeletal muscle fiber development)|go:"GO:0050897"(cobalt ion binding)|go:"GO:0050909"(sensory perception of taste)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099059"(integral component of presynaptic active zone membrane)|go:"GO:0099509"(regulation of presynaptic cytosolic calcium ion concentration)|go:"GO:2000300"(regulation of synaptic vesicle exocytosis)|interpro:IPR001429(P2X purinoreceptor)|interpro:IPR003045(P2X2 purinoceptor)|interpro:IPR027309	rgd:3243|refseq:NP_036853.2|ensembl:ENSRNOG00000001873(gene)|ensembl:ENSRNOT00000002558(transcript)|go:"GO:0001614"(purinergic nucleotide receptor activity)|go:"GO:0004931"(extracellularly ATP-gated cation channel activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005639"(integral component of nuclear inner membrane)|go:"GO:0005737"(cytoplasm)|go:"GO:0014069"(postsynaptic density)|go:"GO:0016020"(membrane)|go:"GO:0030054"(cell junction)|go:"GO:0033198"(response to ATP)|go:"GO:0043025"(neuronal cell body)|go:"GO:0043197"(dendritic spine)|go:"GO:0043235"(receptor complex)|go:"GO:0044877"(protein-containing complex binding)|go:"GO:0098688"(parallel fiber to Purkinje cell synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099060"(integral component of postsynaptic specialization membrane)|interpro:IPR001429(P2X purinoreceptor)|interpro:IPR003049(P2X6 purinoceptor)|interpro:IPR027309|reactome:R-RNO-139853|reactome:R-RNO-418346	-	-	-	figure legend:f1|comment:"Cells expressing various  combinations of P2X receptor subunits (P2X2/P2X4, P2X2/P2X6, P2X4/P2X6 and  P2X2/P2X4/P2X6) were fixed, permeabilized and incubated with appropriate primary  antibodies, followed by fluorophore-conjugated secondary antibodies (Sigma). Cells were  imaged by confocal laser scanning microscopy. As shown in Fig. 1, the immunostaining and  transfection patterns coincided exactly, and the absence of a particular subunit led to a  negative staining signal. The majority of co-transfected cells expressed each of the  transfected subunits, and in all cases the proteins appeared to be present throughout the cell."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:9606(human-tsa201)|taxid:9606(Homo sapiens subclone of human embryonic kidney 293 cell line that expresses simian virus 40 T antigen)	-	2014/04/28	2018/02/26	rogid:rHLp0w8RIFBzocD4Xjwd4vX7tJM10116	rogid:cTu+Raxv9pYr4tIXWDZiTfNHMl410116	intact-crc:F6BE8378F380BA7C|rigid:xof/JpuCYhg9LAbOLA4Soop2ZMU	false	green fluorescent protein tag:?-?	ha tag:?-?	-	-	psi-mi:"MI:0867"(tag visualisation by fluorescence)	psi-mi:"MI:0707"(anti tag immunostaining)
uniprotkb:P51577	uniprotkb:P49653	intact:EBI-9511617|ensembl:ENSRNOP00000001752	intact:EBI-9511543|uniprotkb:O54868	psi-mi:p2rx4_rat(display_long)|uniprotkb:P2rx4(gene name)|psi-mi:P2rx4(display_short)|uniprotkb:ATP receptor(gene name synonym)|uniprotkb:Purinergic receptor(gene name synonym)	psi-mi:p2rx2_rat(display_long)|uniprotkb:P2rx2(gene name)|psi-mi:P2rx2(display_short)|uniprotkb:ATP receptor(gene name synonym)|uniprotkb:Purinergic receptor(gene name synonym)	psi-mi:"MI:0416"(fluorescence microscopy)	Antonio et al. (2014)	pubmed:24815693|imex:IM-22591	taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)")	taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)")	psi-mi:"MI:0403"(colocalization)	psi-mi:"MI:0471"(MINT)	intact:EBI-9511658|imex:IM-22591-3	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	rgd:62073|refseq:NP_113782.1|dip:DIP-48411N|ensembl:ENSRNOG00000001300(gene)|ensembl:ENSRNOT00000001752(transcript)|go:"GO:0001614"(purinergic nucleotide receptor activity)|go:"GO:0002028"(regulation of sodium ion transport)|go:"GO:0002931"(response to ischemia)|go:"GO:0004931"(extracellularly ATP-gated cation channel activity)|go:"GO:0005102"(signaling receptor binding)|go:"GO:0005507"(copper ion binding)|go:"GO:0005524"(ATP binding)|go:"GO:0005639"(integral component of nuclear inner membrane)|go:"GO:0005886"(plasma membrane)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0006809"(nitric oxide biosynthetic process)|go:"GO:0006816"(calcium ion transport)|go:"GO:0007165"(signal transduction)|go:"GO:0008217"(regulation of blood pressure)|go:"GO:0008270"(zinc ion binding)|go:"GO:0010524"(positive regulation of calcium ion transport into cytosol)|go:"GO:0010614"(negative regulation of cardiac muscle hypertrophy)|go:"GO:0014069"(postsynaptic density)|go:"GO:0016020"(membrane)|go:"GO:0019228"(neuronal action potential)|go:"GO:0019233"(sensory perception of pain)|go:"GO:0030054"(cell junction)|go:"GO:0030424"(axon)|go:"GO:0030425"(dendrite)|go:"GO:0033198"(response to ATP)|go:"GO:0034220"(ion transmembrane transport)|go:"GO:0034405"(response to fluid shear stress)|go:"GO:0035590"(purinergic nucleotide receptor signaling pathway)|go:"GO:0042311"(vasodilation)|go:"GO:0042802"(identical protein binding)|go:"GO:0043025"(neuronal cell body)|go:"GO:0043195"(terminal bouton)|go:"GO:0043197"(dendritic spine)|go:"GO:0043536"(positive regulation of blood vessel endothelial cell migration)|go:"GO:0044297"(cell body)|go:"GO:0045177"(apical part of cell)|go:"GO:0045202"(synapse)|go:"GO:0045296"(cadherin binding)|go:"GO:0048266"(behavioral response to pain)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0048678"(response to axon injury)|go:"GO:0050850"(positive regulation of calcium-mediated signaling)|go:"GO:0050920"(regulation of chemotaxis)|go:"GO:0050975"(sensory perception of touch)|go:"GO:0051897"(positive regulation of protein kinase B signaling)|go:"GO:0051899"(membrane depolarization)|go:"GO:0055117"(regulation of cardiac muscle contraction)|go:"GO:0055119"(relaxation of cardiac muscle)|go:"GO:0060079"(excitatory postsynaptic potential)|go:"GO:0070588"(calcium ion transmembrane transport)|go:"GO:0071294"(cellular response to zinc ion)|go:"GO:0071318"(cellular response to ATP)|go:"GO:0097190"(apoptotic signaling pathway)|go:"GO:0098662"(inorganic cation transmembrane transport)|go:"GO:0099604"(ligand-gated calcium channel activity)|go:"GO:1904141"(positive regulation of microglial cell migration)|go:"GO:2001028"(positive regulation of endothelial cell chemotaxis)|interpro:IPR001429(P2X purinoreceptor)|interpro:IPR003047(P2X4 purinoceptor)|interpro:IPR027309|rcsb pdb:2BP5|rcsb pdb:2RUP|reactome:R-RNO-139853|reactome:R-RNO-418346	reactome:R-RNO-139853|reactome:R-RNO-418346|rgd:620251|refseq:NP_446108.2|dip:DIP-48329N|go:"GO:0001614"(purinergic nucleotide receptor activity)|go:"GO:0001666"(response to hypoxia)|go:"GO:0002931"(response to ischemia)|go:"GO:0003029"(detection of hypoxic conditions in blood by carotid body chemoreceptor signaling)|go:"GO:0004931"(extracellularly ATP-gated cation channel activity)|go:"GO:0005507"(copper ion binding)|go:"GO:0005524"(ATP binding)|go:"GO:0005639"(integral component of nuclear inner membrane)|go:"GO:0005886"(plasma membrane)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0007268"(chemical synaptic transmission)|go:"GO:0007274"(neuromuscular synaptic transmission)|go:"GO:0007528"(neuromuscular junction development)|go:"GO:0007605"(sensory perception of sound)|go:"GO:0008270"(zinc ion binding)|go:"GO:0009743"(response to carbohydrate)|go:"GO:0009986"(cell surface)|go:"GO:0010033"(response to organic substance)|go:"GO:0014069"(postsynaptic density)|go:"GO:0014832"(urinary bladder smooth muscle contraction)|go:"GO:0015276"(ligand-gated ion channel activity)|go:"GO:0016020"(membrane)|go:"GO:0016021"(integral component of membrane)|go:"GO:0016151"(nickel cation binding)|go:"GO:0016324"(apical plasma membrane)|go:"GO:0019228"(neuronal action potential)|go:"GO:0030432"(peristalsis)|go:"GO:0033198"(response to ATP)|go:"GO:0035091"(phosphatidylinositol binding)|go:"GO:0042734"(presynaptic membrane)|go:"GO:0042802"(identical protein binding)|go:"GO:0043025"(neuronal cell body)|go:"GO:0043195"(terminal bouton)|go:"GO:0043197"(dendritic spine)|go:"GO:0043235"(receptor complex)|go:"GO:0044877"(protein-containing complex binding)|go:"GO:0045340"(mercury ion binding)|go:"GO:0046870"(cadmium ion binding)|go:"GO:0048266"(behavioral response to pain)|go:"GO:0048741"(skeletal muscle fiber development)|go:"GO:0050897"(cobalt ion binding)|go:"GO:0050909"(sensory perception of taste)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099059"(integral component of presynaptic active zone membrane)|go:"GO:0099509"(regulation of presynaptic cytosolic calcium ion concentration)|go:"GO:2000300"(regulation of synaptic vesicle exocytosis)|interpro:IPR001429(P2X purinoreceptor)|interpro:IPR003045(P2X2 purinoceptor)|interpro:IPR027309	-	-	-	figure legend:f1|comment:"Cells expressing various  combinations of P2X receptor subunits (P2X2/P2X4, P2X2/P2X6, P2X4/P2X6 and  P2X2/P2X4/P2X6) were fixed, permeabilized and incubated with appropriate primary  antibodies, followed by fluorophore-conjugated secondary antibodies (Sigma). Cells were  imaged by confocal laser scanning microscopy. As shown in Fig. 1, the immunostaining and  transfection patterns coincided exactly, and the absence of a particular subunit led to a  negative staining signal. The majority of co-transfected cells expressed each of the  transfected subunits, and in all cases the proteins appeared to be present throughout the cell."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:9606(human-tsa201)|taxid:9606(Homo sapiens subclone of human embryonic kidney 293 cell line that expresses simian virus 40 T antigen)	-	2014/04/28	2014/10/16	rogid:ogsHtvyxaAmmFUflijf6YfBuW7U10116	rogid:rHLp0w8RIFBzocD4Xjwd4vX7tJM10116	intact-crc:984A3E65BE54EB64|rigid:X2pWxKyymRGNVBG4TspcQwsM54M	false	ha tag:?-?	green fluorescent protein tag:?-?	-	-	psi-mi:"MI:0707"(anti tag immunostaining)	psi-mi:"MI:0867"(tag visualisation by fluorescence)
uniprotkb:P49653	uniprotkb:P51579	intact:EBI-9511543|uniprotkb:O54868	intact:EBI-9511515|uniprotkb:R9PXR5|ensembl:ENSRNOP00000002558	psi-mi:p2rx2_rat(display_long)|uniprotkb:P2rx2(gene name)|psi-mi:P2rx2(display_short)|uniprotkb:ATP receptor(gene name synonym)|uniprotkb:Purinergic receptor(gene name synonym)	psi-mi:p2rx6_rat(display_long)|uniprotkb:P2rx6(gene name)|psi-mi:P2rx6(display_short)|uniprotkb:P2rxl1(gene name synonym)|uniprotkb:ATP receptor(gene name synonym)|uniprotkb:P2XM(gene name synonym)|uniprotkb:Purinergic receptor(gene name synonym)|uniprotkb:Purinergic receptor P2X-like 1(gene name synonym)	psi-mi:"MI:0416"(fluorescence microscopy)	Antonio et al. (2014)	pubmed:24815693|imex:IM-22591	taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)")	taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)")	psi-mi:"MI:0403"(colocalization)	psi-mi:"MI:0471"(MINT)	intact:EBI-9511452|imex:IM-22591-1	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	reactome:R-RNO-139853|reactome:R-RNO-418346|rgd:620251|refseq:NP_446108.2|dip:DIP-48329N|go:"GO:0001614"(purinergic nucleotide receptor activity)|go:"GO:0001666"(response to hypoxia)|go:"GO:0002931"(response to ischemia)|go:"GO:0003029"(detection of hypoxic conditions in blood by carotid body chemoreceptor signaling)|go:"GO:0004931"(extracellularly ATP-gated cation channel activity)|go:"GO:0005507"(copper ion binding)|go:"GO:0005524"(ATP binding)|go:"GO:0005639"(integral component of nuclear inner membrane)|go:"GO:0005886"(plasma membrane)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0007268"(chemical synaptic transmission)|go:"GO:0007274"(neuromuscular synaptic transmission)|go:"GO:0007528"(neuromuscular junction development)|go:"GO:0007605"(sensory perception of sound)|go:"GO:0008270"(zinc ion binding)|go:"GO:0009743"(response to carbohydrate)|go:"GO:0009986"(cell surface)|go:"GO:0010033"(response to organic substance)|go:"GO:0014069"(postsynaptic density)|go:"GO:0014832"(urinary bladder smooth muscle contraction)|go:"GO:0015276"(ligand-gated ion channel activity)|go:"GO:0016020"(membrane)|go:"GO:0016021"(integral component of membrane)|go:"GO:0016151"(nickel cation binding)|go:"GO:0016324"(apical plasma membrane)|go:"GO:0019228"(neuronal action potential)|go:"GO:0030432"(peristalsis)|go:"GO:0033198"(response to ATP)|go:"GO:0035091"(phosphatidylinositol binding)|go:"GO:0042734"(presynaptic membrane)|go:"GO:0042802"(identical protein binding)|go:"GO:0043025"(neuronal cell body)|go:"GO:0043195"(terminal bouton)|go:"GO:0043197"(dendritic spine)|go:"GO:0043235"(receptor complex)|go:"GO:0044877"(protein-containing complex binding)|go:"GO:0045340"(mercury ion binding)|go:"GO:0046870"(cadmium ion binding)|go:"GO:0048266"(behavioral response to pain)|go:"GO:0048741"(skeletal muscle fiber development)|go:"GO:0050897"(cobalt ion binding)|go:"GO:0050909"(sensory perception of taste)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099059"(integral component of presynaptic active zone membrane)|go:"GO:0099509"(regulation of presynaptic cytosolic calcium ion concentration)|go:"GO:2000300"(regulation of synaptic vesicle exocytosis)|interpro:IPR001429(P2X purinoreceptor)|interpro:IPR003045(P2X2 purinoceptor)|interpro:IPR027309	rgd:3243|refseq:NP_036853.2|ensembl:ENSRNOG00000001873(gene)|ensembl:ENSRNOT00000002558(transcript)|go:"GO:0001614"(purinergic nucleotide receptor activity)|go:"GO:0004931"(extracellularly ATP-gated cation channel activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005639"(integral component of nuclear inner membrane)|go:"GO:0005737"(cytoplasm)|go:"GO:0014069"(postsynaptic density)|go:"GO:0016020"(membrane)|go:"GO:0030054"(cell junction)|go:"GO:0033198"(response to ATP)|go:"GO:0043025"(neuronal cell body)|go:"GO:0043197"(dendritic spine)|go:"GO:0043235"(receptor complex)|go:"GO:0044877"(protein-containing complex binding)|go:"GO:0098688"(parallel fiber to Purkinje cell synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099060"(integral component of postsynaptic specialization membrane)|interpro:IPR001429(P2X purinoreceptor)|interpro:IPR003049(P2X6 purinoceptor)|interpro:IPR027309|reactome:R-RNO-139853|reactome:R-RNO-418346	-	-	-	figure legend:f1|comment:"Cells expressing various  combinations of P2X receptor subunits (P2X2/P2X4, P2X2/P2X6, P2X4/P2X6 and  P2X2/P2X4/P2X6) were fixed, permeabilized and incubated with appropriate primary  antibodies, followed by fluorophore-conjugated secondary antibodies (Sigma). Cells were  imaged by confocal laser scanning microscopy. As shown in Fig. 1, the immunostaining and  transfection patterns coincided exactly, and the absence of a particular subunit led to a  negative staining signal. The majority of co-transfected cells expressed each of the  transfected subunits, and in all cases the proteins appeared to be present throughout the cell."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:9606(human-tsa201)|taxid:9606(Homo sapiens subclone of human embryonic kidney 293 cell line that expresses simian virus 40 T antigen)	-	2014/04/28	2018/02/26	rogid:rHLp0w8RIFBzocD4Xjwd4vX7tJM10116	rogid:cTu+Raxv9pYr4tIXWDZiTfNHMl410116	intact-crc:6A03EA907B4424B1|rigid:Ruzu0FN+lFKVxXmLcIJzywAssFc	false	green fluorescent protein tag:?-?	his tag:?-?	-	-	psi-mi:"MI:0867"(tag visualisation by fluorescence)	psi-mi:"MI:0707"(anti tag immunostaining)
uniprotkb:P51577	uniprotkb:P51579	intact:EBI-9511617|ensembl:ENSRNOP00000001752	intact:EBI-9511515|uniprotkb:R9PXR5|ensembl:ENSRNOP00000002558	psi-mi:p2rx4_rat(display_long)|uniprotkb:P2rx4(gene name)|psi-mi:P2rx4(display_short)|uniprotkb:ATP receptor(gene name synonym)|uniprotkb:Purinergic receptor(gene name synonym)	psi-mi:p2rx6_rat(display_long)|uniprotkb:P2rx6(gene name)|psi-mi:P2rx6(display_short)|uniprotkb:P2rxl1(gene name synonym)|uniprotkb:ATP receptor(gene name synonym)|uniprotkb:P2XM(gene name synonym)|uniprotkb:Purinergic receptor(gene name synonym)|uniprotkb:Purinergic receptor P2X-like 1(gene name synonym)	psi-mi:"MI:0007"(anti tag coimmunoprecipitation)	Antonio et al. (2014)	pubmed:24815693|imex:IM-22591	taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)")	taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)")	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-9511757|imex:IM-22591-8	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	rgd:62073|refseq:NP_113782.1|dip:DIP-48411N|ensembl:ENSRNOG00000001300(gene)|ensembl:ENSRNOT00000001752(transcript)|go:"GO:0001614"(purinergic nucleotide receptor activity)|go:"GO:0002028"(regulation of sodium ion transport)|go:"GO:0002931"(response to ischemia)|go:"GO:0004931"(extracellularly ATP-gated cation channel activity)|go:"GO:0005102"(signaling receptor binding)|go:"GO:0005507"(copper ion binding)|go:"GO:0005524"(ATP binding)|go:"GO:0005639"(integral component of nuclear inner membrane)|go:"GO:0005886"(plasma membrane)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0006809"(nitric oxide biosynthetic process)|go:"GO:0006816"(calcium ion transport)|go:"GO:0007165"(signal transduction)|go:"GO:0008217"(regulation of blood pressure)|go:"GO:0008270"(zinc ion binding)|go:"GO:0010524"(positive regulation of calcium ion transport into cytosol)|go:"GO:0010614"(negative regulation of cardiac muscle hypertrophy)|go:"GO:0014069"(postsynaptic density)|go:"GO:0016020"(membrane)|go:"GO:0019228"(neuronal action potential)|go:"GO:0019233"(sensory perception of pain)|go:"GO:0030054"(cell junction)|go:"GO:0030424"(axon)|go:"GO:0030425"(dendrite)|go:"GO:0033198"(response to ATP)|go:"GO:0034220"(ion transmembrane transport)|go:"GO:0034405"(response to fluid shear stress)|go:"GO:0035590"(purinergic nucleotide receptor signaling pathway)|go:"GO:0042311"(vasodilation)|go:"GO:0042802"(identical protein binding)|go:"GO:0043025"(neuronal cell body)|go:"GO:0043195"(terminal bouton)|go:"GO:0043197"(dendritic spine)|go:"GO:0043536"(positive regulation of blood vessel endothelial cell migration)|go:"GO:0044297"(cell body)|go:"GO:0045177"(apical part of cell)|go:"GO:0045202"(synapse)|go:"GO:0045296"(cadherin binding)|go:"GO:0048266"(behavioral response to pain)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0048678"(response to axon injury)|go:"GO:0050850"(positive regulation of calcium-mediated signaling)|go:"GO:0050920"(regulation of chemotaxis)|go:"GO:0050975"(sensory perception of touch)|go:"GO:0051897"(positive regulation of protein kinase B signaling)|go:"GO:0051899"(membrane depolarization)|go:"GO:0055117"(regulation of cardiac muscle contraction)|go:"GO:0055119"(relaxation of cardiac muscle)|go:"GO:0060079"(excitatory postsynaptic potential)|go:"GO:0070588"(calcium ion transmembrane transport)|go:"GO:0071294"(cellular response to zinc ion)|go:"GO:0071318"(cellular response to ATP)|go:"GO:0097190"(apoptotic signaling pathway)|go:"GO:0098662"(inorganic cation transmembrane transport)|go:"GO:0099604"(ligand-gated calcium channel activity)|go:"GO:1904141"(positive regulation of microglial cell migration)|go:"GO:2001028"(positive regulation of endothelial cell chemotaxis)|interpro:IPR001429(P2X purinoreceptor)|interpro:IPR003047(P2X4 purinoceptor)|interpro:IPR027309|rcsb pdb:2BP5|rcsb pdb:2RUP|reactome:R-RNO-139853|reactome:R-RNO-418346	rgd:3243|refseq:NP_036853.2|ensembl:ENSRNOG00000001873(gene)|ensembl:ENSRNOT00000002558(transcript)|go:"GO:0001614"(purinergic nucleotide receptor activity)|go:"GO:0004931"(extracellularly ATP-gated cation channel activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005639"(integral component of nuclear inner membrane)|go:"GO:0005737"(cytoplasm)|go:"GO:0014069"(postsynaptic density)|go:"GO:0016020"(membrane)|go:"GO:0030054"(cell junction)|go:"GO:0033198"(response to ATP)|go:"GO:0043025"(neuronal cell body)|go:"GO:0043197"(dendritic spine)|go:"GO:0043235"(receptor complex)|go:"GO:0044877"(protein-containing complex binding)|go:"GO:0098688"(parallel fiber to Purkinje cell synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099060"(integral component of postsynaptic specialization membrane)|interpro:IPR001429(P2X purinoreceptor)|interpro:IPR003049(P2X6 purinoceptor)|interpro:IPR027309|reactome:R-RNO-139853|reactome:R-RNO-418346	-	-	-	figure legend:f2|comment:"A detergent extract of cells co-expressing His6-P2X2, P2X4-HA and FLAG-P2X6 was  subjected to sequential anti-HA and anti-FLAG immunoaffinity chromatography steps (Fig.  2). The first (anti-HA) step would be expected capture any protein complex containing P2X4-  HA, while the second (anti-FLAG) step would capture complexes containing both P2X4-HA  and FLAG-P2X6. The immunoblots showed that this was indeed the case, and also indicated  that His6-P2X2 was also present in the second eluate. The same result was obtained when the  8  order of the immunoaffinity chromatography steps was reversed. Fig. 2 also shows that when  either FLAG-P2X6 or P2X4-HA was omitted, none of the three subunits appeared in the  second eluate. These results indicate an intimate association between P2X2, P2X4 and P2X6."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:9606(human-tsa201)|taxid:9606(Homo sapiens subclone of human embryonic kidney 293 cell line that expresses simian virus 40 T antigen)	-	2014/04/28	2018/02/26	rogid:ogsHtvyxaAmmFUflijf6YfBuW7U10116	rogid:cTu+Raxv9pYr4tIXWDZiTfNHMl410116	intact-crc:66E2366741805404|rigid:xof/JpuCYhg9LAbOLA4Soop2ZMU	false	ha tag:?-?	flag tag:?-?	-	-	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)
uniprotkb:P51577	uniprotkb:P49653	intact:EBI-9511617|ensembl:ENSRNOP00000001752	intact:EBI-9511543|uniprotkb:O54868	psi-mi:p2rx4_rat(display_long)|uniprotkb:P2rx4(gene name)|psi-mi:P2rx4(display_short)|uniprotkb:ATP receptor(gene name synonym)|uniprotkb:Purinergic receptor(gene name synonym)	psi-mi:p2rx2_rat(display_long)|uniprotkb:P2rx2(gene name)|psi-mi:P2rx2(display_short)|uniprotkb:ATP receptor(gene name synonym)|uniprotkb:Purinergic receptor(gene name synonym)	psi-mi:"MI:0007"(anti tag coimmunoprecipitation)	Antonio et al. (2014)	pubmed:24815693|imex:IM-22591	taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)")	taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)")	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-9511757|imex:IM-22591-8	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	rgd:62073|refseq:NP_113782.1|dip:DIP-48411N|ensembl:ENSRNOG00000001300(gene)|ensembl:ENSRNOT00000001752(transcript)|go:"GO:0001614"(purinergic nucleotide receptor activity)|go:"GO:0002028"(regulation of sodium ion transport)|go:"GO:0002931"(response to ischemia)|go:"GO:0004931"(extracellularly ATP-gated cation channel activity)|go:"GO:0005102"(signaling receptor binding)|go:"GO:0005507"(copper ion binding)|go:"GO:0005524"(ATP binding)|go:"GO:0005639"(integral component of nuclear inner membrane)|go:"GO:0005886"(plasma membrane)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0006809"(nitric oxide biosynthetic process)|go:"GO:0006816"(calcium ion transport)|go:"GO:0007165"(signal transduction)|go:"GO:0008217"(regulation of blood pressure)|go:"GO:0008270"(zinc ion binding)|go:"GO:0010524"(positive regulation of calcium ion transport into cytosol)|go:"GO:0010614"(negative regulation of cardiac muscle hypertrophy)|go:"GO:0014069"(postsynaptic density)|go:"GO:0016020"(membrane)|go:"GO:0019228"(neuronal action potential)|go:"GO:0019233"(sensory perception of pain)|go:"GO:0030054"(cell junction)|go:"GO:0030424"(axon)|go:"GO:0030425"(dendrite)|go:"GO:0033198"(response to ATP)|go:"GO:0034220"(ion transmembrane transport)|go:"GO:0034405"(response to fluid shear stress)|go:"GO:0035590"(purinergic nucleotide receptor signaling pathway)|go:"GO:0042311"(vasodilation)|go:"GO:0042802"(identical protein binding)|go:"GO:0043025"(neuronal cell body)|go:"GO:0043195"(terminal bouton)|go:"GO:0043197"(dendritic spine)|go:"GO:0043536"(positive regulation of blood vessel endothelial cell migration)|go:"GO:0044297"(cell body)|go:"GO:0045177"(apical part of cell)|go:"GO:0045202"(synapse)|go:"GO:0045296"(cadherin binding)|go:"GO:0048266"(behavioral response to pain)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0048678"(response to axon injury)|go:"GO:0050850"(positive regulation of calcium-mediated signaling)|go:"GO:0050920"(regulation of chemotaxis)|go:"GO:0050975"(sensory perception of touch)|go:"GO:0051897"(positive regulation of protein kinase B signaling)|go:"GO:0051899"(membrane depolarization)|go:"GO:0055117"(regulation of cardiac muscle contraction)|go:"GO:0055119"(relaxation of cardiac muscle)|go:"GO:0060079"(excitatory postsynaptic potential)|go:"GO:0070588"(calcium ion transmembrane transport)|go:"GO:0071294"(cellular response to zinc ion)|go:"GO:0071318"(cellular response to ATP)|go:"GO:0097190"(apoptotic signaling pathway)|go:"GO:0098662"(inorganic cation transmembrane transport)|go:"GO:0099604"(ligand-gated calcium channel activity)|go:"GO:1904141"(positive regulation of microglial cell migration)|go:"GO:2001028"(positive regulation of endothelial cell chemotaxis)|interpro:IPR001429(P2X purinoreceptor)|interpro:IPR003047(P2X4 purinoceptor)|interpro:IPR027309|rcsb pdb:2BP5|rcsb pdb:2RUP|reactome:R-RNO-139853|reactome:R-RNO-418346	reactome:R-RNO-139853|reactome:R-RNO-418346|rgd:620251|refseq:NP_446108.2|dip:DIP-48329N|go:"GO:0001614"(purinergic nucleotide receptor activity)|go:"GO:0001666"(response to hypoxia)|go:"GO:0002931"(response to ischemia)|go:"GO:0003029"(detection of hypoxic conditions in blood by carotid body chemoreceptor signaling)|go:"GO:0004931"(extracellularly ATP-gated cation channel activity)|go:"GO:0005507"(copper ion binding)|go:"GO:0005524"(ATP binding)|go:"GO:0005639"(integral component of nuclear inner membrane)|go:"GO:0005886"(plasma membrane)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0007268"(chemical synaptic transmission)|go:"GO:0007274"(neuromuscular synaptic transmission)|go:"GO:0007528"(neuromuscular junction development)|go:"GO:0007605"(sensory perception of sound)|go:"GO:0008270"(zinc ion binding)|go:"GO:0009743"(response to carbohydrate)|go:"GO:0009986"(cell surface)|go:"GO:0010033"(response to organic substance)|go:"GO:0014069"(postsynaptic density)|go:"GO:0014832"(urinary bladder smooth muscle contraction)|go:"GO:0015276"(ligand-gated ion channel activity)|go:"GO:0016020"(membrane)|go:"GO:0016021"(integral component of membrane)|go:"GO:0016151"(nickel cation binding)|go:"GO:0016324"(apical plasma membrane)|go:"GO:0019228"(neuronal action potential)|go:"GO:0030432"(peristalsis)|go:"GO:0033198"(response to ATP)|go:"GO:0035091"(phosphatidylinositol binding)|go:"GO:0042734"(presynaptic membrane)|go:"GO:0042802"(identical protein binding)|go:"GO:0043025"(neuronal cell body)|go:"GO:0043195"(terminal bouton)|go:"GO:0043197"(dendritic spine)|go:"GO:0043235"(receptor complex)|go:"GO:0044877"(protein-containing complex binding)|go:"GO:0045340"(mercury ion binding)|go:"GO:0046870"(cadmium ion binding)|go:"GO:0048266"(behavioral response to pain)|go:"GO:0048741"(skeletal muscle fiber development)|go:"GO:0050897"(cobalt ion binding)|go:"GO:0050909"(sensory perception of taste)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099059"(integral component of presynaptic active zone membrane)|go:"GO:0099509"(regulation of presynaptic cytosolic calcium ion concentration)|go:"GO:2000300"(regulation of synaptic vesicle exocytosis)|interpro:IPR001429(P2X purinoreceptor)|interpro:IPR003045(P2X2 purinoceptor)|interpro:IPR027309	-	-	-	figure legend:f2|comment:"A detergent extract of cells co-expressing His6-P2X2, P2X4-HA and FLAG-P2X6 was  subjected to sequential anti-HA and anti-FLAG immunoaffinity chromatography steps (Fig.  2). The first (anti-HA) step would be expected capture any protein complex containing P2X4-  HA, while the second (anti-FLAG) step would capture complexes containing both P2X4-HA  and FLAG-P2X6. The immunoblots showed that this was indeed the case, and also indicated  that His6-P2X2 was also present in the second eluate. The same result was obtained when the  8  order of the immunoaffinity chromatography steps was reversed. Fig. 2 also shows that when  either FLAG-P2X6 or P2X4-HA was omitted, none of the three subunits appeared in the  second eluate. These results indicate an intimate association between P2X2, P2X4 and P2X6."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:9606(human-tsa201)|taxid:9606(Homo sapiens subclone of human embryonic kidney 293 cell line that expresses simian virus 40 T antigen)	-	2014/04/28	2018/02/26	rogid:ogsHtvyxaAmmFUflijf6YfBuW7U10116	rogid:rHLp0w8RIFBzocD4Xjwd4vX7tJM10116	intact-crc:66E2366741805404|rigid:xof/JpuCYhg9LAbOLA4Soop2ZMU	false	ha tag:?-?	his tag:?-?	-	-	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)
uniprotkb:P49653	uniprotkb:P51579	intact:EBI-9511543|uniprotkb:O54868	intact:EBI-9511515|uniprotkb:R9PXR5|ensembl:ENSRNOP00000002558	psi-mi:p2rx2_rat(display_long)|uniprotkb:P2rx2(gene name)|psi-mi:P2rx2(display_short)|uniprotkb:ATP receptor(gene name synonym)|uniprotkb:Purinergic receptor(gene name synonym)	psi-mi:p2rx6_rat(display_long)|uniprotkb:P2rx6(gene name)|psi-mi:P2rx6(display_short)|uniprotkb:P2rxl1(gene name synonym)|uniprotkb:ATP receptor(gene name synonym)|uniprotkb:P2XM(gene name synonym)|uniprotkb:Purinergic receptor(gene name synonym)|uniprotkb:Purinergic receptor P2X-like 1(gene name synonym)	psi-mi:"MI:0007"(anti tag coimmunoprecipitation)	Antonio et al. (2014)	pubmed:24815693|imex:IM-22591	taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)")	taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)")	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0471"(MINT)	intact:EBI-9511691|imex:IM-22591-5	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	reactome:R-RNO-139853|reactome:R-RNO-418346|rgd:620251|refseq:NP_446108.2|dip:DIP-48329N|go:"GO:0001614"(purinergic nucleotide receptor activity)|go:"GO:0001666"(response to hypoxia)|go:"GO:0002931"(response to ischemia)|go:"GO:0003029"(detection of hypoxic conditions in blood by carotid body chemoreceptor signaling)|go:"GO:0004931"(extracellularly ATP-gated cation channel activity)|go:"GO:0005507"(copper ion binding)|go:"GO:0005524"(ATP binding)|go:"GO:0005639"(integral component of nuclear inner membrane)|go:"GO:0005886"(plasma membrane)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0007268"(chemical synaptic transmission)|go:"GO:0007274"(neuromuscular synaptic transmission)|go:"GO:0007528"(neuromuscular junction development)|go:"GO:0007605"(sensory perception of sound)|go:"GO:0008270"(zinc ion binding)|go:"GO:0009743"(response to carbohydrate)|go:"GO:0009986"(cell surface)|go:"GO:0010033"(response to organic substance)|go:"GO:0014069"(postsynaptic density)|go:"GO:0014832"(urinary bladder smooth muscle contraction)|go:"GO:0015276"(ligand-gated ion channel activity)|go:"GO:0016020"(membrane)|go:"GO:0016021"(integral component of membrane)|go:"GO:0016151"(nickel cation binding)|go:"GO:0016324"(apical plasma membrane)|go:"GO:0019228"(neuronal action potential)|go:"GO:0030432"(peristalsis)|go:"GO:0033198"(response to ATP)|go:"GO:0035091"(phosphatidylinositol binding)|go:"GO:0042734"(presynaptic membrane)|go:"GO:0042802"(identical protein binding)|go:"GO:0043025"(neuronal cell body)|go:"GO:0043195"(terminal bouton)|go:"GO:0043197"(dendritic spine)|go:"GO:0043235"(receptor complex)|go:"GO:0044877"(protein-containing complex binding)|go:"GO:0045340"(mercury ion binding)|go:"GO:0046870"(cadmium ion binding)|go:"GO:0048266"(behavioral response to pain)|go:"GO:0048741"(skeletal muscle fiber development)|go:"GO:0050897"(cobalt ion binding)|go:"GO:0050909"(sensory perception of taste)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099059"(integral component of presynaptic active zone membrane)|go:"GO:0099509"(regulation of presynaptic cytosolic calcium ion concentration)|go:"GO:2000300"(regulation of synaptic vesicle exocytosis)|interpro:IPR001429(P2X purinoreceptor)|interpro:IPR003045(P2X2 purinoceptor)|interpro:IPR027309	rgd:3243|refseq:NP_036853.2|ensembl:ENSRNOG00000001873(gene)|ensembl:ENSRNOT00000002558(transcript)|go:"GO:0001614"(purinergic nucleotide receptor activity)|go:"GO:0004931"(extracellularly ATP-gated cation channel activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005639"(integral component of nuclear inner membrane)|go:"GO:0005737"(cytoplasm)|go:"GO:0014069"(postsynaptic density)|go:"GO:0016020"(membrane)|go:"GO:0030054"(cell junction)|go:"GO:0033198"(response to ATP)|go:"GO:0043025"(neuronal cell body)|go:"GO:0043197"(dendritic spine)|go:"GO:0043235"(receptor complex)|go:"GO:0044877"(protein-containing complex binding)|go:"GO:0098688"(parallel fiber to Purkinje cell synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099060"(integral component of postsynaptic specialization membrane)|interpro:IPR001429(P2X purinoreceptor)|interpro:IPR003049(P2X6 purinoceptor)|interpro:IPR027309|reactome:R-RNO-139853|reactome:R-RNO-418346	-	-	-	figure legend:f2|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:9606(human-tsa201)|taxid:9606(Homo sapiens subclone of human embryonic kidney 293 cell line that expresses simian virus 40 T antigen)	-	2014/04/28	2018/02/26	rogid:rHLp0w8RIFBzocD4Xjwd4vX7tJM10116	rogid:cTu+Raxv9pYr4tIXWDZiTfNHMl410116	intact-crc:927ECF6EF36288F2|rigid:Ruzu0FN+lFKVxXmLcIJzywAssFc	false	his tag:?-?	flag tag:?-?	-	-	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)
uniprotkb:P49653	uniprotkb:P51577	intact:EBI-9511543|uniprotkb:O54868	intact:EBI-9511617|ensembl:ENSRNOP00000001752	psi-mi:p2rx2_rat(display_long)|uniprotkb:P2rx2(gene name)|psi-mi:P2rx2(display_short)|uniprotkb:ATP receptor(gene name synonym)|uniprotkb:Purinergic receptor(gene name synonym)	psi-mi:p2rx4_rat(display_long)|uniprotkb:P2rx4(gene name)|psi-mi:P2rx4(display_short)|uniprotkb:ATP receptor(gene name synonym)|uniprotkb:Purinergic receptor(gene name synonym)	psi-mi:"MI:0007"(anti tag coimmunoprecipitation)	Antonio et al. (2014)	pubmed:24815693|imex:IM-22591	taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)")	taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)")	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0471"(MINT)	intact:EBI-9511732|imex:IM-22591-6	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	reactome:R-RNO-139853|reactome:R-RNO-418346|rgd:620251|refseq:NP_446108.2|dip:DIP-48329N|go:"GO:0001614"(purinergic nucleotide receptor activity)|go:"GO:0001666"(response to hypoxia)|go:"GO:0002931"(response to ischemia)|go:"GO:0003029"(detection of hypoxic conditions in blood by carotid body chemoreceptor signaling)|go:"GO:0004931"(extracellularly ATP-gated cation channel activity)|go:"GO:0005507"(copper ion binding)|go:"GO:0005524"(ATP binding)|go:"GO:0005639"(integral component of nuclear inner membrane)|go:"GO:0005886"(plasma membrane)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0007268"(chemical synaptic transmission)|go:"GO:0007274"(neuromuscular synaptic transmission)|go:"GO:0007528"(neuromuscular junction development)|go:"GO:0007605"(sensory perception of sound)|go:"GO:0008270"(zinc ion binding)|go:"GO:0009743"(response to carbohydrate)|go:"GO:0009986"(cell surface)|go:"GO:0010033"(response to organic substance)|go:"GO:0014069"(postsynaptic density)|go:"GO:0014832"(urinary bladder smooth muscle contraction)|go:"GO:0015276"(ligand-gated ion channel activity)|go:"GO:0016020"(membrane)|go:"GO:0016021"(integral component of membrane)|go:"GO:0016151"(nickel cation binding)|go:"GO:0016324"(apical plasma membrane)|go:"GO:0019228"(neuronal action potential)|go:"GO:0030432"(peristalsis)|go:"GO:0033198"(response to ATP)|go:"GO:0035091"(phosphatidylinositol binding)|go:"GO:0042734"(presynaptic membrane)|go:"GO:0042802"(identical protein binding)|go:"GO:0043025"(neuronal cell body)|go:"GO:0043195"(terminal bouton)|go:"GO:0043197"(dendritic spine)|go:"GO:0043235"(receptor complex)|go:"GO:0044877"(protein-containing complex binding)|go:"GO:0045340"(mercury ion binding)|go:"GO:0046870"(cadmium ion binding)|go:"GO:0048266"(behavioral response to pain)|go:"GO:0048741"(skeletal muscle fiber development)|go:"GO:0050897"(cobalt ion binding)|go:"GO:0050909"(sensory perception of taste)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099059"(integral component of presynaptic active zone membrane)|go:"GO:0099509"(regulation of presynaptic cytosolic calcium ion concentration)|go:"GO:2000300"(regulation of synaptic vesicle exocytosis)|interpro:IPR001429(P2X purinoreceptor)|interpro:IPR003045(P2X2 purinoceptor)|interpro:IPR027309	rgd:62073|refseq:NP_113782.1|dip:DIP-48411N|ensembl:ENSRNOG00000001300(gene)|ensembl:ENSRNOT00000001752(transcript)|go:"GO:0001614"(purinergic nucleotide receptor activity)|go:"GO:0002028"(regulation of sodium ion transport)|go:"GO:0002931"(response to ischemia)|go:"GO:0004931"(extracellularly ATP-gated cation channel activity)|go:"GO:0005102"(signaling receptor binding)|go:"GO:0005507"(copper ion binding)|go:"GO:0005524"(ATP binding)|go:"GO:0005639"(integral component of nuclear inner membrane)|go:"GO:0005886"(plasma membrane)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0006809"(nitric oxide biosynthetic process)|go:"GO:0006816"(calcium ion transport)|go:"GO:0007165"(signal transduction)|go:"GO:0008217"(regulation of blood pressure)|go:"GO:0008270"(zinc ion binding)|go:"GO:0010524"(positive regulation of calcium ion transport into cytosol)|go:"GO:0010614"(negative regulation of cardiac muscle hypertrophy)|go:"GO:0014069"(postsynaptic density)|go:"GO:0016020"(membrane)|go:"GO:0019228"(neuronal action potential)|go:"GO:0019233"(sensory perception of pain)|go:"GO:0030054"(cell junction)|go:"GO:0030424"(axon)|go:"GO:0030425"(dendrite)|go:"GO:0033198"(response to ATP)|go:"GO:0034220"(ion transmembrane transport)|go:"GO:0034405"(response to fluid shear stress)|go:"GO:0035590"(purinergic nucleotide receptor signaling pathway)|go:"GO:0042311"(vasodilation)|go:"GO:0042802"(identical protein binding)|go:"GO:0043025"(neuronal cell body)|go:"GO:0043195"(terminal bouton)|go:"GO:0043197"(dendritic spine)|go:"GO:0043536"(positive regulation of blood vessel endothelial cell migration)|go:"GO:0044297"(cell body)|go:"GO:0045177"(apical part of cell)|go:"GO:0045202"(synapse)|go:"GO:0045296"(cadherin binding)|go:"GO:0048266"(behavioral response to pain)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0048678"(response to axon injury)|go:"GO:0050850"(positive regulation of calcium-mediated signaling)|go:"GO:0050920"(regulation of chemotaxis)|go:"GO:0050975"(sensory perception of touch)|go:"GO:0051897"(positive regulation of protein kinase B signaling)|go:"GO:0051899"(membrane depolarization)|go:"GO:0055117"(regulation of cardiac muscle contraction)|go:"GO:0055119"(relaxation of cardiac muscle)|go:"GO:0060079"(excitatory postsynaptic potential)|go:"GO:0070588"(calcium ion transmembrane transport)|go:"GO:0071294"(cellular response to zinc ion)|go:"GO:0071318"(cellular response to ATP)|go:"GO:0097190"(apoptotic signaling pathway)|go:"GO:0098662"(inorganic cation transmembrane transport)|go:"GO:0099604"(ligand-gated calcium channel activity)|go:"GO:1904141"(positive regulation of microglial cell migration)|go:"GO:2001028"(positive regulation of endothelial cell chemotaxis)|interpro:IPR001429(P2X purinoreceptor)|interpro:IPR003047(P2X4 purinoceptor)|interpro:IPR027309|rcsb pdb:2BP5|rcsb pdb:2RUP|reactome:R-RNO-139853|reactome:R-RNO-418346	-	-	-	figure legend:f2|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:9606(human-tsa201)|taxid:9606(Homo sapiens subclone of human embryonic kidney 293 cell line that expresses simian virus 40 T antigen)	-	2014/04/28	2014/10/16	rogid:rHLp0w8RIFBzocD4Xjwd4vX7tJM10116	rogid:ogsHtvyxaAmmFUflijf6YfBuW7U10116	intact-crc:5739064715290C02|rigid:X2pWxKyymRGNVBG4TspcQwsM54M	false	his tag:?-?	ha tag:?-?	-	-	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)
uniprotkb:P51579	uniprotkb:P51577	intact:EBI-9511515|uniprotkb:R9PXR5|ensembl:ENSRNOP00000002558	intact:EBI-9511617|ensembl:ENSRNOP00000001752	psi-mi:p2rx6_rat(display_long)|uniprotkb:P2rx6(gene name)|psi-mi:P2rx6(display_short)|uniprotkb:P2rxl1(gene name synonym)|uniprotkb:ATP receptor(gene name synonym)|uniprotkb:P2XM(gene name synonym)|uniprotkb:Purinergic receptor(gene name synonym)|uniprotkb:Purinergic receptor P2X-like 1(gene name synonym)	psi-mi:p2rx4_rat(display_long)|uniprotkb:P2rx4(gene name)|psi-mi:P2rx4(display_short)|uniprotkb:ATP receptor(gene name synonym)|uniprotkb:Purinergic receptor(gene name synonym)	psi-mi:"MI:0007"(anti tag coimmunoprecipitation)	Antonio et al. (2014)	pubmed:24815693|imex:IM-22591	taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)")	taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)")	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-9511742|imex:IM-22591-7	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	rgd:3243|refseq:NP_036853.2|ensembl:ENSRNOG00000001873(gene)|ensembl:ENSRNOT00000002558(transcript)|go:"GO:0001614"(purinergic nucleotide receptor activity)|go:"GO:0004931"(extracellularly ATP-gated cation channel activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005639"(integral component of nuclear inner membrane)|go:"GO:0005737"(cytoplasm)|go:"GO:0014069"(postsynaptic density)|go:"GO:0016020"(membrane)|go:"GO:0030054"(cell junction)|go:"GO:0033198"(response to ATP)|go:"GO:0043025"(neuronal cell body)|go:"GO:0043197"(dendritic spine)|go:"GO:0043235"(receptor complex)|go:"GO:0044877"(protein-containing complex binding)|go:"GO:0098688"(parallel fiber to Purkinje cell synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099060"(integral component of postsynaptic specialization membrane)|interpro:IPR001429(P2X purinoreceptor)|interpro:IPR003049(P2X6 purinoceptor)|interpro:IPR027309|reactome:R-RNO-139853|reactome:R-RNO-418346	rgd:62073|refseq:NP_113782.1|dip:DIP-48411N|ensembl:ENSRNOG00000001300(gene)|ensembl:ENSRNOT00000001752(transcript)|go:"GO:0001614"(purinergic nucleotide receptor activity)|go:"GO:0002028"(regulation of sodium ion transport)|go:"GO:0002931"(response to ischemia)|go:"GO:0004931"(extracellularly ATP-gated cation channel activity)|go:"GO:0005102"(signaling receptor binding)|go:"GO:0005507"(copper ion binding)|go:"GO:0005524"(ATP binding)|go:"GO:0005639"(integral component of nuclear inner membrane)|go:"GO:0005886"(plasma membrane)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0006809"(nitric oxide biosynthetic process)|go:"GO:0006816"(calcium ion transport)|go:"GO:0007165"(signal transduction)|go:"GO:0008217"(regulation of blood pressure)|go:"GO:0008270"(zinc ion binding)|go:"GO:0010524"(positive regulation of calcium ion transport into cytosol)|go:"GO:0010614"(negative regulation of cardiac muscle hypertrophy)|go:"GO:0014069"(postsynaptic density)|go:"GO:0016020"(membrane)|go:"GO:0019228"(neuronal action potential)|go:"GO:0019233"(sensory perception of pain)|go:"GO:0030054"(cell junction)|go:"GO:0030424"(axon)|go:"GO:0030425"(dendrite)|go:"GO:0033198"(response to ATP)|go:"GO:0034220"(ion transmembrane transport)|go:"GO:0034405"(response to fluid shear stress)|go:"GO:0035590"(purinergic nucleotide receptor signaling pathway)|go:"GO:0042311"(vasodilation)|go:"GO:0042802"(identical protein binding)|go:"GO:0043025"(neuronal cell body)|go:"GO:0043195"(terminal bouton)|go:"GO:0043197"(dendritic spine)|go:"GO:0043536"(positive regulation of blood vessel endothelial cell migration)|go:"GO:0044297"(cell body)|go:"GO:0045177"(apical part of cell)|go:"GO:0045202"(synapse)|go:"GO:0045296"(cadherin binding)|go:"GO:0048266"(behavioral response to pain)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0048678"(response to axon injury)|go:"GO:0050850"(positive regulation of calcium-mediated signaling)|go:"GO:0050920"(regulation of chemotaxis)|go:"GO:0050975"(sensory perception of touch)|go:"GO:0051897"(positive regulation of protein kinase B signaling)|go:"GO:0051899"(membrane depolarization)|go:"GO:0055117"(regulation of cardiac muscle contraction)|go:"GO:0055119"(relaxation of cardiac muscle)|go:"GO:0060079"(excitatory postsynaptic potential)|go:"GO:0070588"(calcium ion transmembrane transport)|go:"GO:0071294"(cellular response to zinc ion)|go:"GO:0071318"(cellular response to ATP)|go:"GO:0097190"(apoptotic signaling pathway)|go:"GO:0098662"(inorganic cation transmembrane transport)|go:"GO:0099604"(ligand-gated calcium channel activity)|go:"GO:1904141"(positive regulation of microglial cell migration)|go:"GO:2001028"(positive regulation of endothelial cell chemotaxis)|interpro:IPR001429(P2X purinoreceptor)|interpro:IPR003047(P2X4 purinoceptor)|interpro:IPR027309|rcsb pdb:2BP5|rcsb pdb:2RUP|reactome:R-RNO-139853|reactome:R-RNO-418346	-	-	-	figure legend:f2|comment:"A detergent extract of cells co-expressing His6-P2X2, P2X4-HA and FLAG-P2X6 was  subjected to sequential anti-HA and anti-FLAG immunoaffinity chromatography steps (Fig.  2). The first (anti-HA) step would be expected capture any protein complex containing P2X4-  HA, while the second (anti-FLAG) step would capture complexes containing both P2X4-HA  and FLAG-P2X6. The immunoblots showed that this was indeed the case, and also indicated  that His6-P2X2 was also present in the second eluate. The same result was obtained when the  8  order of the immunoaffinity chromatography steps was reversed. Fig. 2 also shows that when  either FLAG-P2X6 or P2X4-HA was omitted, none of the three subunits appeared in the  second eluate. These results indicate an intimate association between P2X2, P2X4 and P2X6."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:9606(human-tsa201)|taxid:9606(Homo sapiens subclone of human embryonic kidney 293 cell line that expresses simian virus 40 T antigen)	-	2014/04/28	2018/02/26	rogid:cTu+Raxv9pYr4tIXWDZiTfNHMl410116	rogid:ogsHtvyxaAmmFUflijf6YfBuW7U10116	intact-crc:B35ADBD1CABEFC8C|rigid:xof/JpuCYhg9LAbOLA4Soop2ZMU	false	flag tag:?-?	ha tag:?-?	-	-	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)
uniprotkb:P51579	uniprotkb:P49653	intact:EBI-9511515|uniprotkb:R9PXR5|ensembl:ENSRNOP00000002558	intact:EBI-9511543|uniprotkb:O54868	psi-mi:p2rx6_rat(display_long)|uniprotkb:P2rx6(gene name)|psi-mi:P2rx6(display_short)|uniprotkb:P2rxl1(gene name synonym)|uniprotkb:ATP receptor(gene name synonym)|uniprotkb:P2XM(gene name synonym)|uniprotkb:Purinergic receptor(gene name synonym)|uniprotkb:Purinergic receptor P2X-like 1(gene name synonym)	psi-mi:p2rx2_rat(display_long)|uniprotkb:P2rx2(gene name)|psi-mi:P2rx2(display_short)|uniprotkb:ATP receptor(gene name synonym)|uniprotkb:Purinergic receptor(gene name synonym)	psi-mi:"MI:0007"(anti tag coimmunoprecipitation)	Antonio et al. (2014)	pubmed:24815693|imex:IM-22591	taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)")	taxid:10116(rat)|taxid:10116("Rattus norvegicus (Rat)")	psi-mi:"MI:0914"(association)	psi-mi:"MI:0471"(MINT)	intact:EBI-9511742|imex:IM-22591-7	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	rgd:3243|refseq:NP_036853.2|ensembl:ENSRNOG00000001873(gene)|ensembl:ENSRNOT00000002558(transcript)|go:"GO:0001614"(purinergic nucleotide receptor activity)|go:"GO:0004931"(extracellularly ATP-gated cation channel activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005639"(integral component of nuclear inner membrane)|go:"GO:0005737"(cytoplasm)|go:"GO:0014069"(postsynaptic density)|go:"GO:0016020"(membrane)|go:"GO:0030054"(cell junction)|go:"GO:0033198"(response to ATP)|go:"GO:0043025"(neuronal cell body)|go:"GO:0043197"(dendritic spine)|go:"GO:0043235"(receptor complex)|go:"GO:0044877"(protein-containing complex binding)|go:"GO:0098688"(parallel fiber to Purkinje cell synapse)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099060"(integral component of postsynaptic specialization membrane)|interpro:IPR001429(P2X purinoreceptor)|interpro:IPR003049(P2X6 purinoceptor)|interpro:IPR027309|reactome:R-RNO-139853|reactome:R-RNO-418346	reactome:R-RNO-139853|reactome:R-RNO-418346|rgd:620251|refseq:NP_446108.2|dip:DIP-48329N|go:"GO:0001614"(purinergic nucleotide receptor activity)|go:"GO:0001666"(response to hypoxia)|go:"GO:0002931"(response to ischemia)|go:"GO:0003029"(detection of hypoxic conditions in blood by carotid body chemoreceptor signaling)|go:"GO:0004931"(extracellularly ATP-gated cation channel activity)|go:"GO:0005507"(copper ion binding)|go:"GO:0005524"(ATP binding)|go:"GO:0005639"(integral component of nuclear inner membrane)|go:"GO:0005886"(plasma membrane)|go:"GO:0005887"(integral component of plasma membrane)|go:"GO:0007268"(chemical synaptic transmission)|go:"GO:0007274"(neuromuscular synaptic transmission)|go:"GO:0007528"(neuromuscular junction development)|go:"GO:0007605"(sensory perception of sound)|go:"GO:0008270"(zinc ion binding)|go:"GO:0009743"(response to carbohydrate)|go:"GO:0009986"(cell surface)|go:"GO:0010033"(response to organic substance)|go:"GO:0014069"(postsynaptic density)|go:"GO:0014832"(urinary bladder smooth muscle contraction)|go:"GO:0015276"(ligand-gated ion channel activity)|go:"GO:0016020"(membrane)|go:"GO:0016021"(integral component of membrane)|go:"GO:0016151"(nickel cation binding)|go:"GO:0016324"(apical plasma membrane)|go:"GO:0019228"(neuronal action potential)|go:"GO:0030432"(peristalsis)|go:"GO:0033198"(response to ATP)|go:"GO:0035091"(phosphatidylinositol binding)|go:"GO:0042734"(presynaptic membrane)|go:"GO:0042802"(identical protein binding)|go:"GO:0043025"(neuronal cell body)|go:"GO:0043195"(terminal bouton)|go:"GO:0043197"(dendritic spine)|go:"GO:0043235"(receptor complex)|go:"GO:0044877"(protein-containing complex binding)|go:"GO:0045340"(mercury ion binding)|go:"GO:0046870"(cadmium ion binding)|go:"GO:0048266"(behavioral response to pain)|go:"GO:0048741"(skeletal muscle fiber development)|go:"GO:0050897"(cobalt ion binding)|go:"GO:0050909"(sensory perception of taste)|go:"GO:0098978"(glutamatergic synapse)|go:"GO:0099059"(integral component of presynaptic active zone membrane)|go:"GO:0099509"(regulation of presynaptic cytosolic calcium ion concentration)|go:"GO:2000300"(regulation of synaptic vesicle exocytosis)|interpro:IPR001429(P2X purinoreceptor)|interpro:IPR003045(P2X2 purinoceptor)|interpro:IPR027309	-	-	-	figure legend:f2|comment:"A detergent extract of cells co-expressing His6-P2X2, P2X4-HA and FLAG-P2X6 was  subjected to sequential anti-HA and anti-FLAG immunoaffinity chromatography steps (Fig.  2). The first (anti-HA) step would be expected capture any protein complex containing P2X4-  HA, while the second (anti-FLAG) step would capture complexes containing both P2X4-HA  and FLAG-P2X6. The immunoblots showed that this was indeed the case, and also indicated  that His6-P2X2 was also present in the second eluate. The same result was obtained when the  8  order of the immunoaffinity chromatography steps was reversed. Fig. 2 also shows that when  either FLAG-P2X6 or P2X4-HA was omitted, none of the three subunits appeared in the  second eluate. These results indicate an intimate association between P2X2, P2X4 and P2X6."|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:9606(human-tsa201)|taxid:9606(Homo sapiens subclone of human embryonic kidney 293 cell line that expresses simian virus 40 T antigen)	-	2014/04/28	2018/02/26	rogid:cTu+Raxv9pYr4tIXWDZiTfNHMl410116	rogid:rHLp0w8RIFBzocD4Xjwd4vX7tJM10116	intact-crc:B35ADBD1CABEFC8C|rigid:xof/JpuCYhg9LAbOLA4Soop2ZMU	false	flag tag:?-?	his tag:?-?	-	-	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)