#ID(s) interactor A	ID(s) interactor B	Alt. ID(s) interactor A	Alt. ID(s) interactor B	Alias(es) interactor A	Alias(es) interactor B	Interaction detection method(s)	Publication 1st author(s)	Publication Identifier(s)	Taxid interactor A	Taxid interactor B	Interaction type(s)	Source database(s)	Interaction identifier(s)	Confidence value(s)	Expansion method(s)	Biological role(s) interactor A	Biological role(s) interactor B	Experimental role(s) interactor A	Experimental role(s) interactor B	Type(s) interactor A	Type(s) interactor B	Xref(s) interactor A	Xref(s) interactor B	Interaction Xref(s)	Annotation(s) interactor A	Annotation(s) interactor B	Interaction annotation(s)	Host organism(s)	Interaction parameter(s)	Creation date	Update date	Checksum(s) interactor A	Checksum(s) interactor B	Interaction Checksum(s)	Negative	Feature(s) interactor A	Feature(s) interactor B	Stoichiometry(s) interactor A	Stoichiometry(s) interactor B	Identification method participant A	Identification method participant B
uniprotkb:Q02750	uniprotkb:P10398	intact:EBI-492564|ensembl:ENSP00000302486	intact:EBI-365961|uniprotkb:P07557|uniprotkb:Q5H9B3|intact:EBI-28988863|ensembl:ENSP00000366244|uniprotkb:Q5H9B2	psi-mi:mp2k1_human(display_long)|uniprotkb:ERK activator kinase 1(gene name synonym)|uniprotkb:MAPK/ERK kinase 1(gene name synonym)|uniprotkb:MAP2K1(gene name)|psi-mi:MAP2K1(display_short)|uniprotkb:MEK1(gene name synonym)|uniprotkb:PRKMK1(gene name synonym)	psi-mi:araf_human(display_long)|uniprotkb:ARAF(gene name)|psi-mi:ARAF(display_short)|uniprotkb:PKS(gene name synonym)|uniprotkb:PKS2(gene name synonym)|uniprotkb:ARAF1(gene name synonym)|uniprotkb:Proto-oncogene A-Raf-1(gene name synonym)|uniprotkb:Proto-oncogene Pks(gene name synonym)|uniprotkb:Proto-oncogene A-Raf(gene name synonym)	psi-mi:"MI:0007"(anti tag coimmunoprecipitation)	Lito et al. (2014)	pubmed:24746704|imex:IM-22983	taxid:9606(human)|taxid:9606(Homo sapiens)	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-9549253|imex:IM-22983-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_002746.1|refseq:XP_016877900.1|dip:DIP-201N|ensembl:ENSG00000169032(gene)|ensembl:ENST00000307102(transcript)|go:"GO:0000187"(obsolete activation of MAPK activity)|go:"GO:0004672"(protein kinase activity)|go:"GO:0004674"(protein serine/threonine kinase activity)|go:"GO:0004708"(MAP kinase kinase activity)|go:"GO:0004712"(protein serine/threonine/tyrosine kinase activity)|go:"GO:0004714"(transmembrane receptor protein tyrosine kinase activity)|go:"GO:0005078"(MAP-kinase scaffold activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005634"(nucleus)|go:"GO:0005739"(mitochondrion)|go:"GO:0005769"(early endosome)|go:"GO:0005770"(late endosome)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005815"(microtubule organizing center)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0005925"(focal adhesion)|go:"GO:0006468"(protein phosphorylation)|go:"GO:0006935"(chemotaxis)|go:"GO:0007165"(signal transduction)|go:"GO:0007507"(heart development)|go:"GO:0008022"(protein C-terminus binding)|go:"GO:0008285"(negative regulation of cell population proliferation)|go:"GO:0010628"(positive regulation of gene expression)|go:"GO:0018107"(peptidyl-threonine phosphorylation)|go:"GO:0021697"(cerebellar cortex formation)|go:"GO:0030182"(neuron differentiation)|go:"GO:0030216"(keratinocyte differentiation)|go:"GO:0030878"(thyroid gland development)|go:"GO:0032872"(regulation of stress-activated MAPK cascade)|go:"GO:0043539"(protein serine/threonine kinase activator activity)|go:"GO:0045893"(positive regulation of transcription, DNA-templated)|go:"GO:0047485"(protein N-terminus binding)|go:"GO:0048538"(thymus development)|go:"GO:0048679"(regulation of axon regeneration)|go:"GO:0048870"(cell motility)|go:"GO:0050772"(positive regulation of axonogenesis)|go:"GO:0051726"(regulation of cell cycle)|go:"GO:0060020"(Bergmann glial cell differentiation)|go:"GO:0060324"(face development)|go:"GO:0060440"(trachea formation)|go:"GO:0060502"(epithelial cell proliferation involved in lung morphogenesis)|go:"GO:0060674"(placenta blood vessel development)|go:"GO:0060711"(labyrinthine layer development)|go:"GO:0070371"(ERK1 and ERK2 cascade)|go:"GO:0070374"(positive regulation of ERK1 and ERK2 cascade)|go:"GO:0071902"(positive regulation of protein serine/threonine kinase activity)|go:"GO:0090170"(regulation of Golgi inheritance)|go:"GO:0090398"(cellular senescence)|go:"GO:0097110"(scaffold protein binding)|go:"GO:0106310"(protein serine kinase activity)|go:"GO:0106311"|go:"GO:1903800"(positive regulation of production of miRNAs involved in gene silencing by miRNA)|go:"GO:2000641"(regulation of early endosome to late endosome transport)|interpro:IPR000719(Protein kinase, core)|interpro:IPR008271(Serine/threonine protein kinase, active site)|interpro:IPR011009(Protein kinase-like)|interpro:IPR017441(Protein kinase ATP binding, conserved site)|mint:Q02750|rcsb pdb:1S9J|rcsb pdb:2P55|rcsb pdb:3DV3|rcsb pdb:3DY7|rcsb pdb:3E8N|rcsb pdb:3EQB|rcsb pdb:3EQC|rcsb pdb:3EQD|rcsb pdb:3EQF|rcsb pdb:3EQG|rcsb pdb:3EQH|rcsb pdb:3EQI|rcsb pdb:3MBL|rcsb pdb:3ORN|rcsb pdb:3OS3|rcsb pdb:3PP1|rcsb pdb:3SLS|rcsb pdb:3V01|rcsb pdb:3V04|rcsb pdb:3VVH|rcsb pdb:3W8Q|rcsb pdb:3WIG|rcsb pdb:3ZLS|rcsb pdb:3ZLW|rcsb pdb:3ZLX|rcsb pdb:3ZLY|rcsb pdb:3ZM4|rcsb pdb:4AN2|rcsb pdb:4AN3|rcsb pdb:4AN9|rcsb pdb:4ANB|rcsb pdb:4ARK|rcsb pdb:4LMN|rcsb pdb:4MNE|rcsb pdb:4U7Z|rcsb pdb:4U80|rcsb pdb:4U81|rcsb pdb:5EYM|rcsb pdb:5HZE|rcsb pdb:5YT3|rcsb pdb:6NYB|rcsb pdb:6PP9|rcsb pdb:6Q0J|rcsb pdb:6Q0T|rcsb pdb:6U2G|rcsb pdb:6V2W|rcsb pdb:6X2P|rcsb pdb:6X2S|rcsb pdb:6X2X|rcsb pdb:7B3M|rcsb pdb:7B7R|rcsb pdb:7B94|rcsb pdb:7B9L|rcsb pdb:7M0T|rcsb pdb:7M0U|rcsb pdb:7M0V|rcsb pdb:7M0W|rcsb pdb:7M0X|rcsb pdb:7M0Y|rcsb pdb:7M0Z|reactome:R-HSA-110056|reactome:R-HSA-170968|reactome:R-HSA-445144|reactome:R-HSA-5210891|reactome:R-HSA-5673000|reactome:R-HSA-5674135|reactome:R-HSA-5674499|reactome:R-HSA-5684264|reactome:R-HSA-6802946|reactome:R-HSA-6802948|reactome:R-HSA-6802952|reactome:R-HSA-6802955|reactome:R-HSA-9649948|reactome:R-HSA-9652169|reactome:R-HSA-9656223|rcsb pdb:5BX0	ensembl:ENSG00000078061(gene)|ensembl:ENST00000377045(transcript)|go:"GO:0004672"(protein kinase activity)|go:"GO:0004674"(protein serine/threonine kinase activity)|go:"GO:0004709"(MAP kinase kinase kinase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005739"(mitochondrion)|go:"GO:0005829"(cytosol)|go:"GO:0006464"(cellular protein modification process)|go:"GO:0032006"(regulation of TOR signaling)|go:"GO:0032434"(regulation of proteasomal ubiquitin-dependent protein catabolic process)|go:"GO:0033138"(positive regulation of peptidyl-serine phosphorylation)|go:"GO:0043066"(negative regulation of apoptotic process)|go:"GO:0046872"(metal ion binding)|go:"GO:0106310"(protein serine kinase activity)|go:"GO:0106311"|interpro:IPR000719(Protein kinase, core)|interpro:IPR001245(Tyrosine protein kinase)|interpro:IPR002219(Protein kinase C, phorbol ester/diacylglycerol binding)|interpro:IPR003116(Raf-like Ras-binding)|interpro:IPR008271(Serine/threonine protein kinase, active site)|interpro:IPR011009(Protein kinase-like)|interpro:IPR017441(Protein kinase ATP binding, conserved site)|interpro:IPR020454|interpro:IPR029071|mint:P10398|rcsb pdb:2MSE|reactome:R-HSA-5673000|reactome:R-HSA-5674135|reactome:R-HSA-5675221|reactome:R-HSA-6802946|reactome:R-HSA-6802948|reactome:R-HSA-6802952|reactome:R-HSA-6802955|reactome:R-HSA-9649948|reactome:R-HSA-9656223|reactome:R-HSA-9660537|rcsb pdb:1WXM|refseq:NP_001645.1|refseq:XP_011542210.1|refseq:NP_001243125.1|ensembl:ENST00000377039|ensembl:ENSP00000366238|ensembl:ENSP00000366244|refseq:NP_001243126.1|dip:DIP-31374N	-	-	-	figure legend:4 A/D, 6A, 7G, Supp  figure 4 A|agonist:"MEK inhibitors: Selumetinib and PD0325901  CH5126766 - a newer MEK inhibitor."|antagonist:Trametinib -a newer MEK inhibitor, disrupts the interaction of MEK1 with ARAF and CRAF.|comment:In cells co-transfected with K-RAS G12V   this interaction was completely disrupted, but was only partially reduced in the presence of CH5126766 or PD0325901.  Co-transfection of BRAF V600E reduced this interaction,and  the presence of CH5126766 or PD0325901 further reduced the interaction , but in the presence of CH5126766 the interaction was increased.|dataset:Cancer - Interactions investigated in the context of cancer|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:9606(human-293)|taxid:9606(Homo sapiens transformed primary embryonal kidney cells)	-	2014/06/12	2015/05/15	rogid:ieBLcDINn9hZFZZEIQ3Ktdf2NF89606	rogid:WRtbdrEuDxNkA6jRRxvapodFcUE9606	intact-crc:695C8F4CF92B4292|rigid:VLl+5T+aNh8kZKnWh1WwvnrN0PI	false	flag tag:?-?|mutation decreasing interaction:218-218,222-222|mutation disrupting interaction:308-308,310-310	-	-	-	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)
uniprotkb:Q02750	uniprotkb:P04049	intact:EBI-492564|ensembl:ENSP00000302486	intact:EBI-365996|uniprotkb:B0LPH8|uniprotkb:Q15278|uniprotkb:Q9UC20|ensembl:ENSP00000251849|uniprotkb:B2R5N3	psi-mi:mp2k1_human(display_long)|uniprotkb:ERK activator kinase 1(gene name synonym)|uniprotkb:MAPK/ERK kinase 1(gene name synonym)|uniprotkb:MAP2K1(gene name)|psi-mi:MAP2K1(display_short)|uniprotkb:MEK1(gene name synonym)|uniprotkb:PRKMK1(gene name synonym)	psi-mi:raf1_human(display_long)|uniprotkb:RAF1(gene name)|psi-mi:RAF1(display_short)|uniprotkb:RAF(gene name synonym)|uniprotkb:Proto-oncogene c-RAF(gene name synonym)|uniprotkb:Raf-1(gene name synonym)	psi-mi:"MI:0007"(anti tag coimmunoprecipitation)	Lito et al. (2014)	pubmed:24746704|imex:IM-22983	taxid:9606(human)|taxid:9606(Homo sapiens)	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-9549253|imex:IM-22983-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_002746.1|refseq:XP_016877900.1|dip:DIP-201N|ensembl:ENSG00000169032(gene)|ensembl:ENST00000307102(transcript)|go:"GO:0000187"(obsolete activation of MAPK activity)|go:"GO:0004672"(protein kinase activity)|go:"GO:0004674"(protein serine/threonine kinase activity)|go:"GO:0004708"(MAP kinase kinase activity)|go:"GO:0004712"(protein serine/threonine/tyrosine kinase activity)|go:"GO:0004714"(transmembrane receptor protein tyrosine kinase activity)|go:"GO:0005078"(MAP-kinase scaffold activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005634"(nucleus)|go:"GO:0005739"(mitochondrion)|go:"GO:0005769"(early endosome)|go:"GO:0005770"(late endosome)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005815"(microtubule organizing center)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0005925"(focal adhesion)|go:"GO:0006468"(protein phosphorylation)|go:"GO:0006935"(chemotaxis)|go:"GO:0007165"(signal transduction)|go:"GO:0007507"(heart development)|go:"GO:0008022"(protein C-terminus binding)|go:"GO:0008285"(negative regulation of cell population proliferation)|go:"GO:0010628"(positive regulation of gene expression)|go:"GO:0018107"(peptidyl-threonine phosphorylation)|go:"GO:0021697"(cerebellar cortex formation)|go:"GO:0030182"(neuron differentiation)|go:"GO:0030216"(keratinocyte differentiation)|go:"GO:0030878"(thyroid gland development)|go:"GO:0032872"(regulation of stress-activated MAPK cascade)|go:"GO:0043539"(protein serine/threonine kinase activator activity)|go:"GO:0045893"(positive regulation of transcription, DNA-templated)|go:"GO:0047485"(protein N-terminus binding)|go:"GO:0048538"(thymus development)|go:"GO:0048679"(regulation of axon regeneration)|go:"GO:0048870"(cell motility)|go:"GO:0050772"(positive regulation of axonogenesis)|go:"GO:0051726"(regulation of cell cycle)|go:"GO:0060020"(Bergmann glial cell differentiation)|go:"GO:0060324"(face development)|go:"GO:0060440"(trachea formation)|go:"GO:0060502"(epithelial cell proliferation involved in lung morphogenesis)|go:"GO:0060674"(placenta blood vessel development)|go:"GO:0060711"(labyrinthine layer development)|go:"GO:0070371"(ERK1 and ERK2 cascade)|go:"GO:0070374"(positive regulation of ERK1 and ERK2 cascade)|go:"GO:0071902"(positive regulation of protein serine/threonine kinase activity)|go:"GO:0090170"(regulation of Golgi inheritance)|go:"GO:0090398"(cellular senescence)|go:"GO:0097110"(scaffold protein binding)|go:"GO:0106310"(protein serine kinase activity)|go:"GO:0106311"|go:"GO:1903800"(positive regulation of production of miRNAs involved in gene silencing by miRNA)|go:"GO:2000641"(regulation of early endosome to late endosome transport)|interpro:IPR000719(Protein kinase, core)|interpro:IPR008271(Serine/threonine protein kinase, active site)|interpro:IPR011009(Protein kinase-like)|interpro:IPR017441(Protein kinase ATP binding, conserved site)|mint:Q02750|rcsb pdb:1S9J|rcsb pdb:2P55|rcsb pdb:3DV3|rcsb pdb:3DY7|rcsb pdb:3E8N|rcsb pdb:3EQB|rcsb pdb:3EQC|rcsb pdb:3EQD|rcsb pdb:3EQF|rcsb pdb:3EQG|rcsb pdb:3EQH|rcsb pdb:3EQI|rcsb pdb:3MBL|rcsb pdb:3ORN|rcsb pdb:3OS3|rcsb pdb:3PP1|rcsb pdb:3SLS|rcsb pdb:3V01|rcsb pdb:3V04|rcsb pdb:3VVH|rcsb pdb:3W8Q|rcsb pdb:3WIG|rcsb pdb:3ZLS|rcsb pdb:3ZLW|rcsb pdb:3ZLX|rcsb pdb:3ZLY|rcsb pdb:3ZM4|rcsb pdb:4AN2|rcsb pdb:4AN3|rcsb pdb:4AN9|rcsb pdb:4ANB|rcsb pdb:4ARK|rcsb pdb:4LMN|rcsb pdb:4MNE|rcsb pdb:4U7Z|rcsb pdb:4U80|rcsb pdb:4U81|rcsb pdb:5EYM|rcsb pdb:5HZE|rcsb pdb:5YT3|rcsb pdb:6NYB|rcsb pdb:6PP9|rcsb pdb:6Q0J|rcsb pdb:6Q0T|rcsb pdb:6U2G|rcsb pdb:6V2W|rcsb pdb:6X2P|rcsb pdb:6X2S|rcsb pdb:6X2X|rcsb pdb:7B3M|rcsb pdb:7B7R|rcsb pdb:7B94|rcsb pdb:7B9L|rcsb pdb:7M0T|rcsb pdb:7M0U|rcsb pdb:7M0V|rcsb pdb:7M0W|rcsb pdb:7M0X|rcsb pdb:7M0Y|rcsb pdb:7M0Z|reactome:R-HSA-110056|reactome:R-HSA-170968|reactome:R-HSA-445144|reactome:R-HSA-5210891|reactome:R-HSA-5673000|reactome:R-HSA-5674135|reactome:R-HSA-5674499|reactome:R-HSA-5684264|reactome:R-HSA-6802946|reactome:R-HSA-6802948|reactome:R-HSA-6802952|reactome:R-HSA-6802955|reactome:R-HSA-9649948|reactome:R-HSA-9652169|reactome:R-HSA-9656223|rcsb pdb:5BX0	rcsb pdb:3IQV|rcsb pdb:3KUC|rcsb pdb:3KUD|rcsb pdb:3NKX|rcsb pdb:3O8I|rcsb pdb:3OMV|rcsb pdb:4FJ3|rcsb pdb:4G0N|rcsb pdb:4G3X|rcsb pdb:4IEA|rcsb pdb:4IHL|rcsb pdb:6NTC|rcsb pdb:6NTD|rcsb pdb:6PTS|rcsb pdb:6PTW|rcsb pdb:6VJJ|rcsb pdb:6XGU|rcsb pdb:6XGV|rcsb pdb:6XHA|rcsb pdb:6XHB|rcsb pdb:6XI7|ensembl:ENSG00000132155(gene)|ensembl:ENST00000251849(transcript)|go:"GO:0000186"(obsolete activation of MAPKK activity)|go:"GO:0001666"(response to hypoxia)|go:"GO:0004672"(protein kinase activity)|go:"GO:0004674"(protein serine/threonine kinase activity)|go:"GO:0004709"(MAP kinase kinase kinase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005737"(cytoplasm)|go:"GO:0005739"(mitochondrion)|go:"GO:0005741"(mitochondrial outer membrane)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0006468"(protein phosphorylation)|go:"GO:0006915"(apoptotic process)|go:"GO:0007165"(signal transduction)|go:"GO:0007190"(activation of adenylate cyclase activity)|go:"GO:0007507"(heart development)|go:"GO:0008179"(adenylate cyclase binding)|go:"GO:0008285"(negative regulation of cell population proliferation)|go:"GO:0010856"(adenylate cyclase activator activity)|go:"GO:0016607"(nuclear speck)|go:"GO:0019899"(enzyme binding)|go:"GO:0030154"(cell differentiation)|go:"GO:0030878"(thyroid gland development)|go:"GO:0031143"(pseudopodium)|go:"GO:0031333"(negative regulation of protein-containing complex assembly)|go:"GO:0031434"(mitogen-activated protein kinase kinase binding)|go:"GO:0033138"(positive regulation of peptidyl-serine phosphorylation)|go:"GO:0035019"(somatic stem cell population maintenance)|go:"GO:0035023"(regulation of Rho protein signal transduction)|go:"GO:0035773"(insulin secretion involved in cellular response to glucose stimulus)|go:"GO:0035994"(response to muscle stretch)|go:"GO:0042060"(wound healing)|go:"GO:0042802"(identical protein binding)|go:"GO:0042981"(regulation of apoptotic process)|go:"GO:0043066"(negative regulation of apoptotic process)|go:"GO:0043154"(negative regulation of cysteine-type endopeptidase activity involved in apoptotic process)|go:"GO:0044877"(protein-containing complex binding)|go:"GO:0045104"(intermediate filament cytoskeleton organization)|go:"GO:0045595"(regulation of cell differentiation)|go:"GO:0045944"(positive regulation of transcription by RNA polymerase II)|go:"GO:0046872"(metal ion binding)|go:"GO:0048011"(neurotrophin TRK receptor signaling pathway)|go:"GO:0048538"(thymus development)|go:"GO:0060324"(face development)|go:"GO:0071550"(death-inducing signaling complex assembly)|go:"GO:0106310"(protein serine kinase activity)|go:"GO:0106311"|go:"GO:1902042"(negative regulation of extrinsic apoptotic signaling pathway via death domain receptors)|go:"GO:2000145"(regulation of cell motility)|interpro:IPR000719(Protein kinase, core)|interpro:IPR001245(Tyrosine protein kinase)|interpro:IPR002219(Protein kinase C, phorbol ester/diacylglycerol binding)|interpro:IPR003116(Raf-like Ras-binding)|interpro:IPR008271(Serine/threonine protein kinase, active site)|interpro:IPR011009(Protein kinase-like)|interpro:IPR017441(Protein kinase ATP binding, conserved site)|interpro:IPR020454|interpro:IPR029071|mint:P04049|rcsb pdb:1C1Y|rcsb pdb:1FAQ|rcsb pdb:1FAR|rcsb pdb:1GUA|rcsb pdb:1RFA|rcsb pdb:3CU8|rcsb pdb:3IQJ|rcsb pdb:3IQU|rcsb pdb:7JHP|reactome:R-HSA-2672351|reactome:R-HSA-392517|reactome:R-HSA-430116|reactome:R-HSA-5621575|reactome:R-HSA-5673000|reactome:R-HSA-5674135|reactome:R-HSA-5674499|reactome:R-HSA-5675221|reactome:R-HSA-6802946|reactome:R-HSA-6802948|reactome:R-HSA-6802952|reactome:R-HSA-6802955|reactome:R-HSA-9649948|reactome:R-HSA-9656223|reactome:R-HSA-9660537|refseq:NP_002871.1|refseq:XP_011532276.1|refseq:XP_005265412.1|dip:DIP-1048N	-	-	-	figure legend:4 A/D, 6A, 7G, Supp  figure 4 A|agonist:"MEK inhibitors: Selumetinib and PD0325901  CH5126766 - a newer MEK inhibitor."|antagonist:Trametinib -a newer MEK inhibitor, disrupts the interaction of MEK1 with ARAF and CRAF.|comment:In cells co-transfected with K-RAS G12V   this interaction was completely disrupted, but was only partially reduced in the presence of CH5126766 or PD0325901.  Co-transfection of BRAF V600E reduced this interaction,and  the presence of CH5126766 or PD0325901 further reduced the interaction , but in the presence of CH5126766 the interaction was increased.|dataset:Cancer - Interactions investigated in the context of cancer|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:9606(human-293)|taxid:9606(Homo sapiens transformed primary embryonal kidney cells)	-	2014/06/12	2015/05/15	rogid:ieBLcDINn9hZFZZEIQ3Ktdf2NF89606	rogid:1WOAPuzcZdMoq9hyFD/qr4Tz7049606	intact-crc:695C8F4CF92B4292|rigid:VLl+5T+aNh8kZKnWh1WwvnrN0PI	false	flag tag:?-?|mutation decreasing interaction:218-218,222-222|mutation disrupting interaction:308-308,310-310	-	-	-	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)
uniprotkb:Q02750	uniprotkb:P15056	intact:EBI-492564|ensembl:ENSP00000302486	intact:EBI-365980|uniprotkb:Q13878|uniprotkb:Q3MIN6|intact:EBI-28988924|ensembl:ENSP00000493543|uniprotkb:Q9UDP8|uniprotkb:Q9Y6T3|uniprotkb:A4D1T4|uniprotkb:B6HY61|uniprotkb:B6HY62|uniprotkb:B6HY63|uniprotkb:B6HY64|uniprotkb:B6HY65|uniprotkb:B6HY66	psi-mi:mp2k1_human(display_long)|uniprotkb:ERK activator kinase 1(gene name synonym)|uniprotkb:MAPK/ERK kinase 1(gene name synonym)|uniprotkb:MAP2K1(gene name)|psi-mi:MAP2K1(display_short)|uniprotkb:MEK1(gene name synonym)|uniprotkb:PRKMK1(gene name synonym)	psi-mi:braf_human(display_long)|uniprotkb:BRAF(gene name)|psi-mi:BRAF(display_short)|uniprotkb:BRAF1(gene name synonym)|uniprotkb:RAFB1(gene name synonym)|uniprotkb:v-Raf murine sarcoma viral oncogene homolog B1(gene name synonym)|uniprotkb:p94(gene name synonym)|uniprotkb:Proto-oncogene B-Raf(gene name synonym)	psi-mi:"MI:0007"(anti tag coimmunoprecipitation)	Lito et al. (2014)	pubmed:24746704|imex:IM-22983	taxid:9606(human)|taxid:9606(Homo sapiens)	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-9549253|imex:IM-22983-1	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_002746.1|refseq:XP_016877900.1|dip:DIP-201N|ensembl:ENSG00000169032(gene)|ensembl:ENST00000307102(transcript)|go:"GO:0000187"(obsolete activation of MAPK activity)|go:"GO:0004672"(protein kinase activity)|go:"GO:0004674"(protein serine/threonine kinase activity)|go:"GO:0004708"(MAP kinase kinase activity)|go:"GO:0004712"(protein serine/threonine/tyrosine kinase activity)|go:"GO:0004714"(transmembrane receptor protein tyrosine kinase activity)|go:"GO:0005078"(MAP-kinase scaffold activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005634"(nucleus)|go:"GO:0005739"(mitochondrion)|go:"GO:0005769"(early endosome)|go:"GO:0005770"(late endosome)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005815"(microtubule organizing center)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0005925"(focal adhesion)|go:"GO:0006468"(protein phosphorylation)|go:"GO:0006935"(chemotaxis)|go:"GO:0007165"(signal transduction)|go:"GO:0007507"(heart development)|go:"GO:0008022"(protein C-terminus binding)|go:"GO:0008285"(negative regulation of cell population proliferation)|go:"GO:0010628"(positive regulation of gene expression)|go:"GO:0018107"(peptidyl-threonine phosphorylation)|go:"GO:0021697"(cerebellar cortex formation)|go:"GO:0030182"(neuron differentiation)|go:"GO:0030216"(keratinocyte differentiation)|go:"GO:0030878"(thyroid gland development)|go:"GO:0032872"(regulation of stress-activated MAPK cascade)|go:"GO:0043539"(protein serine/threonine kinase activator activity)|go:"GO:0045893"(positive regulation of transcription, DNA-templated)|go:"GO:0047485"(protein N-terminus binding)|go:"GO:0048538"(thymus development)|go:"GO:0048679"(regulation of axon regeneration)|go:"GO:0048870"(cell motility)|go:"GO:0050772"(positive regulation of axonogenesis)|go:"GO:0051726"(regulation of cell cycle)|go:"GO:0060020"(Bergmann glial cell differentiation)|go:"GO:0060324"(face development)|go:"GO:0060440"(trachea formation)|go:"GO:0060502"(epithelial cell proliferation involved in lung morphogenesis)|go:"GO:0060674"(placenta blood vessel development)|go:"GO:0060711"(labyrinthine layer development)|go:"GO:0070371"(ERK1 and ERK2 cascade)|go:"GO:0070374"(positive regulation of ERK1 and ERK2 cascade)|go:"GO:0071902"(positive regulation of protein serine/threonine kinase activity)|go:"GO:0090170"(regulation of Golgi inheritance)|go:"GO:0090398"(cellular senescence)|go:"GO:0097110"(scaffold protein binding)|go:"GO:0106310"(protein serine kinase activity)|go:"GO:0106311"|go:"GO:1903800"(positive regulation of production of miRNAs involved in gene silencing by miRNA)|go:"GO:2000641"(regulation of early endosome to late endosome transport)|interpro:IPR000719(Protein kinase, core)|interpro:IPR008271(Serine/threonine protein kinase, active site)|interpro:IPR011009(Protein kinase-like)|interpro:IPR017441(Protein kinase ATP binding, conserved site)|mint:Q02750|rcsb pdb:1S9J|rcsb pdb:2P55|rcsb pdb:3DV3|rcsb pdb:3DY7|rcsb pdb:3E8N|rcsb pdb:3EQB|rcsb pdb:3EQC|rcsb pdb:3EQD|rcsb pdb:3EQF|rcsb pdb:3EQG|rcsb pdb:3EQH|rcsb pdb:3EQI|rcsb pdb:3MBL|rcsb pdb:3ORN|rcsb pdb:3OS3|rcsb pdb:3PP1|rcsb pdb:3SLS|rcsb pdb:3V01|rcsb pdb:3V04|rcsb pdb:3VVH|rcsb pdb:3W8Q|rcsb pdb:3WIG|rcsb pdb:3ZLS|rcsb pdb:3ZLW|rcsb pdb:3ZLX|rcsb pdb:3ZLY|rcsb pdb:3ZM4|rcsb pdb:4AN2|rcsb pdb:4AN3|rcsb pdb:4AN9|rcsb pdb:4ANB|rcsb pdb:4ARK|rcsb pdb:4LMN|rcsb pdb:4MNE|rcsb pdb:4U7Z|rcsb pdb:4U80|rcsb pdb:4U81|rcsb pdb:5EYM|rcsb pdb:5HZE|rcsb pdb:5YT3|rcsb pdb:6NYB|rcsb pdb:6PP9|rcsb pdb:6Q0J|rcsb pdb:6Q0T|rcsb pdb:6U2G|rcsb pdb:6V2W|rcsb pdb:6X2P|rcsb pdb:6X2S|rcsb pdb:6X2X|rcsb pdb:7B3M|rcsb pdb:7B7R|rcsb pdb:7B94|rcsb pdb:7B9L|rcsb pdb:7M0T|rcsb pdb:7M0U|rcsb pdb:7M0V|rcsb pdb:7M0W|rcsb pdb:7M0X|rcsb pdb:7M0Y|rcsb pdb:7M0Z|reactome:R-HSA-110056|reactome:R-HSA-170968|reactome:R-HSA-445144|reactome:R-HSA-5210891|reactome:R-HSA-5673000|reactome:R-HSA-5674135|reactome:R-HSA-5674499|reactome:R-HSA-5684264|reactome:R-HSA-6802946|reactome:R-HSA-6802948|reactome:R-HSA-6802952|reactome:R-HSA-6802955|reactome:R-HSA-9649948|reactome:R-HSA-9652169|reactome:R-HSA-9656223|rcsb pdb:5BX0	ensembl:ENSG00000157764(gene)|ensembl:ENST00000646891(transcript)|go:"GO:0000165"(MAPK cascade)|go:"GO:0004672"(protein kinase activity)|go:"GO:0004674"(protein serine/threonine kinase activity)|go:"GO:0004708"(MAP kinase kinase activity)|go:"GO:0005509"(calcium ion binding)|go:"GO:0005524"(ATP binding)|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0006468"(protein phosphorylation)|go:"GO:0007173"(epidermal growth factor receptor signaling pathway)|go:"GO:0009887"(animal organ morphogenesis)|go:"GO:0010628"(positive regulation of gene expression)|go:"GO:0010828"(positive regulation of glucose transmembrane transport)|go:"GO:0033138"(positive regulation of peptidyl-serine phosphorylation)|rcsb pdb:7M0T|rcsb pdb:7M0U|rcsb pdb:7M0V|go:"GO:0042802"(identical protein binding)|rcsb pdb:7M0W|rcsb pdb:7M0X|rcsb pdb:7M0Y|rcsb pdb:7M0Z|reactome:R-HSA-1295596|reactome:R-HSA-170968|reactome:R-HSA-170984|reactome:R-HSA-187706|reactome:R-HSA-5673000|reactome:R-HSA-5674135|reactome:R-HSA-5674499|reactome:R-HSA-5675221|reactome:R-HSA-6802946|reactome:R-HSA-6802948|reactome:R-HSA-6802952|reactome:R-HSA-6802955|reactome:R-HSA-9649948|reactome:R-HSA-9656223|reactome:R-HSA-9660537|rcsb pdb:4FK3|rcsb pdb:4G9C|rcsb pdb:4G9R|rcsb pdb:4H58|rcsb pdb:4JVG|rcsb pdb:4KSP|rcsb pdb:4KSQ|rcsb pdb:4FC0|rcsb pdb:4MBJ|rcsb pdb:4MNE|rcsb pdb:4MNF|rcsb pdb:4PP7|rcsb pdb:4R5Y|rcsb pdb:4RZV|rcsb pdb:4RZW|rcsb pdb:4WO5|rcsb pdb:4XV1|rcsb pdb:4XV2|rcsb pdb:4XV3|rcsb pdb:4XV9|rcsb pdb:4YHT|rcsb pdb:5C9C|rcsb pdb:5CSW|rcsb pdb:5CSX|rcsb pdb:5CT7|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0070374"(positive regulation of ERK1 and ERK2 cascade)|go:"GO:0043066"(negative regulation of apoptotic process)|go:"GO:0070413"(trehalose metabolism in response to stress)|go:"GO:0071277"(cellular response to calcium ion)|go:"GO:0090150"(establishment of protein localization to membrane)|go:"GO:0097110"(scaffold protein binding)|go:"GO:0106310"(protein serine kinase activity)|go:"GO:0106311"|interpro:IPR000719(Protein kinase, core)|interpro:IPR001245(Tyrosine protein kinase)|interpro:IPR002219(Protein kinase C, phorbol ester/diacylglycerol binding)|interpro:IPR003116(Raf-like Ras-binding)|interpro:IPR008271(Serine/threonine protein kinase, active site)|interpro:IPR011009(Protein kinase-like)|interpro:IPR017441(Protein kinase ATP binding, conserved site)|interpro:IPR020454|rcsb pdb:5FD2|rcsb pdb:5HI2|rcsb pdb:5HID|rcsb pdb:5HIE|rcsb pdb:5ITA|rcsb pdb:5J17|rcsb pdb:5J18|rcsb pdb:5J2R|rcsb pdb:5JRQ|rcsb pdb:5JSM|rcsb pdb:5JT2|rcsb pdb:5VAL|rcsb pdb:5VAM|rcsb pdb:5VR3|rcsb pdb:5VYK|rcsb pdb:6B8U|rcsb pdb:6CAD|rcsb pdb:6N0P|rcsb pdb:6N0Q|rcsb pdb:6NSQ|rcsb pdb:6NYB|rcsb pdb:6P3D|rcsb pdb:6P7G|rcsb pdb:6PP9|rcsb pdb:6Q0J|rcsb pdb:6Q0K|rcsb pdb:6Q0T|rcsb pdb:6U2G|rcsb pdb:6U2H|rcsb pdb:6UAN|rcsb pdb:6UUO|rcsb pdb:6V2U|rcsb pdb:6V2W|rcsb pdb:6XAG|mint:P15056|rcsb pdb:1UWH|rcsb pdb:1UWJ|rcsb pdb:2FB8|rcsb pdb:2L05|rcsb pdb:3C4C|interpro:IPR029071|rcsb pdb:3D4Q|rcsb pdb:3IDP|rcsb pdb:3II5|rcsb pdb:3NY5|rcsb pdb:3OG7|rcsb pdb:3PPJ|rcsb pdb:3PPK|rcsb pdb:3PRF|rcsb pdb:3PRI|rcsb pdb:3PSB|rcsb pdb:3PSD|rcsb pdb:3Q4C|rcsb pdb:3Q96|rcsb pdb:3SKC|rcsb pdb:3TV4|rcsb pdb:3TV6|rcsb pdb:4CQE|rcsb pdb:4DBN|rcsb pdb:4E26|rcsb pdb:4E4X|rcsb pdb:4EHE|rcsb pdb:4EHG|refseq:NP_004324.2|ensembl:ENSP00000493543|dip:DIP-1045N	-	-	-	figure legend:4 A/D, 6A, 7G, Supp  figure 4 A|agonist:"MEK inhibitors: Selumetinib and PD0325901  CH5126766 - a newer MEK inhibitor."|antagonist:Trametinib -a newer MEK inhibitor, disrupts the interaction of MEK1 with ARAF and CRAF.|comment:In cells co-transfected with K-RAS G12V   this interaction was completely disrupted, but was only partially reduced in the presence of CH5126766 or PD0325901.  Co-transfection of BRAF V600E reduced this interaction,and  the presence of CH5126766 or PD0325901 further reduced the interaction , but in the presence of CH5126766 the interaction was increased.|dataset:Cancer - Interactions investigated in the context of cancer|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:9606(human-293)|taxid:9606(Homo sapiens transformed primary embryonal kidney cells)	-	2014/06/12	2015/05/15	rogid:ieBLcDINn9hZFZZEIQ3Ktdf2NF89606	rogid:sfzILpNpX8UFB/vgH9LOKLpl/+g9606	intact-crc:695C8F4CF92B4292|rigid:VLl+5T+aNh8kZKnWh1WwvnrN0PI	false	flag tag:?-?|mutation decreasing interaction:218-218,222-222|mutation disrupting interaction:308-308,310-310	-	-	-	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)
uniprotkb:P36507	uniprotkb:P10398	intact:EBI-1056930|ensembl:ENSP00000262948|intact:EBI-15586042	intact:EBI-365961|uniprotkb:P07557|uniprotkb:Q5H9B3|intact:EBI-28988863|ensembl:ENSP00000366244|uniprotkb:Q5H9B2	psi-mi:mp2k2_human(display_long)|uniprotkb:MAP2K2(gene name)|psi-mi:MAP2K2(display_short)|uniprotkb:MEK2(gene name synonym)|uniprotkb:MKK2(gene name synonym)|uniprotkb:PRKMK2(gene name synonym)|uniprotkb:ERK activator kinase 2(gene name synonym)|uniprotkb:MAPK/ERK kinase 2(gene name synonym)	psi-mi:araf_human(display_long)|uniprotkb:ARAF(gene name)|psi-mi:ARAF(display_short)|uniprotkb:PKS(gene name synonym)|uniprotkb:PKS2(gene name synonym)|uniprotkb:ARAF1(gene name synonym)|uniprotkb:Proto-oncogene A-Raf-1(gene name synonym)|uniprotkb:Proto-oncogene Pks(gene name synonym)|uniprotkb:Proto-oncogene A-Raf(gene name synonym)	psi-mi:"MI:0007"(anti tag coimmunoprecipitation)	Lito et al. (2014)	pubmed:24746704|imex:IM-22983	taxid:9606(human)|taxid:9606(Homo sapiens)	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-9550104|imex:IM-22983-7	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_109587.1|ensembl:ENSG00000126934(gene)|ensembl:ENST00000262948(transcript)|go:"GO:0000187"(obsolete activation of MAPK activity)|go:"GO:0004674"(protein serine/threonine kinase activity)|go:"GO:0004708"(MAP kinase kinase activity)|go:"GO:0004712"(protein serine/threonine/tyrosine kinase activity)|go:"GO:0004714"(transmembrane receptor protein tyrosine kinase activity)|go:"GO:0005078"(MAP-kinase scaffold activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005576"(extracellular region)|go:"GO:0005739"(mitochondrion)|go:"GO:0005769"(early endosome)|go:"GO:0005770"(late endosome)|go:"GO:0005778"(peroxisomal membrane)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005829"(cytosol)|go:"GO:0005874"(microtubule)|go:"GO:0005911"(cell-cell junction)|go:"GO:0005925"(focal adhesion)|go:"GO:0009898"(cytoplasmic side of plasma membrane)|go:"GO:0030165"(PDZ domain binding)|go:"GO:0032872"(regulation of stress-activated MAPK cascade)|go:"GO:0036289"(peptidyl-serine autophosphorylation)|go:"GO:0043539"(protein serine/threonine kinase activator activity)|go:"GO:0045893"(positive regulation of transcription, DNA-templated)|go:"GO:0046872"(metal ion binding)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0070371"(ERK1 and ERK2 cascade)|go:"GO:0071902"(positive regulation of protein serine/threonine kinase activity)|go:"GO:0090170"(regulation of Golgi inheritance)|go:"GO:0097110"(scaffold protein binding)|go:"GO:0106310"(protein serine kinase activity)|go:"GO:0106311"|go:"GO:1903800"(positive regulation of production of miRNAs involved in gene silencing by miRNA)|go:"GO:2000641"(regulation of early endosome to late endosome transport)|interpro:IPR000719(Protein kinase, core)|interpro:IPR008271(Serine/threonine protein kinase, active site)|interpro:IPR011009(Protein kinase-like)|interpro:IPR017441(Protein kinase ATP binding, conserved site)|mint:P36507|rcsb pdb:1S9I|rcsb pdb:4H3Q|reactome:R-HSA-112411|reactome:R-HSA-170968|reactome:R-HSA-445144|reactome:R-HSA-5210891|reactome:R-HSA-5673000|reactome:R-HSA-5674135|reactome:R-HSA-5674499|reactome:R-HSA-6802946|reactome:R-HSA-6802948|reactome:R-HSA-6802952|reactome:R-HSA-6802955|reactome:R-HSA-9649948|reactome:R-HSA-9652169|reactome:R-HSA-9656223|go:"GO:0005634"(nucleus)|dip:DIP-29119N	ensembl:ENSG00000078061(gene)|ensembl:ENST00000377045(transcript)|go:"GO:0004672"(protein kinase activity)|go:"GO:0004674"(protein serine/threonine kinase activity)|go:"GO:0004709"(MAP kinase kinase kinase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005739"(mitochondrion)|go:"GO:0005829"(cytosol)|go:"GO:0006464"(cellular protein modification process)|go:"GO:0032006"(regulation of TOR signaling)|go:"GO:0032434"(regulation of proteasomal ubiquitin-dependent protein catabolic process)|go:"GO:0033138"(positive regulation of peptidyl-serine phosphorylation)|go:"GO:0043066"(negative regulation of apoptotic process)|go:"GO:0046872"(metal ion binding)|go:"GO:0106310"(protein serine kinase activity)|go:"GO:0106311"|interpro:IPR000719(Protein kinase, core)|interpro:IPR001245(Tyrosine protein kinase)|interpro:IPR002219(Protein kinase C, phorbol ester/diacylglycerol binding)|interpro:IPR003116(Raf-like Ras-binding)|interpro:IPR008271(Serine/threonine protein kinase, active site)|interpro:IPR011009(Protein kinase-like)|interpro:IPR017441(Protein kinase ATP binding, conserved site)|interpro:IPR020454|interpro:IPR029071|mint:P10398|rcsb pdb:2MSE|reactome:R-HSA-5673000|reactome:R-HSA-5674135|reactome:R-HSA-5675221|reactome:R-HSA-6802946|reactome:R-HSA-6802948|reactome:R-HSA-6802952|reactome:R-HSA-6802955|reactome:R-HSA-9649948|reactome:R-HSA-9656223|reactome:R-HSA-9660537|rcsb pdb:1WXM|refseq:NP_001645.1|refseq:XP_011542210.1|refseq:NP_001243125.1|ensembl:ENST00000377039|ensembl:ENSP00000366238|ensembl:ENSP00000366244|refseq:NP_001243126.1|dip:DIP-31374N	-	-	-	agonist:"CH5126766 - a newer MEK inhibitor. (PD0325901)"|comment:"K-RAS G12V transfection:- this interaction was completely disrupted, but was only partially reduced in the presence of CH5126766 or PD0325901."|comment:"BRAF V600E transfection: MEK2 interacted with ARAF, BRAF and CRAF,  but no untransfected control result is shown.  The presence of  PD0325901 did not appear to affect   this interaction.   In the presence of  Trametinib  the interaction of MEK2 and BRAF still took place at a reduced level.  It is not clear if any  of these interactions   were  with endogenous wild-type BRAF, or the V600E mutant, or both."|figure legend:Supp fig 4B|antagonist:Trametinib -a newer MEK inhibitor, disrupts the interaction of MEK2 with ARAF and CRAF.|dataset:Cancer - Interactions investigated in the context of cancer|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:9606(human-293)|taxid:9606(Homo sapiens transformed primary embryonal kidney cells)	-	2014/06/12	2014/10/16	rogid:JnGMm7dA0eZgBV4A/n+ZjE8ATq49606	rogid:WRtbdrEuDxNkA6jRRxvapodFcUE9606	intact-crc:776512D0EC49DD06|rigid:ten3XimtWbBZwq7FBJktem99ve0	false	flag tag:?-?	-	-	-	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)
uniprotkb:P36507	uniprotkb:P15056	intact:EBI-1056930|ensembl:ENSP00000262948|intact:EBI-15586042	intact:EBI-365980|uniprotkb:Q13878|uniprotkb:Q3MIN6|intact:EBI-28988924|ensembl:ENSP00000493543|uniprotkb:Q9UDP8|uniprotkb:Q9Y6T3|uniprotkb:A4D1T4|uniprotkb:B6HY61|uniprotkb:B6HY62|uniprotkb:B6HY63|uniprotkb:B6HY64|uniprotkb:B6HY65|uniprotkb:B6HY66	psi-mi:mp2k2_human(display_long)|uniprotkb:MAP2K2(gene name)|psi-mi:MAP2K2(display_short)|uniprotkb:MEK2(gene name synonym)|uniprotkb:MKK2(gene name synonym)|uniprotkb:PRKMK2(gene name synonym)|uniprotkb:ERK activator kinase 2(gene name synonym)|uniprotkb:MAPK/ERK kinase 2(gene name synonym)	psi-mi:braf_human(display_long)|uniprotkb:BRAF(gene name)|psi-mi:BRAF(display_short)|uniprotkb:BRAF1(gene name synonym)|uniprotkb:RAFB1(gene name synonym)|uniprotkb:v-Raf murine sarcoma viral oncogene homolog B1(gene name synonym)|uniprotkb:p94(gene name synonym)|uniprotkb:Proto-oncogene B-Raf(gene name synonym)	psi-mi:"MI:0007"(anti tag coimmunoprecipitation)	Lito et al. (2014)	pubmed:24746704|imex:IM-22983	taxid:9606(human)|taxid:9606(Homo sapiens)	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-9550104|imex:IM-22983-7	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_109587.1|ensembl:ENSG00000126934(gene)|ensembl:ENST00000262948(transcript)|go:"GO:0000187"(obsolete activation of MAPK activity)|go:"GO:0004674"(protein serine/threonine kinase activity)|go:"GO:0004708"(MAP kinase kinase activity)|go:"GO:0004712"(protein serine/threonine/tyrosine kinase activity)|go:"GO:0004714"(transmembrane receptor protein tyrosine kinase activity)|go:"GO:0005078"(MAP-kinase scaffold activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005576"(extracellular region)|go:"GO:0005739"(mitochondrion)|go:"GO:0005769"(early endosome)|go:"GO:0005770"(late endosome)|go:"GO:0005778"(peroxisomal membrane)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005829"(cytosol)|go:"GO:0005874"(microtubule)|go:"GO:0005911"(cell-cell junction)|go:"GO:0005925"(focal adhesion)|go:"GO:0009898"(cytoplasmic side of plasma membrane)|go:"GO:0030165"(PDZ domain binding)|go:"GO:0032872"(regulation of stress-activated MAPK cascade)|go:"GO:0036289"(peptidyl-serine autophosphorylation)|go:"GO:0043539"(protein serine/threonine kinase activator activity)|go:"GO:0045893"(positive regulation of transcription, DNA-templated)|go:"GO:0046872"(metal ion binding)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0070371"(ERK1 and ERK2 cascade)|go:"GO:0071902"(positive regulation of protein serine/threonine kinase activity)|go:"GO:0090170"(regulation of Golgi inheritance)|go:"GO:0097110"(scaffold protein binding)|go:"GO:0106310"(protein serine kinase activity)|go:"GO:0106311"|go:"GO:1903800"(positive regulation of production of miRNAs involved in gene silencing by miRNA)|go:"GO:2000641"(regulation of early endosome to late endosome transport)|interpro:IPR000719(Protein kinase, core)|interpro:IPR008271(Serine/threonine protein kinase, active site)|interpro:IPR011009(Protein kinase-like)|interpro:IPR017441(Protein kinase ATP binding, conserved site)|mint:P36507|rcsb pdb:1S9I|rcsb pdb:4H3Q|reactome:R-HSA-112411|reactome:R-HSA-170968|reactome:R-HSA-445144|reactome:R-HSA-5210891|reactome:R-HSA-5673000|reactome:R-HSA-5674135|reactome:R-HSA-5674499|reactome:R-HSA-6802946|reactome:R-HSA-6802948|reactome:R-HSA-6802952|reactome:R-HSA-6802955|reactome:R-HSA-9649948|reactome:R-HSA-9652169|reactome:R-HSA-9656223|go:"GO:0005634"(nucleus)|dip:DIP-29119N	ensembl:ENSG00000157764(gene)|ensembl:ENST00000646891(transcript)|go:"GO:0000165"(MAPK cascade)|go:"GO:0004672"(protein kinase activity)|go:"GO:0004674"(protein serine/threonine kinase activity)|go:"GO:0004708"(MAP kinase kinase activity)|go:"GO:0005509"(calcium ion binding)|go:"GO:0005524"(ATP binding)|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0006468"(protein phosphorylation)|go:"GO:0007173"(epidermal growth factor receptor signaling pathway)|go:"GO:0009887"(animal organ morphogenesis)|go:"GO:0010628"(positive regulation of gene expression)|go:"GO:0010828"(positive regulation of glucose transmembrane transport)|go:"GO:0033138"(positive regulation of peptidyl-serine phosphorylation)|rcsb pdb:7M0T|rcsb pdb:7M0U|rcsb pdb:7M0V|go:"GO:0042802"(identical protein binding)|rcsb pdb:7M0W|rcsb pdb:7M0X|rcsb pdb:7M0Y|rcsb pdb:7M0Z|reactome:R-HSA-1295596|reactome:R-HSA-170968|reactome:R-HSA-170984|reactome:R-HSA-187706|reactome:R-HSA-5673000|reactome:R-HSA-5674135|reactome:R-HSA-5674499|reactome:R-HSA-5675221|reactome:R-HSA-6802946|reactome:R-HSA-6802948|reactome:R-HSA-6802952|reactome:R-HSA-6802955|reactome:R-HSA-9649948|reactome:R-HSA-9656223|reactome:R-HSA-9660537|rcsb pdb:4FK3|rcsb pdb:4G9C|rcsb pdb:4G9R|rcsb pdb:4H58|rcsb pdb:4JVG|rcsb pdb:4KSP|rcsb pdb:4KSQ|rcsb pdb:4FC0|rcsb pdb:4MBJ|rcsb pdb:4MNE|rcsb pdb:4MNF|rcsb pdb:4PP7|rcsb pdb:4R5Y|rcsb pdb:4RZV|rcsb pdb:4RZW|rcsb pdb:4WO5|rcsb pdb:4XV1|rcsb pdb:4XV2|rcsb pdb:4XV3|rcsb pdb:4XV9|rcsb pdb:4YHT|rcsb pdb:5C9C|rcsb pdb:5CSW|rcsb pdb:5CSX|rcsb pdb:5CT7|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0070374"(positive regulation of ERK1 and ERK2 cascade)|go:"GO:0043066"(negative regulation of apoptotic process)|go:"GO:0070413"(trehalose metabolism in response to stress)|go:"GO:0071277"(cellular response to calcium ion)|go:"GO:0090150"(establishment of protein localization to membrane)|go:"GO:0097110"(scaffold protein binding)|go:"GO:0106310"(protein serine kinase activity)|go:"GO:0106311"|interpro:IPR000719(Protein kinase, core)|interpro:IPR001245(Tyrosine protein kinase)|interpro:IPR002219(Protein kinase C, phorbol ester/diacylglycerol binding)|interpro:IPR003116(Raf-like Ras-binding)|interpro:IPR008271(Serine/threonine protein kinase, active site)|interpro:IPR011009(Protein kinase-like)|interpro:IPR017441(Protein kinase ATP binding, conserved site)|interpro:IPR020454|rcsb pdb:5FD2|rcsb pdb:5HI2|rcsb pdb:5HID|rcsb pdb:5HIE|rcsb pdb:5ITA|rcsb pdb:5J17|rcsb pdb:5J18|rcsb pdb:5J2R|rcsb pdb:5JRQ|rcsb pdb:5JSM|rcsb pdb:5JT2|rcsb pdb:5VAL|rcsb pdb:5VAM|rcsb pdb:5VR3|rcsb pdb:5VYK|rcsb pdb:6B8U|rcsb pdb:6CAD|rcsb pdb:6N0P|rcsb pdb:6N0Q|rcsb pdb:6NSQ|rcsb pdb:6NYB|rcsb pdb:6P3D|rcsb pdb:6P7G|rcsb pdb:6PP9|rcsb pdb:6Q0J|rcsb pdb:6Q0K|rcsb pdb:6Q0T|rcsb pdb:6U2G|rcsb pdb:6U2H|rcsb pdb:6UAN|rcsb pdb:6UUO|rcsb pdb:6V2U|rcsb pdb:6V2W|rcsb pdb:6XAG|mint:P15056|rcsb pdb:1UWH|rcsb pdb:1UWJ|rcsb pdb:2FB8|rcsb pdb:2L05|rcsb pdb:3C4C|interpro:IPR029071|rcsb pdb:3D4Q|rcsb pdb:3IDP|rcsb pdb:3II5|rcsb pdb:3NY5|rcsb pdb:3OG7|rcsb pdb:3PPJ|rcsb pdb:3PPK|rcsb pdb:3PRF|rcsb pdb:3PRI|rcsb pdb:3PSB|rcsb pdb:3PSD|rcsb pdb:3Q4C|rcsb pdb:3Q96|rcsb pdb:3SKC|rcsb pdb:3TV4|rcsb pdb:3TV6|rcsb pdb:4CQE|rcsb pdb:4DBN|rcsb pdb:4E26|rcsb pdb:4E4X|rcsb pdb:4EHE|rcsb pdb:4EHG|refseq:NP_004324.2|ensembl:ENSP00000493543|dip:DIP-1045N	-	-	-	agonist:"CH5126766 - a newer MEK inhibitor. (PD0325901)"|comment:"K-RAS G12V transfection:- this interaction was completely disrupted, but was only partially reduced in the presence of CH5126766 or PD0325901."|comment:"BRAF V600E transfection: MEK2 interacted with ARAF, BRAF and CRAF,  but no untransfected control result is shown.  The presence of  PD0325901 did not appear to affect   this interaction.   In the presence of  Trametinib  the interaction of MEK2 and BRAF still took place at a reduced level.  It is not clear if any  of these interactions   were  with endogenous wild-type BRAF, or the V600E mutant, or both."|figure legend:Supp fig 4B|antagonist:Trametinib -a newer MEK inhibitor, disrupts the interaction of MEK2 with ARAF and CRAF.|dataset:Cancer - Interactions investigated in the context of cancer|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:9606(human-293)|taxid:9606(Homo sapiens transformed primary embryonal kidney cells)	-	2014/06/12	2014/10/16	rogid:JnGMm7dA0eZgBV4A/n+ZjE8ATq49606	rogid:sfzILpNpX8UFB/vgH9LOKLpl/+g9606	intact-crc:776512D0EC49DD06|rigid:ten3XimtWbBZwq7FBJktem99ve0	false	flag tag:?-?	-	-	-	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)
uniprotkb:P36507	uniprotkb:P04049	intact:EBI-1056930|ensembl:ENSP00000262948|intact:EBI-15586042	intact:EBI-365996|uniprotkb:B0LPH8|uniprotkb:Q15278|uniprotkb:Q9UC20|ensembl:ENSP00000251849|uniprotkb:B2R5N3	psi-mi:mp2k2_human(display_long)|uniprotkb:MAP2K2(gene name)|psi-mi:MAP2K2(display_short)|uniprotkb:MEK2(gene name synonym)|uniprotkb:MKK2(gene name synonym)|uniprotkb:PRKMK2(gene name synonym)|uniprotkb:ERK activator kinase 2(gene name synonym)|uniprotkb:MAPK/ERK kinase 2(gene name synonym)	psi-mi:raf1_human(display_long)|uniprotkb:RAF1(gene name)|psi-mi:RAF1(display_short)|uniprotkb:RAF(gene name synonym)|uniprotkb:Proto-oncogene c-RAF(gene name synonym)|uniprotkb:Raf-1(gene name synonym)	psi-mi:"MI:0007"(anti tag coimmunoprecipitation)	Lito et al. (2014)	pubmed:24746704|imex:IM-22983	taxid:9606(human)|taxid:9606(Homo sapiens)	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-9550104|imex:IM-22983-7	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_109587.1|ensembl:ENSG00000126934(gene)|ensembl:ENST00000262948(transcript)|go:"GO:0000187"(obsolete activation of MAPK activity)|go:"GO:0004674"(protein serine/threonine kinase activity)|go:"GO:0004708"(MAP kinase kinase activity)|go:"GO:0004712"(protein serine/threonine/tyrosine kinase activity)|go:"GO:0004714"(transmembrane receptor protein tyrosine kinase activity)|go:"GO:0005078"(MAP-kinase scaffold activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005576"(extracellular region)|go:"GO:0005739"(mitochondrion)|go:"GO:0005769"(early endosome)|go:"GO:0005770"(late endosome)|go:"GO:0005778"(peroxisomal membrane)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005829"(cytosol)|go:"GO:0005874"(microtubule)|go:"GO:0005911"(cell-cell junction)|go:"GO:0005925"(focal adhesion)|go:"GO:0009898"(cytoplasmic side of plasma membrane)|go:"GO:0030165"(PDZ domain binding)|go:"GO:0032872"(regulation of stress-activated MAPK cascade)|go:"GO:0036289"(peptidyl-serine autophosphorylation)|go:"GO:0043539"(protein serine/threonine kinase activator activity)|go:"GO:0045893"(positive regulation of transcription, DNA-templated)|go:"GO:0046872"(metal ion binding)|go:"GO:0048471"(perinuclear region of cytoplasm)|go:"GO:0070371"(ERK1 and ERK2 cascade)|go:"GO:0071902"(positive regulation of protein serine/threonine kinase activity)|go:"GO:0090170"(regulation of Golgi inheritance)|go:"GO:0097110"(scaffold protein binding)|go:"GO:0106310"(protein serine kinase activity)|go:"GO:0106311"|go:"GO:1903800"(positive regulation of production of miRNAs involved in gene silencing by miRNA)|go:"GO:2000641"(regulation of early endosome to late endosome transport)|interpro:IPR000719(Protein kinase, core)|interpro:IPR008271(Serine/threonine protein kinase, active site)|interpro:IPR011009(Protein kinase-like)|interpro:IPR017441(Protein kinase ATP binding, conserved site)|mint:P36507|rcsb pdb:1S9I|rcsb pdb:4H3Q|reactome:R-HSA-112411|reactome:R-HSA-170968|reactome:R-HSA-445144|reactome:R-HSA-5210891|reactome:R-HSA-5673000|reactome:R-HSA-5674135|reactome:R-HSA-5674499|reactome:R-HSA-6802946|reactome:R-HSA-6802948|reactome:R-HSA-6802952|reactome:R-HSA-6802955|reactome:R-HSA-9649948|reactome:R-HSA-9652169|reactome:R-HSA-9656223|go:"GO:0005634"(nucleus)|dip:DIP-29119N	rcsb pdb:3IQV|rcsb pdb:3KUC|rcsb pdb:3KUD|rcsb pdb:3NKX|rcsb pdb:3O8I|rcsb pdb:3OMV|rcsb pdb:4FJ3|rcsb pdb:4G0N|rcsb pdb:4G3X|rcsb pdb:4IEA|rcsb pdb:4IHL|rcsb pdb:6NTC|rcsb pdb:6NTD|rcsb pdb:6PTS|rcsb pdb:6PTW|rcsb pdb:6VJJ|rcsb pdb:6XGU|rcsb pdb:6XGV|rcsb pdb:6XHA|rcsb pdb:6XHB|rcsb pdb:6XI7|ensembl:ENSG00000132155(gene)|ensembl:ENST00000251849(transcript)|go:"GO:0000186"(obsolete activation of MAPKK activity)|go:"GO:0001666"(response to hypoxia)|go:"GO:0004672"(protein kinase activity)|go:"GO:0004674"(protein serine/threonine kinase activity)|go:"GO:0004709"(MAP kinase kinase kinase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005737"(cytoplasm)|go:"GO:0005739"(mitochondrion)|go:"GO:0005741"(mitochondrial outer membrane)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0006468"(protein phosphorylation)|go:"GO:0006915"(apoptotic process)|go:"GO:0007165"(signal transduction)|go:"GO:0007190"(activation of adenylate cyclase activity)|go:"GO:0007507"(heart development)|go:"GO:0008179"(adenylate cyclase binding)|go:"GO:0008285"(negative regulation of cell population proliferation)|go:"GO:0010856"(adenylate cyclase activator activity)|go:"GO:0016607"(nuclear speck)|go:"GO:0019899"(enzyme binding)|go:"GO:0030154"(cell differentiation)|go:"GO:0030878"(thyroid gland development)|go:"GO:0031143"(pseudopodium)|go:"GO:0031333"(negative regulation of protein-containing complex assembly)|go:"GO:0031434"(mitogen-activated protein kinase kinase binding)|go:"GO:0033138"(positive regulation of peptidyl-serine phosphorylation)|go:"GO:0035019"(somatic stem cell population maintenance)|go:"GO:0035023"(regulation of Rho protein signal transduction)|go:"GO:0035773"(insulin secretion involved in cellular response to glucose stimulus)|go:"GO:0035994"(response to muscle stretch)|go:"GO:0042060"(wound healing)|go:"GO:0042802"(identical protein binding)|go:"GO:0042981"(regulation of apoptotic process)|go:"GO:0043066"(negative regulation of apoptotic process)|go:"GO:0043154"(negative regulation of cysteine-type endopeptidase activity involved in apoptotic process)|go:"GO:0044877"(protein-containing complex binding)|go:"GO:0045104"(intermediate filament cytoskeleton organization)|go:"GO:0045595"(regulation of cell differentiation)|go:"GO:0045944"(positive regulation of transcription by RNA polymerase II)|go:"GO:0046872"(metal ion binding)|go:"GO:0048011"(neurotrophin TRK receptor signaling pathway)|go:"GO:0048538"(thymus development)|go:"GO:0060324"(face development)|go:"GO:0071550"(death-inducing signaling complex assembly)|go:"GO:0106310"(protein serine kinase activity)|go:"GO:0106311"|go:"GO:1902042"(negative regulation of extrinsic apoptotic signaling pathway via death domain receptors)|go:"GO:2000145"(regulation of cell motility)|interpro:IPR000719(Protein kinase, core)|interpro:IPR001245(Tyrosine protein kinase)|interpro:IPR002219(Protein kinase C, phorbol ester/diacylglycerol binding)|interpro:IPR003116(Raf-like Ras-binding)|interpro:IPR008271(Serine/threonine protein kinase, active site)|interpro:IPR011009(Protein kinase-like)|interpro:IPR017441(Protein kinase ATP binding, conserved site)|interpro:IPR020454|interpro:IPR029071|mint:P04049|rcsb pdb:1C1Y|rcsb pdb:1FAQ|rcsb pdb:1FAR|rcsb pdb:1GUA|rcsb pdb:1RFA|rcsb pdb:3CU8|rcsb pdb:3IQJ|rcsb pdb:3IQU|rcsb pdb:7JHP|reactome:R-HSA-2672351|reactome:R-HSA-392517|reactome:R-HSA-430116|reactome:R-HSA-5621575|reactome:R-HSA-5673000|reactome:R-HSA-5674135|reactome:R-HSA-5674499|reactome:R-HSA-5675221|reactome:R-HSA-6802946|reactome:R-HSA-6802948|reactome:R-HSA-6802952|reactome:R-HSA-6802955|reactome:R-HSA-9649948|reactome:R-HSA-9656223|reactome:R-HSA-9660537|refseq:NP_002871.1|refseq:XP_011532276.1|refseq:XP_005265412.1|dip:DIP-1048N	-	-	-	agonist:"CH5126766 - a newer MEK inhibitor. (PD0325901)"|comment:"K-RAS G12V transfection:- this interaction was completely disrupted, but was only partially reduced in the presence of CH5126766 or PD0325901."|comment:"BRAF V600E transfection: MEK2 interacted with ARAF, BRAF and CRAF,  but no untransfected control result is shown.  The presence of  PD0325901 did not appear to affect   this interaction.   In the presence of  Trametinib  the interaction of MEK2 and BRAF still took place at a reduced level.  It is not clear if any  of these interactions   were  with endogenous wild-type BRAF, or the V600E mutant, or both."|figure legend:Supp fig 4B|antagonist:Trametinib -a newer MEK inhibitor, disrupts the interaction of MEK2 with ARAF and CRAF.|dataset:Cancer - Interactions investigated in the context of cancer|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:9606(human-293)|taxid:9606(Homo sapiens transformed primary embryonal kidney cells)	-	2014/06/12	2014/10/16	rogid:JnGMm7dA0eZgBV4A/n+ZjE8ATq49606	rogid:1WOAPuzcZdMoq9hyFD/qr4Tz7049606	intact-crc:776512D0EC49DD06|rigid:ten3XimtWbBZwq7FBJktem99ve0	false	flag tag:?-?	-	-	-	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)
uniprotkb:Q02750	uniprotkb:P15056	intact:EBI-492564|ensembl:ENSP00000302486	intact:EBI-365980|uniprotkb:Q13878|uniprotkb:Q3MIN6|intact:EBI-28988924|ensembl:ENSP00000493543|uniprotkb:Q9UDP8|uniprotkb:Q9Y6T3|uniprotkb:A4D1T4|uniprotkb:B6HY61|uniprotkb:B6HY62|uniprotkb:B6HY63|uniprotkb:B6HY64|uniprotkb:B6HY65|uniprotkb:B6HY66	psi-mi:mp2k1_human(display_long)|uniprotkb:ERK activator kinase 1(gene name synonym)|uniprotkb:MAPK/ERK kinase 1(gene name synonym)|uniprotkb:MAP2K1(gene name)|psi-mi:MAP2K1(display_short)|uniprotkb:MEK1(gene name synonym)|uniprotkb:PRKMK1(gene name synonym)	psi-mi:braf_human(display_long)|uniprotkb:BRAF(gene name)|psi-mi:BRAF(display_short)|uniprotkb:BRAF1(gene name synonym)|uniprotkb:RAFB1(gene name synonym)|uniprotkb:v-Raf murine sarcoma viral oncogene homolog B1(gene name synonym)|uniprotkb:p94(gene name synonym)|uniprotkb:Proto-oncogene B-Raf(gene name synonym)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Lito et al. (2014)	pubmed:24746704|imex:IM-22983	taxid:9606(human)|taxid:9606(Homo sapiens)	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-9549280|imex:IM-22983-2	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_002746.1|refseq:XP_016877900.1|dip:DIP-201N|ensembl:ENSG00000169032(gene)|ensembl:ENST00000307102(transcript)|go:"GO:0000187"(obsolete activation of MAPK activity)|go:"GO:0004672"(protein kinase activity)|go:"GO:0004674"(protein serine/threonine kinase activity)|go:"GO:0004708"(MAP kinase kinase activity)|go:"GO:0004712"(protein serine/threonine/tyrosine kinase activity)|go:"GO:0004714"(transmembrane receptor protein tyrosine kinase activity)|go:"GO:0005078"(MAP-kinase scaffold activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005634"(nucleus)|go:"GO:0005739"(mitochondrion)|go:"GO:0005769"(early endosome)|go:"GO:0005770"(late endosome)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005815"(microtubule organizing center)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0005925"(focal adhesion)|go:"GO:0006468"(protein phosphorylation)|go:"GO:0006935"(chemotaxis)|go:"GO:0007165"(signal transduction)|go:"GO:0007507"(heart development)|go:"GO:0008022"(protein C-terminus binding)|go:"GO:0008285"(negative regulation of cell population proliferation)|go:"GO:0010628"(positive regulation of gene expression)|go:"GO:0018107"(peptidyl-threonine phosphorylation)|go:"GO:0021697"(cerebellar cortex formation)|go:"GO:0030182"(neuron differentiation)|go:"GO:0030216"(keratinocyte differentiation)|go:"GO:0030878"(thyroid gland development)|go:"GO:0032872"(regulation of stress-activated MAPK cascade)|go:"GO:0043539"(protein serine/threonine kinase activator activity)|go:"GO:0045893"(positive regulation of transcription, DNA-templated)|go:"GO:0047485"(protein N-terminus binding)|go:"GO:0048538"(thymus development)|go:"GO:0048679"(regulation of axon regeneration)|go:"GO:0048870"(cell motility)|go:"GO:0050772"(positive regulation of axonogenesis)|go:"GO:0051726"(regulation of cell cycle)|go:"GO:0060020"(Bergmann glial cell differentiation)|go:"GO:0060324"(face development)|go:"GO:0060440"(trachea formation)|go:"GO:0060502"(epithelial cell proliferation involved in lung morphogenesis)|go:"GO:0060674"(placenta blood vessel development)|go:"GO:0060711"(labyrinthine layer development)|go:"GO:0070371"(ERK1 and ERK2 cascade)|go:"GO:0070374"(positive regulation of ERK1 and ERK2 cascade)|go:"GO:0071902"(positive regulation of protein serine/threonine kinase activity)|go:"GO:0090170"(regulation of Golgi inheritance)|go:"GO:0090398"(cellular senescence)|go:"GO:0097110"(scaffold protein binding)|go:"GO:0106310"(protein serine kinase activity)|go:"GO:0106311"|go:"GO:1903800"(positive regulation of production of miRNAs involved in gene silencing by miRNA)|go:"GO:2000641"(regulation of early endosome to late endosome transport)|interpro:IPR000719(Protein kinase, core)|interpro:IPR008271(Serine/threonine protein kinase, active site)|interpro:IPR011009(Protein kinase-like)|interpro:IPR017441(Protein kinase ATP binding, conserved site)|mint:Q02750|rcsb pdb:1S9J|rcsb pdb:2P55|rcsb pdb:3DV3|rcsb pdb:3DY7|rcsb pdb:3E8N|rcsb pdb:3EQB|rcsb pdb:3EQC|rcsb pdb:3EQD|rcsb pdb:3EQF|rcsb pdb:3EQG|rcsb pdb:3EQH|rcsb pdb:3EQI|rcsb pdb:3MBL|rcsb pdb:3ORN|rcsb pdb:3OS3|rcsb pdb:3PP1|rcsb pdb:3SLS|rcsb pdb:3V01|rcsb pdb:3V04|rcsb pdb:3VVH|rcsb pdb:3W8Q|rcsb pdb:3WIG|rcsb pdb:3ZLS|rcsb pdb:3ZLW|rcsb pdb:3ZLX|rcsb pdb:3ZLY|rcsb pdb:3ZM4|rcsb pdb:4AN2|rcsb pdb:4AN3|rcsb pdb:4AN9|rcsb pdb:4ANB|rcsb pdb:4ARK|rcsb pdb:4LMN|rcsb pdb:4MNE|rcsb pdb:4U7Z|rcsb pdb:4U80|rcsb pdb:4U81|rcsb pdb:5EYM|rcsb pdb:5HZE|rcsb pdb:5YT3|rcsb pdb:6NYB|rcsb pdb:6PP9|rcsb pdb:6Q0J|rcsb pdb:6Q0T|rcsb pdb:6U2G|rcsb pdb:6V2W|rcsb pdb:6X2P|rcsb pdb:6X2S|rcsb pdb:6X2X|rcsb pdb:7B3M|rcsb pdb:7B7R|rcsb pdb:7B94|rcsb pdb:7B9L|rcsb pdb:7M0T|rcsb pdb:7M0U|rcsb pdb:7M0V|rcsb pdb:7M0W|rcsb pdb:7M0X|rcsb pdb:7M0Y|rcsb pdb:7M0Z|reactome:R-HSA-110056|reactome:R-HSA-170968|reactome:R-HSA-445144|reactome:R-HSA-5210891|reactome:R-HSA-5673000|reactome:R-HSA-5674135|reactome:R-HSA-5674499|reactome:R-HSA-5684264|reactome:R-HSA-6802946|reactome:R-HSA-6802948|reactome:R-HSA-6802952|reactome:R-HSA-6802955|reactome:R-HSA-9649948|reactome:R-HSA-9652169|reactome:R-HSA-9656223|rcsb pdb:5BX0	ensembl:ENSG00000157764(gene)|ensembl:ENST00000646891(transcript)|go:"GO:0000165"(MAPK cascade)|go:"GO:0004672"(protein kinase activity)|go:"GO:0004674"(protein serine/threonine kinase activity)|go:"GO:0004708"(MAP kinase kinase activity)|go:"GO:0005509"(calcium ion binding)|go:"GO:0005524"(ATP binding)|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0006468"(protein phosphorylation)|go:"GO:0007173"(epidermal growth factor receptor signaling pathway)|go:"GO:0009887"(animal organ morphogenesis)|go:"GO:0010628"(positive regulation of gene expression)|go:"GO:0010828"(positive regulation of glucose transmembrane transport)|go:"GO:0033138"(positive regulation of peptidyl-serine phosphorylation)|rcsb pdb:7M0T|rcsb pdb:7M0U|rcsb pdb:7M0V|go:"GO:0042802"(identical protein binding)|rcsb pdb:7M0W|rcsb pdb:7M0X|rcsb pdb:7M0Y|rcsb pdb:7M0Z|reactome:R-HSA-1295596|reactome:R-HSA-170968|reactome:R-HSA-170984|reactome:R-HSA-187706|reactome:R-HSA-5673000|reactome:R-HSA-5674135|reactome:R-HSA-5674499|reactome:R-HSA-5675221|reactome:R-HSA-6802946|reactome:R-HSA-6802948|reactome:R-HSA-6802952|reactome:R-HSA-6802955|reactome:R-HSA-9649948|reactome:R-HSA-9656223|reactome:R-HSA-9660537|rcsb pdb:4FK3|rcsb pdb:4G9C|rcsb pdb:4G9R|rcsb pdb:4H58|rcsb pdb:4JVG|rcsb pdb:4KSP|rcsb pdb:4KSQ|rcsb pdb:4FC0|rcsb pdb:4MBJ|rcsb pdb:4MNE|rcsb pdb:4MNF|rcsb pdb:4PP7|rcsb pdb:4R5Y|rcsb pdb:4RZV|rcsb pdb:4RZW|rcsb pdb:4WO5|rcsb pdb:4XV1|rcsb pdb:4XV2|rcsb pdb:4XV3|rcsb pdb:4XV9|rcsb pdb:4YHT|rcsb pdb:5C9C|rcsb pdb:5CSW|rcsb pdb:5CSX|rcsb pdb:5CT7|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0070374"(positive regulation of ERK1 and ERK2 cascade)|go:"GO:0043066"(negative regulation of apoptotic process)|go:"GO:0070413"(trehalose metabolism in response to stress)|go:"GO:0071277"(cellular response to calcium ion)|go:"GO:0090150"(establishment of protein localization to membrane)|go:"GO:0097110"(scaffold protein binding)|go:"GO:0106310"(protein serine kinase activity)|go:"GO:0106311"|interpro:IPR000719(Protein kinase, core)|interpro:IPR001245(Tyrosine protein kinase)|interpro:IPR002219(Protein kinase C, phorbol ester/diacylglycerol binding)|interpro:IPR003116(Raf-like Ras-binding)|interpro:IPR008271(Serine/threonine protein kinase, active site)|interpro:IPR011009(Protein kinase-like)|interpro:IPR017441(Protein kinase ATP binding, conserved site)|interpro:IPR020454|rcsb pdb:5FD2|rcsb pdb:5HI2|rcsb pdb:5HID|rcsb pdb:5HIE|rcsb pdb:5ITA|rcsb pdb:5J17|rcsb pdb:5J18|rcsb pdb:5J2R|rcsb pdb:5JRQ|rcsb pdb:5JSM|rcsb pdb:5JT2|rcsb pdb:5VAL|rcsb pdb:5VAM|rcsb pdb:5VR3|rcsb pdb:5VYK|rcsb pdb:6B8U|rcsb pdb:6CAD|rcsb pdb:6N0P|rcsb pdb:6N0Q|rcsb pdb:6NSQ|rcsb pdb:6NYB|rcsb pdb:6P3D|rcsb pdb:6P7G|rcsb pdb:6PP9|rcsb pdb:6Q0J|rcsb pdb:6Q0K|rcsb pdb:6Q0T|rcsb pdb:6U2G|rcsb pdb:6U2H|rcsb pdb:6UAN|rcsb pdb:6UUO|rcsb pdb:6V2U|rcsb pdb:6V2W|rcsb pdb:6XAG|mint:P15056|rcsb pdb:1UWH|rcsb pdb:1UWJ|rcsb pdb:2FB8|rcsb pdb:2L05|rcsb pdb:3C4C|interpro:IPR029071|rcsb pdb:3D4Q|rcsb pdb:3IDP|rcsb pdb:3II5|rcsb pdb:3NY5|rcsb pdb:3OG7|rcsb pdb:3PPJ|rcsb pdb:3PPK|rcsb pdb:3PRF|rcsb pdb:3PRI|rcsb pdb:3PSB|rcsb pdb:3PSD|rcsb pdb:3Q4C|rcsb pdb:3Q96|rcsb pdb:3SKC|rcsb pdb:3TV4|rcsb pdb:3TV6|rcsb pdb:4CQE|rcsb pdb:4DBN|rcsb pdb:4E26|rcsb pdb:4E4X|rcsb pdb:4EHE|rcsb pdb:4EHG|refseq:NP_004324.2|ensembl:ENSP00000493543|dip:DIP-1045N	-	-	-	figure legend:4 B/C, 6B|antagonist:The MEK inhibitor  PD0325901 reduced the level of MEK1 in complex with BRAF and ARAF.|dataset:Cancer - Interactions investigated in the context of cancer|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:9606(human-a375)|taxid:9606(Human malignant melanoma)	-	2014/06/12	2014/10/16	rogid:ieBLcDINn9hZFZZEIQ3Ktdf2NF89606	rogid:sfzILpNpX8UFB/vgH9LOKLpl/+g9606	intact-crc:9A1398B382DF44ED|rigid:VLl+5T+aNh8kZKnWh1WwvnrN0PI	false	-	-	-	-	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)
uniprotkb:Q02750	uniprotkb:P10398	intact:EBI-492564|ensembl:ENSP00000302486	intact:EBI-365961|uniprotkb:P07557|uniprotkb:Q5H9B3|intact:EBI-28988863|ensembl:ENSP00000366244|uniprotkb:Q5H9B2	psi-mi:mp2k1_human(display_long)|uniprotkb:ERK activator kinase 1(gene name synonym)|uniprotkb:MAPK/ERK kinase 1(gene name synonym)|uniprotkb:MAP2K1(gene name)|psi-mi:MAP2K1(display_short)|uniprotkb:MEK1(gene name synonym)|uniprotkb:PRKMK1(gene name synonym)	psi-mi:araf_human(display_long)|uniprotkb:ARAF(gene name)|psi-mi:ARAF(display_short)|uniprotkb:PKS(gene name synonym)|uniprotkb:PKS2(gene name synonym)|uniprotkb:ARAF1(gene name synonym)|uniprotkb:Proto-oncogene A-Raf-1(gene name synonym)|uniprotkb:Proto-oncogene Pks(gene name synonym)|uniprotkb:Proto-oncogene A-Raf(gene name synonym)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Lito et al. (2014)	pubmed:24746704|imex:IM-22983	taxid:9606(human)|taxid:9606(Homo sapiens)	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-9549280|imex:IM-22983-2	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_002746.1|refseq:XP_016877900.1|dip:DIP-201N|ensembl:ENSG00000169032(gene)|ensembl:ENST00000307102(transcript)|go:"GO:0000187"(obsolete activation of MAPK activity)|go:"GO:0004672"(protein kinase activity)|go:"GO:0004674"(protein serine/threonine kinase activity)|go:"GO:0004708"(MAP kinase kinase activity)|go:"GO:0004712"(protein serine/threonine/tyrosine kinase activity)|go:"GO:0004714"(transmembrane receptor protein tyrosine kinase activity)|go:"GO:0005078"(MAP-kinase scaffold activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005634"(nucleus)|go:"GO:0005739"(mitochondrion)|go:"GO:0005769"(early endosome)|go:"GO:0005770"(late endosome)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005815"(microtubule organizing center)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0005925"(focal adhesion)|go:"GO:0006468"(protein phosphorylation)|go:"GO:0006935"(chemotaxis)|go:"GO:0007165"(signal transduction)|go:"GO:0007507"(heart development)|go:"GO:0008022"(protein C-terminus binding)|go:"GO:0008285"(negative regulation of cell population proliferation)|go:"GO:0010628"(positive regulation of gene expression)|go:"GO:0018107"(peptidyl-threonine phosphorylation)|go:"GO:0021697"(cerebellar cortex formation)|go:"GO:0030182"(neuron differentiation)|go:"GO:0030216"(keratinocyte differentiation)|go:"GO:0030878"(thyroid gland development)|go:"GO:0032872"(regulation of stress-activated MAPK cascade)|go:"GO:0043539"(protein serine/threonine kinase activator activity)|go:"GO:0045893"(positive regulation of transcription, DNA-templated)|go:"GO:0047485"(protein N-terminus binding)|go:"GO:0048538"(thymus development)|go:"GO:0048679"(regulation of axon regeneration)|go:"GO:0048870"(cell motility)|go:"GO:0050772"(positive regulation of axonogenesis)|go:"GO:0051726"(regulation of cell cycle)|go:"GO:0060020"(Bergmann glial cell differentiation)|go:"GO:0060324"(face development)|go:"GO:0060440"(trachea formation)|go:"GO:0060502"(epithelial cell proliferation involved in lung morphogenesis)|go:"GO:0060674"(placenta blood vessel development)|go:"GO:0060711"(labyrinthine layer development)|go:"GO:0070371"(ERK1 and ERK2 cascade)|go:"GO:0070374"(positive regulation of ERK1 and ERK2 cascade)|go:"GO:0071902"(positive regulation of protein serine/threonine kinase activity)|go:"GO:0090170"(regulation of Golgi inheritance)|go:"GO:0090398"(cellular senescence)|go:"GO:0097110"(scaffold protein binding)|go:"GO:0106310"(protein serine kinase activity)|go:"GO:0106311"|go:"GO:1903800"(positive regulation of production of miRNAs involved in gene silencing by miRNA)|go:"GO:2000641"(regulation of early endosome to late endosome transport)|interpro:IPR000719(Protein kinase, core)|interpro:IPR008271(Serine/threonine protein kinase, active site)|interpro:IPR011009(Protein kinase-like)|interpro:IPR017441(Protein kinase ATP binding, conserved site)|mint:Q02750|rcsb pdb:1S9J|rcsb pdb:2P55|rcsb pdb:3DV3|rcsb pdb:3DY7|rcsb pdb:3E8N|rcsb pdb:3EQB|rcsb pdb:3EQC|rcsb pdb:3EQD|rcsb pdb:3EQF|rcsb pdb:3EQG|rcsb pdb:3EQH|rcsb pdb:3EQI|rcsb pdb:3MBL|rcsb pdb:3ORN|rcsb pdb:3OS3|rcsb pdb:3PP1|rcsb pdb:3SLS|rcsb pdb:3V01|rcsb pdb:3V04|rcsb pdb:3VVH|rcsb pdb:3W8Q|rcsb pdb:3WIG|rcsb pdb:3ZLS|rcsb pdb:3ZLW|rcsb pdb:3ZLX|rcsb pdb:3ZLY|rcsb pdb:3ZM4|rcsb pdb:4AN2|rcsb pdb:4AN3|rcsb pdb:4AN9|rcsb pdb:4ANB|rcsb pdb:4ARK|rcsb pdb:4LMN|rcsb pdb:4MNE|rcsb pdb:4U7Z|rcsb pdb:4U80|rcsb pdb:4U81|rcsb pdb:5EYM|rcsb pdb:5HZE|rcsb pdb:5YT3|rcsb pdb:6NYB|rcsb pdb:6PP9|rcsb pdb:6Q0J|rcsb pdb:6Q0T|rcsb pdb:6U2G|rcsb pdb:6V2W|rcsb pdb:6X2P|rcsb pdb:6X2S|rcsb pdb:6X2X|rcsb pdb:7B3M|rcsb pdb:7B7R|rcsb pdb:7B94|rcsb pdb:7B9L|rcsb pdb:7M0T|rcsb pdb:7M0U|rcsb pdb:7M0V|rcsb pdb:7M0W|rcsb pdb:7M0X|rcsb pdb:7M0Y|rcsb pdb:7M0Z|reactome:R-HSA-110056|reactome:R-HSA-170968|reactome:R-HSA-445144|reactome:R-HSA-5210891|reactome:R-HSA-5673000|reactome:R-HSA-5674135|reactome:R-HSA-5674499|reactome:R-HSA-5684264|reactome:R-HSA-6802946|reactome:R-HSA-6802948|reactome:R-HSA-6802952|reactome:R-HSA-6802955|reactome:R-HSA-9649948|reactome:R-HSA-9652169|reactome:R-HSA-9656223|rcsb pdb:5BX0	ensembl:ENSG00000078061(gene)|ensembl:ENST00000377045(transcript)|go:"GO:0004672"(protein kinase activity)|go:"GO:0004674"(protein serine/threonine kinase activity)|go:"GO:0004709"(MAP kinase kinase kinase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005739"(mitochondrion)|go:"GO:0005829"(cytosol)|go:"GO:0006464"(cellular protein modification process)|go:"GO:0032006"(regulation of TOR signaling)|go:"GO:0032434"(regulation of proteasomal ubiquitin-dependent protein catabolic process)|go:"GO:0033138"(positive regulation of peptidyl-serine phosphorylation)|go:"GO:0043066"(negative regulation of apoptotic process)|go:"GO:0046872"(metal ion binding)|go:"GO:0106310"(protein serine kinase activity)|go:"GO:0106311"|interpro:IPR000719(Protein kinase, core)|interpro:IPR001245(Tyrosine protein kinase)|interpro:IPR002219(Protein kinase C, phorbol ester/diacylglycerol binding)|interpro:IPR003116(Raf-like Ras-binding)|interpro:IPR008271(Serine/threonine protein kinase, active site)|interpro:IPR011009(Protein kinase-like)|interpro:IPR017441(Protein kinase ATP binding, conserved site)|interpro:IPR020454|interpro:IPR029071|mint:P10398|rcsb pdb:2MSE|reactome:R-HSA-5673000|reactome:R-HSA-5674135|reactome:R-HSA-5675221|reactome:R-HSA-6802946|reactome:R-HSA-6802948|reactome:R-HSA-6802952|reactome:R-HSA-6802955|reactome:R-HSA-9649948|reactome:R-HSA-9656223|reactome:R-HSA-9660537|rcsb pdb:1WXM|refseq:NP_001645.1|refseq:XP_011542210.1|refseq:NP_001243125.1|ensembl:ENST00000377039|ensembl:ENSP00000366238|ensembl:ENSP00000366244|refseq:NP_001243126.1|dip:DIP-31374N	-	-	-	figure legend:4 B/C, 6B|antagonist:The MEK inhibitor  PD0325901 reduced the level of MEK1 in complex with BRAF and ARAF.|dataset:Cancer - Interactions investigated in the context of cancer|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:9606(human-a375)|taxid:9606(Human malignant melanoma)	-	2014/06/12	2014/10/16	rogid:ieBLcDINn9hZFZZEIQ3Ktdf2NF89606	rogid:WRtbdrEuDxNkA6jRRxvapodFcUE9606	intact-crc:9A1398B382DF44ED|rigid:VLl+5T+aNh8kZKnWh1WwvnrN0PI	false	-	-	-	-	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)
uniprotkb:Q02750	uniprotkb:P04049	intact:EBI-492564|ensembl:ENSP00000302486	intact:EBI-365996|uniprotkb:B0LPH8|uniprotkb:Q15278|uniprotkb:Q9UC20|ensembl:ENSP00000251849|uniprotkb:B2R5N3	psi-mi:mp2k1_human(display_long)|uniprotkb:ERK activator kinase 1(gene name synonym)|uniprotkb:MAPK/ERK kinase 1(gene name synonym)|uniprotkb:MAP2K1(gene name)|psi-mi:MAP2K1(display_short)|uniprotkb:MEK1(gene name synonym)|uniprotkb:PRKMK1(gene name synonym)	psi-mi:raf1_human(display_long)|uniprotkb:RAF1(gene name)|psi-mi:RAF1(display_short)|uniprotkb:RAF(gene name synonym)|uniprotkb:Proto-oncogene c-RAF(gene name synonym)|uniprotkb:Raf-1(gene name synonym)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Lito et al. (2014)	pubmed:24746704|imex:IM-22983	taxid:9606(human)|taxid:9606(Homo sapiens)	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-9549280|imex:IM-22983-2	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_002746.1|refseq:XP_016877900.1|dip:DIP-201N|ensembl:ENSG00000169032(gene)|ensembl:ENST00000307102(transcript)|go:"GO:0000187"(obsolete activation of MAPK activity)|go:"GO:0004672"(protein kinase activity)|go:"GO:0004674"(protein serine/threonine kinase activity)|go:"GO:0004708"(MAP kinase kinase activity)|go:"GO:0004712"(protein serine/threonine/tyrosine kinase activity)|go:"GO:0004714"(transmembrane receptor protein tyrosine kinase activity)|go:"GO:0005078"(MAP-kinase scaffold activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005634"(nucleus)|go:"GO:0005739"(mitochondrion)|go:"GO:0005769"(early endosome)|go:"GO:0005770"(late endosome)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005815"(microtubule organizing center)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0005925"(focal adhesion)|go:"GO:0006468"(protein phosphorylation)|go:"GO:0006935"(chemotaxis)|go:"GO:0007165"(signal transduction)|go:"GO:0007507"(heart development)|go:"GO:0008022"(protein C-terminus binding)|go:"GO:0008285"(negative regulation of cell population proliferation)|go:"GO:0010628"(positive regulation of gene expression)|go:"GO:0018107"(peptidyl-threonine phosphorylation)|go:"GO:0021697"(cerebellar cortex formation)|go:"GO:0030182"(neuron differentiation)|go:"GO:0030216"(keratinocyte differentiation)|go:"GO:0030878"(thyroid gland development)|go:"GO:0032872"(regulation of stress-activated MAPK cascade)|go:"GO:0043539"(protein serine/threonine kinase activator activity)|go:"GO:0045893"(positive regulation of transcription, DNA-templated)|go:"GO:0047485"(protein N-terminus binding)|go:"GO:0048538"(thymus development)|go:"GO:0048679"(regulation of axon regeneration)|go:"GO:0048870"(cell motility)|go:"GO:0050772"(positive regulation of axonogenesis)|go:"GO:0051726"(regulation of cell cycle)|go:"GO:0060020"(Bergmann glial cell differentiation)|go:"GO:0060324"(face development)|go:"GO:0060440"(trachea formation)|go:"GO:0060502"(epithelial cell proliferation involved in lung morphogenesis)|go:"GO:0060674"(placenta blood vessel development)|go:"GO:0060711"(labyrinthine layer development)|go:"GO:0070371"(ERK1 and ERK2 cascade)|go:"GO:0070374"(positive regulation of ERK1 and ERK2 cascade)|go:"GO:0071902"(positive regulation of protein serine/threonine kinase activity)|go:"GO:0090170"(regulation of Golgi inheritance)|go:"GO:0090398"(cellular senescence)|go:"GO:0097110"(scaffold protein binding)|go:"GO:0106310"(protein serine kinase activity)|go:"GO:0106311"|go:"GO:1903800"(positive regulation of production of miRNAs involved in gene silencing by miRNA)|go:"GO:2000641"(regulation of early endosome to late endosome transport)|interpro:IPR000719(Protein kinase, core)|interpro:IPR008271(Serine/threonine protein kinase, active site)|interpro:IPR011009(Protein kinase-like)|interpro:IPR017441(Protein kinase ATP binding, conserved site)|mint:Q02750|rcsb pdb:1S9J|rcsb pdb:2P55|rcsb pdb:3DV3|rcsb pdb:3DY7|rcsb pdb:3E8N|rcsb pdb:3EQB|rcsb pdb:3EQC|rcsb pdb:3EQD|rcsb pdb:3EQF|rcsb pdb:3EQG|rcsb pdb:3EQH|rcsb pdb:3EQI|rcsb pdb:3MBL|rcsb pdb:3ORN|rcsb pdb:3OS3|rcsb pdb:3PP1|rcsb pdb:3SLS|rcsb pdb:3V01|rcsb pdb:3V04|rcsb pdb:3VVH|rcsb pdb:3W8Q|rcsb pdb:3WIG|rcsb pdb:3ZLS|rcsb pdb:3ZLW|rcsb pdb:3ZLX|rcsb pdb:3ZLY|rcsb pdb:3ZM4|rcsb pdb:4AN2|rcsb pdb:4AN3|rcsb pdb:4AN9|rcsb pdb:4ANB|rcsb pdb:4ARK|rcsb pdb:4LMN|rcsb pdb:4MNE|rcsb pdb:4U7Z|rcsb pdb:4U80|rcsb pdb:4U81|rcsb pdb:5EYM|rcsb pdb:5HZE|rcsb pdb:5YT3|rcsb pdb:6NYB|rcsb pdb:6PP9|rcsb pdb:6Q0J|rcsb pdb:6Q0T|rcsb pdb:6U2G|rcsb pdb:6V2W|rcsb pdb:6X2P|rcsb pdb:6X2S|rcsb pdb:6X2X|rcsb pdb:7B3M|rcsb pdb:7B7R|rcsb pdb:7B94|rcsb pdb:7B9L|rcsb pdb:7M0T|rcsb pdb:7M0U|rcsb pdb:7M0V|rcsb pdb:7M0W|rcsb pdb:7M0X|rcsb pdb:7M0Y|rcsb pdb:7M0Z|reactome:R-HSA-110056|reactome:R-HSA-170968|reactome:R-HSA-445144|reactome:R-HSA-5210891|reactome:R-HSA-5673000|reactome:R-HSA-5674135|reactome:R-HSA-5674499|reactome:R-HSA-5684264|reactome:R-HSA-6802946|reactome:R-HSA-6802948|reactome:R-HSA-6802952|reactome:R-HSA-6802955|reactome:R-HSA-9649948|reactome:R-HSA-9652169|reactome:R-HSA-9656223|rcsb pdb:5BX0	rcsb pdb:3IQV|rcsb pdb:3KUC|rcsb pdb:3KUD|rcsb pdb:3NKX|rcsb pdb:3O8I|rcsb pdb:3OMV|rcsb pdb:4FJ3|rcsb pdb:4G0N|rcsb pdb:4G3X|rcsb pdb:4IEA|rcsb pdb:4IHL|rcsb pdb:6NTC|rcsb pdb:6NTD|rcsb pdb:6PTS|rcsb pdb:6PTW|rcsb pdb:6VJJ|rcsb pdb:6XGU|rcsb pdb:6XGV|rcsb pdb:6XHA|rcsb pdb:6XHB|rcsb pdb:6XI7|ensembl:ENSG00000132155(gene)|ensembl:ENST00000251849(transcript)|go:"GO:0000186"(obsolete activation of MAPKK activity)|go:"GO:0001666"(response to hypoxia)|go:"GO:0004672"(protein kinase activity)|go:"GO:0004674"(protein serine/threonine kinase activity)|go:"GO:0004709"(MAP kinase kinase kinase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005737"(cytoplasm)|go:"GO:0005739"(mitochondrion)|go:"GO:0005741"(mitochondrial outer membrane)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0006468"(protein phosphorylation)|go:"GO:0006915"(apoptotic process)|go:"GO:0007165"(signal transduction)|go:"GO:0007190"(activation of adenylate cyclase activity)|go:"GO:0007507"(heart development)|go:"GO:0008179"(adenylate cyclase binding)|go:"GO:0008285"(negative regulation of cell population proliferation)|go:"GO:0010856"(adenylate cyclase activator activity)|go:"GO:0016607"(nuclear speck)|go:"GO:0019899"(enzyme binding)|go:"GO:0030154"(cell differentiation)|go:"GO:0030878"(thyroid gland development)|go:"GO:0031143"(pseudopodium)|go:"GO:0031333"(negative regulation of protein-containing complex assembly)|go:"GO:0031434"(mitogen-activated protein kinase kinase binding)|go:"GO:0033138"(positive regulation of peptidyl-serine phosphorylation)|go:"GO:0035019"(somatic stem cell population maintenance)|go:"GO:0035023"(regulation of Rho protein signal transduction)|go:"GO:0035773"(insulin secretion involved in cellular response to glucose stimulus)|go:"GO:0035994"(response to muscle stretch)|go:"GO:0042060"(wound healing)|go:"GO:0042802"(identical protein binding)|go:"GO:0042981"(regulation of apoptotic process)|go:"GO:0043066"(negative regulation of apoptotic process)|go:"GO:0043154"(negative regulation of cysteine-type endopeptidase activity involved in apoptotic process)|go:"GO:0044877"(protein-containing complex binding)|go:"GO:0045104"(intermediate filament cytoskeleton organization)|go:"GO:0045595"(regulation of cell differentiation)|go:"GO:0045944"(positive regulation of transcription by RNA polymerase II)|go:"GO:0046872"(metal ion binding)|go:"GO:0048011"(neurotrophin TRK receptor signaling pathway)|go:"GO:0048538"(thymus development)|go:"GO:0060324"(face development)|go:"GO:0071550"(death-inducing signaling complex assembly)|go:"GO:0106310"(protein serine kinase activity)|go:"GO:0106311"|go:"GO:1902042"(negative regulation of extrinsic apoptotic signaling pathway via death domain receptors)|go:"GO:2000145"(regulation of cell motility)|interpro:IPR000719(Protein kinase, core)|interpro:IPR001245(Tyrosine protein kinase)|interpro:IPR002219(Protein kinase C, phorbol ester/diacylglycerol binding)|interpro:IPR003116(Raf-like Ras-binding)|interpro:IPR008271(Serine/threonine protein kinase, active site)|interpro:IPR011009(Protein kinase-like)|interpro:IPR017441(Protein kinase ATP binding, conserved site)|interpro:IPR020454|interpro:IPR029071|mint:P04049|rcsb pdb:1C1Y|rcsb pdb:1FAQ|rcsb pdb:1FAR|rcsb pdb:1GUA|rcsb pdb:1RFA|rcsb pdb:3CU8|rcsb pdb:3IQJ|rcsb pdb:3IQU|rcsb pdb:7JHP|reactome:R-HSA-2672351|reactome:R-HSA-392517|reactome:R-HSA-430116|reactome:R-HSA-5621575|reactome:R-HSA-5673000|reactome:R-HSA-5674135|reactome:R-HSA-5674499|reactome:R-HSA-5675221|reactome:R-HSA-6802946|reactome:R-HSA-6802948|reactome:R-HSA-6802952|reactome:R-HSA-6802955|reactome:R-HSA-9649948|reactome:R-HSA-9656223|reactome:R-HSA-9660537|refseq:NP_002871.1|refseq:XP_011532276.1|refseq:XP_005265412.1|dip:DIP-1048N	-	-	-	figure legend:4 B/C, 6B|antagonist:The MEK inhibitor  PD0325901 reduced the level of MEK1 in complex with BRAF and ARAF.|dataset:Cancer - Interactions investigated in the context of cancer|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:9606(human-a375)|taxid:9606(Human malignant melanoma)	-	2014/06/12	2014/10/16	rogid:ieBLcDINn9hZFZZEIQ3Ktdf2NF89606	rogid:1WOAPuzcZdMoq9hyFD/qr4Tz7049606	intact-crc:9A1398B382DF44ED|rigid:VLl+5T+aNh8kZKnWh1WwvnrN0PI	false	-	-	-	-	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)
uniprotkb:Q02750	uniprotkb:P15056	intact:EBI-492564|ensembl:ENSP00000302486	intact:EBI-365980|uniprotkb:Q13878|uniprotkb:Q3MIN6|intact:EBI-28988924|ensembl:ENSP00000493543|uniprotkb:Q9UDP8|uniprotkb:Q9Y6T3|uniprotkb:A4D1T4|uniprotkb:B6HY61|uniprotkb:B6HY62|uniprotkb:B6HY63|uniprotkb:B6HY64|uniprotkb:B6HY65|uniprotkb:B6HY66	psi-mi:mp2k1_human(display_long)|uniprotkb:ERK activator kinase 1(gene name synonym)|uniprotkb:MAPK/ERK kinase 1(gene name synonym)|uniprotkb:MAP2K1(gene name)|psi-mi:MAP2K1(display_short)|uniprotkb:MEK1(gene name synonym)|uniprotkb:PRKMK1(gene name synonym)	psi-mi:braf_human(display_long)|uniprotkb:BRAF(gene name)|psi-mi:BRAF(display_short)|uniprotkb:BRAF1(gene name synonym)|uniprotkb:RAFB1(gene name synonym)|uniprotkb:v-Raf murine sarcoma viral oncogene homolog B1(gene name synonym)|uniprotkb:p94(gene name synonym)|uniprotkb:Proto-oncogene B-Raf(gene name synonym)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Lito et al. (2014)	pubmed:24746704|imex:IM-22983	taxid:9606(human)|taxid:9606(Homo sapiens)	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-9549313|imex:IM-22983-17	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_002746.1|refseq:XP_016877900.1|dip:DIP-201N|ensembl:ENSG00000169032(gene)|ensembl:ENST00000307102(transcript)|go:"GO:0000187"(obsolete activation of MAPK activity)|go:"GO:0004672"(protein kinase activity)|go:"GO:0004674"(protein serine/threonine kinase activity)|go:"GO:0004708"(MAP kinase kinase activity)|go:"GO:0004712"(protein serine/threonine/tyrosine kinase activity)|go:"GO:0004714"(transmembrane receptor protein tyrosine kinase activity)|go:"GO:0005078"(MAP-kinase scaffold activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005634"(nucleus)|go:"GO:0005739"(mitochondrion)|go:"GO:0005769"(early endosome)|go:"GO:0005770"(late endosome)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005815"(microtubule organizing center)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0005925"(focal adhesion)|go:"GO:0006468"(protein phosphorylation)|go:"GO:0006935"(chemotaxis)|go:"GO:0007165"(signal transduction)|go:"GO:0007507"(heart development)|go:"GO:0008022"(protein C-terminus binding)|go:"GO:0008285"(negative regulation of cell population proliferation)|go:"GO:0010628"(positive regulation of gene expression)|go:"GO:0018107"(peptidyl-threonine phosphorylation)|go:"GO:0021697"(cerebellar cortex formation)|go:"GO:0030182"(neuron differentiation)|go:"GO:0030216"(keratinocyte differentiation)|go:"GO:0030878"(thyroid gland development)|go:"GO:0032872"(regulation of stress-activated MAPK cascade)|go:"GO:0043539"(protein serine/threonine kinase activator activity)|go:"GO:0045893"(positive regulation of transcription, DNA-templated)|go:"GO:0047485"(protein N-terminus binding)|go:"GO:0048538"(thymus development)|go:"GO:0048679"(regulation of axon regeneration)|go:"GO:0048870"(cell motility)|go:"GO:0050772"(positive regulation of axonogenesis)|go:"GO:0051726"(regulation of cell cycle)|go:"GO:0060020"(Bergmann glial cell differentiation)|go:"GO:0060324"(face development)|go:"GO:0060440"(trachea formation)|go:"GO:0060502"(epithelial cell proliferation involved in lung morphogenesis)|go:"GO:0060674"(placenta blood vessel development)|go:"GO:0060711"(labyrinthine layer development)|go:"GO:0070371"(ERK1 and ERK2 cascade)|go:"GO:0070374"(positive regulation of ERK1 and ERK2 cascade)|go:"GO:0071902"(positive regulation of protein serine/threonine kinase activity)|go:"GO:0090170"(regulation of Golgi inheritance)|go:"GO:0090398"(cellular senescence)|go:"GO:0097110"(scaffold protein binding)|go:"GO:0106310"(protein serine kinase activity)|go:"GO:0106311"|go:"GO:1903800"(positive regulation of production of miRNAs involved in gene silencing by miRNA)|go:"GO:2000641"(regulation of early endosome to late endosome transport)|interpro:IPR000719(Protein kinase, core)|interpro:IPR008271(Serine/threonine protein kinase, active site)|interpro:IPR011009(Protein kinase-like)|interpro:IPR017441(Protein kinase ATP binding, conserved site)|mint:Q02750|rcsb pdb:1S9J|rcsb pdb:2P55|rcsb pdb:3DV3|rcsb pdb:3DY7|rcsb pdb:3E8N|rcsb pdb:3EQB|rcsb pdb:3EQC|rcsb pdb:3EQD|rcsb pdb:3EQF|rcsb pdb:3EQG|rcsb pdb:3EQH|rcsb pdb:3EQI|rcsb pdb:3MBL|rcsb pdb:3ORN|rcsb pdb:3OS3|rcsb pdb:3PP1|rcsb pdb:3SLS|rcsb pdb:3V01|rcsb pdb:3V04|rcsb pdb:3VVH|rcsb pdb:3W8Q|rcsb pdb:3WIG|rcsb pdb:3ZLS|rcsb pdb:3ZLW|rcsb pdb:3ZLX|rcsb pdb:3ZLY|rcsb pdb:3ZM4|rcsb pdb:4AN2|rcsb pdb:4AN3|rcsb pdb:4AN9|rcsb pdb:4ANB|rcsb pdb:4ARK|rcsb pdb:4LMN|rcsb pdb:4MNE|rcsb pdb:4U7Z|rcsb pdb:4U80|rcsb pdb:4U81|rcsb pdb:5EYM|rcsb pdb:5HZE|rcsb pdb:5YT3|rcsb pdb:6NYB|rcsb pdb:6PP9|rcsb pdb:6Q0J|rcsb pdb:6Q0T|rcsb pdb:6U2G|rcsb pdb:6V2W|rcsb pdb:6X2P|rcsb pdb:6X2S|rcsb pdb:6X2X|rcsb pdb:7B3M|rcsb pdb:7B7R|rcsb pdb:7B94|rcsb pdb:7B9L|rcsb pdb:7M0T|rcsb pdb:7M0U|rcsb pdb:7M0V|rcsb pdb:7M0W|rcsb pdb:7M0X|rcsb pdb:7M0Y|rcsb pdb:7M0Z|reactome:R-HSA-110056|reactome:R-HSA-170968|reactome:R-HSA-445144|reactome:R-HSA-5210891|reactome:R-HSA-5673000|reactome:R-HSA-5674135|reactome:R-HSA-5674499|reactome:R-HSA-5684264|reactome:R-HSA-6802946|reactome:R-HSA-6802948|reactome:R-HSA-6802952|reactome:R-HSA-6802955|reactome:R-HSA-9649948|reactome:R-HSA-9652169|reactome:R-HSA-9656223|rcsb pdb:5BX0	ensembl:ENSG00000157764(gene)|ensembl:ENST00000646891(transcript)|go:"GO:0000165"(MAPK cascade)|go:"GO:0004672"(protein kinase activity)|go:"GO:0004674"(protein serine/threonine kinase activity)|go:"GO:0004708"(MAP kinase kinase activity)|go:"GO:0005509"(calcium ion binding)|go:"GO:0005524"(ATP binding)|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0006468"(protein phosphorylation)|go:"GO:0007173"(epidermal growth factor receptor signaling pathway)|go:"GO:0009887"(animal organ morphogenesis)|go:"GO:0010628"(positive regulation of gene expression)|go:"GO:0010828"(positive regulation of glucose transmembrane transport)|go:"GO:0033138"(positive regulation of peptidyl-serine phosphorylation)|rcsb pdb:7M0T|rcsb pdb:7M0U|rcsb pdb:7M0V|go:"GO:0042802"(identical protein binding)|rcsb pdb:7M0W|rcsb pdb:7M0X|rcsb pdb:7M0Y|rcsb pdb:7M0Z|reactome:R-HSA-1295596|reactome:R-HSA-170968|reactome:R-HSA-170984|reactome:R-HSA-187706|reactome:R-HSA-5673000|reactome:R-HSA-5674135|reactome:R-HSA-5674499|reactome:R-HSA-5675221|reactome:R-HSA-6802946|reactome:R-HSA-6802948|reactome:R-HSA-6802952|reactome:R-HSA-6802955|reactome:R-HSA-9649948|reactome:R-HSA-9656223|reactome:R-HSA-9660537|rcsb pdb:4FK3|rcsb pdb:4G9C|rcsb pdb:4G9R|rcsb pdb:4H58|rcsb pdb:4JVG|rcsb pdb:4KSP|rcsb pdb:4KSQ|rcsb pdb:4FC0|rcsb pdb:4MBJ|rcsb pdb:4MNE|rcsb pdb:4MNF|rcsb pdb:4PP7|rcsb pdb:4R5Y|rcsb pdb:4RZV|rcsb pdb:4RZW|rcsb pdb:4WO5|rcsb pdb:4XV1|rcsb pdb:4XV2|rcsb pdb:4XV3|rcsb pdb:4XV9|rcsb pdb:4YHT|rcsb pdb:5C9C|rcsb pdb:5CSW|rcsb pdb:5CSX|rcsb pdb:5CT7|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0070374"(positive regulation of ERK1 and ERK2 cascade)|go:"GO:0043066"(negative regulation of apoptotic process)|go:"GO:0070413"(trehalose metabolism in response to stress)|go:"GO:0071277"(cellular response to calcium ion)|go:"GO:0090150"(establishment of protein localization to membrane)|go:"GO:0097110"(scaffold protein binding)|go:"GO:0106310"(protein serine kinase activity)|go:"GO:0106311"|interpro:IPR000719(Protein kinase, core)|interpro:IPR001245(Tyrosine protein kinase)|interpro:IPR002219(Protein kinase C, phorbol ester/diacylglycerol binding)|interpro:IPR003116(Raf-like Ras-binding)|interpro:IPR008271(Serine/threonine protein kinase, active site)|interpro:IPR011009(Protein kinase-like)|interpro:IPR017441(Protein kinase ATP binding, conserved site)|interpro:IPR020454|rcsb pdb:5FD2|rcsb pdb:5HI2|rcsb pdb:5HID|rcsb pdb:5HIE|rcsb pdb:5ITA|rcsb pdb:5J17|rcsb pdb:5J18|rcsb pdb:5J2R|rcsb pdb:5JRQ|rcsb pdb:5JSM|rcsb pdb:5JT2|rcsb pdb:5VAL|rcsb pdb:5VAM|rcsb pdb:5VR3|rcsb pdb:5VYK|rcsb pdb:6B8U|rcsb pdb:6CAD|rcsb pdb:6N0P|rcsb pdb:6N0Q|rcsb pdb:6NSQ|rcsb pdb:6NYB|rcsb pdb:6P3D|rcsb pdb:6P7G|rcsb pdb:6PP9|rcsb pdb:6Q0J|rcsb pdb:6Q0K|rcsb pdb:6Q0T|rcsb pdb:6U2G|rcsb pdb:6U2H|rcsb pdb:6UAN|rcsb pdb:6UUO|rcsb pdb:6V2U|rcsb pdb:6V2W|rcsb pdb:6XAG|mint:P15056|rcsb pdb:1UWH|rcsb pdb:1UWJ|rcsb pdb:2FB8|rcsb pdb:2L05|rcsb pdb:3C4C|interpro:IPR029071|rcsb pdb:3D4Q|rcsb pdb:3IDP|rcsb pdb:3II5|rcsb pdb:3NY5|rcsb pdb:3OG7|rcsb pdb:3PPJ|rcsb pdb:3PPK|rcsb pdb:3PRF|rcsb pdb:3PRI|rcsb pdb:3PSB|rcsb pdb:3PSD|rcsb pdb:3Q4C|rcsb pdb:3Q96|rcsb pdb:3SKC|rcsb pdb:3TV4|rcsb pdb:3TV6|rcsb pdb:4CQE|rcsb pdb:4DBN|rcsb pdb:4E26|rcsb pdb:4E4X|rcsb pdb:4EHE|rcsb pdb:4EHG|refseq:NP_004324.2|ensembl:ENSP00000493543|dip:DIP-1045N	-	-	-	figure legend:4 C, 6C|agonist:The MEK inhibitor  PD0325901|antagonist:Trametinib - a newer MEK inhibitor|dataset:Cancer - Interactions investigated in the context of cancer|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:9606(human-a_549)|taxid:9606(Homo sapiens Caucasian lung carcinoma cell line)	-	2014/06/12	2014/10/16	rogid:ieBLcDINn9hZFZZEIQ3Ktdf2NF89606	rogid:sfzILpNpX8UFB/vgH9LOKLpl/+g9606	intact-crc:21E9E6BE3E83B70A|rigid:VLl+5T+aNh8kZKnWh1WwvnrN0PI	false	-	-	-	-	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)
uniprotkb:Q02750	uniprotkb:P04049	intact:EBI-492564|ensembl:ENSP00000302486	intact:EBI-365996|uniprotkb:B0LPH8|uniprotkb:Q15278|uniprotkb:Q9UC20|ensembl:ENSP00000251849|uniprotkb:B2R5N3	psi-mi:mp2k1_human(display_long)|uniprotkb:ERK activator kinase 1(gene name synonym)|uniprotkb:MAPK/ERK kinase 1(gene name synonym)|uniprotkb:MAP2K1(gene name)|psi-mi:MAP2K1(display_short)|uniprotkb:MEK1(gene name synonym)|uniprotkb:PRKMK1(gene name synonym)	psi-mi:raf1_human(display_long)|uniprotkb:RAF1(gene name)|psi-mi:RAF1(display_short)|uniprotkb:RAF(gene name synonym)|uniprotkb:Proto-oncogene c-RAF(gene name synonym)|uniprotkb:Raf-1(gene name synonym)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Lito et al. (2014)	pubmed:24746704|imex:IM-22983	taxid:9606(human)|taxid:9606(Homo sapiens)	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-9549313|imex:IM-22983-17	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_002746.1|refseq:XP_016877900.1|dip:DIP-201N|ensembl:ENSG00000169032(gene)|ensembl:ENST00000307102(transcript)|go:"GO:0000187"(obsolete activation of MAPK activity)|go:"GO:0004672"(protein kinase activity)|go:"GO:0004674"(protein serine/threonine kinase activity)|go:"GO:0004708"(MAP kinase kinase activity)|go:"GO:0004712"(protein serine/threonine/tyrosine kinase activity)|go:"GO:0004714"(transmembrane receptor protein tyrosine kinase activity)|go:"GO:0005078"(MAP-kinase scaffold activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005634"(nucleus)|go:"GO:0005739"(mitochondrion)|go:"GO:0005769"(early endosome)|go:"GO:0005770"(late endosome)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005815"(microtubule organizing center)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0005925"(focal adhesion)|go:"GO:0006468"(protein phosphorylation)|go:"GO:0006935"(chemotaxis)|go:"GO:0007165"(signal transduction)|go:"GO:0007507"(heart development)|go:"GO:0008022"(protein C-terminus binding)|go:"GO:0008285"(negative regulation of cell population proliferation)|go:"GO:0010628"(positive regulation of gene expression)|go:"GO:0018107"(peptidyl-threonine phosphorylation)|go:"GO:0021697"(cerebellar cortex formation)|go:"GO:0030182"(neuron differentiation)|go:"GO:0030216"(keratinocyte differentiation)|go:"GO:0030878"(thyroid gland development)|go:"GO:0032872"(regulation of stress-activated MAPK cascade)|go:"GO:0043539"(protein serine/threonine kinase activator activity)|go:"GO:0045893"(positive regulation of transcription, DNA-templated)|go:"GO:0047485"(protein N-terminus binding)|go:"GO:0048538"(thymus development)|go:"GO:0048679"(regulation of axon regeneration)|go:"GO:0048870"(cell motility)|go:"GO:0050772"(positive regulation of axonogenesis)|go:"GO:0051726"(regulation of cell cycle)|go:"GO:0060020"(Bergmann glial cell differentiation)|go:"GO:0060324"(face development)|go:"GO:0060440"(trachea formation)|go:"GO:0060502"(epithelial cell proliferation involved in lung morphogenesis)|go:"GO:0060674"(placenta blood vessel development)|go:"GO:0060711"(labyrinthine layer development)|go:"GO:0070371"(ERK1 and ERK2 cascade)|go:"GO:0070374"(positive regulation of ERK1 and ERK2 cascade)|go:"GO:0071902"(positive regulation of protein serine/threonine kinase activity)|go:"GO:0090170"(regulation of Golgi inheritance)|go:"GO:0090398"(cellular senescence)|go:"GO:0097110"(scaffold protein binding)|go:"GO:0106310"(protein serine kinase activity)|go:"GO:0106311"|go:"GO:1903800"(positive regulation of production of miRNAs involved in gene silencing by miRNA)|go:"GO:2000641"(regulation of early endosome to late endosome transport)|interpro:IPR000719(Protein kinase, core)|interpro:IPR008271(Serine/threonine protein kinase, active site)|interpro:IPR011009(Protein kinase-like)|interpro:IPR017441(Protein kinase ATP binding, conserved site)|mint:Q02750|rcsb pdb:1S9J|rcsb pdb:2P55|rcsb pdb:3DV3|rcsb pdb:3DY7|rcsb pdb:3E8N|rcsb pdb:3EQB|rcsb pdb:3EQC|rcsb pdb:3EQD|rcsb pdb:3EQF|rcsb pdb:3EQG|rcsb pdb:3EQH|rcsb pdb:3EQI|rcsb pdb:3MBL|rcsb pdb:3ORN|rcsb pdb:3OS3|rcsb pdb:3PP1|rcsb pdb:3SLS|rcsb pdb:3V01|rcsb pdb:3V04|rcsb pdb:3VVH|rcsb pdb:3W8Q|rcsb pdb:3WIG|rcsb pdb:3ZLS|rcsb pdb:3ZLW|rcsb pdb:3ZLX|rcsb pdb:3ZLY|rcsb pdb:3ZM4|rcsb pdb:4AN2|rcsb pdb:4AN3|rcsb pdb:4AN9|rcsb pdb:4ANB|rcsb pdb:4ARK|rcsb pdb:4LMN|rcsb pdb:4MNE|rcsb pdb:4U7Z|rcsb pdb:4U80|rcsb pdb:4U81|rcsb pdb:5EYM|rcsb pdb:5HZE|rcsb pdb:5YT3|rcsb pdb:6NYB|rcsb pdb:6PP9|rcsb pdb:6Q0J|rcsb pdb:6Q0T|rcsb pdb:6U2G|rcsb pdb:6V2W|rcsb pdb:6X2P|rcsb pdb:6X2S|rcsb pdb:6X2X|rcsb pdb:7B3M|rcsb pdb:7B7R|rcsb pdb:7B94|rcsb pdb:7B9L|rcsb pdb:7M0T|rcsb pdb:7M0U|rcsb pdb:7M0V|rcsb pdb:7M0W|rcsb pdb:7M0X|rcsb pdb:7M0Y|rcsb pdb:7M0Z|reactome:R-HSA-110056|reactome:R-HSA-170968|reactome:R-HSA-445144|reactome:R-HSA-5210891|reactome:R-HSA-5673000|reactome:R-HSA-5674135|reactome:R-HSA-5674499|reactome:R-HSA-5684264|reactome:R-HSA-6802946|reactome:R-HSA-6802948|reactome:R-HSA-6802952|reactome:R-HSA-6802955|reactome:R-HSA-9649948|reactome:R-HSA-9652169|reactome:R-HSA-9656223|rcsb pdb:5BX0	rcsb pdb:3IQV|rcsb pdb:3KUC|rcsb pdb:3KUD|rcsb pdb:3NKX|rcsb pdb:3O8I|rcsb pdb:3OMV|rcsb pdb:4FJ3|rcsb pdb:4G0N|rcsb pdb:4G3X|rcsb pdb:4IEA|rcsb pdb:4IHL|rcsb pdb:6NTC|rcsb pdb:6NTD|rcsb pdb:6PTS|rcsb pdb:6PTW|rcsb pdb:6VJJ|rcsb pdb:6XGU|rcsb pdb:6XGV|rcsb pdb:6XHA|rcsb pdb:6XHB|rcsb pdb:6XI7|ensembl:ENSG00000132155(gene)|ensembl:ENST00000251849(transcript)|go:"GO:0000186"(obsolete activation of MAPKK activity)|go:"GO:0001666"(response to hypoxia)|go:"GO:0004672"(protein kinase activity)|go:"GO:0004674"(protein serine/threonine kinase activity)|go:"GO:0004709"(MAP kinase kinase kinase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005737"(cytoplasm)|go:"GO:0005739"(mitochondrion)|go:"GO:0005741"(mitochondrial outer membrane)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0006468"(protein phosphorylation)|go:"GO:0006915"(apoptotic process)|go:"GO:0007165"(signal transduction)|go:"GO:0007190"(activation of adenylate cyclase activity)|go:"GO:0007507"(heart development)|go:"GO:0008179"(adenylate cyclase binding)|go:"GO:0008285"(negative regulation of cell population proliferation)|go:"GO:0010856"(adenylate cyclase activator activity)|go:"GO:0016607"(nuclear speck)|go:"GO:0019899"(enzyme binding)|go:"GO:0030154"(cell differentiation)|go:"GO:0030878"(thyroid gland development)|go:"GO:0031143"(pseudopodium)|go:"GO:0031333"(negative regulation of protein-containing complex assembly)|go:"GO:0031434"(mitogen-activated protein kinase kinase binding)|go:"GO:0033138"(positive regulation of peptidyl-serine phosphorylation)|go:"GO:0035019"(somatic stem cell population maintenance)|go:"GO:0035023"(regulation of Rho protein signal transduction)|go:"GO:0035773"(insulin secretion involved in cellular response to glucose stimulus)|go:"GO:0035994"(response to muscle stretch)|go:"GO:0042060"(wound healing)|go:"GO:0042802"(identical protein binding)|go:"GO:0042981"(regulation of apoptotic process)|go:"GO:0043066"(negative regulation of apoptotic process)|go:"GO:0043154"(negative regulation of cysteine-type endopeptidase activity involved in apoptotic process)|go:"GO:0044877"(protein-containing complex binding)|go:"GO:0045104"(intermediate filament cytoskeleton organization)|go:"GO:0045595"(regulation of cell differentiation)|go:"GO:0045944"(positive regulation of transcription by RNA polymerase II)|go:"GO:0046872"(metal ion binding)|go:"GO:0048011"(neurotrophin TRK receptor signaling pathway)|go:"GO:0048538"(thymus development)|go:"GO:0060324"(face development)|go:"GO:0071550"(death-inducing signaling complex assembly)|go:"GO:0106310"(protein serine kinase activity)|go:"GO:0106311"|go:"GO:1902042"(negative regulation of extrinsic apoptotic signaling pathway via death domain receptors)|go:"GO:2000145"(regulation of cell motility)|interpro:IPR000719(Protein kinase, core)|interpro:IPR001245(Tyrosine protein kinase)|interpro:IPR002219(Protein kinase C, phorbol ester/diacylglycerol binding)|interpro:IPR003116(Raf-like Ras-binding)|interpro:IPR008271(Serine/threonine protein kinase, active site)|interpro:IPR011009(Protein kinase-like)|interpro:IPR017441(Protein kinase ATP binding, conserved site)|interpro:IPR020454|interpro:IPR029071|mint:P04049|rcsb pdb:1C1Y|rcsb pdb:1FAQ|rcsb pdb:1FAR|rcsb pdb:1GUA|rcsb pdb:1RFA|rcsb pdb:3CU8|rcsb pdb:3IQJ|rcsb pdb:3IQU|rcsb pdb:7JHP|reactome:R-HSA-2672351|reactome:R-HSA-392517|reactome:R-HSA-430116|reactome:R-HSA-5621575|reactome:R-HSA-5673000|reactome:R-HSA-5674135|reactome:R-HSA-5674499|reactome:R-HSA-5675221|reactome:R-HSA-6802946|reactome:R-HSA-6802948|reactome:R-HSA-6802952|reactome:R-HSA-6802955|reactome:R-HSA-9649948|reactome:R-HSA-9656223|reactome:R-HSA-9660537|refseq:NP_002871.1|refseq:XP_011532276.1|refseq:XP_005265412.1|dip:DIP-1048N	-	-	-	figure legend:4 C, 6C|agonist:The MEK inhibitor  PD0325901|antagonist:Trametinib - a newer MEK inhibitor|dataset:Cancer - Interactions investigated in the context of cancer|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:9606(human-a_549)|taxid:9606(Homo sapiens Caucasian lung carcinoma cell line)	-	2014/06/12	2014/10/16	rogid:ieBLcDINn9hZFZZEIQ3Ktdf2NF89606	rogid:1WOAPuzcZdMoq9hyFD/qr4Tz7049606	intact-crc:21E9E6BE3E83B70A|rigid:VLl+5T+aNh8kZKnWh1WwvnrN0PI	false	-	-	-	-	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)
uniprotkb:Q02750	uniprotkb:P10398	intact:EBI-492564|ensembl:ENSP00000302486	intact:EBI-365961|uniprotkb:P07557|uniprotkb:Q5H9B3|intact:EBI-28988863|ensembl:ENSP00000366244|uniprotkb:Q5H9B2	psi-mi:mp2k1_human(display_long)|uniprotkb:ERK activator kinase 1(gene name synonym)|uniprotkb:MAPK/ERK kinase 1(gene name synonym)|uniprotkb:MAP2K1(gene name)|psi-mi:MAP2K1(display_short)|uniprotkb:MEK1(gene name synonym)|uniprotkb:PRKMK1(gene name synonym)	psi-mi:araf_human(display_long)|uniprotkb:ARAF(gene name)|psi-mi:ARAF(display_short)|uniprotkb:PKS(gene name synonym)|uniprotkb:PKS2(gene name synonym)|uniprotkb:ARAF1(gene name synonym)|uniprotkb:Proto-oncogene A-Raf-1(gene name synonym)|uniprotkb:Proto-oncogene Pks(gene name synonym)|uniprotkb:Proto-oncogene A-Raf(gene name synonym)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Lito et al. (2014)	pubmed:24746704|imex:IM-22983	taxid:9606(human)|taxid:9606(Homo sapiens)	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-9549313|imex:IM-22983-17	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_002746.1|refseq:XP_016877900.1|dip:DIP-201N|ensembl:ENSG00000169032(gene)|ensembl:ENST00000307102(transcript)|go:"GO:0000187"(obsolete activation of MAPK activity)|go:"GO:0004672"(protein kinase activity)|go:"GO:0004674"(protein serine/threonine kinase activity)|go:"GO:0004708"(MAP kinase kinase activity)|go:"GO:0004712"(protein serine/threonine/tyrosine kinase activity)|go:"GO:0004714"(transmembrane receptor protein tyrosine kinase activity)|go:"GO:0005078"(MAP-kinase scaffold activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005634"(nucleus)|go:"GO:0005739"(mitochondrion)|go:"GO:0005769"(early endosome)|go:"GO:0005770"(late endosome)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005815"(microtubule organizing center)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0005925"(focal adhesion)|go:"GO:0006468"(protein phosphorylation)|go:"GO:0006935"(chemotaxis)|go:"GO:0007165"(signal transduction)|go:"GO:0007507"(heart development)|go:"GO:0008022"(protein C-terminus binding)|go:"GO:0008285"(negative regulation of cell population proliferation)|go:"GO:0010628"(positive regulation of gene expression)|go:"GO:0018107"(peptidyl-threonine phosphorylation)|go:"GO:0021697"(cerebellar cortex formation)|go:"GO:0030182"(neuron differentiation)|go:"GO:0030216"(keratinocyte differentiation)|go:"GO:0030878"(thyroid gland development)|go:"GO:0032872"(regulation of stress-activated MAPK cascade)|go:"GO:0043539"(protein serine/threonine kinase activator activity)|go:"GO:0045893"(positive regulation of transcription, DNA-templated)|go:"GO:0047485"(protein N-terminus binding)|go:"GO:0048538"(thymus development)|go:"GO:0048679"(regulation of axon regeneration)|go:"GO:0048870"(cell motility)|go:"GO:0050772"(positive regulation of axonogenesis)|go:"GO:0051726"(regulation of cell cycle)|go:"GO:0060020"(Bergmann glial cell differentiation)|go:"GO:0060324"(face development)|go:"GO:0060440"(trachea formation)|go:"GO:0060502"(epithelial cell proliferation involved in lung morphogenesis)|go:"GO:0060674"(placenta blood vessel development)|go:"GO:0060711"(labyrinthine layer development)|go:"GO:0070371"(ERK1 and ERK2 cascade)|go:"GO:0070374"(positive regulation of ERK1 and ERK2 cascade)|go:"GO:0071902"(positive regulation of protein serine/threonine kinase activity)|go:"GO:0090170"(regulation of Golgi inheritance)|go:"GO:0090398"(cellular senescence)|go:"GO:0097110"(scaffold protein binding)|go:"GO:0106310"(protein serine kinase activity)|go:"GO:0106311"|go:"GO:1903800"(positive regulation of production of miRNAs involved in gene silencing by miRNA)|go:"GO:2000641"(regulation of early endosome to late endosome transport)|interpro:IPR000719(Protein kinase, core)|interpro:IPR008271(Serine/threonine protein kinase, active site)|interpro:IPR011009(Protein kinase-like)|interpro:IPR017441(Protein kinase ATP binding, conserved site)|mint:Q02750|rcsb pdb:1S9J|rcsb pdb:2P55|rcsb pdb:3DV3|rcsb pdb:3DY7|rcsb pdb:3E8N|rcsb pdb:3EQB|rcsb pdb:3EQC|rcsb pdb:3EQD|rcsb pdb:3EQF|rcsb pdb:3EQG|rcsb pdb:3EQH|rcsb pdb:3EQI|rcsb pdb:3MBL|rcsb pdb:3ORN|rcsb pdb:3OS3|rcsb pdb:3PP1|rcsb pdb:3SLS|rcsb pdb:3V01|rcsb pdb:3V04|rcsb pdb:3VVH|rcsb pdb:3W8Q|rcsb pdb:3WIG|rcsb pdb:3ZLS|rcsb pdb:3ZLW|rcsb pdb:3ZLX|rcsb pdb:3ZLY|rcsb pdb:3ZM4|rcsb pdb:4AN2|rcsb pdb:4AN3|rcsb pdb:4AN9|rcsb pdb:4ANB|rcsb pdb:4ARK|rcsb pdb:4LMN|rcsb pdb:4MNE|rcsb pdb:4U7Z|rcsb pdb:4U80|rcsb pdb:4U81|rcsb pdb:5EYM|rcsb pdb:5HZE|rcsb pdb:5YT3|rcsb pdb:6NYB|rcsb pdb:6PP9|rcsb pdb:6Q0J|rcsb pdb:6Q0T|rcsb pdb:6U2G|rcsb pdb:6V2W|rcsb pdb:6X2P|rcsb pdb:6X2S|rcsb pdb:6X2X|rcsb pdb:7B3M|rcsb pdb:7B7R|rcsb pdb:7B94|rcsb pdb:7B9L|rcsb pdb:7M0T|rcsb pdb:7M0U|rcsb pdb:7M0V|rcsb pdb:7M0W|rcsb pdb:7M0X|rcsb pdb:7M0Y|rcsb pdb:7M0Z|reactome:R-HSA-110056|reactome:R-HSA-170968|reactome:R-HSA-445144|reactome:R-HSA-5210891|reactome:R-HSA-5673000|reactome:R-HSA-5674135|reactome:R-HSA-5674499|reactome:R-HSA-5684264|reactome:R-HSA-6802946|reactome:R-HSA-6802948|reactome:R-HSA-6802952|reactome:R-HSA-6802955|reactome:R-HSA-9649948|reactome:R-HSA-9652169|reactome:R-HSA-9656223|rcsb pdb:5BX0	ensembl:ENSG00000078061(gene)|ensembl:ENST00000377045(transcript)|go:"GO:0004672"(protein kinase activity)|go:"GO:0004674"(protein serine/threonine kinase activity)|go:"GO:0004709"(MAP kinase kinase kinase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005739"(mitochondrion)|go:"GO:0005829"(cytosol)|go:"GO:0006464"(cellular protein modification process)|go:"GO:0032006"(regulation of TOR signaling)|go:"GO:0032434"(regulation of proteasomal ubiquitin-dependent protein catabolic process)|go:"GO:0033138"(positive regulation of peptidyl-serine phosphorylation)|go:"GO:0043066"(negative regulation of apoptotic process)|go:"GO:0046872"(metal ion binding)|go:"GO:0106310"(protein serine kinase activity)|go:"GO:0106311"|interpro:IPR000719(Protein kinase, core)|interpro:IPR001245(Tyrosine protein kinase)|interpro:IPR002219(Protein kinase C, phorbol ester/diacylglycerol binding)|interpro:IPR003116(Raf-like Ras-binding)|interpro:IPR008271(Serine/threonine protein kinase, active site)|interpro:IPR011009(Protein kinase-like)|interpro:IPR017441(Protein kinase ATP binding, conserved site)|interpro:IPR020454|interpro:IPR029071|mint:P10398|rcsb pdb:2MSE|reactome:R-HSA-5673000|reactome:R-HSA-5674135|reactome:R-HSA-5675221|reactome:R-HSA-6802946|reactome:R-HSA-6802948|reactome:R-HSA-6802952|reactome:R-HSA-6802955|reactome:R-HSA-9649948|reactome:R-HSA-9656223|reactome:R-HSA-9660537|rcsb pdb:1WXM|refseq:NP_001645.1|refseq:XP_011542210.1|refseq:NP_001243125.1|ensembl:ENST00000377039|ensembl:ENSP00000366238|ensembl:ENSP00000366244|refseq:NP_001243126.1|dip:DIP-31374N	-	-	-	figure legend:4 C, 6C|agonist:The MEK inhibitor  PD0325901|antagonist:Trametinib - a newer MEK inhibitor|dataset:Cancer - Interactions investigated in the context of cancer|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:9606(human-a_549)|taxid:9606(Homo sapiens Caucasian lung carcinoma cell line)	-	2014/06/12	2014/10/16	rogid:ieBLcDINn9hZFZZEIQ3Ktdf2NF89606	rogid:WRtbdrEuDxNkA6jRRxvapodFcUE9606	intact-crc:21E9E6BE3E83B70A|rigid:VLl+5T+aNh8kZKnWh1WwvnrN0PI	false	-	-	-	-	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)
uniprotkb:Q02750	uniprotkb:P15056	intact:EBI-492564|ensembl:ENSP00000302486	intact:EBI-365980|uniprotkb:Q13878|uniprotkb:Q3MIN6|intact:EBI-28988924|ensembl:ENSP00000493543|uniprotkb:Q9UDP8|uniprotkb:Q9Y6T3|uniprotkb:A4D1T4|uniprotkb:B6HY61|uniprotkb:B6HY62|uniprotkb:B6HY63|uniprotkb:B6HY64|uniprotkb:B6HY65|uniprotkb:B6HY66	psi-mi:mp2k1_human(display_long)|uniprotkb:ERK activator kinase 1(gene name synonym)|uniprotkb:MAPK/ERK kinase 1(gene name synonym)|uniprotkb:MAP2K1(gene name)|psi-mi:MAP2K1(display_short)|uniprotkb:MEK1(gene name synonym)|uniprotkb:PRKMK1(gene name synonym)	psi-mi:braf_human(display_long)|uniprotkb:BRAF(gene name)|psi-mi:BRAF(display_short)|uniprotkb:BRAF1(gene name synonym)|uniprotkb:RAFB1(gene name synonym)|uniprotkb:v-Raf murine sarcoma viral oncogene homolog B1(gene name synonym)|uniprotkb:p94(gene name synonym)|uniprotkb:Proto-oncogene B-Raf(gene name synonym)	psi-mi:"MI:0107"(surface plasmon resonance)	Lito et al. (2014)	pubmed:24746704|imex:IM-22983	taxid:9606(human)|taxid:9606(Homo sapiens)	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0469"(IntAct)	intact:EBI-9549477|imex:IM-22983-4	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_002746.1|refseq:XP_016877900.1|dip:DIP-201N|ensembl:ENSG00000169032(gene)|ensembl:ENST00000307102(transcript)|go:"GO:0000187"(obsolete activation of MAPK activity)|go:"GO:0004672"(protein kinase activity)|go:"GO:0004674"(protein serine/threonine kinase activity)|go:"GO:0004708"(MAP kinase kinase activity)|go:"GO:0004712"(protein serine/threonine/tyrosine kinase activity)|go:"GO:0004714"(transmembrane receptor protein tyrosine kinase activity)|go:"GO:0005078"(MAP-kinase scaffold activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005634"(nucleus)|go:"GO:0005739"(mitochondrion)|go:"GO:0005769"(early endosome)|go:"GO:0005770"(late endosome)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005815"(microtubule organizing center)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0005925"(focal adhesion)|go:"GO:0006468"(protein phosphorylation)|go:"GO:0006935"(chemotaxis)|go:"GO:0007165"(signal transduction)|go:"GO:0007507"(heart development)|go:"GO:0008022"(protein C-terminus binding)|go:"GO:0008285"(negative regulation of cell population proliferation)|go:"GO:0010628"(positive regulation of gene expression)|go:"GO:0018107"(peptidyl-threonine phosphorylation)|go:"GO:0021697"(cerebellar cortex formation)|go:"GO:0030182"(neuron differentiation)|go:"GO:0030216"(keratinocyte differentiation)|go:"GO:0030878"(thyroid gland development)|go:"GO:0032872"(regulation of stress-activated MAPK cascade)|go:"GO:0043539"(protein serine/threonine kinase activator activity)|go:"GO:0045893"(positive regulation of transcription, DNA-templated)|go:"GO:0047485"(protein N-terminus binding)|go:"GO:0048538"(thymus development)|go:"GO:0048679"(regulation of axon regeneration)|go:"GO:0048870"(cell motility)|go:"GO:0050772"(positive regulation of axonogenesis)|go:"GO:0051726"(regulation of cell cycle)|go:"GO:0060020"(Bergmann glial cell differentiation)|go:"GO:0060324"(face development)|go:"GO:0060440"(trachea formation)|go:"GO:0060502"(epithelial cell proliferation involved in lung morphogenesis)|go:"GO:0060674"(placenta blood vessel development)|go:"GO:0060711"(labyrinthine layer development)|go:"GO:0070371"(ERK1 and ERK2 cascade)|go:"GO:0070374"(positive regulation of ERK1 and ERK2 cascade)|go:"GO:0071902"(positive regulation of protein serine/threonine kinase activity)|go:"GO:0090170"(regulation of Golgi inheritance)|go:"GO:0090398"(cellular senescence)|go:"GO:0097110"(scaffold protein binding)|go:"GO:0106310"(protein serine kinase activity)|go:"GO:0106311"|go:"GO:1903800"(positive regulation of production of miRNAs involved in gene silencing by miRNA)|go:"GO:2000641"(regulation of early endosome to late endosome transport)|interpro:IPR000719(Protein kinase, core)|interpro:IPR008271(Serine/threonine protein kinase, active site)|interpro:IPR011009(Protein kinase-like)|interpro:IPR017441(Protein kinase ATP binding, conserved site)|mint:Q02750|rcsb pdb:1S9J|rcsb pdb:2P55|rcsb pdb:3DV3|rcsb pdb:3DY7|rcsb pdb:3E8N|rcsb pdb:3EQB|rcsb pdb:3EQC|rcsb pdb:3EQD|rcsb pdb:3EQF|rcsb pdb:3EQG|rcsb pdb:3EQH|rcsb pdb:3EQI|rcsb pdb:3MBL|rcsb pdb:3ORN|rcsb pdb:3OS3|rcsb pdb:3PP1|rcsb pdb:3SLS|rcsb pdb:3V01|rcsb pdb:3V04|rcsb pdb:3VVH|rcsb pdb:3W8Q|rcsb pdb:3WIG|rcsb pdb:3ZLS|rcsb pdb:3ZLW|rcsb pdb:3ZLX|rcsb pdb:3ZLY|rcsb pdb:3ZM4|rcsb pdb:4AN2|rcsb pdb:4AN3|rcsb pdb:4AN9|rcsb pdb:4ANB|rcsb pdb:4ARK|rcsb pdb:4LMN|rcsb pdb:4MNE|rcsb pdb:4U7Z|rcsb pdb:4U80|rcsb pdb:4U81|rcsb pdb:5EYM|rcsb pdb:5HZE|rcsb pdb:5YT3|rcsb pdb:6NYB|rcsb pdb:6PP9|rcsb pdb:6Q0J|rcsb pdb:6Q0T|rcsb pdb:6U2G|rcsb pdb:6V2W|rcsb pdb:6X2P|rcsb pdb:6X2S|rcsb pdb:6X2X|rcsb pdb:7B3M|rcsb pdb:7B7R|rcsb pdb:7B94|rcsb pdb:7B9L|rcsb pdb:7M0T|rcsb pdb:7M0U|rcsb pdb:7M0V|rcsb pdb:7M0W|rcsb pdb:7M0X|rcsb pdb:7M0Y|rcsb pdb:7M0Z|reactome:R-HSA-110056|reactome:R-HSA-170968|reactome:R-HSA-445144|reactome:R-HSA-5210891|reactome:R-HSA-5673000|reactome:R-HSA-5674135|reactome:R-HSA-5674499|reactome:R-HSA-5684264|reactome:R-HSA-6802946|reactome:R-HSA-6802948|reactome:R-HSA-6802952|reactome:R-HSA-6802955|reactome:R-HSA-9649948|reactome:R-HSA-9652169|reactome:R-HSA-9656223|rcsb pdb:5BX0	ensembl:ENSG00000157764(gene)|ensembl:ENST00000646891(transcript)|go:"GO:0000165"(MAPK cascade)|go:"GO:0004672"(protein kinase activity)|go:"GO:0004674"(protein serine/threonine kinase activity)|go:"GO:0004708"(MAP kinase kinase activity)|go:"GO:0005509"(calcium ion binding)|go:"GO:0005524"(ATP binding)|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0006468"(protein phosphorylation)|go:"GO:0007173"(epidermal growth factor receptor signaling pathway)|go:"GO:0009887"(animal organ morphogenesis)|go:"GO:0010628"(positive regulation of gene expression)|go:"GO:0010828"(positive regulation of glucose transmembrane transport)|go:"GO:0033138"(positive regulation of peptidyl-serine phosphorylation)|rcsb pdb:7M0T|rcsb pdb:7M0U|rcsb pdb:7M0V|go:"GO:0042802"(identical protein binding)|rcsb pdb:7M0W|rcsb pdb:7M0X|rcsb pdb:7M0Y|rcsb pdb:7M0Z|reactome:R-HSA-1295596|reactome:R-HSA-170968|reactome:R-HSA-170984|reactome:R-HSA-187706|reactome:R-HSA-5673000|reactome:R-HSA-5674135|reactome:R-HSA-5674499|reactome:R-HSA-5675221|reactome:R-HSA-6802946|reactome:R-HSA-6802948|reactome:R-HSA-6802952|reactome:R-HSA-6802955|reactome:R-HSA-9649948|reactome:R-HSA-9656223|reactome:R-HSA-9660537|rcsb pdb:4FK3|rcsb pdb:4G9C|rcsb pdb:4G9R|rcsb pdb:4H58|rcsb pdb:4JVG|rcsb pdb:4KSP|rcsb pdb:4KSQ|rcsb pdb:4FC0|rcsb pdb:4MBJ|rcsb pdb:4MNE|rcsb pdb:4MNF|rcsb pdb:4PP7|rcsb pdb:4R5Y|rcsb pdb:4RZV|rcsb pdb:4RZW|rcsb pdb:4WO5|rcsb pdb:4XV1|rcsb pdb:4XV2|rcsb pdb:4XV3|rcsb pdb:4XV9|rcsb pdb:4YHT|rcsb pdb:5C9C|rcsb pdb:5CSW|rcsb pdb:5CSX|rcsb pdb:5CT7|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0070374"(positive regulation of ERK1 and ERK2 cascade)|go:"GO:0043066"(negative regulation of apoptotic process)|go:"GO:0070413"(trehalose metabolism in response to stress)|go:"GO:0071277"(cellular response to calcium ion)|go:"GO:0090150"(establishment of protein localization to membrane)|go:"GO:0097110"(scaffold protein binding)|go:"GO:0106310"(protein serine kinase activity)|go:"GO:0106311"|interpro:IPR000719(Protein kinase, core)|interpro:IPR001245(Tyrosine protein kinase)|interpro:IPR002219(Protein kinase C, phorbol ester/diacylglycerol binding)|interpro:IPR003116(Raf-like Ras-binding)|interpro:IPR008271(Serine/threonine protein kinase, active site)|interpro:IPR011009(Protein kinase-like)|interpro:IPR017441(Protein kinase ATP binding, conserved site)|interpro:IPR020454|rcsb pdb:5FD2|rcsb pdb:5HI2|rcsb pdb:5HID|rcsb pdb:5HIE|rcsb pdb:5ITA|rcsb pdb:5J17|rcsb pdb:5J18|rcsb pdb:5J2R|rcsb pdb:5JRQ|rcsb pdb:5JSM|rcsb pdb:5JT2|rcsb pdb:5VAL|rcsb pdb:5VAM|rcsb pdb:5VR3|rcsb pdb:5VYK|rcsb pdb:6B8U|rcsb pdb:6CAD|rcsb pdb:6N0P|rcsb pdb:6N0Q|rcsb pdb:6NSQ|rcsb pdb:6NYB|rcsb pdb:6P3D|rcsb pdb:6P7G|rcsb pdb:6PP9|rcsb pdb:6Q0J|rcsb pdb:6Q0K|rcsb pdb:6Q0T|rcsb pdb:6U2G|rcsb pdb:6U2H|rcsb pdb:6UAN|rcsb pdb:6UUO|rcsb pdb:6V2U|rcsb pdb:6V2W|rcsb pdb:6XAG|mint:P15056|rcsb pdb:1UWH|rcsb pdb:1UWJ|rcsb pdb:2FB8|rcsb pdb:2L05|rcsb pdb:3C4C|interpro:IPR029071|rcsb pdb:3D4Q|rcsb pdb:3IDP|rcsb pdb:3II5|rcsb pdb:3NY5|rcsb pdb:3OG7|rcsb pdb:3PPJ|rcsb pdb:3PPK|rcsb pdb:3PRF|rcsb pdb:3PRI|rcsb pdb:3PSB|rcsb pdb:3PSD|rcsb pdb:3Q4C|rcsb pdb:3Q96|rcsb pdb:3SKC|rcsb pdb:3TV4|rcsb pdb:3TV6|rcsb pdb:4CQE|rcsb pdb:4DBN|rcsb pdb:4E26|rcsb pdb:4E4X|rcsb pdb:4EHE|rcsb pdb:4EHG|refseq:NP_004324.2|ensembl:ENSP00000493543|dip:DIP-1045N	-	-	-	figure legend:6 E/F|comment:Trametinib is a MEK inhibitor that reduces the affinity of MEK and RAF.|dataset:Cancer - Interactions investigated in the context of cancer|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2014/06/12	2014/10/16	rogid:ieBLcDINn9hZFZZEIQ3Ktdf2NF89606	rogid:sfzILpNpX8UFB/vgH9LOKLpl/+g9606	intact-crc:FA1BEC1BF435E49E|rigid:Xwk0BUGvJhVn2aM8KIPYAmQJ6uU	false	his tag:n-n	glutathione s tranferase tag:n-n|sufficient binding region:433-726(IPR011009)	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
uniprotkb:Q02750	uniprotkb:P04049-1	intact:EBI-492564|ensembl:ENSP00000302486	intact:EBI-9549505|ensembl:ENSP00000251849	psi-mi:mp2k1_human(display_long)|uniprotkb:ERK activator kinase 1(gene name synonym)|uniprotkb:MAPK/ERK kinase 1(gene name synonym)|uniprotkb:MAP2K1(gene name)|psi-mi:MAP2K1(display_short)|uniprotkb:MEK1(gene name synonym)|uniprotkb:PRKMK1(gene name synonym)	psi-mi:p04049-1(display_long)|uniprotkb:6C(isoform synonym)|uniprotkb:RAF1(gene name)|psi-mi:RAF1(display_short)|uniprotkb:RAF(gene name synonym)|uniprotkb:Proto-oncogene c-RAF(gene name synonym)|uniprotkb:Raf-1(gene name synonym)	psi-mi:"MI:0107"(surface plasmon resonance)	Lito et al. (2014)	pubmed:24746704|imex:IM-22983	taxid:9606(human)|taxid:9606(Homo sapiens)	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0469"(IntAct)	intact:EBI-9549493|imex:IM-22983-5	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_002746.1|refseq:XP_016877900.1|dip:DIP-201N|ensembl:ENSG00000169032(gene)|ensembl:ENST00000307102(transcript)|go:"GO:0000187"(obsolete activation of MAPK activity)|go:"GO:0004672"(protein kinase activity)|go:"GO:0004674"(protein serine/threonine kinase activity)|go:"GO:0004708"(MAP kinase kinase activity)|go:"GO:0004712"(protein serine/threonine/tyrosine kinase activity)|go:"GO:0004714"(transmembrane receptor protein tyrosine kinase activity)|go:"GO:0005078"(MAP-kinase scaffold activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005634"(nucleus)|go:"GO:0005739"(mitochondrion)|go:"GO:0005769"(early endosome)|go:"GO:0005770"(late endosome)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005815"(microtubule organizing center)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0005925"(focal adhesion)|go:"GO:0006468"(protein phosphorylation)|go:"GO:0006935"(chemotaxis)|go:"GO:0007165"(signal transduction)|go:"GO:0007507"(heart development)|go:"GO:0008022"(protein C-terminus binding)|go:"GO:0008285"(negative regulation of cell population proliferation)|go:"GO:0010628"(positive regulation of gene expression)|go:"GO:0018107"(peptidyl-threonine phosphorylation)|go:"GO:0021697"(cerebellar cortex formation)|go:"GO:0030182"(neuron differentiation)|go:"GO:0030216"(keratinocyte differentiation)|go:"GO:0030878"(thyroid gland development)|go:"GO:0032872"(regulation of stress-activated MAPK cascade)|go:"GO:0043539"(protein serine/threonine kinase activator activity)|go:"GO:0045893"(positive regulation of transcription, DNA-templated)|go:"GO:0047485"(protein N-terminus binding)|go:"GO:0048538"(thymus development)|go:"GO:0048679"(regulation of axon regeneration)|go:"GO:0048870"(cell motility)|go:"GO:0050772"(positive regulation of axonogenesis)|go:"GO:0051726"(regulation of cell cycle)|go:"GO:0060020"(Bergmann glial cell differentiation)|go:"GO:0060324"(face development)|go:"GO:0060440"(trachea formation)|go:"GO:0060502"(epithelial cell proliferation involved in lung morphogenesis)|go:"GO:0060674"(placenta blood vessel development)|go:"GO:0060711"(labyrinthine layer development)|go:"GO:0070371"(ERK1 and ERK2 cascade)|go:"GO:0070374"(positive regulation of ERK1 and ERK2 cascade)|go:"GO:0071902"(positive regulation of protein serine/threonine kinase activity)|go:"GO:0090170"(regulation of Golgi inheritance)|go:"GO:0090398"(cellular senescence)|go:"GO:0097110"(scaffold protein binding)|go:"GO:0106310"(protein serine kinase activity)|go:"GO:0106311"|go:"GO:1903800"(positive regulation of production of miRNAs involved in gene silencing by miRNA)|go:"GO:2000641"(regulation of early endosome to late endosome transport)|interpro:IPR000719(Protein kinase, core)|interpro:IPR008271(Serine/threonine protein kinase, active site)|interpro:IPR011009(Protein kinase-like)|interpro:IPR017441(Protein kinase ATP binding, conserved site)|mint:Q02750|rcsb pdb:1S9J|rcsb pdb:2P55|rcsb pdb:3DV3|rcsb pdb:3DY7|rcsb pdb:3E8N|rcsb pdb:3EQB|rcsb pdb:3EQC|rcsb pdb:3EQD|rcsb pdb:3EQF|rcsb pdb:3EQG|rcsb pdb:3EQH|rcsb pdb:3EQI|rcsb pdb:3MBL|rcsb pdb:3ORN|rcsb pdb:3OS3|rcsb pdb:3PP1|rcsb pdb:3SLS|rcsb pdb:3V01|rcsb pdb:3V04|rcsb pdb:3VVH|rcsb pdb:3W8Q|rcsb pdb:3WIG|rcsb pdb:3ZLS|rcsb pdb:3ZLW|rcsb pdb:3ZLX|rcsb pdb:3ZLY|rcsb pdb:3ZM4|rcsb pdb:4AN2|rcsb pdb:4AN3|rcsb pdb:4AN9|rcsb pdb:4ANB|rcsb pdb:4ARK|rcsb pdb:4LMN|rcsb pdb:4MNE|rcsb pdb:4U7Z|rcsb pdb:4U80|rcsb pdb:4U81|rcsb pdb:5EYM|rcsb pdb:5HZE|rcsb pdb:5YT3|rcsb pdb:6NYB|rcsb pdb:6PP9|rcsb pdb:6Q0J|rcsb pdb:6Q0T|rcsb pdb:6U2G|rcsb pdb:6V2W|rcsb pdb:6X2P|rcsb pdb:6X2S|rcsb pdb:6X2X|rcsb pdb:7B3M|rcsb pdb:7B7R|rcsb pdb:7B94|rcsb pdb:7B9L|rcsb pdb:7M0T|rcsb pdb:7M0U|rcsb pdb:7M0V|rcsb pdb:7M0W|rcsb pdb:7M0X|rcsb pdb:7M0Y|rcsb pdb:7M0Z|reactome:R-HSA-110056|reactome:R-HSA-170968|reactome:R-HSA-445144|reactome:R-HSA-5210891|reactome:R-HSA-5673000|reactome:R-HSA-5674135|reactome:R-HSA-5674499|reactome:R-HSA-5684264|reactome:R-HSA-6802946|reactome:R-HSA-6802948|reactome:R-HSA-6802952|reactome:R-HSA-6802955|reactome:R-HSA-9649948|reactome:R-HSA-9652169|reactome:R-HSA-9656223|rcsb pdb:5BX0	ensembl:ENSG00000132155(gene)|ensembl:ENST00000251849(transcript)|intact:EBI-365996(isoform-parent)	-	-	-	figure legend:6 E/F|comment:Trametinib is a MEK inhibitor that reduces the affinity of MEK and RAF.|dataset:Cancer - Interactions investigated in the context of cancer|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2014/06/12	2015/05/26	rogid:ieBLcDINn9hZFZZEIQ3Ktdf2NF89606	rogid:1WOAPuzcZdMoq9hyFD/qr4Tz7049606	intact-crc:C528E83FC7547CC1|rigid:PBGsilY2R+EvyAAA1Mf4R6U2sdY	false	his tag:n-n	glutathione s tranferase tag:n-n|sufficient binding region:306-648(IPR011009)|mutation:340-341	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
chebi:"CHEBI:78825"	uniprotkb:Q02750	intact:EBI-9549536	intact:EBI-492564|ensembl:ENSP00000302486	psi-mi:ch5126766(display_short)|psi-mi:"CHEBI:78825"(display_long)	psi-mi:mp2k1_human(display_long)|uniprotkb:ERK activator kinase 1(gene name synonym)|uniprotkb:MAPK/ERK kinase 1(gene name synonym)|uniprotkb:MAP2K1(gene name)|psi-mi:MAP2K1(display_short)|uniprotkb:MEK1(gene name synonym)|uniprotkb:PRKMK1(gene name synonym)	psi-mi:"MI:0114"(x-ray crystallography)	Lito et al. (2014)	pubmed:24746704|imex:IM-22983	taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)")	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0469"(IntAct)	intact:EBI-9549528|imex:IM-22983-8|wwpdb:3wig	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:1160"(allosteric effector)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0328"(small molecule)	psi-mi:"MI:0326"(protein)	-	refseq:NP_002746.1|refseq:XP_016877900.1|dip:DIP-201N|ensembl:ENSG00000169032(gene)|ensembl:ENST00000307102(transcript)|go:"GO:0000187"(obsolete activation of MAPK activity)|go:"GO:0004672"(protein kinase activity)|go:"GO:0004674"(protein serine/threonine kinase activity)|go:"GO:0004708"(MAP kinase kinase activity)|go:"GO:0004712"(protein serine/threonine/tyrosine kinase activity)|go:"GO:0004714"(transmembrane receptor protein tyrosine kinase activity)|go:"GO:0005078"(MAP-kinase scaffold activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005634"(nucleus)|go:"GO:0005739"(mitochondrion)|go:"GO:0005769"(early endosome)|go:"GO:0005770"(late endosome)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005815"(microtubule organizing center)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0005925"(focal adhesion)|go:"GO:0006468"(protein phosphorylation)|go:"GO:0006935"(chemotaxis)|go:"GO:0007165"(signal transduction)|go:"GO:0007507"(heart development)|go:"GO:0008022"(protein C-terminus binding)|go:"GO:0008285"(negative regulation of cell population proliferation)|go:"GO:0010628"(positive regulation of gene expression)|go:"GO:0018107"(peptidyl-threonine phosphorylation)|go:"GO:0021697"(cerebellar cortex formation)|go:"GO:0030182"(neuron differentiation)|go:"GO:0030216"(keratinocyte differentiation)|go:"GO:0030878"(thyroid gland development)|go:"GO:0032872"(regulation of stress-activated MAPK cascade)|go:"GO:0043539"(protein serine/threonine kinase activator activity)|go:"GO:0045893"(positive regulation of transcription, DNA-templated)|go:"GO:0047485"(protein N-terminus binding)|go:"GO:0048538"(thymus development)|go:"GO:0048679"(regulation of axon regeneration)|go:"GO:0048870"(cell motility)|go:"GO:0050772"(positive regulation of axonogenesis)|go:"GO:0051726"(regulation of cell cycle)|go:"GO:0060020"(Bergmann glial cell differentiation)|go:"GO:0060324"(face development)|go:"GO:0060440"(trachea formation)|go:"GO:0060502"(epithelial cell proliferation involved in lung morphogenesis)|go:"GO:0060674"(placenta blood vessel development)|go:"GO:0060711"(labyrinthine layer development)|go:"GO:0070371"(ERK1 and ERK2 cascade)|go:"GO:0070374"(positive regulation of ERK1 and ERK2 cascade)|go:"GO:0071902"(positive regulation of protein serine/threonine kinase activity)|go:"GO:0090170"(regulation of Golgi inheritance)|go:"GO:0090398"(cellular senescence)|go:"GO:0097110"(scaffold protein binding)|go:"GO:0106310"(protein serine kinase activity)|go:"GO:0106311"|go:"GO:1903800"(positive regulation of production of miRNAs involved in gene silencing by miRNA)|go:"GO:2000641"(regulation of early endosome to late endosome transport)|interpro:IPR000719(Protein kinase, core)|interpro:IPR008271(Serine/threonine protein kinase, active site)|interpro:IPR011009(Protein kinase-like)|interpro:IPR017441(Protein kinase ATP binding, conserved site)|mint:Q02750|rcsb pdb:1S9J|rcsb pdb:2P55|rcsb pdb:3DV3|rcsb pdb:3DY7|rcsb pdb:3E8N|rcsb pdb:3EQB|rcsb pdb:3EQC|rcsb pdb:3EQD|rcsb pdb:3EQF|rcsb pdb:3EQG|rcsb pdb:3EQH|rcsb pdb:3EQI|rcsb pdb:3MBL|rcsb pdb:3ORN|rcsb pdb:3OS3|rcsb pdb:3PP1|rcsb pdb:3SLS|rcsb pdb:3V01|rcsb pdb:3V04|rcsb pdb:3VVH|rcsb pdb:3W8Q|rcsb pdb:3WIG|rcsb pdb:3ZLS|rcsb pdb:3ZLW|rcsb pdb:3ZLX|rcsb pdb:3ZLY|rcsb pdb:3ZM4|rcsb pdb:4AN2|rcsb pdb:4AN3|rcsb pdb:4AN9|rcsb pdb:4ANB|rcsb pdb:4ARK|rcsb pdb:4LMN|rcsb pdb:4MNE|rcsb pdb:4U7Z|rcsb pdb:4U80|rcsb pdb:4U81|rcsb pdb:5EYM|rcsb pdb:5HZE|rcsb pdb:5YT3|rcsb pdb:6NYB|rcsb pdb:6PP9|rcsb pdb:6Q0J|rcsb pdb:6Q0T|rcsb pdb:6U2G|rcsb pdb:6V2W|rcsb pdb:6X2P|rcsb pdb:6X2S|rcsb pdb:6X2X|rcsb pdb:7B3M|rcsb pdb:7B7R|rcsb pdb:7B94|rcsb pdb:7B9L|rcsb pdb:7M0T|rcsb pdb:7M0U|rcsb pdb:7M0V|rcsb pdb:7M0W|rcsb pdb:7M0X|rcsb pdb:7M0Y|rcsb pdb:7M0Z|reactome:R-HSA-110056|reactome:R-HSA-170968|reactome:R-HSA-445144|reactome:R-HSA-5210891|reactome:R-HSA-5673000|reactome:R-HSA-5674135|reactome:R-HSA-5674499|reactome:R-HSA-5684264|reactome:R-HSA-6802946|reactome:R-HSA-6802948|reactome:R-HSA-6802952|reactome:R-HSA-6802955|reactome:R-HSA-9649948|reactome:R-HSA-9652169|reactome:R-HSA-9656223|rcsb pdb:5BX0	-	-	-	figure legend:Table S1|3d-resolution:2.7 A|3d-r-factors:"R-factor: working - 21.7%, Free R-factor: 25.0%,"|dataset:Cancer - Interactions investigated in the context of cancer|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2014/06/12	2014/10/16	-	rogid:ieBLcDINn9hZFZZEIQ3Ktdf2NF89606	intact-crc:A6E88F87BC36EE99	false	-	his tag:?-?|sufficient binding region:62-270,303-393	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
chebi:"CHEBI:78825"	chebi:"CHEBI:40749"	intact:EBI-9549536	intact:EBI-9549541	psi-mi:ch5126766(display_short)|psi-mi:"CHEBI:78825"(display_long)	psi-mi:phosphoaminophosphonic acid-adenylate ester(display_short)|psi-mi:"CHEBI:40749"(display_long)	psi-mi:"MI:0114"(x-ray crystallography)	Lito et al. (2014)	pubmed:24746704|imex:IM-22983	taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)")	taxid:-2(chemical synthesis)|taxid:-2("Chemical synthesis (Chemical synthesis)")	psi-mi:"MI:0407"(direct interaction)	psi-mi:"MI:0469"(IntAct)	intact:EBI-9549528|imex:IM-22983-8|wwpdb:3wig	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:1160"(allosteric effector)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0497"(neutral component)	psi-mi:"MI:0328"(small molecule)	psi-mi:"MI:0328"(small molecule)	-	-	-	-	-	figure legend:Table S1|3d-resolution:2.7 A|3d-r-factors:"R-factor: working - 21.7%, Free R-factor: 25.0%,"|dataset:Cancer - Interactions investigated in the context of cancer|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:-1(in vitro)|taxid:-1(In vitro)	-	2014/06/12	2014/10/16	-	-	intact-crc:A6E88F87BC36EE99	false	-	-	-	-	psi-mi:"MI:0396"(predetermined participant)	psi-mi:"MI:0396"(predetermined participant)
uniprotkb:Q02750	uniprotkb:P04049	intact:EBI-492564|ensembl:ENSP00000302486	intact:EBI-365996|uniprotkb:B0LPH8|uniprotkb:Q15278|uniprotkb:Q9UC20|ensembl:ENSP00000251849|uniprotkb:B2R5N3	psi-mi:mp2k1_human(display_long)|uniprotkb:ERK activator kinase 1(gene name synonym)|uniprotkb:MAPK/ERK kinase 1(gene name synonym)|uniprotkb:MAP2K1(gene name)|psi-mi:MAP2K1(display_short)|uniprotkb:MEK1(gene name synonym)|uniprotkb:PRKMK1(gene name synonym)	psi-mi:raf1_human(display_long)|uniprotkb:RAF1(gene name)|psi-mi:RAF1(display_short)|uniprotkb:RAF(gene name synonym)|uniprotkb:Proto-oncogene c-RAF(gene name synonym)|uniprotkb:Raf-1(gene name synonym)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Lito et al. (2014)	pubmed:24746704|imex:IM-22983	taxid:9606(human)|taxid:9606(Homo sapiens)	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-10823652|imex:IM-22983-9	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_002746.1|refseq:XP_016877900.1|dip:DIP-201N|ensembl:ENSG00000169032(gene)|ensembl:ENST00000307102(transcript)|go:"GO:0000187"(obsolete activation of MAPK activity)|go:"GO:0004672"(protein kinase activity)|go:"GO:0004674"(protein serine/threonine kinase activity)|go:"GO:0004708"(MAP kinase kinase activity)|go:"GO:0004712"(protein serine/threonine/tyrosine kinase activity)|go:"GO:0004714"(transmembrane receptor protein tyrosine kinase activity)|go:"GO:0005078"(MAP-kinase scaffold activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005634"(nucleus)|go:"GO:0005739"(mitochondrion)|go:"GO:0005769"(early endosome)|go:"GO:0005770"(late endosome)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005815"(microtubule organizing center)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0005925"(focal adhesion)|go:"GO:0006468"(protein phosphorylation)|go:"GO:0006935"(chemotaxis)|go:"GO:0007165"(signal transduction)|go:"GO:0007507"(heart development)|go:"GO:0008022"(protein C-terminus binding)|go:"GO:0008285"(negative regulation of cell population proliferation)|go:"GO:0010628"(positive regulation of gene expression)|go:"GO:0018107"(peptidyl-threonine phosphorylation)|go:"GO:0021697"(cerebellar cortex formation)|go:"GO:0030182"(neuron differentiation)|go:"GO:0030216"(keratinocyte differentiation)|go:"GO:0030878"(thyroid gland development)|go:"GO:0032872"(regulation of stress-activated MAPK cascade)|go:"GO:0043539"(protein serine/threonine kinase activator activity)|go:"GO:0045893"(positive regulation of transcription, DNA-templated)|go:"GO:0047485"(protein N-terminus binding)|go:"GO:0048538"(thymus development)|go:"GO:0048679"(regulation of axon regeneration)|go:"GO:0048870"(cell motility)|go:"GO:0050772"(positive regulation of axonogenesis)|go:"GO:0051726"(regulation of cell cycle)|go:"GO:0060020"(Bergmann glial cell differentiation)|go:"GO:0060324"(face development)|go:"GO:0060440"(trachea formation)|go:"GO:0060502"(epithelial cell proliferation involved in lung morphogenesis)|go:"GO:0060674"(placenta blood vessel development)|go:"GO:0060711"(labyrinthine layer development)|go:"GO:0070371"(ERK1 and ERK2 cascade)|go:"GO:0070374"(positive regulation of ERK1 and ERK2 cascade)|go:"GO:0071902"(positive regulation of protein serine/threonine kinase activity)|go:"GO:0090170"(regulation of Golgi inheritance)|go:"GO:0090398"(cellular senescence)|go:"GO:0097110"(scaffold protein binding)|go:"GO:0106310"(protein serine kinase activity)|go:"GO:0106311"|go:"GO:1903800"(positive regulation of production of miRNAs involved in gene silencing by miRNA)|go:"GO:2000641"(regulation of early endosome to late endosome transport)|interpro:IPR000719(Protein kinase, core)|interpro:IPR008271(Serine/threonine protein kinase, active site)|interpro:IPR011009(Protein kinase-like)|interpro:IPR017441(Protein kinase ATP binding, conserved site)|mint:Q02750|rcsb pdb:1S9J|rcsb pdb:2P55|rcsb pdb:3DV3|rcsb pdb:3DY7|rcsb pdb:3E8N|rcsb pdb:3EQB|rcsb pdb:3EQC|rcsb pdb:3EQD|rcsb pdb:3EQF|rcsb pdb:3EQG|rcsb pdb:3EQH|rcsb pdb:3EQI|rcsb pdb:3MBL|rcsb pdb:3ORN|rcsb pdb:3OS3|rcsb pdb:3PP1|rcsb pdb:3SLS|rcsb pdb:3V01|rcsb pdb:3V04|rcsb pdb:3VVH|rcsb pdb:3W8Q|rcsb pdb:3WIG|rcsb pdb:3ZLS|rcsb pdb:3ZLW|rcsb pdb:3ZLX|rcsb pdb:3ZLY|rcsb pdb:3ZM4|rcsb pdb:4AN2|rcsb pdb:4AN3|rcsb pdb:4AN9|rcsb pdb:4ANB|rcsb pdb:4ARK|rcsb pdb:4LMN|rcsb pdb:4MNE|rcsb pdb:4U7Z|rcsb pdb:4U80|rcsb pdb:4U81|rcsb pdb:5EYM|rcsb pdb:5HZE|rcsb pdb:5YT3|rcsb pdb:6NYB|rcsb pdb:6PP9|rcsb pdb:6Q0J|rcsb pdb:6Q0T|rcsb pdb:6U2G|rcsb pdb:6V2W|rcsb pdb:6X2P|rcsb pdb:6X2S|rcsb pdb:6X2X|rcsb pdb:7B3M|rcsb pdb:7B7R|rcsb pdb:7B94|rcsb pdb:7B9L|rcsb pdb:7M0T|rcsb pdb:7M0U|rcsb pdb:7M0V|rcsb pdb:7M0W|rcsb pdb:7M0X|rcsb pdb:7M0Y|rcsb pdb:7M0Z|reactome:R-HSA-110056|reactome:R-HSA-170968|reactome:R-HSA-445144|reactome:R-HSA-5210891|reactome:R-HSA-5673000|reactome:R-HSA-5674135|reactome:R-HSA-5674499|reactome:R-HSA-5684264|reactome:R-HSA-6802946|reactome:R-HSA-6802948|reactome:R-HSA-6802952|reactome:R-HSA-6802955|reactome:R-HSA-9649948|reactome:R-HSA-9652169|reactome:R-HSA-9656223|rcsb pdb:5BX0	rcsb pdb:3IQV|rcsb pdb:3KUC|rcsb pdb:3KUD|rcsb pdb:3NKX|rcsb pdb:3O8I|rcsb pdb:3OMV|rcsb pdb:4FJ3|rcsb pdb:4G0N|rcsb pdb:4G3X|rcsb pdb:4IEA|rcsb pdb:4IHL|rcsb pdb:6NTC|rcsb pdb:6NTD|rcsb pdb:6PTS|rcsb pdb:6PTW|rcsb pdb:6VJJ|rcsb pdb:6XGU|rcsb pdb:6XGV|rcsb pdb:6XHA|rcsb pdb:6XHB|rcsb pdb:6XI7|ensembl:ENSG00000132155(gene)|ensembl:ENST00000251849(transcript)|go:"GO:0000186"(obsolete activation of MAPKK activity)|go:"GO:0001666"(response to hypoxia)|go:"GO:0004672"(protein kinase activity)|go:"GO:0004674"(protein serine/threonine kinase activity)|go:"GO:0004709"(MAP kinase kinase kinase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005737"(cytoplasm)|go:"GO:0005739"(mitochondrion)|go:"GO:0005741"(mitochondrial outer membrane)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0006468"(protein phosphorylation)|go:"GO:0006915"(apoptotic process)|go:"GO:0007165"(signal transduction)|go:"GO:0007190"(activation of adenylate cyclase activity)|go:"GO:0007507"(heart development)|go:"GO:0008179"(adenylate cyclase binding)|go:"GO:0008285"(negative regulation of cell population proliferation)|go:"GO:0010856"(adenylate cyclase activator activity)|go:"GO:0016607"(nuclear speck)|go:"GO:0019899"(enzyme binding)|go:"GO:0030154"(cell differentiation)|go:"GO:0030878"(thyroid gland development)|go:"GO:0031143"(pseudopodium)|go:"GO:0031333"(negative regulation of protein-containing complex assembly)|go:"GO:0031434"(mitogen-activated protein kinase kinase binding)|go:"GO:0033138"(positive regulation of peptidyl-serine phosphorylation)|go:"GO:0035019"(somatic stem cell population maintenance)|go:"GO:0035023"(regulation of Rho protein signal transduction)|go:"GO:0035773"(insulin secretion involved in cellular response to glucose stimulus)|go:"GO:0035994"(response to muscle stretch)|go:"GO:0042060"(wound healing)|go:"GO:0042802"(identical protein binding)|go:"GO:0042981"(regulation of apoptotic process)|go:"GO:0043066"(negative regulation of apoptotic process)|go:"GO:0043154"(negative regulation of cysteine-type endopeptidase activity involved in apoptotic process)|go:"GO:0044877"(protein-containing complex binding)|go:"GO:0045104"(intermediate filament cytoskeleton organization)|go:"GO:0045595"(regulation of cell differentiation)|go:"GO:0045944"(positive regulation of transcription by RNA polymerase II)|go:"GO:0046872"(metal ion binding)|go:"GO:0048011"(neurotrophin TRK receptor signaling pathway)|go:"GO:0048538"(thymus development)|go:"GO:0060324"(face development)|go:"GO:0071550"(death-inducing signaling complex assembly)|go:"GO:0106310"(protein serine kinase activity)|go:"GO:0106311"|go:"GO:1902042"(negative regulation of extrinsic apoptotic signaling pathway via death domain receptors)|go:"GO:2000145"(regulation of cell motility)|interpro:IPR000719(Protein kinase, core)|interpro:IPR001245(Tyrosine protein kinase)|interpro:IPR002219(Protein kinase C, phorbol ester/diacylglycerol binding)|interpro:IPR003116(Raf-like Ras-binding)|interpro:IPR008271(Serine/threonine protein kinase, active site)|interpro:IPR011009(Protein kinase-like)|interpro:IPR017441(Protein kinase ATP binding, conserved site)|interpro:IPR020454|interpro:IPR029071|mint:P04049|rcsb pdb:1C1Y|rcsb pdb:1FAQ|rcsb pdb:1FAR|rcsb pdb:1GUA|rcsb pdb:1RFA|rcsb pdb:3CU8|rcsb pdb:3IQJ|rcsb pdb:3IQU|rcsb pdb:7JHP|reactome:R-HSA-2672351|reactome:R-HSA-392517|reactome:R-HSA-430116|reactome:R-HSA-5621575|reactome:R-HSA-5673000|reactome:R-HSA-5674135|reactome:R-HSA-5674499|reactome:R-HSA-5675221|reactome:R-HSA-6802946|reactome:R-HSA-6802948|reactome:R-HSA-6802952|reactome:R-HSA-6802955|reactome:R-HSA-9649948|reactome:R-HSA-9656223|reactome:R-HSA-9660537|refseq:NP_002871.1|refseq:XP_011532276.1|refseq:XP_005265412.1|dip:DIP-1048N	-	-	-	figure legend:6D|agonist:The MEK inhibitor  PD0325901|antagonist:Trametinib - a newer MEK inhibitor|dataset:Cancer - Interactions investigated in the context of cancer|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:9606(human-a_549)|taxid:9606(Homo sapiens Caucasian lung carcinoma cell line)	-	2014/06/12	2015/05/22	rogid:ieBLcDINn9hZFZZEIQ3Ktdf2NF89606	rogid:1WOAPuzcZdMoq9hyFD/qr4Tz7049606	rigid:BpXg3tpBH0abj15rihKRcuuAOPk	false	-	-	-	-	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)
uniprotkb:Q02750	uniprotkb:P15056	intact:EBI-492564|ensembl:ENSP00000302486	intact:EBI-365980|uniprotkb:Q13878|uniprotkb:Q3MIN6|intact:EBI-28988924|ensembl:ENSP00000493543|uniprotkb:Q9UDP8|uniprotkb:Q9Y6T3|uniprotkb:A4D1T4|uniprotkb:B6HY61|uniprotkb:B6HY62|uniprotkb:B6HY63|uniprotkb:B6HY64|uniprotkb:B6HY65|uniprotkb:B6HY66	psi-mi:mp2k1_human(display_long)|uniprotkb:ERK activator kinase 1(gene name synonym)|uniprotkb:MAPK/ERK kinase 1(gene name synonym)|uniprotkb:MAP2K1(gene name)|psi-mi:MAP2K1(display_short)|uniprotkb:MEK1(gene name synonym)|uniprotkb:PRKMK1(gene name synonym)	psi-mi:braf_human(display_long)|uniprotkb:BRAF(gene name)|psi-mi:BRAF(display_short)|uniprotkb:BRAF1(gene name synonym)|uniprotkb:RAFB1(gene name synonym)|uniprotkb:v-Raf murine sarcoma viral oncogene homolog B1(gene name synonym)|uniprotkb:p94(gene name synonym)|uniprotkb:Proto-oncogene B-Raf(gene name synonym)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Lito et al. (2014)	pubmed:24746704|imex:IM-22983	taxid:9606(human)|taxid:9606(Homo sapiens)	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-10823652|imex:IM-22983-9	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_002746.1|refseq:XP_016877900.1|dip:DIP-201N|ensembl:ENSG00000169032(gene)|ensembl:ENST00000307102(transcript)|go:"GO:0000187"(obsolete activation of MAPK activity)|go:"GO:0004672"(protein kinase activity)|go:"GO:0004674"(protein serine/threonine kinase activity)|go:"GO:0004708"(MAP kinase kinase activity)|go:"GO:0004712"(protein serine/threonine/tyrosine kinase activity)|go:"GO:0004714"(transmembrane receptor protein tyrosine kinase activity)|go:"GO:0005078"(MAP-kinase scaffold activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005634"(nucleus)|go:"GO:0005739"(mitochondrion)|go:"GO:0005769"(early endosome)|go:"GO:0005770"(late endosome)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005815"(microtubule organizing center)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0005925"(focal adhesion)|go:"GO:0006468"(protein phosphorylation)|go:"GO:0006935"(chemotaxis)|go:"GO:0007165"(signal transduction)|go:"GO:0007507"(heart development)|go:"GO:0008022"(protein C-terminus binding)|go:"GO:0008285"(negative regulation of cell population proliferation)|go:"GO:0010628"(positive regulation of gene expression)|go:"GO:0018107"(peptidyl-threonine phosphorylation)|go:"GO:0021697"(cerebellar cortex formation)|go:"GO:0030182"(neuron differentiation)|go:"GO:0030216"(keratinocyte differentiation)|go:"GO:0030878"(thyroid gland development)|go:"GO:0032872"(regulation of stress-activated MAPK cascade)|go:"GO:0043539"(protein serine/threonine kinase activator activity)|go:"GO:0045893"(positive regulation of transcription, DNA-templated)|go:"GO:0047485"(protein N-terminus binding)|go:"GO:0048538"(thymus development)|go:"GO:0048679"(regulation of axon regeneration)|go:"GO:0048870"(cell motility)|go:"GO:0050772"(positive regulation of axonogenesis)|go:"GO:0051726"(regulation of cell cycle)|go:"GO:0060020"(Bergmann glial cell differentiation)|go:"GO:0060324"(face development)|go:"GO:0060440"(trachea formation)|go:"GO:0060502"(epithelial cell proliferation involved in lung morphogenesis)|go:"GO:0060674"(placenta blood vessel development)|go:"GO:0060711"(labyrinthine layer development)|go:"GO:0070371"(ERK1 and ERK2 cascade)|go:"GO:0070374"(positive regulation of ERK1 and ERK2 cascade)|go:"GO:0071902"(positive regulation of protein serine/threonine kinase activity)|go:"GO:0090170"(regulation of Golgi inheritance)|go:"GO:0090398"(cellular senescence)|go:"GO:0097110"(scaffold protein binding)|go:"GO:0106310"(protein serine kinase activity)|go:"GO:0106311"|go:"GO:1903800"(positive regulation of production of miRNAs involved in gene silencing by miRNA)|go:"GO:2000641"(regulation of early endosome to late endosome transport)|interpro:IPR000719(Protein kinase, core)|interpro:IPR008271(Serine/threonine protein kinase, active site)|interpro:IPR011009(Protein kinase-like)|interpro:IPR017441(Protein kinase ATP binding, conserved site)|mint:Q02750|rcsb pdb:1S9J|rcsb pdb:2P55|rcsb pdb:3DV3|rcsb pdb:3DY7|rcsb pdb:3E8N|rcsb pdb:3EQB|rcsb pdb:3EQC|rcsb pdb:3EQD|rcsb pdb:3EQF|rcsb pdb:3EQG|rcsb pdb:3EQH|rcsb pdb:3EQI|rcsb pdb:3MBL|rcsb pdb:3ORN|rcsb pdb:3OS3|rcsb pdb:3PP1|rcsb pdb:3SLS|rcsb pdb:3V01|rcsb pdb:3V04|rcsb pdb:3VVH|rcsb pdb:3W8Q|rcsb pdb:3WIG|rcsb pdb:3ZLS|rcsb pdb:3ZLW|rcsb pdb:3ZLX|rcsb pdb:3ZLY|rcsb pdb:3ZM4|rcsb pdb:4AN2|rcsb pdb:4AN3|rcsb pdb:4AN9|rcsb pdb:4ANB|rcsb pdb:4ARK|rcsb pdb:4LMN|rcsb pdb:4MNE|rcsb pdb:4U7Z|rcsb pdb:4U80|rcsb pdb:4U81|rcsb pdb:5EYM|rcsb pdb:5HZE|rcsb pdb:5YT3|rcsb pdb:6NYB|rcsb pdb:6PP9|rcsb pdb:6Q0J|rcsb pdb:6Q0T|rcsb pdb:6U2G|rcsb pdb:6V2W|rcsb pdb:6X2P|rcsb pdb:6X2S|rcsb pdb:6X2X|rcsb pdb:7B3M|rcsb pdb:7B7R|rcsb pdb:7B94|rcsb pdb:7B9L|rcsb pdb:7M0T|rcsb pdb:7M0U|rcsb pdb:7M0V|rcsb pdb:7M0W|rcsb pdb:7M0X|rcsb pdb:7M0Y|rcsb pdb:7M0Z|reactome:R-HSA-110056|reactome:R-HSA-170968|reactome:R-HSA-445144|reactome:R-HSA-5210891|reactome:R-HSA-5673000|reactome:R-HSA-5674135|reactome:R-HSA-5674499|reactome:R-HSA-5684264|reactome:R-HSA-6802946|reactome:R-HSA-6802948|reactome:R-HSA-6802952|reactome:R-HSA-6802955|reactome:R-HSA-9649948|reactome:R-HSA-9652169|reactome:R-HSA-9656223|rcsb pdb:5BX0	ensembl:ENSG00000157764(gene)|ensembl:ENST00000646891(transcript)|go:"GO:0000165"(MAPK cascade)|go:"GO:0004672"(protein kinase activity)|go:"GO:0004674"(protein serine/threonine kinase activity)|go:"GO:0004708"(MAP kinase kinase activity)|go:"GO:0005509"(calcium ion binding)|go:"GO:0005524"(ATP binding)|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0006468"(protein phosphorylation)|go:"GO:0007173"(epidermal growth factor receptor signaling pathway)|go:"GO:0009887"(animal organ morphogenesis)|go:"GO:0010628"(positive regulation of gene expression)|go:"GO:0010828"(positive regulation of glucose transmembrane transport)|go:"GO:0033138"(positive regulation of peptidyl-serine phosphorylation)|rcsb pdb:7M0T|rcsb pdb:7M0U|rcsb pdb:7M0V|go:"GO:0042802"(identical protein binding)|rcsb pdb:7M0W|rcsb pdb:7M0X|rcsb pdb:7M0Y|rcsb pdb:7M0Z|reactome:R-HSA-1295596|reactome:R-HSA-170968|reactome:R-HSA-170984|reactome:R-HSA-187706|reactome:R-HSA-5673000|reactome:R-HSA-5674135|reactome:R-HSA-5674499|reactome:R-HSA-5675221|reactome:R-HSA-6802946|reactome:R-HSA-6802948|reactome:R-HSA-6802952|reactome:R-HSA-6802955|reactome:R-HSA-9649948|reactome:R-HSA-9656223|reactome:R-HSA-9660537|rcsb pdb:4FK3|rcsb pdb:4G9C|rcsb pdb:4G9R|rcsb pdb:4H58|rcsb pdb:4JVG|rcsb pdb:4KSP|rcsb pdb:4KSQ|rcsb pdb:4FC0|rcsb pdb:4MBJ|rcsb pdb:4MNE|rcsb pdb:4MNF|rcsb pdb:4PP7|rcsb pdb:4R5Y|rcsb pdb:4RZV|rcsb pdb:4RZW|rcsb pdb:4WO5|rcsb pdb:4XV1|rcsb pdb:4XV2|rcsb pdb:4XV3|rcsb pdb:4XV9|rcsb pdb:4YHT|rcsb pdb:5C9C|rcsb pdb:5CSW|rcsb pdb:5CSX|rcsb pdb:5CT7|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0070374"(positive regulation of ERK1 and ERK2 cascade)|go:"GO:0043066"(negative regulation of apoptotic process)|go:"GO:0070413"(trehalose metabolism in response to stress)|go:"GO:0071277"(cellular response to calcium ion)|go:"GO:0090150"(establishment of protein localization to membrane)|go:"GO:0097110"(scaffold protein binding)|go:"GO:0106310"(protein serine kinase activity)|go:"GO:0106311"|interpro:IPR000719(Protein kinase, core)|interpro:IPR001245(Tyrosine protein kinase)|interpro:IPR002219(Protein kinase C, phorbol ester/diacylglycerol binding)|interpro:IPR003116(Raf-like Ras-binding)|interpro:IPR008271(Serine/threonine protein kinase, active site)|interpro:IPR011009(Protein kinase-like)|interpro:IPR017441(Protein kinase ATP binding, conserved site)|interpro:IPR020454|rcsb pdb:5FD2|rcsb pdb:5HI2|rcsb pdb:5HID|rcsb pdb:5HIE|rcsb pdb:5ITA|rcsb pdb:5J17|rcsb pdb:5J18|rcsb pdb:5J2R|rcsb pdb:5JRQ|rcsb pdb:5JSM|rcsb pdb:5JT2|rcsb pdb:5VAL|rcsb pdb:5VAM|rcsb pdb:5VR3|rcsb pdb:5VYK|rcsb pdb:6B8U|rcsb pdb:6CAD|rcsb pdb:6N0P|rcsb pdb:6N0Q|rcsb pdb:6NSQ|rcsb pdb:6NYB|rcsb pdb:6P3D|rcsb pdb:6P7G|rcsb pdb:6PP9|rcsb pdb:6Q0J|rcsb pdb:6Q0K|rcsb pdb:6Q0T|rcsb pdb:6U2G|rcsb pdb:6U2H|rcsb pdb:6UAN|rcsb pdb:6UUO|rcsb pdb:6V2U|rcsb pdb:6V2W|rcsb pdb:6XAG|mint:P15056|rcsb pdb:1UWH|rcsb pdb:1UWJ|rcsb pdb:2FB8|rcsb pdb:2L05|rcsb pdb:3C4C|interpro:IPR029071|rcsb pdb:3D4Q|rcsb pdb:3IDP|rcsb pdb:3II5|rcsb pdb:3NY5|rcsb pdb:3OG7|rcsb pdb:3PPJ|rcsb pdb:3PPK|rcsb pdb:3PRF|rcsb pdb:3PRI|rcsb pdb:3PSB|rcsb pdb:3PSD|rcsb pdb:3Q4C|rcsb pdb:3Q96|rcsb pdb:3SKC|rcsb pdb:3TV4|rcsb pdb:3TV6|rcsb pdb:4CQE|rcsb pdb:4DBN|rcsb pdb:4E26|rcsb pdb:4E4X|rcsb pdb:4EHE|rcsb pdb:4EHG|refseq:NP_004324.2|ensembl:ENSP00000493543|dip:DIP-1045N	-	-	-	figure legend:6D|agonist:The MEK inhibitor  PD0325901|antagonist:Trametinib - a newer MEK inhibitor|dataset:Cancer - Interactions investigated in the context of cancer|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:9606(human-a_549)|taxid:9606(Homo sapiens Caucasian lung carcinoma cell line)	-	2014/06/12	2015/05/22	rogid:ieBLcDINn9hZFZZEIQ3Ktdf2NF89606	rogid:sfzILpNpX8UFB/vgH9LOKLpl/+g9606	rigid:BpXg3tpBH0abj15rihKRcuuAOPk	false	-	-	-	-	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)
uniprotkb:Q02750	uniprotkb:P15056	intact:EBI-492564|ensembl:ENSP00000302486	intact:EBI-365980|uniprotkb:Q13878|uniprotkb:Q3MIN6|intact:EBI-28988924|ensembl:ENSP00000493543|uniprotkb:Q9UDP8|uniprotkb:Q9Y6T3|uniprotkb:A4D1T4|uniprotkb:B6HY61|uniprotkb:B6HY62|uniprotkb:B6HY63|uniprotkb:B6HY64|uniprotkb:B6HY65|uniprotkb:B6HY66	psi-mi:mp2k1_human(display_long)|uniprotkb:ERK activator kinase 1(gene name synonym)|uniprotkb:MAPK/ERK kinase 1(gene name synonym)|uniprotkb:MAP2K1(gene name)|psi-mi:MAP2K1(display_short)|uniprotkb:MEK1(gene name synonym)|uniprotkb:PRKMK1(gene name synonym)	psi-mi:braf_human(display_long)|uniprotkb:BRAF(gene name)|psi-mi:BRAF(display_short)|uniprotkb:BRAF1(gene name synonym)|uniprotkb:RAFB1(gene name synonym)|uniprotkb:v-Raf murine sarcoma viral oncogene homolog B1(gene name synonym)|uniprotkb:p94(gene name synonym)|uniprotkb:Proto-oncogene B-Raf(gene name synonym)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Lito et al. (2014)	pubmed:24746704|imex:IM-22983	taxid:9606(human)|taxid:9606(Homo sapiens)	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-10823668|imex:IM-22983-13	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_002746.1|refseq:XP_016877900.1|dip:DIP-201N|ensembl:ENSG00000169032(gene)|ensembl:ENST00000307102(transcript)|go:"GO:0000187"(obsolete activation of MAPK activity)|go:"GO:0004672"(protein kinase activity)|go:"GO:0004674"(protein serine/threonine kinase activity)|go:"GO:0004708"(MAP kinase kinase activity)|go:"GO:0004712"(protein serine/threonine/tyrosine kinase activity)|go:"GO:0004714"(transmembrane receptor protein tyrosine kinase activity)|go:"GO:0005078"(MAP-kinase scaffold activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005634"(nucleus)|go:"GO:0005739"(mitochondrion)|go:"GO:0005769"(early endosome)|go:"GO:0005770"(late endosome)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005815"(microtubule organizing center)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0005925"(focal adhesion)|go:"GO:0006468"(protein phosphorylation)|go:"GO:0006935"(chemotaxis)|go:"GO:0007165"(signal transduction)|go:"GO:0007507"(heart development)|go:"GO:0008022"(protein C-terminus binding)|go:"GO:0008285"(negative regulation of cell population proliferation)|go:"GO:0010628"(positive regulation of gene expression)|go:"GO:0018107"(peptidyl-threonine phosphorylation)|go:"GO:0021697"(cerebellar cortex formation)|go:"GO:0030182"(neuron differentiation)|go:"GO:0030216"(keratinocyte differentiation)|go:"GO:0030878"(thyroid gland development)|go:"GO:0032872"(regulation of stress-activated MAPK cascade)|go:"GO:0043539"(protein serine/threonine kinase activator activity)|go:"GO:0045893"(positive regulation of transcription, DNA-templated)|go:"GO:0047485"(protein N-terminus binding)|go:"GO:0048538"(thymus development)|go:"GO:0048679"(regulation of axon regeneration)|go:"GO:0048870"(cell motility)|go:"GO:0050772"(positive regulation of axonogenesis)|go:"GO:0051726"(regulation of cell cycle)|go:"GO:0060020"(Bergmann glial cell differentiation)|go:"GO:0060324"(face development)|go:"GO:0060440"(trachea formation)|go:"GO:0060502"(epithelial cell proliferation involved in lung morphogenesis)|go:"GO:0060674"(placenta blood vessel development)|go:"GO:0060711"(labyrinthine layer development)|go:"GO:0070371"(ERK1 and ERK2 cascade)|go:"GO:0070374"(positive regulation of ERK1 and ERK2 cascade)|go:"GO:0071902"(positive regulation of protein serine/threonine kinase activity)|go:"GO:0090170"(regulation of Golgi inheritance)|go:"GO:0090398"(cellular senescence)|go:"GO:0097110"(scaffold protein binding)|go:"GO:0106310"(protein serine kinase activity)|go:"GO:0106311"|go:"GO:1903800"(positive regulation of production of miRNAs involved in gene silencing by miRNA)|go:"GO:2000641"(regulation of early endosome to late endosome transport)|interpro:IPR000719(Protein kinase, core)|interpro:IPR008271(Serine/threonine protein kinase, active site)|interpro:IPR011009(Protein kinase-like)|interpro:IPR017441(Protein kinase ATP binding, conserved site)|mint:Q02750|rcsb pdb:1S9J|rcsb pdb:2P55|rcsb pdb:3DV3|rcsb pdb:3DY7|rcsb pdb:3E8N|rcsb pdb:3EQB|rcsb pdb:3EQC|rcsb pdb:3EQD|rcsb pdb:3EQF|rcsb pdb:3EQG|rcsb pdb:3EQH|rcsb pdb:3EQI|rcsb pdb:3MBL|rcsb pdb:3ORN|rcsb pdb:3OS3|rcsb pdb:3PP1|rcsb pdb:3SLS|rcsb pdb:3V01|rcsb pdb:3V04|rcsb pdb:3VVH|rcsb pdb:3W8Q|rcsb pdb:3WIG|rcsb pdb:3ZLS|rcsb pdb:3ZLW|rcsb pdb:3ZLX|rcsb pdb:3ZLY|rcsb pdb:3ZM4|rcsb pdb:4AN2|rcsb pdb:4AN3|rcsb pdb:4AN9|rcsb pdb:4ANB|rcsb pdb:4ARK|rcsb pdb:4LMN|rcsb pdb:4MNE|rcsb pdb:4U7Z|rcsb pdb:4U80|rcsb pdb:4U81|rcsb pdb:5EYM|rcsb pdb:5HZE|rcsb pdb:5YT3|rcsb pdb:6NYB|rcsb pdb:6PP9|rcsb pdb:6Q0J|rcsb pdb:6Q0T|rcsb pdb:6U2G|rcsb pdb:6V2W|rcsb pdb:6X2P|rcsb pdb:6X2S|rcsb pdb:6X2X|rcsb pdb:7B3M|rcsb pdb:7B7R|rcsb pdb:7B94|rcsb pdb:7B9L|rcsb pdb:7M0T|rcsb pdb:7M0U|rcsb pdb:7M0V|rcsb pdb:7M0W|rcsb pdb:7M0X|rcsb pdb:7M0Y|rcsb pdb:7M0Z|reactome:R-HSA-110056|reactome:R-HSA-170968|reactome:R-HSA-445144|reactome:R-HSA-5210891|reactome:R-HSA-5673000|reactome:R-HSA-5674135|reactome:R-HSA-5674499|reactome:R-HSA-5684264|reactome:R-HSA-6802946|reactome:R-HSA-6802948|reactome:R-HSA-6802952|reactome:R-HSA-6802955|reactome:R-HSA-9649948|reactome:R-HSA-9652169|reactome:R-HSA-9656223|rcsb pdb:5BX0	ensembl:ENSG00000157764(gene)|ensembl:ENST00000646891(transcript)|go:"GO:0000165"(MAPK cascade)|go:"GO:0004672"(protein kinase activity)|go:"GO:0004674"(protein serine/threonine kinase activity)|go:"GO:0004708"(MAP kinase kinase activity)|go:"GO:0005509"(calcium ion binding)|go:"GO:0005524"(ATP binding)|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0006468"(protein phosphorylation)|go:"GO:0007173"(epidermal growth factor receptor signaling pathway)|go:"GO:0009887"(animal organ morphogenesis)|go:"GO:0010628"(positive regulation of gene expression)|go:"GO:0010828"(positive regulation of glucose transmembrane transport)|go:"GO:0033138"(positive regulation of peptidyl-serine phosphorylation)|rcsb pdb:7M0T|rcsb pdb:7M0U|rcsb pdb:7M0V|go:"GO:0042802"(identical protein binding)|rcsb pdb:7M0W|rcsb pdb:7M0X|rcsb pdb:7M0Y|rcsb pdb:7M0Z|reactome:R-HSA-1295596|reactome:R-HSA-170968|reactome:R-HSA-170984|reactome:R-HSA-187706|reactome:R-HSA-5673000|reactome:R-HSA-5674135|reactome:R-HSA-5674499|reactome:R-HSA-5675221|reactome:R-HSA-6802946|reactome:R-HSA-6802948|reactome:R-HSA-6802952|reactome:R-HSA-6802955|reactome:R-HSA-9649948|reactome:R-HSA-9656223|reactome:R-HSA-9660537|rcsb pdb:4FK3|rcsb pdb:4G9C|rcsb pdb:4G9R|rcsb pdb:4H58|rcsb pdb:4JVG|rcsb pdb:4KSP|rcsb pdb:4KSQ|rcsb pdb:4FC0|rcsb pdb:4MBJ|rcsb pdb:4MNE|rcsb pdb:4MNF|rcsb pdb:4PP7|rcsb pdb:4R5Y|rcsb pdb:4RZV|rcsb pdb:4RZW|rcsb pdb:4WO5|rcsb pdb:4XV1|rcsb pdb:4XV2|rcsb pdb:4XV3|rcsb pdb:4XV9|rcsb pdb:4YHT|rcsb pdb:5C9C|rcsb pdb:5CSW|rcsb pdb:5CSX|rcsb pdb:5CT7|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0070374"(positive regulation of ERK1 and ERK2 cascade)|go:"GO:0043066"(negative regulation of apoptotic process)|go:"GO:0070413"(trehalose metabolism in response to stress)|go:"GO:0071277"(cellular response to calcium ion)|go:"GO:0090150"(establishment of protein localization to membrane)|go:"GO:0097110"(scaffold protein binding)|go:"GO:0106310"(protein serine kinase activity)|go:"GO:0106311"|interpro:IPR000719(Protein kinase, core)|interpro:IPR001245(Tyrosine protein kinase)|interpro:IPR002219(Protein kinase C, phorbol ester/diacylglycerol binding)|interpro:IPR003116(Raf-like Ras-binding)|interpro:IPR008271(Serine/threonine protein kinase, active site)|interpro:IPR011009(Protein kinase-like)|interpro:IPR017441(Protein kinase ATP binding, conserved site)|interpro:IPR020454|rcsb pdb:5FD2|rcsb pdb:5HI2|rcsb pdb:5HID|rcsb pdb:5HIE|rcsb pdb:5ITA|rcsb pdb:5J17|rcsb pdb:5J18|rcsb pdb:5J2R|rcsb pdb:5JRQ|rcsb pdb:5JSM|rcsb pdb:5JT2|rcsb pdb:5VAL|rcsb pdb:5VAM|rcsb pdb:5VR3|rcsb pdb:5VYK|rcsb pdb:6B8U|rcsb pdb:6CAD|rcsb pdb:6N0P|rcsb pdb:6N0Q|rcsb pdb:6NSQ|rcsb pdb:6NYB|rcsb pdb:6P3D|rcsb pdb:6P7G|rcsb pdb:6PP9|rcsb pdb:6Q0J|rcsb pdb:6Q0K|rcsb pdb:6Q0T|rcsb pdb:6U2G|rcsb pdb:6U2H|rcsb pdb:6UAN|rcsb pdb:6UUO|rcsb pdb:6V2U|rcsb pdb:6V2W|rcsb pdb:6XAG|mint:P15056|rcsb pdb:1UWH|rcsb pdb:1UWJ|rcsb pdb:2FB8|rcsb pdb:2L05|rcsb pdb:3C4C|interpro:IPR029071|rcsb pdb:3D4Q|rcsb pdb:3IDP|rcsb pdb:3II5|rcsb pdb:3NY5|rcsb pdb:3OG7|rcsb pdb:3PPJ|rcsb pdb:3PPK|rcsb pdb:3PRF|rcsb pdb:3PRI|rcsb pdb:3PSB|rcsb pdb:3PSD|rcsb pdb:3Q4C|rcsb pdb:3Q96|rcsb pdb:3SKC|rcsb pdb:3TV4|rcsb pdb:3TV6|rcsb pdb:4CQE|rcsb pdb:4DBN|rcsb pdb:4E26|rcsb pdb:4E4X|rcsb pdb:4EHE|rcsb pdb:4EHG|refseq:NP_004324.2|ensembl:ENSP00000493543|dip:DIP-1045N	-	-	-	figure legend:6D|agonist:The MEK inhibitor  PD0325901|antagonist:Trametinib - a newer MEK inhibitor|comment:weak interaction|variable_condition:"Time of Trametinib treatment (hours)-3"|dataset:Cancer - Interactions investigated in the context of cancer|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:9606(human-a_549)|taxid:9606(Homo sapiens Caucasian lung carcinoma cell line)	-	2014/06/12	2015/05/22	rogid:ieBLcDINn9hZFZZEIQ3Ktdf2NF89606	rogid:sfzILpNpX8UFB/vgH9LOKLpl/+g9606	rigid:BpXg3tpBH0abj15rihKRcuuAOPk	false	-	-	-	-	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)
uniprotkb:Q02750	uniprotkb:P04049	intact:EBI-492564|ensembl:ENSP00000302486	intact:EBI-365996|uniprotkb:B0LPH8|uniprotkb:Q15278|uniprotkb:Q9UC20|ensembl:ENSP00000251849|uniprotkb:B2R5N3	psi-mi:mp2k1_human(display_long)|uniprotkb:ERK activator kinase 1(gene name synonym)|uniprotkb:MAPK/ERK kinase 1(gene name synonym)|uniprotkb:MAP2K1(gene name)|psi-mi:MAP2K1(display_short)|uniprotkb:MEK1(gene name synonym)|uniprotkb:PRKMK1(gene name synonym)	psi-mi:raf1_human(display_long)|uniprotkb:RAF1(gene name)|psi-mi:RAF1(display_short)|uniprotkb:RAF(gene name synonym)|uniprotkb:Proto-oncogene c-RAF(gene name synonym)|uniprotkb:Raf-1(gene name synonym)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Lito et al. (2014)	pubmed:24746704|imex:IM-22983	taxid:9606(human)|taxid:9606(Homo sapiens)	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0914"(association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-10823668|imex:IM-22983-13	-	psi-mi:"MI:1060"(spoke expansion)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_002746.1|refseq:XP_016877900.1|dip:DIP-201N|ensembl:ENSG00000169032(gene)|ensembl:ENST00000307102(transcript)|go:"GO:0000187"(obsolete activation of MAPK activity)|go:"GO:0004672"(protein kinase activity)|go:"GO:0004674"(protein serine/threonine kinase activity)|go:"GO:0004708"(MAP kinase kinase activity)|go:"GO:0004712"(protein serine/threonine/tyrosine kinase activity)|go:"GO:0004714"(transmembrane receptor protein tyrosine kinase activity)|go:"GO:0005078"(MAP-kinase scaffold activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005634"(nucleus)|go:"GO:0005739"(mitochondrion)|go:"GO:0005769"(early endosome)|go:"GO:0005770"(late endosome)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005815"(microtubule organizing center)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0005925"(focal adhesion)|go:"GO:0006468"(protein phosphorylation)|go:"GO:0006935"(chemotaxis)|go:"GO:0007165"(signal transduction)|go:"GO:0007507"(heart development)|go:"GO:0008022"(protein C-terminus binding)|go:"GO:0008285"(negative regulation of cell population proliferation)|go:"GO:0010628"(positive regulation of gene expression)|go:"GO:0018107"(peptidyl-threonine phosphorylation)|go:"GO:0021697"(cerebellar cortex formation)|go:"GO:0030182"(neuron differentiation)|go:"GO:0030216"(keratinocyte differentiation)|go:"GO:0030878"(thyroid gland development)|go:"GO:0032872"(regulation of stress-activated MAPK cascade)|go:"GO:0043539"(protein serine/threonine kinase activator activity)|go:"GO:0045893"(positive regulation of transcription, DNA-templated)|go:"GO:0047485"(protein N-terminus binding)|go:"GO:0048538"(thymus development)|go:"GO:0048679"(regulation of axon regeneration)|go:"GO:0048870"(cell motility)|go:"GO:0050772"(positive regulation of axonogenesis)|go:"GO:0051726"(regulation of cell cycle)|go:"GO:0060020"(Bergmann glial cell differentiation)|go:"GO:0060324"(face development)|go:"GO:0060440"(trachea formation)|go:"GO:0060502"(epithelial cell proliferation involved in lung morphogenesis)|go:"GO:0060674"(placenta blood vessel development)|go:"GO:0060711"(labyrinthine layer development)|go:"GO:0070371"(ERK1 and ERK2 cascade)|go:"GO:0070374"(positive regulation of ERK1 and ERK2 cascade)|go:"GO:0071902"(positive regulation of protein serine/threonine kinase activity)|go:"GO:0090170"(regulation of Golgi inheritance)|go:"GO:0090398"(cellular senescence)|go:"GO:0097110"(scaffold protein binding)|go:"GO:0106310"(protein serine kinase activity)|go:"GO:0106311"|go:"GO:1903800"(positive regulation of production of miRNAs involved in gene silencing by miRNA)|go:"GO:2000641"(regulation of early endosome to late endosome transport)|interpro:IPR000719(Protein kinase, core)|interpro:IPR008271(Serine/threonine protein kinase, active site)|interpro:IPR011009(Protein kinase-like)|interpro:IPR017441(Protein kinase ATP binding, conserved site)|mint:Q02750|rcsb pdb:1S9J|rcsb pdb:2P55|rcsb pdb:3DV3|rcsb pdb:3DY7|rcsb pdb:3E8N|rcsb pdb:3EQB|rcsb pdb:3EQC|rcsb pdb:3EQD|rcsb pdb:3EQF|rcsb pdb:3EQG|rcsb pdb:3EQH|rcsb pdb:3EQI|rcsb pdb:3MBL|rcsb pdb:3ORN|rcsb pdb:3OS3|rcsb pdb:3PP1|rcsb pdb:3SLS|rcsb pdb:3V01|rcsb pdb:3V04|rcsb pdb:3VVH|rcsb pdb:3W8Q|rcsb pdb:3WIG|rcsb pdb:3ZLS|rcsb pdb:3ZLW|rcsb pdb:3ZLX|rcsb pdb:3ZLY|rcsb pdb:3ZM4|rcsb pdb:4AN2|rcsb pdb:4AN3|rcsb pdb:4AN9|rcsb pdb:4ANB|rcsb pdb:4ARK|rcsb pdb:4LMN|rcsb pdb:4MNE|rcsb pdb:4U7Z|rcsb pdb:4U80|rcsb pdb:4U81|rcsb pdb:5EYM|rcsb pdb:5HZE|rcsb pdb:5YT3|rcsb pdb:6NYB|rcsb pdb:6PP9|rcsb pdb:6Q0J|rcsb pdb:6Q0T|rcsb pdb:6U2G|rcsb pdb:6V2W|rcsb pdb:6X2P|rcsb pdb:6X2S|rcsb pdb:6X2X|rcsb pdb:7B3M|rcsb pdb:7B7R|rcsb pdb:7B94|rcsb pdb:7B9L|rcsb pdb:7M0T|rcsb pdb:7M0U|rcsb pdb:7M0V|rcsb pdb:7M0W|rcsb pdb:7M0X|rcsb pdb:7M0Y|rcsb pdb:7M0Z|reactome:R-HSA-110056|reactome:R-HSA-170968|reactome:R-HSA-445144|reactome:R-HSA-5210891|reactome:R-HSA-5673000|reactome:R-HSA-5674135|reactome:R-HSA-5674499|reactome:R-HSA-5684264|reactome:R-HSA-6802946|reactome:R-HSA-6802948|reactome:R-HSA-6802952|reactome:R-HSA-6802955|reactome:R-HSA-9649948|reactome:R-HSA-9652169|reactome:R-HSA-9656223|rcsb pdb:5BX0	rcsb pdb:3IQV|rcsb pdb:3KUC|rcsb pdb:3KUD|rcsb pdb:3NKX|rcsb pdb:3O8I|rcsb pdb:3OMV|rcsb pdb:4FJ3|rcsb pdb:4G0N|rcsb pdb:4G3X|rcsb pdb:4IEA|rcsb pdb:4IHL|rcsb pdb:6NTC|rcsb pdb:6NTD|rcsb pdb:6PTS|rcsb pdb:6PTW|rcsb pdb:6VJJ|rcsb pdb:6XGU|rcsb pdb:6XGV|rcsb pdb:6XHA|rcsb pdb:6XHB|rcsb pdb:6XI7|ensembl:ENSG00000132155(gene)|ensembl:ENST00000251849(transcript)|go:"GO:0000186"(obsolete activation of MAPKK activity)|go:"GO:0001666"(response to hypoxia)|go:"GO:0004672"(protein kinase activity)|go:"GO:0004674"(protein serine/threonine kinase activity)|go:"GO:0004709"(MAP kinase kinase kinase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005737"(cytoplasm)|go:"GO:0005739"(mitochondrion)|go:"GO:0005741"(mitochondrial outer membrane)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0006468"(protein phosphorylation)|go:"GO:0006915"(apoptotic process)|go:"GO:0007165"(signal transduction)|go:"GO:0007190"(activation of adenylate cyclase activity)|go:"GO:0007507"(heart development)|go:"GO:0008179"(adenylate cyclase binding)|go:"GO:0008285"(negative regulation of cell population proliferation)|go:"GO:0010856"(adenylate cyclase activator activity)|go:"GO:0016607"(nuclear speck)|go:"GO:0019899"(enzyme binding)|go:"GO:0030154"(cell differentiation)|go:"GO:0030878"(thyroid gland development)|go:"GO:0031143"(pseudopodium)|go:"GO:0031333"(negative regulation of protein-containing complex assembly)|go:"GO:0031434"(mitogen-activated protein kinase kinase binding)|go:"GO:0033138"(positive regulation of peptidyl-serine phosphorylation)|go:"GO:0035019"(somatic stem cell population maintenance)|go:"GO:0035023"(regulation of Rho protein signal transduction)|go:"GO:0035773"(insulin secretion involved in cellular response to glucose stimulus)|go:"GO:0035994"(response to muscle stretch)|go:"GO:0042060"(wound healing)|go:"GO:0042802"(identical protein binding)|go:"GO:0042981"(regulation of apoptotic process)|go:"GO:0043066"(negative regulation of apoptotic process)|go:"GO:0043154"(negative regulation of cysteine-type endopeptidase activity involved in apoptotic process)|go:"GO:0044877"(protein-containing complex binding)|go:"GO:0045104"(intermediate filament cytoskeleton organization)|go:"GO:0045595"(regulation of cell differentiation)|go:"GO:0045944"(positive regulation of transcription by RNA polymerase II)|go:"GO:0046872"(metal ion binding)|go:"GO:0048011"(neurotrophin TRK receptor signaling pathway)|go:"GO:0048538"(thymus development)|go:"GO:0060324"(face development)|go:"GO:0071550"(death-inducing signaling complex assembly)|go:"GO:0106310"(protein serine kinase activity)|go:"GO:0106311"|go:"GO:1902042"(negative regulation of extrinsic apoptotic signaling pathway via death domain receptors)|go:"GO:2000145"(regulation of cell motility)|interpro:IPR000719(Protein kinase, core)|interpro:IPR001245(Tyrosine protein kinase)|interpro:IPR002219(Protein kinase C, phorbol ester/diacylglycerol binding)|interpro:IPR003116(Raf-like Ras-binding)|interpro:IPR008271(Serine/threonine protein kinase, active site)|interpro:IPR011009(Protein kinase-like)|interpro:IPR017441(Protein kinase ATP binding, conserved site)|interpro:IPR020454|interpro:IPR029071|mint:P04049|rcsb pdb:1C1Y|rcsb pdb:1FAQ|rcsb pdb:1FAR|rcsb pdb:1GUA|rcsb pdb:1RFA|rcsb pdb:3CU8|rcsb pdb:3IQJ|rcsb pdb:3IQU|rcsb pdb:7JHP|reactome:R-HSA-2672351|reactome:R-HSA-392517|reactome:R-HSA-430116|reactome:R-HSA-5621575|reactome:R-HSA-5673000|reactome:R-HSA-5674135|reactome:R-HSA-5674499|reactome:R-HSA-5675221|reactome:R-HSA-6802946|reactome:R-HSA-6802948|reactome:R-HSA-6802952|reactome:R-HSA-6802955|reactome:R-HSA-9649948|reactome:R-HSA-9656223|reactome:R-HSA-9660537|refseq:NP_002871.1|refseq:XP_011532276.1|refseq:XP_005265412.1|dip:DIP-1048N	-	-	-	figure legend:6D|agonist:The MEK inhibitor  PD0325901|antagonist:Trametinib - a newer MEK inhibitor|comment:weak interaction|variable_condition:"Time of Trametinib treatment (hours)-3"|dataset:Cancer - Interactions investigated in the context of cancer|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:9606(human-a_549)|taxid:9606(Homo sapiens Caucasian lung carcinoma cell line)	-	2014/06/12	2015/05/22	rogid:ieBLcDINn9hZFZZEIQ3Ktdf2NF89606	rogid:1WOAPuzcZdMoq9hyFD/qr4Tz7049606	rigid:BpXg3tpBH0abj15rihKRcuuAOPk	false	-	-	-	-	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)
uniprotkb:P15056	uniprotkb:Q02750	intact:EBI-365980|uniprotkb:Q13878|uniprotkb:Q3MIN6|intact:EBI-28988924|ensembl:ENSP00000493543|uniprotkb:Q9UDP8|uniprotkb:Q9Y6T3|uniprotkb:A4D1T4|uniprotkb:B6HY61|uniprotkb:B6HY62|uniprotkb:B6HY63|uniprotkb:B6HY64|uniprotkb:B6HY65|uniprotkb:B6HY66	intact:EBI-492564|ensembl:ENSP00000302486	psi-mi:braf_human(display_long)|uniprotkb:BRAF(gene name)|psi-mi:BRAF(display_short)|uniprotkb:BRAF1(gene name synonym)|uniprotkb:RAFB1(gene name synonym)|uniprotkb:v-Raf murine sarcoma viral oncogene homolog B1(gene name synonym)|uniprotkb:p94(gene name synonym)|uniprotkb:Proto-oncogene B-Raf(gene name synonym)	psi-mi:mp2k1_human(display_long)|uniprotkb:ERK activator kinase 1(gene name synonym)|uniprotkb:MAPK/ERK kinase 1(gene name synonym)|uniprotkb:MAP2K1(gene name)|psi-mi:MAP2K1(display_short)|uniprotkb:MEK1(gene name synonym)|uniprotkb:PRKMK1(gene name synonym)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Lito et al. (2014)	pubmed:24746704|imex:IM-22983	taxid:9606(human)|taxid:9606(Homo sapiens)	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-10823661|imex:IM-22983-11	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	ensembl:ENSG00000157764(gene)|ensembl:ENST00000646891(transcript)|go:"GO:0000165"(MAPK cascade)|go:"GO:0004672"(protein kinase activity)|go:"GO:0004674"(protein serine/threonine kinase activity)|go:"GO:0004708"(MAP kinase kinase activity)|go:"GO:0005509"(calcium ion binding)|go:"GO:0005524"(ATP binding)|go:"GO:0005634"(nucleus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0006468"(protein phosphorylation)|go:"GO:0007173"(epidermal growth factor receptor signaling pathway)|go:"GO:0009887"(animal organ morphogenesis)|go:"GO:0010628"(positive regulation of gene expression)|go:"GO:0010828"(positive regulation of glucose transmembrane transport)|go:"GO:0033138"(positive regulation of peptidyl-serine phosphorylation)|rcsb pdb:7M0T|rcsb pdb:7M0U|rcsb pdb:7M0V|go:"GO:0042802"(identical protein binding)|rcsb pdb:7M0W|rcsb pdb:7M0X|rcsb pdb:7M0Y|rcsb pdb:7M0Z|reactome:R-HSA-1295596|reactome:R-HSA-170968|reactome:R-HSA-170984|reactome:R-HSA-187706|reactome:R-HSA-5673000|reactome:R-HSA-5674135|reactome:R-HSA-5674499|reactome:R-HSA-5675221|reactome:R-HSA-6802946|reactome:R-HSA-6802948|reactome:R-HSA-6802952|reactome:R-HSA-6802955|reactome:R-HSA-9649948|reactome:R-HSA-9656223|reactome:R-HSA-9660537|rcsb pdb:4FK3|rcsb pdb:4G9C|rcsb pdb:4G9R|rcsb pdb:4H58|rcsb pdb:4JVG|rcsb pdb:4KSP|rcsb pdb:4KSQ|rcsb pdb:4FC0|rcsb pdb:4MBJ|rcsb pdb:4MNE|rcsb pdb:4MNF|rcsb pdb:4PP7|rcsb pdb:4R5Y|rcsb pdb:4RZV|rcsb pdb:4RZW|rcsb pdb:4WO5|rcsb pdb:4XV1|rcsb pdb:4XV2|rcsb pdb:4XV3|rcsb pdb:4XV9|rcsb pdb:4YHT|rcsb pdb:5C9C|rcsb pdb:5CSW|rcsb pdb:5CSX|rcsb pdb:5CT7|go:"GO:0043231"(intracellular membrane-bounded organelle)|go:"GO:0070374"(positive regulation of ERK1 and ERK2 cascade)|go:"GO:0043066"(negative regulation of apoptotic process)|go:"GO:0070413"(trehalose metabolism in response to stress)|go:"GO:0071277"(cellular response to calcium ion)|go:"GO:0090150"(establishment of protein localization to membrane)|go:"GO:0097110"(scaffold protein binding)|go:"GO:0106310"(protein serine kinase activity)|go:"GO:0106311"|interpro:IPR000719(Protein kinase, core)|interpro:IPR001245(Tyrosine protein kinase)|interpro:IPR002219(Protein kinase C, phorbol ester/diacylglycerol binding)|interpro:IPR003116(Raf-like Ras-binding)|interpro:IPR008271(Serine/threonine protein kinase, active site)|interpro:IPR011009(Protein kinase-like)|interpro:IPR017441(Protein kinase ATP binding, conserved site)|interpro:IPR020454|rcsb pdb:5FD2|rcsb pdb:5HI2|rcsb pdb:5HID|rcsb pdb:5HIE|rcsb pdb:5ITA|rcsb pdb:5J17|rcsb pdb:5J18|rcsb pdb:5J2R|rcsb pdb:5JRQ|rcsb pdb:5JSM|rcsb pdb:5JT2|rcsb pdb:5VAL|rcsb pdb:5VAM|rcsb pdb:5VR3|rcsb pdb:5VYK|rcsb pdb:6B8U|rcsb pdb:6CAD|rcsb pdb:6N0P|rcsb pdb:6N0Q|rcsb pdb:6NSQ|rcsb pdb:6NYB|rcsb pdb:6P3D|rcsb pdb:6P7G|rcsb pdb:6PP9|rcsb pdb:6Q0J|rcsb pdb:6Q0K|rcsb pdb:6Q0T|rcsb pdb:6U2G|rcsb pdb:6U2H|rcsb pdb:6UAN|rcsb pdb:6UUO|rcsb pdb:6V2U|rcsb pdb:6V2W|rcsb pdb:6XAG|mint:P15056|rcsb pdb:1UWH|rcsb pdb:1UWJ|rcsb pdb:2FB8|rcsb pdb:2L05|rcsb pdb:3C4C|interpro:IPR029071|rcsb pdb:3D4Q|rcsb pdb:3IDP|rcsb pdb:3II5|rcsb pdb:3NY5|rcsb pdb:3OG7|rcsb pdb:3PPJ|rcsb pdb:3PPK|rcsb pdb:3PRF|rcsb pdb:3PRI|rcsb pdb:3PSB|rcsb pdb:3PSD|rcsb pdb:3Q4C|rcsb pdb:3Q96|rcsb pdb:3SKC|rcsb pdb:3TV4|rcsb pdb:3TV6|rcsb pdb:4CQE|rcsb pdb:4DBN|rcsb pdb:4E26|rcsb pdb:4E4X|rcsb pdb:4EHE|rcsb pdb:4EHG|refseq:NP_004324.2|ensembl:ENSP00000493543|dip:DIP-1045N	refseq:NP_002746.1|refseq:XP_016877900.1|dip:DIP-201N|ensembl:ENSG00000169032(gene)|ensembl:ENST00000307102(transcript)|go:"GO:0000187"(obsolete activation of MAPK activity)|go:"GO:0004672"(protein kinase activity)|go:"GO:0004674"(protein serine/threonine kinase activity)|go:"GO:0004708"(MAP kinase kinase activity)|go:"GO:0004712"(protein serine/threonine/tyrosine kinase activity)|go:"GO:0004714"(transmembrane receptor protein tyrosine kinase activity)|go:"GO:0005078"(MAP-kinase scaffold activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005634"(nucleus)|go:"GO:0005739"(mitochondrion)|go:"GO:0005769"(early endosome)|go:"GO:0005770"(late endosome)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005815"(microtubule organizing center)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0005925"(focal adhesion)|go:"GO:0006468"(protein phosphorylation)|go:"GO:0006935"(chemotaxis)|go:"GO:0007165"(signal transduction)|go:"GO:0007507"(heart development)|go:"GO:0008022"(protein C-terminus binding)|go:"GO:0008285"(negative regulation of cell population proliferation)|go:"GO:0010628"(positive regulation of gene expression)|go:"GO:0018107"(peptidyl-threonine phosphorylation)|go:"GO:0021697"(cerebellar cortex formation)|go:"GO:0030182"(neuron differentiation)|go:"GO:0030216"(keratinocyte differentiation)|go:"GO:0030878"(thyroid gland development)|go:"GO:0032872"(regulation of stress-activated MAPK cascade)|go:"GO:0043539"(protein serine/threonine kinase activator activity)|go:"GO:0045893"(positive regulation of transcription, DNA-templated)|go:"GO:0047485"(protein N-terminus binding)|go:"GO:0048538"(thymus development)|go:"GO:0048679"(regulation of axon regeneration)|go:"GO:0048870"(cell motility)|go:"GO:0050772"(positive regulation of axonogenesis)|go:"GO:0051726"(regulation of cell cycle)|go:"GO:0060020"(Bergmann glial cell differentiation)|go:"GO:0060324"(face development)|go:"GO:0060440"(trachea formation)|go:"GO:0060502"(epithelial cell proliferation involved in lung morphogenesis)|go:"GO:0060674"(placenta blood vessel development)|go:"GO:0060711"(labyrinthine layer development)|go:"GO:0070371"(ERK1 and ERK2 cascade)|go:"GO:0070374"(positive regulation of ERK1 and ERK2 cascade)|go:"GO:0071902"(positive regulation of protein serine/threonine kinase activity)|go:"GO:0090170"(regulation of Golgi inheritance)|go:"GO:0090398"(cellular senescence)|go:"GO:0097110"(scaffold protein binding)|go:"GO:0106310"(protein serine kinase activity)|go:"GO:0106311"|go:"GO:1903800"(positive regulation of production of miRNAs involved in gene silencing by miRNA)|go:"GO:2000641"(regulation of early endosome to late endosome transport)|interpro:IPR000719(Protein kinase, core)|interpro:IPR008271(Serine/threonine protein kinase, active site)|interpro:IPR011009(Protein kinase-like)|interpro:IPR017441(Protein kinase ATP binding, conserved site)|mint:Q02750|rcsb pdb:1S9J|rcsb pdb:2P55|rcsb pdb:3DV3|rcsb pdb:3DY7|rcsb pdb:3E8N|rcsb pdb:3EQB|rcsb pdb:3EQC|rcsb pdb:3EQD|rcsb pdb:3EQF|rcsb pdb:3EQG|rcsb pdb:3EQH|rcsb pdb:3EQI|rcsb pdb:3MBL|rcsb pdb:3ORN|rcsb pdb:3OS3|rcsb pdb:3PP1|rcsb pdb:3SLS|rcsb pdb:3V01|rcsb pdb:3V04|rcsb pdb:3VVH|rcsb pdb:3W8Q|rcsb pdb:3WIG|rcsb pdb:3ZLS|rcsb pdb:3ZLW|rcsb pdb:3ZLX|rcsb pdb:3ZLY|rcsb pdb:3ZM4|rcsb pdb:4AN2|rcsb pdb:4AN3|rcsb pdb:4AN9|rcsb pdb:4ANB|rcsb pdb:4ARK|rcsb pdb:4LMN|rcsb pdb:4MNE|rcsb pdb:4U7Z|rcsb pdb:4U80|rcsb pdb:4U81|rcsb pdb:5EYM|rcsb pdb:5HZE|rcsb pdb:5YT3|rcsb pdb:6NYB|rcsb pdb:6PP9|rcsb pdb:6Q0J|rcsb pdb:6Q0T|rcsb pdb:6U2G|rcsb pdb:6V2W|rcsb pdb:6X2P|rcsb pdb:6X2S|rcsb pdb:6X2X|rcsb pdb:7B3M|rcsb pdb:7B7R|rcsb pdb:7B94|rcsb pdb:7B9L|rcsb pdb:7M0T|rcsb pdb:7M0U|rcsb pdb:7M0V|rcsb pdb:7M0W|rcsb pdb:7M0X|rcsb pdb:7M0Y|rcsb pdb:7M0Z|reactome:R-HSA-110056|reactome:R-HSA-170968|reactome:R-HSA-445144|reactome:R-HSA-5210891|reactome:R-HSA-5673000|reactome:R-HSA-5674135|reactome:R-HSA-5674499|reactome:R-HSA-5684264|reactome:R-HSA-6802946|reactome:R-HSA-6802948|reactome:R-HSA-6802952|reactome:R-HSA-6802955|reactome:R-HSA-9649948|reactome:R-HSA-9652169|reactome:R-HSA-9656223|rcsb pdb:5BX0	-	-	-	figure legend:6D|agonist:The MEK inhibitor  PD0325901|antagonist:Trametinib - a newer MEK inhibitor|comment:weak interaction|variable_condition:0 hours|variable_condition_2:PD0325901|dataset:Cancer - Interactions investigated in the context of cancer|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:9606(human-a_549)|taxid:9606(Homo sapiens Caucasian lung carcinoma cell line)	-	2014/06/12	2015/05/22	rogid:sfzILpNpX8UFB/vgH9LOKLpl/+g9606	rogid:ieBLcDINn9hZFZZEIQ3Ktdf2NF89606	rigid:Xwk0BUGvJhVn2aM8KIPYAmQJ6uU	false	-	-	-	-	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)
uniprotkb:Q02750	uniprotkb:P04049	intact:EBI-492564|ensembl:ENSP00000302486	intact:EBI-365996|uniprotkb:B0LPH8|uniprotkb:Q15278|uniprotkb:Q9UC20|ensembl:ENSP00000251849|uniprotkb:B2R5N3	psi-mi:mp2k1_human(display_long)|uniprotkb:ERK activator kinase 1(gene name synonym)|uniprotkb:MAPK/ERK kinase 1(gene name synonym)|uniprotkb:MAP2K1(gene name)|psi-mi:MAP2K1(display_short)|uniprotkb:MEK1(gene name synonym)|uniprotkb:PRKMK1(gene name synonym)	psi-mi:raf1_human(display_long)|uniprotkb:RAF1(gene name)|psi-mi:RAF1(display_short)|uniprotkb:RAF(gene name synonym)|uniprotkb:Proto-oncogene c-RAF(gene name synonym)|uniprotkb:Raf-1(gene name synonym)	psi-mi:"MI:0006"(anti bait coimmunoprecipitation)	Lito et al. (2014)	pubmed:24746704|imex:IM-22983	taxid:9606(human)|taxid:9606(Homo sapiens)	taxid:9606(human)|taxid:9606(Homo sapiens)	psi-mi:"MI:0915"(physical association)	psi-mi:"MI:0469"(IntAct)	intact:EBI-10823688|imex:IM-22983-15	-	-	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0499"(unspecified role)	psi-mi:"MI:0496"(bait)	psi-mi:"MI:0498"(prey)	psi-mi:"MI:0326"(protein)	psi-mi:"MI:0326"(protein)	refseq:NP_002746.1|refseq:XP_016877900.1|dip:DIP-201N|ensembl:ENSG00000169032(gene)|ensembl:ENST00000307102(transcript)|go:"GO:0000187"(obsolete activation of MAPK activity)|go:"GO:0004672"(protein kinase activity)|go:"GO:0004674"(protein serine/threonine kinase activity)|go:"GO:0004708"(MAP kinase kinase activity)|go:"GO:0004712"(protein serine/threonine/tyrosine kinase activity)|go:"GO:0004714"(transmembrane receptor protein tyrosine kinase activity)|go:"GO:0005078"(MAP-kinase scaffold activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005634"(nucleus)|go:"GO:0005739"(mitochondrion)|go:"GO:0005769"(early endosome)|go:"GO:0005770"(late endosome)|go:"GO:0005783"(endoplasmic reticulum)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005815"(microtubule organizing center)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0005925"(focal adhesion)|go:"GO:0006468"(protein phosphorylation)|go:"GO:0006935"(chemotaxis)|go:"GO:0007165"(signal transduction)|go:"GO:0007507"(heart development)|go:"GO:0008022"(protein C-terminus binding)|go:"GO:0008285"(negative regulation of cell population proliferation)|go:"GO:0010628"(positive regulation of gene expression)|go:"GO:0018107"(peptidyl-threonine phosphorylation)|go:"GO:0021697"(cerebellar cortex formation)|go:"GO:0030182"(neuron differentiation)|go:"GO:0030216"(keratinocyte differentiation)|go:"GO:0030878"(thyroid gland development)|go:"GO:0032872"(regulation of stress-activated MAPK cascade)|go:"GO:0043539"(protein serine/threonine kinase activator activity)|go:"GO:0045893"(positive regulation of transcription, DNA-templated)|go:"GO:0047485"(protein N-terminus binding)|go:"GO:0048538"(thymus development)|go:"GO:0048679"(regulation of axon regeneration)|go:"GO:0048870"(cell motility)|go:"GO:0050772"(positive regulation of axonogenesis)|go:"GO:0051726"(regulation of cell cycle)|go:"GO:0060020"(Bergmann glial cell differentiation)|go:"GO:0060324"(face development)|go:"GO:0060440"(trachea formation)|go:"GO:0060502"(epithelial cell proliferation involved in lung morphogenesis)|go:"GO:0060674"(placenta blood vessel development)|go:"GO:0060711"(labyrinthine layer development)|go:"GO:0070371"(ERK1 and ERK2 cascade)|go:"GO:0070374"(positive regulation of ERK1 and ERK2 cascade)|go:"GO:0071902"(positive regulation of protein serine/threonine kinase activity)|go:"GO:0090170"(regulation of Golgi inheritance)|go:"GO:0090398"(cellular senescence)|go:"GO:0097110"(scaffold protein binding)|go:"GO:0106310"(protein serine kinase activity)|go:"GO:0106311"|go:"GO:1903800"(positive regulation of production of miRNAs involved in gene silencing by miRNA)|go:"GO:2000641"(regulation of early endosome to late endosome transport)|interpro:IPR000719(Protein kinase, core)|interpro:IPR008271(Serine/threonine protein kinase, active site)|interpro:IPR011009(Protein kinase-like)|interpro:IPR017441(Protein kinase ATP binding, conserved site)|mint:Q02750|rcsb pdb:1S9J|rcsb pdb:2P55|rcsb pdb:3DV3|rcsb pdb:3DY7|rcsb pdb:3E8N|rcsb pdb:3EQB|rcsb pdb:3EQC|rcsb pdb:3EQD|rcsb pdb:3EQF|rcsb pdb:3EQG|rcsb pdb:3EQH|rcsb pdb:3EQI|rcsb pdb:3MBL|rcsb pdb:3ORN|rcsb pdb:3OS3|rcsb pdb:3PP1|rcsb pdb:3SLS|rcsb pdb:3V01|rcsb pdb:3V04|rcsb pdb:3VVH|rcsb pdb:3W8Q|rcsb pdb:3WIG|rcsb pdb:3ZLS|rcsb pdb:3ZLW|rcsb pdb:3ZLX|rcsb pdb:3ZLY|rcsb pdb:3ZM4|rcsb pdb:4AN2|rcsb pdb:4AN3|rcsb pdb:4AN9|rcsb pdb:4ANB|rcsb pdb:4ARK|rcsb pdb:4LMN|rcsb pdb:4MNE|rcsb pdb:4U7Z|rcsb pdb:4U80|rcsb pdb:4U81|rcsb pdb:5EYM|rcsb pdb:5HZE|rcsb pdb:5YT3|rcsb pdb:6NYB|rcsb pdb:6PP9|rcsb pdb:6Q0J|rcsb pdb:6Q0T|rcsb pdb:6U2G|rcsb pdb:6V2W|rcsb pdb:6X2P|rcsb pdb:6X2S|rcsb pdb:6X2X|rcsb pdb:7B3M|rcsb pdb:7B7R|rcsb pdb:7B94|rcsb pdb:7B9L|rcsb pdb:7M0T|rcsb pdb:7M0U|rcsb pdb:7M0V|rcsb pdb:7M0W|rcsb pdb:7M0X|rcsb pdb:7M0Y|rcsb pdb:7M0Z|reactome:R-HSA-110056|reactome:R-HSA-170968|reactome:R-HSA-445144|reactome:R-HSA-5210891|reactome:R-HSA-5673000|reactome:R-HSA-5674135|reactome:R-HSA-5674499|reactome:R-HSA-5684264|reactome:R-HSA-6802946|reactome:R-HSA-6802948|reactome:R-HSA-6802952|reactome:R-HSA-6802955|reactome:R-HSA-9649948|reactome:R-HSA-9652169|reactome:R-HSA-9656223|rcsb pdb:5BX0	rcsb pdb:3IQV|rcsb pdb:3KUC|rcsb pdb:3KUD|rcsb pdb:3NKX|rcsb pdb:3O8I|rcsb pdb:3OMV|rcsb pdb:4FJ3|rcsb pdb:4G0N|rcsb pdb:4G3X|rcsb pdb:4IEA|rcsb pdb:4IHL|rcsb pdb:6NTC|rcsb pdb:6NTD|rcsb pdb:6PTS|rcsb pdb:6PTW|rcsb pdb:6VJJ|rcsb pdb:6XGU|rcsb pdb:6XGV|rcsb pdb:6XHA|rcsb pdb:6XHB|rcsb pdb:6XI7|ensembl:ENSG00000132155(gene)|ensembl:ENST00000251849(transcript)|go:"GO:0000186"(obsolete activation of MAPKK activity)|go:"GO:0001666"(response to hypoxia)|go:"GO:0004672"(protein kinase activity)|go:"GO:0004674"(protein serine/threonine kinase activity)|go:"GO:0004709"(MAP kinase kinase kinase activity)|go:"GO:0005524"(ATP binding)|go:"GO:0005737"(cytoplasm)|go:"GO:0005739"(mitochondrion)|go:"GO:0005741"(mitochondrial outer membrane)|go:"GO:0005794"(Golgi apparatus)|go:"GO:0005829"(cytosol)|go:"GO:0005886"(plasma membrane)|go:"GO:0006468"(protein phosphorylation)|go:"GO:0006915"(apoptotic process)|go:"GO:0007165"(signal transduction)|go:"GO:0007190"(activation of adenylate cyclase activity)|go:"GO:0007507"(heart development)|go:"GO:0008179"(adenylate cyclase binding)|go:"GO:0008285"(negative regulation of cell population proliferation)|go:"GO:0010856"(adenylate cyclase activator activity)|go:"GO:0016607"(nuclear speck)|go:"GO:0019899"(enzyme binding)|go:"GO:0030154"(cell differentiation)|go:"GO:0030878"(thyroid gland development)|go:"GO:0031143"(pseudopodium)|go:"GO:0031333"(negative regulation of protein-containing complex assembly)|go:"GO:0031434"(mitogen-activated protein kinase kinase binding)|go:"GO:0033138"(positive regulation of peptidyl-serine phosphorylation)|go:"GO:0035019"(somatic stem cell population maintenance)|go:"GO:0035023"(regulation of Rho protein signal transduction)|go:"GO:0035773"(insulin secretion involved in cellular response to glucose stimulus)|go:"GO:0035994"(response to muscle stretch)|go:"GO:0042060"(wound healing)|go:"GO:0042802"(identical protein binding)|go:"GO:0042981"(regulation of apoptotic process)|go:"GO:0043066"(negative regulation of apoptotic process)|go:"GO:0043154"(negative regulation of cysteine-type endopeptidase activity involved in apoptotic process)|go:"GO:0044877"(protein-containing complex binding)|go:"GO:0045104"(intermediate filament cytoskeleton organization)|go:"GO:0045595"(regulation of cell differentiation)|go:"GO:0045944"(positive regulation of transcription by RNA polymerase II)|go:"GO:0046872"(metal ion binding)|go:"GO:0048011"(neurotrophin TRK receptor signaling pathway)|go:"GO:0048538"(thymus development)|go:"GO:0060324"(face development)|go:"GO:0071550"(death-inducing signaling complex assembly)|go:"GO:0106310"(protein serine kinase activity)|go:"GO:0106311"|go:"GO:1902042"(negative regulation of extrinsic apoptotic signaling pathway via death domain receptors)|go:"GO:2000145"(regulation of cell motility)|interpro:IPR000719(Protein kinase, core)|interpro:IPR001245(Tyrosine protein kinase)|interpro:IPR002219(Protein kinase C, phorbol ester/diacylglycerol binding)|interpro:IPR003116(Raf-like Ras-binding)|interpro:IPR008271(Serine/threonine protein kinase, active site)|interpro:IPR011009(Protein kinase-like)|interpro:IPR017441(Protein kinase ATP binding, conserved site)|interpro:IPR020454|interpro:IPR029071|mint:P04049|rcsb pdb:1C1Y|rcsb pdb:1FAQ|rcsb pdb:1FAR|rcsb pdb:1GUA|rcsb pdb:1RFA|rcsb pdb:3CU8|rcsb pdb:3IQJ|rcsb pdb:3IQU|rcsb pdb:7JHP|reactome:R-HSA-2672351|reactome:R-HSA-392517|reactome:R-HSA-430116|reactome:R-HSA-5621575|reactome:R-HSA-5673000|reactome:R-HSA-5674135|reactome:R-HSA-5674499|reactome:R-HSA-5675221|reactome:R-HSA-6802946|reactome:R-HSA-6802948|reactome:R-HSA-6802952|reactome:R-HSA-6802955|reactome:R-HSA-9649948|reactome:R-HSA-9656223|reactome:R-HSA-9660537|refseq:NP_002871.1|refseq:XP_011532276.1|refseq:XP_005265412.1|dip:DIP-1048N	-	-	-	figure legend:6D|agonist:The MEK inhibitor  PD0325901|antagonist:Trametinib - a newer MEK inhibitor|comment:weak interaction|variable_condition:48 hours|variable_condition_2:Trametinib|dataset:Cancer - Interactions investigated in the context of cancer|full coverage:Only protein-protein interactions|curation depth:imex curation	taxid:9606(human-a_549)|taxid:9606(Homo sapiens Caucasian lung carcinoma cell line)	-	2014/06/12	2015/05/26	rogid:ieBLcDINn9hZFZZEIQ3Ktdf2NF89606	rogid:1WOAPuzcZdMoq9hyFD/qr4Tz7049606	rigid:PBGsilY2R+EvyAAA1Mf4R6U2sdY	false	-	-	-	-	psi-mi:"MI:0113"(western blot)	psi-mi:"MI:0113"(western blot)